Prupe.3G005900_v2.0.a1

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0012763
Physical Location & Seq
Reverse (-)
354434 .. 356716
2283 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G005900.1

Sequence Viewer

Length: 1389 bp
ATGAAGAACTGCAAATTGTCACTAATGCAAGTGTTGTGCATTTCATTCTTTTGCTTCTTTCTGTCCACCCAAGCCAGACGGCATAACCATAAACACAAGCACACCCACCCTCACATTTCACAACCTCCTTCCCCTCCACCTGATTCTGCTAGCCCTCCTGAAGAGGCTACCAATCCTAATAATAGCTCCTCTGGTGTTTTCGATGTGCGGTCGTTTGGTGCTGTTGGAGATGGCCAAACAGATGACACTGAGGCATTTAAGATGGCTTGGGACACAGCTTGCCAAAGTGAATCTGCAGCAAGGATCCTTGTTCCCCGAGGTTTCTCATTCATGATTCAATCTACAATATTCACTGGTCCCTGCCTTGGGGGCCTGGTGTTACAGGTTGATGGAACTCTAGTGCCACCTGATGGACCAGAGTCTTGGCCAAAGAACGCCAGCAGGCATCAATGGCTTGTCTTTTACAGAATCAACGAGATGTCGTTGCAAGGGGGTGGTGTAATTGATGGGAGAGGAGAGAAATGGTGGAATCTTCCCTGCAAACCCCACAGGGGGATCAATGGAACCACATTGCCTGGACCATGTTACAGCCCAGTTGCCATTAAGTTCTTCATGAGCTCCAACTTGACAGTTCAAGGACTTAAAATCAAGAATAGCCCCCAATTCCACTTCAGATTTGATAACTGCAGGAATGTCCATATAGAATCGATTTCTATATCCGCCCCTGCTCGAAGTCCGAATACAGATGGGATTCACATAGAGAACACAAATGATGTCCAAATTTATAACTCAGTAATTTCAAATGGTGACGATTGTGTATCAATTGGGTCAGGCTGTTATGATGTAGACATACGAAACATCACTTGTGGACCTGGTCATGGAATCAGCATTGGGAGTCTAGGCAATCACAACTCGCGAGCATGCGTCTCTAACATTACAGTTCGCGACTCAATAATCAAAGAGACGGATAATGGAGTTAGGATCAAGACATGGCAGGGTGGATCCGGATCAGTTTCGGGAGTAACATTCAGTAACATTCACATGGATAATGTTAGGAACCCGATTATGATAAACCAATTCTACTGCCTTAACAAGGGGTGCACCAACCAAACTTCAGCAGTCTTTGTATCAGACATACTCTATTCAAACATAAGGGGAACCTACGATGTTCGGAGTTCACCGATGCATTTTGCTTGCAGTGATGCTCTCCCATGCACTAACTTGACACTCTCAGAAGTCGAGCTTCTTCCTGCTGTAGGAGACATAGTATTGGACCCGTATTGTTGGAATGCTTATGGAGAACTGCAGACACTAACAATTCCACCAGTCTCTTGCTTGTTGGAGGGTGTTCCTCGATCCCTACTGGACAATGACATTGAGCATTGCTGA

Protein Analysis

463

Amino Acids

50.48

Weight (kDa)

6.13

Isoelectric Point (pI)

57.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012763)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02460
fragaria_vesca FvH4_1g26650
malus_domestica MD09G1287300.v1.1 MD17G1279500.v1.1
prunus_persica Prupe.3G005900_v2.0.a1 Prupe.6G307200_v2.0.a1
pyrus_communis pycom09g19460 pycom17g27760
rosa_chinensis RchiOBHm_Chr3g0486841
rosa_laevigata RLG00000023055
rosa_multiflora Rmu_sc0005709.1_g000005
rosa_roxburghii Rroxscaffold_6G00395520
rosa_rugosa Rorug03G0227400.1
rosa_samantha Rh3AG277500 Rh3BG312600 Rh3CG311500 Rh3DG308100
rosa_wichuraiana Rw3G024640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 786
AccB7I CCANNNNNTGG 1 cut(s) 410
AccI GTMKAC 1 cut(s) 846
AccII CGCG 2 cut(s) 916, 945
AccIII TCCGGA 1 cut(s) 1004
AciI CCGC 2 cut(s) 208, 720
AclWI GGATC 8 cut(s) 298, 311, 563, 989, 996, 1009, 1015, 1350
AcoI YGGCCR 2 cut(s) 232, 425
AcsI RAATTY 1 cut(s) 780
AcuI CTGAAG 3 cut(s) 180, 655, 1098
AfiI CCNNNNNNNGG 8 cut(s) 365, 366, 410, 551, 552, 878, 1093, 1256
AgsI TTSAA 4 cut(s) 338, 635, 801, 1146
AjnI CCWGG 3 cut(s) 372, 574, 871
AluBI AGCT 4 cut(s) 186, 278, 618, 1243
AluI AGCT 4 cut(s) 186, 278, 618, 1243
Alw21I GWGCWC 2 cut(s) 620, 1103
Alw26I GTCTC 4 cut(s) 931, 956, 1254, 1333
Alw44I GTGCAC 1 cut(s) 1099
AlwI GGATC 8 cut(s) 298, 311, 563, 989, 996, 1009, 1015, 1350
Ama87I CYCGRG 1 cut(s) 315
Aor13HI TCCGGA 1 cut(s) 1004
AoxI GGCC 3 cut(s) 232, 370, 425
ApaLI GTGCAC 1 cut(s) 1099
ApeKI GCWGC 1 cut(s) 296
ApoI RAATTY 1 cut(s) 780
AspS9I GGNCC 6 cut(s) 356, 370, 413, 578, 869, 1273
AsuHPI GGTGA 2 cut(s) 818, 1170
AsuNHI GCTAGC 1 cut(s) 149
AvaI CYCGRG 1 cut(s) 315
AvaII GGWCC 5 cut(s) 356, 413, 578, 869, 1273
BaeGI GKGCMC 1 cut(s) 1103
BalI TGGCCA 2 cut(s) 234, 427
BamHI GGATCC 2 cut(s) 303, 1001
BanII GRGCYC 1 cut(s) 620
BarI GAAGNNNNNNTAC 2 cut(s) 724, 756
Bbv12I GWGCWC 2 cut(s) 620, 1103
BbvI GCAGC 1 cut(s) 308
BccI CCATC 6 cut(s) 224, 256, 383, 404, 500, 740
BceAI ACGGC 1 cut(s) 95
BciT130I CCWGG 3 cut(s) 374, 576, 873
BcoDI GTCTC 4 cut(s) 931, 956, 1254, 1333
BfaI CTAG 3 cut(s) 150, 398, 899
BfmI CTRYAG 4 cut(s) 294, 685, 1254, 1304
BglI GCCNNNNNGGC 1 cut(s) 369
BisI GCNGC 1 cut(s) 297
BlsI GCNGC 1 cut(s) 298
Bme1390I CCNGG 3 cut(s) 374, 576, 873
Bme18I GGWCC 5 cut(s) 356, 413, 578, 869, 1273
BmeT110I CYCGRG 1 cut(s) 315
BmgT120I GGNCC 6 cut(s) 356, 370, 413, 578, 869, 1273
BmiI GGNNCC 8 cut(s) 305, 358, 371, 565, 1003, 1058, 1159, 1275
BmrFI CCNGG 3 cut(s) 374, 576, 873
BmrI ACTGGG 1 cut(s) 587
BmsI GCATC 3 cut(s) 454, 1173, 1192
BmtI GCTAGC 1 cut(s) 153
BmuI ACTGGG 1 cut(s) 587
BoxI GACNNNNGTC 1 cut(s) 418
Bsa29I ATCGAT 1 cut(s) 707
BsaBI GATNNNNATC 1 cut(s) 1006
BsaJI CCNNGG 2 cut(s) 316, 364
BsaWI WCCGGW 1 cut(s) 1004
BsaXI ACNNNNNCTCC 2 cut(s) 1251, 1281
Bsc4I CCNNNNNNNGG 8 cut(s) 365, 366, 410, 551, 552, 878, 1093, 1256
Bse1I ACTGG 4 cut(s) 358, 593, 1325, 1368
Bse3DI GCAATG 2 cut(s) 569, 1381
Bse8I GATNNNNATC 1 cut(s) 1006
BseAI TCCGGA 1 cut(s) 1004
BseBI CCWGG 3 cut(s) 374, 576, 873
BseCI ATCGAT 1 cut(s) 707
BseDI CCNNGG 2 cut(s) 316, 364
BseJI GATNNNNATC 1 cut(s) 1006
BseLI CCNNNNNNNGG 8 cut(s) 365, 366, 410, 551, 552, 878, 1093, 1256
BseMI GCAATG 2 cut(s) 569, 1381
BseMII CTCAG 3 cut(s) 240, 804, 1245
BseNI ACTGG 4 cut(s) 358, 593, 1325, 1368
BseRI GAGGAG 2 cut(s) 178, 528
BseSI GKGCMC 1 cut(s) 1103
BseXI GCAGC 1 cut(s) 308
Bsh1236I CGCG 2 cut(s) 916, 945
Bsh1285I CGRYCG 1 cut(s) 212
BshFI GGCC 3 cut(s) 234, 372, 427
BshVI ATCGAT 1 cut(s) 707
BsiEI CGRYCG 1 cut(s) 212
BsiHKAI GWGCWC 2 cut(s) 620, 1103
BsiHKCI CYCGRG 1 cut(s) 315
BsiSI CCGG 1 cut(s) 1005
BslFI GGGAC 2 cut(s) 284, 342
BslI CCNNNNNNNGG 8 cut(s) 365, 366, 410, 551, 552, 878, 1093, 1256
BsmAI GTCTC 4 cut(s) 931, 956, 1254, 1333
BsmBI CGTCTC 2 cut(s) 931, 956
BsmFI GGGAC 2 cut(s) 284, 342
BsmI GAATGC 1 cut(s) 1294
BsnI GGCC 3 cut(s) 234, 372, 427
BsoBI CYCGRG 1 cut(s) 315
Bsp1286I GDGCHC 2 cut(s) 620, 1103
Bsp13I TCCGGA 1 cut(s) 1004
Bsp143I GATC 6 cut(s) 303, 555, 981, 1001, 1007, 1355
Bsp68I TCGCGA 2 cut(s) 916, 945
BspACI CCGC 2 cut(s) 208, 720
BspANI GGCC 3 cut(s) 234, 372, 427
BspCNI CTCAG 3 cut(s) 241, 803, 1244
BspDI ATCGAT 1 cut(s) 707
BspEI TCCGGA 1 cut(s) 1004
BspFNI CGCG 2 cut(s) 916, 945
BspHI TCATGA 2 cut(s) 330, 612
BspLI GGNNCC 8 cut(s) 305, 358, 371, 565, 1003, 1058, 1159, 1275
BspMAI CTGCAG 3 cut(s) 298, 689, 1308
BspOI GCTAGC 1 cut(s) 153
BspPI GGATC 8 cut(s) 298, 311, 563, 989, 996, 1009, 1015, 1350
BsrDI GCAATG 2 cut(s) 569, 1381
BsrI ACTGG 4 cut(s) 358, 593, 1325, 1368
BssECI CCNNGG 2 cut(s) 316, 364
BssMI GATC 6 cut(s) 303, 555, 981, 1001, 1007, 1355
BssT1I CCWWGG 1 cut(s) 364
Bst2UI CCWGG 3 cut(s) 374, 576, 873
Bst4CI ACNGT 2 cut(s) 631, 940
Bst6I CTCTTC 1 cut(s) 156
BstC8I GCNNGC 7 cut(s) 151, 280, 439, 443, 918, 922, 1195
BstDEI CTNAG 3 cut(s) 249, 790, 1231
BstENI CCTNNNNNAGG 2 cut(s) 1091, 1254
BstFNI CGCG 2 cut(s) 916, 945
BstKTI GATC 6 cut(s) 306, 558, 984, 1004, 1010, 1358
BstMAI GTCTC 4 cut(s) 931, 956, 1254, 1333
BstMBI GATC 6 cut(s) 303, 555, 981, 1001, 1007, 1355
BstMCI CGRYCG 1 cut(s) 212
BstMWI GCNNNNNNNGC 2 cut(s) 369, 451
BstNI CCWGG 3 cut(s) 374, 576, 873
BstNSI RCATGY 1 cut(s) 924
BstPAI GACNNNNGTC 1 cut(s) 418
BstSCI CCNGG 3 cut(s) 372, 574, 871
BstSFI CTRYAG 4 cut(s) 294, 685, 1254, 1304
BstSLI GKGCMC 1 cut(s) 1103
BstUI CGCG 2 cut(s) 916, 945
BstV1I GCAGC 1 cut(s) 308
BstX2I RGATCY 2 cut(s) 303, 1001
BstXI CCANNNNNNTGG 1 cut(s) 423
BstYI RGATCY 2 cut(s) 303, 1001
Bsu15I ATCGAT 1 cut(s) 707
BsuRI GGCC 3 cut(s) 234, 372, 427
BsuTUI ATCGAT 1 cut(s) 707
BtsI GCAGTG 1 cut(s) 1204
BtsIMutI CAGTG 3 cut(s) 246, 351, 1204
BtuMI TCGCGA 2 cut(s) 916, 945
Cac8I GCNNGC 7 cut(s) 151, 280, 439, 443, 918, 922, 1195
CciI TCATGA 2 cut(s) 330, 612
Cfr13I GGNCC 6 cut(s) 356, 370, 413, 578, 869, 1273
ClaI ATCGAT 1 cut(s) 707
CseI GACGC 1 cut(s) 913
CsiI ACCWGGT 1 cut(s) 871
CviAII CATG 8 cut(s) 331, 582, 613, 878, 921, 990, 1042, 1212
DdeI CTNAG 3 cut(s) 249, 790, 1231
DpnI GATC 6 cut(s) 305, 557, 983, 1003, 1009, 1357
DpnII GATC 6 cut(s) 303, 555, 981, 1001, 1007, 1355
EaeI YGGCCR 2 cut(s) 232, 425
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
EciI GGCGGA 1 cut(s) 709
Ecl136II GAGCTC 1 cut(s) 618
Eco130I CCWWGG 1 cut(s) 364
Eco24I GRGCYC 1 cut(s) 620
Eco47I GGWCC 5 cut(s) 356, 413, 578, 869, 1273
Eco53kI GAGCTC 1 cut(s) 618
Eco57I CTGAAG 3 cut(s) 180, 655, 1098
Eco88I CYCGRG 1 cut(s) 315
EcoICRI GAGCTC 1 cut(s) 618
EcoNI CCTNNNNNAGG 2 cut(s) 1091, 1254
EcoO109I RGGNCCY 1 cut(s) 370
EcoRII CCWGG 3 cut(s) 372, 574, 871
EcoT14I CCWWGG 1 cut(s) 364
EcoT22I ATGCAT 1 cut(s) 1188
EcoT38I GRGCYC 1 cut(s) 620
ErhI CCWWGG 1 cut(s) 364
Esp3I CGTCTC 2 cut(s) 931, 956
FaeI CATG 8 cut(s) 334, 585, 616, 881, 924, 993, 1045, 1215
FalI AAGNNNNNCTT 2 cut(s) 1227, 1259
FaqI GGGAC 2 cut(s) 284, 342
FatI CATG 8 cut(s) 330, 581, 612, 877, 920, 989, 1041, 1211
FblI GTMKAC 1 cut(s) 846
Fnu4HI GCNGC 1 cut(s) 297
FriOI GRGCYC 1 cut(s) 620
Fsp4HI GCNGC 1 cut(s) 297
FspBI CTAG 3 cut(s) 150, 398, 899
GluI GCNGC 1 cut(s) 297
HaeIII GGCC 3 cut(s) 234, 372, 427
HapII CCGG 1 cut(s) 1005
HgaI GACGC 1 cut(s) 913
Hin1II CATG 8 cut(s) 334, 585, 616, 881, 924, 993, 1045, 1215
HpaII CCGG 1 cut(s) 1005
HphI GGTGA 2 cut(s) 818, 1170
Hpy166II GTNNAC 5 cut(s) 66, 847, 869, 1101, 1178
Hpy188I TCNGA 5 cut(s) 674, 738, 1132, 1173, 1234
Hpy188III TCNNGA 9 cut(s) 158, 331, 613, 649, 915, 944, 985, 1005, 1017
Hpy8I GTNNAC 5 cut(s) 66, 847, 869, 1101, 1178
HpyAV CCTTC 1 cut(s) 138
HpyCH4III ACNGT 2 cut(s) 631, 940
HpyF10VI GCNNNNNNNGC 2 cut(s) 369, 451
HpyF3I CTNAG 3 cut(s) 249, 790, 1231
Hsp92II CATG 8 cut(s) 334, 585, 616, 881, 924, 993, 1045, 1215
Kpn2I TCCGGA 1 cut(s) 1004
Kzo9I GATC 6 cut(s) 303, 555, 981, 1001, 1007, 1355
LmnI GCTCC 2 cut(s) 191, 623
Lsp1109I GCAGC 1 cut(s) 308
LweI GCATC 3 cut(s) 454, 1173, 1192
MabI ACCWGGT 1 cut(s) 871
MaeI CTAG 3 cut(s) 150, 398, 899
MaeIII GTNAC 6 cut(s) 18, 378, 584, 806, 1021, 1031
MalI GATC 6 cut(s) 305, 557, 983, 1003, 1009, 1357
MboI GATC 6 cut(s) 303, 555, 981, 1001, 1007, 1355
MboII GAAGA 5 cut(s) 16, 173, 524, 601, 1238
MfeI CAATTG 1 cut(s) 822
MflI RGATCY 2 cut(s) 303, 1001
MhlI GDGCHC 2 cut(s) 620, 1103
MlsI TGGCCA 2 cut(s) 234, 427
MluCI AATT 8 cut(s) 14, 501, 662, 780, 795, 822, 1076, 1317
MluNI TGGCCA 2 cut(s) 234, 427
MlyI GAGTC 3 cut(s) 428, 904, 941
MmeI TCCRAC 4 cut(s) 205, 645, 1265, 1320
Mox20I TGGCCA 2 cut(s) 234, 427
Mph1103I ATGCAT 1 cut(s) 1188
MroI TCCGGA 1 cut(s) 1004
MscI TGGCCA 2 cut(s) 234, 427
MseI TTAA 4 cut(s) 258, 603, 642, 1089
MslI CAYNNNNRTG 1 cut(s) 1040
Msp20I TGGCCA 2 cut(s) 234, 427
MspI CCGG 1 cut(s) 1005
MspR9I CCNGG 3 cut(s) 374, 576, 873
MunI CAATTG 1 cut(s) 822
Mva1269I GAATGC 1 cut(s) 1294
MvaI CCWGG 3 cut(s) 374, 576, 873
MvnI CGCG 2 cut(s) 916, 945
MwoI GCNNNNNNNGC 2 cut(s) 369, 451
NdeII GATC 6 cut(s) 303, 555, 981, 1001, 1007, 1355
NheI GCTAGC 1 cut(s) 149
NlaIII CATG 8 cut(s) 334, 585, 616, 881, 924, 993, 1045, 1215
NlaIV GGNNCC 8 cut(s) 305, 358, 371, 565, 1003, 1058, 1159, 1275
NmuCI GTSAC 2 cut(s) 18, 806
NruI TCGCGA 2 cut(s) 916, 945
NsiI ATGCAT 1 cut(s) 1188
NspI RCATGY 1 cut(s) 924
PaeI GCATGC 1 cut(s) 924
PagI TCATGA 2 cut(s) 330, 612
PctI GAATGC 1 cut(s) 1294
PfeI GAWTC 8 cut(s) 143, 290, 334, 468, 529, 704, 751, 882
PflFI GACNNNGTC 1 cut(s) 873
PflMI CCANNNNNTGG 1 cut(s) 410
PkrI GCNGC 1 cut(s) 298
PleI GAGTC 3 cut(s) 427, 903, 941
PpsI GAGTC 3 cut(s) 427, 903, 941
PshAI GACNNNNGTC 1 cut(s) 418
PsiI TTATAA 1 cut(s) 786
Psp124BI GAGCTC 1 cut(s) 620
Psp6I CCWGG 3 cut(s) 372, 574, 871
PspGI CCWGG 3 cut(s) 372, 574, 871
PspN4I GGNNCC 8 cut(s) 305, 358, 371, 565, 1003, 1058, 1159, 1275
PspPI GGNCC 6 cut(s) 356, 370, 413, 578, 869, 1273
PstI CTGCAG 3 cut(s) 298, 689, 1308
PsuI RGATCY 2 cut(s) 303, 1001
PsyI GACNNNGTC 1 cut(s) 873
RruI TCGCGA 2 cut(s) 916, 945
RseI CAYNNNNRTG 1 cut(s) 1040
SacI GAGCTC 1 cut(s) 620
SaqAI TTAA 4 cut(s) 258, 603, 642, 1089
SatI GCNGC 1 cut(s) 297
Sau3AI GATC 6 cut(s) 303, 555, 981, 1001, 1007, 1355
Sau96I GGNCC 6 cut(s) 356, 370, 413, 578, 869, 1273
SchI GAGTC 3 cut(s) 428, 904, 941
ScrFI CCNGG 3 cut(s) 374, 576, 873
SduI GDGCHC 2 cut(s) 620, 1103
SexAI ACCWGGT 1 cut(s) 871
SfaNI GCATC 3 cut(s) 454, 1173, 1192
SfcI CTRYAG 4 cut(s) 294, 685, 1254, 1304
SinI GGWCC 5 cut(s) 356, 413, 578, 869, 1273
SmiMI CAYNNNNRTG 1 cut(s) 1040
SphI GCATGC 1 cut(s) 924
Sse9I AATT 8 cut(s) 14, 501, 662, 780, 795, 822, 1076, 1317
SsiI CCGC 2 cut(s) 208, 720
SspI AATATT 1 cut(s) 348
SspMI CTAG 3 cut(s) 150, 398, 899
SstI GAGCTC 1 cut(s) 620
StyD4I CCNGG 3 cut(s) 372, 574, 871
StyI CCWWGG 1 cut(s) 364
TaaI ACNGT 2 cut(s) 631, 940
TaqI TCGA 5 cut(s) 201, 707, 730, 1239, 1354
TasI AATT 8 cut(s) 14, 501, 662, 780, 795, 822, 1076, 1317
TfiI GAWTC 8 cut(s) 143, 290, 334, 468, 529, 704, 751, 882
Tru1I TTAA 4 cut(s) 258, 603, 642, 1089
Tru9I TTAA 4 cut(s) 258, 603, 642, 1089
TscAI CASTG 3 cut(s) 253, 358, 1204
TseFI GTSAC 2 cut(s) 18, 806
TseI GCWGC 1 cut(s) 296
Tsp45I GTSAC 2 cut(s) 18, 806
TspDTI ATGAA 4 cut(s) 17, 33, 319, 601
TspGWI ACGGA 1 cut(s) 980
TspRI CASTG 3 cut(s) 253, 358, 1204
Tth111I GACNNNGTC 1 cut(s) 873
Van91I CCANNNNNTGG 1 cut(s) 410
VneI GTGCAC 1 cut(s) 1099
VpaK11BI GGWCC 5 cut(s) 356, 413, 578, 869, 1273
XagI CCTNNNNNAGG 2 cut(s) 1091, 1254
XapI RAATTY 1 cut(s) 780
XceI RCATGY 1 cut(s) 924
XmiI GTMKAC 1 cut(s) 846
XspI CTAG 3 cut(s) 150, 398, 899
Zsp2I ATGCAT 1 cut(s) 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.