Rroxscaffold_7G00206010

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0015532
Physical Location & Seq
Reverse (-)
55659826 .. 55660737
912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00206010.1

Sequence Viewer

Length: 912 bp
ATGGATTCAAACAACAATGAAATAATCCATGACTTCCCACCTTTCTTCAAAGTCTTCAAAGATGGTCGCATAGAGAGGTACACGCGACCCCCTGGCCACGTTCCGGCGGGACCCGACCCAACAACCGGAGTCCAATCCAAAGACGTGGTGGTCTCGCCTGAAACCGGAGTAAAGGCTTGTATTTTGATCCCCAAAATCAACGGCGGAGGTCAGAAGTTGCCCATCTTAGTCCATTACCACGGTGGAGGCTTCTGCCTGGGAACAGCCTTTGAAGAAATTTTTAAGAAGTTCCTTTCATCGCTAGTCCTCAAAGCCAACGTCATTGTAGTTTCTGTTGACTACAGGCTAGCCCCAGAGCATCCTCTACCAATTGCCTACGACGATTCCTCAGCCGCTTTGCACTGGATCGCTTCCCACTCTAACGGAGAAGGGCCTGAAGCTTGGCTCAACGAGTATGCAGATCTGGACAGGGTTTTCTTGGGCGGTGAGAGTGCTGGAGCCAACATCGCTCATTATGTTGCGGTCCAAGCTGGGGTTAATGTTCCAGTGGGGTTGAACATTGTTGGCTTAGTTATGTTGCACCCCTTCTTTGGAGGTAAAGAGTGTGACAAGATGTATAAGTTTTTAAGCCCAACGAGTTCAGGGTGCGATGATGATCCGAAACTGAACCCGGCAGCGGATCCGGACTTGGTGAAATTGCCATGTAGGAAGGTGTTGGTTTGTGTTGCGGAGAAAGATCGGCTGAGAGATAGAGGAGTGAGCTACTATGAGACTCTAGAGAAACTGAAAGGGGAAGGGTTTGCGGAGTTGTTTGAGACAAAAGGAGATGATCATTGCTTTTATTTGTTCAACCCCAATGCCGACAAGGCTCGCCATCTGGTCCAAAAAGTGGTTGATTTCTTCAATATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

303

Amino Acids

33.26

Weight (kDa)

5.76

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 64 - 173 2.1e-06 Carboxylesterase family
BD-FAE PF20434 70 - 178 1.5e-11 BD-FAE
Abhydrolase_3 PF07859 76 - 281 8.3e-43 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
AccB7I CCANNNNNTGG 1 cut(s) 889
AccII CGCG 1 cut(s) 85
AccIII TCCGGA 1 cut(s) 682
AciI CCGC 8 cut(s) 107, 204, 393, 483, 521, 677, 728, 803
AclWI GGATC 5 cut(s) 181, 413, 650, 674, 687
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 1 cut(s) 276
AcuI CTGAAG 1 cut(s) 456
AfaI GTAC 1 cut(s) 80
AfiI CCNNNNNNNGG 7 cut(s) 103, 125, 164, 532, 590, 676, 889
AgsI TTSAA 7 cut(s) 9, 49, 58, 272, 556, 850, 904
AjiI CACGTC 1 cut(s) 145
AjnI CCWGG 2 cut(s) 91, 255
AluBI AGCT 3 cut(s) 440, 530, 762
AluI AGCT 3 cut(s) 440, 530, 762
Alw26I GTCTC 3 cut(s) 157, 764, 809
AlwI GGATC 5 cut(s) 181, 413, 650, 674, 687
Aor13HI TCCGGA 1 cut(s) 682
AoxI GGCC 2 cut(s) 94, 431
ApeKI GCWGC 1 cut(s) 674
ApoI RAATTY 1 cut(s) 276
ArsI GACNNNNNNTTYG 2 cut(s) 303, 335
AspS9I GGNCC 4 cut(s) 110, 431, 523, 880
AsuC2I CCSGG 1 cut(s) 671
AsuHPI GGTGA 2 cut(s) 497, 703
AsuNHI GCTAGC 1 cut(s) 346
AvaII GGWCC 3 cut(s) 110, 523, 880
BalI TGGCCA 1 cut(s) 96
BamHI GGATCC 1 cut(s) 679
BbsI GAAGAC 1 cut(s) 46
BbvCI CCTCAGC 1 cut(s) 388
BbvI GCAGC 1 cut(s) 686
BccI CCATC 3 cut(s) 56, 230, 882
BceAI ACGGC 1 cut(s) 217
BciT130I CCWGG 2 cut(s) 93, 257
BclI TGATCA 1 cut(s) 829
BcnI CCSGG 1 cut(s) 671
BcoDI GTCTC 3 cut(s) 157, 764, 809
BfaI CTAG 3 cut(s) 302, 347, 776
BfmI CTRYAG 1 cut(s) 340
BglI GCCNNNNNGGC 1 cut(s) 866
BglII AGATCT 1 cut(s) 460
BisI GCNGC 2 cut(s) 393, 675
BlsI GCNGC 2 cut(s) 394, 676
Bme1390I CCNGG 3 cut(s) 93, 257, 671
Bme18I GGWCC 3 cut(s) 110, 523, 880
BmgBI CACGTC 1 cut(s) 145
BmgT120I GGNCC 4 cut(s) 110, 431, 523, 880
BmiI GGNNCC 4 cut(s) 111, 112, 499, 681
BmrFI CCNGG 3 cut(s) 93, 257, 671
BmsI GCATC 1 cut(s) 367
BmtI GCTAGC 1 cut(s) 350
BpiI GAAGAC 1 cut(s) 46
BpmI CTGGAG 1 cut(s) 516
Bpu10I CCTNAGC 1 cut(s) 388
BpuMI CCSGG 1 cut(s) 671
BsaBI GATNNNNATC 1 cut(s) 654
BsaI GGTCTC 1 cut(s) 157
BsaJI CCNNGG 3 cut(s) 91, 238, 256
BsaWI WCCGGW 3 cut(s) 125, 164, 682
Bsc4I CCNNNNNNNGG 7 cut(s) 103, 125, 164, 532, 590, 676, 889
Bse1I ACTGG 2 cut(s) 407, 545
Bse3DI GCAATG 1 cut(s) 832
Bse8I GATNNNNATC 1 cut(s) 654
BseAI TCCGGA 1 cut(s) 682
BseBI CCWGG 2 cut(s) 93, 257
BseDI CCNNGG 3 cut(s) 91, 238, 256
BseGI GGATG 1 cut(s) 358
BseJI GATNNNNATC 1 cut(s) 654
BseLI CCNNNNNNNGG 7 cut(s) 103, 125, 164, 532, 590, 676, 889
BseMI GCAATG 1 cut(s) 832
BseMII CTCAG 2 cut(s) 402, 734
BseNI ACTGG 2 cut(s) 407, 545
BseRI GAGGAG 1 cut(s) 768
BseXI GCAGC 1 cut(s) 686
BseYI CCCAGC 1 cut(s) 530
Bsh1236I CGCG 1 cut(s) 85
BshFI GGCC 2 cut(s) 96, 433
BsiSI CCGG 5 cut(s) 104, 126, 165, 671, 683
BslFI GGGAC 1 cut(s) 123
BslI CCNNNNNNNGG 7 cut(s) 103, 125, 164, 532, 590, 676, 889
BsmAI GTCTC 3 cut(s) 157, 764, 809
BsmFI GGGAC 1 cut(s) 123
BsnI GGCC 2 cut(s) 96, 433
Bso31I GGTCTC 1 cut(s) 157
Bsp13I TCCGGA 1 cut(s) 682
Bsp143I GATC 7 cut(s) 186, 405, 460, 655, 679, 736, 829
BspACI CCGC 8 cut(s) 107, 204, 393, 483, 521, 677, 728, 803
BspANI GGCC 2 cut(s) 96, 433
BspCNI CTCAG 2 cut(s) 401, 735
BspEI TCCGGA 1 cut(s) 682
BspFNI CGCG 1 cut(s) 85
BspLI GGNNCC 4 cut(s) 111, 112, 499, 681
BspOI GCTAGC 1 cut(s) 350
BspPI GGATC 5 cut(s) 181, 413, 650, 674, 687
BspTNI GGTCTC 1 cut(s) 157
BsrDI GCAATG 1 cut(s) 832
BsrI ACTGG 2 cut(s) 407, 545
BssECI CCNNGG 3 cut(s) 91, 238, 256
BssMI GATC 7 cut(s) 186, 405, 460, 655, 679, 736, 829
Bst2UI CCWGG 2 cut(s) 93, 257
Bst4CI ACNGT 1 cut(s) 242
BstC8I GCNNGC 2 cut(s) 348, 871
BstDEI CTNAG 4 cut(s) 226, 388, 568, 743
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 1 cut(s) 358
BstFNI CGCG 1 cut(s) 85
BstKTI GATC 7 cut(s) 189, 408, 463, 658, 682, 739, 832
BstMAI GTCTC 3 cut(s) 157, 764, 809
BstMBI GATC 7 cut(s) 186, 405, 460, 655, 679, 736, 829
BstMWI GCNNNNNNNGC 3 cut(s) 506, 527, 866
BstNI CCWGG 2 cut(s) 93, 257
BstSCI CCNGG 3 cut(s) 91, 255, 669
BstSFI CTRYAG 1 cut(s) 340
BstUI CGCG 1 cut(s) 85
BstV1I GCAGC 1 cut(s) 686
BstV2I GAAGAC 1 cut(s) 46
BstX2I RGATCY 2 cut(s) 460, 679
BstXI CCANNNNNNTGG 1 cut(s) 145
BstYI RGATCY 2 cut(s) 460, 679
BsuRI GGCC 2 cut(s) 96, 433
BtgI CCRYGG 1 cut(s) 238
BtgZI GCGATG 3 cut(s) 282, 490, 663
BtrI CACGTC 1 cut(s) 145
BtsCI GGATG 1 cut(s) 358
BtsIMutI CAGTG 2 cut(s) 400, 552
Cac8I GCNNGC 2 cut(s) 348, 871
Cfr13I GGNCC 4 cut(s) 110, 431, 523, 880
Csp6I GTAC 1 cut(s) 79
CviAII CATG 2 cut(s) 29, 702
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 4 cut(s) 226, 388, 568, 743
DpnI GATC 7 cut(s) 188, 407, 462, 657, 681, 738, 831
DpnII GATC 7 cut(s) 186, 405, 460, 655, 679, 736, 829
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 1 cut(s) 94
EciI GGCGGA 1 cut(s) 219
Eco31I GGTCTC 1 cut(s) 157
Eco47I GGWCC 3 cut(s) 110, 523, 880
Eco57I CTGAAG 1 cut(s) 456
EcoO109I RGGNCCY 2 cut(s) 110, 431
EcoRII CCWGG 2 cut(s) 91, 255
FaeI CATG 2 cut(s) 32, 705
FaiI YATR 8 cut(s) 30, 71, 456, 516, 575, 618, 703, 768
FaqI GGGAC 1 cut(s) 123
FatI CATG 2 cut(s) 28, 701
FauI CCCGC 1 cut(s) 100
FbaI TGATCA 1 cut(s) 829
Fnu4HI GCNGC 2 cut(s) 393, 675
FokI GGATG 1 cut(s) 345
Fsp4HI GCNGC 2 cut(s) 393, 675
FspBI CTAG 3 cut(s) 302, 347, 776
GluI GCNGC 2 cut(s) 393, 675
GsaI CCCAGC 1 cut(s) 534
GsuI CTGGAG 1 cut(s) 516
HaeIII GGCC 2 cut(s) 96, 433
HapII CCGG 5 cut(s) 104, 126, 165, 671, 683
Hin1II CATG 2 cut(s) 32, 705
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HindIII AAGCTT 1 cut(s) 438
HinfI GANTC 4 cut(s) 5, 129, 383, 772
HpaII CCGG 5 cut(s) 104, 126, 165, 671, 683
HphI GGTGA 2 cut(s) 497, 703
Hpy166II GTNNAC 2 cut(s) 81, 337
Hpy188I TCNGA 3 cut(s) 213, 660, 911
Hpy188III TCNNGA 3 cut(s) 464, 683, 776
Hpy8I GTNNAC 2 cut(s) 81, 337
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 4 cut(s) 422, 595, 703, 788
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 3 cut(s) 99, 144, 318
HpyCH4V TGCA 3 cut(s) 400, 458, 580
HpyF10VI GCNNNNNNNGC 3 cut(s) 506, 527, 866
HpyF3I CTNAG 4 cut(s) 226, 388, 568, 743
HpySE526I ACGT 3 cut(s) 99, 144, 318
Hsp92II CATG 2 cut(s) 32, 705
KflI GGGWCCC 1 cut(s) 110
Kpn2I TCCGGA 1 cut(s) 682
Ksp22I TGATCA 1 cut(s) 829
Kzo9I GATC 7 cut(s) 186, 405, 460, 655, 679, 736, 829
LmnI GCTCC 1 cut(s) 497
Lsp1109I GCAGC 1 cut(s) 686
LweI GCATC 1 cut(s) 367
MaeI CTAG 3 cut(s) 302, 347, 776
MaeII ACGT 3 cut(s) 99, 144, 318
MaeIII GTNAC 1 cut(s) 605
MalI GATC 7 cut(s) 188, 407, 462, 657, 681, 738, 831
MboI GATC 7 cut(s) 186, 405, 460, 655, 679, 736, 829
MboII GAAGA 4 cut(s) 37, 46, 284, 892
MfeI CAATTG 1 cut(s) 369
MflI RGATCY 2 cut(s) 460, 679
MlsI TGGCCA 1 cut(s) 96
MluCI AATT 3 cut(s) 276, 369, 695
MluNI TGGCCA 1 cut(s) 96
MlyI GAGTC 2 cut(s) 138, 766
MnlI CCTC 8 cut(s) 69, 200, 239, 317, 372, 397, 587, 746
Mox20I TGGCCA 1 cut(s) 96
MroI TCCGGA 1 cut(s) 682
MscI TGGCCA 1 cut(s) 96
MseI TTAA 3 cut(s) 282, 537, 626
Msp20I TGGCCA 1 cut(s) 96
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 5 cut(s) 104, 126, 165, 671, 683
MspR9I CCNGG 3 cut(s) 93, 257, 671
MunI CAATTG 1 cut(s) 369
MvaI CCWGG 2 cut(s) 93, 257
MvnI CGCG 1 cut(s) 85
MwoI GCNNNNNNNGC 3 cut(s) 506, 527, 866
NciI CCSGG 1 cut(s) 671
NdeII GATC 7 cut(s) 186, 405, 460, 655, 679, 736, 829
NheI GCTAGC 1 cut(s) 346
NlaIII CATG 2 cut(s) 32, 705
NlaIV GGNNCC 4 cut(s) 111, 112, 499, 681
NmuCI GTSAC 1 cut(s) 605
PfeI GAWTC 2 cut(s) 5, 383
PflMI CCANNNNNTGG 1 cut(s) 889
PkrI GCNGC 2 cut(s) 394, 676
PleI GAGTC 2 cut(s) 137, 766
PpsI GAGTC 2 cut(s) 137, 766
PpuMI RGGWCCY 1 cut(s) 110
Psp5II RGGWCCY 1 cut(s) 110
Psp6I CCWGG 2 cut(s) 91, 255
PspFI CCCAGC 1 cut(s) 530
PspGI CCWGG 2 cut(s) 91, 255
PspN4I GGNNCC 4 cut(s) 111, 112, 499, 681
PspPI GGNCC 4 cut(s) 110, 431, 523, 880
PspPPI RGGWCCY 1 cut(s) 110
PsuI RGATCY 2 cut(s) 460, 679
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
SaqAI TTAA 3 cut(s) 282, 537, 626
SatI GCNGC 2 cut(s) 393, 675
Sau3AI GATC 7 cut(s) 186, 405, 460, 655, 679, 736, 829
Sau96I GGNCC 4 cut(s) 110, 431, 523, 880
SchI GAGTC 2 cut(s) 138, 766
ScrFI CCNGG 3 cut(s) 93, 257, 671
SfaNI GCATC 1 cut(s) 367
SfcI CTRYAG 1 cut(s) 340
SinI GGWCC 3 cut(s) 110, 523, 880
Sse9I AATT 3 cut(s) 276, 369, 695
SsiI CCGC 8 cut(s) 107, 204, 393, 483, 521, 677, 728, 803
SspMI CTAG 3 cut(s) 302, 347, 776
StyD4I CCNGG 3 cut(s) 91, 255, 669
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 3 cut(s) 102, 147, 321
TasI AATT 3 cut(s) 276, 369, 695
TauI GCSGC 1 cut(s) 395
TfiI GAWTC 2 cut(s) 5, 383
Tru1I TTAA 3 cut(s) 282, 537, 626
Tru9I TTAA 3 cut(s) 282, 537, 626
TscAI CASTG 2 cut(s) 407, 552
TseFI GTSAC 1 cut(s) 605
TseI GCWGC 1 cut(s) 674
Tsp45I GTSAC 1 cut(s) 605
TspDTI ATGAA 2 cut(s) 33, 285
TspGWI ACGGA 1 cut(s) 438
TspRI CASTG 2 cut(s) 407, 552
Van91I CCANNNNNTGG 1 cut(s) 889
VpaK11BI GGWCC 3 cut(s) 110, 523, 880
XapI RAATTY 1 cut(s) 276
XbaI TCTAGA 1 cut(s) 775
XcmI CCANNNNNNNNNTGG 2 cut(s) 145, 239
XspI CTAG 3 cut(s) 302, 347, 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.