FvH4_2g06620

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015532
Physical Location & Seq
Reverse (-)
5494615 .. 5495535
921 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06620.t1

Sequence Viewer

Length: 921 bp
ATGGATTCAAACAACAATGAGATAATGCATGACTTCCCACCTTTCTTCAAAGTCTTCAGAGATGGTAGCATAGAGAGGTACATGCCACTCCATGACCCTATTCCGGCGGGACTCGACCCAACAACAGGAGTCCAATCCAAAGACGTGGTGGTTTCGCCTGAAACCGGAGCAAAGGCTCGTATTTTCATTCCCAAAATCAACGGCGGAGGTCAGAAGTTGCCCCTCTTTGTCCATTACCACGGTGGAGGCTTCTGCATGGGATCACCCTTTGGTGACATTGGAAAGAAGTTCCTTTCATCTCTAGTCCTCAAAGCCAACGTCATTGTAATTTCTGTTGGCTACAGGTTAGCCCCGGAGCATCGTCTCCCAATTGCCTACGACGATTCAGCTTCCGCGCTGCACTGGATCAGTTGCCACTCTAATGGACAAGGACCTGAAGCTTGGCTCAACGAGTATGCAGATCTGAACAGGGTCTTCTTGGGCGGTGAGAGTGCTGGAGCCAACATCGCTCACTATGTTGCGGTGCAAGCTGGGGTTACGGTTCCACTAGGGTTGAACATTGTTGGCTTAGTTATGGTGCATCCCTTCTTCGGAGGTAAAGAGCGTGACGAAATGTACAAGTTTTTGAGCTCAACAAGTTCCGGGTGCGATGATGACCCGAAACTGAACCCGGCAGCGGATCCAGACTTGGTGAAAATGCCCTGTGGGAAGGTGTTGGTTTGTGTTGCGGAGAAAGATTGGCTGAGAGATAGAGGAGTGAGCTACCATGAGACTTTAGAGAATCTTAAAGGGGAAGGGTTTGCGGAGTTGTATGAGACAAAAGGGGAGGACCATTGCTTTCATATGTTCAACCCAAACCCCAACATCGACAAGGCTGGCGATTTGCTGCTAAAAATTGTTGATTTTGTAACTCAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

307

Amino Acids

33.46

Weight (kDa)

5.51

Isoelectric Point (pI)

38.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 69 - 177 1.9e-09 BD-FAE
Abhydrolase_3 PF07859 77 - 282 9.8e-43 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 395
AciI CCGC 8 cut(s) 107, 204, 393, 483, 521, 677, 728, 803
AclWI GGATC 4 cut(s) 268, 413, 674, 687
AcuI CTGAAG 2 cut(s) 40, 456
AfaI GTAC 2 cut(s) 80, 617
AfiI CCNNNNNNNGG 5 cut(s) 103, 125, 164, 590, 676
AgsI TTSAA 4 cut(s) 9, 49, 556, 850
AjiI CACGTC 1 cut(s) 145
AluBI AGCT 5 cut(s) 389, 440, 530, 630, 762
AluI AGCT 5 cut(s) 389, 440, 530, 630, 762
Alw21I GWGCWC 1 cut(s) 632
Alw26I GTCTC 3 cut(s) 368, 764, 809
AlwI GGATC 4 cut(s) 268, 413, 674, 687
ApeKI GCWGC 3 cut(s) 397, 674, 886
ArsI GACNNNNNNTTYG 2 cut(s) 303, 335
AspLEI GCGC 1 cut(s) 397
AspS9I GGNCC 2 cut(s) 431, 829
AsuC2I CCSGG 3 cut(s) 353, 643, 671
AsuHPI GGTGA 4 cut(s) 255, 284, 497, 703
AvaII GGWCC 2 cut(s) 431, 829
BamHI GGATCC 1 cut(s) 679
BanII GRGCYC 1 cut(s) 632
BbsI GAAGAC 2 cut(s) 46, 466
Bbv12I GWGCWC 1 cut(s) 632
BbvI GCAGC 3 cut(s) 384, 686, 873
BccI CCATC 1 cut(s) 56
BceAI ACGGC 1 cut(s) 217
BcnI CCSGG 3 cut(s) 353, 643, 671
BcoDI GTCTC 3 cut(s) 368, 764, 809
BfaI CTAG 2 cut(s) 302, 548
BfmI CTRYAG 1 cut(s) 340
BglII AGATCT 1 cut(s) 460
BisI GCNGC 3 cut(s) 398, 675, 887
BlsI GCNGC 3 cut(s) 399, 676, 888
Bme1390I CCNGG 3 cut(s) 353, 643, 671
Bme18I GGWCC 2 cut(s) 431, 829
BmgBI CACGTC 1 cut(s) 145
BmgT120I GGNCC 2 cut(s) 431, 829
BmiI GGNNCC 3 cut(s) 499, 543, 681
BmrFI CCNGG 3 cut(s) 353, 643, 671
BmsI GCATC 2 cut(s) 367, 589
BpiI GAAGAC 2 cut(s) 46, 466
BpmI CTGGAG 1 cut(s) 516
BpuMI CCSGG 3 cut(s) 353, 643, 671
BsaJI CCNNGG 2 cut(s) 238, 351
BsaWI WCCGGW 1 cut(s) 164
Bsc4I CCNNNNNNNGG 5 cut(s) 103, 125, 164, 590, 676
Bse1I ACTGG 1 cut(s) 407
Bse3DI GCAATG 1 cut(s) 832
BseDI CCNNGG 2 cut(s) 238, 351
BseGI GGATG 1 cut(s) 580
BseLI CCNNNNNNNGG 5 cut(s) 103, 125, 164, 590, 676
BseMI GCAATG 1 cut(s) 832
BseMII CTCAG 1 cut(s) 734
BseNI ACTGG 1 cut(s) 407
BseRI GAGGAG 1 cut(s) 768
BseXI GCAGC 3 cut(s) 384, 686, 873
BseYI CCCAGC 1 cut(s) 530
BsgI GTGCAG 1 cut(s) 383
Bsh1236I CGCG 1 cut(s) 395
BsiHKAI GWGCWC 1 cut(s) 632
BsiSI CCGG 5 cut(s) 104, 165, 353, 642, 671
BslFI GGGAC 1 cut(s) 123
BslI CCNNNNNNNGG 5 cut(s) 103, 125, 164, 590, 676
BsmAI GTCTC 3 cut(s) 368, 764, 809
BsmBI CGTCTC 1 cut(s) 368
BsmFI GGGAC 1 cut(s) 123
Bsp1286I GDGCHC 1 cut(s) 632
Bsp1407I TGTACA 1 cut(s) 615
Bsp143I GATC 4 cut(s) 260, 405, 460, 679
BspACI CCGC 8 cut(s) 107, 204, 393, 483, 521, 677, 728, 803
BspCNI CTCAG 1 cut(s) 735
BspFNI CGCG 1 cut(s) 395
BspLI GGNNCC 3 cut(s) 499, 543, 681
BspPI GGATC 4 cut(s) 268, 413, 674, 687
BsrDI GCAATG 1 cut(s) 832
BsrGI TGTACA 1 cut(s) 615
BsrI ACTGG 1 cut(s) 407
BssECI CCNNGG 2 cut(s) 238, 351
BssMI GATC 4 cut(s) 260, 405, 460, 679
Bst4CI ACNGT 2 cut(s) 242, 541
BstAUI TGTACA 1 cut(s) 615
BstC8I GCNNGC 2 cut(s) 528, 877
BstDEI CTNAG 2 cut(s) 568, 743
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 1 cut(s) 580
BstFNI CGCG 1 cut(s) 395
BstHHI GCGC 1 cut(s) 397
BstKTI GATC 4 cut(s) 263, 408, 463, 682
BstMAI GTCTC 3 cut(s) 368, 764, 809
BstMBI GATC 4 cut(s) 260, 405, 460, 679
BstMWI GCNNNNNNNGC 2 cut(s) 506, 527
BstNSI RCATGY 1 cut(s) 85
BstSCI CCNGG 3 cut(s) 351, 641, 669
BstSFI CTRYAG 1 cut(s) 340
BstUI CGCG 1 cut(s) 395
BstV1I GCAGC 3 cut(s) 384, 686, 873
BstV2I GAAGAC 2 cut(s) 46, 466
BstX2I RGATCY 2 cut(s) 460, 679
BstXI CCANNNNNNTGG 2 cut(s) 145, 422
BstYI RGATCY 2 cut(s) 460, 679
BtgI CCRYGG 1 cut(s) 238
BtgZI GCGATG 2 cut(s) 490, 663
BtrI CACGTC 1 cut(s) 145
BtsCI GGATG 1 cut(s) 580
BtsIMutI CAGTG 1 cut(s) 400
Cac8I GCNNGC 2 cut(s) 528, 877
CfoI GCGC 1 cut(s) 397
Cfr13I GGNCC 2 cut(s) 431, 829
Csp6I GTAC 2 cut(s) 79, 616
CspCI CAANNNNNGTGG 2 cut(s) 534, 569
CviAII CATG 5 cut(s) 29, 82, 92, 256, 767
CviQI GTAC 2 cut(s) 79, 616
DdeI CTNAG 2 cut(s) 568, 743
DpnI GATC 4 cut(s) 262, 407, 462, 681
DpnII GATC 4 cut(s) 260, 405, 460, 679
EciI GGCGGA 1 cut(s) 219
Ecl136II GAGCTC 1 cut(s) 630
Eco24I GRGCYC 1 cut(s) 632
Eco47I GGWCC 2 cut(s) 431, 829
Eco53kI GAGCTC 1 cut(s) 630
Eco57I CTGAAG 2 cut(s) 40, 456
EcoICRI GAGCTC 1 cut(s) 630
EcoO109I RGGNCCY 1 cut(s) 431
EcoT22I ATGCAT 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 632
Esp3I CGTCTC 1 cut(s) 368
FaeI CATG 5 cut(s) 32, 85, 95, 259, 770
FaqI GGGAC 1 cut(s) 123
FatI CATG 5 cut(s) 28, 81, 91, 255, 766
FauI CCCGC 1 cut(s) 100
FauNDI CATATG 1 cut(s) 843
Fnu4HI GCNGC 3 cut(s) 398, 675, 887
FokI GGATG 1 cut(s) 567
FriOI GRGCYC 1 cut(s) 632
Fsp4HI GCNGC 3 cut(s) 398, 675, 887
FspBI CTAG 2 cut(s) 302, 548
GlaI GCGC 1 cut(s) 396
GluI GCNGC 3 cut(s) 398, 675, 887
GsaI CCCAGC 1 cut(s) 534
GsuI CTGGAG 1 cut(s) 516
HapII CCGG 5 cut(s) 104, 165, 353, 642, 671
HhaI GCGC 1 cut(s) 397
Hin1II CATG 5 cut(s) 32, 85, 95, 259, 770
Hin6I GCGC 1 cut(s) 395
HinP1I GCGC 1 cut(s) 395
HindIII AAGCTT 1 cut(s) 438
HinfI GANTC 5 cut(s) 5, 111, 129, 383, 781
HpaII CCGG 5 cut(s) 104, 165, 353, 642, 671
HphI GGTGA 4 cut(s) 255, 284, 497, 703
Hpy188I TCNGA 4 cut(s) 59, 213, 465, 593
Hpy188III TCNNGA 1 cut(s) 683
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 3 cut(s) 595, 703, 788
HpyCH4III ACNGT 2 cut(s) 242, 541
HpyCH4IV ACGT 2 cut(s) 144, 318
HpyCH4V TGCA 6 cut(s) 28, 255, 400, 458, 526, 580
HpyF10VI GCNNNNNNNGC 2 cut(s) 506, 527
HpyF3I CTNAG 2 cut(s) 568, 743
HpySE526I ACGT 2 cut(s) 144, 318
Hsp92II CATG 5 cut(s) 32, 85, 95, 259, 770
HspAI GCGC 1 cut(s) 395
Kzo9I GATC 4 cut(s) 260, 405, 460, 679
LmnI GCTCC 3 cut(s) 167, 355, 497
Lsp1109I GCAGC 3 cut(s) 384, 686, 873
LweI GCATC 2 cut(s) 367, 589
MaeI CTAG 2 cut(s) 302, 548
MaeII ACGT 2 cut(s) 144, 318
MaeIII GTNAC 4 cut(s) 272, 535, 605, 907
MalI GATC 4 cut(s) 262, 407, 462, 681
MboI GATC 4 cut(s) 260, 405, 460, 679
MboII GAAGA 4 cut(s) 37, 46, 466, 580
MfeI CAATTG 1 cut(s) 369
MflI RGATCY 2 cut(s) 460, 679
MhlI GDGCHC 1 cut(s) 632
MluCI AATT 3 cut(s) 327, 369, 894
MlyI GAGTC 2 cut(s) 105, 138
MnlI CCTC 8 cut(s) 69, 200, 233, 239, 317, 587, 746, 820
Mph1103I ATGCAT 1 cut(s) 30
MseI TTAA 2 cut(s) 786, 919
MslI CAYNNNNRTG 1 cut(s) 420
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 5 cut(s) 104, 165, 353, 642, 671
MspR9I CCNGG 3 cut(s) 353, 643, 671
MunI CAATTG 1 cut(s) 369
MvnI CGCG 1 cut(s) 395
MwoI GCNNNNNNNGC 2 cut(s) 506, 527
NciI CCSGG 3 cut(s) 353, 643, 671
NdeI CATATG 1 cut(s) 843
NdeII GATC 4 cut(s) 260, 405, 460, 679
NlaIII CATG 5 cut(s) 32, 85, 95, 259, 770
NlaIV GGNNCC 3 cut(s) 499, 543, 681
NmuCI GTSAC 2 cut(s) 272, 605
NsiI ATGCAT 1 cut(s) 30
NspI RCATGY 1 cut(s) 85
PfeI GAWTC 3 cut(s) 5, 383, 781
PkrI GCNGC 3 cut(s) 399, 676, 888
PleI GAGTC 2 cut(s) 105, 137
PpsI GAGTC 2 cut(s) 105, 137
PpuMI RGGWCCY 1 cut(s) 431
Psp124BI GAGCTC 1 cut(s) 632
Psp5II RGGWCCY 1 cut(s) 431
PspFI CCCAGC 1 cut(s) 530
PspN4I GGNNCC 3 cut(s) 499, 543, 681
PspPI GGNCC 2 cut(s) 431, 829
PspPPI RGGWCCY 1 cut(s) 431
PsuI RGATCY 2 cut(s) 460, 679
RsaI GTAC 2 cut(s) 80, 617
RsaNI GTAC 2 cut(s) 79, 616
RseI CAYNNNNRTG 1 cut(s) 420
SacI GAGCTC 1 cut(s) 632
SaqAI TTAA 2 cut(s) 786, 919
SatI GCNGC 3 cut(s) 398, 675, 887
Sau3AI GATC 4 cut(s) 260, 405, 460, 679
Sau96I GGNCC 2 cut(s) 431, 829
SchI GAGTC 2 cut(s) 105, 138
ScrFI CCNGG 3 cut(s) 353, 643, 671
SduI GDGCHC 1 cut(s) 632
SfaNI GCATC 2 cut(s) 367, 589
SfcI CTRYAG 1 cut(s) 340
SinI GGWCC 2 cut(s) 431, 829
SmiMI CAYNNNNRTG 1 cut(s) 420
Sse9I AATT 3 cut(s) 327, 369, 894
SsiI CCGC 8 cut(s) 107, 204, 393, 483, 521, 677, 728, 803
SspMI CTAG 2 cut(s) 302, 548
SstI GAGCTC 1 cut(s) 632
StyD4I CCNGG 3 cut(s) 351, 641, 669
TaaI ACNGT 2 cut(s) 242, 541
TaiI ACGT 2 cut(s) 147, 321
TaqI TCGA 2 cut(s) 114, 867
TasI AATT 3 cut(s) 327, 369, 894
TatI WGTACW 1 cut(s) 615
TfiI GAWTC 3 cut(s) 5, 383, 781
Tru1I TTAA 2 cut(s) 786, 919
Tru9I TTAA 2 cut(s) 786, 919
TscAI CASTG 1 cut(s) 407
TseFI GTSAC 2 cut(s) 272, 605
TseI GCWGC 3 cut(s) 397, 674, 886
Tsp45I GTSAC 2 cut(s) 272, 605
TspDTI ATGAA 3 cut(s) 175, 285, 830
TspRI CASTG 1 cut(s) 407
VpaK11BI GGWCC 2 cut(s) 431, 829
XceI RCATGY 1 cut(s) 85
XcmI CCANNNNNNNNNTGG 2 cut(s) 145, 239
XspI CTAG 2 cut(s) 302, 548
Zsp2I ATGCAT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.