FvH4_2g00450

cheY-homologous receiver domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016556
Physical Location & Seq
Forward (+)
534278 .. 538572
4295 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g00450.t1

Sequence Viewer

Length: 522 bp
ATGGCCGAGGAAAGAGTTGTCCATGTTCTGGTCGTCGATGACTCCTTGGTTGACCGCACCATAGTTCACAAGCTCCTCAAAGCCTCCGCCTTTAAAGTTACTACTGTAGAGAGTGGGAAGAAAGCGCTAGAGGTTCTTGGAATGGAAGAAGAGAAGCTTGACAAGCCAACTGTTAAAGATCATGATGTTGACATAATCCTCACTGACTATTGTATGCCTGAGATGAATGGGCATGATCTTCTAGTTGCTATAAAGGATGACAGCCACATGAAATCAATTCCGGTGGTCATAGTGTCTTCTGAATTTAATCCACAGAGGATCAACAGGTGTCTCACAGATGGTGCTGAAGAGTTCTTGCAGAAACCTCTGAAAGTGAAAGACTTGGAGAAGCTAAGAAGCTATGTAAATCCAGCAGTTCCAGTACCCAAAATAGGTACAAAACAAAAAGCCGTAGTTGATCTGATGCCAGAAAGTAATGAAGCGGAAAGACTGCCCCACCTTGCTGGAGTTGCTGTTGCATAA

Protein Analysis

174

Amino Acids

19.2

Weight (kDa)

5.53

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Response_reg PF00072 9 - 130 1.8e-20 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016556)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00450 FvH4_2g00450
rosa_chinensis RchiOBHm_Chr6g0250401
rosa_laevigata RLG00000015542
rosa_multiflora Rmu_sc0000411.1_g000003 Rmu_sc0001209.1_g000037
rosa_roxburghii Rroxscaffold_7G00217260
rosa_rugosa Rorug05G0496100 Rorug05G0496200 Rorug05G0496300
rosa_samantha Rh6AG005700 Rh6BG005000
rosa_wichuraiana Rw6G000630 Rw6G000700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 28
AciI CCGC 3 cut(s) 55, 87, 482
AclWI GGATC 1 cut(s) 326
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 302
AcuI CTGAAG 1 cut(s) 366
AfaI GTAC 2 cut(s) 423, 436
AfeI AGCGCT 1 cut(s) 126
AfiI CCNNNNNNNGG 2 cut(s) 28, 431
AluBI AGCT 4 cut(s) 73, 157, 391, 399
AluI AGCT 4 cut(s) 73, 157, 391, 399
Alw26I GTCTC 1 cut(s) 335
AlwI GGATC 1 cut(s) 326
Aor51HI AGCGCT 1 cut(s) 126
AoxI GGCC 1 cut(s) 3
ApoI RAATTY 1 cut(s) 302
AspLEI GCGC 1 cut(s) 127
BbsI GAAGAC 1 cut(s) 288
BccI CCATC 1 cut(s) 332
BceAI ACGGC 1 cut(s) 434
BcoDI GTCTC 1 cut(s) 335
BfaI CTAG 2 cut(s) 128, 242
BfmI CTRYAG 1 cut(s) 105
BfoI RGCGCY 1 cut(s) 128
BmsI GCATC 1 cut(s) 453
BpiI GAAGAC 1 cut(s) 288
BsaJI CCNNGG 2 cut(s) 6, 45
BsaWI WCCGGW 1 cut(s) 280
BsaXI ACNNNNNCTCC 1 cut(s) 498
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 431
Bse1I ACTGG 1 cut(s) 419
BseDI CCNNGG 2 cut(s) 6, 45
BseGI GGATG 1 cut(s) 262
BseLI CCNNNNNNNGG 2 cut(s) 28, 431
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 1 cut(s) 419
BseRI GAGGAG 1 cut(s) 65
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 1 cut(s) 281
BslI CCNNNNNNNGG 2 cut(s) 28, 431
BsmAI GTCTC 1 cut(s) 335
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 4 cut(s) 178, 235, 318, 457
BspACI CCGC 3 cut(s) 55, 87, 482
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 211
BspHI TCATGA 1 cut(s) 181
BspPI GGATC 1 cut(s) 326
BsrI ACTGG 1 cut(s) 419
BssECI CCNNGG 2 cut(s) 6, 45
BssMI GATC 4 cut(s) 178, 235, 318, 457
BssT1I CCWWGG 1 cut(s) 45
Bst4CI ACNGT 2 cut(s) 106, 172
Bst6I CTCTTC 2 cut(s) 144, 342
BstDEI CTNAG 2 cut(s) 219, 392
BstF5I GGATG 1 cut(s) 262
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 1 cut(s) 127
BstKTI GATC 4 cut(s) 181, 238, 321, 460
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 4 cut(s) 178, 235, 318, 457
BstMWI GCNNNNNNNGC 2 cut(s) 163, 509
BstSFI CTRYAG 1 cut(s) 105
BstV2I GAAGAC 1 cut(s) 288
BstXI CCANNNNNNTGG 1 cut(s) 503
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 262
BtsIMutI CAGTG 1 cut(s) 201
CciI TCATGA 1 cut(s) 181
CfoI GCGC 1 cut(s) 127
Csp6I GTAC 2 cut(s) 422, 435
CspCI CAANNNNNGTGG 2 cut(s) 264, 299
CviAII CATG 4 cut(s) 23, 182, 233, 268
CviJI RGCY 9 cut(s) 5, 73, 83, 157, 166, 264, 391, 399, 449
CviKI_1 RGCY 9 cut(s) 5, 73, 83, 157, 166, 264, 391, 399, 449
CviQI GTAC 2 cut(s) 422, 435
DdeI CTNAG 2 cut(s) 219, 392
DpnI GATC 4 cut(s) 180, 237, 320, 459
DpnII GATC 4 cut(s) 178, 235, 318, 457
DraI TTTAAA 1 cut(s) 94
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 144, 342
EarI CTCTTC 2 cut(s) 144, 342
EciI GGCGGA 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 45
Eco47III AGCGCT 1 cut(s) 126
Eco57I CTGAAG 1 cut(s) 366
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 4 cut(s) 26, 185, 236, 271
FalI AAGNNNNNCTT 2 cut(s) 141, 173
FatI CATG 4 cut(s) 22, 181, 232, 267
FokI GGATG 1 cut(s) 269
FspBI CTAG 2 cut(s) 128, 242
GlaI GCGC 1 cut(s) 126
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 281
HhaI GCGC 1 cut(s) 127
Hin1II CATG 4 cut(s) 26, 185, 236, 271
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HincII GTYRAC 2 cut(s) 52, 190
HindII GTYRAC 2 cut(s) 52, 190
HindIII AAGCTT 1 cut(s) 155
HinfI GANTC 1 cut(s) 41
HpaII CCGG 1 cut(s) 281
Hpy166II GTNNAC 3 cut(s) 52, 67, 190
Hpy188I TCNGA 3 cut(s) 301, 369, 462
Hpy188III TCNNGA 1 cut(s) 182
Hpy8I GTNNAC 3 cut(s) 52, 67, 190
Hpy99I CGWCG 1 cut(s) 38
HpyCH4III ACNGT 2 cut(s) 106, 172
HpyCH4V TGCA 2 cut(s) 358, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 163, 509
HpyF3I CTNAG 2 cut(s) 219, 392
Hsp92II CATG 4 cut(s) 26, 185, 236, 271
HspAI GCGC 1 cut(s) 125
Kzo9I GATC 4 cut(s) 178, 235, 318, 457
LmnI GCTCC 1 cut(s) 78
LpnPI CCDG 8 cut(s) 14, 231, 294, 310, 423, 432, 480, 489
LweI GCATC 1 cut(s) 453
MaeI CTAG 2 cut(s) 128, 242
MaeIII GTNAC 1 cut(s) 97
MalI GATC 4 cut(s) 180, 237, 320, 459
MboI GATC 4 cut(s) 178, 235, 318, 457
MboII GAAGA 6 cut(s) 130, 158, 161, 230, 288, 359
MluCI AATT 2 cut(s) 276, 302
MlyI GAGTC 1 cut(s) 35
MnlI CCTC 6 cut(s) 86, 94, 124, 209, 309, 375
MseI TTAA 3 cut(s) 93, 174, 306
MspI CCGG 1 cut(s) 281
MwoI GCNNNNNNNGC 2 cut(s) 163, 509
NdeII GATC 4 cut(s) 178, 235, 318, 457
NlaIII CATG 4 cut(s) 26, 185, 236, 271
NmeAIII GCCGAG 1 cut(s) 31
PagI TCATGA 1 cut(s) 181
PflMI CCANNNNNTGG 1 cut(s) 28
PleI GAGTC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 35
RsaI GTAC 2 cut(s) 423, 436
RsaNI GTAC 2 cut(s) 422, 435
SaqAI TTAA 3 cut(s) 93, 174, 306
Sau3AI GATC 4 cut(s) 178, 235, 318, 457
SchI GAGTC 1 cut(s) 35
SetI ASST 9 cut(s) 75, 135, 159, 329, 367, 393, 401, 436, 501
SfaNI GCATC 1 cut(s) 453
SfcI CTRYAG 1 cut(s) 105
Sse9I AATT 2 cut(s) 276, 302
SsiI CCGC 3 cut(s) 55, 87, 482
SspMI CTAG 2 cut(s) 128, 242
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 2 cut(s) 106, 172
TaqI TCGA 1 cut(s) 36
TasI AATT 2 cut(s) 276, 302
Tru1I TTAA 3 cut(s) 93, 174, 306
Tru9I TTAA 3 cut(s) 93, 174, 306
TscAI CASTG 1 cut(s) 208
TspDTI ATGAA 3 cut(s) 239, 284, 492
TspRI CASTG 1 cut(s) 208
Van91I CCANNNNNTGG 1 cut(s) 28
XapI RAATTY 1 cut(s) 302
XspI CTAG 2 cut(s) 128, 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.