Rh6AG069100

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0013876
Physical Location & Seq
Reverse (-)
10004572 .. 10007177
2606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG069100.1

Sequence Viewer

Length: 1491 bp
ATGGAAATTTTTGGTTACAGCTCTGGTACTTCAGCGGGTGAGAGGAAGCTGATGCCAACGATTCAGGAGGAGTTGGATCGTCGTCAATCACTCTACAACCTCCTCATGCCAGTCATGAACCTTTGTGTGCCTGGTTTGGACAAAGGAAAGGGCTTGTACTTTCTGTTCGTCAAGTCCGAATCAAAGACTCCGGGCGGACTCTTGGCCCGACCCGTTTTGACCAGCTACTACAAAAGCGAGCATTTCAAGACTCGCCCTTACGATCCCTACAATGTCTACACCAGCCCCAACGAGGCCATCCTCTGCCCCGACTCGTTTCAATCCATGTACACTCAAATGCTCTGTGGCCTCCTCGAGCGCCACCAAGTCCTCCGCCTCGGCGCCGTTTTCGCCTCCGGTCTCCTCCGCGCCATCCACTTCCTCCAGCTCAACTGGCAGCAACTCGCCGCCGACATCCGCACCGGAACACTCAACCCCAAAATTACCGACCCGGAGTTGAGGGAATGCATGGAGCGGAAACTGAAACCCGAACCTGAACTTGCTGAGTTCGTTGCCGAACAATGCGGCGAGGAGAATTGGCAGGGAATCATAACCCGAATTTGGCCCAGTACAAAATATCTCGATGTCATCGTCACCGGAGCTATGGCCCAGTATATTCCGACTTTGGATTACTACAGCGGCGGCTTACCCATGGCCTGCACCATGTACGCTTCGTCCGAGTGTTACTTCGGACTCAACCTGAACCCCATGTGCAGACCTTCAGAAGTTTCATACACAATTATGCCAAACATGGCTTACTTCGAGTTCCTCCCCTACGAGACCAACTCAGTGAGTCTTGACCCGACCCGACTCGTTGAACTGGTCGACGTGGTGGTCGGAAAAGAATACGAGCTCGTTATTACCACTTACGCCGGACTCTACCGCTACCGCGTCGGCGACATTCTCCGGGTCACCGGGTTCCACAACTCGGCGCCGCAGTTCCACTTCGTGCGGCGGAAGAACGTCCTGCTCAGCATTGACTCCGACAAGACCGACGAGGCGGAGCTCCAGCTCGCCGTCGACAACGCCACCAAGCTCGTCCTGGAGAAGCTCAACGCCAGCGTGGTGGAGTACACCAGCTTCGCCGACACCAAGACCATCCCGGGCCACTACGTCATCTACTGGGAGCTTCTAGTGAAGGACTCGGCCGAGTCGCCCAGCTCGGAATCGCTGAGTCAGTGCTGCTTGGCCATGGAGGAATCGCTCAATTCGGTTTACCGGCAGTGCCGGGTGGCGGACAACTCGATCGGGCCGTTGGAGATACGTGTGGTGAAGAGCGGCACGTTCGAGGAGTTGATGGACTACGCGATCTCGAGAGGCGCGTCGATTAACCAGTATAAGGTGCCGCGGTGTGTGAATTTTACCCCGATAATGGAGTTACTGGACTCCAGGGTGGTTTCCAAGCATTTTAGCCCTTCTTTACCGCATTGGACCCCGGAAAGGAGACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GH3

Protein Analysis

496

Amino Acids

56.31

Weight (kDa)

5.57

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH3 PF03321 3 - 231 5.7e-84 GH3 family N-terminal domain
GH3_M PF23571 258 - 332 1.5e-30 GH3 family central domain
GH3_C PF23572 348 - 467 4.1e-40 GH3 family C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013876)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14960
fragaria_vesca FvH4_2g04750
malus_domestica MD05G1092300.v1.1 MD05G1092900.v1.1
prunus_persica Prupe.8G137900_v2.0.a1
pyrus_communis pycom05g09190 pycom05g09210
rosa_chinensis RchiOBHm_Chr6g0254401
rosa_laevigata RLG00000014915
rosa_multiflora Rmu_ssc0000481.1_g000001
rosa_roxburghii Rroxscaffold_7G00210830
rosa_rugosa Rorug05G0552100
rosa_samantha Rh6AG069100 Rh6BG061700 Rh6CG061900 Rh6DG058900
rosa_wichuraiana Rw6G006070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 872
AccB1I GGYRCC 3 cut(s) 380, 970, 1381
AccBSI CCGCTC 2 cut(s) 514, 1317
AccI GTMKAC 3 cut(s) 276, 864, 1059
AccII CGCG 5 cut(s) 408, 930, 1346, 1361, 1387
AclWI GGATC 2 cut(s) 84, 257
AcoI YGGCCR 2 cut(s) 1185, 1227
AcsI RAATTY 3 cut(s) 6, 597, 1396
AcuI CTGAAG 2 cut(s) 15, 744
AcyI GRCGYC 2 cut(s) 381, 971
AdeI CACNNNGTG 1 cut(s) 988
AfaI GTAC 6 cut(s) 28, 158, 329, 610, 707, 1112
AfiI CCNNNNNNNGG 7 cut(s) 292, 600, 967, 1273, 1378, 1411, 1479
AflIII ACRYGT 1 cut(s) 1303
AgsI TTSAA 3 cut(s) 247, 320, 857
AjiI CACGTC 1 cut(s) 868
AjnI CCWGG 3 cut(s) 130, 1080, 1427
Alw21I GWGCWC 2 cut(s) 894, 1047
Alw26I GTCTC 3 cut(s) 404, 812, 1477
AlwI GGATC 2 cut(s) 84, 257
Ama87I CYCGRG 3 cut(s) 353, 1141, 1351
ApeKI GCWGC 2 cut(s) 436, 1221
ApoI RAATTY 3 cut(s) 6, 597, 1396
AspLEI GCGC 5 cut(s) 360, 383, 410, 973, 1361
AspS9I GGNCC 6 cut(s) 205, 603, 646, 1144, 1289, 1470
AsuC2I CCSGG 8 cut(s) 192, 491, 947, 955, 1142, 1143, 1268, 1475
AsuHPI GGTGA 4 cut(s) 50, 625, 943, 1321
AvaI CYCGRG 3 cut(s) 353, 1141, 1351
AvaII GGWCC 1 cut(s) 1470
BalI TGGCCA 1 cut(s) 1229
BanI GGYRCC 3 cut(s) 380, 970, 1381
BanII GRGCYC 2 cut(s) 894, 1047
BarI GAAGNNNNNNTAC 2 cut(s) 1103, 1135
Bbv12I GWGCWC 2 cut(s) 894, 1047
BbvI GCAGC 2 cut(s) 448, 1208
BccI CCATC 4 cut(s) 305, 419, 1145, 1330
BceAI ACGGC 3 cut(s) 368, 1040, 1276
BciT130I CCWGG 3 cut(s) 132, 1082, 1429
BcnI CCSGG 8 cut(s) 192, 491, 947, 955, 1142, 1143, 1268, 1475
BcoDI GTCTC 3 cut(s) 404, 812, 1477
BfaI CTAG 1 cut(s) 1172
BfmI CTRYAG 1 cut(s) 673
BfoI RGCGCY 3 cut(s) 361, 384, 974
BlpI GCTNAGC 1 cut(s) 1010
Bme18I GGWCC 1 cut(s) 1470
BmeT110I CYCGRG 3 cut(s) 353, 1141, 1351
BmgBI CACGTC 1 cut(s) 868
BmgT120I GGNCC 6 cut(s) 205, 603, 646, 1144, 1289, 1470
BmiI GGNNCC 5 cut(s) 382, 959, 972, 1383, 1472
BmrI ACTGGG 3 cut(s) 600, 643, 1171
BmsI GCATC 1 cut(s) 42
BmuI ACTGGG 3 cut(s) 600, 643, 1171
BplI GAGNNNNNCTC 2 cut(s) 809, 841
BpmI CTGGAG 4 cut(s) 407, 1031, 1103, 1411
Bpu1102I GCTNAGC 1 cut(s) 1010
BpuMI CCSGG 8 cut(s) 192, 491, 947, 955, 1142, 1143, 1268, 1475
BsaAI YACGTR 1 cut(s) 1304
BsaHI GRCGYC 2 cut(s) 381, 971
BsaI GGTCTC 2 cut(s) 404, 812
BsaJI CCNNGG 7 cut(s) 376, 690, 1141, 1230, 1385, 1428, 1473
BsaWI WCCGGW 3 cut(s) 395, 461, 635
Bsc4I CCNNNNNNNGG 7 cut(s) 292, 600, 967, 1273, 1378, 1411, 1479
Bse118I RCCGGY 1 cut(s) 1257
Bse1I ACTGG 8 cut(s) 110, 437, 606, 649, 864, 1166, 1372, 1425
BseBI CCWGG 3 cut(s) 132, 1082, 1429
BseDI CCNNGG 7 cut(s) 376, 690, 1141, 1230, 1385, 1428, 1473
BseGI GGATG 4 cut(s) 297, 411, 453, 1137
BseLI CCNNNNNNNGG 7 cut(s) 292, 600, 967, 1273, 1378, 1411, 1479
BseMII CTCAG 4 cut(s) 534, 840, 1024, 1202
BseNI ACTGG 8 cut(s) 110, 437, 606, 649, 864, 1166, 1372, 1425
BseRI GAGGAG 6 cut(s) 83, 92, 341, 392, 584, 1343
BseX3I CGGCCG 1 cut(s) 1185
BseXI GCAGC 2 cut(s) 448, 1208
BseYI CCCAGC 1 cut(s) 1196
BsgI GTGCAG 2 cut(s) 682, 772
Bsh1236I CGCG 5 cut(s) 408, 930, 1346, 1361, 1387
Bsh1285I CGRYCG 2 cut(s) 1188, 1287
BshNI GGYRCC 3 cut(s) 380, 970, 1381
BsiEI CGRYCG 2 cut(s) 1188, 1287
BsiHKAI GWGCWC 2 cut(s) 894, 1047
BsiHKCI CYCGRG 3 cut(s) 353, 1141, 1351
BslI CCNNNNNNNGG 7 cut(s) 292, 600, 967, 1273, 1378, 1411, 1479
BsmAI GTCTC 3 cut(s) 404, 812, 1477
BsmBI CGTCTC 1 cut(s) 1477
BsmI GAATGC 1 cut(s) 509
Bso31I GGTCTC 2 cut(s) 404, 812
BsoBI CYCGRG 3 cut(s) 353, 1141, 1351
Bsp1286I GDGCHC 2 cut(s) 894, 1047
Bsp1407I TGTACA 1 cut(s) 327
Bsp143I GATC 4 cut(s) 76, 262, 1284, 1347
Bsp1720I GCTNAGC 1 cut(s) 1010
Bsp19I CCATGG 2 cut(s) 690, 1230
BspCNI CTCAG 4 cut(s) 535, 839, 1023, 1203
BspFNI CGCG 5 cut(s) 408, 930, 1346, 1361, 1387
BspHI TCATGA 1 cut(s) 114
BspLI GGNNCC 5 cut(s) 382, 959, 972, 1383, 1472
BspPI GGATC 2 cut(s) 84, 257
BspQI GCTCTTC 1 cut(s) 1307
BspT107I GGYRCC 3 cut(s) 380, 970, 1381
BspTNI GGTCTC 2 cut(s) 404, 812
BsrBI CCGCTC 2 cut(s) 514, 1317
BsrFI RCCGGY 1 cut(s) 1257
BsrGI TGTACA 1 cut(s) 327
BsrI ACTGG 8 cut(s) 110, 437, 606, 649, 864, 1166, 1372, 1425
BssAI RCCGGY 1 cut(s) 1257
BssECI CCNNGG 7 cut(s) 376, 690, 1141, 1230, 1385, 1428, 1473
BssMI GATC 4 cut(s) 76, 262, 1284, 1347
BssNI GRCGYC 2 cut(s) 381, 971
BssT1I CCWWGG 2 cut(s) 690, 1230
Bst2UI CCWGG 3 cut(s) 132, 1082, 1429
Bst4CI ACNGT 1 cut(s) 1488
Bst6I CTCTTC 1 cut(s) 1307
BstACI GRCGYC 2 cut(s) 381, 971
BstAUI TGTACA 1 cut(s) 327
BstBAI YACGTR 1 cut(s) 1304
BstC8I GCNNGC 4 cut(s) 239, 697, 1053, 1099
BstDEI CTNAG 4 cut(s) 543, 826, 1010, 1211
BstDSI CCRYGG 3 cut(s) 690, 1230, 1385
BstEII GGTNACC 1 cut(s) 949
BstF5I GGATG 4 cut(s) 297, 411, 453, 1137
BstFNI CGCG 5 cut(s) 408, 930, 1346, 1361, 1387
BstH2I RGCGCY 3 cut(s) 361, 384, 974
BstHHI GCGC 5 cut(s) 360, 383, 410, 973, 1361
BstKTI GATC 4 cut(s) 79, 265, 1287, 1350
BstMAI GTCTC 3 cut(s) 404, 812, 1477
BstMBI GATC 4 cut(s) 76, 262, 1284, 1347
BstMCI CGRYCG 2 cut(s) 1188, 1287
BstMWI GCNNNNNNNGC 2 cut(s) 389, 433
BstNI CCWGG 3 cut(s) 132, 1082, 1429
BstPI GGTNACC 1 cut(s) 949
BstSFI CTRYAG 1 cut(s) 673
BstUI CGCG 5 cut(s) 408, 930, 1346, 1361, 1387
BstV1I GCAGC 2 cut(s) 448, 1208
BstXI CCANNNNNNTGG 1 cut(s) 1105
BstZI CGGCCG 1 cut(s) 1185
BtgI CCRYGG 3 cut(s) 690, 1230, 1385
BtrI CACGTC 1 cut(s) 868
BtsCI GGATG 4 cut(s) 297, 411, 453, 1137
BtsI GCAGTG 1 cut(s) 1268
BtsIMutI CAGTG 3 cut(s) 834, 1223, 1268
Cac8I GCNNGC 4 cut(s) 239, 697, 1053, 1099
CciI TCATGA 1 cut(s) 114
CfoI GCGC 5 cut(s) 360, 383, 410, 973, 1361
Cfr10I RCCGGY 1 cut(s) 1257
Cfr13I GGNCC 6 cut(s) 205, 603, 646, 1144, 1289, 1470
Cfr42I CCGCGG 1 cut(s) 1388
Cfr9I CCCGGG 1 cut(s) 1141
CseI GACGC 2 cut(s) 919, 1350
Csp6I GTAC 6 cut(s) 27, 157, 328, 609, 706, 1111
CviAII CATG 9 cut(s) 106, 115, 325, 508, 691, 703, 748, 790, 1231
CviQI GTAC 6 cut(s) 27, 157, 328, 609, 706, 1111
DdeI CTNAG 4 cut(s) 543, 826, 1010, 1211
DinI GGCGCC 2 cut(s) 382, 972
DpnI GATC 4 cut(s) 78, 264, 1286, 1349
DpnII GATC 4 cut(s) 76, 262, 1284, 1347
DraIII CACNNNGTG 1 cut(s) 988
DrdI GACNNNNNNGTC 1 cut(s) 872
DseDI GACNNNNNNGTC 1 cut(s) 872
EaeI YGGCCR 2 cut(s) 1185, 1227
EagI CGGCCG 1 cut(s) 1185
Eam1104I CTCTTC 1 cut(s) 1307
EarI CTCTTC 1 cut(s) 1307
EciI GGCGGA 5 cut(s) 210, 362, 1009, 1055, 1289
Ecl136II GAGCTC 2 cut(s) 892, 1045
EclXI CGGCCG 1 cut(s) 1185
Eco130I CCWWGG 2 cut(s) 690, 1230
Eco24I GRGCYC 2 cut(s) 894, 1047
Eco31I GGTCTC 2 cut(s) 404, 812
Eco47I GGWCC 1 cut(s) 1470
Eco52I CGGCCG 1 cut(s) 1185
Eco53kI GAGCTC 2 cut(s) 892, 1045
Eco57I CTGAAG 2 cut(s) 15, 744
Eco88I CYCGRG 3 cut(s) 353, 1141, 1351
Eco91I GGTNACC 1 cut(s) 949
EcoICRI GAGCTC 2 cut(s) 892, 1045
EcoO65I GGTNACC 1 cut(s) 949
EcoRII CCWGG 3 cut(s) 130, 1080, 1427
EcoT14I CCWWGG 2 cut(s) 690, 1230
EcoT22I ATGCAT 1 cut(s) 509
EcoT38I GRGCYC 2 cut(s) 894, 1047
EgeI GGCGCC 2 cut(s) 382, 972
EheI GGCGCC 2 cut(s) 382, 972
ErhI CCWWGG 2 cut(s) 690, 1230
Esp3I CGTCTC 1 cut(s) 1477
FaeI CATG 9 cut(s) 109, 118, 328, 511, 694, 706, 751, 793, 1234
FatI CATG 9 cut(s) 105, 114, 324, 507, 690, 702, 747, 789, 1230
FauI CCCGC 1 cut(s) 28
FblI GTMKAC 3 cut(s) 276, 864, 1059
FokI GGATG 4 cut(s) 284, 398, 440, 1124
FriOI GRGCYC 2 cut(s) 894, 1047
FspBI CTAG 1 cut(s) 1172
GlaI GCGC 5 cut(s) 359, 382, 409, 972, 1360
GsaI CCCAGC 1 cut(s) 1200
GsuI CTGGAG 4 cut(s) 407, 1031, 1103, 1411
HaeII RGCGCY 3 cut(s) 361, 384, 974
HgaI GACGC 2 cut(s) 919, 1350
HhaI GCGC 5 cut(s) 360, 383, 410, 973, 1361
Hin1I GRCGYC 2 cut(s) 381, 971
Hin1II CATG 9 cut(s) 109, 118, 328, 511, 694, 706, 751, 793, 1234
Hin6I GCGC 5 cut(s) 358, 381, 408, 971, 1359
HinP1I GCGC 5 cut(s) 358, 381, 408, 971, 1359
HincII GTYRAC 2 cut(s) 865, 1060
HindII GTYRAC 2 cut(s) 865, 1060
HphI GGTGA 4 cut(s) 50, 625, 943, 1321
Hpy166II GTNNAC 6 cut(s) 277, 330, 865, 1060, 1113, 1255
Hpy188I TCNGA 8 cut(s) 178, 660, 718, 731, 763, 878, 1024, 1204
Hpy188III TCNNGA 7 cut(s) 65, 115, 247, 620, 836, 1351, 1353
Hpy8I GTNNAC 6 cut(s) 277, 330, 865, 1060, 1113, 1255
Hpy99I CGWCG 6 cut(s) 84, 869, 935, 1037, 1061, 1366
HpyAV CCTTC 3 cut(s) 768, 1171, 1464
HpyCH4III ACNGT 1 cut(s) 1488
HpyCH4IV ACGT 5 cut(s) 867, 1002, 1152, 1303, 1322
HpyCH4V TGCA 3 cut(s) 507, 699, 753
HpyF10VI GCNNNNNNNGC 2 cut(s) 389, 433
HpyF3I CTNAG 4 cut(s) 543, 826, 1010, 1211
HpySE526I ACGT 5 cut(s) 867, 1002, 1152, 1303, 1322
Hsp92I GRCGYC 2 cut(s) 381, 971
Hsp92II CATG 9 cut(s) 109, 118, 328, 511, 694, 706, 751, 793, 1234
HspAI GCGC 5 cut(s) 358, 381, 408, 971, 1359
KasI GGCGCC 2 cut(s) 380, 970
KspI CCGCGG 1 cut(s) 1388
Kzo9I GATC 4 cut(s) 76, 262, 1284, 1347
LguI GCTCTTC 1 cut(s) 1307
LmnI GCTCC 5 cut(s) 511, 638, 1042, 1050, 1165
Lsp1109I GCAGC 2 cut(s) 448, 1208
LweI GCATC 1 cut(s) 42
MaeI CTAG 1 cut(s) 1172
MaeII ACGT 5 cut(s) 867, 1002, 1152, 1303, 1322
MaeIII GTNAC 5 cut(s) 14, 631, 722, 949, 1416
MalI GATC 4 cut(s) 78, 264, 1286, 1349
MbiI CCGCTC 2 cut(s) 514, 1317
MboI GATC 4 cut(s) 76, 262, 1284, 1347
MboII GAAGA 2 cut(s) 1009, 1324
MhlI GDGCHC 2 cut(s) 894, 1047
MlsI TGGCCA 1 cut(s) 1229
MluCI AATT 7 cut(s) 6, 480, 574, 597, 777, 1246, 1396
MluNI TGGCCA 1 cut(s) 1229
Mly113I GGCGCC 2 cut(s) 381, 971
MmeI TCCRAC 5 cut(s) 54, 683, 856, 1047, 1275
Mox20I TGGCCA 1 cut(s) 1229
Mph1103I ATGCAT 1 cut(s) 509
MscI TGGCCA 1 cut(s) 1229
MseI TTAA 1 cut(s) 1368
MslI CAYNNNNRTG 2 cut(s) 335, 779
Msp20I TGGCCA 1 cut(s) 1229
MspA1I CMGCKG 3 cut(s) 35, 678, 1387
Mva1269I GAATGC 1 cut(s) 509
MvaI CCWGG 3 cut(s) 132, 1082, 1429
MvnI CGCG 5 cut(s) 408, 930, 1346, 1361, 1387
MwoI GCNNNNNNNGC 2 cut(s) 389, 433
NarI GGCGCC 2 cut(s) 381, 971
NciI CCSGG 8 cut(s) 192, 491, 947, 955, 1142, 1143, 1268, 1475
NcoI CCATGG 2 cut(s) 690, 1230
NdeII GATC 4 cut(s) 76, 262, 1284, 1347
NlaIII CATG 9 cut(s) 109, 118, 328, 511, 694, 706, 751, 793, 1234
NlaIV GGNNCC 5 cut(s) 382, 959, 972, 1383, 1472
NmeAIII GCCGAG 4 cut(s) 357, 947, 1163, 1213
NmuCI GTSAC 2 cut(s) 631, 949
NsiI ATGCAT 1 cut(s) 509
PaeR7I CTCGAG 2 cut(s) 353, 1351
PagI TCATGA 1 cut(s) 114
PciSI GCTCTTC 1 cut(s) 1307
PcsI WCGNNNNNNNCGW 5 cut(s) 174, 627, 714, 1289, 1358
PctI GAATGC 1 cut(s) 509
PfeI GAWTC 5 cut(s) 61, 179, 585, 1205, 1238
PfoI TCCNGGA 1 cut(s) 1080
Ple19I CGATCG 1 cut(s) 1287
PluTI GGCGCC 2 cut(s) 384, 974
Ppu21I YACGTR 1 cut(s) 1304
Psp124BI GAGCTC 2 cut(s) 894, 1047
Psp6I CCWGG 3 cut(s) 130, 1080, 1427
PspEI GGTNACC 1 cut(s) 949
PspFI CCCAGC 1 cut(s) 1196
PspGI CCWGG 3 cut(s) 130, 1080, 1427
PspN4I GGNNCC 5 cut(s) 382, 959, 972, 1383, 1472
PspPI GGNCC 6 cut(s) 205, 603, 646, 1144, 1289, 1470
PspXI VCTCGAGB 1 cut(s) 353
PsrI GAACNNNNNNTAC 2 cut(s) 149, 181
PvuI CGATCG 1 cut(s) 1287
RsaI GTAC 6 cut(s) 28, 158, 329, 610, 707, 1112
RsaNI GTAC 6 cut(s) 27, 157, 328, 609, 706, 1111
RseI CAYNNNNRTG 2 cut(s) 335, 779
SacI GAGCTC 2 cut(s) 894, 1047
SacII CCGCGG 1 cut(s) 1388
SalI GTCGAC 2 cut(s) 863, 1058
SapI GCTCTTC 1 cut(s) 1307
SaqAI TTAA 1 cut(s) 1368
Sau3AI GATC 4 cut(s) 76, 262, 1284, 1347
Sau96I GGNCC 6 cut(s) 205, 603, 646, 1144, 1289, 1470
SduI GDGCHC 2 cut(s) 894, 1047
SfaNI GCATC 1 cut(s) 42
SfcI CTRYAG 1 cut(s) 673
SfoI GGCGCC 2 cut(s) 382, 972
Sfr274I CTCGAG 2 cut(s) 353, 1351
Sfr303I CCGCGG 1 cut(s) 1388
SgrBI CCGCGG 1 cut(s) 1388
SinI GGWCC 1 cut(s) 1470
SlaI CTCGAG 2 cut(s) 353, 1351
SmaI CCCGGG 1 cut(s) 1143
SmiMI CAYNNNNRTG 2 cut(s) 335, 779
SmlI CTYRAG 2 cut(s) 353, 1351
SmoI CTYRAG 2 cut(s) 353, 1351
Sse9I AATT 7 cut(s) 6, 480, 574, 597, 777, 1246, 1396
SspDI GGCGCC 2 cut(s) 380, 970
SspMI CTAG 1 cut(s) 1172
SstI GAGCTC 2 cut(s) 894, 1047
StyI CCWWGG 2 cut(s) 690, 1230
TaaI ACNGT 1 cut(s) 1488
TaiI ACGT 5 cut(s) 870, 1005, 1155, 1306, 1325
TaqI TCGA 9 cut(s) 354, 621, 801, 864, 1059, 1283, 1326, 1352, 1364
TaqII GACCGA 1 cut(s) 1046
TasI AATT 7 cut(s) 6, 480, 574, 597, 777, 1246, 1396
TatI WGTACW 4 cut(s) 156, 327, 608, 1110
TauI GCSGC 8 cut(s) 449, 567, 681, 684, 976, 994, 1320, 1387
TfiI GAWTC 5 cut(s) 61, 179, 585, 1205, 1238
Tru1I TTAA 1 cut(s) 1368
Tru9I TTAA 1 cut(s) 1368
TscAI CASTG 3 cut(s) 834, 1223, 1268
TseFI GTSAC 2 cut(s) 631, 949
TseI GCWGC 2 cut(s) 436, 1221
Tsp45I GTSAC 2 cut(s) 631, 949
TspDTI ATGAA 2 cut(s) 131, 759
TspMI CCCGGG 1 cut(s) 1141
TspRI CASTG 3 cut(s) 834, 1223, 1268
VpaK11BI GGWCC 1 cut(s) 1470
XapI RAATTY 3 cut(s) 6, 597, 1396
XcmI CCANNNNNNNNNTGG 1 cut(s) 1078
XhoI CTCGAG 2 cut(s) 353, 1351
XmaI CCCGGG 1 cut(s) 1141
XmiI GTMKAC 3 cut(s) 276, 864, 1059
XspI CTAG 1 cut(s) 1172
Zsp2I ATGCAT 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.