MD05G1088700.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009594
Physical Location & Seq
Reverse (-)
18933520 .. 18936874
3355 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1088700.v1.1.491

Sequence Viewer

Length: 651 bp
ATGGGAGAATCTATGCCTCCTCGTCGCAATTCCGTTCATCAAGCTCTCGGAGGTGGCCATGTTGCTGATGTGCTCCTGTGGAAGAAATGGAGTGGAGGAGTTTTACTGCTGACCTCTACGACGGCGTCGTGGTTGCTGTTTGAGCGAGCTGGCTACAATCTCTTGTCCTTTGTGGCTAATGTTCTACTGCTACTTGTAGTCATCCTCTTCCTCTGGGCCAAATCGGCTTCTCTTCTCAACAGACCTTTGCCTCCTCTCCCCGATATGGAAATCTCAGAGGCTAATGTAGTGAAGGCTGCTGAGGCACTGCATGCTGGGATTAATCGTGGATTGTCTATTGCACGAGACATTGCTATTGGGAGAAATTGGAAACTCTTCCTTCAGGTCGCTTTCTGCTTGTGGCTAGCTTCTTACATCGGAAGTTTCTTCAACTTCCTTACTCTTGTCTACATTGGGGTTCTCCTAAGTCTTTCAGTGCCCATGTTGTATGATAAGTATCAGGACCACATTGATGATAAGCTGCATGTAGCAAACAGAGTAATACAAGCACAGTACAGGAAAATTGATGGTAGCATCCTAAAGAAAATTCCTTTGCGGTCAAACAAGGAGAAGAAGCTACAGCCATATTTGTTATATATGCTGGTTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

217

Amino Acids

24.26

Weight (kDa)

9.64

Isoelectric Point (pI)

36.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 21 - 175 2.8e-54 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 447
AciI CCGC 1 cut(s) 595
AcoI YGGCCR 1 cut(s) 55
AcsI RAATTY 1 cut(s) 585
AcuI CTGAAG 1 cut(s) 365
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 1 cut(s) 554
AfiI CCNNNNNNNGG 1 cut(s) 265
AgsI TTSAA 1 cut(s) 430
AluBI AGCT 5 cut(s) 44, 149, 407, 520, 616
AluI AGCT 5 cut(s) 44, 149, 407, 520, 616
Alw21I GWGCWC 1 cut(s) 75
Alw26I GTCTC 1 cut(s) 339
AoxI GGCC 2 cut(s) 55, 216
ApeKI GCWGC 2 cut(s) 296, 520
ApoI RAATTY 1 cut(s) 585
AseI ATTAAT 1 cut(s) 321
Asp700I GAANNNNTTC 1 cut(s) 374
AspS9I GGNCC 2 cut(s) 216, 502
AsuNHI GCTAGC 1 cut(s) 403
AvaII GGWCC 1 cut(s) 502
BaeGI GKGCMC 1 cut(s) 480
BalI TGGCCA 1 cut(s) 57
BauI CACGAG 1 cut(s) 342
Bbv12I GWGCWC 1 cut(s) 75
BbvCI CCTCAGC 1 cut(s) 300
BbvI GCAGC 2 cut(s) 283, 507
BccI CCATC 1 cut(s) 560
BceAI ACGGC 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 339
BfaI CTAG 1 cut(s) 404
BfmI CTRYAG 1 cut(s) 617
BglI GCCNNNNNGGC 1 cut(s) 224
BisI GCNGC 2 cut(s) 297, 521
BlsI GCNGC 2 cut(s) 298, 522
Bme18I GGWCC 1 cut(s) 502
BmgT120I GGNCC 2 cut(s) 216, 502
BmsI GCATC 1 cut(s) 582
BmtI GCTAGC 1 cut(s) 407
Bpu10I CCTNAGC 1 cut(s) 300
BsaBI GATNNNNATC 1 cut(s) 495
BsaHI GRCGYC 1 cut(s) 125
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse3DI GCAATG 1 cut(s) 348
Bse8I GATNNNNATC 1 cut(s) 495
BseGI GGATG 2 cut(s) 201, 573
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 1 cut(s) 265
BseMI GCAATG 1 cut(s) 348
BseMII CTCAG 2 cut(s) 288, 291
BseRI GAGGAG 3 cut(s) 9, 111, 243
BseSI GKGCMC 1 cut(s) 480
BseXI GCAGC 2 cut(s) 283, 507
BseYI CCCAGC 1 cut(s) 314
BshFI GGCC 2 cut(s) 57, 218
BsiHKAI GWGCWC 1 cut(s) 75
BslI CCNNNNNNNGG 1 cut(s) 265
BsmAI GTCTC 1 cut(s) 339
BsnI GGCC 2 cut(s) 57, 218
Bsp1286I GDGCHC 2 cut(s) 75, 480
BspACI CCGC 1 cut(s) 595
BspANI GGCC 2 cut(s) 57, 218
BspCNI CTCAG 2 cut(s) 287, 292
BspOI GCTAGC 1 cut(s) 407
BsrDI GCAATG 1 cut(s) 348
BssNI GRCGYC 1 cut(s) 125
BssSI CACGAG 1 cut(s) 342
Bst2BI CACGAG 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 552
Bst6I CTCTTC 3 cut(s) 212, 237, 380
BstACI GRCGYC 1 cut(s) 125
BstAPI GCANNNNNTGC 1 cut(s) 311
BstC8I GCNNGC 4 cut(s) 147, 151, 312, 405
BstDEI CTNAG 3 cut(s) 274, 300, 464
BstF5I GGATG 2 cut(s) 201, 573
BstMAI GTCTC 1 cut(s) 339
BstMWI GCNNNNNNNGC 4 cut(s) 142, 224, 302, 311
BstNSI RCATGY 2 cut(s) 314, 527
BstSFI CTRYAG 1 cut(s) 617
BstSLI GKGCMC 1 cut(s) 480
BstV1I GCAGC 2 cut(s) 283, 507
BsuRI GGCC 2 cut(s) 57, 218
BtsCI GGATG 2 cut(s) 201, 573
BtsI GCAGTG 1 cut(s) 305
BtsIMutI CAGTG 2 cut(s) 305, 480
Cac8I GCNNGC 4 cut(s) 147, 151, 312, 405
Cfr13I GGNCC 2 cut(s) 216, 502
CseI GACGC 1 cut(s) 114
Csp6I GTAC 1 cut(s) 553
CviAII CATG 4 cut(s) 59, 311, 481, 524
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 3 cut(s) 274, 300, 464
EaeI YGGCCR 1 cut(s) 55
Eam1104I CTCTTC 3 cut(s) 212, 237, 380
EarI CTCTTC 3 cut(s) 212, 237, 380
Eco47I GGWCC 1 cut(s) 502
Eco57I CTGAAG 1 cut(s) 365
FaeI CATG 4 cut(s) 62, 314, 484, 527
FatI CATG 4 cut(s) 58, 310, 480, 523
FblI GTMKAC 1 cut(s) 447
Fnu4HI GCNGC 2 cut(s) 297, 521
FokI GGATG 2 cut(s) 188, 560
Fsp4HI GCNGC 2 cut(s) 297, 521
FspBI CTAG 1 cut(s) 404
GluI GCNGC 2 cut(s) 297, 521
GsaI CCCAGC 1 cut(s) 318
HaeIII GGCC 2 cut(s) 57, 218
HgaI GACGC 1 cut(s) 114
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 4 cut(s) 62, 314, 484, 527
HinfI GANTC 1 cut(s) 8
Hpy166II GTNNAC 1 cut(s) 448
Hpy188I TCNGA 3 cut(s) 50, 277, 419
Hpy188III TCNNGA 1 cut(s) 500
Hpy8I GTNNAC 1 cut(s) 448
Hpy99I CGWCG 3 cut(s) 27, 124, 130
HpyAV CCTTC 2 cut(s) 286, 389
HpyCH4III ACNGT 1 cut(s) 552
HpyCH4V TGCA 3 cut(s) 310, 341, 523
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 224, 302, 311
HpyF3I CTNAG 3 cut(s) 274, 300, 464
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 4 cut(s) 62, 314, 484, 527
LmnI GCTCC 1 cut(s) 78
LpnPI CCDG 8 cut(s) 89, 135, 199, 300, 368, 485, 541, 626
Lsp1109I GCAGC 2 cut(s) 283, 507
LweI GCATC 1 cut(s) 582
MaeI CTAG 1 cut(s) 404
MboII GAAGA 6 cut(s) 94, 199, 224, 367, 418, 622
MhlI GDGCHC 2 cut(s) 75, 480
MlsI TGGCCA 1 cut(s) 57
MluCI AATT 4 cut(s) 28, 364, 561, 585
MluNI TGGCCA 1 cut(s) 57
Mox20I TGGCCA 1 cut(s) 57
MroXI GAANNNNTTC 1 cut(s) 374
MscI TGGCCA 1 cut(s) 57
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 1 cut(s) 510
Msp20I TGGCCA 1 cut(s) 57
MwoI GCNNNNNNNGC 4 cut(s) 142, 224, 302, 311
NheI GCTAGC 1 cut(s) 403
NlaIII CATG 4 cut(s) 62, 314, 484, 527
NspI RCATGY 2 cut(s) 314, 527
PaeI GCATGC 1 cut(s) 314
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PdmI GAANNNNTTC 1 cut(s) 374
PfeI GAWTC 1 cut(s) 8
PflFI GACNNNGTC 1 cut(s) 124
PkrI GCNGC 2 cut(s) 298, 522
PshBI ATTAAT 1 cut(s) 321
PspFI CCCAGC 1 cut(s) 314
PspPI GGNCC 2 cut(s) 216, 502
PsyI GACNNNGTC 1 cut(s) 124
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
RseI CAYNNNNRTG 1 cut(s) 510
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 2 cut(s) 297, 521
Sau96I GGNCC 2 cut(s) 216, 502
SduI GDGCHC 2 cut(s) 75, 480
SetI ASST 9 cut(s) 46, 55, 116, 151, 247, 387, 409, 522, 618
SfaNI GCATC 1 cut(s) 582
SfcI CTRYAG 1 cut(s) 617
SinI GGWCC 1 cut(s) 502
SmiMI CAYNNNNRTG 1 cut(s) 510
SphI GCATGC 1 cut(s) 314
Sse9I AATT 4 cut(s) 28, 364, 561, 585
SsiI CCGC 1 cut(s) 595
SspMI CTAG 1 cut(s) 404
TaaI ACNGT 1 cut(s) 552
TasI AATT 4 cut(s) 28, 364, 561, 585
TatI WGTACW 1 cut(s) 552
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 2 cut(s) 312, 480
TseI GCWGC 2 cut(s) 296, 520
TspDTI ATGAA 1 cut(s) 26
TspGWI ACGGA 1 cut(s) 22
TspRI CASTG 2 cut(s) 312, 480
Tth111I GACNNNGTC 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 502
VspI ATTAAT 1 cut(s) 321
XapI RAATTY 1 cut(s) 585
XceI RCATGY 2 cut(s) 314, 527
XmiI GTMKAC 1 cut(s) 447
XmnI GAANNNNTTC 1 cut(s) 374
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.