FvH4_2g05230

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009594
Physical Location & Seq
Forward (+)
4322444 .. 4326235
3792 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05230.t1

Sequence Viewer

Length: 624 bp
ATGGGAGATTCCATGCCTCCGCATCGCATTTCCGTCCATCAAGCTCTCGGCGGCGGCGCCGTTGCTGATGTGCTGCTGTGGAGGCAATGGGGTGCAACAGTCGTAGTGCTGACCTCATCAACAGCGGCGTGGATCCTATTCGAGCGAGCTGGTTACAATCTCTTGTCCTTTGGCGCCAATGTTCTACTGCTTCTTGTGGTTATTCTCTTCTTCTGGGCCAAATCTGCTTCACTTCTCAATAGACCATTGCCTCCTCTTCCTGATATGGAAATATCCGAGGCATCTGTCGTGAAGGTTGCTGATGCAATGCATTTTTGGATTAATCGTGGACTGTCTGTTGCGCGAGACATTGCTATTGGCAGGAATTTGAAGCTCTTTCTTCAGGTTTCTGTTTTGTTGTGGGTAGCATCTTACATCGGAAGTCTCTTCAACTTCCTCACTCTAGCCTATGTTGGGGTTCTAATCAGTCTTTCAGTGCCCGTGTTGTATGATAAGTTTCAGCACCACATTGATGATAAGCTGTCCATAGCACATAGAACAATACAAACACAGTACAGGAAACTTGATGAGAGCATCCTAAGGAAGTTACCTATGCAATCAAACAAGCAGAAGAAGGTCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

208

Amino Acids

23.13

Weight (kDa)

9.82

Isoelectric Point (pI)

31.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 21 - 175 1.8e-52 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 56, 173
AccII CGCG 1 cut(s) 343
AciI CCGC 4 cut(s) 20, 51, 54, 125
AclWI GGATC 2 cut(s) 127, 140
AcsI RAATTY 1 cut(s) 364
AcuI CTGAAG 1 cut(s) 365
AcyI GRCGYC 2 cut(s) 57, 174
AfaI GTAC 1 cut(s) 554
AfiI CCNNNNNNNGG 1 cut(s) 453
AgsI TTSAA 2 cut(s) 370, 430
AluBI AGCT 4 cut(s) 44, 149, 373, 520
AluI AGCT 4 cut(s) 44, 149, 373, 520
Alw26I GTCTC 2 cut(s) 339, 428
AlwI GGATC 2 cut(s) 127, 140
AoxI GGCC 1 cut(s) 216
ApeKI GCWGC 1 cut(s) 73
ApoI RAATTY 1 cut(s) 364
AseI ATTAAT 1 cut(s) 321
AspLEI GCGC 3 cut(s) 59, 176, 343
AspS9I GGNCC 2 cut(s) 216, 616
AvaII GGWCC 1 cut(s) 616
AxyI CCTNAGG 1 cut(s) 578
BaeGI GKGCMC 1 cut(s) 480
BamHI GGATCC 1 cut(s) 132
BanI GGYRCC 2 cut(s) 56, 173
BbvI GCAGC 1 cut(s) 60
BccI CCATC 1 cut(s) 45
BceAI ACGGC 1 cut(s) 44
BcoDI GTCTC 2 cut(s) 339, 428
BfaI CTAG 1 cut(s) 443
BfoI RGCGCY 2 cut(s) 60, 177
BisI GCNGC 4 cut(s) 52, 55, 74, 126
BlsI GCNGC 4 cut(s) 53, 56, 75, 127
Bme18I GGWCC 1 cut(s) 616
BmgT120I GGNCC 2 cut(s) 216, 616
BmiI GGNNCC 3 cut(s) 58, 134, 175
BmsI GCATC 5 cut(s) 31, 290, 292, 416, 582
BsaHI GRCGYC 2 cut(s) 57, 174
BsaJI CCNNGG 1 cut(s) 276
Bsc4I CCNNNNNNNGG 1 cut(s) 453
Bse1I ACTGG 1 cut(s) 619
Bse21I CCTNAGG 1 cut(s) 578
Bse3DI GCAATG 4 cut(s) 92, 245, 312, 348
BseDI CCNNGG 1 cut(s) 276
BseGI GGATG 1 cut(s) 573
BseLI CCNNNNNNNGG 1 cut(s) 453
BseMI GCAATG 4 cut(s) 92, 245, 312, 348
BseNI ACTGG 1 cut(s) 619
BseRI GAGGAG 1 cut(s) 243
BseSI GKGCMC 1 cut(s) 480
BseXI GCAGC 1 cut(s) 60
Bsh1236I CGCG 1 cut(s) 343
BshFI GGCC 1 cut(s) 218
BshNI GGYRCC 2 cut(s) 56, 173
BslI CCNNNNNNNGG 1 cut(s) 453
BsmAI GTCTC 2 cut(s) 339, 428
BsnI GGCC 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 480
Bsp143I GATC 1 cut(s) 132
BspACI CCGC 4 cut(s) 20, 51, 54, 125
BspANI GGCC 1 cut(s) 218
BspFNI CGCG 1 cut(s) 343
BspLI GGNNCC 3 cut(s) 58, 134, 175
BspPI GGATC 2 cut(s) 127, 140
BspT107I GGYRCC 2 cut(s) 56, 173
BsrDI GCAATG 4 cut(s) 92, 245, 312, 348
BsrI ACTGG 1 cut(s) 619
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 1 cut(s) 132
BssNI GRCGYC 2 cut(s) 57, 174
Bst4CI ACNGT 3 cut(s) 100, 333, 552
Bst6I CTCTTC 3 cut(s) 212, 261, 431
BstACI GRCGYC 2 cut(s) 57, 174
BstC8I GCNNGC 1 cut(s) 147
BstDEI CTNAG 1 cut(s) 578
BstF5I GGATG 1 cut(s) 573
BstFNI CGCG 1 cut(s) 343
BstH2I RGCGCY 2 cut(s) 60, 177
BstHHI GCGC 3 cut(s) 59, 176, 343
BstKTI GATC 1 cut(s) 135
BstMAI GTCTC 2 cut(s) 339, 428
BstMBI GATC 1 cut(s) 132
BstMWI GCNNNNNNNGC 2 cut(s) 82, 224
BstSLI GKGCMC 1 cut(s) 480
BstUI CGCG 1 cut(s) 343
BstV1I GCAGC 1 cut(s) 60
BstX2I RGATCY 1 cut(s) 132
BstYI RGATCY 1 cut(s) 132
Bsu36I CCTNAGG 1 cut(s) 578
BsuRI GGCC 1 cut(s) 218
BtgZI GCGATG 1 cut(s) 8
BtsCI GGATG 1 cut(s) 573
BtsIMutI CAGTG 1 cut(s) 480
Cac8I GCNNGC 1 cut(s) 147
CfoI GCGC 3 cut(s) 59, 176, 343
Cfr13I GGNCC 2 cut(s) 216, 616
Csp6I GTAC 1 cut(s) 553
CviAII CATG 1 cut(s) 13
CviJI RGCY 6 cut(s) 44, 149, 218, 373, 446, 520
CviKI_1 RGCY 6 cut(s) 44, 149, 218, 373, 446, 520
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 1 cut(s) 578
DinI GGCGCC 2 cut(s) 58, 175
DpnI GATC 1 cut(s) 134
DpnII GATC 1 cut(s) 132
Eam1104I CTCTTC 3 cut(s) 212, 261, 431
EarI CTCTTC 3 cut(s) 212, 261, 431
Eco47I GGWCC 1 cut(s) 616
Eco57I CTGAAG 1 cut(s) 365
Eco81I CCTNAGG 1 cut(s) 578
EcoT22I ATGCAT 1 cut(s) 312
EgeI GGCGCC 2 cut(s) 58, 175
EheI GGCGCC 2 cut(s) 58, 175
FaeI CATG 1 cut(s) 16
FaiI YATR 7 cut(s) 14, 266, 450, 489, 527, 534, 593
FatI CATG 1 cut(s) 12
Fnu4HI GCNGC 4 cut(s) 52, 55, 74, 126
FokI GGATG 1 cut(s) 560
Fsp4HI GCNGC 4 cut(s) 52, 55, 74, 126
FspBI CTAG 1 cut(s) 443
GlaI GCGC 3 cut(s) 58, 175, 342
GluI GCNGC 4 cut(s) 52, 55, 74, 126
HaeII RGCGCY 2 cut(s) 60, 177
HaeIII GGCC 1 cut(s) 218
HhaI GCGC 3 cut(s) 59, 176, 343
Hin1I GRCGYC 2 cut(s) 57, 174
Hin1II CATG 1 cut(s) 16
Hin6I GCGC 3 cut(s) 57, 174, 341
HinP1I GCGC 3 cut(s) 57, 174, 341
HinfI GANTC 1 cut(s) 8
Hpy166II GTNNAC 1 cut(s) 329
Hpy188I TCNGA 2 cut(s) 277, 419
Hpy188III TCNNGA 2 cut(s) 260, 289
Hpy8I GTNNAC 1 cut(s) 329
HpyAV CCTTC 2 cut(s) 286, 607
HpyCH4III ACNGT 3 cut(s) 100, 333, 552
HpyCH4V TGCA 4 cut(s) 95, 305, 310, 595
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 224
HpyF3I CTNAG 1 cut(s) 578
Hsp92I GRCGYC 2 cut(s) 57, 174
Hsp92II CATG 1 cut(s) 16
HspAI GCGC 3 cut(s) 57, 174, 341
KasI GGCGCC 2 cut(s) 56, 173
Kzo9I GATC 1 cut(s) 132
LpnPI CCDG 6 cut(s) 135, 199, 273, 346, 368, 541
Lsp1109I GCAGC 1 cut(s) 60
LweI GCATC 5 cut(s) 31, 290, 292, 416, 582
MaeI CTAG 1 cut(s) 443
MaeIII GTNAC 2 cut(s) 152, 585
MalI GATC 1 cut(s) 134
MboI GATC 1 cut(s) 132
MboII GAAGA 6 cut(s) 199, 202, 248, 371, 418, 622
MflI RGATCY 1 cut(s) 132
MhlI GDGCHC 1 cut(s) 480
MluCI AATT 1 cut(s) 364
Mly113I GGCGCC 2 cut(s) 57, 174
MnlI CCTC 7 cut(s) 27, 75, 124, 261, 264, 271, 446
Mph1103I ATGCAT 1 cut(s) 312
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 1 cut(s) 510
MspA1I CMGCKG 1 cut(s) 125
MvnI CGCG 1 cut(s) 343
MwoI GCNNNNNNNGC 2 cut(s) 82, 224
NarI GGCGCC 2 cut(s) 57, 174
NdeII GATC 1 cut(s) 132
NlaIII CATG 1 cut(s) 16
NlaIV GGNNCC 3 cut(s) 58, 134, 175
NmeAIII GCCGAG 1 cut(s) 27
NsiI ATGCAT 1 cut(s) 312
PfeI GAWTC 1 cut(s) 8
PkrI GCNGC 4 cut(s) 53, 56, 75, 127
PluTI GGCGCC 2 cut(s) 60, 177
PshBI ATTAAT 1 cut(s) 321
PspN4I GGNNCC 3 cut(s) 58, 134, 175
PspPI GGNCC 2 cut(s) 216, 616
PsuI RGATCY 1 cut(s) 132
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
RseI CAYNNNNRTG 1 cut(s) 510
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 4 cut(s) 52, 55, 74, 126
Sau3AI GATC 1 cut(s) 132
Sau96I GGNCC 2 cut(s) 216, 616
SduI GDGCHC 1 cut(s) 480
SetI ASST 9 cut(s) 46, 116, 151, 297, 375, 387, 522, 592, 618
SfaNI GCATC 5 cut(s) 31, 290, 292, 416, 582
SfoI GGCGCC 2 cut(s) 58, 175
SinI GGWCC 1 cut(s) 616
SmiMI CAYNNNNRTG 1 cut(s) 510
Sse9I AATT 1 cut(s) 364
SsiI CCGC 4 cut(s) 20, 51, 54, 125
SspDI GGCGCC 2 cut(s) 56, 173
SspMI CTAG 1 cut(s) 443
TaaI ACNGT 3 cut(s) 100, 333, 552
TaqI TCGA 1 cut(s) 141
TasI AATT 1 cut(s) 364
TatI WGTACW 1 cut(s) 552
TauI GCSGC 3 cut(s) 54, 57, 128
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 1 cut(s) 480
TseI GCWGC 1 cut(s) 73
TspGWI ACGGA 1 cut(s) 22
TspRI CASTG 1 cut(s) 480
VpaK11BI GGWCC 1 cut(s) 616
VspI ATTAAT 1 cut(s) 321
XapI RAATTY 1 cut(s) 364
XspI CTAG 1 cut(s) 443
Zsp2I ATGCAT 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.