Rw3G024640

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0012763
Physical Location & Seq
Reverse (-)
31837379 .. 31839241
1863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G024640.1

Sequence Viewer

Length: 1416 bp
ATGAAGAAGCTTTCCAAATTGTCACTAATGTGCGTTTTATTCTTTGGCTTCTTTCTTCTGTCAACCCAAGCCAGATGGCATAACCATACCAAACACAGCAAGCACAACCACGCTCACAAGATTTCTTACATTTCACAGCCTCCTTCCACTTCACCTGGACCTACAGCTAGCTATAGTCCTCCTCCCGAAGAGCCTAAGAATCCATACAACTCTACACCTGGTGTTTTTGATGTTCGATCATATGGGGCTGTTGGGGATGGCCAAACAGATGACACACAGGCATTTAAGATGGCTTGGGACACAGCCTGCCAAAGTGAATCACCAGCAAAGATACTTGTTCCGAACCATTTCACATTCATGATTCAGTCCACAATATTTACCGGTCCCTGCCTTTCAGGCCTGGTGTTACAGGTTGATGGGACTATAGTACCACCTGATGGACCAGAGTCCTGGCCAAAAAACAACAGCAGGCGCCAATGGCTCGTCTTTTATCGAATCAACGAGATGTCGCTGCAAGGGGGTGGTGTTATAGATGGGAGAGGAGACAAATGGTGGCATCTTCCCTGCAAACCCCACATGGGGATACATGGAACCACCTTGCCCGGACCATGTGATAGCCCAGTTGCCATTAGGTTTTTCATGAGCTCTAACTTGACAGTACAAGGTCTTACGATTAAGAACAGCCCCCAATTCCACTTCAGATTTGATGGTTGCAGGAATGTGCATGTAGAGTCCATTTCTATATCAGCTCCTGCTCGAAGTCCAAATACAGATGGGATTCACATAGAGAATACTAATGATGTCAAAATTTATAATTCAGTCATTTCTAATGGTGATGATTGTGTATCAATTGGATCAGGCTGTTATGATGTAGACATAAGAAACATCACTTGTGGTCCTGGTCATGGAATCAGCATTGGGAGTCTAGGAAACCACAACTCGCGAGCATGTGTGAAAAATATTACAGTTCGAGACTCAATAATTAGAGTTTCGGATAATGGAGTTCGGATCAAGACATGGCAGGGTGGATCCGGAGCAGTATCTGGAGTAACATTCAGTAACATTCACATGGACAATGTTAGGAACCCGATAATGATTGATCAATTTTACTGCCTTACTAAGAAGTGCATCAATCAAACTGCAGCGGTATATGTATCGGACATCCTATACTCGGGCATAAAGGGAACCTATGATGTCCGGAATGCTCCAATGCATTTTGCCTGCAGTGATTCTCTGCCATGCACTAACTTAACACTCTCAGATGTTGAGCTTCTTCCTGCTGTAGGAGATATGATGTCAGACCCTTATTGTTGGAATGCTTATGGACAACTGCAAACGCTTACTATTCCGCCGGTCTCTTGCTTGTTGGAGGGTACTCCTAGATCTTTACTGAGCGATGAAATAGACCATTGCTGA

Protein Analysis

471

Amino Acids

51.6

Weight (kDa)

6.79

Isoelectric Point (pI)

52.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 103 - 425 3.9e-92 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012763)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02460
fragaria_vesca FvH4_1g26650
malus_domestica MD09G1287300.v1.1 MD17G1279500.v1.1
prunus_persica Prupe.3G005900_v2.0.a1 Prupe.6G307200_v2.0.a1
pyrus_communis pycom09g19460 pycom17g27760
rosa_chinensis RchiOBHm_Chr3g0486841
rosa_laevigata RLG00000023055
rosa_multiflora Rmu_sc0005709.1_g000005
rosa_roxburghii Rroxscaffold_6G00395520
rosa_rugosa Rorug03G0227400.1
rosa_samantha Rh3AG277500 Rh3BG312600 Rh3CG311500 Rh3DG308100
rosa_wichuraiana Rw3G024640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 813
AccB1I GGYRCC 1 cut(s) 471
AccB7I CCANNNNNTGG 1 cut(s) 437
AccI GTMKAC 1 cut(s) 873
AccII CGCG 1 cut(s) 943
AccIII TCCGGA 2 cut(s) 1031, 1197
AciI CCGC 2 cut(s) 1145, 1349
AclWI GGATC 4 cut(s) 862, 1016, 1023, 1036
AcoI YGGCCR 2 cut(s) 259, 452
AcsI RAATTY 1 cut(s) 807
AcuI CTGAAG 1 cut(s) 682
AcyI GRCGYC 1 cut(s) 472
AdeI CACNNNGTG 1 cut(s) 221
AfaI GTAC 3 cut(s) 429, 660, 1375
AfiI CCNNNNNNNGG 6 cut(s) 437, 578, 579, 905, 1171, 1283
AgeI ACCGGT 1 cut(s) 380
AjnI CCWGG 5 cut(s) 154, 217, 399, 449, 898
AleI CACNNNNGTG 1 cut(s) 28
AluBI AGCT 6 cut(s) 10, 167, 171, 645, 749, 1270
AluI AGCT 6 cut(s) 10, 167, 171, 645, 749, 1270
Alw21I GWGCWC 1 cut(s) 647
Alw26I GTCTC 3 cut(s) 537, 966, 1360
AlwI GGATC 4 cut(s) 862, 1016, 1023, 1036
AlwNI CAGNNNCTG 2 cut(s) 752, 1043
Ama87I CYCGRG 1 cut(s) 1171
Aor13HI TCCGGA 2 cut(s) 1031, 1197
AoxI GGCC 3 cut(s) 259, 397, 452
ApeKI GCWGC 2 cut(s) 511, 1142
ApoI RAATTY 1 cut(s) 807
AsiGI ACCGGT 1 cut(s) 380
Asp700I GAANNNNTTC 1 cut(s) 347
AspLEI GCGC 1 cut(s) 474
AspS9I GGNCC 5 cut(s) 158, 383, 440, 605, 896
AsuC2I CCSGG 1 cut(s) 603
AsuHPI GGTGA 3 cut(s) 144, 312, 845
AsuNHI GCTAGC 1 cut(s) 167
AvaI CYCGRG 1 cut(s) 1171
AvaII GGWCC 5 cut(s) 158, 383, 440, 605, 896
BaeI ACNNNNGTAYC 2 cut(s) 411, 444
BalI TGGCCA 2 cut(s) 261, 454
BamHI GGATCC 1 cut(s) 1028
BanI GGYRCC 1 cut(s) 471
BanII GRGCYC 1 cut(s) 647
BarI GAAGNNNNNNTAC 2 cut(s) 751, 783
Bbv12I GWGCWC 1 cut(s) 647
BbvI GCAGC 2 cut(s) 498, 1154
BccI CCATC 8 cut(s) 69, 251, 283, 410, 431, 527, 701, 767
BciT130I CCWGG 5 cut(s) 156, 219, 401, 451, 900
BciVI GTATCC 1 cut(s) 576
BclI TGATCA 1 cut(s) 1099
BcnI CCSGG 1 cut(s) 603
BcoDI GTCTC 3 cut(s) 537, 966, 1360
BfaI CTAG 3 cut(s) 168, 926, 1380
BfmI CTRYAG 6 cut(s) 162, 172, 423, 1140, 1222, 1281
BfoI RGCGCY 1 cut(s) 475
BfuI GTATCC 1 cut(s) 576
BglI GCCNNNNNGGC 1 cut(s) 396
BglII AGATCT 1 cut(s) 1382
BisI GCNGC 2 cut(s) 512, 1143
BlsI GCNGC 2 cut(s) 513, 1144
Bme1390I CCNGG 6 cut(s) 156, 219, 401, 451, 603, 900
Bme18I GGWCC 5 cut(s) 158, 383, 440, 605, 896
BmeT110I CYCGRG 1 cut(s) 1171
BmgT120I GGNCC 5 cut(s) 158, 383, 440, 605, 896
BmiI GGNNCC 6 cut(s) 385, 473, 592, 1030, 1085, 1186
BmrFI CCNGG 6 cut(s) 156, 219, 401, 451, 603, 900
BmrI ACTGGG 1 cut(s) 614
BmsI GCATC 2 cut(s) 565, 1137
BmtI GCTAGC 1 cut(s) 171
BmuI ACTGGG 1 cut(s) 614
BoxI GACNNNNGTC 1 cut(s) 445
BpmI CTGGAG 1 cut(s) 1065
BpuMI CCSGG 1 cut(s) 603
BsaHI GRCGYC 1 cut(s) 472
BsaI GGTCTC 1 cut(s) 1360
BsaWI WCCGGW 3 cut(s) 380, 1031, 1197
Bsc4I CCNNNNNNNGG 6 cut(s) 437, 578, 579, 905, 1171, 1283
Bse118I RCCGGY 2 cut(s) 380, 1351
Bse1I ACTGG 1 cut(s) 620
Bse3DI GCAATG 1 cut(s) 1408
BseAI TCCGGA 2 cut(s) 1031, 1197
BseBI CCWGG 5 cut(s) 156, 219, 401, 451, 900
BseGI GGATG 2 cut(s) 262, 1161
BseLI CCNNNNNNNGG 6 cut(s) 437, 578, 579, 905, 1171, 1283
BseMI GCAATG 1 cut(s) 1408
BseMII CTCAG 2 cut(s) 1272, 1382
BseNI ACTGG 1 cut(s) 620
BseRI GAGGAG 2 cut(s) 171, 555
BseXI GCAGC 2 cut(s) 498, 1154
Bsh1236I CGCG 1 cut(s) 943
BshFI GGCC 3 cut(s) 261, 399, 454
BshNI GGYRCC 1 cut(s) 471
BshTI ACCGGT 1 cut(s) 380
BsiHKAI GWGCWC 1 cut(s) 647
BsiHKCI CYCGRG 1 cut(s) 1171
BsiSI CCGG 5 cut(s) 381, 603, 1032, 1198, 1352
BslFI GGGAC 3 cut(s) 311, 369, 433
BslI CCNNNNNNNGG 6 cut(s) 437, 578, 579, 905, 1171, 1283
BsmAI GTCTC 3 cut(s) 537, 966, 1360
BsmFI GGGAC 3 cut(s) 311, 369, 433
BsmI GAATGC 2 cut(s) 1207, 1321
BsnI GGCC 3 cut(s) 261, 399, 454
Bso31I GGTCTC 1 cut(s) 1360
BsoBI CYCGRG 1 cut(s) 1171
Bsp1286I GDGCHC 1 cut(s) 647
Bsp13I TCCGGA 2 cut(s) 1031, 1197
Bsp143I GATC 6 cut(s) 236, 854, 1008, 1028, 1099, 1382
Bsp68I TCGCGA 1 cut(s) 943
BspACI CCGC 2 cut(s) 1145, 1349
BspANI GGCC 3 cut(s) 261, 399, 454
BspCNI CTCAG 2 cut(s) 1271, 1383
BspEI TCCGGA 2 cut(s) 1031, 1197
BspFNI CGCG 1 cut(s) 943
BspHI TCATGA 2 cut(s) 357, 639
BspLI GGNNCC 6 cut(s) 385, 473, 592, 1030, 1085, 1186
BspMAI CTGCAG 2 cut(s) 1144, 1226
BspOI GCTAGC 1 cut(s) 171
BspPI GGATC 4 cut(s) 862, 1016, 1023, 1036
BspQI GCTCTTC 1 cut(s) 183
BspT107I GGYRCC 1 cut(s) 471
BspTNI GGTCTC 1 cut(s) 1360
BsrDI GCAATG 1 cut(s) 1408
BsrFI RCCGGY 2 cut(s) 380, 1351
BsrI ACTGG 1 cut(s) 620
BssAI RCCGGY 2 cut(s) 380, 1351
BssMI GATC 6 cut(s) 236, 854, 1008, 1028, 1099, 1382
BssNI GRCGYC 1 cut(s) 472
Bst2UI CCWGG 5 cut(s) 156, 219, 401, 451, 900
Bst4CI ACNGT 2 cut(s) 658, 967
Bst6I CTCTTC 1 cut(s) 183
BstACI GRCGYC 1 cut(s) 472
BstC8I GCNNGC 6 cut(s) 101, 169, 307, 470, 945, 1222
BstDEI CTNAG 4 cut(s) 195, 1119, 1258, 1391
BstENI CCTNNNNNAGG 1 cut(s) 1281
BstF5I GGATG 2 cut(s) 262, 1161
BstFNI CGCG 1 cut(s) 943
BstH2I RGCGCY 1 cut(s) 475
BstHHI GCGC 1 cut(s) 474
BstKTI GATC 6 cut(s) 239, 857, 1011, 1031, 1102, 1385
BstMAI GTCTC 3 cut(s) 537, 966, 1360
BstMBI GATC 6 cut(s) 236, 854, 1008, 1028, 1099, 1382
BstMWI GCNNNNNNNGC 2 cut(s) 396, 478
BstNI CCWGG 5 cut(s) 156, 219, 401, 451, 900
BstNSI RCATGY 2 cut(s) 728, 951
BstPAI GACNNNNGTC 1 cut(s) 445
BstSCI CCNGG 6 cut(s) 154, 217, 399, 449, 601, 898
BstSFI CTRYAG 6 cut(s) 162, 172, 423, 1140, 1222, 1281
BstUI CGCG 1 cut(s) 943
BstV1I GCAGC 2 cut(s) 498, 1154
BstX2I RGATCY 2 cut(s) 1028, 1382
BstXI CCANNNNNNTGG 1 cut(s) 450
BstYI RGATCY 2 cut(s) 1028, 1382
BsuI GTATCC 1 cut(s) 576
BsuRI GGCC 3 cut(s) 261, 399, 454
BtgZI GCGATG 1 cut(s) 1410
BtsCI GGATG 2 cut(s) 262, 1161
BtsI GCAGTG 1 cut(s) 1231
BtsIMutI CAGTG 1 cut(s) 1231
BtuMI TCGCGA 1 cut(s) 943
Cac8I GCNNGC 6 cut(s) 101, 169, 307, 470, 945, 1222
CaiI CAGNNNCTG 2 cut(s) 752, 1043
CciI TCATGA 2 cut(s) 357, 639
CfoI GCGC 1 cut(s) 474
Cfr10I RCCGGY 2 cut(s) 380, 1351
Cfr13I GGNCC 5 cut(s) 158, 383, 440, 605, 896
CsiI ACCWGGT 1 cut(s) 217
Csp6I GTAC 3 cut(s) 428, 659, 1374
CspAI ACCGGT 1 cut(s) 380
CviQI GTAC 3 cut(s) 428, 659, 1374
DdeI CTNAG 4 cut(s) 195, 1119, 1258, 1391
DinI GGCGCC 1 cut(s) 473
DpnI GATC 6 cut(s) 238, 856, 1010, 1030, 1101, 1384
DpnII GATC 6 cut(s) 236, 854, 1008, 1028, 1099, 1382
DraIII CACNNNGTG 1 cut(s) 221
EaeI YGGCCR 2 cut(s) 259, 452
Eam1104I CTCTTC 1 cut(s) 183
EarI CTCTTC 1 cut(s) 183
EciI GGCGGA 1 cut(s) 1338
Ecl136II GAGCTC 1 cut(s) 645
Eco147I AGGCCT 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 647
Eco31I GGTCTC 1 cut(s) 1360
Eco47I GGWCC 5 cut(s) 158, 383, 440, 605, 896
Eco53kI GAGCTC 1 cut(s) 645
Eco57I CTGAAG 1 cut(s) 682
Eco88I CYCGRG 1 cut(s) 1171
EcoICRI GAGCTC 1 cut(s) 645
EcoNI CCTNNNNNAGG 1 cut(s) 1281
EcoRII CCWGG 5 cut(s) 154, 217, 399, 449, 898
EcoT22I ATGCAT 1 cut(s) 1215
EcoT38I GRGCYC 1 cut(s) 647
EgeI GGCGCC 1 cut(s) 473
EheI GGCGCC 1 cut(s) 473
FaqI GGGAC 3 cut(s) 311, 369, 433
FauNDI CATATG 1 cut(s) 241
FbaI TGATCA 1 cut(s) 1099
FblI GTMKAC 1 cut(s) 873
Fnu4HI GCNGC 2 cut(s) 512, 1143
FokI GGATG 2 cut(s) 269, 1148
FriOI GRGCYC 1 cut(s) 647
Fsp4HI GCNGC 2 cut(s) 512, 1143
FspBI CTAG 3 cut(s) 168, 926, 1380
GlaI GCGC 1 cut(s) 473
GluI GCNGC 2 cut(s) 512, 1143
GsuI CTGGAG 1 cut(s) 1065
HaeII RGCGCY 1 cut(s) 475
HaeIII GGCC 3 cut(s) 261, 399, 454
HapII CCGG 5 cut(s) 381, 603, 1032, 1198, 1352
HhaI GCGC 1 cut(s) 474
Hin1I GRCGYC 1 cut(s) 472
Hin6I GCGC 1 cut(s) 472
HinP1I GCGC 1 cut(s) 472
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HindIII AAGCTT 1 cut(s) 8
HpaII CCGG 5 cut(s) 381, 603, 1032, 1198, 1352
HphI GGTGA 3 cut(s) 144, 312, 845
Hpy166II GTNNAC 3 cut(s) 63, 369, 874
Hpy188I TCNGA 7 cut(s) 342, 701, 994, 1008, 1159, 1261, 1300
Hpy188III TCNNGA 9 cut(s) 185, 358, 640, 942, 971, 1012, 1032, 1044, 1198
Hpy8I GTNNAC 3 cut(s) 63, 369, 874
HpyAV CCTTC 1 cut(s) 153
HpyCH4III ACNGT 2 cut(s) 658, 967
HpyF10VI GCNNNNNNNGC 2 cut(s) 396, 478
HpyF3I CTNAG 4 cut(s) 195, 1119, 1258, 1391
Hsp92I GRCGYC 1 cut(s) 472
HspAI GCGC 1 cut(s) 472
KasI GGCGCC 1 cut(s) 471
Kpn2I TCCGGA 2 cut(s) 1031, 1197
Ksp22I TGATCA 1 cut(s) 1099
Kzo9I GATC 6 cut(s) 236, 854, 1008, 1028, 1099, 1382
LguI GCTCTTC 1 cut(s) 183
LmnI GCTCC 3 cut(s) 754, 1034, 1210
Lsp1109I GCAGC 2 cut(s) 498, 1154
LweI GCATC 2 cut(s) 565, 1137
MabI ACCWGGT 1 cut(s) 217
MaeI CTAG 3 cut(s) 168, 926, 1380
MaeIII GTNAC 4 cut(s) 21, 405, 1048, 1058
MalI GATC 6 cut(s) 238, 856, 1010, 1030, 1101, 1384
MboI GATC 6 cut(s) 236, 854, 1008, 1028, 1099, 1382
MboII GAAGA 5 cut(s) 16, 47, 200, 551, 1265
MfeI CAATTG 1 cut(s) 849
MflI RGATCY 2 cut(s) 1028, 1382
MhlI GDGCHC 1 cut(s) 647
MlsI TGGCCA 2 cut(s) 261, 454
MluCI AATT 7 cut(s) 17, 689, 807, 814, 849, 981, 1103
MluNI TGGCCA 2 cut(s) 261, 454
Mly113I GGCGCC 1 cut(s) 472
MlyI GAGTC 4 cut(s) 455, 740, 931, 968
MmeI TCCRAC 2 cut(s) 1292, 1347
MnlI CCTC 5 cut(s) 150, 189, 192, 533, 1363
Mox20I TGGCCA 2 cut(s) 261, 454
Mph1103I ATGCAT 1 cut(s) 1215
MroI TCCGGA 2 cut(s) 1031, 1197
MroXI GAANNNNTTC 1 cut(s) 347
MscI TGGCCA 2 cut(s) 261, 454
MseI TTAA 3 cut(s) 285, 675, 1250
MslI CAYNNNNRTG 3 cut(s) 28, 356, 1067
Msp20I TGGCCA 2 cut(s) 261, 454
MspA1I CMGCKG 1 cut(s) 1145
MspI CCGG 5 cut(s) 381, 603, 1032, 1198, 1352
MspR9I CCNGG 6 cut(s) 156, 219, 401, 451, 603, 900
MunI CAATTG 1 cut(s) 849
Mva1269I GAATGC 2 cut(s) 1207, 1321
MvaI CCWGG 5 cut(s) 156, 219, 401, 451, 900
MvnI CGCG 1 cut(s) 943
MwoI GCNNNNNNNGC 2 cut(s) 396, 478
NarI GGCGCC 1 cut(s) 472
NciI CCSGG 1 cut(s) 603
NdeI CATATG 1 cut(s) 241
NdeII GATC 6 cut(s) 236, 854, 1008, 1028, 1099, 1382
NheI GCTAGC 1 cut(s) 167
NlaIV GGNNCC 6 cut(s) 385, 473, 592, 1030, 1085, 1186
NmuCI GTSAC 1 cut(s) 21
NruI TCGCGA 1 cut(s) 943
NsiI ATGCAT 1 cut(s) 1215
NspI RCATGY 2 cut(s) 728, 951
OliI CACNNNNGTG 1 cut(s) 28
PagI TCATGA 2 cut(s) 357, 639
PceI AGGCCT 1 cut(s) 399
PciSI GCTCTTC 1 cut(s) 183
PctI GAATGC 2 cut(s) 1207, 1321
PdmI GAANNNNTTC 1 cut(s) 347
PfeI GAWTC 7 cut(s) 199, 317, 361, 495, 778, 909, 1229
PflMI CCANNNNNTGG 1 cut(s) 437
PinAI ACCGGT 1 cut(s) 380
PkrI GCNGC 2 cut(s) 513, 1144
PleI GAGTC 4 cut(s) 454, 739, 930, 968
PluTI GGCGCC 1 cut(s) 475
PpsI GAGTC 4 cut(s) 454, 739, 930, 968
PshAI GACNNNNGTC 1 cut(s) 445
PsiI TTATAA 1 cut(s) 813
Psp124BI GAGCTC 1 cut(s) 647
Psp6I CCWGG 5 cut(s) 154, 217, 399, 449, 898
PspGI CCWGG 5 cut(s) 154, 217, 399, 449, 898
PspN4I GGNNCC 6 cut(s) 385, 473, 592, 1030, 1085, 1186
PspPI GGNCC 5 cut(s) 158, 383, 440, 605, 896
PstI CTGCAG 2 cut(s) 1144, 1226
PstNI CAGNNNCTG 2 cut(s) 752, 1043
PsuI RGATCY 2 cut(s) 1028, 1382
RruI TCGCGA 1 cut(s) 943
RsaI GTAC 3 cut(s) 429, 660, 1375
RsaNI GTAC 3 cut(s) 428, 659, 1374
RseI CAYNNNNRTG 3 cut(s) 28, 356, 1067
SacI GAGCTC 1 cut(s) 647
SapI GCTCTTC 1 cut(s) 183
SaqAI TTAA 3 cut(s) 285, 675, 1250
SatI GCNGC 2 cut(s) 512, 1143
Sau3AI GATC 6 cut(s) 236, 854, 1008, 1028, 1099, 1382
Sau96I GGNCC 5 cut(s) 158, 383, 440, 605, 896
SchI GAGTC 4 cut(s) 455, 740, 931, 968
ScrFI CCNGG 6 cut(s) 156, 219, 401, 451, 603, 900
SduI GDGCHC 1 cut(s) 647
SexAI ACCWGGT 1 cut(s) 217
SfaNI GCATC 2 cut(s) 565, 1137
SfcI CTRYAG 6 cut(s) 162, 172, 423, 1140, 1222, 1281
SfoI GGCGCC 1 cut(s) 473
SinI GGWCC 5 cut(s) 158, 383, 440, 605, 896
SmiMI CAYNNNNRTG 3 cut(s) 28, 356, 1067
Sse9I AATT 7 cut(s) 17, 689, 807, 814, 849, 981, 1103
SseBI AGGCCT 1 cut(s) 399
SsiI CCGC 2 cut(s) 1145, 1349
SspDI GGCGCC 1 cut(s) 471
SspI AATATT 2 cut(s) 375, 961
SspMI CTAG 3 cut(s) 168, 926, 1380
SstI GAGCTC 1 cut(s) 647
StuI AGGCCT 1 cut(s) 399
StyD4I CCNGG 6 cut(s) 154, 217, 399, 449, 601, 898
TaaI ACNGT 2 cut(s) 658, 967
TaqI TCGA 4 cut(s) 235, 493, 757, 970
TasI AATT 7 cut(s) 17, 689, 807, 814, 849, 981, 1103
TatI WGTACW 1 cut(s) 658
TfiI GAWTC 7 cut(s) 199, 317, 361, 495, 778, 909, 1229
Tru1I TTAA 3 cut(s) 285, 675, 1250
Tru9I TTAA 3 cut(s) 285, 675, 1250
TscAI CASTG 1 cut(s) 1231
TseFI GTSAC 1 cut(s) 21
TseI GCWGC 2 cut(s) 511, 1142
Tsp45I GTSAC 1 cut(s) 21
TspDTI ATGAA 4 cut(s) 17, 346, 628, 1413
TspRI CASTG 1 cut(s) 1231
Van91I CCANNNNNTGG 1 cut(s) 437
VpaK11BI GGWCC 5 cut(s) 158, 383, 440, 605, 896
XagI CCTNNNNNAGG 1 cut(s) 1281
XapI RAATTY 1 cut(s) 807
XceI RCATGY 2 cut(s) 728, 951
XmiI GTMKAC 1 cut(s) 873
XmnI GAANNNNTTC 1 cut(s) 347
XspI CTAG 3 cut(s) 168, 926, 1380
Zsp2I ATGCAT 1 cut(s) 1215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.