Rroxscaffold_6G00388860

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004406
Physical Location & Seq
Reverse (-)
2186395 .. 2196797
10403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00388860.1

Sequence Viewer

Length: 3243 bp
ATGGATGTTTCACTTCAAGGAAAAAAGCAGAATTTGCAGACACATGCTCGTAGTAAAGTTTTGTCAAAGGGAGCCCACAAAGATCTGTGGCTTGTTGTTCAAGAAGGGTCATTGGCTGATGTGGATTCAGCACTAGCCTTTGTGAAGAAGAGTGGTAGTAATATTAATTCAAGAAACATATTTGGTCTCACTCCTCTTCATATTGCAACCTGGAGAAATCACATTCCCATTGTTAGGAGGCTTCTTGCAGCTGGTGCTGACCCAGATGCTAGGGATGGGGAATCAGGATGGAGTAGCCTTCACAGAGCTCTGCATTTTGGTCATCTTGCTGTGGCAAGTATACTTCTCCAGTGTGGTGCTTCTATCACACTGGAGGACTCTAAATGCCGAACACCTGTTGATCTGATCTCTGGGCCTGTGTTGCAAGTTCTTGGTAGTGGACGGACTTCAGTAACTACAGAGGTTTTCAGTTGGGGAAGTGGCACAAACTATCAACTTGGAACTGGAAATGCACACATACAAAAGTTGCCATGCAAGGTTGATGCTCTTCATGATTCTCTAATCAAGTTCGTTTCTGCCGCTAAATTCCATAGTGTAGCAGTCACTGCTCGAGGAGAAGTCTATACTTGGGGATTTGGAAGAGGAGGACGACTTGGGCACCCTGATTTTGATATACACAGCGGTCAAGCTGCGGTTATCACTCCTCGGCAGGTAACGTCTGGTCTAGGGTCCCGTCGTGTGAAGGCAGTTGCTGCAGCTAAACATCACACTGTTATTGCAACAGATGTTGGGGAGGTGTTCACCTGGGGATCCAACCGAGAGGGTCAGCTTGGCTACACTTCTGTGGACACCCAACCTACACCTCGTAGGGTGAGTTCCCTCAGGTCGAAGGTTGTCGCTGTTGCTGCAGCAAACAAGCACACTGCTGTTGTTTCTGATACAGGTGAAGTTTTCACATGGGGTGGCAACAGAGAGGGCCAGCTTGGCTATGGTACATCCAACTCTGCATCAAACTACACTCCTAGAGTGGTTGAATATTTGAAAGGCAAGGTTTTTACTGGGGTTGCAGCAGCAAAATATCACACACTTGTCTTGGGAGTTGATGGAGAGGTTTACACTTGGGGTCATCGACTTGTTACGCCAAAACGGGTTGTTGTCTCTAGAAATTTGAAAAAGGGTGGAAATTCCACTTTGAAGTTTCATCGCAAGGAACGGCTCCATGTGGTTTCAATAGCTGCAGGGGCGGTACATAGCATGGCTCTCACCGATGATGGTGCTTTGTTTTATTGGGTTTCTTCGGATCCTGATCTTAGATGCCAACAGGTTTACTTCATGACACCAGTAAATTTGGTGAACATATCTGCAGGGAAGTACTGGACTGCTGCTGTTACAGCTACAGGTGATATTTACATGTGGGATGGGAAAAATGGTAAGGATAAGCCACCAATTGCAACTCGGTTACATGGTACAAAGCGGGCTACTTCCGTTTCAGTTGGTGAAACGCATTTGTTAATTATTGGTTCTCTTTATCATCCTGCCTACACTTCCAATGTGGTGGAGGTTCCACATAAGAAGTCAATTATCAGAGATGAACTAGAAGAAATTGATGAAGATCCTATGTTTGATGACATGTGTTCAGAGAATCCTTTACCTATTGTACAAGATGATGATTCCAGCAAGAAAGCCGTACCAAGCTTAAAAAGTATTTGTGAAAAAGTGGCTGCAGAGAAATTAGTGGAGCCACGAAATGCTATTCAACTGCTTGAAATTGCAGATTCACTCATGGCAGATGATCTAAGGAAGTATTGTGAGGAAATTGCTATACGGAACCTTGACTATATTTTCACCGTATCATCACAAGCTATTGCTGGAGCTTCACCAGATATTCTAGCTAACCTCGAAAAATCATTGGATTTGAAGTCATCTGAACCATGGAGCTACCGTCGTCTCCCAACTCCAACAGCTACTTTCCCTGCTGTTATTTATAGTGAGGAGGAAGATAGTGAGAGTGAGGTTCAAAGAACACGTGACAGCCATACCAAGCAATCTACCTCCAAAAATGGAATACACCACAGACCAGATTCCTTTCTACAACCCAAAGATGATCCAAATCAAGGTATTGGCAAGGAAGTTCGAGCCTTGCGGAAAAAGCTGCAGCAGATTGAGATGCTTGAAGAAAAACAATCTAATGGGTATCTTCTTGATGACCAGCAAATTAAAAAGCTTCAAACAAGGTCAGCTCTAGAGAGTTCACTTGCTGAGCTCGGGGTTCCAGTTGAGACAACAGAGTTGAAGGCATCATCTTCAGTGCTGCCAGATGGAAAAGGAAACAAAAAGGTTGAGCTTTCAAGAAAACAGAGGAGAAAGAACAAACAGACCGCAATGCAAGTGCCTCGGTTTCCTGGAAGTGAAGCAGAACCAAACTCAGTAAAAGGTTTCTTGAATAGTGGAATCTCTCAAAACTCAGAGAATAAGGAGGAAGCTTCCATGGTTGGAGAGATTATGACCAGCCAAACGACTACAGAGTCAGCTTTATGTGTCCCGAAAGAGAAGTTAGACTCAACAAAGAACCACTTGCCATCACCAACAGTTTCCAAGAAGAAAAGTAAAAAGGGTGGGCTTTCAATGTTCTTGAGTGGTGCACTTGATGACTCCCCTAAATATATTGCTCCTCCACCAACCCAAAAAAGTGAGGGTCCTGCATGGGGTGGGGCTAAAATTTCCAAAGGATTTGCGTCTCTTCGTGACATTCAAGATGAGCAAATTAAAATTAAGGAGCATCAGCCAACTAGGAACAAAGATCCCTTGGAAGAACCTGTCATCGCTAAAGGTGATGGGAAGATCCTATTGAGTTCGTTTTTGCCTTCTAAGCCAATCCCAGTAGGCTCAGTTCCAGTTGTCTCAAACCCTGCTTCACTGGCAAATGAAGGAGATAGAAGTACACCTCCTTGGACTGCTTCAGGAACTCCTCCGCTTCTTTCACGTCCATCTCTTAGGGACATCCAAATGCAGCAGAAGGGAAAACAACAGCATAATCTGTCTCACAGTCCAAAGACAAAAACAGCTGGGTTCTCAGTCACTACTGGTCAAGGTTCACCATTGGACTCTTCTGGATTGAACCGCTGGTTCAAACCGGAGGTGGATGCACCCTCATCTATTCGTTCAATCCAGATTGAGGAAAAGGCCATGAAGGATCTTAGGCGCTTCTACAAGAGTGTTAAGGTTGTCAAGAACCCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_4 Ank_5 RCC1

Protein Analysis

1080

Amino Acids

117.64

Weight (kDa)

9.27

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 32 - 126 2.9e-12 Ankyrin repeats (3 copies)
Ank_5 PF13857 53 - 103 1.3e-07 Ankyrin repeats (many copies)
Ank PF00023 62 - 92 1.2e-07 Ankyrin repeat
Ank_4 PF13637 73 - 116 1.2e-07 Ankyrin repeats (many copies)
WD40_RLD PF25390 154 - 375 1.2e-49 RCC1-like domain
RCC1 PF00415 154 - 201 3.2e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 205 - 259 8e-09 Regulator of chromosome condensation (RCC1) repeat
WD40_RLD PF25390 235 - 430 2.8e-40 RCC1-like domain
RCC1 PF00415 264 - 311 3.9e-13 Regulator of chromosome condensation (RCC1) repeat
WD40_RLD PF25390 296 - 504 2.6e-31 RCC1-like domain
RCC1_2 PF13540 298 - 327 4.3e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 315 - 365 1.7e-11 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2524
Acc36I ACCTGC 1 cut(s) 700
AccB1I GGYRCC 1 cut(s) 657
AccI GTMKAC 1 cut(s) 340
AciI CCGC 9 cut(s) 579, 681, 692, 1244, 1474, 2143, 2379, 2972, 3121
AclWI GGATC 9 cut(s) 804, 817, 1295, 1308, 1607, 2099, 2795, 2836, 3201
AcsI RAATTY 6 cut(s) 31, 584, 1165, 1183, 1345, 2718
AcuI CTGAAG 3 cut(s) 432, 2289, 2943
AcvI CACGTG 1 cut(s) 2027
AfaI GTAC 7 cut(s) 994, 1248, 1373, 1468, 1659, 1689, 2941
AfiI CCNNNNNNNGG 4 cut(s) 234, 2114, 2705, 3175
AflIII ACRYGT 3 cut(s) 1410, 1629, 2024
AjiI CACGTC 1 cut(s) 2984
AjnI CCWGG 3 cut(s) 209, 803, 2401
AleI CACNNNNGTG 1 cut(s) 842
Alw21I GWGCWC 3 cut(s) 310, 2265, 2644
Alw26I GTCTC 7 cut(s) 191, 1162, 1952, 2273, 2742, 2905, 3045
Alw44I GTGCAC 1 cut(s) 2640
AlwI GGATC 9 cut(s) 804, 817, 1295, 1308, 1607, 2099, 2795, 2836, 3201
AlwNI CAGNNNCTG 2 cut(s) 605, 752
Ama87I CYCGRG 2 cut(s) 609, 2264
AoxI GGCC 3 cut(s) 413, 976, 3183
ApaLI GTGCAC 1 cut(s) 2640
ApoI RAATTY 6 cut(s) 31, 584, 1165, 1183, 1345, 2718
AseI ATTAAT 1 cut(s) 165
AspLEI GCGC 1 cut(s) 3204
AspS9I GGNCC 4 cut(s) 413, 729, 976, 2696
AvaI CYCGRG 2 cut(s) 609, 2264
AvaII GGWCC 2 cut(s) 729, 2696
AxyI CCTNAGG 1 cut(s) 881
BaeGI GKGCMC 2 cut(s) 660, 2644
BamHI GGATCC 2 cut(s) 809, 1300
BanI GGYRCC 1 cut(s) 657
BanII GRGCYC 3 cut(s) 76, 310, 2265
BbrPI CACGTG 1 cut(s) 2027
Bbv12I GWGCWC 3 cut(s) 310, 2265, 2644
BccI CCATC 9 cut(s) 269, 282, 1097, 1265, 1412, 2312, 2587, 2828, 2995
BceAI ACGGC 2 cut(s) 1229, 1670
BcgI CGANNNNNNTGC 4 cut(s) 1256, 1290, 2375, 2409
BciT130I CCWGG 3 cut(s) 211, 805, 2403
BcoDI GTCTC 7 cut(s) 191, 1162, 1952, 2273, 2742, 2905, 3045
BfaI CTAG 9 cut(s) 134, 270, 725, 1023, 1161, 1595, 1889, 2243, 2790
BfmI CTRYAG 9 cut(s) 456, 753, 906, 1236, 1362, 1395, 1722, 2153, 2520
BfoI RGCGCY 1 cut(s) 3205
BfuAI ACCTGC 1 cut(s) 700
BglI GCCNNNNNGGC 1 cut(s) 984
BglII AGATCT 1 cut(s) 82
BlpI GCTNAGC 1 cut(s) 2259
BmcAI AGTACT 1 cut(s) 1373
Bme1390I CCNGG 3 cut(s) 211, 805, 2403
Bme18I GGWCC 2 cut(s) 729, 2696
BmeT110I CYCGRG 2 cut(s) 609, 2264
BmgBI CACGTC 1 cut(s) 2984
BmgT120I GGNCC 4 cut(s) 413, 729, 976, 2696
BmrFI CCNGG 3 cut(s) 211, 805, 2403
BmrI ACTGGG 2 cut(s) 1068, 2873
BmsI GCATC 8 cut(s) 256, 532, 1016, 1304, 2157, 2306, 2788, 3133
BmuI ACTGGG 2 cut(s) 1068, 2873
BpmI CTGGAG 4 cut(s) 232, 332, 392, 1890
Bpu1102I GCTNAGC 1 cut(s) 2259
BpuEI CTTGAG 1 cut(s) 2653
BsaAI YACGTR 1 cut(s) 2027
BsaBI GATNNNNATC 3 cut(s) 1305, 1611, 2109
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 7 cut(s) 704, 804, 1931, 2393, 2487, 2805, 2948
BsaWI WCCGGW 1 cut(s) 3133
BsaXI ACNNNNNCTCC 2 cut(s) 1927, 1957
Bsc4I CCNNNNNNNGG 4 cut(s) 234, 2114, 2705, 3175
Bse21I CCTNAGG 1 cut(s) 881
Bse3DI GCAATG 1 cut(s) 2388
Bse8I GATNNNNATC 3 cut(s) 1305, 1611, 2109
BseBI CCWGG 3 cut(s) 211, 805, 2403
BseDI CCNNGG 7 cut(s) 704, 804, 1931, 2393, 2487, 2805, 2948
BseGI GGATG 8 cut(s) 10, 280, 293, 995, 1423, 1531, 3000, 3148
BseJI GATNNNNATC 3 cut(s) 1305, 1611, 2109
BseLI CCNNNNNNNGG 4 cut(s) 234, 2114, 2705, 3175
BseMI GCAATG 1 cut(s) 2388
BseMII CTCAG 6 cut(s) 895, 2250, 2439, 2478, 2901, 3087
BseRI GAGGAG 8 cut(s) 183, 627, 657, 693, 2006, 2374, 2661, 2958
BseSI GKGCMC 2 cut(s) 660, 2644
BseYI CCCAGC 1 cut(s) 3065
BshFI GGCC 3 cut(s) 415, 978, 3185
BshNI GGYRCC 1 cut(s) 657
BsiHKAI GWGCWC 3 cut(s) 310, 2265, 2644
BsiHKCI CYCGRG 2 cut(s) 609, 2264
BsiSI CCGG 1 cut(s) 3134
BslFI GGGAC 3 cut(s) 715, 2525, 3011
BslI CCNNNNNNNGG 4 cut(s) 234, 2114, 2705, 3175
BsmAI GTCTC 7 cut(s) 191, 1162, 1952, 2273, 2742, 2905, 3045
BsmBI CGTCTC 2 cut(s) 1952, 2742
BsmFI GGGAC 3 cut(s) 715, 2525, 3011
BsnI GGCC 3 cut(s) 415, 978, 3185
Bso31I GGTCTC 1 cut(s) 191
BsoBI CYCGRG 2 cut(s) 609, 2264
Bsp1286I GDGCHC 5 cut(s) 76, 310, 660, 2265, 2644
Bsp1407I TGTACA 1 cut(s) 1657
Bsp1720I GCTNAGC 1 cut(s) 2259
Bsp19I CCATGG 2 cut(s) 1931, 2487
BspACI CCGC 9 cut(s) 579, 681, 692, 1244, 1474, 2143, 2379, 2972, 3121
BspANI GGCC 3 cut(s) 415, 978, 3185
BspCNI CTCAG 6 cut(s) 894, 2251, 2438, 2477, 2900, 3086
BspHI TCATGA 2 cut(s) 550, 1332
BspMAI CTGCAG 6 cut(s) 757, 910, 1240, 1366, 1726, 2157
BspMI ACCTGC 1 cut(s) 700
BspPI GGATC 9 cut(s) 804, 817, 1295, 1308, 1607, 2099, 2795, 2836, 3201
BspQI GCTCTTC 1 cut(s) 552
BspT107I GGYRCC 1 cut(s) 657
BspTNI GGTCTC 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 2388
BsrGI TGTACA 1 cut(s) 1657
BssECI CCNNGG 7 cut(s) 704, 804, 1931, 2393, 2487, 2805, 2948
BssNAI GTATAC 1 cut(s) 341
BssT1I CCWWGG 4 cut(s) 1931, 2487, 2805, 2948
Bst1107I GTATAC 1 cut(s) 341
Bst2UI CCWGG 3 cut(s) 211, 805, 2403
Bst4CI ACNGT 5 cut(s) 772, 1849, 1943, 2590, 3047
Bst6I CTCTTC 6 cut(s) 143, 201, 552, 634, 2745, 3112
BstAPI GCANNNNNTGC 4 cut(s) 34, 254, 605, 752
BstAUI TGTACA 1 cut(s) 1657
BstBAI YACGTR 1 cut(s) 2027
BstC8I GCNNGC 2 cut(s) 980, 1476
BstDSI CCRYGG 2 cut(s) 1931, 2487
BstF5I GGATG 8 cut(s) 10, 280, 293, 995, 1423, 1531, 3000, 3148
BstH2I RGCGCY 1 cut(s) 3205
BstHHI GCGC 1 cut(s) 3204
BstMAI GTCTC 7 cut(s) 191, 1162, 1952, 2273, 2742, 2905, 3045
BstNI CCWGG 3 cut(s) 211, 805, 2403
BstNSI RCATGY 3 cut(s) 47, 1414, 1633
BstSCI CCNGG 3 cut(s) 209, 803, 2401
BstSFI CTRYAG 9 cut(s) 456, 753, 906, 1236, 1362, 1395, 1722, 2153, 2520
BstSLI GKGCMC 2 cut(s) 660, 2644
BstX2I RGATCY 7 cut(s) 82, 809, 1300, 1612, 2800, 2841, 3193
BstXI CCANNNNNNTGG 1 cut(s) 1555
BstYI RGATCY 7 cut(s) 82, 809, 1300, 1612, 2800, 2841, 3193
BstZ17I GTATAC 1 cut(s) 341
Bsu36I CCTNAGG 1 cut(s) 881
BsuRI GGCC 3 cut(s) 415, 978, 3185
BtgI CCRYGG 2 cut(s) 1931, 2487
BtgZI GCGATG 2 cut(s) 1187, 2806
BtrI CACGTC 1 cut(s) 2984
BtsCI GGATG 8 cut(s) 10, 280, 293, 995, 1423, 1531, 3000, 3148
BtsI GCAGTG 2 cut(s) 603, 921
BtsIMutI CAGTG 7 cut(s) 356, 368, 603, 768, 921, 2313, 2915
BveI ACCTGC 1 cut(s) 700
Cac8I GCNNGC 2 cut(s) 980, 1476
CaiI CAGNNNCTG 2 cut(s) 605, 752
CciI TCATGA 2 cut(s) 550, 1332
CfoI GCGC 1 cut(s) 3204
Cfr13I GGNCC 4 cut(s) 413, 729, 976, 2696
CseI GACGC 1 cut(s) 2724
Csp6I GTAC 7 cut(s) 993, 1247, 1372, 1467, 1658, 1688, 2940
CviQI GTAC 7 cut(s) 993, 1247, 1372, 1467, 1658, 1688, 2940
DrdI GACNNNNNNGTC 1 cut(s) 2524
DseDI GACNNNNNNGTC 1 cut(s) 2524
Eam1104I CTCTTC 6 cut(s) 143, 201, 552, 634, 2745, 3112
EarI CTCTTC 6 cut(s) 143, 201, 552, 634, 2745, 3112
Ecl136II GAGCTC 2 cut(s) 308, 2263
Eco130I CCWWGG 4 cut(s) 1931, 2487, 2805, 2948
Eco24I GRGCYC 3 cut(s) 76, 310, 2265
Eco31I GGTCTC 1 cut(s) 191
Eco47I GGWCC 2 cut(s) 729, 2696
Eco53kI GAGCTC 2 cut(s) 308, 2263
Eco57I CTGAAG 3 cut(s) 432, 2289, 2943
Eco72I CACGTG 1 cut(s) 2027
Eco81I CCTNAGG 1 cut(s) 881
Eco88I CYCGRG 2 cut(s) 609, 2264
EcoICRI GAGCTC 2 cut(s) 308, 2263
EcoO109I RGGNCCY 2 cut(s) 729, 2696
EcoRII CCWGG 3 cut(s) 209, 803, 2401
EcoT14I CCWWGG 4 cut(s) 1931, 2487, 2805, 2948
EcoT38I GRGCYC 3 cut(s) 76, 310, 2265
ErhI CCWWGG 4 cut(s) 1931, 2487, 2805, 2948
Esp3I CGTCTC 2 cut(s) 1952, 2742
FalI AAGNNNNNCTT 2 cut(s) 2558, 2590
FaqI GGGAC 3 cut(s) 715, 2525, 3011
FauI CCCGC 1 cut(s) 1467
FblI GTMKAC 1 cut(s) 340
FokI GGATG 8 cut(s) 17, 287, 300, 982, 1430, 1518, 2987, 3155
FriOI GRGCYC 3 cut(s) 76, 310, 2265
FspBI CTAG 9 cut(s) 134, 270, 725, 1023, 1161, 1595, 1889, 2243, 2790
GlaI GCGC 1 cut(s) 3203
GsaI CCCAGC 1 cut(s) 3069
GsuI CTGGAG 4 cut(s) 232, 332, 392, 1890
HaeII RGCGCY 1 cut(s) 3205
HaeIII GGCC 3 cut(s) 415, 978, 3185
HapII CCGG 1 cut(s) 3134
HgaI GACGC 1 cut(s) 2724
HhaI GCGC 1 cut(s) 3204
Hin6I GCGC 1 cut(s) 3202
HinP1I GCGC 1 cut(s) 3202
HindIII AAGCTT 3 cut(s) 1693, 2222, 2481
HpaII CCGG 1 cut(s) 3134
Hpy188I TCNGA 7 cut(s) 405, 937, 1300, 1586, 1639, 1927, 2467
Hpy99I CGWCG 2 cut(s) 738, 1947
HpyAV CCTTC 9 cut(s) 98, 308, 736, 883, 2287, 2874, 2921, 3010, 3184
HpyCH4III ACNGT 5 cut(s) 772, 1849, 1943, 2590, 3047
HpyCH4IV ACGT 3 cut(s) 716, 2026, 2983
HpySE526I ACGT 3 cut(s) 716, 2026, 2983
HspAI GCGC 1 cut(s) 3202
KflI GGGWCCC 1 cut(s) 729
LguI GCTCTTC 1 cut(s) 552
LmnI GCTCC 7 cut(s) 71, 1221, 1738, 1871, 1935, 2674, 2776
LweI GCATC 8 cut(s) 256, 532, 1016, 1304, 2157, 2306, 2788, 3133
MaeI CTAG 9 cut(s) 134, 270, 725, 1023, 1161, 1595, 1889, 2243, 2790
MaeII ACGT 3 cut(s) 716, 2026, 2983
MaeIII GTNAC 9 cut(s) 451, 601, 712, 1135, 1387, 1458, 2027, 2744, 3076
MfeI CAATTG 1 cut(s) 1446
MflI RGATCY 7 cut(s) 82, 809, 1300, 1612, 2800, 2841, 3193
MhlI GDGCHC 5 cut(s) 76, 310, 660, 2265, 2644
MlyI GAGTC 5 cut(s) 371, 2534, 2552, 2645, 3098
MmeI TCCRAC 4 cut(s) 837, 1023, 1982, 2473
MseI TTAA 8 cut(s) 165, 1511, 1697, 2217, 2766, 2772, 3219, 3241
MslI CAYNNNNRTG 2 cut(s) 842, 3005
MspA1I CMGCKG 4 cut(s) 251, 681, 3065, 3123
MspI CCGG 1 cut(s) 3134
MspR9I CCNGG 3 cut(s) 211, 805, 2403
MunI CAATTG 1 cut(s) 1446
MvaI CCWGG 3 cut(s) 211, 805, 2403
NcoI CCATGG 2 cut(s) 1931, 2487
NmeAIII GCCGAG 1 cut(s) 685
NmuCI GTSAC 4 cut(s) 601, 2027, 2744, 3076
NspI RCATGY 3 cut(s) 47, 1414, 1633
OliI CACNNNNGTG 1 cut(s) 842
PaeR7I CTCGAG 1 cut(s) 609
PagI TCATGA 2 cut(s) 550, 1332
PciI ACATGT 2 cut(s) 1410, 1629
PciSI GCTCTTC 1 cut(s) 552
PfeI GAWTC 8 cut(s) 125, 281, 554, 1642, 1670, 1775, 2081, 2451
PfoI TCCNGGA 1 cut(s) 2401
PleI GAGTC 5 cut(s) 371, 2533, 2552, 2645, 3098
PmaCI CACGTG 1 cut(s) 2027
PmlI CACGTG 1 cut(s) 2027
PpsI GAGTC 5 cut(s) 371, 2533, 2552, 2645, 3098
Ppu21I YACGTR 1 cut(s) 2027
PpuMI RGGWCCY 2 cut(s) 729, 2696
PscI ACATGT 2 cut(s) 1410, 1629
PshBI ATTAAT 1 cut(s) 165
Psp124BI GAGCTC 2 cut(s) 310, 2265
Psp5II RGGWCCY 2 cut(s) 729, 2696
Psp6I CCWGG 3 cut(s) 209, 803, 2401
PspCI CACGTG 1 cut(s) 2027
PspFI CCCAGC 1 cut(s) 3065
PspGI CCWGG 3 cut(s) 209, 803, 2401
PspPI GGNCC 4 cut(s) 413, 729, 976, 2696
PspPPI RGGWCCY 2 cut(s) 729, 2696
PspXI VCTCGAGB 1 cut(s) 609
PsrI GAACNNNNNNTAC 4 cut(s) 859, 891, 2874, 2906
PstI CTGCAG 6 cut(s) 757, 910, 1240, 1366, 1726, 2157
PstNI CAGNNNCTG 2 cut(s) 605, 752
PsuI RGATCY 7 cut(s) 82, 809, 1300, 1612, 2800, 2841, 3193
PvuII CAGCTG 2 cut(s) 251, 3065
RsaI GTAC 7 cut(s) 994, 1248, 1373, 1468, 1659, 1689, 2941
RsaNI GTAC 7 cut(s) 993, 1247, 1372, 1467, 1658, 1688, 2940
RseI CAYNNNNRTG 2 cut(s) 842, 3005
SacI GAGCTC 2 cut(s) 310, 2265
SapI GCTCTTC 1 cut(s) 552
SaqAI TTAA 8 cut(s) 165, 1511, 1697, 2217, 2766, 2772, 3219, 3241
Sau96I GGNCC 4 cut(s) 413, 729, 976, 2696
ScaI AGTACT 1 cut(s) 1373
SchI GAGTC 5 cut(s) 371, 2534, 2552, 2645, 3098
ScrFI CCNGG 3 cut(s) 211, 805, 2403
SduI GDGCHC 5 cut(s) 76, 310, 660, 2265, 2644
SfaNI GCATC 8 cut(s) 256, 532, 1016, 1304, 2157, 2306, 2788, 3133
SfcI CTRYAG 9 cut(s) 456, 753, 906, 1236, 1362, 1395, 1722, 2153, 2520
Sfr274I CTCGAG 1 cut(s) 609
SinI GGWCC 2 cut(s) 729, 2696
SlaI CTCGAG 1 cut(s) 609
SmiMI CAYNNNNRTG 2 cut(s) 842, 3005
SmlI CTYRAG 2 cut(s) 609, 2632
SmoI CTYRAG 2 cut(s) 609, 2632
SsiI CCGC 9 cut(s) 579, 681, 692, 1244, 1474, 2143, 2379, 2972, 3121
SspI AATATT 2 cut(s) 163, 1037
SspMI CTAG 9 cut(s) 134, 270, 725, 1023, 1161, 1595, 1889, 2243, 2790
SstI GAGCTC 2 cut(s) 310, 2265
StyD4I CCNGG 3 cut(s) 209, 803, 2401
StyI CCWWGG 4 cut(s) 1931, 2487, 2805, 2948
TaaI ACNGT 5 cut(s) 772, 1849, 1943, 2590, 3047
TaiI ACGT 3 cut(s) 719, 2029, 2986
TaqI TCGA 5 cut(s) 610, 887, 1129, 1899, 2134
TatI WGTACW 3 cut(s) 1371, 1657, 2939
TauI GCSGC 1 cut(s) 581
TfiI GAWTC 8 cut(s) 125, 281, 554, 1642, 1670, 1775, 2081, 2451
Tru1I TTAA 8 cut(s) 165, 1511, 1697, 2217, 2766, 2772, 3219, 3241
Tru9I TTAA 8 cut(s) 165, 1511, 1697, 2217, 2766, 2772, 3219, 3241
TscAI CASTG 7 cut(s) 356, 375, 610, 775, 928, 2313, 2922
TseFI GTSAC 4 cut(s) 601, 2027, 2744, 3076
Tsp45I GTSAC 4 cut(s) 601, 2027, 2744, 3076
TspDTI ATGAA 8 cut(s) 188, 539, 1190, 1321, 1605, 1623, 2940, 3203
TspGWI ACGGA 3 cut(s) 457, 1474, 1840
TspRI CASTG 7 cut(s) 356, 375, 610, 775, 928, 2313, 2922
VneI GTGCAC 1 cut(s) 2640
VpaK11BI GGWCC 2 cut(s) 729, 2696
VspI ATTAAT 1 cut(s) 165
XapI RAATTY 6 cut(s) 31, 584, 1165, 1183, 1345, 2718
XbaI TCTAGA 2 cut(s) 1160, 2242
XceI RCATGY 3 cut(s) 47, 1414, 1633
XcmI CCANNNNNNNNNTGG 1 cut(s) 986
XhoI CTCGAG 1 cut(s) 609
XmiI GTMKAC 1 cut(s) 340
XspI CTAG 9 cut(s) 134, 270, 725, 1023, 1161, 1595, 1889, 2243, 2790
ZrmI AGTACT 1 cut(s) 1373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.