MD05G1092900.v1.1

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013876
Physical Location & Seq
Forward (+)
19753185 .. 19755519
2335 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1092900.v1.1.491

Sequence Viewer

Length: 1806 bp
ATGGCTGTCGACTCGTCACTCTCTCCCCTCGGCCCACCCGCCTGCGAGAAGGACGCCAAAGCGTTACAGTTCATTGAAGAAACTACCCGGAACGCCGACGCCGTGCAAGAGAGGGTGCTGTCCGAGATACTAGCTCGAAACGCCCAGACCGAGTACCTTAAACGCTTCAAACTCGGCGGCGCCACCGACCGCAAAACTTTCAAGTCCAAACTCCCGGTGATTACCTACGAGGATCTCCAGCCCGAAATCCAACGCATCGCCAATGGTGATCGCTCCCCCATCTTGTCAGCTCATCCCATCTCAGAATTCCTCACCAGTTCTGGGACTTCAGCTGGAGAGAGGAAGCTGATGCCAACAATTCAGGAGGAATTGGATCGTCGCCAATTATTATACAGCCTTCTTATGCCCGTCATGAACCTTTGTGTGCCCGGGCTGGACAAGGGAAAGGGCCTTTACTTTCTGTTCGTAAAGTCCGAAACAAAGACGCCCGGCGGGCTTTTGGCCCGACCCGTTCTCACCAGCTACTACAAAAGCGAGCACTTCAAGACGCGCCCGTACGACCCGTACAACGTGTACACGAGTCCCACTGATGCAATCCTTTGCCCGGACTCCTTCCAGAGCATGTACGCCCAAATGCTCTGCGGCCTTCTCGAGCGCGAAAAAGTCCTCCGCCTCGGCGCCGTTTTCGCGTCCGGTCTCCTCCGCGCAATACGGTTCCTCCAGCTTAACTGGCAACAGCTGGCGGAAGACATTCGGACCGGGACTCTCAGCTCTAAAATAACTGACTCGGATTTGAAATCCTGCATAGGCAGGATCTTAAAACCCGACCCGGAGCTGGCGGATTTCATGTCCAAACAGTGCGGAAACGAAAATTGGGATGGGATTTTGACCCGGATATGGCCAAACACGAAATATCTAGATGTGATAGTGACGGGCGCGATGGCGCAGTACATTCCGACGTTGGATTACTACAGCGGCGGATTGCCGATGGCATGTACGATGTACGCATCGTCGGAGTGTTATTTCGGACTCAACCTCAACCCGATGTGCAAGCCTTCTGAAGTTTCCTACACGATAATGCCAAACATGGCCTATTTCGAGTTCCTTCCCTACGAGCCAAACTTAGTGAGTGGCGGTGGCGAGTCGGGAACCAGACTCGTTGACCTGGTCAACGTCGAGGTCGGAAGAGAGTACGAGCTGGTGATCACCACCTATGCCGGGCTTCACCGGTACAGAGTTGGCGACATCCTCCGAGTCACCGGGTTCCACAACTCGGCCCCGCAGTTCCACTTCGTGAGGAGGAAGAACGTTTTGCTCAGTATTGACTCGGACAAGACCGACGAGGCAGAGTTGCAACTCGCCATCGACAACGCCTCCAAGCGCCTCCAGAAATTCAGCGCTAGCGTGGTGGAGTACACAAGCTACGCCGACACGACGACAATCCCGGGGCACTATGTTATCTACTGGGAACTTCTCGTGAAGGACTCGGCGAACTCGGAGGGCGAGTCGTTTGACTCGACGATGGACCAATGCTGCCTGGCAATGGAGGAATCGCTTAACTCGGTGTACCGGCAAGGTCGAGTGTCGGACAACTCGATCGGGCCGCTGGAGATACGCGTGGTGAAGAGTGGCACATTCGAGGAGTTAATGGACTACGCAATCTCGAGAGGCGCGTCGATTAACCAGTACAAGGTGCCGCGATGTGTGAATTTTACCCCAATCATGGAGTTACTAGACTCCAGGGTAATTTCCAAGCATTTCAGCCCATCTTTACCTCACTGGACTCCTGAAAGGAAGATGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GH3

Protein Analysis

602

Amino Acids

67.56

Weight (kDa)

5.8

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH3 PF03321 22 - 330 4.9e-121 GH3 family N-terminal domain
GH3_M PF23571 357 - 434 1.1e-28 GH3 family central domain
GH3_C PF23572 450 - 571 1.5e-39 GH3 family C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013876)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14960
fragaria_vesca FvH4_2g04750
malus_domestica MD05G1092300.v1.1 MD05G1092900.v1.1
prunus_persica Prupe.8G137900_v2.0.a1
pyrus_communis pycom05g09190 pycom05g09210
rosa_chinensis RchiOBHm_Chr6g0254401
rosa_laevigata RLG00000014915
rosa_multiflora Rmu_ssc0000481.1_g000001
rosa_roxburghii Rroxscaffold_7G00210830
rosa_rugosa Rorug05G0552100
rosa_samantha Rh6AG069100 Rh6BG061700 Rh6CG061900 Rh6DG058900
rosa_wichuraiana Rw6G006070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 179, 677, 1693
AccI GTMKAC 1 cut(s) 9
AccII CGCG 8 cut(s) 550, 657, 689, 705, 938, 1617, 1673, 1699
AclI AACGTT 1 cut(s) 1308
AclWI GGATC 3 cut(s) 240, 381, 821
AcoI YGGCCR 1 cut(s) 899
AcsI RAATTY 3 cut(s) 305, 1391, 1708
AcuI CTGAAG 2 cut(s) 312, 1080
AcyI GRCGYC 5 cut(s) 54, 99, 180, 485, 678
AdeI CACNNNGTG 1 cut(s) 1294
AfeI AGCGCT 1 cut(s) 1399
AfiI CCNNNNNNNGG 9 cut(s) 321, 604, 835, 897, 1218, 1273, 1543, 1690, 1723
AflIII ACRYGT 2 cut(s) 570, 1615
AgeI ACCGGT 1 cut(s) 1227
AgsI TTSAA 5 cut(s) 77, 169, 202, 544, 796
AjnI CCWGG 3 cut(s) 1164, 1536, 1739
AjuI GAANNNNNNNTTGG 2 cut(s) 856, 888
Alw21I GWGCWC 1 cut(s) 540
Alw26I GTCTC 1 cut(s) 701
AlwI GGATC 3 cut(s) 240, 381, 821
Ama87I CYCGRG 4 cut(s) 428, 650, 1444, 1663
Aor51HI AGCGCT 1 cut(s) 1399
AoxI GGCC 8 cut(s) 31, 448, 501, 643, 899, 1089, 1275, 1601
ApeKI GCWGC 1 cut(s) 1533
ApoI RAATTY 3 cut(s) 305, 1391, 1708
AsiGI ACCGGT 1 cut(s) 1227
Asp700I GAANNNNTTC 1 cut(s) 750
AspS9I GGNCC 7 cut(s) 32, 448, 502, 756, 1276, 1525, 1601
AsuHPI GGTGA 9 cut(s) 229, 278, 304, 508, 1198, 1213, 1217, 1249, 1633
AsuNHI GCTAGC 1 cut(s) 1400
AvaI CYCGRG 4 cut(s) 428, 650, 1444, 1663
AvaII GGWCC 2 cut(s) 756, 1525
BaeGI GKGCMC 2 cut(s) 429, 1452
BalI TGGCCA 1 cut(s) 901
BanI GGYRCC 3 cut(s) 179, 677, 1693
BauI CACGAG 2 cut(s) 577, 1475
BbsI GAAGAC 1 cut(s) 753
Bbv12I GWGCWC 1 cut(s) 540
BbvI GCAGC 1 cut(s) 1520
BccI CCATC 8 cut(s) 287, 305, 872, 934, 982, 1370, 1516, 1774
BceAI ACGGC 2 cut(s) 86, 665
BciT130I CCWGG 3 cut(s) 1166, 1538, 1741
BclI TGATCA 1 cut(s) 1203
BcoDI GTCTC 1 cut(s) 701
BfaI CTAG 4 cut(s) 131, 917, 1401, 1733
BfmI CTRYAG 1 cut(s) 970
BfoI RGCGCY 4 cut(s) 183, 681, 1384, 1401
BglI GCCNNNNNGGC 1 cut(s) 493
BisI GCNGC 6 cut(s) 178, 643, 976, 1534, 1604, 1697
BlsI GCNGC 6 cut(s) 179, 644, 977, 1535, 1605, 1698
Bme18I GGWCC 2 cut(s) 756, 1525
BmeT110I CYCGRG 4 cut(s) 428, 650, 1444, 1663
BmgT120I GGNCC 7 cut(s) 32, 448, 502, 756, 1276, 1525, 1601
BmiI GGNNCC 7 cut(s) 181, 679, 716, 1150, 1265, 1278, 1695
BmrI ACTGGG 1 cut(s) 1474
BmsI GCATC 5 cut(s) 264, 339, 580, 1016, 1788
BmtI GCTAGC 1 cut(s) 1404
BmuI ACTGGG 1 cut(s) 1474
BpiI GAAGAC 1 cut(s) 753
BpmI CTGGAG 6 cut(s) 221, 354, 704, 1370, 1628, 1723
BsaHI GRCGYC 5 cut(s) 54, 99, 180, 485, 678
BsaI GGTCTC 1 cut(s) 701
BsaJI CCNNGG 6 cut(s) 28, 428, 673, 1444, 1445, 1740
BsaWI WCCGGW 2 cut(s) 692, 1227
BsaXI ACNNNNNCTCC 6 cut(s) 702, 732, 1358, 1388, 1490, 1520
Bsc4I CCNNNNNNNGG 9 cut(s) 321, 604, 835, 897, 1218, 1273, 1543, 1690, 1723
Bse118I RCCGGY 2 cut(s) 1227, 1569
Bse1I ACTGG 5 cut(s) 315, 734, 1469, 1684, 1784
Bse3DI GCAATG 1 cut(s) 1548
BseBI CCWGG 3 cut(s) 1166, 1538, 1741
BseDI CCNNGG 6 cut(s) 28, 428, 673, 1444, 1445, 1740
BseGI GGATG 3 cut(s) 292, 883, 1245
BseLI CCNNNNNNNGG 9 cut(s) 321, 604, 835, 897, 1218, 1273, 1543, 1690, 1723
BseMI GCAATG 1 cut(s) 1548
BseMII CTCAG 3 cut(s) 315, 781, 1330
BseNI ACTGG 5 cut(s) 315, 734, 1469, 1684, 1784
BseRI GAGGAG 3 cut(s) 689, 1312, 1655
BseSI GKGCMC 2 cut(s) 429, 1452
BseXI GCAGC 1 cut(s) 1520
Bsh1236I CGCG 8 cut(s) 550, 657, 689, 705, 938, 1617, 1673, 1699
Bsh1285I CGRYCG 2 cut(s) 190, 1599
BshFI GGCC 8 cut(s) 33, 450, 503, 645, 901, 1091, 1277, 1603
BshNI GGYRCC 3 cut(s) 179, 677, 1693
BshTI ACCGGT 1 cut(s) 1227
BsiEI CGRYCG 2 cut(s) 190, 1599
BsiHKAI GWGCWC 1 cut(s) 540
BsiHKCI CYCGRG 4 cut(s) 428, 650, 1444, 1663
BsiWI CGTACG 1 cut(s) 555
BslFI GGGAC 3 cut(s) 337, 567, 775
BslI CCNNNNNNNGG 9 cut(s) 321, 604, 835, 897, 1218, 1273, 1543, 1690, 1723
BsmAI GTCTC 1 cut(s) 701
BsmFI GGGAC 3 cut(s) 337, 567, 775
BsnI GGCC 8 cut(s) 33, 450, 503, 645, 901, 1091, 1277, 1603
Bso31I GGTCTC 1 cut(s) 701
BsoBI CYCGRG 4 cut(s) 428, 650, 1444, 1663
Bsp1286I GDGCHC 3 cut(s) 429, 540, 1452
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 6 cut(s) 232, 268, 373, 813, 1203, 1596
BspANI GGCC 8 cut(s) 33, 450, 503, 645, 901, 1091, 1277, 1603
BspCNI CTCAG 3 cut(s) 314, 780, 1329
BspFNI CGCG 8 cut(s) 550, 657, 689, 705, 938, 1617, 1673, 1699
BspHI TCATGA 1 cut(s) 411
BspLI GGNNCC 7 cut(s) 181, 679, 716, 1150, 1265, 1278, 1695
BspOI GCTAGC 1 cut(s) 1404
BspPI GGATC 3 cut(s) 240, 381, 821
BspT107I GGYRCC 3 cut(s) 179, 677, 1693
BspTNI GGTCTC 1 cut(s) 701
BsrDI GCAATG 1 cut(s) 1548
BsrFI RCCGGY 2 cut(s) 1227, 1569
BsrGI TGTACA 1 cut(s) 573
BsrI ACTGG 5 cut(s) 315, 734, 1469, 1684, 1784
BssAI RCCGGY 2 cut(s) 1227, 1569
BssECI CCNNGG 6 cut(s) 28, 428, 673, 1444, 1445, 1740
BssMI GATC 6 cut(s) 232, 268, 373, 813, 1203, 1596
BssNI GRCGYC 5 cut(s) 54, 99, 180, 485, 678
BssSI CACGAG 2 cut(s) 577, 1475
Bst2BI CACGAG 2 cut(s) 577, 1475
Bst2UI CCWGG 3 cut(s) 1166, 1538, 1741
Bst4CI ACNGT 3 cut(s) 69, 714, 858
Bst6I CTCTTC 2 cut(s) 1180, 1619
BstACI GRCGYC 5 cut(s) 54, 99, 180, 485, 678
BstAUI TGTACA 1 cut(s) 573
BstC8I GCNNGC 7 cut(s) 43, 494, 536, 741, 837, 1052, 1402
BstDEI CTNAG 4 cut(s) 301, 767, 1123, 1316
BstF5I GGATG 3 cut(s) 292, 883, 1245
BstFNI CGCG 8 cut(s) 550, 657, 689, 705, 938, 1617, 1673, 1699
BstH2I RGCGCY 4 cut(s) 183, 681, 1384, 1401
BstKTI GATC 6 cut(s) 235, 271, 376, 816, 1206, 1599
BstMAI GTCTC 1 cut(s) 701
BstMBI GATC 6 cut(s) 232, 268, 373, 813, 1203, 1596
BstMCI CGRYCG 2 cut(s) 190, 1599
BstMWI GCNNNNNNNGC 4 cut(s) 140, 493, 686, 730
BstNI CCWGG 3 cut(s) 1166, 1538, 1741
BstNSI RCATGY 2 cut(s) 625, 996
BstSFI CTRYAG 1 cut(s) 970
BstSLI GKGCMC 2 cut(s) 429, 1452
BstUI CGCG 8 cut(s) 550, 657, 689, 705, 938, 1617, 1673, 1699
BstV1I GCAGC 1 cut(s) 1520
BstV2I GAAGAC 1 cut(s) 753
BstX2I RGATCY 2 cut(s) 232, 813
BstYI RGATCY 2 cut(s) 232, 813
BsuRI GGCC 8 cut(s) 33, 450, 503, 645, 901, 1091, 1277, 1603
BtgZI GCGATG 3 cut(s) 241, 953, 1714
BtsCI GGATG 3 cut(s) 292, 883, 1245
BtsIMutI CAGTG 3 cut(s) 585, 863, 1777
Cac8I GCNNGC 7 cut(s) 43, 494, 536, 741, 837, 1052, 1402
CciI TCATGA 1 cut(s) 411
Cfr10I RCCGGY 2 cut(s) 1227, 1569
Cfr13I GGNCC 7 cut(s) 32, 448, 502, 756, 1276, 1525, 1601
Cfr9I CCCGGG 2 cut(s) 428, 1444
CpoI CGGWCCG 1 cut(s) 756
CseI GACGC 6 cut(s) 62, 107, 493, 556, 678, 1662
CsiI ACCWGGT 1 cut(s) 1164
CspAI ACCGGT 1 cut(s) 1227
CspI CGGWCCG 1 cut(s) 756
CviAII CATG 6 cut(s) 412, 622, 847, 993, 1087, 1723
DdeI CTNAG 4 cut(s) 301, 767, 1123, 1316
DinI GGCGCC 2 cut(s) 181, 679
DpnI GATC 6 cut(s) 234, 270, 375, 815, 1205, 1598
DpnII GATC 6 cut(s) 232, 268, 373, 813, 1203, 1596
DraIII CACNNNGTG 1 cut(s) 1294
EaeI YGGCCR 1 cut(s) 899
Eam1104I CTCTTC 2 cut(s) 1180, 1619
EarI CTCTTC 2 cut(s) 1180, 1619
EciI GGCGGA 4 cut(s) 659, 758, 854, 993
Eco31I GGTCTC 1 cut(s) 701
Eco47I GGWCC 2 cut(s) 756, 1525
Eco47III AGCGCT 1 cut(s) 1399
Eco57I CTGAAG 2 cut(s) 312, 1080
Eco88I CYCGRG 4 cut(s) 428, 650, 1444, 1663
EcoO109I RGGNCCY 1 cut(s) 448
EcoRI GAATTC 1 cut(s) 305
EcoRII CCWGG 3 cut(s) 1164, 1536, 1739
EgeI GGCGCC 2 cut(s) 181, 679
EheI GGCGCC 2 cut(s) 181, 679
FaeI CATG 6 cut(s) 415, 625, 850, 996, 1090, 1726
FaqI GGGAC 3 cut(s) 337, 567, 775
FatI CATG 6 cut(s) 411, 621, 846, 992, 1086, 1722
FauI CCCGC 3 cut(s) 46, 485, 1287
FbaI TGATCA 1 cut(s) 1203
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 6 cut(s) 178, 643, 976, 1534, 1604, 1697
FokI GGATG 3 cut(s) 279, 890, 1232
Fsp4HI GCNGC 6 cut(s) 178, 643, 976, 1534, 1604, 1697
FspBI CTAG 4 cut(s) 131, 917, 1401, 1733
GluI GCNGC 6 cut(s) 178, 643, 976, 1534, 1604, 1697
GsuI CTGGAG 6 cut(s) 221, 354, 704, 1370, 1628, 1723
HaeII RGCGCY 4 cut(s) 183, 681, 1384, 1401
HaeIII GGCC 8 cut(s) 33, 450, 503, 645, 901, 1091, 1277, 1603
HgaI GACGC 6 cut(s) 62, 107, 493, 556, 678, 1662
Hin1I GRCGYC 5 cut(s) 54, 99, 180, 485, 678
Hin1II CATG 6 cut(s) 415, 625, 850, 996, 1090, 1726
HincII GTYRAC 3 cut(s) 10, 1162, 1171
HindII GTYRAC 3 cut(s) 10, 1162, 1171
HphI GGTGA 9 cut(s) 229, 278, 304, 508, 1198, 1213, 1217, 1249, 1633
Hpy166II GTNNAC 7 cut(s) 10, 574, 576, 1162, 1171, 1416, 1567
Hpy8I GTNNAC 7 cut(s) 10, 574, 576, 1162, 1171, 1416, 1567
Hpy99I CGWCG 9 cut(s) 101, 381, 961, 1015, 1178, 1343, 1438, 1522, 1678
HpyAV CCTTC 7 cut(s) 43, 407, 622, 656, 1065, 1115, 1474
HpyCH4III ACNGT 3 cut(s) 69, 714, 858
HpyCH4IV ACGT 4 cut(s) 570, 959, 1173, 1308
HpyCH4V TGCA 5 cut(s) 106, 593, 804, 1050, 1354
HpyF10VI GCNNNNNNNGC 4 cut(s) 140, 493, 686, 730
HpyF3I CTNAG 4 cut(s) 301, 767, 1123, 1316
HpySE526I ACGT 4 cut(s) 570, 959, 1173, 1308
Hsp92I GRCGYC 5 cut(s) 54, 99, 180, 485, 678
Hsp92II CATG 6 cut(s) 415, 625, 850, 996, 1090, 1726
KasI GGCGCC 2 cut(s) 179, 677
Ksp22I TGATCA 1 cut(s) 1203
Kzo9I GATC 6 cut(s) 232, 268, 373, 813, 1203, 1596
LmnI GCTCC 2 cut(s) 278, 832
Lsp1109I GCAGC 1 cut(s) 1520
LweI GCATC 5 cut(s) 264, 339, 580, 1016, 1788
MabI ACCWGGT 1 cut(s) 1164
MaeI CTAG 4 cut(s) 131, 917, 1401, 1733
MaeII ACGT 4 cut(s) 570, 959, 1173, 1308
MaeIII GTNAC 5 cut(s) 15, 63, 928, 1255, 1728
MalI GATC 6 cut(s) 234, 270, 375, 815, 1205, 1598
MboI GATC 6 cut(s) 232, 268, 373, 813, 1203, 1596
MboII GAAGA 5 cut(s) 89, 758, 1197, 1315, 1636
MflI RGATCY 2 cut(s) 232, 813
MhlI GDGCHC 3 cut(s) 429, 540, 1452
MlsI TGGCCA 1 cut(s) 901
MluCI AATT 8 cut(s) 305, 357, 368, 383, 871, 1391, 1708, 1746
MluI ACGCGT 1 cut(s) 1615
MluNI TGGCCA 1 cut(s) 901
Mly113I GGCGCC 2 cut(s) 180, 678
MmeI TCCRAC 6 cut(s) 274, 942, 980, 993, 1162, 1566
Mox20I TGGCCA 1 cut(s) 901
MroXI GAANNNNTTC 1 cut(s) 750
MscI TGGCCA 1 cut(s) 901
MseI TTAA 6 cut(s) 159, 726, 818, 1557, 1646, 1680
MslI CAYNNNNRTG 1 cut(s) 1076
Msp20I TGGCCA 1 cut(s) 901
MspA1I CMGCKG 4 cut(s) 332, 739, 975, 1606
MvaI CCWGG 3 cut(s) 1166, 1538, 1741
MvnI CGCG 8 cut(s) 550, 657, 689, 705, 938, 1617, 1673, 1699
MwoI GCNNNNNNNGC 4 cut(s) 140, 493, 686, 730
NarI GGCGCC 2 cut(s) 180, 678
NdeII GATC 6 cut(s) 232, 268, 373, 813, 1203, 1596
NheI GCTAGC 1 cut(s) 1400
NlaIII CATG 6 cut(s) 415, 625, 850, 996, 1090, 1726
NlaIV GGNNCC 7 cut(s) 181, 679, 716, 1150, 1265, 1278, 1695
NmeAIII GCCGAG 5 cut(s) 9, 153, 654, 1253, 1466
NmuCI GTSAC 3 cut(s) 15, 928, 1255
NspI RCATGY 2 cut(s) 625, 996
PaeR7I CTCGAG 2 cut(s) 650, 1663
PagI TCATGA 1 cut(s) 411
PcsI WCGNNNNNNNCGW 6 cut(s) 99, 147, 471, 1514, 1592, 1670
PdmI GAANNNNTTC 1 cut(s) 750
PfeI GAWTC 1 cut(s) 1550
Pfl23II CGTACG 1 cut(s) 555
PflFI GACNNNGTC 1 cut(s) 1166
PinAI ACCGGT 1 cut(s) 1227
PkrI GCNGC 6 cut(s) 179, 644, 977, 1535, 1605, 1698
Ple19I CGATCG 1 cut(s) 1599
PluTI GGCGCC 2 cut(s) 183, 681
Psp1406I AACGTT 1 cut(s) 1308
Psp6I CCWGG 3 cut(s) 1164, 1536, 1739
PspGI CCWGG 3 cut(s) 1164, 1536, 1739
PspLI CGTACG 1 cut(s) 555
PspN4I GGNNCC 7 cut(s) 181, 679, 716, 1150, 1265, 1278, 1695
PspPI GGNCC 7 cut(s) 32, 448, 502, 756, 1276, 1525, 1601
PsuI RGATCY 2 cut(s) 232, 813
PsyI GACNNNGTC 1 cut(s) 1166
PvuI CGATCG 1 cut(s) 1599
PvuII CAGCTG 2 cut(s) 332, 739
RseI CAYNNNNRTG 1 cut(s) 1076
Rsr2I CGGWCCG 1 cut(s) 756
RsrII CGGWCCG 1 cut(s) 756
SalI GTCGAC 1 cut(s) 8
SaqAI TTAA 6 cut(s) 159, 726, 818, 1557, 1646, 1680
SatI GCNGC 6 cut(s) 178, 643, 976, 1534, 1604, 1697
Sau3AI GATC 6 cut(s) 232, 268, 373, 813, 1203, 1596
Sau96I GGNCC 7 cut(s) 32, 448, 502, 756, 1276, 1525, 1601
SduI GDGCHC 3 cut(s) 429, 540, 1452
SexAI ACCWGGT 1 cut(s) 1164
SfaNI GCATC 5 cut(s) 264, 339, 580, 1016, 1788
SfcI CTRYAG 1 cut(s) 970
SfoI GGCGCC 2 cut(s) 181, 679
Sfr274I CTCGAG 2 cut(s) 650, 1663
SinI GGWCC 2 cut(s) 756, 1525
SlaI CTCGAG 2 cut(s) 650, 1663
SmaI CCCGGG 2 cut(s) 430, 1446
SmiMI CAYNNNNRTG 1 cut(s) 1076
SmlI CTYRAG 2 cut(s) 650, 1663
SmoI CTYRAG 2 cut(s) 650, 1663
SrfI GCCCGGGC 1 cut(s) 430
Sse9I AATT 8 cut(s) 305, 357, 368, 383, 871, 1391, 1708, 1746
SspDI GGCGCC 2 cut(s) 179, 677
SspMI CTAG 4 cut(s) 131, 917, 1401, 1733
TaaI ACNGT 3 cut(s) 69, 714, 858
TaiI ACGT 4 cut(s) 573, 962, 1176, 1311
TaqII GACCGA 2 cut(s) 164, 1352
TasI AATT 8 cut(s) 305, 357, 368, 383, 871, 1391, 1708, 1746
TatI WGTACW 4 cut(s) 573, 948, 1413, 1686
TauI GCSGC 5 cut(s) 180, 645, 978, 1606, 1699
TfiI GAWTC 1 cut(s) 1550
Tru1I TTAA 6 cut(s) 159, 726, 818, 1557, 1646, 1680
Tru9I TTAA 6 cut(s) 159, 726, 818, 1557, 1646, 1680
TscAI CASTG 3 cut(s) 592, 863, 1784
TseFI GTSAC 3 cut(s) 15, 928, 1255
TseI GCWGC 1 cut(s) 1533
Tsp45I GTSAC 3 cut(s) 15, 928, 1255
TspDTI ATGAA 3 cut(s) 61, 428, 835
TspMI CCCGGG 2 cut(s) 428, 1444
TspRI CASTG 3 cut(s) 592, 863, 1784
Tth111I GACNNNGTC 1 cut(s) 1166
VpaK11BI GGWCC 2 cut(s) 756, 1525
XapI RAATTY 3 cut(s) 305, 1391, 1708
XbaI TCTAGA 1 cut(s) 916
XceI RCATGY 2 cut(s) 625, 996
XhoI CTCGAG 2 cut(s) 650, 1663
XmaI CCCGGG 2 cut(s) 428, 1444
XmiI GTMKAC 1 cut(s) 9
XmnI GAANNNNTTC 1 cut(s) 750
XspI CTAG 4 cut(s) 131, 917, 1401, 1733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.