Rh6DG058900

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0013876
Physical Location & Seq
Reverse (-)
6056659 .. 6059370
2712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG058900.1

Sequence Viewer

Length: 1440 bp
ATGCCAACGATTAAGGAGGAGTTGGATCGTCGTCAATCACTCTACAACCTCCTCATGCCAGTCATGAACCTTTGTGTGCCTGGATTGGACAAAGGCAAGGGCTTATACTTTCTGTTCGTCAAGTCCGAATCAAAGACTCCGGGCGGACTCTTGGCCCGACCCGTTTTGACCAGCTACTACAAAAGCGAGCATTTCAAGACTCGCCCTTACGATCCCTACAATGTCTACACCAGTCCCAACGAGGCCATCCTCTGCCCCGACTCGTTTCAATCCATGTACACTCAAATGCTCTGTGGCCTCCTCGAGCGCCACCAAGTCCTCCGCCTCGGCGCCGTTTTCGCCTCCGGTCTCCTCCGCGCCATCCGCTTCCTCCAGCTCAACTGGCAGCAACTCGCCGCCGACATCCGCACCGGAACACTCAACCCCAAAATTACTGACCCGGAGTTGAGGGGATGCATGGAGCGGAAACTGAAACCCGACCCTGAACTTGCTGAGTTCGTCGCCGAACAATGCGGGGAGGAGAATTGGCAGGGAATCATAACCCGAATTTGGCCCAGTACAAAATATCTCGACGTCATCGTCACCGGAGCTATGGCCCAGTATATTCCGACTTTGGATTACTACAGCGGCGGATTACCGATGGCCTGCACCATGTACGCTTCGTCCGAGTGTTACTTCGGACTCAACCTGAACCCGATGTGCAGACCTTCAGAAGTTTCATACACAATTATGCCAAACATGGCTTACTTCGAGTTCCTCCCCTACGAGCCCAACTCAGTGAGTCTTGACCCGGCCCGACTCGTTGACCTGGTCGACGTGGTGGTCGGAAAAGAGTACGAGTTAGTTATTACCACTTACGCCGGACTGTACCGCTACCGCGTCGGCGACATTCTCCGGGTCACCGGGTTCCACAACTCGGCGCCGCAGTTCCACTTCGTGCGGCGGAAGAACGTCCTGCTCAGCATCGACTCCGACAAGACCGACGAGGCGGAGCTCCAGCTCGCCGTCGACAACGCGACCAAGCTCGTCCTGGAGAAGCTCAACGCCAGCGTGGTGGAGTACACCAGCTTCGCCGACACCAAGACCATCCCGGGCCACTACGTCATCTACTGGGAGCTTCTAGTCAAGGACTCGGCCGAGTCGCCCAGCTCGGAATCGCTGAGTCAGTGCTGCTTGGCCATGGAGGAATCGCTCAACTCGGTTTACCGGCAGTGCCGGGTGGCGGACAACTCGATCGGGCCGTTGGAGATACGTGTGGTGAAGAGCGGCACGTTCGAGGAGTTGATGGACTACGCGATCTCGAGAGGCGCGTCGATTAACCAGTATAAGGTGCCGCGGTGTGTGAATTTTACCCCGATAATGGAGTTACTGGACTCCAGGGTGGTTTCCAAGCATTTTAGCCCTTCATTACCGCATTGGACCCCGGAAAGGAGACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GH3

Protein Analysis

479

Amino Acids

54.37

Weight (kDa)

5.79

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH3 PF03321 1 - 214 8.2e-76 GH3 family N-terminal domain
GH3_M PF23571 241 - 315 1.8e-30 GH3 family central domain
GH3_C PF23572 331 - 450 3.9e-40 GH3 family C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013876)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14960
fragaria_vesca FvH4_2g04750
malus_domestica MD05G1092300.v1.1 MD05G1092900.v1.1
prunus_persica Prupe.8G137900_v2.0.a1
pyrus_communis pycom05g09190 pycom05g09210
rosa_chinensis RchiOBHm_Chr6g0254401
rosa_laevigata RLG00000014915
rosa_multiflora Rmu_ssc0000481.1_g000001
rosa_roxburghii Rroxscaffold_7G00210830
rosa_rugosa Rorug05G0552100
rosa_samantha Rh6AG069100 Rh6BG061700 Rh6CG061900 Rh6DG058900
rosa_wichuraiana Rw6G006070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 578, 821
AatII GACGTC 1 cut(s) 576
AccB1I GGYRCC 3 cut(s) 329, 919, 1330
AccBSI CCGCTC 2 cut(s) 463, 1266
AccI GTMKAC 3 cut(s) 225, 813, 1008
AccII CGCG 6 cut(s) 357, 879, 1016, 1295, 1310, 1336
AclWI GGATC 2 cut(s) 33, 206
AcoI YGGCCR 2 cut(s) 1134, 1176
AcsI RAATTY 2 cut(s) 546, 1345
AcuI CTGAAG 1 cut(s) 693
AcyI GRCGYC 3 cut(s) 330, 573, 920
AdeI CACNNNGTG 1 cut(s) 937
AfaI GTAC 6 cut(s) 278, 559, 656, 836, 869, 1061
AfiI CCNNNNNNNGG 6 cut(s) 549, 916, 1222, 1327, 1360, 1428
AflIII ACRYGT 1 cut(s) 1252
AgsI TTSAA 2 cut(s) 196, 269
AjiI CACGTC 1 cut(s) 817
AjnI CCWGG 4 cut(s) 79, 807, 1029, 1376
Alw21I GWGCWC 1 cut(s) 996
Alw26I GTCTC 2 cut(s) 353, 1426
AlwI GGATC 2 cut(s) 33, 206
Ama87I CYCGRG 3 cut(s) 302, 1090, 1300
ApeKI GCWGC 2 cut(s) 385, 1170
ApoI RAATTY 2 cut(s) 546, 1345
AspLEI GCGC 5 cut(s) 309, 332, 359, 922, 1310
AspS9I GGNCC 7 cut(s) 154, 552, 595, 793, 1093, 1238, 1419
AsuC2I CCSGG 9 cut(s) 141, 440, 791, 896, 904, 1091, 1092, 1217, 1424
AsuHPI GGTGA 3 cut(s) 574, 892, 1270
AvaI CYCGRG 3 cut(s) 302, 1090, 1300
AvaII GGWCC 1 cut(s) 1419
BalI TGGCCA 1 cut(s) 1178
BanI GGYRCC 3 cut(s) 329, 919, 1330
BanII GRGCYC 2 cut(s) 771, 996
BarI GAAGNNNNNNTAC 2 cut(s) 1052, 1084
Bbv12I GWGCWC 1 cut(s) 996
BbvI GCAGC 2 cut(s) 397, 1157
BccI CCATC 5 cut(s) 254, 368, 634, 1094, 1279
BceAI ACGGC 3 cut(s) 317, 989, 1225
BciT130I CCWGG 4 cut(s) 81, 809, 1031, 1378
BcnI CCSGG 9 cut(s) 141, 440, 791, 896, 904, 1091, 1092, 1217, 1424
BcoDI GTCTC 2 cut(s) 353, 1426
BfaI CTAG 1 cut(s) 1121
BfmI CTRYAG 1 cut(s) 622
BfoI RGCGCY 3 cut(s) 310, 333, 923
BisI GCNGC 8 cut(s) 386, 396, 628, 923, 941, 1171, 1267, 1334
BlpI GCTNAGC 1 cut(s) 959
BlsI GCNGC 8 cut(s) 387, 397, 629, 924, 942, 1172, 1268, 1335
Bme18I GGWCC 1 cut(s) 1419
BmeT110I CYCGRG 3 cut(s) 302, 1090, 1300
BmgBI CACGTC 1 cut(s) 817
BmgT120I GGNCC 7 cut(s) 154, 552, 595, 793, 1093, 1238, 1419
BmiI GGNNCC 5 cut(s) 331, 908, 921, 1332, 1421
BmrI ACTGGG 3 cut(s) 549, 592, 1120
BmsI GCATC 2 cut(s) 443, 972
BmuI ACTGGG 3 cut(s) 549, 592, 1120
BplI GAGNNNNNCTC 2 cut(s) 758, 790
BpmI CTGGAG 4 cut(s) 356, 980, 1052, 1360
Bpu1102I GCTNAGC 1 cut(s) 959
BpuMI CCSGG 9 cut(s) 141, 440, 791, 896, 904, 1091, 1092, 1217, 1424
BsaAI YACGTR 1 cut(s) 1253
BsaHI GRCGYC 3 cut(s) 330, 573, 920
BsaI GGTCTC 1 cut(s) 353
BsaJI CCNNGG 6 cut(s) 325, 1090, 1179, 1334, 1377, 1422
BsaWI WCCGGW 3 cut(s) 344, 410, 584
Bsc4I CCNNNNNNNGG 6 cut(s) 549, 916, 1222, 1327, 1360, 1428
Bse118I RCCGGY 1 cut(s) 1206
Bse1I ACTGG 8 cut(s) 59, 231, 386, 555, 598, 1115, 1321, 1374
BseBI CCWGG 4 cut(s) 81, 809, 1031, 1378
BseDI CCNNGG 6 cut(s) 325, 1090, 1179, 1334, 1377, 1422
BseGI GGATG 5 cut(s) 246, 360, 402, 458, 1086
BseLI CCNNNNNNNGG 6 cut(s) 549, 916, 1222, 1327, 1360, 1428
BseMII CTCAG 4 cut(s) 483, 789, 973, 1151
BseNI ACTGG 8 cut(s) 59, 231, 386, 555, 598, 1115, 1321, 1374
BseRI GAGGAG 6 cut(s) 32, 41, 290, 341, 533, 1292
BseX3I CGGCCG 1 cut(s) 1134
BseXI GCAGC 2 cut(s) 397, 1157
BseYI CCCAGC 1 cut(s) 1145
BsgI GTGCAG 2 cut(s) 631, 721
Bsh1236I CGCG 6 cut(s) 357, 879, 1016, 1295, 1310, 1336
Bsh1285I CGRYCG 2 cut(s) 1137, 1236
BshNI GGYRCC 3 cut(s) 329, 919, 1330
BsiEI CGRYCG 2 cut(s) 1137, 1236
BsiHKAI GWGCWC 1 cut(s) 996
BsiHKCI CYCGRG 3 cut(s) 302, 1090, 1300
BslFI GGGAC 1 cut(s) 219
BslI CCNNNNNNNGG 6 cut(s) 549, 916, 1222, 1327, 1360, 1428
BsmAI GTCTC 2 cut(s) 353, 1426
BsmBI CGTCTC 1 cut(s) 1426
BsmFI GGGAC 1 cut(s) 219
Bso31I GGTCTC 1 cut(s) 353
BsoBI CYCGRG 3 cut(s) 302, 1090, 1300
Bsp1286I GDGCHC 2 cut(s) 771, 996
Bsp1407I TGTACA 1 cut(s) 276
Bsp143I GATC 4 cut(s) 25, 211, 1233, 1296
Bsp1720I GCTNAGC 1 cut(s) 959
Bsp19I CCATGG 1 cut(s) 1179
BspCNI CTCAG 4 cut(s) 484, 788, 972, 1152
BspFNI CGCG 6 cut(s) 357, 879, 1016, 1295, 1310, 1336
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 5 cut(s) 331, 908, 921, 1332, 1421
BspPI GGATC 2 cut(s) 33, 206
BspQI GCTCTTC 1 cut(s) 1256
BspT107I GGYRCC 3 cut(s) 329, 919, 1330
BspTNI GGTCTC 1 cut(s) 353
BsrBI CCGCTC 2 cut(s) 463, 1266
BsrFI RCCGGY 1 cut(s) 1206
BsrGI TGTACA 1 cut(s) 276
BsrI ACTGG 8 cut(s) 59, 231, 386, 555, 598, 1115, 1321, 1374
BssAI RCCGGY 1 cut(s) 1206
BssECI CCNNGG 6 cut(s) 325, 1090, 1179, 1334, 1377, 1422
BssMI GATC 4 cut(s) 25, 211, 1233, 1296
BssNI GRCGYC 3 cut(s) 330, 573, 920
BssT1I CCWWGG 1 cut(s) 1179
Bst2UI CCWGG 4 cut(s) 81, 809, 1031, 1378
Bst4CI ACNGT 2 cut(s) 867, 1437
Bst6I CTCTTC 1 cut(s) 1256
BstACI GRCGYC 3 cut(s) 330, 573, 920
BstAUI TGTACA 1 cut(s) 276
BstBAI YACGTR 1 cut(s) 1253
BstC8I GCNNGC 4 cut(s) 188, 646, 1002, 1048
BstDEI CTNAG 4 cut(s) 492, 775, 959, 1160
BstDSI CCRYGG 2 cut(s) 1179, 1334
BstEII GGTNACC 1 cut(s) 898
BstF5I GGATG 5 cut(s) 246, 360, 402, 458, 1086
BstFNI CGCG 6 cut(s) 357, 879, 1016, 1295, 1310, 1336
BstH2I RGCGCY 3 cut(s) 310, 333, 923
BstHHI GCGC 5 cut(s) 309, 332, 359, 922, 1310
BstKTI GATC 4 cut(s) 28, 214, 1236, 1299
BstMAI GTCTC 2 cut(s) 353, 1426
BstMBI GATC 4 cut(s) 25, 211, 1233, 1296
BstMCI CGRYCG 2 cut(s) 1137, 1236
BstMWI GCNNNNNNNGC 3 cut(s) 338, 363, 382
BstNI CCWGG 4 cut(s) 81, 809, 1031, 1378
BstPI GGTNACC 1 cut(s) 898
BstSFI CTRYAG 1 cut(s) 622
BstUI CGCG 6 cut(s) 357, 879, 1016, 1295, 1310, 1336
BstV1I GCAGC 2 cut(s) 397, 1157
BstXI CCANNNNNNTGG 1 cut(s) 1054
BstZI CGGCCG 1 cut(s) 1134
BtgI CCRYGG 2 cut(s) 1179, 1334
BtrI CACGTC 1 cut(s) 817
BtsCI GGATG 5 cut(s) 246, 360, 402, 458, 1086
BtsI GCAGTG 1 cut(s) 1217
BtsIMutI CAGTG 3 cut(s) 783, 1172, 1217
Cac8I GCNNGC 4 cut(s) 188, 646, 1002, 1048
CciI TCATGA 1 cut(s) 63
CfoI GCGC 5 cut(s) 309, 332, 359, 922, 1310
Cfr10I RCCGGY 1 cut(s) 1206
Cfr13I GGNCC 7 cut(s) 154, 552, 595, 793, 1093, 1238, 1419
Cfr42I CCGCGG 1 cut(s) 1337
Cfr9I CCCGGG 1 cut(s) 1090
CseI GACGC 2 cut(s) 868, 1299
CsiI ACCWGGT 1 cut(s) 807
Csp6I GTAC 6 cut(s) 277, 558, 655, 835, 868, 1060
CviAII CATG 7 cut(s) 55, 64, 274, 457, 652, 739, 1180
CviQI GTAC 6 cut(s) 277, 558, 655, 835, 868, 1060
DdeI CTNAG 4 cut(s) 492, 775, 959, 1160
DinI GGCGCC 2 cut(s) 331, 921
DpnI GATC 4 cut(s) 27, 213, 1235, 1298
DpnII GATC 4 cut(s) 25, 211, 1233, 1296
DraIII CACNNNGTG 1 cut(s) 937
DrdI GACNNNNNNGTC 2 cut(s) 578, 821
DseDI GACNNNNNNGTC 2 cut(s) 578, 821
EaeI YGGCCR 2 cut(s) 1134, 1176
EagI CGGCCG 1 cut(s) 1134
Eam1104I CTCTTC 1 cut(s) 1256
EarI CTCTTC 1 cut(s) 1256
EciI GGCGGA 6 cut(s) 159, 311, 645, 958, 1004, 1238
Ecl136II GAGCTC 1 cut(s) 994
EclXI CGGCCG 1 cut(s) 1134
Eco130I CCWWGG 1 cut(s) 1179
Eco24I GRGCYC 2 cut(s) 771, 996
Eco31I GGTCTC 1 cut(s) 353
Eco47I GGWCC 1 cut(s) 1419
Eco52I CGGCCG 1 cut(s) 1134
Eco53kI GAGCTC 1 cut(s) 994
Eco57I CTGAAG 1 cut(s) 693
Eco88I CYCGRG 3 cut(s) 302, 1090, 1300
Eco91I GGTNACC 1 cut(s) 898
EcoICRI GAGCTC 1 cut(s) 994
EcoO65I GGTNACC 1 cut(s) 898
EcoRII CCWGG 4 cut(s) 79, 807, 1029, 1376
EcoT14I CCWWGG 1 cut(s) 1179
EcoT22I ATGCAT 1 cut(s) 458
EcoT38I GRGCYC 2 cut(s) 771, 996
EgeI GGCGCC 2 cut(s) 331, 921
EheI GGCGCC 2 cut(s) 331, 921
ErhI CCWWGG 1 cut(s) 1179
Esp3I CGTCTC 1 cut(s) 1426
FaeI CATG 7 cut(s) 58, 67, 277, 460, 655, 742, 1183
FaqI GGGAC 1 cut(s) 219
FatI CATG 7 cut(s) 54, 63, 273, 456, 651, 738, 1179
FauI CCCGC 1 cut(s) 506
FblI GTMKAC 3 cut(s) 225, 813, 1008
Fnu4HI GCNGC 8 cut(s) 386, 396, 628, 923, 941, 1171, 1267, 1334
FokI GGATG 5 cut(s) 233, 347, 389, 465, 1073
FriOI GRGCYC 2 cut(s) 771, 996
Fsp4HI GCNGC 8 cut(s) 386, 396, 628, 923, 941, 1171, 1267, 1334
FspBI CTAG 1 cut(s) 1121
GlaI GCGC 5 cut(s) 308, 331, 358, 921, 1309
GluI GCNGC 8 cut(s) 386, 396, 628, 923, 941, 1171, 1267, 1334
GsaI CCCAGC 1 cut(s) 1149
GsuI CTGGAG 4 cut(s) 356, 980, 1052, 1360
HaeII RGCGCY 3 cut(s) 310, 333, 923
HgaI GACGC 2 cut(s) 868, 1299
HhaI GCGC 5 cut(s) 309, 332, 359, 922, 1310
Hin1I GRCGYC 3 cut(s) 330, 573, 920
Hin1II CATG 7 cut(s) 58, 67, 277, 460, 655, 742, 1183
Hin6I GCGC 5 cut(s) 307, 330, 357, 920, 1308
HinP1I GCGC 5 cut(s) 307, 330, 357, 920, 1308
HincII GTYRAC 3 cut(s) 805, 814, 1009
HindII GTYRAC 3 cut(s) 805, 814, 1009
HphI GGTGA 3 cut(s) 574, 892, 1270
Hpy166II GTNNAC 7 cut(s) 226, 279, 805, 814, 1009, 1062, 1204
Hpy188I TCNGA 8 cut(s) 127, 609, 667, 680, 712, 827, 973, 1153
Hpy188III TCNNGA 6 cut(s) 64, 196, 569, 785, 1300, 1302
Hpy8I GTNNAC 7 cut(s) 226, 279, 805, 814, 1009, 1062, 1204
Hpy99I CGWCG 8 cut(s) 33, 503, 575, 818, 884, 986, 1010, 1315
HpyAV CCTTC 2 cut(s) 717, 1413
HpyCH4III ACNGT 2 cut(s) 867, 1437
HpyCH4IV ACGT 6 cut(s) 573, 816, 951, 1101, 1252, 1271
HpyCH4V TGCA 3 cut(s) 456, 648, 702
HpyF10VI GCNNNNNNNGC 3 cut(s) 338, 363, 382
HpyF3I CTNAG 4 cut(s) 492, 775, 959, 1160
HpySE526I ACGT 6 cut(s) 573, 816, 951, 1101, 1252, 1271
Hsp92I GRCGYC 3 cut(s) 330, 573, 920
Hsp92II CATG 7 cut(s) 58, 67, 277, 460, 655, 742, 1183
HspAI GCGC 5 cut(s) 307, 330, 357, 920, 1308
KasI GGCGCC 2 cut(s) 329, 919
KspI CCGCGG 1 cut(s) 1337
Kzo9I GATC 4 cut(s) 25, 211, 1233, 1296
LguI GCTCTTC 1 cut(s) 1256
LmnI GCTCC 5 cut(s) 460, 587, 991, 999, 1114
Lsp1109I GCAGC 2 cut(s) 397, 1157
LweI GCATC 2 cut(s) 443, 972
MabI ACCWGGT 1 cut(s) 807
MaeI CTAG 1 cut(s) 1121
MaeII ACGT 6 cut(s) 573, 816, 951, 1101, 1252, 1271
MaeIII GTNAC 4 cut(s) 580, 671, 898, 1365
MalI GATC 4 cut(s) 27, 213, 1235, 1298
MbiI CCGCTC 2 cut(s) 463, 1266
MboI GATC 4 cut(s) 25, 211, 1233, 1296
MboII GAAGA 2 cut(s) 958, 1273
MhlI GDGCHC 2 cut(s) 771, 996
MlsI TGGCCA 1 cut(s) 1178
MluCI AATT 5 cut(s) 429, 523, 546, 726, 1345
MluNI TGGCCA 1 cut(s) 1178
Mly113I GGCGCC 2 cut(s) 330, 920
MmeI TCCRAC 4 cut(s) 632, 805, 996, 1224
Mox20I TGGCCA 1 cut(s) 1178
Mph1103I ATGCAT 1 cut(s) 458
MscI TGGCCA 1 cut(s) 1178
MseI TTAA 2 cut(s) 12, 1317
MslI CAYNNNNRTG 2 cut(s) 284, 728
Msp20I TGGCCA 1 cut(s) 1178
MspA1I CMGCKG 2 cut(s) 627, 1336
MvaI CCWGG 4 cut(s) 81, 809, 1031, 1378
MvnI CGCG 6 cut(s) 357, 879, 1016, 1295, 1310, 1336
MwoI GCNNNNNNNGC 3 cut(s) 338, 363, 382
NarI GGCGCC 2 cut(s) 330, 920
NciI CCSGG 9 cut(s) 141, 440, 791, 896, 904, 1091, 1092, 1217, 1424
NcoI CCATGG 1 cut(s) 1179
NdeII GATC 4 cut(s) 25, 211, 1233, 1296
NlaIII CATG 7 cut(s) 58, 67, 277, 460, 655, 742, 1183
NlaIV GGNNCC 5 cut(s) 331, 908, 921, 1332, 1421
NmeAIII GCCGAG 4 cut(s) 306, 896, 1112, 1162
NmuCI GTSAC 2 cut(s) 580, 898
NsiI ATGCAT 1 cut(s) 458
PaeR7I CTCGAG 2 cut(s) 302, 1300
PagI TCATGA 1 cut(s) 63
PciSI GCTCTTC 1 cut(s) 1256
PcsI WCGNNNNNNNCGW 5 cut(s) 123, 576, 663, 1238, 1307
PfeI GAWTC 4 cut(s) 128, 534, 1154, 1187
PflFI GACNNNGTC 1 cut(s) 809
PfoI TCCNGGA 1 cut(s) 1029
PkrI GCNGC 8 cut(s) 387, 397, 629, 924, 942, 1172, 1268, 1335
Ple19I CGATCG 1 cut(s) 1236
PluTI GGCGCC 2 cut(s) 333, 923
Ppu21I YACGTR 1 cut(s) 1253
Psp124BI GAGCTC 1 cut(s) 996
Psp6I CCWGG 4 cut(s) 79, 807, 1029, 1376
PspEI GGTNACC 1 cut(s) 898
PspFI CCCAGC 1 cut(s) 1145
PspGI CCWGG 4 cut(s) 79, 807, 1029, 1376
PspN4I GGNNCC 5 cut(s) 331, 908, 921, 1332, 1421
PspPI GGNCC 7 cut(s) 154, 552, 595, 793, 1093, 1238, 1419
PspXI VCTCGAGB 1 cut(s) 302
PsyI GACNNNGTC 1 cut(s) 809
PvuI CGATCG 1 cut(s) 1236
RsaI GTAC 6 cut(s) 278, 559, 656, 836, 869, 1061
RsaNI GTAC 6 cut(s) 277, 558, 655, 835, 868, 1060
RseI CAYNNNNRTG 2 cut(s) 284, 728
SacI GAGCTC 1 cut(s) 996
SacII CCGCGG 1 cut(s) 1337
SalI GTCGAC 2 cut(s) 812, 1007
SapI GCTCTTC 1 cut(s) 1256
SaqAI TTAA 2 cut(s) 12, 1317
SatI GCNGC 8 cut(s) 386, 396, 628, 923, 941, 1171, 1267, 1334
Sau3AI GATC 4 cut(s) 25, 211, 1233, 1296
Sau96I GGNCC 7 cut(s) 154, 552, 595, 793, 1093, 1238, 1419
SduI GDGCHC 2 cut(s) 771, 996
SexAI ACCWGGT 1 cut(s) 807
SfaNI GCATC 2 cut(s) 443, 972
SfcI CTRYAG 1 cut(s) 622
SfoI GGCGCC 2 cut(s) 331, 921
Sfr274I CTCGAG 2 cut(s) 302, 1300
Sfr303I CCGCGG 1 cut(s) 1337
SgrBI CCGCGG 1 cut(s) 1337
SinI GGWCC 1 cut(s) 1419
SlaI CTCGAG 2 cut(s) 302, 1300
SmaI CCCGGG 1 cut(s) 1092
SmiMI CAYNNNNRTG 2 cut(s) 284, 728
SmlI CTYRAG 2 cut(s) 302, 1300
SmoI CTYRAG 2 cut(s) 302, 1300
Sse9I AATT 5 cut(s) 429, 523, 546, 726, 1345
SspDI GGCGCC 2 cut(s) 329, 919
SspMI CTAG 1 cut(s) 1121
SstI GAGCTC 1 cut(s) 996
StyI CCWWGG 1 cut(s) 1179
TaaI ACNGT 2 cut(s) 867, 1437
TaiI ACGT 6 cut(s) 576, 819, 954, 1104, 1255, 1274
TaqII GACCGA 1 cut(s) 995
TasI AATT 5 cut(s) 429, 523, 546, 726, 1345
TatI WGTACW 3 cut(s) 276, 557, 1059
TauI GCSGC 6 cut(s) 398, 630, 925, 943, 1269, 1336
TfiI GAWTC 4 cut(s) 128, 534, 1154, 1187
Tru1I TTAA 2 cut(s) 12, 1317
Tru9I TTAA 2 cut(s) 12, 1317
TscAI CASTG 3 cut(s) 783, 1172, 1217
TseFI GTSAC 2 cut(s) 580, 898
TseI GCWGC 2 cut(s) 385, 1170
Tsp45I GTSAC 2 cut(s) 580, 898
TspDTI ATGAA 3 cut(s) 80, 708, 1395
TspMI CCCGGG 1 cut(s) 1090
TspRI CASTG 3 cut(s) 783, 1172, 1217
Tth111I GACNNNGTC 1 cut(s) 809
VpaK11BI GGWCC 1 cut(s) 1419
XapI RAATTY 2 cut(s) 546, 1345
XcmI CCANNNNNNNNNTGG 1 cut(s) 1027
XhoI CTCGAG 2 cut(s) 302, 1300
XmaI CCCGGG 1 cut(s) 1090
XmiI GTMKAC 3 cut(s) 225, 813, 1008
XspI CTAG 1 cut(s) 1121
ZraI GACGTC 1 cut(s) 574
Zsp2I ATGCAT 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.