pycom17g27760

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0012763
Physical Location & Seq
Forward (+)
25853320 .. 25855160
1841 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g27760.4

Sequence Viewer

Length: 1356 bp
ATGAAGAATTGCAAGTTGTCACTGCTTGTTTTCTGCATTTCTTTCTTTTGCTTCTTATTAGTGTCCACCCAAGCCAGATGGCATAACCATACAAAACACAAGCACTCTCACAAGCAATCCCACATTTCACAACCTCCTTCCTCTGCACCTGAGCCGGCTAGTCGTCCCGAAGAGCCGCCTACCAATCCGGGCCATTCCTCTGATGTTTTCGATGTAAGATCATTTGGTGCTGTAGGAGATGGCCAAACTGATGACACCGCGGCGTTTAAGATGGCATGGGACACAGCCTGCCAAAGTGATGAATCTGCCCCAAGGATCCTTGTTCCCCAAGGTTTCTCGTTCATGATACAATCTACAATATTCACTGGTCCCTGCCTTGCGGGCTTGGTGTTACAGGTTGATGGAACTCTAGTGCCACCTGATGGACCAGAGTCCTGGCCAAAGAACATCAGCAGGCACCAATGGCTGGTCTTTTACCGAGTCAACGAGATGTCGCTGCAAGGCGGCGGTGTAATTGATGGGAGAGGACAGAAATGGTGGAATCTTCCCTGCAAACCCCATAGGGCCCTGAGGTTCTTCATGAGCTCAAACTTGACAGTTCAAGGACTTAGAATTAAGAATAGCCCCCAATTCCACTTTAGATTTGATAATTGTAAAAATGTGCATATAGAATCCATTTCTATATCCGCCCCTGCAAAAAGTCCGAATACAGATGGGATTCACATAGAGAACACAAATGATGTCCAAATTTATAACTCAGTCATTTCTAATGGTGATGATTGTATATCAATTGGATCAGGCTGTTATGATGTAGACATACGGAACATCACTTGTGGACCTGGTCATGGAATCAGCATTGGGAGTCTCGGAAATCACAATTCGCTGGCATGCGTCTCTAACATTACAGTTAGGGACTCGATAATCAAAGGGACGGATAATGGAGTTAGGATCGAGACATGGCAGGGCGGGTCTGGATCGGTATCGGCAGTAACATTCAGTAATATTCACATGGATAATGTTAGAAACCCTATTATGATTGACCAATACTATTGCCTTACCAAAGGGTGCACAAACCAAACTTCAGCAGTGTTCGTATCGGACATACTCTATTCAAACATAAAGGGGACTTATGACGTTCGAAGTGCACCAATGCATTTTGCCTGCAGTGATGCTCTCCCATGCACTAACTTGACACTCTCAGAAGTTGAGCTTCTTCCTGCGGTAGGAGATATGGTATCCGACCCGTATTGTTGGAATGCTTATGGAGAGCTGCAGACGCTAACAATTCCACCAGTCTCTTGCTTGTTGGATGGTGTTCCTCGATCGCTACTGGACACTTACGTTGAGCGTTGCTGA

Protein Analysis

452

Amino Acids

49.43

Weight (kDa)

6.03

Isoelectric Point (pI)

58.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012763)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02460
fragaria_vesca FvH4_1g26650
malus_domestica MD09G1287300.v1.1 MD17G1279500.v1.1
prunus_persica Prupe.3G005900_v2.0.a1 Prupe.6G307200_v2.0.a1
pyrus_communis pycom09g19460 pycom17g27760
rosa_chinensis RchiOBHm_Chr3g0486841
rosa_laevigata RLG00000023055
rosa_multiflora Rmu_sc0005709.1_g000005
rosa_roxburghii Rroxscaffold_6G00395520
rosa_rugosa Rorug03G0227400.1
rosa_samantha Rh3AG277500 Rh3BG312600 Rh3CG311500 Rh3DG308100
rosa_wichuraiana Rw3G024640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 753
AccB1I GGYRCC 1 cut(s) 456
AccB7I CCANNNNNTGG 2 cut(s) 422, 466
AccI GTMKAC 1 cut(s) 813
AccII CGCG 1 cut(s) 260
AciI CCGC 9 cut(s) 176, 258, 260, 380, 504, 507, 687, 966, 1220
AclWI GGATC 5 cut(s) 310, 323, 802, 956, 982
AcoI YGGCCR 2 cut(s) 241, 437
AcsI RAATTY 1 cut(s) 747
AcuI CTGAAG 1 cut(s) 1065
AfiI CCNNNNNNNGG 4 cut(s) 422, 466, 845, 1223
AgsI TTSAA 2 cut(s) 602, 1113
AjnI CCWGG 2 cut(s) 434, 838
AluBI AGCT 3 cut(s) 585, 1210, 1270
AluI AGCT 3 cut(s) 585, 1210, 1270
Alw21I GWGCWC 3 cut(s) 587, 1070, 1147
Alw26I GTCTC 4 cut(s) 869, 898, 947, 1300
Alw44I GTGCAC 2 cut(s) 1066, 1143
AlwI GGATC 5 cut(s) 310, 323, 802, 956, 982
AoxI GGCC 4 cut(s) 190, 241, 437, 564
ApaI GGGCCC 1 cut(s) 568
ApaLI GTGCAC 2 cut(s) 1066, 1143
ApeKI GCWGC 2 cut(s) 496, 1270
ApoI RAATTY 1 cut(s) 747
AspS9I GGNCC 6 cut(s) 190, 368, 425, 564, 565, 836
AsuC2I CCSGG 1 cut(s) 189
AsuHPI GGTGA 1 cut(s) 785
AsuII TTCGAA 1 cut(s) 1138
AvaII GGWCC 3 cut(s) 368, 425, 836
AxyI CCTNAGG 1 cut(s) 569
BaeGI GKGCMC 3 cut(s) 568, 1070, 1147
BalI TGGCCA 2 cut(s) 243, 439
BamHI GGATCC 1 cut(s) 315
BanI GGYRCC 1 cut(s) 456
BanII GRGCYC 2 cut(s) 568, 587
Bbv12I GWGCWC 3 cut(s) 587, 1070, 1147
BbvI GCAGC 2 cut(s) 483, 1257
BccI CCATC 8 cut(s) 72, 233, 265, 395, 416, 512, 707, 1304
BciT130I CCWGG 2 cut(s) 436, 840
BciVI GTATCC 1 cut(s) 1246
BcnI CCSGG 1 cut(s) 189
BcoDI GTCTC 4 cut(s) 869, 898, 947, 1300
BfaI CTAG 2 cut(s) 159, 410
BfmI CTRYAG 3 cut(s) 231, 1162, 1271
BfuI GTATCC 1 cut(s) 1246
BglI GCCNNNNNGGC 1 cut(s) 381
BisI GCNGC 5 cut(s) 176, 261, 497, 505, 1271
BlsI GCNGC 5 cut(s) 177, 262, 498, 506, 1272
Bme1390I CCNGG 3 cut(s) 189, 436, 840
Bme18I GGWCC 3 cut(s) 368, 425, 836
BmgT120I GGNCC 6 cut(s) 190, 368, 425, 564, 565, 836
BmiI GGNNCC 4 cut(s) 317, 370, 458, 566
BmrFI CCNGG 3 cut(s) 189, 436, 840
BmsI GCATC 1 cut(s) 1159
BoxI GACNNNNGTC 1 cut(s) 430
Bpu10I CCTNAGC 1 cut(s) 150
Bpu14I TTCGAA 1 cut(s) 1138
BpuMI CCSGG 1 cut(s) 189
BsaBI GATNNNNATC 1 cut(s) 979
BsaJI CCNNGG 3 cut(s) 258, 311, 328
BsaXI ACNNNNNCTCC 2 cut(s) 1218, 1248
Bsc4I CCNNNNNNNGG 4 cut(s) 422, 466, 845, 1223
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 3 cut(s) 370, 1292, 1335
Bse21I CCTNAGG 1 cut(s) 569
Bse8I GATNNNNATC 1 cut(s) 979
BseBI CCWGG 2 cut(s) 436, 840
BseDI CCNNGG 3 cut(s) 258, 311, 328
BseGI GGATG 1 cut(s) 1315
BseJI GATNNNNATC 1 cut(s) 979
BseLI CCNNNNNNNGG 4 cut(s) 422, 466, 845, 1223
BseMII CTCAG 4 cut(s) 141, 560, 771, 1212
BseNI ACTGG 3 cut(s) 370, 1292, 1335
BseSI GKGCMC 3 cut(s) 568, 1070, 1147
BseXI GCAGC 2 cut(s) 483, 1257
BsgI GTGCAG 1 cut(s) 129
Bsh1236I CGCG 1 cut(s) 260
Bsh1285I CGRYCG 1 cut(s) 1325
BshFI GGCC 4 cut(s) 192, 243, 439, 566
BshNI GGYRCC 1 cut(s) 456
BsiEI CGRYCG 1 cut(s) 1325
BsiHKAI GWGCWC 3 cut(s) 587, 1070, 1147
BsiSI CCGG 2 cut(s) 155, 188
BslFI GGGAC 6 cut(s) 150, 293, 354, 926, 943, 1138
BslI CCNNNNNNNGG 4 cut(s) 422, 466, 845, 1223
BsmAI GTCTC 4 cut(s) 869, 898, 947, 1300
BsmBI CGTCTC 1 cut(s) 898
BsmFI GGGAC 6 cut(s) 150, 293, 354, 926, 943, 1138
BsmI GAATGC 1 cut(s) 1261
BsnI GGCC 4 cut(s) 192, 243, 439, 566
Bsp119I TTCGAA 1 cut(s) 1138
Bsp120I GGGCCC 1 cut(s) 564
Bsp1286I GDGCHC 4 cut(s) 568, 587, 1070, 1147
Bsp143I GATC 6 cut(s) 218, 315, 794, 948, 974, 1322
BspACI CCGC 9 cut(s) 176, 258, 260, 380, 504, 507, 687, 966, 1220
BspANI GGCC 4 cut(s) 192, 243, 439, 566
BspCNI CTCAG 4 cut(s) 142, 561, 770, 1211
BspFNI CGCG 1 cut(s) 260
BspHI TCATGA 2 cut(s) 342, 579
BspLI GGNNCC 4 cut(s) 317, 370, 458, 566
BspMAI CTGCAG 2 cut(s) 1166, 1275
BspPI GGATC 5 cut(s) 310, 323, 802, 956, 982
BspQI GCTCTTC 1 cut(s) 165
BspT104I TTCGAA 1 cut(s) 1138
BspT107I GGYRCC 1 cut(s) 456
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 3 cut(s) 370, 1292, 1335
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 3 cut(s) 258, 311, 328
BssMI GATC 6 cut(s) 218, 315, 794, 948, 974, 1322
BssT1I CCWWGG 2 cut(s) 311, 328
Bst2UI CCWGG 2 cut(s) 436, 840
Bst4CI ACNGT 2 cut(s) 598, 907
Bst6I CTCTTC 1 cut(s) 165
BstBI TTCGAA 1 cut(s) 1138
BstC8I GCNNGC 7 cut(s) 156, 289, 382, 455, 885, 889, 1162
BstDEI CTNAG 5 cut(s) 150, 569, 608, 757, 1198
BstDSI CCRYGG 1 cut(s) 258
BstENI CCTNNNNNAGG 1 cut(s) 1221
BstF5I GGATG 1 cut(s) 1315
BstFNI CGCG 1 cut(s) 260
BstKTI GATC 6 cut(s) 221, 318, 797, 951, 977, 1325
BstMAI GTCTC 4 cut(s) 869, 898, 947, 1300
BstMBI GATC 6 cut(s) 218, 315, 794, 948, 974, 1322
BstMCI CGRYCG 1 cut(s) 1325
BstMWI GCNNNNNNNGC 3 cut(s) 381, 463, 1276
BstNI CCWGG 2 cut(s) 436, 840
BstNSI RCATGY 1 cut(s) 891
BstPAI GACNNNNGTC 1 cut(s) 430
BstSCI CCNGG 3 cut(s) 187, 434, 838
BstSFI CTRYAG 3 cut(s) 231, 1162, 1271
BstSLI GKGCMC 3 cut(s) 568, 1070, 1147
BstUI CGCG 1 cut(s) 260
BstV1I GCAGC 2 cut(s) 483, 1257
BstX2I RGATCY 1 cut(s) 315
BstXI CCANNNNNNTGG 1 cut(s) 435
BstYI RGATCY 1 cut(s) 315
Bsu36I CCTNAGG 1 cut(s) 569
BsuI GTATCC 1 cut(s) 1246
BsuRI GGCC 4 cut(s) 192, 243, 439, 566
BtgI CCRYGG 1 cut(s) 258
BtsCI GGATG 1 cut(s) 1315
BtsI GCAGTG 3 cut(s) 20, 1092, 1171
BtsIMutI CAGTG 4 cut(s) 20, 363, 1092, 1171
Cac8I GCNNGC 7 cut(s) 156, 289, 382, 455, 885, 889, 1162
CciI TCATGA 2 cut(s) 342, 579
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 6 cut(s) 190, 368, 425, 564, 565, 836
Cfr42I CCGCGG 1 cut(s) 261
CseI GACGC 2 cut(s) 880, 1285
CsiI ACCWGGT 1 cut(s) 838
CviAII CATG 8 cut(s) 276, 343, 580, 845, 888, 957, 1009, 1179
DdeI CTNAG 5 cut(s) 150, 569, 608, 757, 1198
DpnI GATC 6 cut(s) 220, 317, 796, 950, 976, 1324
DpnII GATC 6 cut(s) 218, 315, 794, 948, 974, 1322
EaeI YGGCCR 2 cut(s) 241, 437
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
EciI GGCGGA 1 cut(s) 676
Ecl136II GAGCTC 1 cut(s) 585
Eco130I CCWWGG 2 cut(s) 311, 328
Eco24I GRGCYC 2 cut(s) 568, 587
Eco47I GGWCC 3 cut(s) 368, 425, 836
Eco53kI GAGCTC 1 cut(s) 585
Eco57I CTGAAG 1 cut(s) 1065
Eco81I CCTNAGG 1 cut(s) 569
EcoICRI GAGCTC 1 cut(s) 585
EcoNI CCTNNNNNAGG 1 cut(s) 1221
EcoO109I RGGNCCY 2 cut(s) 564, 565
EcoRII CCWGG 2 cut(s) 434, 838
EcoT14I CCWWGG 2 cut(s) 311, 328
EcoT22I ATGCAT 1 cut(s) 1155
EcoT38I GRGCYC 2 cut(s) 568, 587
ErhI CCWWGG 2 cut(s) 311, 328
Esp3I CGTCTC 1 cut(s) 898
FaeI CATG 8 cut(s) 279, 346, 583, 848, 891, 960, 1012, 1182
FalI AAGNNNNNCTT 2 cut(s) 1194, 1226
FaqI GGGAC 6 cut(s) 150, 293, 354, 926, 943, 1138
FatI CATG 8 cut(s) 275, 342, 579, 844, 887, 956, 1008, 1178
FauI CCCGC 2 cut(s) 373, 959
FblI GTMKAC 1 cut(s) 813
Fnu4HI GCNGC 5 cut(s) 176, 261, 497, 505, 1271
FokI GGATG 1 cut(s) 1322
FriOI GRGCYC 2 cut(s) 568, 587
Fsp4HI GCNGC 5 cut(s) 176, 261, 497, 505, 1271
FspBI CTAG 2 cut(s) 159, 410
GluI GCNGC 5 cut(s) 176, 261, 497, 505, 1271
HaeIII GGCC 4 cut(s) 192, 243, 439, 566
HapII CCGG 2 cut(s) 155, 188
HgaI GACGC 2 cut(s) 880, 1285
Hin1II CATG 8 cut(s) 279, 346, 583, 848, 891, 960, 1012, 1182
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HinfI GANTC 9 cut(s) 302, 431, 480, 541, 671, 718, 849, 862, 914
HpaII CCGG 2 cut(s) 155, 188
HphI GGTGA 1 cut(s) 785
Hpy166II GTNNAC 6 cut(s) 66, 484, 814, 836, 1068, 1145
Hpy188I TCNGA 6 cut(s) 202, 705, 869, 1099, 1201, 1240
Hpy188III TCNNGA 5 cut(s) 167, 343, 580, 952, 972
Hpy8I GTNNAC 6 cut(s) 66, 484, 814, 836, 1068, 1145
HpyAV CCTTC 1 cut(s) 147
HpyCH4III ACNGT 2 cut(s) 598, 907
HpyCH4IV ACGT 2 cut(s) 1134, 1341
HpyF10VI GCNNNNNNNGC 3 cut(s) 381, 463, 1276
HpyF3I CTNAG 5 cut(s) 150, 569, 608, 757, 1198
HpySE526I ACGT 2 cut(s) 1134, 1341
Hsp92II CATG 8 cut(s) 279, 346, 583, 848, 891, 960, 1012, 1182
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
KspI CCGCGG 1 cut(s) 261
Kzo9I GATC 6 cut(s) 218, 315, 794, 948, 974, 1322
LguI GCTCTTC 1 cut(s) 165
Lsp1109I GCAGC 2 cut(s) 483, 1257
LweI GCATC 1 cut(s) 1159
MabI ACCWGGT 1 cut(s) 838
MaeI CTAG 2 cut(s) 159, 410
MaeII ACGT 2 cut(s) 1134, 1341
MaeIII GTNAC 3 cut(s) 18, 390, 988
MalI GATC 6 cut(s) 220, 317, 796, 950, 976, 1324
MboI GATC 6 cut(s) 218, 315, 794, 948, 974, 1322
MboII GAAGA 5 cut(s) 16, 182, 536, 568, 1205
MfeI CAATTG 1 cut(s) 789
MflI RGATCY 1 cut(s) 315
MhlI GDGCHC 4 cut(s) 568, 587, 1070, 1147
MlsI TGGCCA 2 cut(s) 243, 439
MluCI AATT 9 cut(s) 7, 513, 612, 629, 649, 747, 789, 877, 1284
MluNI TGGCCA 2 cut(s) 243, 439
MlyI GAGTC 4 cut(s) 440, 489, 871, 908
MmeI TCCRAC 3 cut(s) 1232, 1263, 1287
MnlI CCTC 6 cut(s) 144, 151, 208, 518, 564, 1329
Mox20I TGGCCA 2 cut(s) 243, 439
Mph1103I ATGCAT 1 cut(s) 1155
MroNI GCCGGC 1 cut(s) 154
MscI TGGCCA 2 cut(s) 243, 439
MseI TTAA 2 cut(s) 267, 615
Msp20I TGGCCA 2 cut(s) 243, 439
MspA1I CMGCKG 1 cut(s) 260
MspI CCGG 2 cut(s) 155, 188
MspR9I CCNGG 3 cut(s) 189, 436, 840
MunI CAATTG 1 cut(s) 789
Mva1269I GAATGC 1 cut(s) 1261
MvaI CCWGG 2 cut(s) 436, 840
MvnI CGCG 1 cut(s) 260
MwoI GCNNNNNNNGC 3 cut(s) 381, 463, 1276
NaeI GCCGGC 1 cut(s) 156
NciI CCSGG 1 cut(s) 189
NdeII GATC 6 cut(s) 218, 315, 794, 948, 974, 1322
NgoMIV GCCGGC 1 cut(s) 154
NlaIII CATG 8 cut(s) 279, 346, 583, 848, 891, 960, 1012, 1182
NlaIV GGNNCC 4 cut(s) 317, 370, 458, 566
NmuCI GTSAC 1 cut(s) 18
NsiI ATGCAT 1 cut(s) 1155
NspI RCATGY 1 cut(s) 891
NspV TTCGAA 1 cut(s) 1138
PaeI GCATGC 1 cut(s) 891
PagI TCATGA 2 cut(s) 342, 579
PciSI GCTCTTC 1 cut(s) 165
PctI GAATGC 1 cut(s) 1261
PdiI GCCGGC 1 cut(s) 156
PfeI GAWTC 5 cut(s) 302, 541, 671, 718, 849
PflFI GACNNNGTC 1 cut(s) 840
PflMI CCANNNNNTGG 2 cut(s) 422, 466
PkrI GCNGC 5 cut(s) 177, 262, 498, 506, 1272
Ple19I CGATCG 1 cut(s) 1325
PleI GAGTC 4 cut(s) 439, 488, 870, 908
PpsI GAGTC 4 cut(s) 439, 488, 870, 908
PshAI GACNNNNGTC 1 cut(s) 430
PsiI TTATAA 1 cut(s) 753
Psp124BI GAGCTC 1 cut(s) 587
Psp6I CCWGG 2 cut(s) 434, 838
PspGI CCWGG 2 cut(s) 434, 838
PspN4I GGNNCC 4 cut(s) 317, 370, 458, 566
PspOMI GGGCCC 1 cut(s) 564
PspPI GGNCC 6 cut(s) 190, 368, 425, 564, 565, 836
PstI CTGCAG 2 cut(s) 1166, 1275
PsuI RGATCY 1 cut(s) 315
PsyI GACNNNGTC 1 cut(s) 840
PvuI CGATCG 1 cut(s) 1325
SacI GAGCTC 1 cut(s) 587
SacII CCGCGG 1 cut(s) 261
SapI GCTCTTC 1 cut(s) 165
SaqAI TTAA 2 cut(s) 267, 615
SatI GCNGC 5 cut(s) 176, 261, 497, 505, 1271
Sau3AI GATC 6 cut(s) 218, 315, 794, 948, 974, 1322
Sau96I GGNCC 6 cut(s) 190, 368, 425, 564, 565, 836
SchI GAGTC 4 cut(s) 440, 489, 871, 908
ScrFI CCNGG 3 cut(s) 189, 436, 840
SduI GDGCHC 4 cut(s) 568, 587, 1070, 1147
SexAI ACCWGGT 1 cut(s) 838
SfaNI GCATC 1 cut(s) 1159
SfcI CTRYAG 3 cut(s) 231, 1162, 1271
Sfr303I CCGCGG 1 cut(s) 261
SfuI TTCGAA 1 cut(s) 1138
SgrBI CCGCGG 1 cut(s) 261
SinI GGWCC 3 cut(s) 368, 425, 836
SphI GCATGC 1 cut(s) 891
Sse9I AATT 9 cut(s) 7, 513, 612, 629, 649, 747, 789, 877, 1284
SsiI CCGC 9 cut(s) 176, 258, 260, 380, 504, 507, 687, 966, 1220
SspI AATATT 2 cut(s) 360, 1003
SspMI CTAG 2 cut(s) 159, 410
SstI GAGCTC 1 cut(s) 587
StyD4I CCNGG 3 cut(s) 187, 434, 838
StyI CCWWGG 2 cut(s) 311, 328
TaaI ACNGT 2 cut(s) 598, 907
TaiI ACGT 2 cut(s) 1137, 1344
TaqI TCGA 5 cut(s) 210, 917, 951, 1138, 1321
TasI AATT 9 cut(s) 7, 513, 612, 629, 649, 747, 789, 877, 1284
TauI GCSGC 3 cut(s) 178, 263, 507
TfiI GAWTC 5 cut(s) 302, 541, 671, 718, 849
Tru1I TTAA 2 cut(s) 267, 615
Tru9I TTAA 2 cut(s) 267, 615
TscAI CASTG 4 cut(s) 27, 370, 1092, 1171
TseFI GTSAC 1 cut(s) 18
TseI GCWGC 2 cut(s) 496, 1270
Tsp45I GTSAC 1 cut(s) 18
TspDTI ATGAA 4 cut(s) 17, 315, 331, 568
TspGWI ACGGA 2 cut(s) 835, 947
TspRI CASTG 4 cut(s) 27, 370, 1092, 1171
Tth111I GACNNNGTC 1 cut(s) 840
Van91I CCANNNNNTGG 2 cut(s) 422, 466
VneI GTGCAC 2 cut(s) 1066, 1143
VpaK11BI GGWCC 3 cut(s) 368, 425, 836
XagI CCTNNNNNAGG 1 cut(s) 1221
XapI RAATTY 1 cut(s) 747
XceI RCATGY 1 cut(s) 891
XmiI GTMKAC 1 cut(s) 813
XspI CTAG 2 cut(s) 159, 410
Zsp2I ATGCAT 1 cut(s) 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.