MD05G1092300.v1.1

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013876
Physical Location & Seq
Forward (+)
19618797 .. 19621192
2396 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1092300.v1.1.491

Sequence Viewer

Length: 1734 bp
ATGGCTGTCGACTCGTCACTCTCTCCCCTCGGCCCACCCGCCTGCGAGAAGGACGCCAAAGCGTTACAGTTCATTGAAGAAACTACCCGGAACGCCAACGCTGTCCAAGAGCGGGTGCTGTCCGAGATACTAGCTCGAAACGCCCAGACCGAGTACCTTAAACGCTTCAAACTCGGCGGCGCCACCGACCGCAAAACCTTCAAGTCCAAACTCCCCGTGATTACCTACGAGGATCTCCAGCCCGAAATCCAACGCATTGCCAATGGTGATCGCTCCCCCATCTTGTCAGCTCATCCCATCTCAGAATTCCTCACCAGTTCTGGGACTTCAGCTGGAGAGAGGAAGCTGATGCCAACAATTCAGGAGGAATTGGATCGCCGCCAATTATTATACAGCCTTCTCATGCCCGTCATGAACCTTTGTGTGCCCGGGCTGGACAAGGGAAAGGGCCTATACTTTCTGTTCGTGAAGTCCGAAACAAAGACGCCGGGCGGCCTTTTGGCCCGACCCGTTCTCACCAGCTACTACAAAAGCGAGCACTTCAAGACCATGTACGCCCAAATGCTCTGCGGCCTCCTCGAGCGCGAAAAAGTCCTCCGCCTCGGCGCCGTTTTTGCGTCCGGTCTCCTCCGTGCAATACGGTTCCTCCAGCTCAACTGGCAACAACTAGCAGAAGACATCCGGACCGGAACTCTGAGCTCGAAAATAACTGACCCGGATTTGAAATCTTGCATAGGCGGGATCTTAAAACCCCACCCGGAGCTAGCGGATTTCATGTCCAAACAGTGCGGAAACGAAAATTGGGATGGGATTTTAACTCGGATATGGCCAAACACGAAATATCTGGATGTGATAGTGACTGGCGCGATGGCGCAGTACATTCCGACGCTGGATTACTACAGCGGCGGATTGCCGATGGCATGTACGATGTACGCATCGTCGGAGTGTTATTTCGGACTCAATCTCAACCCTATGTGCAAGCCTTCTGAAGTTTCCTACACGATAATGCCAAACATGGCCTATTTCGAGTTCCTCCCTTACGAGCCAAACTTGGTGAGTGGCGGCGGCGAGTCGGGAACCAGACTCGTTGACCTCGTCGACGTCGAGGTCGGAAGAGAGTACGAGCTCGTAATCACCACCTACGCCGGGCTTCACAGATACAGAGTCGGGGACATCCTCCGAGTCACCGGGTTCCACAACTCGGCCCCGCAGTTCCACTTCGTGAGGAGGAAGAACGTTTTGCTCAGCATCGACTCGGACAAGACCGACGAAGCAGAGTTGCAACTCGCCGTCGACAACGCCTCCAAGCTCCTCAAGAAATTCAGCGCTAGCGTGGTGGAGTACACGAGTTACGCCGACACGACGACAATCCCGGGGCACTACGTGATCTATTGGGAGCTTCTCGTGAAGGACTCGGCGAACTCAGAGAGTGAGTCGTTCGACGCGGTGATGGACCAGTGCTGCCTAACGATGGAGGAATCACTTAACTCGGTGTATCGGCAGGGTCGAGTGGCGGACAACTCGATCGGGCCGCTAGAGATACGCGTGGTGAAGAACGGCACGTTTGAGGAGTTGATGGACTACGCGATCTCGAGAGGCGCGTCGATTAATCAGTACAAGGTACCGCGGTGTGTGAATTTTACCCCAATCATGGAGTTACTGGACTTCAGGGTAATTTCCAAGCATGTCAGCCCATCTTTACCGCACTGGACTCCTGAAAGGAAGATGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GH3

Protein Analysis

578

Amino Acids

64.77

Weight (kDa)

6.08

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH3 PF03321 22 - 306 2.4e-106 GH3 family N-terminal domain
GH3_M PF23571 333 - 410 5.3e-29 GH3 family central domain
GH3_C PF23572 426 - 547 4.6e-40 GH3 family C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013876)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14960
fragaria_vesca FvH4_2g04750
malus_domestica MD05G1092300.v1.1 MD05G1092900.v1.1
prunus_persica Prupe.8G137900_v2.0.a1
pyrus_communis pycom05g09190 pycom05g09210
rosa_chinensis RchiOBHm_Chr6g0254401
rosa_laevigata RLG00000014915
rosa_multiflora Rmu_ssc0000481.1_g000001
rosa_roxburghii Rroxscaffold_7G00210830
rosa_rugosa Rorug05G0552100
rosa_samantha Rh6AG069100 Rh6BG061700 Rh6CG061900 Rh6DG058900
rosa_wichuraiana Rw6G006070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1106
AatII GACGTC 1 cut(s) 1104
Acc65I GGTACC 1 cut(s) 1621
AccB1I GGYRCC 3 cut(s) 179, 605, 1621
AccBSI CCGCTC 1 cut(s) 112
AccI GTMKAC 3 cut(s) 9, 1098, 1293
AccII CGCG 7 cut(s) 585, 866, 1445, 1545, 1586, 1601, 1627
AccIII TCCGGA 1 cut(s) 681
AclI AACGTT 1 cut(s) 1236
AclWI GGATC 3 cut(s) 240, 381, 749
AcoI YGGCCR 1 cut(s) 827
AcsI RAATTY 3 cut(s) 305, 1319, 1636
AcuI CTGAAG 3 cut(s) 312, 1008, 1651
AcyI GRCGYC 5 cut(s) 54, 180, 485, 606, 1101
AdeI CACNNNGTG 2 cut(s) 1222, 1384
AfaI GTAC 9 cut(s) 155, 554, 878, 925, 932, 1121, 1343, 1616, 1623
AfeI AGCGCT 1 cut(s) 1327
AfiI CCNNNNNNNGG 6 cut(s) 112, 321, 1146, 1201, 1471, 1651
AflIII ACRYGT 1 cut(s) 1543
AgsI TTSAA 5 cut(s) 77, 169, 202, 544, 724
AjuI GAANNNNNNNTTGG 2 cut(s) 784, 816
Alw21I GWGCWC 3 cut(s) 540, 701, 1128
Alw26I GTCTC 1 cut(s) 629
AlwI GGATC 3 cut(s) 240, 381, 749
Ama87I CYCGRG 4 cut(s) 428, 578, 1372, 1591
Aor13HI TCCGGA 1 cut(s) 681
Aor51HI AGCGCT 1 cut(s) 1327
AoxI GGCC 9 cut(s) 31, 448, 493, 501, 571, 827, 1017, 1203, 1529
ApeKI GCWGC 1 cut(s) 1461
ApoI RAATTY 3 cut(s) 305, 1319, 1636
AseI ATTAAT 1 cut(s) 1608
Asp718I GGTACC 1 cut(s) 1621
AspLEI GCGC 7 cut(s) 182, 585, 608, 866, 874, 1328, 1601
AspS9I GGNCC 7 cut(s) 32, 448, 502, 684, 1204, 1453, 1529
AsuHPI GGTGA 8 cut(s) 278, 304, 508, 1066, 1126, 1177, 1459, 1561
AsuNHI GCTAGC 2 cut(s) 763, 1328
AvaI CYCGRG 4 cut(s) 428, 578, 1372, 1591
AvaII GGWCC 2 cut(s) 684, 1453
BaeGI GKGCMC 2 cut(s) 429, 1380
BaeI ACNNNNGTAYC 2 cut(s) 1613, 1646
BalI TGGCCA 1 cut(s) 829
BanI GGYRCC 3 cut(s) 179, 605, 1621
BanII GRGCYC 2 cut(s) 701, 1128
BauI CACGAG 2 cut(s) 1345, 1403
BbsI GAAGAC 1 cut(s) 681
Bbv12I GWGCWC 3 cut(s) 540, 701, 1128
BbvI GCAGC 1 cut(s) 1448
BccI CCATC 9 cut(s) 287, 305, 800, 862, 910, 1444, 1465, 1570, 1702
BceAI ACGGC 3 cut(s) 593, 1274, 1573
BcoDI GTCTC 1 cut(s) 629
BfaI CTAG 5 cut(s) 131, 668, 764, 1329, 1535
BfmI CTRYAG 1 cut(s) 898
BfoI RGCGCY 3 cut(s) 183, 609, 1329
BisI GCNGC 9 cut(s) 178, 379, 493, 571, 904, 1063, 1066, 1462, 1532
BlpI GCTNAGC 1 cut(s) 1244
BlsI GCNGC 9 cut(s) 179, 380, 494, 572, 905, 1064, 1067, 1463, 1533
Bme18I GGWCC 2 cut(s) 684, 1453
BmeT110I CYCGRG 4 cut(s) 428, 578, 1372, 1591
BmgT120I GGNCC 7 cut(s) 32, 448, 502, 684, 1204, 1453, 1529
BmiI GGNNCC 7 cut(s) 181, 607, 644, 1078, 1193, 1206, 1623
BmsI GCATC 4 cut(s) 339, 944, 1257, 1716
BmtI GCTAGC 2 cut(s) 767, 1332
BpiI GAAGAC 1 cut(s) 681
BpmI CTGGAG 3 cut(s) 221, 354, 632
Bpu1102I GCTNAGC 1 cut(s) 1244
BpuEI CTTGAG 1 cut(s) 1298
BsaAI YACGTR 1 cut(s) 1384
BsaHI GRCGYC 5 cut(s) 54, 180, 485, 606, 1101
BsaI GGTCTC 1 cut(s) 629
BsaJI CCNNGG 6 cut(s) 28, 428, 601, 1372, 1373, 1625
BsaWI WCCGGW 3 cut(s) 620, 681, 686
BsaXI ACNNNNNCTCC 4 cut(s) 630, 660, 1286, 1316
Bsc4I CCNNNNNNNGG 6 cut(s) 112, 321, 1146, 1201, 1471, 1651
Bse1I ACTGG 6 cut(s) 315, 662, 865, 1456, 1665, 1712
Bse3DI GCAATG 1 cut(s) 255
BseAI TCCGGA 1 cut(s) 681
BseDI CCNNGG 6 cut(s) 28, 428, 601, 1372, 1373, 1625
BseGI GGATG 5 cut(s) 292, 678, 811, 853, 1173
BseLI CCNNNNNNNGG 6 cut(s) 112, 321, 1146, 1201, 1471, 1651
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 4 cut(s) 315, 686, 1258, 1437
BseNI ACTGG 6 cut(s) 315, 662, 865, 1456, 1665, 1712
BseRI GAGGAG 5 cut(s) 566, 617, 1240, 1301, 1583
BseSI GKGCMC 2 cut(s) 429, 1380
BseXI GCAGC 1 cut(s) 1448
Bsh1236I CGCG 7 cut(s) 585, 866, 1445, 1545, 1586, 1601, 1627
Bsh1285I CGRYCG 2 cut(s) 190, 1527
BshFI GGCC 9 cut(s) 33, 450, 495, 503, 573, 829, 1019, 1205, 1531
BshNI GGYRCC 3 cut(s) 179, 605, 1621
BsiEI CGRYCG 2 cut(s) 190, 1527
BsiHKAI GWGCWC 3 cut(s) 540, 701, 1128
BsiHKCI CYCGRG 4 cut(s) 428, 578, 1372, 1591
BslFI GGGAC 2 cut(s) 337, 1184
BslI CCNNNNNNNGG 6 cut(s) 112, 321, 1146, 1201, 1471, 1651
BsmAI GTCTC 1 cut(s) 629
BsmFI GGGAC 2 cut(s) 337, 1184
BsnI GGCC 9 cut(s) 33, 450, 495, 503, 573, 829, 1019, 1205, 1531
Bso31I GGTCTC 1 cut(s) 629
BsoBI CYCGRG 4 cut(s) 428, 578, 1372, 1591
Bsp1286I GDGCHC 5 cut(s) 429, 540, 701, 1128, 1380
Bsp13I TCCGGA 1 cut(s) 681
Bsp143I GATC 7 cut(s) 232, 268, 373, 741, 1386, 1524, 1587
Bsp1720I GCTNAGC 1 cut(s) 1244
BspANI GGCC 9 cut(s) 33, 450, 495, 503, 573, 829, 1019, 1205, 1531
BspCNI CTCAG 4 cut(s) 314, 687, 1257, 1436
BspEI TCCGGA 1 cut(s) 681
BspFNI CGCG 7 cut(s) 585, 866, 1445, 1545, 1586, 1601, 1627
BspHI TCATGA 1 cut(s) 411
BspLI GGNNCC 7 cut(s) 181, 607, 644, 1078, 1193, 1206, 1623
BspOI GCTAGC 2 cut(s) 767, 1332
BspPI GGATC 3 cut(s) 240, 381, 749
BspT107I GGYRCC 3 cut(s) 179, 605, 1621
BspTNI GGTCTC 1 cut(s) 629
BsrBI CCGCTC 1 cut(s) 112
BsrDI GCAATG 1 cut(s) 255
BsrI ACTGG 6 cut(s) 315, 662, 865, 1456, 1665, 1712
BssECI CCNNGG 6 cut(s) 28, 428, 601, 1372, 1373, 1625
BssMI GATC 7 cut(s) 232, 268, 373, 741, 1386, 1524, 1587
BssNI GRCGYC 5 cut(s) 54, 180, 485, 606, 1101
BssSI CACGAG 2 cut(s) 1345, 1403
Bst2BI CACGAG 2 cut(s) 1345, 1403
Bst4CI ACNGT 3 cut(s) 69, 642, 786
Bst6I CTCTTC 1 cut(s) 1108
BstACI GRCGYC 5 cut(s) 54, 180, 485, 606, 1101
BstBAI YACGTR 1 cut(s) 1384
BstC8I GCNNGC 5 cut(s) 43, 536, 765, 980, 1330
BstDEI CTNAG 4 cut(s) 301, 695, 1244, 1423
BstDSI CCRYGG 1 cut(s) 1625
BstF5I GGATG 5 cut(s) 292, 678, 811, 853, 1173
BstFNI CGCG 7 cut(s) 585, 866, 1445, 1545, 1586, 1601, 1627
BstH2I RGCGCY 3 cut(s) 183, 609, 1329
BstHHI GCGC 7 cut(s) 182, 585, 608, 866, 874, 1328, 1601
BstKTI GATC 7 cut(s) 235, 271, 376, 744, 1389, 1527, 1590
BstMAI GTCTC 1 cut(s) 629
BstMBI GATC 7 cut(s) 232, 268, 373, 741, 1386, 1524, 1587
BstMCI CGRYCG 2 cut(s) 190, 1527
BstMWI GCNNNNNNNGC 3 cut(s) 140, 614, 658
BstNSI RCATGY 2 cut(s) 924, 1688
BstSFI CTRYAG 1 cut(s) 898
BstSLI GKGCMC 2 cut(s) 429, 1380
BstUI CGCG 7 cut(s) 585, 866, 1445, 1545, 1586, 1601, 1627
BstV1I GCAGC 1 cut(s) 1448
BstV2I GAAGAC 1 cut(s) 681
BstX2I RGATCY 2 cut(s) 232, 741
BstYI RGATCY 2 cut(s) 232, 741
BsuRI GGCC 9 cut(s) 33, 450, 495, 503, 573, 829, 1019, 1205, 1531
BtgI CCRYGG 1 cut(s) 1625
BtgZI GCGATG 1 cut(s) 881
BtsCI GGATG 5 cut(s) 292, 678, 811, 853, 1173
BtsIMutI CAGTG 3 cut(s) 791, 1463, 1705
Cac8I GCNNGC 5 cut(s) 43, 536, 765, 980, 1330
CciI TCATGA 1 cut(s) 411
CfoI GCGC 7 cut(s) 182, 585, 608, 866, 874, 1328, 1601
Cfr13I GGNCC 7 cut(s) 32, 448, 502, 684, 1204, 1453, 1529
Cfr42I CCGCGG 1 cut(s) 1628
Cfr9I CCCGGG 2 cut(s) 428, 1372
CpoI CGGWCCG 1 cut(s) 684
CseI GACGC 6 cut(s) 62, 493, 606, 895, 1451, 1590
Csp6I GTAC 9 cut(s) 154, 553, 877, 924, 931, 1120, 1342, 1615, 1622
CspI CGGWCCG 1 cut(s) 684
CviAII CATG 8 cut(s) 403, 412, 550, 775, 921, 1015, 1651, 1685
CviQI GTAC 9 cut(s) 154, 553, 877, 924, 931, 1120, 1342, 1615, 1622
DdeI CTNAG 4 cut(s) 301, 695, 1244, 1423
DinI GGCGCC 2 cut(s) 181, 607
DpnI GATC 7 cut(s) 234, 270, 375, 743, 1388, 1526, 1589
DpnII GATC 7 cut(s) 232, 268, 373, 741, 1386, 1524, 1587
DraIII CACNNNGTG 2 cut(s) 1222, 1384
DrdI GACNNNNNNGTC 1 cut(s) 1106
DseDI GACNNNNNNGTC 1 cut(s) 1106
EaeI YGGCCR 1 cut(s) 827
Eam1104I CTCTTC 1 cut(s) 1108
EarI CTCTTC 1 cut(s) 1108
EciI GGCGGA 3 cut(s) 587, 921, 1529
Ecl136II GAGCTC 2 cut(s) 699, 1126
Eco24I GRGCYC 2 cut(s) 701, 1128
Eco31I GGTCTC 1 cut(s) 629
Eco47I GGWCC 2 cut(s) 684, 1453
Eco47III AGCGCT 1 cut(s) 1327
Eco53kI GAGCTC 2 cut(s) 699, 1126
Eco57I CTGAAG 3 cut(s) 312, 1008, 1651
Eco88I CYCGRG 4 cut(s) 428, 578, 1372, 1591
EcoICRI GAGCTC 2 cut(s) 699, 1126
EcoO109I RGGNCCY 1 cut(s) 448
EcoRI GAATTC 1 cut(s) 305
EcoT38I GRGCYC 2 cut(s) 701, 1128
EgeI GGCGCC 2 cut(s) 181, 607
EheI GGCGCC 2 cut(s) 181, 607
FaeI CATG 8 cut(s) 406, 415, 553, 778, 924, 1018, 1654, 1688
FaqI GGGAC 2 cut(s) 337, 1184
FatI CATG 8 cut(s) 402, 411, 549, 774, 920, 1014, 1650, 1684
FauI CCCGC 4 cut(s) 46, 105, 731, 1215
FblI GTMKAC 3 cut(s) 9, 1098, 1293
Fnu4HI GCNGC 9 cut(s) 178, 379, 493, 571, 904, 1063, 1066, 1462, 1532
FokI GGATG 5 cut(s) 279, 665, 818, 860, 1160
FriOI GRGCYC 2 cut(s) 701, 1128
Fsp4HI GCNGC 9 cut(s) 178, 379, 493, 571, 904, 1063, 1066, 1462, 1532
FspBI CTAG 5 cut(s) 131, 668, 764, 1329, 1535
GlaI GCGC 7 cut(s) 181, 584, 607, 865, 873, 1327, 1600
GluI GCNGC 9 cut(s) 178, 379, 493, 571, 904, 1063, 1066, 1462, 1532
GsuI CTGGAG 3 cut(s) 221, 354, 632
HaeII RGCGCY 3 cut(s) 183, 609, 1329
HaeIII GGCC 9 cut(s) 33, 450, 495, 503, 573, 829, 1019, 1205, 1531
HgaI GACGC 6 cut(s) 62, 493, 606, 895, 1451, 1590
HhaI GCGC 7 cut(s) 182, 585, 608, 866, 874, 1328, 1601
Hin1I GRCGYC 5 cut(s) 54, 180, 485, 606, 1101
Hin1II CATG 8 cut(s) 406, 415, 553, 778, 924, 1018, 1654, 1688
Hin6I GCGC 7 cut(s) 180, 583, 606, 864, 872, 1326, 1599
HinP1I GCGC 7 cut(s) 180, 583, 606, 864, 872, 1326, 1599
HincII GTYRAC 4 cut(s) 10, 1090, 1099, 1294
HindII GTYRAC 4 cut(s) 10, 1090, 1099, 1294
HphI GGTGA 8 cut(s) 278, 304, 508, 1066, 1126, 1177, 1459, 1561
Hpy166II GTNNAC 5 cut(s) 10, 1090, 1099, 1294, 1344
Hpy8I GTNNAC 5 cut(s) 10, 1090, 1099, 1294, 1344
HpyAV CCTTC 5 cut(s) 43, 208, 407, 993, 1402
HpyCH4III ACNGT 3 cut(s) 69, 642, 786
HpyCH4IV ACGT 4 cut(s) 1101, 1236, 1383, 1562
HpyCH4V TGCA 4 cut(s) 635, 732, 978, 1282
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 614, 658
HpyF3I CTNAG 4 cut(s) 301, 695, 1244, 1423
HpySE526I ACGT 4 cut(s) 1101, 1236, 1383, 1562
Hsp92I GRCGYC 5 cut(s) 54, 180, 485, 606, 1101
Hsp92II CATG 8 cut(s) 406, 415, 553, 778, 924, 1018, 1654, 1688
HspAI GCGC 7 cut(s) 180, 583, 606, 864, 872, 1326, 1599
KasI GGCGCC 2 cut(s) 179, 605
Kpn2I TCCGGA 1 cut(s) 681
KpnI GGTACC 1 cut(s) 1625
KspI CCGCGG 1 cut(s) 1628
Kzo9I GATC 7 cut(s) 232, 268, 373, 741, 1386, 1524, 1587
LmnI GCTCC 4 cut(s) 278, 760, 1314, 1396
Lsp1109I GCAGC 1 cut(s) 1448
LweI GCATC 4 cut(s) 339, 944, 1257, 1716
MaeI CTAG 5 cut(s) 131, 668, 764, 1329, 1535
MaeII ACGT 4 cut(s) 1101, 1236, 1383, 1562
MaeIII GTNAC 6 cut(s) 15, 63, 856, 1183, 1349, 1656
MalI GATC 7 cut(s) 234, 270, 375, 743, 1388, 1526, 1589
MbiI CCGCTC 1 cut(s) 112
MboI GATC 7 cut(s) 232, 268, 373, 741, 1386, 1524, 1587
MboII GAAGA 5 cut(s) 89, 686, 1125, 1243, 1564
MflI RGATCY 2 cut(s) 232, 741
MhlI GDGCHC 5 cut(s) 429, 540, 701, 1128, 1380
MlsI TGGCCA 1 cut(s) 829
MluCI AATT 8 cut(s) 305, 357, 368, 383, 799, 1319, 1636, 1674
MluI ACGCGT 1 cut(s) 1543
MluNI TGGCCA 1 cut(s) 829
Mly113I GGCGCC 2 cut(s) 180, 606
MmeI TCCRAC 4 cut(s) 274, 908, 921, 1090
Mox20I TGGCCA 1 cut(s) 829
MroI TCCGGA 1 cut(s) 681
MscI TGGCCA 1 cut(s) 829
MseI TTAA 5 cut(s) 159, 746, 815, 1485, 1608
MslI CAYNNNNRTG 1 cut(s) 1004
Msp20I TGGCCA 1 cut(s) 829
MspA1I CMGCKG 3 cut(s) 332, 903, 1627
MvnI CGCG 7 cut(s) 585, 866, 1445, 1545, 1586, 1601, 1627
MwoI GCNNNNNNNGC 3 cut(s) 140, 614, 658
NarI GGCGCC 2 cut(s) 180, 606
NdeII GATC 7 cut(s) 232, 268, 373, 741, 1386, 1524, 1587
NheI GCTAGC 2 cut(s) 763, 1328
NlaIII CATG 8 cut(s) 406, 415, 553, 778, 924, 1018, 1654, 1688
NlaIV GGNNCC 7 cut(s) 181, 607, 644, 1078, 1193, 1206, 1623
NmeAIII GCCGAG 5 cut(s) 9, 153, 582, 1181, 1394
NmuCI GTSAC 3 cut(s) 15, 856, 1183
NspI RCATGY 2 cut(s) 924, 1688
PaeR7I CTCGAG 2 cut(s) 578, 1591
PagI TCATGA 1 cut(s) 411
PcsI WCGNNNNNNNCGW 6 cut(s) 147, 471, 1092, 1101, 1504, 1598
PfeI GAWTC 1 cut(s) 1478
PflFI GACNNNGTC 1 cut(s) 1094
PkrI GCNGC 9 cut(s) 179, 380, 494, 572, 905, 1064, 1067, 1463, 1533
Ple19I CGATCG 1 cut(s) 1527
PluTI GGCGCC 2 cut(s) 183, 609
Ppu21I YACGTR 1 cut(s) 1384
PshBI ATTAAT 1 cut(s) 1608
Psp124BI GAGCTC 2 cut(s) 701, 1128
Psp1406I AACGTT 1 cut(s) 1236
PspN4I GGNNCC 7 cut(s) 181, 607, 644, 1078, 1193, 1206, 1623
PspPI GGNCC 7 cut(s) 32, 448, 502, 684, 1204, 1453, 1529
PspXI VCTCGAGB 1 cut(s) 578
PsuI RGATCY 2 cut(s) 232, 741
PsyI GACNNNGTC 1 cut(s) 1094
PvuI CGATCG 1 cut(s) 1527
PvuII CAGCTG 1 cut(s) 332
RsaI GTAC 9 cut(s) 155, 554, 878, 925, 932, 1121, 1343, 1616, 1623
RsaNI GTAC 9 cut(s) 154, 553, 877, 924, 931, 1120, 1342, 1615, 1622
RseI CAYNNNNRTG 1 cut(s) 1004
Rsr2I CGGWCCG 1 cut(s) 684
RsrII CGGWCCG 1 cut(s) 684
SacI GAGCTC 2 cut(s) 701, 1128
SacII CCGCGG 1 cut(s) 1628
SalI GTCGAC 3 cut(s) 8, 1097, 1292
SaqAI TTAA 5 cut(s) 159, 746, 815, 1485, 1608
SatI GCNGC 9 cut(s) 178, 379, 493, 571, 904, 1063, 1066, 1462, 1532
Sau3AI GATC 7 cut(s) 232, 268, 373, 741, 1386, 1524, 1587
Sau96I GGNCC 7 cut(s) 32, 448, 502, 684, 1204, 1453, 1529
SduI GDGCHC 5 cut(s) 429, 540, 701, 1128, 1380
SfaNI GCATC 4 cut(s) 339, 944, 1257, 1716
SfcI CTRYAG 1 cut(s) 898
SfoI GGCGCC 2 cut(s) 181, 607
Sfr274I CTCGAG 2 cut(s) 578, 1591
Sfr303I CCGCGG 1 cut(s) 1628
SgrBI CCGCGG 1 cut(s) 1628
SgrDI CGTCGACG 1 cut(s) 1097
SinI GGWCC 2 cut(s) 684, 1453
SlaI CTCGAG 2 cut(s) 578, 1591
SmaI CCCGGG 2 cut(s) 430, 1374
SmiMI CAYNNNNRTG 1 cut(s) 1004
SmlI CTYRAG 3 cut(s) 578, 1313, 1591
SmoI CTYRAG 3 cut(s) 578, 1313, 1591
SrfI GCCCGGGC 1 cut(s) 430
Sse9I AATT 8 cut(s) 305, 357, 368, 383, 799, 1319, 1636, 1674
SspDI GGCGCC 2 cut(s) 179, 605
SspMI CTAG 5 cut(s) 131, 668, 764, 1329, 1535
SstI GAGCTC 2 cut(s) 701, 1128
TaaI ACNGT 3 cut(s) 69, 642, 786
TaiI ACGT 4 cut(s) 1104, 1239, 1386, 1565
TaqII GACCGA 2 cut(s) 164, 1280
TasI AATT 8 cut(s) 305, 357, 368, 383, 799, 1319, 1636, 1674
TatI WGTACW 3 cut(s) 876, 1341, 1614
TauI GCSGC 8 cut(s) 180, 381, 495, 573, 906, 1065, 1068, 1534
TfiI GAWTC 1 cut(s) 1478
Tru1I TTAA 5 cut(s) 159, 746, 815, 1485, 1608
Tru9I TTAA 5 cut(s) 159, 746, 815, 1485, 1608
TscAI CASTG 3 cut(s) 791, 1463, 1712
TseFI GTSAC 3 cut(s) 15, 856, 1183
TseI GCWGC 1 cut(s) 1461
Tsp45I GTSAC 3 cut(s) 15, 856, 1183
TspDTI ATGAA 3 cut(s) 61, 428, 763
TspGWI ACGGA 1 cut(s) 620
TspMI CCCGGG 2 cut(s) 428, 1372
TspRI CASTG 3 cut(s) 791, 1463, 1712
Tth111I GACNNNGTC 1 cut(s) 1094
VpaK11BI GGWCC 2 cut(s) 684, 1453
VspI ATTAAT 1 cut(s) 1608
XapI RAATTY 3 cut(s) 305, 1319, 1636
XceI RCATGY 2 cut(s) 924, 1688
XhoI CTCGAG 2 cut(s) 578, 1591
XmaI CCCGGG 2 cut(s) 428, 1372
XmiI GTMKAC 3 cut(s) 9, 1098, 1293
XspI CTAG 5 cut(s) 131, 668, 764, 1329, 1535
ZraI GACGTC 1 cut(s) 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.