FvH4_1g28360

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004406
Physical Location & Seq
Reverse (-)
20245145 .. 20266228
21084 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g28360.t12

Sequence Viewer

Length: 3210 bp
ATGGATTTTTCACTTCAAGGAAAGAAACAGATTTTGCAGACACATGCTCGTAAAAATTTGTCAAGGGGAGCCCACAAAGATCTGTGGTTTGCTGTGCAACAAGGGTCATTGGCCGATGTGGATTCAGCACTATCCTTTCTGAAGAAGAGTGGTAGTAATATTAATTCAAGAAACATATTTGGTCTCACTCCTCTTCATATTGCAACTTGGAGGAATCACATTCCCATTGTTAGGAGGCTTCTTGCAGCTGGAGCTGACCCAGATGCCAGGGATGGGGAATCAGGATGGAGTAGCCTTCACAGAGCTATGCATTTTGGTCATCTTGCTGTGGCAAGCATACTTCTCCAGTGTGGTGCATCTATCACACTGGAGGACTCTAAATACCGAACACCTGTTGATCTGATATCTGGGCCTGTGTTGCAAGTTCTTGGTAGCGGCCAGAATTCAGAGGTTTTCAGCTGGGGAAGTGGTGCAAACTATCAGCTTGGAACTGGAAACGCACACATACAAAAGTTGCCATGCAAGGTTGATGCTCTTCATGATTCTCTAATCAGGTCTGTTTCTGCCGCTAAATTTCATAGTGTAGCAGTTACTGCTCGAGGAGAAGTGTATACTTGGGGATTTGGAAGAGGAGGACGACTTGGGCACCCTGATTTTGATATACACAGCGGTCAAGCTGCGGTTATCACTCCTCGGCAGGTAACATCTGGTCTAGGGTCCCGTCGGGTTAAGGCAGTTGCTGCAGCTAAACATCACACTGTTGTTGCTACAGATGGTGGGGAGGTGTTCACCTGGGGATCCAACCGAGAGGGTCAGCTTGGCTACACTTCTGTGGATACTCAACCTACACCTCGTAGAGTGAGTTCCCTCAGGTCAAAGATTGTCGCTGTTGCTGCAGCAAACAAGCACACTGCTGTGGTTTCTGATAATGGTGAAGTTTTCACATGGGGTTGCAACAGAGAGGGCCAGCTTGGCTATGGTACATCCAACTCTGCATCAAATTACACTTCTAGACTGGTTGAGTACTTGAAAGGCAAGGTTTTTATGGGGGTTGCAACAGCAAAATATCACACACTTGTCTTAGGAGTTGATGGAGAGGTATACACTTGGGGTCATCGACTTGTTACGCCTAAACGGGTTGTTGTCACCAGAAATTTGAAAAAGGGTGGAAATTCCACTTTGAAGTTTCATCGCAAAGAACGGCTGCATGTGGTTTCGATAGCTGCTGGGATGGTACATAGCATGGCTCTCACAGATGACGGTGCTTTGTTTTATTGGGTTTCTTCAGATCCTGATCTTAGATGCCAACAGCTTTACTCGCTGTGCGGAAGAAATTTGGTGAACATTTCTGCAGGGAAGTACTGGACTGCCTCTGTTACAGCTACAGGTGATGTTTATATGTGGGATGGGAAAAATGGCAAGGATAAGCCACTAGTTGCAACTCGGTTACATGGTACAAAGCGGGCTACTTCAGTTTCAGTTGGTGAAACACATTTGTTGATTATTGGTTCTCTCTATCATCCTGCCTATACTTCCAATGTGGTGAAGGATCCACAGAAGTCAGTTATCAGTGAGGAGCTAGAAGAGATTGATGAAGATCTTATGTTTAATGACATAGACTCAGAGAATCCTTCACCTATTATACAAGATGATGACTCCAGCAAGAATGCCGTACCAAGTTTAAAAAGTATTTGTGAAAAAGTGGCTGCAGAGAAATTAGTGGAGCCACGAAATGCTATCCAGCTGCTTGAAATTGCAGATTCACTCATGGCAGATGATCTAAGGAAGTATTGTGAGGACATTGCTATACGAAACCTTGACTATATTTTTACCGTATCATCACAAGCTATAGCTGGAGCCTCACCAGATATTTTAGGTAACCTCGAAAAATCATTGGATTTGAAGTCATCTGAACCATGGAGCTACCGTCGTCTCCCAACTCCGACAGCTACTTTTCCTGCTGTTATTTATAGCGAGGAGGAAGATAGTGAGAGTGAGGTTCAAAGAACACGTGACAGCCGTACCAAGCAATCTACCTCCAATATTGAAATGCACCAGAGACCAGATTCCTTTTTACAACCCAAAGATGATCCAAGTCGAGGTATTGGCAAGGAAGTTCGAGCCTTGCGGAAAAAGCTGCAGCAAATTGAGATGCTCGAGGAAAAACAATCCAATGGGTATCTTCTTGATGACCAGCAAATTAAAAAGCTTAAAACAAGGTCAGCTTTAGAGAATTCACTTGCTGATCTCGGGGTTCCAGTTGAGACTACAGAGTTGAAGGAATCATCTTCAGTTCTCCCAGATGGAAAAGGAAACAAAAAGGTTGAGCTTTCAAGAAAACTAGGGAGAAAGAACAAGCAGATTACAACGCAAGTGGCTCGGCTTCCTGCAAGTGAAATAGAACCAAACCCAATAAAAGGTTCCTTGAATAGTGAACTCTGCTCAGATAATAAGGAGGAAGATTCCACGGTTGGTGAGATTATGACCAGCCAAACGACGACAGAGTCGGCTTTATTTTTCCCGAAGGAGAAGTTAGATTCAACAAAGAACCATTTGTCACCAACAGTTTCCAAGAAGAAGAGTAAAAAGGGTGGGCTTTCAATGTTCTTGAGTGGTGCACTTGATGACTCCCCCAAGTATATTGCTCCTCCACCAACCCCAAAAAGTGAGGGTCCTGCATGGGGTGGGGCTAAAATTTCCAAAGGATTTGCGTCTCTCCGTGACATTCAAGATGAGGAAATTAAAATCAAGGTGCATCAATCAACTAGGAATAAAGATCCCTTGGAAGACCCTGTCATCGCTAAAGGTGATGGGAAGATCCTTTTGAGTTCATTTTTGCCTTCTAAGCCAATTCCGGTAGGTTCAGTTGTCTCGACTTCTGCTTCACTGGCAAATGAAGGAGAAAGATATACACCTCCCTGGACTGCTTCAGGAACTCCTCCTCTTGCTCGTCCATCTCTTAGGGACATCCAAATGCAGCAGAAAGGAAAACAACAGCAAAATCTGTCTCACAGTCCAAAGACAAAAACAGCTGGATTCTCAGTGACTAATGGTCTGTGTTCGCCATTGGACTCTTCTGGAGTGAACCGCTGGTTCAAACCGGAAGTGGATGCAGCCTCATCTATTCGTTCAATCCAGATCGAGGAAAAGGCCATGAAGGACCTTAGGCGCTTCTACAACAGTGTTAAGGTTGTCAAGAACACTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_4 Ank_5 RCC1

Protein Analysis

1070

Amino Acids

116.75

Weight (kDa)

9.11

Isoelectric Point (pI)

41.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 28 - 125 4.6e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 43 - 81 3.4e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 52 - 102 1.3e-07 Ankyrin repeats (many copies)
Ank PF00023 61 - 91 1.2e-07 Ankyrin repeat
Ank_4 PF13637 72 - 115 1.4e-06 Ankyrin repeats (many copies)
WD40_RLD PF25390 150 - 371 1.1e-49 RCC1-like domain
RCC1 PF00415 150 - 197 5.6e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 201 - 255 5.9e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 243 - 272 4.6e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 260 - 307 4.9e-13 Regulator of chromosome condensation (RCC1) repeat
WD40_RLD PF25390 281 - 500 1.2e-30 RCC1-like domain
RCC1_2 PF13540 294 - 323 3.8e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 310 - 361 8.9e-09 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2503
Acc36I ACCTGC 1 cut(s) 688
AccB1I GGYRCC 1 cut(s) 645
AccI GTMKAC 2 cut(s) 611, 1101
AciI CCGC 8 cut(s) 435, 567, 669, 680, 1326, 1462, 2128, 3088
AclWI GGATC 8 cut(s) 792, 805, 1283, 1544, 1557, 2084, 2771, 2812
AcoI YGGCCR 2 cut(s) 111, 436
AcsI RAATTY 8 cut(s) 55, 442, 572, 1153, 1171, 1333, 2233, 2694
AcuI CTGAAG 5 cut(s) 161, 1269, 1455, 2274, 2913
AcvI CACGTG 1 cut(s) 2012
AfaI GTAC 7 cut(s) 982, 1025, 1236, 1361, 1456, 1674, 2023
AfiI CCNNNNNNNGG 6 cut(s) 231, 273, 2099, 2417, 2681, 3142
AflIII ACRYGT 1 cut(s) 2009
AhdI GACNNNNNGTC 1 cut(s) 3051
AhlI ACTAGT 1 cut(s) 1432
AjnI CCWGG 3 cut(s) 266, 791, 2918
AjuI GAANNNNNNNTTGG 2 cut(s) 2404, 2436
AleI CACNNNNGTG 2 cut(s) 830, 914
Alw21I GWGCWC 1 cut(s) 2620
Alw26I GTCTC 7 cut(s) 188, 1937, 2053, 2258, 2718, 2875, 3012
Alw44I GTGCAC 1 cut(s) 2616
AlwI GGATC 8 cut(s) 792, 805, 1283, 1544, 1557, 2084, 2771, 2812
AlwNI CAGNNNCTG 3 cut(s) 593, 740, 1292
Ama87I CYCGRG 3 cut(s) 597, 2156, 2249
AoxI GGCC 5 cut(s) 111, 410, 436, 964, 3150
ApaLI GTGCAC 1 cut(s) 2616
ApoI RAATTY 8 cut(s) 55, 442, 572, 1153, 1171, 1333, 2233, 2694
AseI ATTAAT 1 cut(s) 162
AspLEI GCGC 1 cut(s) 3171
AspS9I GGNCC 5 cut(s) 410, 717, 964, 2672, 3160
AvaI CYCGRG 3 cut(s) 597, 2156, 2249
AvaII GGWCC 3 cut(s) 717, 2672, 3160
AxyI CCTNAGG 2 cut(s) 869, 3164
BaeGI GKGCMC 2 cut(s) 648, 2620
BaeI ACNNNNGTAYC 2 cut(s) 2005, 2038
BamHI GGATCC 2 cut(s) 797, 1549
BanI GGYRCC 1 cut(s) 645
BanII GRGCYC 1 cut(s) 73
BbrPI CACGTG 1 cut(s) 2012
BbsI GAAGAC 1 cut(s) 2793
Bbv12I GWGCWC 1 cut(s) 2620
BccI CCATC 9 cut(s) 266, 279, 767, 1085, 1225, 1400, 2297, 2804, 2962
BceAI ACGGC 3 cut(s) 1217, 1655, 2004
BcgI CGANNNNNNTGC 2 cut(s) 2360, 2394
BciT130I CCWGG 3 cut(s) 268, 793, 2920
BciVI GTATCC 1 cut(s) 829
BcoDI GTCTC 7 cut(s) 188, 1937, 2053, 2258, 2718, 2875, 3012
BcuI ACTAGT 1 cut(s) 1432
BfaI CTAG 6 cut(s) 713, 1011, 1433, 1580, 2342, 2766
BfmI CTRYAG 9 cut(s) 741, 768, 894, 1350, 1383, 1707, 1848, 2138, 2268
BfoI RGCGCY 1 cut(s) 3172
BfuAI ACCTGC 1 cut(s) 688
BfuI GTATCC 1 cut(s) 829
BglI GCCNNNNNGGC 1 cut(s) 972
BglII AGATCT 2 cut(s) 79, 1597
BmcAI AGTACT 2 cut(s) 1025, 1361
Bme1390I CCNGG 3 cut(s) 268, 793, 2920
Bme18I GGWCC 3 cut(s) 717, 2672, 3160
BmeRI GACNNNNNGTC 1 cut(s) 3051
BmeT110I CYCGRG 3 cut(s) 597, 2156, 2249
BmgT120I GGNCC 5 cut(s) 410, 717, 964, 2672, 3160
BmrFI CCNGG 3 cut(s) 268, 793, 2920
BmsI GCATC 8 cut(s) 253, 365, 520, 1004, 1292, 2142, 2764, 3100
BpiI GAAGAC 1 cut(s) 2793
BpmI CTGGAG 6 cut(s) 270, 329, 389, 1642, 1875, 3099
BpuEI CTTGAG 1 cut(s) 2629
BsaAI YACGTR 1 cut(s) 2012
BsaBI GATNNNNATC 2 cut(s) 1293, 1596
BsaI GGTCTC 2 cut(s) 188, 2053
BsaJI CCNNGG 7 cut(s) 267, 692, 792, 1916, 2466, 2781, 2918
BsaWI WCCGGW 2 cut(s) 2854, 3100
BsaXI ACNNNNNCTCC 2 cut(s) 1912, 1942
Bsc4I CCNNNNNNNGG 6 cut(s) 231, 273, 2099, 2417, 2681, 3142
Bse1I ACTGG 7 cut(s) 346, 372, 496, 1020, 1367, 2258, 2892
Bse21I CCTNAGG 2 cut(s) 869, 3164
Bse3DI GCAATG 1 cut(s) 1800
Bse8I GATNNNNATC 2 cut(s) 1293, 1596
BseBI CCWGG 3 cut(s) 268, 793, 2920
BseDI CCNNGG 7 cut(s) 267, 692, 792, 1916, 2466, 2781, 2918
BseGI GGATG 8 cut(s) 277, 290, 983, 1236, 1411, 1519, 2967, 3115
BseJI GATNNNNATC 2 cut(s) 1293, 1596
BseLI CCNNNNNNNGG 6 cut(s) 231, 273, 2099, 2417, 2681, 3142
BseMI GCAATG 1 cut(s) 1800
BseMII CTCAG 4 cut(s) 883, 1635, 2457, 3054
BseNI ACTGG 7 cut(s) 346, 372, 496, 1020, 1367, 2258, 2892
BseRI GAGGAG 9 cut(s) 180, 615, 645, 681, 1589, 1991, 2637, 2928, 2931
BseSI GKGCMC 2 cut(s) 648, 2620
BseYI CCCAGC 2 cut(s) 459, 1226
BshFI GGCC 5 cut(s) 113, 412, 438, 966, 3152
BshNI GGYRCC 1 cut(s) 645
BsiHKAI GWGCWC 1 cut(s) 2620
BsiHKCI CYCGRG 3 cut(s) 597, 2156, 2249
BsiSI CCGG 2 cut(s) 2855, 3101
BslFI GGGAC 2 cut(s) 703, 2978
BslI CCNNNNNNNGG 6 cut(s) 231, 273, 2099, 2417, 2681, 3142
BsmAI GTCTC 7 cut(s) 188, 1937, 2053, 2258, 2718, 2875, 3012
BsmBI CGTCTC 2 cut(s) 1937, 2718
BsmFI GGGAC 2 cut(s) 703, 2978
BsmI GAATGC 1 cut(s) 1672
BsnI GGCC 5 cut(s) 113, 412, 438, 966, 3152
Bso31I GGTCTC 2 cut(s) 188, 2053
BsoBI CYCGRG 3 cut(s) 597, 2156, 2249
Bsp1286I GDGCHC 3 cut(s) 73, 648, 2620
Bsp19I CCATGG 1 cut(s) 1916
BspACI CCGC 8 cut(s) 435, 567, 669, 680, 1326, 1462, 2128, 3088
BspANI GGCC 5 cut(s) 113, 412, 438, 966, 3152
BspCNI CTCAG 4 cut(s) 882, 1634, 2456, 3053
BspHI TCATGA 1 cut(s) 538
BspMAI CTGCAG 5 cut(s) 745, 898, 1354, 1711, 2142
BspMI ACCTGC 1 cut(s) 688
BspPI GGATC 8 cut(s) 792, 805, 1283, 1544, 1557, 2084, 2771, 2812
BspQI GCTCTTC 1 cut(s) 540
BspT107I GGYRCC 1 cut(s) 645
BspTNI GGTCTC 2 cut(s) 188, 2053
BsrDI GCAATG 1 cut(s) 1800
BsrI ACTGG 7 cut(s) 346, 372, 496, 1020, 1367, 2258, 2892
BssECI CCNNGG 7 cut(s) 267, 692, 792, 1916, 2466, 2781, 2918
BssNAI GTATAC 2 cut(s) 612, 1102
BssT1I CCWWGG 2 cut(s) 1916, 2781
Bst1107I GTATAC 2 cut(s) 612, 1102
Bst2UI CCWGG 3 cut(s) 268, 793, 2920
Bst4CI ACNGT 8 cut(s) 760, 1262, 1834, 1928, 2470, 2566, 3014, 3182
Bst6I CTCTTC 7 cut(s) 140, 198, 540, 622, 1578, 2573, 3079
BstAPI GCANNNNNTGC 2 cut(s) 593, 740
BstBAI YACGTR 1 cut(s) 2012
BstC8I GCNNGC 3 cut(s) 334, 968, 1464
BstDSI CCRYGG 2 cut(s) 1916, 2466
BstEII GGTNACC 1 cut(s) 1877
BstF5I GGATG 8 cut(s) 277, 290, 983, 1236, 1411, 1519, 2967, 3115
BstH2I RGCGCY 1 cut(s) 3172
BstHHI GCGC 1 cut(s) 3171
BstMAI GTCTC 7 cut(s) 188, 1937, 2053, 2258, 2718, 2875, 3012
BstNI CCWGG 3 cut(s) 268, 793, 2920
BstNSI RCATGY 2 cut(s) 47, 1211
BstPI GGTNACC 1 cut(s) 1877
BstSCI CCNGG 3 cut(s) 266, 791, 2918
BstSFI CTRYAG 9 cut(s) 741, 768, 894, 1350, 1383, 1707, 1848, 2138, 2268
BstSLI GKGCMC 2 cut(s) 648, 2620
BstV2I GAAGAC 1 cut(s) 2793
BstX2I RGATCY 7 cut(s) 79, 797, 1288, 1549, 1597, 2776, 2817
BstYI RGATCY 7 cut(s) 79, 797, 1288, 1549, 1597, 2776, 2817
BstZ17I GTATAC 2 cut(s) 612, 1102
Bsu36I CCTNAGG 2 cut(s) 869, 3164
BsuI GTATCC 1 cut(s) 829
BsuRI GGCC 5 cut(s) 113, 412, 438, 966, 3152
BtgI CCRYGG 2 cut(s) 1916, 2466
BtgZI GCGATG 2 cut(s) 1175, 2782
BtsCI GGATG 8 cut(s) 277, 290, 983, 1236, 1411, 1519, 2967, 3115
BtsI GCAGTG 1 cut(s) 909
BtsIMutI CAGTG 8 cut(s) 353, 365, 756, 909, 1576, 2885, 3048, 3187
BveI ACCTGC 1 cut(s) 688
Cac8I GCNNGC 3 cut(s) 334, 968, 1464
CaiI CAGNNNCTG 3 cut(s) 593, 740, 1292
CciI TCATGA 1 cut(s) 538
CfoI GCGC 1 cut(s) 3171
Cfr13I GGNCC 5 cut(s) 410, 717, 964, 2672, 3160
CseI GACGC 1 cut(s) 2700
Csp6I GTAC 7 cut(s) 981, 1024, 1235, 1360, 1455, 1673, 2022
CspCI CAANNNNNGTGG 2 cut(s) 2355, 2390
CviQI GTAC 7 cut(s) 981, 1024, 1235, 1360, 1455, 1673, 2022
DraI TTTAAA 1 cut(s) 1683
DrdI GACNNNNNNGTC 1 cut(s) 2503
DriI GACNNNNNGTC 1 cut(s) 3051
DseDI GACNNNNNNGTC 1 cut(s) 2503
EaeI YGGCCR 2 cut(s) 111, 436
Eam1104I CTCTTC 7 cut(s) 140, 198, 540, 622, 1578, 2573, 3079
Eam1105I GACNNNNNGTC 1 cut(s) 3051
EarI CTCTTC 7 cut(s) 140, 198, 540, 622, 1578, 2573, 3079
Eco130I CCWWGG 2 cut(s) 1916, 2781
Eco24I GRGCYC 1 cut(s) 73
Eco31I GGTCTC 2 cut(s) 188, 2053
Eco32I GATATC 1 cut(s) 405
Eco47I GGWCC 3 cut(s) 717, 2672, 3160
Eco57I CTGAAG 5 cut(s) 161, 1269, 1455, 2274, 2913
Eco72I CACGTG 1 cut(s) 2012
Eco81I CCTNAGG 2 cut(s) 869, 3164
Eco88I CYCGRG 3 cut(s) 597, 2156, 2249
Eco91I GGTNACC 1 cut(s) 1877
EcoO109I RGGNCCY 3 cut(s) 717, 2672, 3160
EcoO65I GGTNACC 1 cut(s) 1877
EcoRI GAATTC 2 cut(s) 442, 2233
EcoRII CCWGG 3 cut(s) 266, 791, 2918
EcoRV GATATC 1 cut(s) 405
EcoT14I CCWWGG 2 cut(s) 1916, 2781
EcoT22I ATGCAT 1 cut(s) 312
EcoT38I GRGCYC 1 cut(s) 73
ErhI CCWWGG 2 cut(s) 1916, 2781
Esp3I CGTCTC 2 cut(s) 1937, 2718
FalI AAGNNNNNCTT 2 cut(s) 2209, 2241
FaqI GGGAC 2 cut(s) 703, 2978
FauI CCCGC 1 cut(s) 1455
FblI GTMKAC 2 cut(s) 611, 1101
FokI GGATG 8 cut(s) 284, 297, 970, 1243, 1418, 1506, 2954, 3122
FriOI GRGCYC 1 cut(s) 73
FspBI CTAG 6 cut(s) 713, 1011, 1433, 1580, 2342, 2766
GlaI GCGC 1 cut(s) 3170
GsaI CCCAGC 2 cut(s) 463, 1230
GsuI CTGGAG 6 cut(s) 270, 329, 389, 1642, 1875, 3099
HaeII RGCGCY 1 cut(s) 3172
HaeIII GGCC 5 cut(s) 113, 412, 438, 966, 3152
HapII CCGG 2 cut(s) 2855, 3101
HgaI GACGC 1 cut(s) 2700
HhaI GCGC 1 cut(s) 3171
Hin6I GCGC 1 cut(s) 3169
HinP1I GCGC 1 cut(s) 3169
HindIII AAGCTT 1 cut(s) 2207
HpaII CCGG 2 cut(s) 2855, 3101
Hpy166II GTNNAC 7 cut(s) 612, 789, 1102, 1342, 2435, 2618, 3085
Hpy188I TCNGA 9 cut(s) 141, 402, 448, 925, 1288, 1624, 1912, 1944, 2446
Hpy8I GTNNAC 7 cut(s) 612, 789, 1102, 1342, 2435, 2618, 3085
Hpy99I CGWCG 3 cut(s) 726, 1932, 2500
HpyAV CCTTC 8 cut(s) 305, 1540, 1641, 2272, 2518, 2850, 2891, 3151
HpyCH4III ACNGT 8 cut(s) 760, 1262, 1834, 1928, 2470, 2566, 3014, 3182
HpyCH4IV ACGT 1 cut(s) 2011
HpySE526I ACGT 1 cut(s) 2011
HspAI GCGC 1 cut(s) 3169
KflI GGGWCCC 1 cut(s) 717
LguI GCTCTTC 1 cut(s) 540
LmnI GCTCC 7 cut(s) 68, 251, 1576, 1723, 1856, 1920, 2650
LweI GCATC 8 cut(s) 253, 365, 520, 1004, 1292, 2142, 2764, 3100
MaeI CTAG 6 cut(s) 713, 1011, 1433, 1580, 2342, 2766
MaeII ACGT 1 cut(s) 2011
MflI RGATCY 7 cut(s) 79, 797, 1288, 1549, 1597, 2776, 2817
MhlI GDGCHC 3 cut(s) 73, 648, 2620
MlyI GAGTC 6 cut(s) 368, 1613, 1649, 2513, 2621, 3065
MmeI TCCRAC 3 cut(s) 825, 1011, 1967
Mph1103I ATGCAT 1 cut(s) 312
MseI TTAA 9 cut(s) 162, 729, 1608, 1682, 2202, 2211, 2742, 3186, 3208
MslI CAYNNNNRTG 3 cut(s) 830, 914, 2972
MspA1I CMGCKG 6 cut(s) 248, 459, 669, 1744, 3032, 3090
MspI CCGG 2 cut(s) 2855, 3101
MspR9I CCNGG 3 cut(s) 268, 793, 2920
Mva1269I GAATGC 1 cut(s) 1672
MvaI CCWGG 3 cut(s) 268, 793, 2920
NcoI CCATGG 1 cut(s) 1916
NmeAIII GCCGAG 2 cut(s) 673, 2359
NmuCI GTSAC 5 cut(s) 1144, 2012, 2556, 2720, 3043
NsiI ATGCAT 1 cut(s) 312
NspI RCATGY 2 cut(s) 47, 1211
OliI CACNNNNGTG 2 cut(s) 830, 914
PaeR7I CTCGAG 2 cut(s) 597, 2156
PagI TCATGA 1 cut(s) 538
PciSI GCTCTTC 1 cut(s) 540
PcsI WCGNNNNNNNCGW 1 cut(s) 2017
PctI GAATGC 1 cut(s) 1672
PflFI GACNNNGTC 2 cut(s) 2503, 2792
PleI GAGTC 6 cut(s) 368, 1613, 1649, 2512, 2621, 3065
PmaCI CACGTG 1 cut(s) 2012
PmlI CACGTG 1 cut(s) 2012
PpsI GAGTC 6 cut(s) 368, 1613, 1649, 2512, 2621, 3065
Ppu21I YACGTR 1 cut(s) 2012
PpuMI RGGWCCY 3 cut(s) 717, 2672, 3160
PshBI ATTAAT 1 cut(s) 162
Psp5II RGGWCCY 3 cut(s) 717, 2672, 3160
Psp6I CCWGG 3 cut(s) 266, 791, 2918
PspCI CACGTG 1 cut(s) 2012
PspEI GGTNACC 1 cut(s) 1877
PspFI CCCAGC 2 cut(s) 459, 1226
PspGI CCWGG 3 cut(s) 266, 791, 2918
PspPI GGNCC 5 cut(s) 410, 717, 964, 2672, 3160
PspPPI RGGWCCY 3 cut(s) 717, 2672, 3160
PspXI VCTCGAGB 2 cut(s) 597, 2156
PsrI GAACNNNNNNTAC 2 cut(s) 847, 879
PstI CTGCAG 5 cut(s) 745, 898, 1354, 1711, 2142
PstNI CAGNNNCTG 3 cut(s) 593, 740, 1292
PsuI RGATCY 7 cut(s) 79, 797, 1288, 1549, 1597, 2776, 2817
PsyI GACNNNGTC 2 cut(s) 2503, 2792
PvuII CAGCTG 4 cut(s) 248, 459, 1744, 3032
RsaI GTAC 7 cut(s) 982, 1025, 1236, 1361, 1456, 1674, 2023
RsaNI GTAC 7 cut(s) 981, 1024, 1235, 1360, 1455, 1673, 2022
RseI CAYNNNNRTG 3 cut(s) 830, 914, 2972
SapI GCTCTTC 1 cut(s) 540
SaqAI TTAA 9 cut(s) 162, 729, 1608, 1682, 2202, 2211, 2742, 3186, 3208
Sau96I GGNCC 5 cut(s) 410, 717, 964, 2672, 3160
ScaI AGTACT 2 cut(s) 1025, 1361
SchI GAGTC 6 cut(s) 368, 1613, 1649, 2513, 2621, 3065
ScrFI CCNGG 3 cut(s) 268, 793, 2920
SduI GDGCHC 3 cut(s) 73, 648, 2620
SfaNI GCATC 8 cut(s) 253, 365, 520, 1004, 1292, 2142, 2764, 3100
SfcI CTRYAG 9 cut(s) 741, 768, 894, 1350, 1383, 1707, 1848, 2138, 2268
Sfr274I CTCGAG 2 cut(s) 597, 2156
SinI GGWCC 3 cut(s) 717, 2672, 3160
SlaI CTCGAG 2 cut(s) 597, 2156
SmiMI CAYNNNNRTG 3 cut(s) 830, 914, 2972
SmlI CTYRAG 3 cut(s) 597, 2156, 2608
SmoI CTYRAG 3 cut(s) 597, 2156, 2608
SpeI ACTAGT 1 cut(s) 1432
SsiI CCGC 8 cut(s) 435, 567, 669, 680, 1326, 1462, 2128, 3088
SspI AATATT 2 cut(s) 160, 2044
SspMI CTAG 6 cut(s) 713, 1011, 1433, 1580, 2342, 2766
StyD4I CCNGG 3 cut(s) 266, 791, 2918
StyI CCWWGG 2 cut(s) 1916, 2781
TaaI ACNGT 8 cut(s) 760, 1262, 1834, 1928, 2470, 2566, 3014, 3182
TaiI ACGT 1 cut(s) 2014
TaqI TCGA 9 cut(s) 598, 1117, 1217, 1884, 2098, 2119, 2157, 2873, 3141
TatI WGTACW 2 cut(s) 1023, 1359
TauI GCSGC 2 cut(s) 438, 569
Tru1I TTAA 9 cut(s) 162, 729, 1608, 1682, 2202, 2211, 2742, 3186, 3208
Tru9I TTAA 9 cut(s) 162, 729, 1608, 1682, 2202, 2211, 2742, 3186, 3208
TscAI CASTG 8 cut(s) 353, 372, 763, 916, 1576, 2892, 3048, 3187
TseFI GTSAC 5 cut(s) 1144, 2012, 2556, 2720, 3043
Tsp45I GTSAC 5 cut(s) 1144, 2012, 2556, 2720, 3043
TspDTI ATGAA 8 cut(s) 185, 527, 566, 1178, 1608, 2820, 2910, 3170
TspGWI ACGGA 1 cut(s) 2708
TspRI CASTG 8 cut(s) 353, 372, 763, 916, 1576, 2892, 3048, 3187
Tth111I GACNNNGTC 2 cut(s) 2503, 2792
VneI GTGCAC 1 cut(s) 2616
VpaK11BI GGWCC 3 cut(s) 717, 2672, 3160
VspI ATTAAT 1 cut(s) 162
XapI RAATTY 8 cut(s) 55, 442, 572, 1153, 1171, 1333, 2233, 2694
XbaI TCTAGA 1 cut(s) 1010
XceI RCATGY 2 cut(s) 47, 1211
XcmI CCANNNNNNNNNTGG 1 cut(s) 974
XhoI CTCGAG 2 cut(s) 597, 2156
XmiI GTMKAC 2 cut(s) 611, 1101
XspI CTAG 6 cut(s) 713, 1011, 1433, 1580, 2342, 2766
ZrmI AGTACT 2 cut(s) 1025, 1361
Zsp2I ATGCAT 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.