pycom05g09190

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013876
Physical Location & Seq
Forward (+)
12240228 .. 12242641
2414 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g09190.4

Sequence Viewer

Length: 2043 bp
ATGCCAGCAGACTTCCCCATCCCTCCCTCCCAGCACCGCCTCCACCCCCTTTATAAAGCCCTCCAAACCCGGTCCTCAATCCCACTGCACCAAACACAATCCTCCTCGATCTCTCTCTTTCCTCTTCCCCTCACTGCCTTCTCCTCTCTGTTTATAACCAACACGCAACAAAAGACCCTTCATTTCAAATTAACTTACGGTCCTCTCGCCACTCCAAAATCTTATCTTCAAATGGCTGTCGACTCCTCACTCTCTCCCCTCGGCCCACCGGCCTGCGACAAGGACGCCAAAGCGTTACAGTTCATTGAAGAAACTACCCGGAATGCCGACGCCGTCCAAGAGCGGGTGCTGTCCGAGATACTAGCTCGAAACGCCCAGACCGAGTACCTTAAGCGCTTCGAACTCGGCGGCGCCACCGACCGCAAAACCTTCAAGTCCAAACTCCCCGTGATTACCTACGAGGATCGATCCATCCCGAAATCCAACGCATTGCCAATGATCCGAGATCGCTCTCCCATCTTGTCAGCTCATCCCATCTCAGAATTCCTCACCAGTTCTGGGACTTCAGCTGGAGAGAGGAAGCTGATGCCAACAATTCAGGAGGAATTGGATCGCCGCCACTTATTATACAGCCTTCTCATGCCCGTCATGAACCTTTGTGTGCCCGGGCTGGACAAAGGAAAGGGCCTATACTTTCTGTTCGTGAAGTCCGAAACAAAGACGCCGGGCGGGCTTTTGGCCCGACCCGTTCTCACCAGCTACTACAAAAGCGAGCACTTCAAGACGCGCCCGTACGACCCGTACAACGTGTACACGAGTCCGAATGAGGCAATCCTTTGCCCGGACTCCTTCCAGAGCATGTACGCCCAAATGCTCTGCGGCCTCCTCGAGCGCGAAAAGGTCCTCCGCCTCGGCGCCGTTTTTGCGTCCGGTCTCCTCCGTGCAATACGGTTCCTCCAGCTCAACTGGCAACAACTAGCAGAAGACATCCGGACCGGAACTCTCAGCTCGAAAATAACTGACCCGGATTTGAAATCTTGCATAGGCGGGATCTTAGAACCCGACCCGGAGCTAGCGGATTTCATGTCCAAACAGTGCGGAAAAGAAAATTGGGAAGGGATTTTAACTCGGATATGGCCAAACACGAAATATCTGGACGTGATAGTGACTGGCGCGATGGCGCAGTACATTCCGACGTTGGATTACTACAGCGGCGGATTGCCCATGGCATGTACGATGTACGCATCGTCCGAGTGTTATTTTGGACTCAATCTCAACCCGATGTGCAAGCCTTCCGAAGTTTCCTACACGATAATGCCAAACATGGCCTATTTCGAGTTCCTCCCTTACGAGCCAAACTCGGTGAGTGGCGGCGGCGAGTCGGGAACCAGACTCGTTGACCTCGTCGAGGTCGAGGTCGGAAGAGAGTACGAGCTCGTAATCACCACCTACGCCGGGCTTCACCGATACAGAGTCGGGGACATCCTCCGAGTCACCGGATTCCACAACTCGGCCCCGCAGTTCCACTTCGTGAGGAGGAAGAACGTTTTGCTCAGCATCGATTCGGACAAGACCGACGAAGCAGAGTTGCAACTCGCTGTCGACAACGCCTCCAAGCTCCTCAAGAAATTTAGCGCTAGCGTGGTGGAGTACACGAGTCACGCCGACACGACGACAATCCCGGGGCACTACGTGATCTATTGGGAGCTTCTCGTGAAGGACTCGACGAACTCAGAGAGCGAATCCTTCGACTCAGTGATTGACCAGTGCTGCCTAACGATGGAGGAATCACTTAACTCGGTGTATCGGCAGGGTCGAGTGGCGGACAACTCGATCGGGCCGCTAGAGATACGCGTGGTGAAGAACGGCACGTTTGAGGAGTTGATGGACTACGCGATCTCGAGAGGCGCGTCGATTAATCAGTACAAGGTACCGCGGTGTGTGAATTTTACCCCAATCATGGAGTTACTGGACTCCAGGGTAATTTCCAAGCATATCAGCCCATCTTTGCCGCACTGGACTCCTGAAAGGAAGATGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GH3

Protein Analysis

681

Amino Acids

76.38

Weight (kDa)

6.43

Isoelectric Point (pI)

45.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013876)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14960
fragaria_vesca FvH4_2g04750
malus_domestica MD05G1092300.v1.1 MD05G1092900.v1.1
prunus_persica Prupe.8G137900_v2.0.a1
pyrus_communis pycom05g09190 pycom05g09210
rosa_chinensis RchiOBHm_Chr6g0254401
rosa_laevigata RLG00000014915
rosa_multiflora Rmu_ssc0000481.1_g000001
rosa_roxburghii Rroxscaffold_7G00210830
rosa_rugosa Rorug05G0552100
rosa_samantha Rh6AG069100 Rh6BG061700 Rh6CG061900 Rh6DG058900
rosa_wichuraiana Rw6G006070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 54, 155
Acc65I GGTACC 1 cut(s) 1930
AccB1I GGYRCC 3 cut(s) 410, 914, 1930
AccBSI CCGCTC 1 cut(s) 343
AccI GTMKAC 2 cut(s) 240, 1602
AccII CGCG 7 cut(s) 787, 894, 1175, 1854, 1895, 1910, 1936
AccIII TCCGGA 1 cut(s) 990
AclI AACGTT 1 cut(s) 1545
AclWI GGATC 5 cut(s) 462, 471, 493, 618, 1058
AcoI YGGCCR 1 cut(s) 1136
AcsI RAATTY 3 cut(s) 542, 1628, 1945
AcuI CTGAAG 1 cut(s) 549
AcyI GRCGYC 5 cut(s) 285, 330, 411, 722, 915
AdeI CACNNNGTG 2 cut(s) 1531, 1693
AfeI AGCGCT 2 cut(s) 395, 1636
AfiI CCNNNNNNNGG 8 cut(s) 343, 558, 841, 1408, 1455, 1510, 1780, 1960
AflII CTTAAG 1 cut(s) 389
AflIII ACRYGT 2 cut(s) 807, 1852
AgsI TTSAA 6 cut(s) 187, 230, 308, 433, 781, 1033
AjiI CACGTC 1 cut(s) 1159
AjnI CCWGG 1 cut(s) 1976
AjuI GAANNNNNNNTTGG 2 cut(s) 1093, 1125
Alw21I GWGCWC 2 cut(s) 777, 1437
Alw26I GTCTC 1 cut(s) 938
AlwI GGATC 5 cut(s) 462, 471, 493, 618, 1058
Ama87I CYCGRG 4 cut(s) 665, 887, 1681, 1900
Aor13HI TCCGGA 1 cut(s) 990
Aor51HI AGCGCT 2 cut(s) 395, 1636
AoxI GGCC 9 cut(s) 262, 270, 685, 738, 880, 1136, 1326, 1512, 1838
ApeKI GCWGC 1 cut(s) 1770
ApoI RAATTY 3 cut(s) 542, 1628, 1945
AseI ATTAAT 1 cut(s) 1917
Asp718I GGTACC 1 cut(s) 1930
AspLEI GCGC 9 cut(s) 396, 413, 789, 894, 917, 1175, 1183, 1637, 1910
AspS9I GGNCC 9 cut(s) 72, 200, 263, 685, 739, 901, 993, 1513, 1838
AsuHPI GGTGA 7 cut(s) 541, 745, 1375, 1435, 1454, 1486, 1870
AsuII TTCGAA 1 cut(s) 399
AsuNHI GCTAGC 2 cut(s) 1072, 1637
AvaI CYCGRG 4 cut(s) 665, 887, 1681, 1900
AvaII GGWCC 4 cut(s) 72, 200, 901, 993
BaeGI GKGCMC 2 cut(s) 666, 1689
BaeI ACNNNNGTAYC 2 cut(s) 1922, 1955
BalI TGGCCA 1 cut(s) 1138
BanI GGYRCC 3 cut(s) 410, 914, 1930
BanII GRGCYC 1 cut(s) 1437
BauI CACGAG 3 cut(s) 814, 1654, 1712
BbsI GAAGAC 1 cut(s) 990
Bbv12I GWGCWC 2 cut(s) 777, 1437
BbvI GCAGC 1 cut(s) 1757
BccI CCATC 8 cut(s) 26, 479, 524, 542, 1171, 1774, 1879, 2011
BceAI ACGGC 3 cut(s) 317, 902, 1882
BciT130I CCWGG 1 cut(s) 1978
BcoDI GTCTC 1 cut(s) 938
BfaI CTAG 5 cut(s) 362, 977, 1073, 1638, 1844
BfmI CTRYAG 1 cut(s) 1207
BfoI RGCGCY 4 cut(s) 397, 414, 918, 1638
BfrI CTTAAG 1 cut(s) 389
BglI GCCNNNNNGGC 1 cut(s) 730
BisI GCNGC 9 cut(s) 409, 616, 880, 1213, 1372, 1375, 1771, 1841, 2012
BlpI GCTNAGC 1 cut(s) 1553
BlsI GCNGC 9 cut(s) 410, 617, 881, 1214, 1373, 1376, 1772, 1842, 2013
Bme18I GGWCC 4 cut(s) 72, 200, 901, 993
BmeT110I CYCGRG 4 cut(s) 665, 887, 1681, 1900
BmgBI CACGTC 1 cut(s) 1159
BmgT120I GGNCC 9 cut(s) 72, 200, 263, 685, 739, 901, 993, 1513, 1838
BmiI GGNNCC 6 cut(s) 412, 916, 953, 1387, 1515, 1932
BmsI GCATC 4 cut(s) 576, 1253, 1566, 2025
BmtI GCTAGC 2 cut(s) 1076, 1641
BpiI GAAGAC 1 cut(s) 990
BplI GAGNNNNNCTC 2 cut(s) 1343, 1375
BpmI CTGGAG 3 cut(s) 591, 941, 1960
Bpu1102I GCTNAGC 1 cut(s) 1553
Bpu14I TTCGAA 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 1607
Bsa29I ATCGAT 2 cut(s) 466, 1560
BsaAI YACGTR 1 cut(s) 1693
BsaHI GRCGYC 5 cut(s) 285, 330, 411, 722, 915
BsaI GGTCTC 1 cut(s) 938
BsaJI CCNNGG 8 cut(s) 259, 665, 910, 1224, 1681, 1682, 1934, 1977
BsaWI WCCGGW 4 cut(s) 929, 990, 995, 1496
BsaXI ACNNNNNCTCC 4 cut(s) 939, 969, 1595, 1625
Bsc4I CCNNNNNNNGG 8 cut(s) 343, 558, 841, 1408, 1455, 1510, 1780, 1960
Bse118I RCCGGY 1 cut(s) 268
Bse1I ACTGG 6 cut(s) 552, 971, 1174, 1765, 1974, 2021
Bse3DI GCAATG 1 cut(s) 488
BseAI TCCGGA 1 cut(s) 990
BseBI CCWGG 1 cut(s) 1978
BseCI ATCGAT 2 cut(s) 466, 1560
BseDI CCNNGG 8 cut(s) 259, 665, 910, 1224, 1681, 1682, 1934, 1977
BseGI GGATG 5 cut(s) 18, 471, 529, 987, 1482
BseLI CCNNNNNNNGG 8 cut(s) 343, 558, 841, 1408, 1455, 1510, 1780, 1960
BseMI GCAATG 1 cut(s) 488
BseMII CTCAG 5 cut(s) 552, 1018, 1567, 1746, 1767
BseNI ACTGG 6 cut(s) 552, 971, 1174, 1765, 1974, 2021
BseRI GAGGAG 8 cut(s) 94, 133, 235, 875, 926, 1549, 1610, 1892
BseSI GKGCMC 2 cut(s) 666, 1689
BseXI GCAGC 1 cut(s) 1757
BseYI CCCAGC 1 cut(s) 30
BsgI GTGCAG 1 cut(s) 71
Bsh1236I CGCG 7 cut(s) 787, 894, 1175, 1854, 1895, 1910, 1936
Bsh1285I CGRYCG 2 cut(s) 421, 1836
BshFI GGCC 9 cut(s) 264, 272, 687, 740, 882, 1138, 1328, 1514, 1840
BshNI GGYRCC 3 cut(s) 410, 914, 1930
BshVI ATCGAT 2 cut(s) 466, 1560
BsiEI CGRYCG 2 cut(s) 421, 1836
BsiHKAI GWGCWC 2 cut(s) 777, 1437
BsiHKCI CYCGRG 4 cut(s) 665, 887, 1681, 1900
BsiWI CGTACG 1 cut(s) 792
BslFI GGGAC 2 cut(s) 574, 1493
BslI CCNNNNNNNGG 8 cut(s) 343, 558, 841, 1408, 1455, 1510, 1780, 1960
BsmAI GTCTC 1 cut(s) 938
BsmFI GGGAC 2 cut(s) 574, 1493
BsmI GAATGC 1 cut(s) 328
BsnI GGCC 9 cut(s) 264, 272, 687, 740, 882, 1138, 1328, 1514, 1840
Bso31I GGTCTC 1 cut(s) 938
BsoBI CYCGRG 4 cut(s) 665, 887, 1681, 1900
Bsp119I TTCGAA 1 cut(s) 399
Bsp1286I GDGCHC 4 cut(s) 666, 777, 1437, 1689
Bsp13I TCCGGA 1 cut(s) 990
Bsp1407I TGTACA 1 cut(s) 810
Bsp1720I GCTNAGC 1 cut(s) 1553
Bsp19I CCATGG 1 cut(s) 1224
BspANI GGCC 9 cut(s) 264, 272, 687, 740, 882, 1138, 1328, 1514, 1840
BspCNI CTCAG 5 cut(s) 551, 1017, 1566, 1745, 1766
BspDI ATCGAT 2 cut(s) 466, 1560
BspEI TCCGGA 1 cut(s) 990
BspFNI CGCG 7 cut(s) 787, 894, 1175, 1854, 1895, 1910, 1936
BspHI TCATGA 1 cut(s) 648
BspLI GGNNCC 6 cut(s) 412, 916, 953, 1387, 1515, 1932
BspOI GCTAGC 2 cut(s) 1076, 1641
BspPI GGATC 5 cut(s) 462, 471, 493, 618, 1058
BspT104I TTCGAA 1 cut(s) 399
BspT107I GGYRCC 3 cut(s) 410, 914, 1930
BspTI CTTAAG 1 cut(s) 389
BspTNI GGTCTC 1 cut(s) 938
BsrBI CCGCTC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 488
BsrFI RCCGGY 1 cut(s) 268
BsrGI TGTACA 1 cut(s) 810
BsrI ACTGG 6 cut(s) 552, 971, 1174, 1765, 1974, 2021
BssAI RCCGGY 1 cut(s) 268
BssECI CCNNGG 8 cut(s) 259, 665, 910, 1224, 1681, 1682, 1934, 1977
BssNI GRCGYC 5 cut(s) 285, 330, 411, 722, 915
BssSI CACGAG 3 cut(s) 814, 1654, 1712
BssT1I CCWWGG 1 cut(s) 1224
Bst2BI CACGAG 3 cut(s) 814, 1654, 1712
Bst2UI CCWGG 1 cut(s) 1978
Bst4CI ACNGT 4 cut(s) 200, 300, 951, 1095
Bst6I CTCTTC 2 cut(s) 129, 1417
BstACI GRCGYC 5 cut(s) 285, 330, 411, 722, 915
BstAFI CTTAAG 1 cut(s) 389
BstAUI TGTACA 1 cut(s) 810
BstBAI YACGTR 1 cut(s) 1693
BstBI TTCGAA 1 cut(s) 399
BstC8I GCNNGC 7 cut(s) 6, 274, 731, 773, 1074, 1289, 1639
BstDEI CTNAG 6 cut(s) 538, 1004, 1054, 1553, 1732, 1753
BstDSI CCRYGG 2 cut(s) 1224, 1934
BstENI CCTNNNNNAGG 1 cut(s) 1406
BstF5I GGATG 5 cut(s) 18, 471, 529, 987, 1482
BstFNI CGCG 7 cut(s) 787, 894, 1175, 1854, 1895, 1910, 1936
BstH2I RGCGCY 4 cut(s) 397, 414, 918, 1638
BstHHI GCGC 9 cut(s) 396, 413, 789, 894, 917, 1175, 1183, 1637, 1910
BstMAI GTCTC 1 cut(s) 938
BstMCI CGRYCG 2 cut(s) 421, 1836
BstMWI GCNNNNNNNGC 4 cut(s) 371, 730, 923, 967
BstNI CCWGG 1 cut(s) 1978
BstNSI RCATGY 2 cut(s) 862, 1233
BstSFI CTRYAG 1 cut(s) 1207
BstSLI GKGCMC 2 cut(s) 666, 1689
BstUI CGCG 7 cut(s) 787, 894, 1175, 1854, 1895, 1910, 1936
BstV1I GCAGC 1 cut(s) 1757
BstV2I GAAGAC 1 cut(s) 990
BstX2I RGATCY 1 cut(s) 1050
BstYI RGATCY 1 cut(s) 1050
Bsu15I ATCGAT 2 cut(s) 466, 1560
BsuRI GGCC 9 cut(s) 264, 272, 687, 740, 882, 1138, 1328, 1514, 1840
BsuTUI ATCGAT 2 cut(s) 466, 1560
BtgI CCRYGG 2 cut(s) 1224, 1934
BtgZI GCGATG 1 cut(s) 1190
BtrI CACGTC 1 cut(s) 1159
BtsCI GGATG 5 cut(s) 18, 471, 529, 987, 1482
BtsI GCAGTG 2 cut(s) 83, 132
BtsIMutI CAGTG 6 cut(s) 83, 132, 1100, 1761, 1772, 2014
Cac8I GCNNGC 7 cut(s) 6, 274, 731, 773, 1074, 1289, 1639
CciI TCATGA 1 cut(s) 648
CfoI GCGC 9 cut(s) 396, 413, 789, 894, 917, 1175, 1183, 1637, 1910
Cfr10I RCCGGY 1 cut(s) 268
Cfr13I GGNCC 9 cut(s) 72, 200, 263, 685, 739, 901, 993, 1513, 1838
Cfr42I CCGCGG 1 cut(s) 1937
Cfr9I CCCGGG 2 cut(s) 665, 1681
ClaI ATCGAT 2 cut(s) 466, 1560
CpoI CGGWCCG 1 cut(s) 993
CseI GACGC 6 cut(s) 293, 338, 730, 793, 915, 1899
CspI CGGWCCG 1 cut(s) 993
CviAII CATG 8 cut(s) 640, 649, 859, 1084, 1225, 1230, 1324, 1960
DdeI CTNAG 6 cut(s) 538, 1004, 1054, 1553, 1732, 1753
DinI GGCGCC 2 cut(s) 412, 916
DraIII CACNNNGTG 2 cut(s) 1531, 1693
EaeI YGGCCR 1 cut(s) 1136
Eam1104I CTCTTC 2 cut(s) 129, 1417
EarI CTCTTC 2 cut(s) 129, 1417
EciI GGCGGA 3 cut(s) 896, 1230, 1838
Ecl136II GAGCTC 1 cut(s) 1435
Eco130I CCWWGG 1 cut(s) 1224
Eco24I GRGCYC 1 cut(s) 1437
Eco31I GGTCTC 1 cut(s) 938
Eco47I GGWCC 4 cut(s) 72, 200, 901, 993
Eco47III AGCGCT 2 cut(s) 395, 1636
Eco53kI GAGCTC 1 cut(s) 1435
Eco57I CTGAAG 1 cut(s) 549
Eco88I CYCGRG 4 cut(s) 665, 887, 1681, 1900
EcoICRI GAGCTC 1 cut(s) 1435
EcoNI CCTNNNNNAGG 1 cut(s) 1406
EcoO109I RGGNCCY 2 cut(s) 685, 901
EcoRI GAATTC 1 cut(s) 542
EcoRII CCWGG 1 cut(s) 1976
EcoT14I CCWWGG 1 cut(s) 1224
EcoT38I GRGCYC 1 cut(s) 1437
EgeI GGCGCC 2 cut(s) 412, 916
EheI GGCGCC 2 cut(s) 412, 916
ErhI CCWWGG 1 cut(s) 1224
FaeI CATG 8 cut(s) 643, 652, 862, 1087, 1228, 1233, 1327, 1963
FaqI GGGAC 2 cut(s) 574, 1493
FatI CATG 8 cut(s) 639, 648, 858, 1083, 1224, 1229, 1323, 1959
FauI CCCGC 4 cut(s) 336, 722, 1040, 1524
FblI GTMKAC 2 cut(s) 240, 1602
Fnu4HI GCNGC 9 cut(s) 409, 616, 880, 1213, 1372, 1375, 1771, 1841, 2012
FokI GGATG 5 cut(s) 5, 458, 516, 974, 1469
FriOI GRGCYC 1 cut(s) 1437
Fsp4HI GCNGC 9 cut(s) 409, 616, 880, 1213, 1372, 1375, 1771, 1841, 2012
FspBI CTAG 5 cut(s) 362, 977, 1073, 1638, 1844
GlaI GCGC 9 cut(s) 395, 412, 788, 893, 916, 1174, 1182, 1636, 1909
GluI GCNGC 9 cut(s) 409, 616, 880, 1213, 1372, 1375, 1771, 1841, 2012
GsaI CCCAGC 1 cut(s) 34
GsuI CTGGAG 3 cut(s) 591, 941, 1960
HaeII RGCGCY 4 cut(s) 397, 414, 918, 1638
HaeIII GGCC 9 cut(s) 264, 272, 687, 740, 882, 1138, 1328, 1514, 1840
HgaI GACGC 6 cut(s) 293, 338, 730, 793, 915, 1899
HhaI GCGC 9 cut(s) 396, 413, 789, 894, 917, 1175, 1183, 1637, 1910
Hin1I GRCGYC 5 cut(s) 285, 330, 411, 722, 915
Hin1II CATG 8 cut(s) 643, 652, 862, 1087, 1228, 1233, 1327, 1963
Hin6I GCGC 9 cut(s) 394, 411, 787, 892, 915, 1173, 1181, 1635, 1908
HinP1I GCGC 9 cut(s) 394, 411, 787, 892, 915, 1173, 1181, 1635, 1908
HincII GTYRAC 3 cut(s) 241, 1399, 1603
HindII GTYRAC 3 cut(s) 241, 1399, 1603
HphI GGTGA 7 cut(s) 541, 745, 1375, 1435, 1454, 1486, 1870
Hpy166II GTNNAC 6 cut(s) 241, 811, 813, 1399, 1603, 1653
Hpy8I GTNNAC 6 cut(s) 241, 811, 813, 1399, 1603, 1653
Hpy99I CGWCG 7 cut(s) 332, 1198, 1409, 1580, 1675, 1729, 1915
HpyAV CCTTC 9 cut(s) 148, 188, 439, 644, 859, 1109, 1302, 1711, 1756
HpyCH4III ACNGT 4 cut(s) 200, 300, 951, 1095
HpyCH4IV ACGT 6 cut(s) 807, 1158, 1196, 1545, 1692, 1871
HpyCH4V TGCA 5 cut(s) 88, 944, 1041, 1287, 1591
HpyF10VI GCNNNNNNNGC 4 cut(s) 371, 730, 923, 967
HpyF3I CTNAG 6 cut(s) 538, 1004, 1054, 1553, 1732, 1753
HpySE526I ACGT 6 cut(s) 807, 1158, 1196, 1545, 1692, 1871
Hsp92I GRCGYC 5 cut(s) 285, 330, 411, 722, 915
Hsp92II CATG 8 cut(s) 643, 652, 862, 1087, 1228, 1233, 1327, 1963
HspAI GCGC 9 cut(s) 394, 411, 787, 892, 915, 1173, 1181, 1635, 1908
KasI GGCGCC 2 cut(s) 410, 914
Kpn2I TCCGGA 1 cut(s) 990
KpnI GGTACC 1 cut(s) 1934
KspI CCGCGG 1 cut(s) 1937
LmnI GCTCC 3 cut(s) 1069, 1623, 1705
Lsp1109I GCAGC 1 cut(s) 1757
LweI GCATC 4 cut(s) 576, 1253, 1566, 2025
MaeI CTAG 5 cut(s) 362, 977, 1073, 1638, 1844
MaeII ACGT 6 cut(s) 807, 1158, 1196, 1545, 1692, 1871
MaeIII GTNAC 5 cut(s) 294, 1165, 1492, 1658, 1965
MbiI CCGCTC 1 cut(s) 343
MboII GAAGA 7 cut(s) 116, 218, 320, 995, 1434, 1552, 1873
MflI RGATCY 1 cut(s) 1050
MhlI GDGCHC 4 cut(s) 666, 777, 1437, 1689
MlsI TGGCCA 1 cut(s) 1138
MluCI AATT 8 cut(s) 188, 542, 594, 605, 1108, 1628, 1945, 1983
MluI ACGCGT 1 cut(s) 1852
MluNI TGGCCA 1 cut(s) 1138
Mly113I GGCGCC 2 cut(s) 411, 915
MmeI TCCRAC 4 cut(s) 507, 1179, 1217, 1399
Mox20I TGGCCA 1 cut(s) 1138
MroI TCCGGA 1 cut(s) 990
MscI TGGCCA 1 cut(s) 1138
MseI TTAA 5 cut(s) 191, 390, 1124, 1794, 1917
MslI CAYNNNNRTG 1 cut(s) 1313
Msp20I TGGCCA 1 cut(s) 1138
MspA1I CMGCKG 3 cut(s) 569, 1212, 1936
MspCI CTTAAG 1 cut(s) 389
Mva1269I GAATGC 1 cut(s) 328
MvaI CCWGG 1 cut(s) 1978
MvnI CGCG 7 cut(s) 787, 894, 1175, 1854, 1895, 1910, 1936
MwoI GCNNNNNNNGC 4 cut(s) 371, 730, 923, 967
NarI GGCGCC 2 cut(s) 411, 915
NcoI CCATGG 1 cut(s) 1224
NheI GCTAGC 2 cut(s) 1072, 1637
NlaIII CATG 8 cut(s) 643, 652, 862, 1087, 1228, 1233, 1327, 1963
NlaIV GGNNCC 6 cut(s) 412, 916, 953, 1387, 1515, 1932
NmeAIII GCCGAG 4 cut(s) 240, 384, 891, 1490
NmuCI GTSAC 3 cut(s) 1165, 1492, 1658
NspI RCATGY 2 cut(s) 862, 1233
NspV TTCGAA 1 cut(s) 399
PaeR7I CTCGAG 2 cut(s) 887, 1900
PagI TCATGA 1 cut(s) 648
PcsI WCGNNNNNNNCGW 7 cut(s) 378, 708, 1248, 1401, 1410, 1813, 1907
PctI GAATGC 1 cut(s) 328
PfeI GAWTC 4 cut(s) 1500, 1562, 1742, 1787
Pfl23II CGTACG 1 cut(s) 792
PflFI GACNNNGTC 2 cut(s) 332, 1403
PkrI GCNGC 9 cut(s) 410, 617, 881, 1214, 1373, 1376, 1772, 1842, 2013
Ple19I CGATCG 1 cut(s) 1836
PluTI GGCGCC 2 cut(s) 414, 918
Ppu21I YACGTR 1 cut(s) 1693
PpuMI RGGWCCY 1 cut(s) 901
PshBI ATTAAT 1 cut(s) 1917
PsiI TTATAA 2 cut(s) 54, 155
Psp124BI GAGCTC 1 cut(s) 1437
Psp1406I AACGTT 1 cut(s) 1545
Psp5II RGGWCCY 1 cut(s) 901
Psp6I CCWGG 1 cut(s) 1976
PspFI CCCAGC 1 cut(s) 30
PspGI CCWGG 1 cut(s) 1976
PspLI CGTACG 1 cut(s) 792
PspN4I GGNNCC 6 cut(s) 412, 916, 953, 1387, 1515, 1932
PspPI GGNCC 9 cut(s) 72, 200, 263, 685, 739, 901, 993, 1513, 1838
PspPPI RGGWCCY 1 cut(s) 901
PspXI VCTCGAGB 1 cut(s) 887
PsuI RGATCY 1 cut(s) 1050
PsyI GACNNNGTC 2 cut(s) 332, 1403
PvuI CGATCG 1 cut(s) 1836
PvuII CAGCTG 1 cut(s) 569
RseI CAYNNNNRTG 1 cut(s) 1313
Rsr2I CGGWCCG 1 cut(s) 993
RsrII CGGWCCG 1 cut(s) 993
SacI GAGCTC 1 cut(s) 1437
SacII CCGCGG 1 cut(s) 1937
SalI GTCGAC 2 cut(s) 239, 1601
SaqAI TTAA 5 cut(s) 191, 390, 1124, 1794, 1917
SatI GCNGC 9 cut(s) 409, 616, 880, 1213, 1372, 1375, 1771, 1841, 2012
Sau96I GGNCC 9 cut(s) 72, 200, 263, 685, 739, 901, 993, 1513, 1838
SduI GDGCHC 4 cut(s) 666, 777, 1437, 1689
SfaNI GCATC 4 cut(s) 576, 1253, 1566, 2025
SfcI CTRYAG 1 cut(s) 1207
SfoI GGCGCC 2 cut(s) 412, 916
Sfr274I CTCGAG 2 cut(s) 887, 1900
Sfr303I CCGCGG 1 cut(s) 1937
SfuI TTCGAA 1 cut(s) 399
SgrBI CCGCGG 1 cut(s) 1937
SinI GGWCC 4 cut(s) 72, 200, 901, 993
SlaI CTCGAG 2 cut(s) 887, 1900
SmaI CCCGGG 2 cut(s) 667, 1683
SmiMI CAYNNNNRTG 1 cut(s) 1313
SmlI CTYRAG 4 cut(s) 389, 887, 1622, 1900
SmoI CTYRAG 4 cut(s) 389, 887, 1622, 1900
SrfI GCCCGGGC 1 cut(s) 667
Sse9I AATT 8 cut(s) 188, 542, 594, 605, 1108, 1628, 1945, 1983
SspDI GGCGCC 2 cut(s) 410, 914
SspMI CTAG 5 cut(s) 362, 977, 1073, 1638, 1844
SstI GAGCTC 1 cut(s) 1437
StyI CCWWGG 1 cut(s) 1224
TaaI ACNGT 4 cut(s) 200, 300, 951, 1095
TaiI ACGT 6 cut(s) 810, 1161, 1199, 1548, 1695, 1874
TaqII GACCGA 2 cut(s) 395, 1589
TasI AATT 8 cut(s) 188, 542, 594, 605, 1108, 1628, 1945, 1983
TatI WGTACW 4 cut(s) 810, 1185, 1650, 1923
TauI GCSGC 8 cut(s) 411, 618, 882, 1215, 1374, 1377, 1843, 2014
TfiI GAWTC 4 cut(s) 1500, 1562, 1742, 1787
Tru1I TTAA 5 cut(s) 191, 390, 1124, 1794, 1917
Tru9I TTAA 5 cut(s) 191, 390, 1124, 1794, 1917
TscAI CASTG 6 cut(s) 90, 139, 1100, 1761, 1772, 2021
TseFI GTSAC 3 cut(s) 1165, 1492, 1658
TseI GCWGC 1 cut(s) 1770
Tsp45I GTSAC 3 cut(s) 1165, 1492, 1658
TspDTI ATGAA 4 cut(s) 170, 292, 665, 1072
TspGWI ACGGA 1 cut(s) 929
TspMI CCCGGG 2 cut(s) 665, 1681
TspRI CASTG 6 cut(s) 90, 139, 1100, 1761, 1772, 2021
Tth111I GACNNNGTC 2 cut(s) 332, 1403
Vha464I CTTAAG 1 cut(s) 389
VpaK11BI GGWCC 4 cut(s) 72, 200, 901, 993
VspI ATTAAT 1 cut(s) 1917
XagI CCTNNNNNAGG 1 cut(s) 1406
XapI RAATTY 3 cut(s) 542, 1628, 1945
XceI RCATGY 2 cut(s) 862, 1233
XhoI CTCGAG 2 cut(s) 887, 1900
XmaI CCCGGG 2 cut(s) 665, 1681
XmiI GTMKAC 2 cut(s) 240, 1602
XspI CTAG 5 cut(s) 362, 977, 1073, 1638, 1844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.