MD12G1006900.v1.1

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004406
Physical Location & Seq
Reverse (-)
780583 .. 788639
8057 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1006900.v1.1.491

Sequence Viewer

Length: 3219 bp
ATGGAAGCCTCACCTCAAGGACAAAAGCCTCCTCGCAAATTTCTGTCGACTGGAACCCAGAAAGATCTTTGCTTTGTTGTGCGAGAAGGATCATTGGCTGATGTGGATTCAGCACTAGGTTTACTGAAGAAGAGTGGTGGTGATATAAATTCAAGAAACATTTTTGGTCTCACTCCTCTTCATATTGCAACATGGAGAAACCACATTCCCATTGTTAGGAGGCTTCTTGCGGCTGGCGCTGACCCAGATATTAGGGATGGGGAATCTGGATGGAGTAGCCTTCACAGAGCTCTCCATTTTGGTCATCTTGCAGTGGCAAGTATACTTCTCCAGTCTGGTGCTTCTATCACACTAGAGGATTCTAAATCTCGAACACCTATTGATCTCCTCTCTGGGCCTGTCTTGCAAGCTCTTCAAAATGGACACAACTCTGTTACGACGGAGGTTTATAGTTGGGGAAGTGGTACAAACTATCAACTTGGAACTGGAAATGCACACATACAAAAGTTACCATGCAAGGTTGATGTTCTTCGTTGTTCTTTAATCAAGTTGGTTTCTGCTGCTAAGTTCCATAGTGTGGCAGTCACTTCTCGAGGAGAAGTCTACACTTGGGGGTTTGGAAGAGGAGGACGACTTGGTCACCCTGATTTTGATATACACAGCGGTCAAGCTGCAGTTATCACACCGCGTCACGTGACATTTGGTATAGGGTCCCGTCGGGTGAAGGCAATTGCGGCAGCTAAACATCACACCGTTATTGCTACGGAGGGTGGGGAAGTGTTCACCTGGGGGTCCAATAGAGAGGGTCAGCTTGGCTACACTTCCGTGGATACCCAACCTACACCTCGTAGAGTGAGTTCCCTTAGGTCAAAAGTTGTTGCAGTTGCTGCCGCCAACAAGCACACTGCTGTTGTTTCAAACGGTGGTGAAGTTTTCACATGGGGGTGCAATAGGGAAGGTCAGCTTGGCTATGGTACATCTAACTCTGCTTCAAACTACACCCCAAGATTAGTTGAATCCTTGAAAGGCAAGGTTTTTAAAGGGGTTGCAGCAGCAAAATATCACACAATTATATTGGGAGTTGATGGGGAGGTATACACTTGGGGTCATCGCATTGTCACGCCAAAACGTGTTTTAATTGCAAGAAACTTGAAAAAGAGTGGAACTACCACTTTGAAGTTTCATCGCAAGGAACGGCTTCATGTGGTTTCTATAGCTGCAGGGATGGTATATAGCACAGCTCTGACAGATGATGGCGCATTATTTTATTGGGTGTCATCAGATCCGGATTTCAGAAGCCAGCAGCTGTATTCATTGAGTGGAAGAAGTATGGTGAACATATCTGCAGGGAAATACTGGACTGCCGCAGTTACAGCTACAGGTGATGTTTACATGTGGGATGGGAAGAATGGTAAGGATAAGCCACCAGTTGCGACTCGGTTACATGGTACGAAGCGGGCTACTTCAGTTTCTGTAGGCGAAACACACTTGTTGATTATTGGCTCCCTTTATCACCCTGCCTACACTTCCAATAACCCTCCGAAGCAGAAGTCAAATGCCAAAGATGAGCTAGAAGAGCTTGATGAAGATCTTATGTTCAATGACATGGAGTCTGACAATGTTTTACCTACTATCCAAAATGATGATGCTGGGAAGAAGCCCATACCGAGCTTGAAAAGTCTTTGTCAAAAAGTGGCTGCAGAGAATCTAGTGGAGCCACGAAATACTATTCAACTGCTCGAAATTGCCGATTCACTTGTGGCAGATGATTTGCGGAAGTACTGTGAGGAGATTGCCATACGCAACCTTGACTATATTTTTACGGTATCATCACAAGCTATTGCTAGTGCTTCACCAGATGTTCTAGCTAACCTTGAAAACGTATTGGACCTAAGGTCATCTGAACCATGGAGTTACCGTTGTTTCCCAACTCCTACAGCTACTTTTCCTGCTATTATTTATAGTGAAGAGGAAGATAGTAATGGTGAGGTTCAAAGGACGCGTGACGGCCATACAGAGCAATCCATCTGGAAAAATGAAATCCACCAAAGAGCAGATTCCTTTCTTCAACCCAAAGATGATCCAAATCATGGTATTGAAAAGCAAGTTCGAGCCTTGCGGAAAAAGCTGCAGCAGATTGAGATGCTTGAAGCAAAACAGTCTAGGGGGGAAGTTCTTGATGACCAGCAAATTACAAAGCTCCAAACGAGGTCAGCTCTAGAGAGCTCGCTGGCTGAGCTTGGTGTTCCAGTTGAGACACCACAGTTGAAGACACCATCGTCAGTTTTGCTAGATGGAAAAGTGAACAAAAAGGTTGAGCCATCAAAAAAACAGAAGAAAAGGAACAAAAAGATGGCAACACAAGTGGAAATAGGGTTGTGTTTTTCGGGAAATGAAGGAGAACTAAACCATGCTAATGGTTTCTTGAATACTGAAACCTCTCAAACCTCAAAGGATAAGGAGGAGGATGCCATGATCGAAGGGATTGCGACAAGCCAAACCAATCAAGAGTCAGCTTTGTGTGTTAAGAAGGACAATTTAAACCTAACAAATAACAAGTGTTCATCACCAATAGTGTCCAAGAAGAAGAATAAAAAGGGTGGGCTTTCTATGTTCTTGAGTGGCGCTCTTGATGATGCCCCCAAGCATGTTGCACCCCCTCCACCATCCCCAAAAAGTGAAGGCCCGGCATGGGGTGGTGCTAAATTTTCCAAAGGGTTTGCATCCCTTCGCGAGATTCAGGATGAGCAAAGCAGAACCAAGGAGAGTCGGTCAACTGGGAACAAAAGTCACCCAGAAGATCCTTTCGATGATAGAAGCGATGGAAAGATTCGTCTGAGTTCTTTTTTACCTTCCAAGCCAATACCATTGGTTTCAACCCATACTCCACTGGCATCTGATGGAGACAGAGGTACACCTTCTTGGACTGCTTCAGGAACTCCTCCCCTTGTTTCTCGCCCATCTCTGAGGGACATCCAAATGCAGCAGGGCAAGCAACACCAGAGTGTATCCCACAGTCCAAAGACAAAAACCACCGGATTCTCAGTCACAAGTGGTCAGGGATCGCCACTGGATACTTCTGGAGTGAACCGGTGGTTCAAACCGGAGGTAGATCAACCGTCACCAATTCGGTCAATTCAGATCGAGGAAAAGGCTATGAAGGAGCTTAGGCGCTTCTACGACAGTGTTAAGATTGTCAAGAAGCCGATGGTAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_4 Ank_5 RCC1

Protein Analysis

1073

Amino Acids

117.11

Weight (kDa)

9.17

Isoelectric Point (pI)

40.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 41 - 120 1.7e-12 Ankyrin repeats (3 copies)
Ank_5 PF13857 46 - 97 5.4e-08 Ankyrin repeats (many copies)
Ank PF00023 56 - 86 1.3e-07 Ankyrin repeat
Ank_4 PF13637 67 - 110 3.8e-07 Ankyrin repeats (many copies)
WD40_RLD PF25390 148 - 369 2e-49 RCC1-like domain
RCC1 PF00415 148 - 195 1.3e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 199 - 253 9.4e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 241 - 270 5.3e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 258 - 305 3.6e-13 Regulator of chromosome condensation (RCC1) repeat
WD40_RLD PF25390 279 - 498 2.8e-32 RCC1-like domain
RCC1_2 PF13540 292 - 321 5.1e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 309 - 359 8.4e-13 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 636, 2278
AccB7I CCANNNNNTGG 2 cut(s) 577, 2090
AccI GTMKAC 4 cut(s) 47, 322, 603, 1095
AccII CGCG 3 cut(s) 688, 2002, 2733
AccIII TCCGGA 1 cut(s) 1285
AciI CCGC 9 cut(s) 230, 663, 686, 734, 891, 1365, 1456, 1774, 2119
AclWI GGATC 5 cut(s) 97, 1277, 2075, 2795, 3070
AcoI YGGCCR 1 cut(s) 2008
AcsI RAATTY 3 cut(s) 38, 148, 2705
AcuI CTGAAG 3 cut(s) 146, 1449, 2916
AcvI CACGTG 1 cut(s) 694
AfaI GTAC 5 cut(s) 466, 976, 1450, 1781, 2914
AfiI CCNNNNNNNGG 5 cut(s) 216, 577, 2090, 2692, 2693
AflIII ACRYGT 3 cut(s) 1129, 1392, 2000
AgeI ACCGGT 1 cut(s) 3090
AhdI GACNNNNNGTC 2 cut(s) 1609, 1894
AjnI CCWGG 1 cut(s) 785
AjuI GAANNNNNNNTTGG 2 cut(s) 948, 980
AleI CACNNNNGTG 2 cut(s) 824, 3003
Alw21I GWGCWC 2 cut(s) 292, 2228
Alw26I GTCTC 3 cut(s) 173, 2249, 2898
AlwI GGATC 5 cut(s) 97, 1277, 2075, 2795, 3070
AlwNI CAGNNNCTG 3 cut(s) 887, 1306, 1472
Ama87I CYCGRG 1 cut(s) 591
Aor13HI TCCGGA 1 cut(s) 1285
AoxI GGCC 3 cut(s) 395, 2008, 2683
ApoI RAATTY 3 cut(s) 38, 148, 2705
ArsI GACNNNNNNTTYG 2 cut(s) 1535, 1567
AsiGI ACCGGT 1 cut(s) 3090
Asp700I GAANNNNTTC 1 cut(s) 1197
AspLEI GCGC 4 cut(s) 239, 1259, 2627, 3174
AspS9I GGNCC 5 cut(s) 395, 711, 792, 1888, 2684
AsuC2I CCSGG 1 cut(s) 2687
AvaI CYCGRG 1 cut(s) 591
AvaII GGWCC 3 cut(s) 711, 792, 1888
AxyI CCTNAGG 2 cut(s) 863, 1892
BanII GRGCYC 2 cut(s) 292, 2228
BbrPI CACGTG 1 cut(s) 694
BbsI GAAGAC 1 cut(s) 2276
Bbv12I GWGCWC 2 cut(s) 292, 2228
BceAI ACGGC 2 cut(s) 1211, 2023
BcgI CGANNNNNNTGC 2 cut(s) 2468, 2502
BciT130I CCWGG 1 cut(s) 787
BciVI GTATCC 3 cut(s) 823, 3019, 3067
BcnI CCSGG 1 cut(s) 2687
BcoDI GTCTC 3 cut(s) 173, 2249, 2898
BfaI CTAG 9 cut(s) 116, 353, 1571, 1709, 1845, 1865, 2163, 2219, 2291
BfmI CTRYAG 9 cut(s) 672, 1212, 1218, 1344, 1377, 1473, 1698, 1935, 2129
BfoI RGCGCY 3 cut(s) 240, 2628, 3175
BfuI GTATCC 3 cut(s) 823, 3019, 3067
BglII AGATCT 2 cut(s) 64, 1588
BlpI GCTNAGC 1 cut(s) 2235
BmcAI AGTACT 1 cut(s) 1781
Bme1390I CCNGG 2 cut(s) 787, 2687
Bme18I GGWCC 3 cut(s) 711, 792, 1888
BmeRI GACNNNNNGTC 2 cut(s) 1609, 1894
BmeT110I CYCGRG 1 cut(s) 591
BmgT120I GGNCC 5 cut(s) 395, 711, 792, 1888, 2684
BmiI GGNNCC 6 cut(s) 55, 712, 713, 793, 1504, 1716
BmrFI CCNGG 2 cut(s) 787, 2687
BmrI ACTGGG 1 cut(s) 2787
BmsI GCATC 6 cut(s) 1636, 2133, 2458, 2626, 2732, 2903
BmuI ACTGGG 1 cut(s) 2787
BpiI GAAGAC 1 cut(s) 2276
BplI GAGNNNNNCTC 4 cut(s) 2200, 2232, 2611, 2643
BpmI CTGGAG 2 cut(s) 314, 3102
Bpu10I CCTNAGC 1 cut(s) 3167
Bpu1102I GCTNAGC 1 cut(s) 2235
BpuEI CTTGAG 1 cut(s) 2638
BpuMI CCSGG 1 cut(s) 2687
BsaAI YACGTR 1 cut(s) 694
BsaBI GATNNNNATC 2 cut(s) 1587, 2085
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 4 cut(s) 786, 825, 1907, 2760
BsaWI WCCGGW 4 cut(s) 1285, 3035, 3090, 3103
BsaXI ACNNNNNCTCC 6 cut(s) 1903, 1933, 2756, 2786, 2869, 2899
Bsc4I CCNNNNNNNGG 5 cut(s) 216, 577, 2090, 2692, 2693
Bse118I RCCGGY 1 cut(s) 3090
Bse1I ACTGG 9 cut(s) 55, 331, 490, 1361, 1427, 2249, 2782, 2895, 3075
Bse21I CCTNAGG 2 cut(s) 863, 1892
Bse8I GATNNNNATC 2 cut(s) 1587, 2085
BseAI TCCGGA 1 cut(s) 1285
BseBI CCWGG 1 cut(s) 787
BseDI CCNNGG 4 cut(s) 786, 825, 1907, 2760
BseGI GGATG 9 cut(s) 262, 275, 1230, 1405, 2473, 2666, 2723, 2749, 2973
BseJI GATNNNNATC 2 cut(s) 1587, 2085
BseLI CCNNNNNNNGG 5 cut(s) 216, 577, 2090, 2692, 2693
BseMII CTCAG 4 cut(s) 2226, 2828, 2957, 3057
BseNI ACTGG 9 cut(s) 55, 331, 490, 1361, 1427, 2249, 2782, 2895, 3075
BseRI GAGGAG 8 cut(s) 21, 165, 377, 609, 639, 1802, 2477, 2931
BseYI CCCAGC 1 cut(s) 1649
Bsh1236I CGCG 3 cut(s) 688, 2002, 2733
BshFI GGCC 3 cut(s) 397, 2010, 2685
BshTI ACCGGT 1 cut(s) 3090
BsiHKAI GWGCWC 2 cut(s) 292, 2228
BsiHKCI CYCGRG 1 cut(s) 591
BsiSI CCGG 5 cut(s) 1286, 2687, 3036, 3091, 3104
BslFI GGGAC 2 cut(s) 697, 2984
BslI CCNNNNNNNGG 5 cut(s) 216, 577, 2090, 2692, 2693
BsmAI GTCTC 3 cut(s) 173, 2249, 2898
BsmFI GGGAC 2 cut(s) 697, 2984
BsnI GGCC 3 cut(s) 397, 2010, 2685
Bso31I GGTCTC 1 cut(s) 173
BsoBI CYCGRG 1 cut(s) 591
Bsp1286I GDGCHC 2 cut(s) 292, 2228
Bsp13I TCCGGA 1 cut(s) 1285
Bsp1720I GCTNAGC 1 cut(s) 2235
Bsp19I CCATGG 1 cut(s) 1907
Bsp68I TCGCGA 1 cut(s) 2733
BspACI CCGC 9 cut(s) 230, 663, 686, 734, 891, 1365, 1456, 1774, 2119
BspANI GGCC 3 cut(s) 397, 2010, 2685
BspCNI CTCAG 4 cut(s) 2227, 2829, 2958, 3056
BspEI TCCGGA 1 cut(s) 1285
BspFNI CGCG 3 cut(s) 688, 2002, 2733
BspLI GGNNCC 6 cut(s) 55, 712, 713, 793, 1504, 1716
BspMAI CTGCAG 5 cut(s) 676, 1222, 1348, 1702, 2133
BspPI GGATC 5 cut(s) 97, 1277, 2075, 2795, 3070
BspQI GCTCTTC 2 cut(s) 417, 1569
BspTNI GGTCTC 1 cut(s) 173
BsrFI RCCGGY 1 cut(s) 3090
BsrI ACTGG 9 cut(s) 55, 331, 490, 1361, 1427, 2249, 2782, 2895, 3075
BssAI RCCGGY 1 cut(s) 3090
BssECI CCNNGG 4 cut(s) 786, 825, 1907, 2760
BssNAI GTATAC 2 cut(s) 323, 1096
BssT1I CCWWGG 2 cut(s) 1907, 2760
Bst1107I GTATAC 2 cut(s) 323, 1096
Bst2UI CCWGG 1 cut(s) 787
Bst6I CTCTTC 6 cut(s) 125, 183, 417, 616, 1569, 1962
BstAPI GCANNNNNTGC 1 cut(s) 887
BstBAI YACGTR 1 cut(s) 694
BstC8I GCNNGC 7 cut(s) 235, 408, 1301, 1458, 2228, 2232, 2993
BstDEI CTNAG 8 cut(s) 564, 863, 1892, 2235, 2837, 2966, 3043, 3167
BstDSI CCRYGG 2 cut(s) 825, 1907
BstEII GGTNACC 1 cut(s) 638
BstF5I GGATG 9 cut(s) 262, 275, 1230, 1405, 2473, 2666, 2723, 2749, 2973
BstFNI CGCG 3 cut(s) 688, 2002, 2733
BstH2I RGCGCY 3 cut(s) 240, 2628, 3175
BstHHI GCGC 4 cut(s) 239, 1259, 2627, 3174
BstMAI GTCTC 3 cut(s) 173, 2249, 2898
BstMWI GCNNNNNNNGC 9 cut(s) 236, 403, 734, 887, 1373, 1576, 2125, 2236, 2992
BstNI CCWGG 1 cut(s) 787
BstNSI RCATGY 2 cut(s) 1396, 2651
BstPI GGTNACC 1 cut(s) 638
BstSCI CCNGG 2 cut(s) 785, 2685
BstSFI CTRYAG 9 cut(s) 672, 1212, 1218, 1344, 1377, 1473, 1698, 1935, 2129
BstUI CGCG 3 cut(s) 688, 2002, 2733
BstV2I GAAGAC 1 cut(s) 2276
BstX2I RGATCY 4 cut(s) 64, 1282, 1588, 2800
BstXI CCANNNNNNTGG 1 cut(s) 2417
BstYI RGATCY 4 cut(s) 64, 1282, 1588, 2800
BstZ17I GTATAC 2 cut(s) 323, 1096
Bsu36I CCTNAGG 2 cut(s) 863, 1892
BsuI GTATCC 3 cut(s) 823, 3019, 3067
BsuRI GGCC 3 cut(s) 397, 2010, 2685
BtgI CCRYGG 2 cut(s) 825, 1907
BtgZI GCGATG 3 cut(s) 1094, 1169, 2835
BtsCI GGATG 9 cut(s) 262, 275, 1230, 1405, 2473, 2666, 2723, 2749, 2973
BtsI GCAGTG 2 cut(s) 318, 903
BtsIMutI CAGTG 5 cut(s) 318, 903, 2888, 3068, 3190
BtuMI TCGCGA 1 cut(s) 2733
Cac8I GCNNGC 7 cut(s) 235, 408, 1301, 1458, 2228, 2232, 2993
CaiI CAGNNNCTG 3 cut(s) 887, 1306, 1472
CfoI GCGC 4 cut(s) 239, 1259, 2627, 3174
Cfr10I RCCGGY 1 cut(s) 3090
Cfr13I GGNCC 5 cut(s) 395, 711, 792, 1888, 2684
CseI GACGC 2 cut(s) 677, 2008
Csp6I GTAC 5 cut(s) 465, 975, 1449, 1780, 2913
CspAI ACCGGT 1 cut(s) 3090
CspCI CAANNNNNGTGG 2 cut(s) 2346, 2381
CviQI GTAC 5 cut(s) 465, 975, 1449, 1780, 2913
DdeI CTNAG 8 cut(s) 564, 863, 1892, 2235, 2837, 2966, 3043, 3167
DraI TTTAAA 2 cut(s) 1039, 2541
DrdI GACNNNNNNGTC 2 cut(s) 636, 2278
DriI GACNNNNNGTC 2 cut(s) 1609, 1894
DseDI GACNNNNNNGTC 2 cut(s) 636, 2278
EaeI YGGCCR 1 cut(s) 2008
Eam1104I CTCTTC 6 cut(s) 125, 183, 417, 616, 1569, 1962
Eam1105I GACNNNNNGTC 2 cut(s) 1609, 1894
EarI CTCTTC 6 cut(s) 125, 183, 417, 616, 1569, 1962
Ecl136II GAGCTC 2 cut(s) 290, 2226
Eco130I CCWWGG 2 cut(s) 1907, 2760
Eco24I GRGCYC 2 cut(s) 292, 2228
Eco31I GGTCTC 1 cut(s) 173
Eco47I GGWCC 3 cut(s) 711, 792, 1888
Eco53kI GAGCTC 2 cut(s) 290, 2226
Eco57I CTGAAG 3 cut(s) 146, 1449, 2916
Eco72I CACGTG 1 cut(s) 694
Eco81I CCTNAGG 2 cut(s) 863, 1892
Eco88I CYCGRG 1 cut(s) 591
Eco91I GGTNACC 1 cut(s) 638
EcoICRI GAGCTC 2 cut(s) 290, 2226
EcoO109I RGGNCCY 1 cut(s) 711
EcoO65I GGTNACC 1 cut(s) 638
EcoRII CCWGG 1 cut(s) 785
EcoT14I CCWWGG 2 cut(s) 1907, 2760
EcoT38I GRGCYC 2 cut(s) 292, 2228
ErhI CCWWGG 2 cut(s) 1907, 2760
FalI AAGNNNNNCTT 2 cut(s) 948, 980
FaqI GGGAC 2 cut(s) 697, 2984
FauI CCCGC 1 cut(s) 1449
FblI GTMKAC 4 cut(s) 47, 322, 603, 1095
FokI GGATG 9 cut(s) 269, 282, 1237, 1412, 2480, 2653, 2710, 2756, 2960
FriOI GRGCYC 2 cut(s) 292, 2228
FspBI CTAG 9 cut(s) 116, 353, 1571, 1709, 1845, 1865, 2163, 2219, 2291
GlaI GCGC 4 cut(s) 238, 1258, 2626, 3173
GsaI CCCAGC 1 cut(s) 1653
GsuI CTGGAG 2 cut(s) 314, 3102
HaeII RGCGCY 3 cut(s) 240, 2628, 3175
HaeIII GGCC 3 cut(s) 397, 2010, 2685
HapII CCGG 5 cut(s) 1286, 2687, 3036, 3091, 3104
HgaI GACGC 2 cut(s) 677, 2008
HhaI GCGC 4 cut(s) 239, 1259, 2627, 3174
Hin6I GCGC 4 cut(s) 237, 1257, 2625, 3172
HinP1I GCGC 4 cut(s) 237, 1257, 2625, 3172
HincII GTYRAC 2 cut(s) 48, 2775
HindII GTYRAC 2 cut(s) 48, 2775
HpaII CCGG 5 cut(s) 1286, 2687, 3036, 3091, 3104
Hpy99I CGWCG 2 cut(s) 442, 720
HpyCH4IV ACGT 3 cut(s) 693, 1129, 1881
HpyF10VI GCNNNNNNNGC 9 cut(s) 236, 403, 734, 887, 1373, 1576, 2125, 2236, 2992
HpyF3I CTNAG 8 cut(s) 564, 863, 1892, 2235, 2837, 2966, 3043, 3167
HpySE526I ACGT 3 cut(s) 693, 1129, 1881
HspAI GCGC 4 cut(s) 237, 1257, 2625, 3172
KflI GGGWCCC 1 cut(s) 711
Kpn2I TCCGGA 1 cut(s) 1285
LguI GCTCTTC 2 cut(s) 417, 1569
LmnI GCTCC 4 cut(s) 1508, 1714, 2205, 3163
LweI GCATC 6 cut(s) 1636, 2133, 2458, 2626, 2732, 2903
MaeI CTAG 9 cut(s) 116, 353, 1571, 1709, 1845, 1865, 2163, 2219, 2291
MaeII ACGT 3 cut(s) 693, 1129, 1881
MfeI CAATTG 1 cut(s) 729
MflI RGATCY 4 cut(s) 64, 1282, 1588, 2800
MhlI GDGCHC 2 cut(s) 292, 2228
MluI ACGCGT 1 cut(s) 2000
MlyI GAGTC 4 cut(s) 1429, 1619, 2519, 2776
MroI TCCGGA 1 cut(s) 1285
MroXI GAANNNNTTC 1 cut(s) 1197
MseI TTAA 6 cut(s) 542, 1038, 1136, 2526, 2540, 3189
MslI CAYNNNNRTG 5 cut(s) 824, 943, 2415, 2978, 3003
MspA1I CMGCKG 2 cut(s) 663, 1306
MspI CCGG 5 cut(s) 1286, 2687, 3036, 3091, 3104
MspR9I CCNGG 2 cut(s) 787, 2687
MunI CAATTG 1 cut(s) 729
MvaI CCWGG 1 cut(s) 787
MvnI CGCG 3 cut(s) 688, 2002, 2733
MwoI GCNNNNNNNGC 9 cut(s) 236, 403, 734, 887, 1373, 1576, 2125, 2236, 2992
NciI CCSGG 1 cut(s) 2687
NcoI CCATGG 1 cut(s) 1907
NlaIV GGNNCC 6 cut(s) 55, 712, 713, 793, 1504, 1716
NmuCI GTSAC 9 cut(s) 583, 638, 689, 694, 1117, 2003, 2789, 3046, 3120
NruI TCGCGA 1 cut(s) 2733
NspI RCATGY 2 cut(s) 1396, 2651
OliI CACNNNNGTG 2 cut(s) 824, 3003
PaeR7I CTCGAG 1 cut(s) 591
PciI ACATGT 1 cut(s) 1392
PciSI GCTCTTC 2 cut(s) 417, 1569
PcsI WCGNNNNNNNCGW 1 cut(s) 1746
PdmI GAANNNNTTC 1 cut(s) 1197
PflFI GACNNNGTC 1 cut(s) 636
PflMI CCANNNNNTGG 2 cut(s) 577, 2090
PinAI ACCGGT 1 cut(s) 3090
PleI GAGTC 4 cut(s) 1429, 1618, 2518, 2775
PmaCI CACGTG 1 cut(s) 694
PmlI CACGTG 1 cut(s) 694
PpsI GAGTC 4 cut(s) 1429, 1618, 2518, 2775
Ppu21I YACGTR 1 cut(s) 694
PpuMI RGGWCCY 1 cut(s) 711
PscI ACATGT 1 cut(s) 1392
Psp124BI GAGCTC 2 cut(s) 292, 2228
Psp5II RGGWCCY 1 cut(s) 711
Psp6I CCWGG 1 cut(s) 785
PspCI CACGTG 1 cut(s) 694
PspEI GGTNACC 1 cut(s) 638
PspFI CCCAGC 1 cut(s) 1649
PspGI CCWGG 1 cut(s) 785
PspN4I GGNNCC 6 cut(s) 55, 712, 713, 793, 1504, 1716
PspPI GGNCC 5 cut(s) 395, 711, 792, 1888, 2684
PspPPI RGGWCCY 1 cut(s) 711
PsrI GAACNNNNNNTAC 2 cut(s) 841, 873
PstI CTGCAG 5 cut(s) 676, 1222, 1348, 1702, 2133
PstNI CAGNNNCTG 3 cut(s) 887, 1306, 1472
PsuI RGATCY 4 cut(s) 64, 1282, 1588, 2800
PsyI GACNNNGTC 1 cut(s) 636
PvuII CAGCTG 1 cut(s) 1306
RruI TCGCGA 1 cut(s) 2733
RsaI GTAC 5 cut(s) 466, 976, 1450, 1781, 2914
RsaNI GTAC 5 cut(s) 465, 975, 1449, 1780, 2913
RseI CAYNNNNRTG 5 cut(s) 824, 943, 2415, 2978, 3003
SacI GAGCTC 2 cut(s) 292, 2228
SalI GTCGAC 1 cut(s) 46
SapI GCTCTTC 2 cut(s) 417, 1569
SaqAI TTAA 6 cut(s) 542, 1038, 1136, 2526, 2540, 3189
Sau96I GGNCC 5 cut(s) 395, 711, 792, 1888, 2684
ScaI AGTACT 1 cut(s) 1781
SchI GAGTC 4 cut(s) 1429, 1619, 2519, 2776
ScrFI CCNGG 2 cut(s) 787, 2687
SduI GDGCHC 2 cut(s) 292, 2228
SfaNI GCATC 6 cut(s) 1636, 2133, 2458, 2626, 2732, 2903
SfcI CTRYAG 9 cut(s) 672, 1212, 1218, 1344, 1377, 1473, 1698, 1935, 2129
Sfr274I CTCGAG 1 cut(s) 591
SinI GGWCC 3 cut(s) 711, 792, 1888
SlaI CTCGAG 1 cut(s) 591
SmiMI CAYNNNNRTG 5 cut(s) 824, 943, 2415, 2978, 3003
SmlI CTYRAG 3 cut(s) 15, 591, 2617
SmoI CTYRAG 3 cut(s) 15, 591, 2617
SsiI CCGC 9 cut(s) 230, 663, 686, 734, 891, 1365, 1456, 1774, 2119
SspMI CTAG 9 cut(s) 116, 353, 1571, 1709, 1845, 1865, 2163, 2219, 2291
SstI GAGCTC 2 cut(s) 292, 2228
StyD4I CCNGG 2 cut(s) 785, 2685
StyI CCWWGG 2 cut(s) 1907, 2760
TaiI ACGT 3 cut(s) 696, 1132, 1884
TaqI TCGA 8 cut(s) 47, 370, 592, 1740, 2110, 2478, 2808, 3144
TaqII GACCGA 2 cut(s) 2760, 3120
TatI WGTACW 1 cut(s) 1779
TauI GCSGC 4 cut(s) 233, 737, 893, 1367
Tru1I TTAA 6 cut(s) 542, 1038, 1136, 2526, 2540, 3189
Tru9I TTAA 6 cut(s) 542, 1038, 1136, 2526, 2540, 3189
TscAI CASTG 5 cut(s) 318, 910, 2895, 3075, 3190
TseFI GTSAC 9 cut(s) 583, 638, 689, 694, 1117, 2003, 2789, 3046, 3120
Tsp45I GTSAC 9 cut(s) 583, 638, 689, 694, 1117, 2003, 2789, 3046, 3120
TspDTI ATGAA 9 cut(s) 170, 1172, 1190, 1302, 1599, 2052, 2409, 2553, 3173
TspGWI ACGGA 3 cut(s) 455, 779, 814
TspRI CASTG 5 cut(s) 318, 910, 2895, 3075, 3190
Tth111I GACNNNGTC 1 cut(s) 636
Van91I CCANNNNNTGG 2 cut(s) 577, 2090
VpaK11BI GGWCC 3 cut(s) 711, 792, 1888
XapI RAATTY 3 cut(s) 38, 148, 2705
XbaI TCTAGA 1 cut(s) 2218
XceI RCATGY 2 cut(s) 1396, 2651
XhoI CTCGAG 1 cut(s) 591
XmiI GTMKAC 4 cut(s) 47, 322, 603, 1095
XmnI GAANNNNTTC 1 cut(s) 1197
XspI CTAG 9 cut(s) 116, 353, 1571, 1709, 1845, 1865, 2163, 2219, 2291
ZrmI AGTACT 1 cut(s) 1781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.