FvH4_2g04750

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013876
Physical Location & Seq
Reverse (-)
3685589 .. 3688170
2582 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g04750.t1

Sequence Viewer

Length: 1791 bp
ATGGCGGTCGACTCGTCCCTTCCTTCCCCTCTAGGGCCACCGGCCTGCGAGAAAGACGCCAAGGCTTTACAATTTATCGAACAAACGACTCGAAACGCCGCCGGCGTCCAGGAGAGGGTTTTGGCCGAGATTTTGAGCCGAAACGCCGAGACTGAGTATCTCCGACGCTACAAGCTCGGCGGCGCCACTGACCGCAAATCCTTCAAATCCAAGGTTCCCGTGATTACCTACGAGGATCTCCAGCCGGAAATCCAAAGGATCGCCAATGGAGACCGCTCTCCCATCCTCTCCGCTCATCCAATTTCTGAATTTCTCACCAGCTCTGGTACTTCAGCTGGTGAGAGGAAGCTGATGCCAACGATTCAGGAGGAGCTGGACCGTCGCCAATCACTCTACAATCTTCTCATGCCGGTCATGAACCTTTGTGTGCCTGGGTTGGACAAAGGCAAAGGCTTGTACTTTCTGTTCGTCAAGTCCGAATCAAAAACACCGGGCGGACTCTTGGCCCGACCCGTTTTGACCAGCTACTACAAAAGCGAGCATTTCAAGACCCGCCCTTACGACCCGTACAATGTCTACACCAGCCCAAACGAGGCCATCCTCTGCGCCGATTCTTTTCAATCCATGTACACTCAAATGCTCTGTGGTCTCCTCGAGCGCCACCAAGTCCTCCGCCTCGGCGCCGTTTTCGCCTCCGGTCTCCTCCGCGCCATCCGCTTCCTCCAGCTCAACTGGCAGCAACTGGCCGCCGATATCCGAACCGGAACACTCAACTCCAAAATTACCGACCCGGAGTTGAAAGAATGCATGGAGCGGACTCTCAAACCTGACTCCGAACTAGCCGAGTTCGTCGCCGAACAATGCGGGGAGGAGAATTGGGAGGGAATCATAACACGAATTTGGCCCAACACCAAGTACCTTGACGTCATCGTCACTGGAGCCATGGCGCAGTATATTCCGACGTTGGATTACTACAGCGGAGGGCTACCAATGGCATGCACAATGTATGCTTCTTCCGAGTGTTACTTCGGACTCAACCTCAACCCGATGTGCAAGCCTTCTGAAGTTTCGTACACAATCATGCCGAACATGGCCTACTTTGAGTTCCTCCCCTACGACCCCAACTCCATGGGTCTTGACCCGACCCGACTTGTCGACCTGGTCGACGTGGAGGTCGGAAGGGAATACGAGCTCGTAATTTCAACCTACGCCGGGCTCTACCGTTACCGCGTCGGCGACATTCTAAGAGTCACCGGGTTCCACAACTCGGCGCCGCAGTTCCACTTCGTGCGCCGCAAGAACGTCCTCCTCAGCATCGACTCCGACAAGACCGACGAGGCGGAGCTCCAGCTCGCCGTCGACAACGCCTCCAAGCTCGTACTCGAGAAGCTCAACGCCAGCGTCGTCGAGTACACCAGCTTCGCCGACACCAAGACCATCCCCGGCCACTACGTCATCTACTGGGAGCTTCTAGTCAAGGACTCGGCCGAGCCGCCCAGCTCGGAGTCGCTGAGTCAGTGCTGCTTGGCCATGGAGGAGTCGCTCAACTCGGTTTACCGGCAGTGCCGGGTGGCGGACAACTCGATCGGGCCGTTGGAGATTCGGGTGGTGAAGAGTGGCACGTTTGAGGAGTTGATGGACTACGCGATCTCGAGAGGCGCGTCGATAAACCAGTATAAGGTGCCGAGGTGTGTGAATTTTACCCCTATAATGGAGTTACTGGACTCGAGGGTGGTTTCCAAGCATTTTAGCCCGTCTTTACCGCACTGGACCCCGGAAAGGAGACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GH3

Protein Analysis

597

Amino Acids

67.17

Weight (kDa)

5.55

Isoelectric Point (pI)

46.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH3 PF03321 23 - 331 2e-122 GH3 family N-terminal domain
GH3_M PF23571 358 - 432 3e-30 GH3 family central domain
GH3_C PF23572 448 - 567 4.6e-40 GH3 family C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013876)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14960
fragaria_vesca FvH4_2g04750
malus_domestica MD05G1092300.v1.1 MD05G1092900.v1.1
prunus_persica Prupe.8G137900_v2.0.a1
pyrus_communis pycom05g09190 pycom05g09210
rosa_chinensis RchiOBHm_Chr6g0254401
rosa_laevigata RLG00000014915
rosa_multiflora Rmu_ssc0000481.1_g000001
rosa_roxburghii Rroxscaffold_7G00210830
rosa_rugosa Rorug05G0552100
rosa_samantha Rh6AG069100 Rh6BG061700 Rh6CG061900 Rh6DG058900
rosa_wichuraiana Rw6G006070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 929, 1172
AatII GACGTC 1 cut(s) 927
AccB1I GGYRCC 4 cut(s) 182, 680, 1270, 1681
AccBSI CCGCTC 3 cut(s) 276, 293, 814
AccI GTMKAC 5 cut(s) 9, 576, 1155, 1164, 1359
AccII CGCG 4 cut(s) 708, 1230, 1646, 1661
AclWI GGATC 2 cut(s) 243, 266
AcoI YGGCCR 5 cut(s) 123, 744, 1444, 1485, 1527
AcsI RAATTY 3 cut(s) 308, 897, 1696
AcuI CTGAAG 2 cut(s) 315, 1083
AcyI GRCGYC 6 cut(s) 57, 105, 183, 681, 924, 1271
AdeI CACNNNGTG 1 cut(s) 1288
AfaI GTAC 8 cut(s) 328, 458, 569, 629, 917, 1073, 1380, 1412
AfiI CCNNNNNNNGG 9 cut(s) 33, 115, 592, 1212, 1267, 1573, 1678, 1711, 1779
AgsI TTSAA 5 cut(s) 205, 547, 620, 799, 1203
AjiI CACGTC 1 cut(s) 1168
AjnI CCWGG 3 cut(s) 108, 430, 1158
Alw21I GWGCWC 2 cut(s) 1194, 1347
Alw26I GTCTC 5 cut(s) 143, 264, 653, 704, 1777
AlwI GGATC 2 cut(s) 243, 266
AlwNI CAGNNNCTG 1 cut(s) 742
Ama87I CYCGRG 4 cut(s) 653, 1382, 1651, 1726
ApeKI GCWGC 2 cut(s) 736, 1521
ApoI RAATTY 3 cut(s) 308, 897, 1696
AspLEI GCGC 9 cut(s) 185, 608, 660, 683, 710, 949, 1273, 1293, 1661
AspS9I GGNCC 6 cut(s) 35, 376, 505, 903, 1589, 1770
AsuC2I CCSGG 7 cut(s) 492, 791, 1213, 1255, 1443, 1568, 1775
AsuHPI GGTGA 4 cut(s) 307, 350, 1243, 1621
AvaI CYCGRG 4 cut(s) 653, 1382, 1651, 1726
AvaII GGWCC 2 cut(s) 376, 1770
BalI TGGCCA 1 cut(s) 1529
BanI GGYRCC 4 cut(s) 182, 680, 1270, 1681
BanII GRGCYC 3 cut(s) 1194, 1218, 1347
BarI GAAGNNNNNNTAC 2 cut(s) 1403, 1435
Bbv12I GWGCWC 2 cut(s) 1194, 1347
BbvCI CCTCAGC 1 cut(s) 1310
BbvI GCAGC 2 cut(s) 748, 1508
BccI CCATC 5 cut(s) 290, 605, 719, 1445, 1630
BceAI ACGGC 3 cut(s) 668, 1340, 1576
BciT130I CCWGG 3 cut(s) 110, 432, 1160
BcnI CCSGG 7 cut(s) 492, 791, 1213, 1255, 1443, 1568, 1775
BcoDI GTCTC 5 cut(s) 143, 264, 653, 704, 1777
BfaI CTAG 3 cut(s) 32, 839, 1472
BfmI CTRYAG 1 cut(s) 973
BfoI RGCGCY 4 cut(s) 186, 661, 684, 1274
BisI GCNGC 8 cut(s) 99, 181, 737, 747, 1274, 1294, 1493, 1522
BlsI GCNGC 8 cut(s) 100, 182, 738, 748, 1275, 1295, 1494, 1523
Bme18I GGWCC 2 cut(s) 376, 1770
BmeT110I CYCGRG 4 cut(s) 653, 1382, 1651, 1726
BmgBI CACGTC 1 cut(s) 1168
BmgT120I GGNCC 6 cut(s) 35, 376, 505, 903, 1589, 1770
BmiI GGNNCC 8 cut(s) 184, 216, 682, 940, 1259, 1272, 1683, 1772
BmrI ACTGGG 1 cut(s) 1471
BmsI GCATC 2 cut(s) 342, 1323
BmuI ACTGGG 1 cut(s) 1471
BpmI CTGGAG 4 cut(s) 224, 707, 957, 1331
Bpu10I CCTNAGC 1 cut(s) 1310
BpuMI CCSGG 7 cut(s) 492, 791, 1213, 1255, 1443, 1568, 1775
BsaHI GRCGYC 6 cut(s) 57, 105, 183, 681, 924, 1271
BsaI GGTCTC 3 cut(s) 264, 653, 704
BsaWI WCCGGW 2 cut(s) 695, 761
BsaXI ACNNNNNCTCC 4 cut(s) 1109, 1139, 1352, 1382
Bsc4I CCNNNNNNNGG 9 cut(s) 33, 115, 592, 1212, 1267, 1573, 1678, 1711, 1779
Bse118I RCCGGY 4 cut(s) 40, 101, 409, 1557
Bse1I ACTGG 7 cut(s) 737, 747, 940, 1466, 1672, 1725, 1772
BseBI CCWGG 3 cut(s) 110, 432, 1160
BseGI GGATG 5 cut(s) 282, 295, 597, 711, 1437
BseLI CCNNNNNNNGG 9 cut(s) 33, 115, 592, 1212, 1267, 1573, 1678, 1711, 1779
BseMII CTCAG 3 cut(s) 144, 1324, 1502
BseNI ACTGG 7 cut(s) 737, 747, 940, 1466, 1672, 1725, 1772
BseRI GAGGAG 7 cut(s) 383, 641, 692, 884, 1298, 1550, 1643
BseX3I CGGCCG 1 cut(s) 1485
BseXI GCAGC 2 cut(s) 748, 1508
BseYI CCCAGC 1 cut(s) 1496
Bsh1236I CGCG 4 cut(s) 708, 1230, 1646, 1661
Bsh1285I CGRYCG 3 cut(s) 9, 1488, 1587
BshNI GGYRCC 4 cut(s) 182, 680, 1270, 1681
BsiEI CGRYCG 3 cut(s) 9, 1488, 1587
BsiHKAI GWGCWC 2 cut(s) 1194, 1347
BsiHKCI CYCGRG 4 cut(s) 653, 1382, 1651, 1726
BslI CCNNNNNNNGG 9 cut(s) 33, 115, 592, 1212, 1267, 1573, 1678, 1711, 1779
BsmAI GTCTC 5 cut(s) 143, 264, 653, 704, 1777
BsmBI CGTCTC 1 cut(s) 1777
BsmI GAATGC 1 cut(s) 809
Bso31I GGTCTC 3 cut(s) 264, 653, 704
BsoBI CYCGRG 4 cut(s) 653, 1382, 1651, 1726
Bsp1286I GDGCHC 3 cut(s) 1194, 1218, 1347
Bsp1407I TGTACA 1 cut(s) 627
Bsp143I GATC 4 cut(s) 235, 258, 1584, 1647
Bsp19I CCATGG 3 cut(s) 942, 1128, 1530
BspCNI CTCAG 3 cut(s) 145, 1323, 1503
BspFNI CGCG 4 cut(s) 708, 1230, 1646, 1661
BspHI TCATGA 1 cut(s) 414
BspLI GGNNCC 8 cut(s) 184, 216, 682, 940, 1259, 1272, 1683, 1772
BspPI GGATC 2 cut(s) 243, 266
BspT107I GGYRCC 4 cut(s) 182, 680, 1270, 1681
BspTNI GGTCTC 3 cut(s) 264, 653, 704
BsrBI CCGCTC 3 cut(s) 276, 293, 814
BsrFI RCCGGY 4 cut(s) 40, 101, 409, 1557
BsrGI TGTACA 1 cut(s) 627
BsrI ACTGG 7 cut(s) 737, 747, 940, 1466, 1672, 1725, 1772
BssAI RCCGGY 4 cut(s) 40, 101, 409, 1557
BssMI GATC 4 cut(s) 235, 258, 1584, 1647
BssNI GRCGYC 6 cut(s) 57, 105, 183, 681, 924, 1271
BssT1I CCWWGG 5 cut(s) 60, 210, 942, 1128, 1530
Bst2UI CCWGG 3 cut(s) 110, 432, 1160
Bst4CI ACNGT 3 cut(s) 380, 1223, 1788
Bst6I CTCTTC 1 cut(s) 1607
BstACI GRCGYC 6 cut(s) 57, 105, 183, 681, 924, 1271
BstAUI TGTACA 1 cut(s) 627
BstC8I GCNNGC 7 cut(s) 46, 103, 539, 997, 1055, 1353, 1399
BstDEI CTNAG 4 cut(s) 153, 1244, 1310, 1511
BstDSI CCRYGG 3 cut(s) 942, 1128, 1530
BstF5I GGATG 5 cut(s) 282, 295, 597, 711, 1437
BstFNI CGCG 4 cut(s) 708, 1230, 1646, 1661
BstH2I RGCGCY 4 cut(s) 186, 661, 684, 1274
BstHHI GCGC 9 cut(s) 185, 608, 660, 683, 710, 949, 1273, 1293, 1661
BstKTI GATC 4 cut(s) 238, 261, 1587, 1650
BstMAI GTCTC 5 cut(s) 143, 264, 653, 704, 1777
BstMBI GATC 4 cut(s) 235, 258, 1584, 1647
BstMCI CGRYCG 3 cut(s) 9, 1488, 1587
BstMWI GCNNNNNNNGC 3 cut(s) 689, 714, 733
BstNI CCWGG 3 cut(s) 110, 432, 1160
BstNSI RCATGY 1 cut(s) 999
BstSFI CTRYAG 1 cut(s) 973
BstUI CGCG 4 cut(s) 708, 1230, 1646, 1661
BstV1I GCAGC 2 cut(s) 748, 1508
BstX2I RGATCY 1 cut(s) 235
BstXI CCANNNNNNTGG 1 cut(s) 1129
BstYI RGATCY 1 cut(s) 235
BstZI CGGCCG 1 cut(s) 1485
BtgI CCRYGG 3 cut(s) 942, 1128, 1530
BtrI CACGTC 1 cut(s) 1168
BtsCI GGATG 5 cut(s) 282, 295, 597, 711, 1437
BtsI GCAGTG 1 cut(s) 1568
BtsIMutI CAGTG 5 cut(s) 186, 933, 1523, 1568, 1765
Cac8I GCNNGC 7 cut(s) 46, 103, 539, 997, 1055, 1353, 1399
CaiI CAGNNNCTG 1 cut(s) 742
CciI TCATGA 1 cut(s) 414
CfoI GCGC 9 cut(s) 185, 608, 660, 683, 710, 949, 1273, 1293, 1661
Cfr10I RCCGGY 4 cut(s) 40, 101, 409, 1557
Cfr13I GGNCC 6 cut(s) 35, 376, 505, 903, 1589, 1770
CseI GACGC 6 cut(s) 65, 94, 174, 1219, 1390, 1650
CsiI ACCWGGT 1 cut(s) 1158
Csp6I GTAC 8 cut(s) 327, 457, 568, 628, 916, 1072, 1379, 1411
CviQI GTAC 8 cut(s) 327, 457, 568, 628, 916, 1072, 1379, 1411
DdeI CTNAG 4 cut(s) 153, 1244, 1310, 1511
DinI GGCGCC 3 cut(s) 184, 682, 1272
DpnI GATC 4 cut(s) 237, 260, 1586, 1649
DpnII GATC 4 cut(s) 235, 258, 1584, 1647
DraIII CACNNNGTG 1 cut(s) 1288
DrdI GACNNNNNNGTC 2 cut(s) 929, 1172
DseDI GACNNNNNNGTC 2 cut(s) 929, 1172
EaeI YGGCCR 5 cut(s) 123, 744, 1444, 1485, 1527
EagI CGGCCG 1 cut(s) 1485
Eam1104I CTCTTC 1 cut(s) 1607
EarI CTCTTC 1 cut(s) 1607
EciI GGCGGA 4 cut(s) 510, 662, 1355, 1589
Ecl136II GAGCTC 2 cut(s) 1192, 1345
EclXI CGGCCG 1 cut(s) 1485
Eco130I CCWWGG 5 cut(s) 60, 210, 942, 1128, 1530
Eco24I GRGCYC 3 cut(s) 1194, 1218, 1347
Eco31I GGTCTC 3 cut(s) 264, 653, 704
Eco32I GATATC 1 cut(s) 754
Eco47I GGWCC 2 cut(s) 376, 1770
Eco52I CGGCCG 1 cut(s) 1485
Eco53kI GAGCTC 2 cut(s) 1192, 1345
Eco57I CTGAAG 2 cut(s) 315, 1083
Eco88I CYCGRG 4 cut(s) 653, 1382, 1651, 1726
EcoICRI GAGCTC 2 cut(s) 1192, 1345
EcoRII CCWGG 3 cut(s) 108, 430, 1158
EcoRV GATATC 1 cut(s) 754
EcoT14I CCWWGG 5 cut(s) 60, 210, 942, 1128, 1530
EcoT22I ATGCAT 1 cut(s) 809
EcoT38I GRGCYC 3 cut(s) 1194, 1218, 1347
EgeI GGCGCC 3 cut(s) 184, 682, 1272
EheI GGCGCC 3 cut(s) 184, 682, 1272
ErhI CCWWGG 5 cut(s) 60, 210, 942, 1128, 1530
Esp3I CGTCTC 1 cut(s) 1777
FauI CCCGC 2 cut(s) 560, 857
FblI GTMKAC 5 cut(s) 9, 576, 1155, 1164, 1359
Fnu4HI GCNGC 8 cut(s) 99, 181, 737, 747, 1274, 1294, 1493, 1522
FokI GGATG 5 cut(s) 269, 282, 584, 698, 1424
FriOI GRGCYC 3 cut(s) 1194, 1218, 1347
Fsp4HI GCNGC 8 cut(s) 99, 181, 737, 747, 1274, 1294, 1493, 1522
FspBI CTAG 3 cut(s) 32, 839, 1472
GlaI GCGC 9 cut(s) 184, 607, 659, 682, 709, 948, 1272, 1292, 1660
GluI GCNGC 8 cut(s) 99, 181, 737, 747, 1274, 1294, 1493, 1522
GsaI CCCAGC 1 cut(s) 1500
GsuI CTGGAG 4 cut(s) 224, 707, 957, 1331
HaeII RGCGCY 4 cut(s) 186, 661, 684, 1274
HgaI GACGC 6 cut(s) 65, 94, 174, 1219, 1390, 1650
HhaI GCGC 9 cut(s) 185, 608, 660, 683, 710, 949, 1273, 1293, 1661
Hin1I GRCGYC 6 cut(s) 57, 105, 183, 681, 924, 1271
Hin6I GCGC 9 cut(s) 183, 606, 658, 681, 708, 947, 1271, 1291, 1659
HinP1I GCGC 9 cut(s) 183, 606, 658, 681, 708, 947, 1271, 1291, 1659
HincII GTYRAC 4 cut(s) 10, 1156, 1165, 1360
HindII GTYRAC 4 cut(s) 10, 1156, 1165, 1360
HphI GGTGA 4 cut(s) 307, 350, 1243, 1621
Hpy166II GTNNAC 9 cut(s) 10, 577, 630, 1074, 1156, 1165, 1360, 1413, 1555
Hpy188III TCNNGA 7 cut(s) 365, 415, 547, 1136, 1384, 1651, 1653
Hpy8I GTNNAC 9 cut(s) 10, 577, 630, 1074, 1156, 1165, 1360, 1413, 1555
HpyAV CCTTC 5 cut(s) 29, 33, 211, 1068, 1173
HpyCH4III ACNGT 3 cut(s) 380, 1223, 1788
HpyCH4IV ACGT 6 cut(s) 924, 962, 1167, 1302, 1452, 1622
HpyCH4V TGCA 3 cut(s) 807, 999, 1053
HpyF10VI GCNNNNNNNGC 3 cut(s) 689, 714, 733
HpyF3I CTNAG 4 cut(s) 153, 1244, 1310, 1511
HpySE526I ACGT 6 cut(s) 924, 962, 1167, 1302, 1452, 1622
Hsp92I GRCGYC 6 cut(s) 57, 105, 183, 681, 924, 1271
HspAI GCGC 9 cut(s) 183, 606, 658, 681, 708, 947, 1271, 1291, 1659
KasI GGCGCC 3 cut(s) 182, 680, 1270
KroI GCCGGC 1 cut(s) 101
KroNI GCCGGC 1 cut(s) 103
Kzo9I GATC 4 cut(s) 235, 258, 1584, 1647
LmnI GCTCC 6 cut(s) 370, 811, 938, 1342, 1350, 1465
Lsp1109I GCAGC 2 cut(s) 748, 1508
LweI GCATC 2 cut(s) 342, 1323
MabI ACCWGGT 1 cut(s) 1158
MaeI CTAG 3 cut(s) 32, 839, 1472
MaeII ACGT 6 cut(s) 924, 962, 1167, 1302, 1452, 1622
MaeIII GTNAC 5 cut(s) 931, 1022, 1223, 1249, 1716
MalI GATC 4 cut(s) 237, 260, 1586, 1649
MbiI CCGCTC 3 cut(s) 276, 293, 814
MboI GATC 4 cut(s) 235, 258, 1584, 1647
MboII GAAGA 3 cut(s) 392, 1005, 1624
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 3 cut(s) 1194, 1218, 1347
MlsI TGGCCA 1 cut(s) 1529
MluCI AATT 8 cut(s) 71, 300, 308, 780, 874, 897, 1197, 1696
MluNI TGGCCA 1 cut(s) 1529
Mly113I GGCGCC 3 cut(s) 183, 681, 1271
MmeI TCCRAC 7 cut(s) 187, 417, 945, 983, 1156, 1347, 1575
Mox20I TGGCCA 1 cut(s) 1529
Mph1103I ATGCAT 1 cut(s) 809
MreI CGCCGGCG 1 cut(s) 101
MroNI GCCGGC 1 cut(s) 101
MscI TGGCCA 1 cut(s) 1529
MslI CAYNNNNRTG 2 cut(s) 635, 1079
Msp20I TGGCCA 1 cut(s) 1529
MspA1I CMGCKG 2 cut(s) 335, 978
Mva1269I GAATGC 1 cut(s) 809
MvaI CCWGG 3 cut(s) 110, 432, 1160
MvnI CGCG 4 cut(s) 708, 1230, 1646, 1661
MwoI GCNNNNNNNGC 3 cut(s) 689, 714, 733
NaeI GCCGGC 1 cut(s) 103
NarI GGCGCC 3 cut(s) 183, 681, 1271
NciI CCSGG 7 cut(s) 492, 791, 1213, 1255, 1443, 1568, 1775
NcoI CCATGG 3 cut(s) 942, 1128, 1530
NdeII GATC 4 cut(s) 235, 258, 1584, 1647
NgoMIV GCCGGC 1 cut(s) 101
NlaIV GGNNCC 8 cut(s) 184, 216, 682, 940, 1259, 1272, 1683, 1772
NmeAIII GCCGAG 9 cut(s) 151, 156, 172, 657, 868, 1247, 1463, 1513, 1710
NmuCI GTSAC 2 cut(s) 931, 1249
NsiI ATGCAT 1 cut(s) 809
NspI RCATGY 1 cut(s) 999
PaeI GCATGC 1 cut(s) 999
PaeR7I CTCGAG 4 cut(s) 653, 1382, 1651, 1726
PagI TCATGA 1 cut(s) 414
PcsI WCGNNNNNNNCGW 6 cut(s) 102, 474, 1161, 1401, 1589, 1658
PctI GAATGC 1 cut(s) 809
PdiI GCCGGC 1 cut(s) 103
PfeI GAWTC 5 cut(s) 361, 479, 611, 885, 1600
PflFI GACNNNGTC 1 cut(s) 1160
PfoI TCCNGGA 1 cut(s) 108
PkrI GCNGC 8 cut(s) 100, 182, 738, 748, 1275, 1295, 1494, 1523
Ple19I CGATCG 1 cut(s) 1587
PluTI GGCGCC 3 cut(s) 186, 684, 1274
Psp124BI GAGCTC 2 cut(s) 1194, 1347
Psp6I CCWGG 3 cut(s) 108, 430, 1158
PspFI CCCAGC 1 cut(s) 1496
PspGI CCWGG 3 cut(s) 108, 430, 1158
PspN4I GGNNCC 8 cut(s) 184, 216, 682, 940, 1259, 1272, 1683, 1772
PspPI GGNCC 6 cut(s) 35, 376, 505, 903, 1589, 1770
PspXI VCTCGAGB 2 cut(s) 653, 1726
PsrI GAACNNNNNNTAC 4 cut(s) 449, 481, 1079, 1111
PstNI CAGNNNCTG 1 cut(s) 742
PsuI RGATCY 1 cut(s) 235
PsyI GACNNNGTC 1 cut(s) 1160
PvuI CGATCG 1 cut(s) 1587
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 8 cut(s) 328, 458, 569, 629, 917, 1073, 1380, 1412
RsaNI GTAC 8 cut(s) 327, 457, 568, 628, 916, 1072, 1379, 1411
RseI CAYNNNNRTG 2 cut(s) 635, 1079
SacI GAGCTC 2 cut(s) 1194, 1347
SalI GTCGAC 4 cut(s) 8, 1154, 1163, 1358
SatI GCNGC 8 cut(s) 99, 181, 737, 747, 1274, 1294, 1493, 1522
Sau3AI GATC 4 cut(s) 235, 258, 1584, 1647
Sau96I GGNCC 6 cut(s) 35, 376, 505, 903, 1589, 1770
SduI GDGCHC 3 cut(s) 1194, 1218, 1347
SexAI ACCWGGT 1 cut(s) 1158
SfaNI GCATC 2 cut(s) 342, 1323
SfcI CTRYAG 1 cut(s) 973
SfoI GGCGCC 3 cut(s) 184, 682, 1272
Sfr274I CTCGAG 4 cut(s) 653, 1382, 1651, 1726
SgrAI CRCCGGYG 1 cut(s) 101
SinI GGWCC 2 cut(s) 376, 1770
SlaI CTCGAG 4 cut(s) 653, 1382, 1651, 1726
SmiMI CAYNNNNRTG 2 cut(s) 635, 1079
SmlI CTYRAG 4 cut(s) 653, 1382, 1651, 1726
SmoI CTYRAG 4 cut(s) 653, 1382, 1651, 1726
SphI GCATGC 1 cut(s) 999
Sse9I AATT 8 cut(s) 71, 300, 308, 780, 874, 897, 1197, 1696
SspDI GGCGCC 3 cut(s) 182, 680, 1270
SspMI CTAG 3 cut(s) 32, 839, 1472
SstI GAGCTC 2 cut(s) 1194, 1347
StyI CCWWGG 5 cut(s) 60, 210, 942, 1128, 1530
TaaI ACNGT 3 cut(s) 380, 1223, 1788
TaiI ACGT 6 cut(s) 927, 965, 1170, 1305, 1455, 1625
TaqII GACCGA 1 cut(s) 1346
TasI AATT 8 cut(s) 71, 300, 308, 780, 874, 897, 1197, 1696
TatI WGTACW 3 cut(s) 456, 627, 1410
TauI GCSGC 6 cut(s) 101, 183, 749, 1276, 1296, 1495
TfiI GAWTC 5 cut(s) 361, 479, 611, 885, 1600
TscAI CASTG 5 cut(s) 193, 940, 1523, 1568, 1772
TseFI GTSAC 2 cut(s) 931, 1249
TseI GCWGC 2 cut(s) 736, 1521
Tsp45I GTSAC 2 cut(s) 931, 1249
TspDTI ATGAA 1 cut(s) 431
TspRI CASTG 5 cut(s) 193, 940, 1523, 1568, 1772
Tth111I GACNNNGTC 1 cut(s) 1160
VpaK11BI GGWCC 2 cut(s) 376, 1770
XapI RAATTY 3 cut(s) 308, 897, 1696
XceI RCATGY 1 cut(s) 999
XhoI CTCGAG 4 cut(s) 653, 1382, 1651, 1726
XmiI GTMKAC 5 cut(s) 9, 576, 1155, 1164, 1359
XspI CTAG 3 cut(s) 32, 839, 1472
ZraI GACGTC 1 cut(s) 925
Zsp2I ATGCAT 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.