RLG00000022512

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004406
Physical Location & Seq
Reverse (-)
2355539 .. 2362981
7443 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022512

Sequence Viewer

Length: 3240 bp
ATGGATGTTTCACTTCAAGGAAAAAAACAGAATTTGCAGACACATGCTCGTAGTAAAGTTTTGTCAAGGGGAGCCCACAAAGATCTGTGGCTTGTTGTACAAGAAGGATCATTGGCTGATGTGGATTCAGCACTAGCCTTTGTGAAGAAGAGTGGTAGTAATATTAATTCAAGAAACATATTTGGTCTCACTCCTCTTCATATTGCAACCTGGAGAAATCACATTCCCATTGTTAGGAGGCTTCTTGCAGCTGGTGCTGACCCAGATGCTAGGGATGGGGAATCAGGATGGAGTAGCCTTCACAGAGCTCTGCATTTTGGTCATCTTGCTGTGGCAAGTATACTTCTCCAGTGTGGTGCTTCTATCACACTGGAGGACTCTAAATACCGAACACCTGTTGATCTGATATCTGGGCCTGTGTTGCAAGTTCTTGGTAGTGGACGGACTTCAGTAACTACAGAGGTTTTCAGTTGGGGAAGTGGCACAAACTATCAACTTGGAACTGGAAATGCACACATACAAAAGTTGCCATGCAAGGTTGATGCTCTTCATGATTCTCTAATCAGGTTCGTTTCTGCTGCTAAATTCCATAGTGTAGCAGTCACTGCTCGAGGAGAAGTCTATACTTGGGGATTTGGAAGAGGAGGACGACTTGGGCACCCTGATTTTGATATACACAGCGGTCAAGCTGCGGTTATCACTCCTCGGCAGGTAACGTCTGGTCTAGGGTCCCGTCGTGTGAAGGCAGTTGCTGCAGCTAAACATCACACTGTTATTGCAACAGATGTTGGGGAGGTGTTCACCTGGGGATCCAACAGAGAGGGTCAGCTTGGCTACACTTCTGTGGACACCCAACCTACGCCTCGTAGGGTGAGTTCCCAAAGGTCGAAGGTTGTCGCTGTTGCTGCAGCAAACAAGCACACTGCTGTTGTTTCTGATACAGGTGAAGTTTTCACATGGGGTGGCAACAGAGAGGGCCAGCTTGGCTATGGTACATCCAACTCTGCATCAAACTACACTCCTAGAGTGGTTGAATATTTGAAAGGCAAGGTTTTTACTGGGGTTGCAGCAGCAAAATATCACACACTTGTCTTGGGAGTTGATGGAGAGGTTTACACTTGGGGTCATCGACTTGTTACGCCAAAACGGGTTGTTGTCTCTAGAAATTTGAAAAAGGGTGGAAATTCCACTTTGAAGTTTCATCGCAAGGAACGGCTCCATGTGGTTTCAATAGCTGCAGGGGCGGTACATAGCATGGCTCTCACCGATGATGGTGCTTTGTTTTATTGGGTTTCTTCGGATCCTGATCTTAGATGCCAACAGCTTTACTCGCTGTGCGGAAGAAATTTGGTGAACATATCTGCAGGGAAGTACTGGACTGCTGCTGTTACAGCTACAGGTGATGTTTACATGTGGGATGGGAAAAATGGTAAGGATAAGCCACCAATTGCAACTCGGTTACATGGTACAAAGCGGGCTACTTCTGTTTCAGTTGGTGAAACGCATTTGTTAATTATTGGTTCTCTTTATCATCCTGCCTACACTTCCAATGTGGTGGAGGTTCCACAAAAGAAGTCAATTATCAGAGATGAACTAGAAGAAATTGATGAAGATCTTATGTTTGATGACATGTGCTCAGAGAATCCTTTACCTATTGTACAAGATGATGATTCCAGCAAGAAATCCGTACCAAGCTTAAAAAGTATTTGTGAAAAAGTGGCTGCAGAGAAATTAGTGGAGCCACGAAATGCTATTCAACTGCTTGAAATTGCAGATTCACTCATGGCAGATGATCTAAGGAAGTATTGTGAGGAAATTGCTATACGGAACCTTGACTATATTTTCACCGTATCATCACAAGCTATTGCTGGAGCTTCACCAGATATTCTAGCTAACCTTGAAAAATCATTGGATTTGAAGTCATCTGAACCATGGAGCTACCGTCGTCTCCCAACTCCAACAGCTACTTTCCCTGCTGTTATTTATAGTGAGGAGGAAGATAGTGAGAGTGATGTTCAAAGAACACGTGACAGCCATACCAAGCAATCTACCTCCAAAAATGGAATACACCACAGACCAGATTCCTTTCTGCAACCCAAAGATGATCCAAATCAAGGTATTGGCAAGGAAGTTCGAGCCTTGCGGAAAAAGCTGCAGCAGATTGAGATGCTTGAAGAAAAACAATCAAATGGATATCTTCTTGATGACCAGCAAATTAAAAAGCTTCAAACAAGGTCAGCTCTAGAGAGTTCACTTGCTGAGCTTGGGGTTCCAGTTGAGACAACAGAGTTGAAGGCATCACCTTCTGTGCTGCCAGATGGAAAAGGAAACAAAAAGGTTGAGCTTTCAAGAAAACAGAGGAGAAAGAACAAACAGACCGCAATGCAAGTGCCTCGGTTTCCTGGAAGTGAAGCAGAACCAAACTCGGTAAAAGGTTTCTTGAATAGTGGAATCTCTCAAAACTCAGAGAAAAAGGAGGCTTCCATGGTTGGAGAGATTATGACCAACCAAACTACTACAGAGTCAGCTTTATGTGTCCCGAAAGAGAAGTTAGACTCAACAAAGAACCACTTGCCATCACCAACAGTTTCCAAGAAGAAAAGTAAAAAGGGCGGGCTTTCAATGTTCTTGAGTGGTGCACTTGATGACTCCCCTAAAGATATTGCTCCTCCACCAACCCAAAAAAGTGAGGGTCCTGCATGGGGTGGGGCTAAAATTTCCAAAGCATTTGCGTCTCTTCGTGACATTCAAGATGAGCAAATTAAAATTAAGGAGCATCAGCCAACTAGGAACAAAGATCCCTTGGAAGAACCTGTCATCGCTAAAGGTGATGGGAAGATCCTATTGAGTTCGTTTTTGCCTTCTAAGCCAATCCCAGTAGGCTCAGTACCAGTTGTCTCAAACCCTGCTTCACTGGCAAATGAAGGAGATAGAAGTACACCTCCTTGGACTGCTTCAGGAACTCCTCCGCTTCTTTCACGTCCATCTCTTAGGGACATCCAAATGCAGCAGAAGGGAAAACAACAGCATAATCTGTCTCACAGTCCAAAGACAAAAACAGCTGGGTTCTCAGTCACTACTGGTCAAGGTTCGCCATTGGACTCTTCTGGATTGAACCGCTGGTTCAAACCGGAGGTGGATGCACCCTCATCTATTCGTTCAATCCAGATCGAGGAAAAGGCCATGAAGGATCTTAGGCGCTTCTACAAGAGTGTTAAGGTTGTCAAGAACCCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_4 Ank_5 RCC1

Protein Analysis

1080

Amino Acids

117.61

Weight (kDa)

9.33

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 32 - 126 2.9e-12 Ankyrin repeats (3 copies)
Ank_5 PF13857 53 - 103 1.3e-07 Ankyrin repeats (many copies)
Ank PF00023 62 - 92 1.2e-07 Ankyrin repeat
Ank_4 PF13637 73 - 116 1.2e-07 Ankyrin repeats (many copies)
WD40_RLD PF25390 154 - 375 1.2e-49 RCC1-like domain
RCC1 PF00415 154 - 201 4.4e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 205 - 259 8e-09 Regulator of chromosome condensation (RCC1) repeat
WD40_RLD PF25390 235 - 430 1.3e-39 RCC1-like domain
RCC1 PF00415 264 - 311 3.1e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 298 - 327 4.3e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 315 - 365 1.7e-11 Regulator of chromosome condensation (RCC1) repeat
WD40_RLD PF25390 404 - 504 9.3e-09 RCC1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 700
AccB1I GGYRCC 1 cut(s) 657
AccI GTMKAC 1 cut(s) 340
AclWI GGATC 9 cut(s) 115, 804, 817, 1295, 1308, 2099, 2792, 2833, 3198
AcsI RAATTY 6 cut(s) 31, 584, 1165, 1183, 1345, 2715
AcuI CTGAAG 2 cut(s) 432, 2940
AcvI CACGTG 1 cut(s) 2027
AfaI GTAC 9 cut(s) 99, 994, 1248, 1373, 1468, 1659, 1689, 2889, 2938
AfiI CCNNNNNNNGG 4 cut(s) 234, 2114, 2702, 3172
AflIII ACRYGT 3 cut(s) 1410, 1629, 2024
AjiI CACGTC 1 cut(s) 2981
AjnI CCWGG 3 cut(s) 209, 803, 2401
AleI CACNNNNGTG 1 cut(s) 842
Alw21I GWGCWC 3 cut(s) 310, 1637, 2641
Alw26I GTCTC 7 cut(s) 191, 1162, 1952, 2273, 2739, 2902, 3042
Alw44I GTGCAC 1 cut(s) 2637
AlwI GGATC 9 cut(s) 115, 804, 817, 1295, 1308, 2099, 2792, 2833, 3198
AlwNI CAGNNNCTG 2 cut(s) 605, 752
Ama87I CYCGRG 1 cut(s) 609
AoxI GGCC 3 cut(s) 413, 976, 3180
ApaLI GTGCAC 1 cut(s) 2637
ApoI RAATTY 6 cut(s) 31, 584, 1165, 1183, 1345, 2715
AseI ATTAAT 1 cut(s) 165
AspLEI GCGC 1 cut(s) 3201
AspS9I GGNCC 4 cut(s) 413, 729, 976, 2693
AvaI CYCGRG 1 cut(s) 609
AvaII GGWCC 2 cut(s) 729, 2693
BaeGI GKGCMC 2 cut(s) 660, 2641
BamHI GGATCC 2 cut(s) 809, 1300
BanI GGYRCC 1 cut(s) 657
BanII GRGCYC 2 cut(s) 76, 310
BbrPI CACGTG 1 cut(s) 2027
Bbv12I GWGCWC 3 cut(s) 310, 1637, 2641
BccI CCATC 9 cut(s) 269, 282, 1097, 1265, 1412, 2312, 2584, 2825, 2992
BceAI ACGGC 1 cut(s) 1229
BcgI CGANNNNNNTGC 4 cut(s) 1256, 1290, 2375, 2409
BciT130I CCWGG 3 cut(s) 211, 805, 2403
BcoDI GTCTC 7 cut(s) 191, 1162, 1952, 2273, 2739, 2902, 3042
BfaI CTAG 9 cut(s) 134, 270, 725, 1023, 1161, 1595, 1889, 2243, 2787
BfmI CTRYAG 9 cut(s) 456, 753, 906, 1236, 1362, 1395, 1722, 2153, 2517
BfoI RGCGCY 1 cut(s) 3202
BfuAI ACCTGC 1 cut(s) 700
BglI GCCNNNNNGGC 1 cut(s) 984
BglII AGATCT 2 cut(s) 82, 1612
BlpI GCTNAGC 1 cut(s) 2259
BmcAI AGTACT 1 cut(s) 1373
Bme1390I CCNGG 3 cut(s) 211, 805, 2403
Bme18I GGWCC 2 cut(s) 729, 2693
BmeT110I CYCGRG 1 cut(s) 609
BmgBI CACGTC 1 cut(s) 2981
BmgT120I GGNCC 4 cut(s) 413, 729, 976, 2693
BmrFI CCNGG 3 cut(s) 211, 805, 2403
BmrI ACTGGG 2 cut(s) 1068, 2870
BmsI GCATC 8 cut(s) 256, 532, 1016, 1304, 2157, 2306, 2785, 3130
BmuI ACTGGG 2 cut(s) 1068, 2870
BpmI CTGGAG 4 cut(s) 232, 332, 392, 1890
Bpu1102I GCTNAGC 1 cut(s) 2259
BpuEI CTTGAG 1 cut(s) 2650
BsaAI YACGTR 1 cut(s) 2027
BsaBI GATNNNNATC 3 cut(s) 1305, 1611, 2109
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 7 cut(s) 704, 804, 1931, 2393, 2484, 2802, 2945
BsaWI WCCGGW 1 cut(s) 3130
BsaXI ACNNNNNCTCC 2 cut(s) 1927, 1957
Bsc4I CCNNNNNNNGG 4 cut(s) 234, 2114, 2702, 3172
Bse3DI GCAATG 1 cut(s) 2388
Bse8I GATNNNNATC 3 cut(s) 1305, 1611, 2109
BseBI CCWGG 3 cut(s) 211, 805, 2403
BseDI CCNNGG 7 cut(s) 704, 804, 1931, 2393, 2484, 2802, 2945
BseGI GGATG 8 cut(s) 10, 280, 293, 995, 1423, 1531, 2997, 3145
BseJI GATNNNNATC 3 cut(s) 1305, 1611, 2109
BseLI CCNNNNNNNGG 4 cut(s) 234, 2114, 2702, 3172
BseMI GCAATG 1 cut(s) 2388
BseMII CTCAG 5 cut(s) 1650, 2250, 2478, 2898, 3084
BseRI GAGGAG 8 cut(s) 183, 627, 657, 693, 2006, 2374, 2658, 2955
BseSI GKGCMC 2 cut(s) 660, 2641
BseYI CCCAGC 1 cut(s) 3062
BshFI GGCC 3 cut(s) 415, 978, 3182
BshNI GGYRCC 1 cut(s) 657
BsiHKAI GWGCWC 3 cut(s) 310, 1637, 2641
BsiHKCI CYCGRG 1 cut(s) 609
BsiSI CCGG 1 cut(s) 3131
BslFI GGGAC 3 cut(s) 715, 2522, 3008
BslI CCNNNNNNNGG 4 cut(s) 234, 2114, 2702, 3172
BsmAI GTCTC 7 cut(s) 191, 1162, 1952, 2273, 2739, 2902, 3042
BsmBI CGTCTC 2 cut(s) 1952, 2739
BsmFI GGGAC 3 cut(s) 715, 2522, 3008
BsnI GGCC 3 cut(s) 415, 978, 3182
Bso31I GGTCTC 1 cut(s) 191
BsoBI CYCGRG 1 cut(s) 609
Bsp1286I GDGCHC 5 cut(s) 76, 310, 660, 1637, 2641
Bsp1407I TGTACA 2 cut(s) 97, 1657
Bsp1720I GCTNAGC 1 cut(s) 2259
Bsp19I CCATGG 2 cut(s) 1931, 2484
BspANI GGCC 3 cut(s) 415, 978, 3182
BspCNI CTCAG 5 cut(s) 1649, 2251, 2477, 2897, 3083
BspHI TCATGA 1 cut(s) 550
BspMAI CTGCAG 6 cut(s) 757, 910, 1240, 1366, 1726, 2157
BspMI ACCTGC 1 cut(s) 700
BspPI GGATC 9 cut(s) 115, 804, 817, 1295, 1308, 2099, 2792, 2833, 3198
BspQI GCTCTTC 1 cut(s) 552
BspT107I GGYRCC 1 cut(s) 657
BspTNI GGTCTC 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 2388
BsrGI TGTACA 2 cut(s) 97, 1657
BssECI CCNNGG 7 cut(s) 704, 804, 1931, 2393, 2484, 2802, 2945
BssNAI GTATAC 1 cut(s) 341
BssT1I CCWWGG 4 cut(s) 1931, 2484, 2802, 2945
Bst1107I GTATAC 1 cut(s) 341
Bst2UI CCWGG 3 cut(s) 211, 805, 2403
Bst4CI ACNGT 5 cut(s) 772, 1849, 1943, 2587, 3044
Bst6I CTCTTC 6 cut(s) 143, 201, 552, 634, 2742, 3109
BstAPI GCANNNNNTGC 3 cut(s) 254, 605, 752
BstAUI TGTACA 2 cut(s) 97, 1657
BstBAI YACGTR 1 cut(s) 2027
BstC8I GCNNGC 3 cut(s) 980, 1476, 2615
BstDSI CCRYGG 2 cut(s) 1931, 2484
BstF5I GGATG 8 cut(s) 10, 280, 293, 995, 1423, 1531, 2997, 3145
BstH2I RGCGCY 1 cut(s) 3202
BstHHI GCGC 1 cut(s) 3201
BstMAI GTCTC 7 cut(s) 191, 1162, 1952, 2273, 2739, 2902, 3042
BstNI CCWGG 3 cut(s) 211, 805, 2403
BstNSI RCATGY 3 cut(s) 47, 1414, 1633
BstSCI CCNGG 3 cut(s) 209, 803, 2401
BstSFI CTRYAG 9 cut(s) 456, 753, 906, 1236, 1362, 1395, 1722, 2153, 2517
BstSLI GKGCMC 2 cut(s) 660, 2641
BstX2I RGATCY 7 cut(s) 82, 809, 1300, 1612, 2797, 2838, 3190
BstXI CCANNNNNNTGG 1 cut(s) 1555
BstYI RGATCY 7 cut(s) 82, 809, 1300, 1612, 2797, 2838, 3190
BstZ17I GTATAC 1 cut(s) 341
BsuRI GGCC 3 cut(s) 415, 978, 3182
BtgI CCRYGG 2 cut(s) 1931, 2484
BtgZI GCGATG 2 cut(s) 1187, 2803
BtrI CACGTC 1 cut(s) 2981
BtsCI GGATG 8 cut(s) 10, 280, 293, 995, 1423, 1531, 2997, 3145
BtsI GCAGTG 2 cut(s) 603, 921
BtsIMutI CAGTG 6 cut(s) 356, 368, 603, 768, 921, 2912
BveI ACCTGC 1 cut(s) 700
Cac8I GCNNGC 3 cut(s) 980, 1476, 2615
CaiI CAGNNNCTG 2 cut(s) 605, 752
CciI TCATGA 1 cut(s) 550
CfoI GCGC 1 cut(s) 3201
Cfr13I GGNCC 4 cut(s) 413, 729, 976, 2693
CseI GACGC 1 cut(s) 2721
Csp6I GTAC 9 cut(s) 98, 993, 1247, 1372, 1467, 1658, 1688, 2888, 2937
CviQI GTAC 9 cut(s) 98, 993, 1247, 1372, 1467, 1658, 1688, 2888, 2937
Eam1104I CTCTTC 6 cut(s) 143, 201, 552, 634, 2742, 3109
EarI CTCTTC 6 cut(s) 143, 201, 552, 634, 2742, 3109
Ecl136II GAGCTC 1 cut(s) 308
Eco130I CCWWGG 4 cut(s) 1931, 2484, 2802, 2945
Eco24I GRGCYC 2 cut(s) 76, 310
Eco31I GGTCTC 1 cut(s) 191
Eco32I GATATC 2 cut(s) 408, 2195
Eco47I GGWCC 2 cut(s) 729, 2693
Eco53kI GAGCTC 1 cut(s) 308
Eco57I CTGAAG 2 cut(s) 432, 2940
Eco72I CACGTG 1 cut(s) 2027
Eco88I CYCGRG 1 cut(s) 609
EcoICRI GAGCTC 1 cut(s) 308
EcoO109I RGGNCCY 2 cut(s) 729, 2693
EcoRII CCWGG 3 cut(s) 209, 803, 2401
EcoRV GATATC 2 cut(s) 408, 2195
EcoT14I CCWWGG 4 cut(s) 1931, 2484, 2802, 2945
EcoT38I GRGCYC 2 cut(s) 76, 310
ErhI CCWWGG 4 cut(s) 1931, 2484, 2802, 2945
Esp3I CGTCTC 2 cut(s) 1952, 2739
FalI AAGNNNNNCTT 2 cut(s) 2555, 2587
FaqI GGGAC 3 cut(s) 715, 2522, 3008
FauI CCCGC 2 cut(s) 1467, 2606
FblI GTMKAC 1 cut(s) 340
FokI GGATG 8 cut(s) 17, 287, 300, 982, 1430, 1518, 2984, 3152
FriOI GRGCYC 2 cut(s) 76, 310
FspBI CTAG 9 cut(s) 134, 270, 725, 1023, 1161, 1595, 1889, 2243, 2787
GlaI GCGC 1 cut(s) 3200
GsaI CCCAGC 1 cut(s) 3066
GsuI CTGGAG 4 cut(s) 232, 332, 392, 1890
HaeII RGCGCY 1 cut(s) 3202
HaeIII GGCC 3 cut(s) 415, 978, 3182
HapII CCGG 1 cut(s) 3131
HgaI GACGC 1 cut(s) 2721
HhaI GCGC 1 cut(s) 3201
Hin6I GCGC 1 cut(s) 3199
HinP1I GCGC 1 cut(s) 3199
HindIII AAGCTT 2 cut(s) 1693, 2222
HpaII CCGG 1 cut(s) 3131
Hpy188I TCNGA 7 cut(s) 405, 937, 1300, 1586, 1639, 1927, 2467
Hpy99I CGWCG 2 cut(s) 738, 1947
HpyCH4III ACNGT 5 cut(s) 772, 1849, 1943, 2587, 3044
HpyCH4IV ACGT 3 cut(s) 716, 2026, 2980
HpySE526I ACGT 3 cut(s) 716, 2026, 2980
HspAI GCGC 1 cut(s) 3199
KflI GGGWCCC 1 cut(s) 729
LguI GCTCTTC 1 cut(s) 552
LmnI GCTCC 7 cut(s) 71, 1221, 1738, 1871, 1935, 2671, 2773
LweI GCATC 8 cut(s) 256, 532, 1016, 1304, 2157, 2306, 2785, 3130
MaeI CTAG 9 cut(s) 134, 270, 725, 1023, 1161, 1595, 1889, 2243, 2787
MaeII ACGT 3 cut(s) 716, 2026, 2980
MaeIII GTNAC 9 cut(s) 451, 601, 712, 1135, 1387, 1458, 2027, 2741, 3073
MfeI CAATTG 1 cut(s) 1446
MflI RGATCY 7 cut(s) 82, 809, 1300, 1612, 2797, 2838, 3190
MhlI GDGCHC 5 cut(s) 76, 310, 660, 1637, 2641
MlyI GAGTC 5 cut(s) 371, 2531, 2549, 2642, 3095
MmeI TCCRAC 4 cut(s) 837, 1023, 1982, 2470
MseI TTAA 8 cut(s) 165, 1511, 1697, 2217, 2763, 2769, 3216, 3238
MslI CAYNNNNRTG 2 cut(s) 842, 3002
MspA1I CMGCKG 4 cut(s) 251, 681, 3062, 3120
MspI CCGG 1 cut(s) 3131
MspR9I CCNGG 3 cut(s) 211, 805, 2403
MunI CAATTG 1 cut(s) 1446
MvaI CCWGG 3 cut(s) 211, 805, 2403
NcoI CCATGG 2 cut(s) 1931, 2484
NmeAIII GCCGAG 1 cut(s) 685
NmuCI GTSAC 4 cut(s) 601, 2027, 2741, 3073
NspI RCATGY 3 cut(s) 47, 1414, 1633
OliI CACNNNNGTG 1 cut(s) 842
PaeR7I CTCGAG 1 cut(s) 609
PagI TCATGA 1 cut(s) 550
PciI ACATGT 2 cut(s) 1410, 1629
PciSI GCTCTTC 1 cut(s) 552
PfeI GAWTC 8 cut(s) 125, 281, 554, 1642, 1670, 1775, 2081, 2451
PfoI TCCNGGA 1 cut(s) 2401
PleI GAGTC 5 cut(s) 371, 2530, 2549, 2642, 3095
PmaCI CACGTG 1 cut(s) 2027
PmlI CACGTG 1 cut(s) 2027
PpsI GAGTC 5 cut(s) 371, 2530, 2549, 2642, 3095
Ppu21I YACGTR 1 cut(s) 2027
PpuMI RGGWCCY 2 cut(s) 729, 2693
PscI ACATGT 2 cut(s) 1410, 1629
PshBI ATTAAT 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 310
Psp5II RGGWCCY 2 cut(s) 729, 2693
Psp6I CCWGG 3 cut(s) 209, 803, 2401
PspCI CACGTG 1 cut(s) 2027
PspFI CCCAGC 1 cut(s) 3062
PspGI CCWGG 3 cut(s) 209, 803, 2401
PspPI GGNCC 4 cut(s) 413, 729, 976, 2693
PspPPI RGGWCCY 2 cut(s) 729, 2693
PspXI VCTCGAGB 1 cut(s) 609
PsrI GAACNNNNNNTAC 2 cut(s) 859, 891
PstI CTGCAG 6 cut(s) 757, 910, 1240, 1366, 1726, 2157
PstNI CAGNNNCTG 2 cut(s) 605, 752
PsuI RGATCY 7 cut(s) 82, 809, 1300, 1612, 2797, 2838, 3190
PvuII CAGCTG 2 cut(s) 251, 3062
RsaI GTAC 9 cut(s) 99, 994, 1248, 1373, 1468, 1659, 1689, 2889, 2938
RsaNI GTAC 9 cut(s) 98, 993, 1247, 1372, 1467, 1658, 1688, 2888, 2937
RseI CAYNNNNRTG 2 cut(s) 842, 3002
SacI GAGCTC 1 cut(s) 310
SapI GCTCTTC 1 cut(s) 552
SaqAI TTAA 8 cut(s) 165, 1511, 1697, 2217, 2763, 2769, 3216, 3238
Sau96I GGNCC 4 cut(s) 413, 729, 976, 2693
ScaI AGTACT 1 cut(s) 1373
SchI GAGTC 5 cut(s) 371, 2531, 2549, 2642, 3095
ScrFI CCNGG 3 cut(s) 211, 805, 2403
SduI GDGCHC 5 cut(s) 76, 310, 660, 1637, 2641
SfaNI GCATC 8 cut(s) 256, 532, 1016, 1304, 2157, 2306, 2785, 3130
SfcI CTRYAG 9 cut(s) 456, 753, 906, 1236, 1362, 1395, 1722, 2153, 2517
Sfr274I CTCGAG 1 cut(s) 609
SinI GGWCC 2 cut(s) 729, 2693
SlaI CTCGAG 1 cut(s) 609
SmiMI CAYNNNNRTG 2 cut(s) 842, 3002
SmlI CTYRAG 2 cut(s) 609, 2629
SmoI CTYRAG 2 cut(s) 609, 2629
SspI AATATT 2 cut(s) 163, 1037
SspMI CTAG 9 cut(s) 134, 270, 725, 1023, 1161, 1595, 1889, 2243, 2787
SstI GAGCTC 1 cut(s) 310
StyD4I CCNGG 3 cut(s) 209, 803, 2401
StyI CCWWGG 4 cut(s) 1931, 2484, 2802, 2945
TaaI ACNGT 5 cut(s) 772, 1849, 1943, 2587, 3044
TaiI ACGT 3 cut(s) 719, 2029, 2983
TaqI TCGA 5 cut(s) 610, 887, 1129, 2134, 3171
TatI WGTACW 4 cut(s) 97, 1371, 1657, 2936
TfiI GAWTC 8 cut(s) 125, 281, 554, 1642, 1670, 1775, 2081, 2451
Tru1I TTAA 8 cut(s) 165, 1511, 1697, 2217, 2763, 2769, 3216, 3238
Tru9I TTAA 8 cut(s) 165, 1511, 1697, 2217, 2763, 2769, 3216, 3238
TscAI CASTG 6 cut(s) 356, 375, 610, 775, 928, 2919
TseFI GTSAC 4 cut(s) 601, 2027, 2741, 3073
Tsp45I GTSAC 4 cut(s) 601, 2027, 2741, 3073
TspDTI ATGAA 7 cut(s) 188, 539, 1190, 1605, 1623, 2937, 3200
TspGWI ACGGA 3 cut(s) 457, 1675, 1840
TspRI CASTG 6 cut(s) 356, 375, 610, 775, 928, 2919
VneI GTGCAC 1 cut(s) 2637
VpaK11BI GGWCC 2 cut(s) 729, 2693
VspI ATTAAT 1 cut(s) 165
XapI RAATTY 6 cut(s) 31, 584, 1165, 1183, 1345, 2715
XbaI TCTAGA 2 cut(s) 1160, 2242
XceI RCATGY 3 cut(s) 47, 1414, 1633
XcmI CCANNNNNNNNNTGG 1 cut(s) 986
XhoI CTCGAG 1 cut(s) 609
XmiI GTMKAC 1 cut(s) 340
XspI CTAG 9 cut(s) 134, 270, 725, 1023, 1161, 1595, 1889, 2243, 2787
ZrmI AGTACT 1 cut(s) 1373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.