MD10G1096800.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009594
Physical Location & Seq
Reverse (-)
15431878 .. 15436037
4160 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1096800.v1.1.491

Sequence Viewer

Length: 684 bp
ATGGGAGAATCTATGCCTCCTCGTCGCATTTCCGTTCACCAAACTCTCGGAGGTGGCCCTGTTGCCGATGTGCTGCTGTGGAAGAAATGGAGTGGAGGAGTTTTACTGCTGACCTCTACGACGGCGTCGTGGTTGCTGTTTGAGCGAGCTGGTTACAATCTCTTGTCATTTGTGGCCAATGTTCTACTGCTTCTTGTGGTCATCCTTTTCCTCTGGGCCAAATCTGCTTCTCTTCTCAACAGACCTTTGCCTCCTCTCCCTGATATGGAAATCTCTGAGGCCAATGTTGTGAAGGCTGCTGATGCACTGCATGCTGGGATTAATCGTGTACTGTCTATTGCACGAGACATTGCTATTGGGAGAAATTGGAAGCTCTTTCTTCAGGTTGCTTTCTGCTTATGGCTAGCTTCTTATGTCGGAAGTTTCTTCAACTTCCTTACTCTTTTCTACATTGGGGTTCTTCTAAGTCTTTCAGTGCCCGTGTTGTATGATAAGTATCAGGACCACATTGATGATAAGCTGCATGTAGCAAACAGAGTAATACAAACACAGTACAGGAAAATTGATGATAGCATCCTAAAGAAAATTCCACTGGGTTCAAACAAGGAGAAGAAGCTACAGGTATTATGGAGCTCCGGAAAGTGTGATAACAACCTTATACAAGGCTTTCAACGTACGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

228

Amino Acids

25.45

Weight (kDa)

9.64

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 21 - 175 2.3e-54 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 635
AcoI YGGCCR 1 cut(s) 174
AcsI RAATTY 1 cut(s) 585
AcuI CTGAAG 1 cut(s) 365
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 3 cut(s) 330, 554, 676
AfiI CCNNNNNNNGG 1 cut(s) 265
AgsI TTSAA 3 cut(s) 430, 600, 671
AluBI AGCT 6 cut(s) 149, 373, 407, 520, 616, 633
AluI AGCT 6 cut(s) 149, 373, 407, 520, 616, 633
Alw21I GWGCWC 1 cut(s) 635
Alw26I GTCTC 1 cut(s) 339
Aor13HI TCCGGA 1 cut(s) 635
AoxI GGCC 4 cut(s) 55, 174, 216, 279
ApeKI GCWGC 3 cut(s) 73, 296, 520
ApoI RAATTY 1 cut(s) 585
AseI ATTAAT 1 cut(s) 321
AspS9I GGNCC 3 cut(s) 56, 216, 502
AsuHPI GGTGA 1 cut(s) 29
AsuNHI GCTAGC 1 cut(s) 403
AvaII GGWCC 1 cut(s) 502
BaeGI GKGCMC 1 cut(s) 480
BalI TGGCCA 1 cut(s) 176
BanII GRGCYC 1 cut(s) 635
BauI CACGAG 1 cut(s) 342
Bbv12I GWGCWC 1 cut(s) 635
BbvI GCAGC 3 cut(s) 60, 283, 507
BceAI ACGGC 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 339
BfaI CTAG 1 cut(s) 404
BfmI CTRYAG 1 cut(s) 617
BisI GCNGC 3 cut(s) 74, 297, 521
BlsI GCNGC 3 cut(s) 75, 298, 522
Bme18I GGWCC 1 cut(s) 502
BmgT120I GGNCC 3 cut(s) 56, 216, 502
BmrI ACTGGG 1 cut(s) 602
BmsI GCATC 2 cut(s) 292, 582
BmtI GCTAGC 1 cut(s) 407
BmuI ACTGGG 1 cut(s) 602
BsaBI GATNNNNATC 1 cut(s) 495
BsaHI GRCGYC 1 cut(s) 125
BsaWI WCCGGW 1 cut(s) 635
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse1I ACTGG 1 cut(s) 597
Bse3DI GCAATG 1 cut(s) 348
Bse8I GATNNNNATC 1 cut(s) 495
BseAI TCCGGA 1 cut(s) 635
BseGI GGATG 2 cut(s) 201, 573
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 1 cut(s) 265
BseMI GCAATG 1 cut(s) 348
BseMII CTCAG 1 cut(s) 267
BseNI ACTGG 1 cut(s) 597
BseRI GAGGAG 3 cut(s) 9, 111, 243
BseSI GKGCMC 1 cut(s) 480
BseXI GCAGC 3 cut(s) 60, 283, 507
BseYI CCCAGC 1 cut(s) 314
BshFI GGCC 4 cut(s) 57, 176, 218, 281
BsiHKAI GWGCWC 1 cut(s) 635
BsiSI CCGG 1 cut(s) 636
BsiWI CGTACG 1 cut(s) 674
BslI CCNNNNNNNGG 1 cut(s) 265
BsmAI GTCTC 1 cut(s) 339
BsnI GGCC 4 cut(s) 57, 176, 218, 281
Bsp1286I GDGCHC 2 cut(s) 480, 635
Bsp13I TCCGGA 1 cut(s) 635
BspANI GGCC 4 cut(s) 57, 176, 218, 281
BspCNI CTCAG 1 cut(s) 268
BspEI TCCGGA 1 cut(s) 635
BspOI GCTAGC 1 cut(s) 407
BsrDI GCAATG 1 cut(s) 348
BsrI ACTGG 1 cut(s) 597
BssNI GRCGYC 1 cut(s) 125
BssSI CACGAG 1 cut(s) 342
Bst2BI CACGAG 1 cut(s) 342
Bst4CI ACNGT 2 cut(s) 333, 552
Bst6I CTCTTC 1 cut(s) 237
BstACI GRCGYC 1 cut(s) 125
BstAPI GCANNNNNTGC 1 cut(s) 311
BstC8I GCNNGC 3 cut(s) 147, 312, 405
BstDEI CTNAG 2 cut(s) 276, 464
BstF5I GGATG 2 cut(s) 201, 573
BstMAI GTCTC 1 cut(s) 339
BstMWI GCNNNNNNNGC 4 cut(s) 142, 224, 302, 311
BstNSI RCATGY 2 cut(s) 314, 527
BstSFI CTRYAG 1 cut(s) 617
BstSLI GKGCMC 1 cut(s) 480
BstV1I GCAGC 3 cut(s) 60, 283, 507
BsuRI GGCC 4 cut(s) 57, 176, 218, 281
BtsCI GGATG 2 cut(s) 201, 573
BtsI GCAGTG 1 cut(s) 305
BtsIMutI CAGTG 3 cut(s) 305, 480, 590
Cac8I GCNNGC 3 cut(s) 147, 312, 405
Cfr13I GGNCC 3 cut(s) 56, 216, 502
CseI GACGC 1 cut(s) 114
Csp6I GTAC 3 cut(s) 329, 553, 675
CviAII CATG 2 cut(s) 311, 524
CviQI GTAC 3 cut(s) 329, 553, 675
DdeI CTNAG 2 cut(s) 276, 464
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
Ecl136II GAGCTC 1 cut(s) 633
Eco24I GRGCYC 1 cut(s) 635
Eco47I GGWCC 1 cut(s) 502
Eco53kI GAGCTC 1 cut(s) 633
Eco57I CTGAAG 1 cut(s) 365
EcoICRI GAGCTC 1 cut(s) 633
EcoT38I GRGCYC 1 cut(s) 635
FaeI CATG 2 cut(s) 314, 527
FaiI YATR 9 cut(s) 14, 266, 312, 400, 414, 489, 525, 628, 659
FatI CATG 2 cut(s) 310, 523
Fnu4HI GCNGC 3 cut(s) 74, 297, 521
FokI GGATG 2 cut(s) 188, 560
FriOI GRGCYC 1 cut(s) 635
Fsp4HI GCNGC 3 cut(s) 74, 297, 521
FspBI CTAG 1 cut(s) 404
GluI GCNGC 3 cut(s) 74, 297, 521
GsaI CCCAGC 1 cut(s) 318
HaeIII GGCC 4 cut(s) 57, 176, 218, 281
HapII CCGG 1 cut(s) 636
HgaI GACGC 1 cut(s) 114
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 2 cut(s) 314, 527
HinfI GANTC 1 cut(s) 8
HpaII CCGG 1 cut(s) 636
HphI GGTGA 1 cut(s) 29
Hpy166II GTNNAC 2 cut(s) 37, 329
Hpy188I TCNGA 3 cut(s) 50, 277, 419
Hpy188III TCNNGA 2 cut(s) 500, 636
Hpy8I GTNNAC 2 cut(s) 37, 329
Hpy99I CGWCG 3 cut(s) 27, 124, 130
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 2 cut(s) 333, 552
HpyCH4IV ACGT 1 cut(s) 673
HpyCH4V TGCA 4 cut(s) 305, 310, 341, 523
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 224, 302, 311
HpyF3I CTNAG 2 cut(s) 276, 464
HpySE526I ACGT 1 cut(s) 673
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 2 cut(s) 314, 527
Kpn2I TCCGGA 1 cut(s) 635
LmnI GCTCC 2 cut(s) 630, 638
Lsp1109I GCAGC 3 cut(s) 60, 283, 507
LweI GCATC 2 cut(s) 292, 582
MaeI CTAG 1 cut(s) 404
MaeII ACGT 1 cut(s) 673
MaeIII GTNAC 1 cut(s) 152
MboII GAAGA 6 cut(s) 94, 224, 371, 418, 452, 622
MhlI GDGCHC 2 cut(s) 480, 635
MlsI TGGCCA 1 cut(s) 176
MluCI AATT 3 cut(s) 364, 561, 585
MluNI TGGCCA 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 397
MnlI CCTC 9 cut(s) 27, 30, 44, 89, 124, 221, 261, 264, 271
Mox20I TGGCCA 1 cut(s) 176
MroI TCCGGA 1 cut(s) 635
MscI TGGCCA 1 cut(s) 176
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 1 cut(s) 510
Msp20I TGGCCA 1 cut(s) 176
MspI CCGG 1 cut(s) 636
MwoI GCNNNNNNNGC 4 cut(s) 142, 224, 302, 311
NheI GCTAGC 1 cut(s) 403
NlaIII CATG 2 cut(s) 314, 527
NspI RCATGY 2 cut(s) 314, 527
PaeI GCATGC 1 cut(s) 314
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PfeI GAWTC 1 cut(s) 8
Pfl23II CGTACG 1 cut(s) 674
PflFI GACNNNGTC 1 cut(s) 124
PkrI GCNGC 3 cut(s) 75, 298, 522
PshBI ATTAAT 1 cut(s) 321
Psp124BI GAGCTC 1 cut(s) 635
PspFI CCCAGC 1 cut(s) 314
PspLI CGTACG 1 cut(s) 674
PspPI GGNCC 3 cut(s) 56, 216, 502
PsyI GACNNNGTC 1 cut(s) 124
RsaI GTAC 3 cut(s) 330, 554, 676
RsaNI GTAC 3 cut(s) 329, 553, 675
RseI CAYNNNNRTG 1 cut(s) 510
SacI GAGCTC 1 cut(s) 635
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 3 cut(s) 74, 297, 521
Sau96I GGNCC 3 cut(s) 56, 216, 502
SduI GDGCHC 2 cut(s) 480, 635
SfaNI GCATC 2 cut(s) 292, 582
SfcI CTRYAG 1 cut(s) 617
SinI GGWCC 1 cut(s) 502
SmiMI CAYNNNNRTG 1 cut(s) 510
SphI GCATGC 1 cut(s) 314
Sse9I AATT 3 cut(s) 364, 561, 585
SspMI CTAG 1 cut(s) 404
SstI GAGCTC 1 cut(s) 635
TaaI ACNGT 2 cut(s) 333, 552
TaiI ACGT 1 cut(s) 676
TasI AATT 3 cut(s) 364, 561, 585
TatI WGTACW 2 cut(s) 328, 552
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 3 cut(s) 312, 480, 597
TseI GCWGC 3 cut(s) 73, 296, 520
TspGWI ACGGA 1 cut(s) 22
TspRI CASTG 3 cut(s) 312, 480, 597
Tth111I GACNNNGTC 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 502
VspI ATTAAT 1 cut(s) 321
XapI RAATTY 1 cut(s) 585
XceI RCATGY 2 cut(s) 314, 527
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.