MD17G1279500.v1.1

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012763
Physical Location & Seq
Forward (+)
33997321 .. 33999501
2181 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1279500.v1.1.491

Sequence Viewer

Length: 1401 bp
ATGAAGAATTGCAAGTTGTCACTGCTTGTTTTCTGCATTTCATTCTTTTGCTTCTTATTAATGTCCACCCAAGCCAGATGGCATAACCATACAAAACACAAGCACTCTCACAAGCAATCCAACATTTCACAACCTCCTTCCTCCTCACCTGAGCCTGCTAGTCCTCCCGAAGAGCCGCCTACCAATCCGCACCATTCCTCCGGTGCTTTCGATGTAAGATCATTTGGTGCTGTTGGAGATGGCCAAACTGATGACACCGCGGCGTTTAAGATGGCATGGGACACAGCCTGCCAAAGTGATGAATCTGCTGCAAGGATCCTTGTTCCCCAAGGTTTCTCATTCATGATACAATCTACAATATTCACTGGTCCCTGCCTTGGGGGCTTGGTGTTACAGGTTGATGGAACTCTAGTACCACCTGATGGACCAGAGTCCTGGCCAAAGAACATCAGCAGGCACCAATGGCTGGTCTTTTACCGAGTCAATGAGATGTCACTGCAAGGCGGCGGTGTAATTGATGGGAGAGGAGAGAAATGGTGGAATCTTCCCTGCAAACCCCATAGGGGGATAAATGGAACCACATTGCCAGGGCCATGCGATAGCCCAATTGCCCTGAGGTTCTTCATGAGCTCCAACTTGACAGTTCAAGGACTTAGAATTAAGAATAGTCCCAAATTCCACTTCAGATTTGATAATTGTAAGAATGTGCATATAGAATCCATTTCTATATCCGCCCCTGCAAAAAGTCCGAATACAGATGGGATTCACATAGAGAACACAAATGATGTCCAAATTTATAACTCAGTCATTTCTAATGGTGATGATTGTATATCAATTGGATCAGGCTGTTATGATGTAGACATACGGAACATCACTTGTGGACCTGGTCACGGAATCAGCATTGGGAGTCTCGGAAATCACAATTCGCGGGCATGCGTCTCTAACATTACAGTTAGGGACTCGATAATCAAAGGGACGGATAATGGAGTTAGGATCAAGACATGGCAGGGCGGGTCTGGATCGGTATCTGCAGTAACATTCAGTAATATTCACATGGATAATGTTAGAAACCCTATTATGATTGACCAATACTATTGCCTTACCAAAGGGTGCACAAACCAAACTTCAGCAGTGTTCGTATCTGACATACTCTATTCAAACATAAAGGGGACTTATGACGTTCGGAGTTCACCAATGCATTTTGCCTGCAGTGATGCTCTCCCATGCACTAACTTGACACTCTCAGAAGTTGAGCTCCTTCCTGCGGTAGGAGACAAGGTATCCGACCCGTATTGTTGGAATGCTTATGGAGAGCTGCAGACGCTGACAATTCCACCAGTCTCTTGCTTGTTGGATGGTGTTCCTCGATCCCTACTGGACAAGTACGTTGAGCGTTGCTGA

Protein Analysis

467

Amino Acids

50.86

Weight (kDa)

6.45

Isoelectric Point (pI)

59.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 100 - 420 4.7e-93 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012763)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02460
fragaria_vesca FvH4_1g26650
malus_domestica MD09G1287300.v1.1 MD17G1279500.v1.1
prunus_persica Prupe.3G005900_v2.0.a1 Prupe.6G307200_v2.0.a1
pyrus_communis pycom09g19460 pycom17g27760
rosa_chinensis RchiOBHm_Chr3g0486841
rosa_laevigata RLG00000023055
rosa_multiflora Rmu_sc0005709.1_g000005
rosa_roxburghii Rroxscaffold_6G00395520
rosa_rugosa Rorug03G0227400.1
rosa_samantha Rh3AG277500 Rh3BG312600 Rh3CG311500 Rh3DG308100
rosa_wichuraiana Rw3G024640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 798
AccB1I GGYRCC 1 cut(s) 456
AccB7I CCANNNNNTGG 2 cut(s) 422, 466
AccI GTMKAC 1 cut(s) 858
AccII CGCG 2 cut(s) 260, 928
AclWI GGATC 6 cut(s) 310, 323, 847, 1001, 1027, 1362
AcoI YGGCCR 2 cut(s) 241, 437
AcsI RAATTY 2 cut(s) 674, 792
AcuI CTGAAG 2 cut(s) 667, 1110
AfaI GTAC 2 cut(s) 414, 1385
AfiI CCNNNNNNNGG 9 cut(s) 377, 378, 422, 466, 563, 564, 890, 1264, 1268
AgsI TTSAA 2 cut(s) 647, 1158
AjnI CCWGG 3 cut(s) 434, 586, 883
AjuI GAANNNNNNNTTGG 2 cut(s) 665, 697
AluBI AGCT 3 cut(s) 630, 1255, 1315
AluI AGCT 3 cut(s) 630, 1255, 1315
Alw21I GWGCWC 3 cut(s) 632, 1115, 1257
Alw26I GTCTC 4 cut(s) 914, 943, 1266, 1345
Alw44I GTGCAC 1 cut(s) 1111
AlwI GGATC 6 cut(s) 310, 323, 847, 1001, 1027, 1362
AlwNI CAGNNNCTG 1 cut(s) 1324
AoxI GGCC 3 cut(s) 241, 437, 590
ApaLI GTGCAC 1 cut(s) 1111
ApeKI GCWGC 2 cut(s) 308, 1315
ApoI RAATTY 2 cut(s) 674, 792
AseI ATTAAT 1 cut(s) 59
AspS9I GGNCC 4 cut(s) 368, 425, 590, 881
AsuHPI GGTGA 3 cut(s) 138, 830, 1182
AvaII GGWCC 3 cut(s) 368, 425, 881
AxyI CCTNAGG 1 cut(s) 614
BaeGI GKGCMC 1 cut(s) 1115
BaeI ACNNNNGTAYC 2 cut(s) 396, 429
BalI TGGCCA 2 cut(s) 243, 439
BamHI GGATCC 1 cut(s) 315
BanI GGYRCC 1 cut(s) 456
BanII GRGCYC 2 cut(s) 632, 1257
Bbv12I GWGCWC 3 cut(s) 632, 1115, 1257
BbvI GCAGC 2 cut(s) 295, 1302
BccI CCATC 8 cut(s) 72, 233, 265, 395, 416, 512, 752, 1349
BciT130I CCWGG 3 cut(s) 436, 588, 885
BciVI GTATCC 1 cut(s) 1291
BcoDI GTCTC 4 cut(s) 914, 943, 1266, 1345
BfaI CTAG 2 cut(s) 159, 410
BfmI CTRYAG 3 cut(s) 1029, 1207, 1316
BfuI GTATCC 1 cut(s) 1291
BglI GCCNNNNNGGC 1 cut(s) 381
BisI GCNGC 5 cut(s) 176, 261, 309, 505, 1316
BlsI GCNGC 5 cut(s) 177, 262, 310, 506, 1317
Bme1390I CCNGG 3 cut(s) 436, 588, 885
Bme18I GGWCC 3 cut(s) 368, 425, 881
BmgT120I GGNCC 4 cut(s) 368, 425, 590, 881
BmiI GGNNCC 4 cut(s) 317, 370, 458, 577
BmrFI CCNGG 3 cut(s) 436, 588, 885
BmsI GCATC 1 cut(s) 1204
BoxI GACNNNNGTC 1 cut(s) 430
Bpu10I CCTNAGC 1 cut(s) 150
BsaBI GATNNNNATC 1 cut(s) 1024
BsaJI CCNNGG 4 cut(s) 258, 328, 376, 587
BsaWI WCCGGW 1 cut(s) 200
BsaXI ACNNNNNCTCC 4 cut(s) 182, 212, 1263, 1293
Bsc4I CCNNNNNNNGG 9 cut(s) 377, 378, 422, 466, 563, 564, 890, 1264, 1268
Bse1I ACTGG 3 cut(s) 370, 1337, 1380
Bse21I CCTNAGG 1 cut(s) 614
Bse3DI GCAATG 1 cut(s) 581
Bse8I GATNNNNATC 1 cut(s) 1024
BseBI CCWGG 3 cut(s) 436, 588, 885
BseDI CCNNGG 4 cut(s) 258, 328, 376, 587
BseGI GGATG 1 cut(s) 1360
BseJI GATNNNNATC 1 cut(s) 1024
BseLI CCNNNNNNNGG 9 cut(s) 377, 378, 422, 466, 563, 564, 890, 1264, 1268
BseMI GCAATG 1 cut(s) 581
BseMII CTCAG 4 cut(s) 141, 605, 816, 1257
BseNI ACTGG 3 cut(s) 370, 1337, 1380
BseRI GAGGAG 2 cut(s) 133, 540
BseSI GKGCMC 1 cut(s) 1115
BseXI GCAGC 2 cut(s) 295, 1302
Bsh1236I CGCG 2 cut(s) 260, 928
BshFI GGCC 3 cut(s) 243, 439, 592
BshNI GGYRCC 1 cut(s) 456
BsiHKAI GWGCWC 3 cut(s) 632, 1115, 1257
BsiSI CCGG 1 cut(s) 201
BslFI GGGAC 6 cut(s) 293, 354, 654, 971, 988, 1183
BslI CCNNNNNNNGG 9 cut(s) 377, 378, 422, 466, 563, 564, 890, 1264, 1268
BsmAI GTCTC 4 cut(s) 914, 943, 1266, 1345
BsmBI CGTCTC 1 cut(s) 943
BsmFI GGGAC 6 cut(s) 293, 354, 654, 971, 988, 1183
BsmI GAATGC 1 cut(s) 1306
BsnI GGCC 3 cut(s) 243, 439, 592
Bsp1286I GDGCHC 3 cut(s) 632, 1115, 1257
Bsp143I GATC 6 cut(s) 218, 315, 839, 993, 1019, 1367
BspANI GGCC 3 cut(s) 243, 439, 592
BspCNI CTCAG 4 cut(s) 142, 606, 815, 1256
BspFNI CGCG 2 cut(s) 260, 928
BspHI TCATGA 2 cut(s) 342, 624
BspLI GGNNCC 4 cut(s) 317, 370, 458, 577
BspMAI CTGCAG 3 cut(s) 1033, 1211, 1320
BspPI GGATC 6 cut(s) 310, 323, 847, 1001, 1027, 1362
BspQI GCTCTTC 1 cut(s) 165
BspT107I GGYRCC 1 cut(s) 456
BsrDI GCAATG 1 cut(s) 581
BsrI ACTGG 3 cut(s) 370, 1337, 1380
BssECI CCNNGG 4 cut(s) 258, 328, 376, 587
BssMI GATC 6 cut(s) 218, 315, 839, 993, 1019, 1367
BssT1I CCWWGG 2 cut(s) 328, 376
Bst2UI CCWGG 3 cut(s) 436, 588, 885
Bst4CI ACNGT 2 cut(s) 643, 952
Bst6I CTCTTC 1 cut(s) 165
BstC8I GCNNGC 6 cut(s) 156, 289, 455, 930, 934, 1207
BstDEI CTNAG 5 cut(s) 150, 614, 653, 802, 1243
BstDSI CCRYGG 1 cut(s) 258
BstENI CCTNNNNNAGG 1 cut(s) 1266
BstF5I GGATG 1 cut(s) 1360
BstFNI CGCG 2 cut(s) 260, 928
BstKTI GATC 6 cut(s) 221, 318, 842, 996, 1022, 1370
BstMAI GTCTC 4 cut(s) 914, 943, 1266, 1345
BstMBI GATC 6 cut(s) 218, 315, 839, 993, 1019, 1367
BstMWI GCNNNNNNNGC 3 cut(s) 381, 463, 1321
BstNI CCWGG 3 cut(s) 436, 588, 885
BstNSI RCATGY 1 cut(s) 936
BstPAI GACNNNNGTC 1 cut(s) 430
BstSCI CCNGG 3 cut(s) 434, 586, 883
BstSFI CTRYAG 3 cut(s) 1029, 1207, 1316
BstSLI GKGCMC 1 cut(s) 1115
BstUI CGCG 2 cut(s) 260, 928
BstV1I GCAGC 2 cut(s) 295, 1302
BstX2I RGATCY 1 cut(s) 315
BstXI CCANNNNNNTGG 1 cut(s) 435
BstYI RGATCY 1 cut(s) 315
Bsu36I CCTNAGG 1 cut(s) 614
BsuI GTATCC 1 cut(s) 1291
BsuRI GGCC 3 cut(s) 243, 439, 592
BtgI CCRYGG 1 cut(s) 258
BtsCI GGATG 1 cut(s) 1360
BtsI GCAGTG 4 cut(s) 20, 494, 1137, 1216
BtsIMutI CAGTG 5 cut(s) 20, 363, 494, 1137, 1216
Cac8I GCNNGC 6 cut(s) 156, 289, 455, 930, 934, 1207
CaiI CAGNNNCTG 1 cut(s) 1324
CciI TCATGA 2 cut(s) 342, 624
Cfr13I GGNCC 4 cut(s) 368, 425, 590, 881
Cfr42I CCGCGG 1 cut(s) 261
CseI GACGC 2 cut(s) 925, 1330
CsiI ACCWGGT 1 cut(s) 883
Csp6I GTAC 2 cut(s) 413, 1384
CviAII CATG 8 cut(s) 276, 343, 594, 625, 933, 1002, 1054, 1224
CviQI GTAC 2 cut(s) 413, 1384
DdeI CTNAG 5 cut(s) 150, 614, 653, 802, 1243
DpnI GATC 6 cut(s) 220, 317, 841, 995, 1021, 1369
DpnII GATC 6 cut(s) 218, 315, 839, 993, 1019, 1367
EaeI YGGCCR 2 cut(s) 241, 437
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
EciI GGCGGA 1 cut(s) 721
Ecl136II GAGCTC 2 cut(s) 630, 1255
Eco130I CCWWGG 2 cut(s) 328, 376
Eco24I GRGCYC 2 cut(s) 632, 1257
Eco47I GGWCC 3 cut(s) 368, 425, 881
Eco53kI GAGCTC 2 cut(s) 630, 1255
Eco57I CTGAAG 2 cut(s) 667, 1110
Eco81I CCTNAGG 1 cut(s) 614
EcoICRI GAGCTC 2 cut(s) 630, 1255
EcoNI CCTNNNNNAGG 1 cut(s) 1266
EcoRII CCWGG 3 cut(s) 434, 586, 883
EcoT14I CCWWGG 2 cut(s) 328, 376
EcoT22I ATGCAT 1 cut(s) 1200
EcoT38I GRGCYC 2 cut(s) 632, 1257
ErhI CCWWGG 2 cut(s) 328, 376
Esp3I CGTCTC 1 cut(s) 943
FaeI CATG 8 cut(s) 279, 346, 597, 628, 936, 1005, 1057, 1227
FaqI GGGAC 6 cut(s) 293, 354, 654, 971, 988, 1183
FatI CATG 8 cut(s) 275, 342, 593, 624, 932, 1001, 1053, 1223
FauI CCCGC 2 cut(s) 921, 1004
FblI GTMKAC 1 cut(s) 858
Fnu4HI GCNGC 5 cut(s) 176, 261, 309, 505, 1316
FokI GGATG 1 cut(s) 1367
FriOI GRGCYC 2 cut(s) 632, 1257
Fsp4HI GCNGC 5 cut(s) 176, 261, 309, 505, 1316
FspBI CTAG 2 cut(s) 159, 410
GluI GCNGC 5 cut(s) 176, 261, 309, 505, 1316
HaeIII GGCC 3 cut(s) 243, 439, 592
HapII CCGG 1 cut(s) 201
HgaI GACGC 2 cut(s) 925, 1330
Hin1II CATG 8 cut(s) 279, 346, 597, 628, 936, 1005, 1057, 1227
HinfI GANTC 9 cut(s) 302, 431, 480, 541, 716, 763, 894, 907, 959
HpaII CCGG 1 cut(s) 201
HphI GGTGA 3 cut(s) 138, 830, 1182
Hpy166II GTNNAC 5 cut(s) 66, 859, 881, 1113, 1190
Hpy188I TCNGA 7 cut(s) 686, 750, 914, 1144, 1185, 1246, 1285
Hpy188III TCNNGA 5 cut(s) 167, 343, 625, 997, 1017
Hpy8I GTNNAC 5 cut(s) 66, 859, 881, 1113, 1190
HpyAV CCTTC 2 cut(s) 147, 1268
HpyCH4III ACNGT 2 cut(s) 643, 952
HpyCH4IV ACGT 2 cut(s) 1179, 1386
HpyF10VI GCNNNNNNNGC 3 cut(s) 381, 463, 1321
HpyF3I CTNAG 5 cut(s) 150, 614, 653, 802, 1243
HpySE526I ACGT 2 cut(s) 1179, 1386
Hsp92II CATG 8 cut(s) 279, 346, 597, 628, 936, 1005, 1057, 1227
KspI CCGCGG 1 cut(s) 261
Kzo9I GATC 6 cut(s) 218, 315, 839, 993, 1019, 1367
LguI GCTCTTC 1 cut(s) 165
LmnI GCTCC 2 cut(s) 635, 1260
Lsp1109I GCAGC 2 cut(s) 295, 1302
LweI GCATC 1 cut(s) 1204
MabI ACCWGGT 1 cut(s) 883
MaeI CTAG 2 cut(s) 159, 410
MaeII ACGT 2 cut(s) 1179, 1386
MaeIII GTNAC 5 cut(s) 18, 390, 492, 887, 1033
MalI GATC 6 cut(s) 220, 317, 841, 995, 1021, 1369
MboI GATC 6 cut(s) 218, 315, 839, 993, 1019, 1367
MboII GAAGA 4 cut(s) 16, 182, 536, 613
MfeI CAATTG 2 cut(s) 606, 834
MflI RGATCY 1 cut(s) 315
MhlI GDGCHC 3 cut(s) 632, 1115, 1257
MlsI TGGCCA 2 cut(s) 243, 439
MluNI TGGCCA 2 cut(s) 243, 439
MlyI GAGTC 4 cut(s) 440, 489, 916, 953
MmeI TCCRAC 6 cut(s) 144, 214, 657, 1277, 1308, 1332
MnlI CCTC 8 cut(s) 144, 151, 154, 174, 208, 518, 609, 1374
Mox20I TGGCCA 2 cut(s) 243, 439
Mph1103I ATGCAT 1 cut(s) 1200
MscI TGGCCA 2 cut(s) 243, 439
MseI TTAA 3 cut(s) 59, 267, 660
Msp20I TGGCCA 2 cut(s) 243, 439
MspA1I CMGCKG 1 cut(s) 260
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 3 cut(s) 436, 588, 885
MunI CAATTG 2 cut(s) 606, 834
Mva1269I GAATGC 1 cut(s) 1306
MvaI CCWGG 3 cut(s) 436, 588, 885
MvnI CGCG 2 cut(s) 260, 928
MwoI GCNNNNNNNGC 3 cut(s) 381, 463, 1321
NdeII GATC 6 cut(s) 218, 315, 839, 993, 1019, 1367
NlaIII CATG 8 cut(s) 279, 346, 597, 628, 936, 1005, 1057, 1227
NlaIV GGNNCC 4 cut(s) 317, 370, 458, 577
NmuCI GTSAC 3 cut(s) 18, 492, 887
NsiI ATGCAT 1 cut(s) 1200
NspI RCATGY 1 cut(s) 936
PaeI GCATGC 1 cut(s) 936
PagI TCATGA 2 cut(s) 342, 624
PciSI GCTCTTC 1 cut(s) 165
PctI GAATGC 1 cut(s) 1306
PfeI GAWTC 5 cut(s) 302, 541, 716, 763, 894
PflFI GACNNNGTC 1 cut(s) 885
PflMI CCANNNNNTGG 2 cut(s) 422, 466
PkrI GCNGC 5 cut(s) 177, 262, 310, 506, 1317
PleI GAGTC 4 cut(s) 439, 488, 915, 953
PpsI GAGTC 4 cut(s) 439, 488, 915, 953
PshAI GACNNNNGTC 1 cut(s) 430
PshBI ATTAAT 1 cut(s) 59
PsiI TTATAA 1 cut(s) 798
Psp124BI GAGCTC 2 cut(s) 632, 1257
Psp6I CCWGG 3 cut(s) 434, 586, 883
PspGI CCWGG 3 cut(s) 434, 586, 883
PspN4I GGNNCC 4 cut(s) 317, 370, 458, 577
PspPI GGNCC 4 cut(s) 368, 425, 590, 881
PstI CTGCAG 3 cut(s) 1033, 1211, 1320
PstNI CAGNNNCTG 1 cut(s) 1324
PsuI RGATCY 1 cut(s) 315
PsyI GACNNNGTC 1 cut(s) 885
RsaI GTAC 2 cut(s) 414, 1385
RsaNI GTAC 2 cut(s) 413, 1384
SacI GAGCTC 2 cut(s) 632, 1257
SacII CCGCGG 1 cut(s) 261
SapI GCTCTTC 1 cut(s) 165
SaqAI TTAA 3 cut(s) 59, 267, 660
SatI GCNGC 5 cut(s) 176, 261, 309, 505, 1316
Sau3AI GATC 6 cut(s) 218, 315, 839, 993, 1019, 1367
Sau96I GGNCC 4 cut(s) 368, 425, 590, 881
SchI GAGTC 4 cut(s) 440, 489, 916, 953
ScrFI CCNGG 3 cut(s) 436, 588, 885
SduI GDGCHC 3 cut(s) 632, 1115, 1257
SexAI ACCWGGT 1 cut(s) 883
SfaNI GCATC 1 cut(s) 1204
SfcI CTRYAG 3 cut(s) 1029, 1207, 1316
Sfr303I CCGCGG 1 cut(s) 261
SgrBI CCGCGG 1 cut(s) 261
SinI GGWCC 3 cut(s) 368, 425, 881
SphI GCATGC 1 cut(s) 936
SspI AATATT 2 cut(s) 360, 1048
SspMI CTAG 2 cut(s) 159, 410
SstI GAGCTC 2 cut(s) 632, 1257
StyD4I CCNGG 3 cut(s) 434, 586, 883
StyI CCWWGG 2 cut(s) 328, 376
TaaI ACNGT 2 cut(s) 643, 952
TaiI ACGT 2 cut(s) 1182, 1389
TaqI TCGA 3 cut(s) 210, 962, 1366
TauI GCSGC 3 cut(s) 178, 263, 507
TfiI GAWTC 5 cut(s) 302, 541, 716, 763, 894
Tru1I TTAA 3 cut(s) 59, 267, 660
Tru9I TTAA 3 cut(s) 59, 267, 660
TscAI CASTG 5 cut(s) 27, 370, 501, 1137, 1216
TseFI GTSAC 3 cut(s) 18, 492, 887
TseI GCWGC 2 cut(s) 308, 1315
Tsp45I GTSAC 3 cut(s) 18, 492, 887
TspDTI ATGAA 5 cut(s) 17, 30, 315, 331, 613
TspGWI ACGGA 3 cut(s) 880, 906, 992
TspRI CASTG 5 cut(s) 27, 370, 501, 1137, 1216
Tth111I GACNNNGTC 1 cut(s) 885
Van91I CCANNNNNTGG 2 cut(s) 422, 466
VneI GTGCAC 1 cut(s) 1111
VpaK11BI GGWCC 3 cut(s) 368, 425, 881
VspI ATTAAT 1 cut(s) 59
XagI CCTNNNNNAGG 1 cut(s) 1266
XapI RAATTY 2 cut(s) 674, 792
XceI RCATGY 1 cut(s) 936
XmiI GTMKAC 1 cut(s) 858
XspI CTAG 2 cut(s) 159, 410
Zsp2I ATGCAT 1 cut(s) 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.