Rw6G008590

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0015532
Physical Location & Seq
Reverse (-)
14534235 .. 14535146
912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G008590.1

Sequence Viewer

Length: 912 bp
ATGGATTCAAACAACAACGAAATAATCCATGACTTCCCACCATTCTTCAAAGTCTTCAAAGATGGTACCATAGAGCGGTACATGCCACTCCATGGCCACGTTCCGGCGGGACTCGACCCAACAACCGGAGTCCAATCCAAAGACGTGGTGGTCTCGCCTGAAACCGGAGTAAAGGCTCGTATTTTCATCCCCAAAATCAACGGCGGAAGTCAGAAGTTGCCCCTTTTAGTCCATTACCACGGTGGAGGCTTCTGCATGGGATCACCCTTCGGTGAAATTGGAAAGAAGTTCCTTTCGTCTCTAGTACTCAAAGCCAACATCATTGTAGTTTCTGTTGACTACAGGCTAGCCCCAGAGCATCCTCTACCAATTGCCTACGACGATTCCTTAGCCGCTTTGCACTGGATCGCTTCCCACTCTAATATTCAAGGGCCTGAAGCTTGGCTGAATGAGTATGCAGATCTGGACAGGGTTTTCTTGGGCGGTGAGAGTGCTGGAGCCAACATCGCTCATTATGTTGCGGTCCAAGCTGGGGTTAATGTTCCAGTGGGGTTGAACATTGTTGGCTTAGTTATGGTGCACCCCTTCTTTGGAGGTACAGAGTGCGACAAGATGTACAAGTTTTTAAGCTCAACGAGTTCAGGCTGCGATGATGATCCGAAACTGAACCCGGCAGCGGATCCGGACCTGGTAAAATTGCCATGTAGGAAGGTGTTGGTTTGTGTTGCGGAGAAAGATTGGCTGAGAGATAGAGGAGTGAGCTACCTTGAGACTCTAGAGAAACTGAAAGGGGAAGGGTTTGCGGAGTTGTTTGAGACAAAAGGAGAGGACCATTGCTTTCATTTGTTCAACCCCAATGCCGACAAGGCTGGCCATCTGCTGCAGAAAGTGGTTGATTTCTTCAATATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

303

Amino Acids

33.16

Weight (kDa)

5.68

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 61 - 174 1.9e-07 Carboxylesterase family
BD-FAE PF20434 68 - 178 4.2e-11 BD-FAE
Abhydrolase_3 PF07859 76 - 281 1.7e-44 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
Acc65I GGTACC 1 cut(s) 65
AccB1I GGYRCC 1 cut(s) 65
AccB7I CCANNNNNTGG 1 cut(s) 92
AccBSI CCGCTC 1 cut(s) 76
AccIII TCCGGA 1 cut(s) 682
AciI CCGC 9 cut(s) 76, 107, 204, 393, 483, 521, 677, 728, 803
AclWI GGATC 5 cut(s) 268, 413, 650, 674, 687
AcoI YGGCCR 2 cut(s) 94, 871
AcuI CTGAAG 1 cut(s) 456
AfaI GTAC 5 cut(s) 67, 80, 306, 598, 617
AfiI CCNNNNNNNGG 8 cut(s) 75, 92, 103, 125, 164, 532, 590, 676
AgsI TTSAA 7 cut(s) 9, 49, 58, 428, 556, 850, 904
AjiI CACGTC 1 cut(s) 145
AjnI CCWGG 1 cut(s) 687
AluBI AGCT 4 cut(s) 440, 530, 630, 762
AluI AGCT 4 cut(s) 440, 530, 630, 762
Alw21I GWGCWC 1 cut(s) 582
Alw26I GTCTC 4 cut(s) 157, 303, 764, 809
Alw44I GTGCAC 1 cut(s) 578
AlwI GGATC 5 cut(s) 268, 413, 650, 674, 687
Aor13HI TCCGGA 1 cut(s) 682
AoxI GGCC 3 cut(s) 94, 431, 871
ApaLI GTGCAC 1 cut(s) 578
ApeKI GCWGC 3 cut(s) 645, 674, 880
Asp718I GGTACC 1 cut(s) 65
AspS9I GGNCC 4 cut(s) 431, 523, 685, 829
AsuC2I CCSGG 1 cut(s) 671
AsuHPI GGTGA 3 cut(s) 255, 284, 497
AsuNHI GCTAGC 1 cut(s) 346
AvaII GGWCC 3 cut(s) 523, 685, 829
BaeGI GKGCMC 1 cut(s) 582
BalI TGGCCA 2 cut(s) 96, 873
BamHI GGATCC 1 cut(s) 679
BanI GGYRCC 1 cut(s) 65
BbsI GAAGAC 1 cut(s) 46
Bbv12I GWGCWC 1 cut(s) 582
BbvI GCAGC 3 cut(s) 632, 686, 867
BccI CCATC 2 cut(s) 56, 882
BceAI ACGGC 1 cut(s) 217
BciT130I CCWGG 1 cut(s) 689
BcnI CCSGG 1 cut(s) 671
BcoDI GTCTC 4 cut(s) 157, 303, 764, 809
BfaI CTAG 3 cut(s) 302, 347, 776
BfmI CTRYAG 2 cut(s) 340, 881
BglI GCCNNNNNGGC 1 cut(s) 866
BglII AGATCT 1 cut(s) 460
BisI GCNGC 4 cut(s) 393, 646, 675, 881
BlsI GCNGC 4 cut(s) 394, 647, 676, 882
BmcAI AGTACT 1 cut(s) 306
Bme1390I CCNGG 2 cut(s) 671, 689
Bme18I GGWCC 3 cut(s) 523, 685, 829
BmgBI CACGTC 1 cut(s) 145
BmgT120I GGNCC 4 cut(s) 431, 523, 685, 829
BmiI GGNNCC 3 cut(s) 67, 499, 681
BmrFI CCNGG 2 cut(s) 671, 689
BmsI GCATC 1 cut(s) 367
BmtI GCTAGC 1 cut(s) 350
BpiI GAAGAC 1 cut(s) 46
BpmI CTGGAG 1 cut(s) 516
Bpu10I CCTNAGC 1 cut(s) 388
BpuEI CTTGAG 1 cut(s) 788
BpuMI CCSGG 1 cut(s) 671
BsaBI GATNNNNATC 1 cut(s) 654
BsaI GGTCTC 1 cut(s) 157
BsaJI CCNNGG 2 cut(s) 91, 238
BsaWI WCCGGW 3 cut(s) 125, 164, 682
Bsc4I CCNNNNNNNGG 8 cut(s) 75, 92, 103, 125, 164, 532, 590, 676
Bse1I ACTGG 2 cut(s) 407, 545
Bse3DI GCAATG 1 cut(s) 832
Bse8I GATNNNNATC 1 cut(s) 654
BseAI TCCGGA 1 cut(s) 682
BseBI CCWGG 1 cut(s) 689
BseDI CCNNGG 2 cut(s) 91, 238
BseGI GGATG 2 cut(s) 186, 358
BseJI GATNNNNATC 1 cut(s) 654
BseLI CCNNNNNNNGG 8 cut(s) 75, 92, 103, 125, 164, 532, 590, 676
BseMI GCAATG 1 cut(s) 832
BseMII CTCAG 1 cut(s) 734
BseNI ACTGG 2 cut(s) 407, 545
BseRI GAGGAG 1 cut(s) 768
BseSI GKGCMC 1 cut(s) 582
BseXI GCAGC 3 cut(s) 632, 686, 867
BseYI CCCAGC 1 cut(s) 530
BshFI GGCC 3 cut(s) 96, 433, 873
BshNI GGYRCC 1 cut(s) 65
BsiHKAI GWGCWC 1 cut(s) 582
BsiSI CCGG 5 cut(s) 104, 126, 165, 671, 683
BslFI GGGAC 1 cut(s) 123
BslI CCNNNNNNNGG 8 cut(s) 75, 92, 103, 125, 164, 532, 590, 676
BsmAI GTCTC 4 cut(s) 157, 303, 764, 809
BsmBI CGTCTC 1 cut(s) 303
BsmFI GGGAC 1 cut(s) 123
BsnI GGCC 3 cut(s) 96, 433, 873
Bso31I GGTCTC 1 cut(s) 157
Bsp1286I GDGCHC 1 cut(s) 582
Bsp13I TCCGGA 1 cut(s) 682
Bsp1407I TGTACA 1 cut(s) 615
Bsp143I GATC 5 cut(s) 260, 405, 460, 655, 679
Bsp19I CCATGG 1 cut(s) 91
BspACI CCGC 9 cut(s) 76, 107, 204, 393, 483, 521, 677, 728, 803
BspANI GGCC 3 cut(s) 96, 433, 873
BspCNI CTCAG 1 cut(s) 735
BspEI TCCGGA 1 cut(s) 682
BspLI GGNNCC 3 cut(s) 67, 499, 681
BspMAI CTGCAG 1 cut(s) 885
BspOI GCTAGC 1 cut(s) 350
BspPI GGATC 5 cut(s) 268, 413, 650, 674, 687
BspT107I GGYRCC 1 cut(s) 65
BspTNI GGTCTC 1 cut(s) 157
BsrBI CCGCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 832
BsrGI TGTACA 1 cut(s) 615
BsrI ACTGG 2 cut(s) 407, 545
BssECI CCNNGG 2 cut(s) 91, 238
BssMI GATC 5 cut(s) 260, 405, 460, 655, 679
BssT1I CCWWGG 1 cut(s) 91
Bst2UI CCWGG 1 cut(s) 689
Bst4CI ACNGT 1 cut(s) 242
BstAUI TGTACA 1 cut(s) 615
BstC8I GCNNGC 2 cut(s) 348, 871
BstDEI CTNAG 3 cut(s) 388, 568, 743
BstDSI CCRYGG 2 cut(s) 91, 238
BstF5I GGATG 2 cut(s) 186, 358
BstKTI GATC 5 cut(s) 263, 408, 463, 658, 682
BstMAI GTCTC 4 cut(s) 157, 303, 764, 809
BstMBI GATC 5 cut(s) 260, 405, 460, 655, 679
BstMWI GCNNNNNNNGC 4 cut(s) 82, 506, 527, 866
BstNI CCWGG 1 cut(s) 689
BstNSI RCATGY 1 cut(s) 85
BstSCI CCNGG 2 cut(s) 669, 687
BstSFI CTRYAG 2 cut(s) 340, 881
BstSLI GKGCMC 1 cut(s) 582
BstV1I GCAGC 3 cut(s) 632, 686, 867
BstV2I GAAGAC 1 cut(s) 46
BstX2I RGATCY 2 cut(s) 460, 679
BstXI CCANNNNNNTGG 1 cut(s) 145
BstYI RGATCY 2 cut(s) 460, 679
BsuRI GGCC 3 cut(s) 96, 433, 873
BtgI CCRYGG 2 cut(s) 91, 238
BtgZI GCGATG 2 cut(s) 490, 663
BtrI CACGTC 1 cut(s) 145
BtsCI GGATG 2 cut(s) 186, 358
BtsIMutI CAGTG 2 cut(s) 400, 552
Cac8I GCNNGC 2 cut(s) 348, 871
Cfr13I GGNCC 4 cut(s) 431, 523, 685, 829
CsiI ACCWGGT 1 cut(s) 687
Csp6I GTAC 5 cut(s) 66, 79, 305, 597, 616
CviAII CATG 5 cut(s) 29, 82, 92, 256, 702
CviQI GTAC 5 cut(s) 66, 79, 305, 597, 616
DdeI CTNAG 3 cut(s) 388, 568, 743
DpnI GATC 5 cut(s) 262, 407, 462, 657, 681
DpnII GATC 5 cut(s) 260, 405, 460, 655, 679
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 2 cut(s) 94, 871
EciI GGCGGA 1 cut(s) 219
Eco130I CCWWGG 1 cut(s) 91
Eco31I GGTCTC 1 cut(s) 157
Eco47I GGWCC 3 cut(s) 523, 685, 829
Eco57I CTGAAG 1 cut(s) 456
EcoO109I RGGNCCY 1 cut(s) 431
EcoRII CCWGG 1 cut(s) 687
EcoT14I CCWWGG 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 91
Esp3I CGTCTC 1 cut(s) 303
FaeI CATG 5 cut(s) 32, 85, 95, 259, 705
FaiI YATR 9 cut(s) 30, 71, 83, 93, 257, 456, 516, 575, 703
FaqI GGGAC 1 cut(s) 123
FatI CATG 5 cut(s) 28, 81, 91, 255, 701
FauI CCCGC 1 cut(s) 100
Fnu4HI GCNGC 4 cut(s) 393, 646, 675, 881
FokI GGATG 2 cut(s) 173, 345
Fsp4HI GCNGC 4 cut(s) 393, 646, 675, 881
FspBI CTAG 3 cut(s) 302, 347, 776
GluI GCNGC 4 cut(s) 393, 646, 675, 881
GsaI CCCAGC 1 cut(s) 534
GsuI CTGGAG 1 cut(s) 516
HaeIII GGCC 3 cut(s) 96, 433, 873
HapII CCGG 5 cut(s) 104, 126, 165, 671, 683
Hin1II CATG 5 cut(s) 32, 85, 95, 259, 705
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HindIII AAGCTT 1 cut(s) 438
HinfI GANTC 5 cut(s) 5, 111, 129, 383, 772
HpaII CCGG 5 cut(s) 104, 126, 165, 671, 683
HphI GGTGA 3 cut(s) 255, 284, 497
Hpy166II GTNNAC 2 cut(s) 337, 580
Hpy188I TCNGA 3 cut(s) 213, 660, 911
Hpy188III TCNNGA 3 cut(s) 464, 683, 776
Hpy8I GTNNAC 2 cut(s) 337, 580
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 4 cut(s) 277, 595, 703, 788
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 2 cut(s) 99, 144
HpyCH4V TGCA 5 cut(s) 255, 400, 458, 580, 883
HpyF10VI GCNNNNNNNGC 4 cut(s) 82, 506, 527, 866
HpyF3I CTNAG 3 cut(s) 388, 568, 743
HpySE526I ACGT 2 cut(s) 99, 144
Hsp92II CATG 5 cut(s) 32, 85, 95, 259, 705
Kpn2I TCCGGA 1 cut(s) 682
KpnI GGTACC 1 cut(s) 69
Kzo9I GATC 5 cut(s) 260, 405, 460, 655, 679
LmnI GCTCC 1 cut(s) 497
Lsp1109I GCAGC 3 cut(s) 632, 686, 867
LweI GCATC 1 cut(s) 367
MabI ACCWGGT 1 cut(s) 687
MaeI CTAG 3 cut(s) 302, 347, 776
MaeII ACGT 2 cut(s) 99, 144
MalI GATC 5 cut(s) 262, 407, 462, 657, 681
MbiI CCGCTC 1 cut(s) 76
MboI GATC 5 cut(s) 260, 405, 460, 655, 679
MboII GAAGA 3 cut(s) 37, 46, 892
MfeI CAATTG 1 cut(s) 369
MflI RGATCY 2 cut(s) 460, 679
MhlI GDGCHC 1 cut(s) 582
MlsI TGGCCA 2 cut(s) 96, 873
MluCI AATT 3 cut(s) 276, 369, 695
MluNI TGGCCA 2 cut(s) 96, 873
MlyI GAGTC 3 cut(s) 105, 138, 766
MnlI CCTC 5 cut(s) 239, 372, 587, 746, 820
Mox20I TGGCCA 2 cut(s) 96, 873
MroI TCCGGA 1 cut(s) 682
MscI TGGCCA 2 cut(s) 96, 873
MseI TTAA 2 cut(s) 537, 626
Msp20I TGGCCA 2 cut(s) 96, 873
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 5 cut(s) 104, 126, 165, 671, 683
MspR9I CCNGG 2 cut(s) 671, 689
MunI CAATTG 1 cut(s) 369
MvaI CCWGG 1 cut(s) 689
MwoI GCNNNNNNNGC 4 cut(s) 82, 506, 527, 866
NciI CCSGG 1 cut(s) 671
NcoI CCATGG 1 cut(s) 91
NdeII GATC 5 cut(s) 260, 405, 460, 655, 679
NheI GCTAGC 1 cut(s) 346
NlaIII CATG 5 cut(s) 32, 85, 95, 259, 705
NlaIV GGNNCC 3 cut(s) 67, 499, 681
NspI RCATGY 1 cut(s) 85
PfeI GAWTC 2 cut(s) 5, 383
PflMI CCANNNNNTGG 1 cut(s) 92
PkrI GCNGC 4 cut(s) 394, 647, 676, 882
PleI GAGTC 3 cut(s) 105, 137, 766
PpsI GAGTC 3 cut(s) 105, 137, 766
Psp6I CCWGG 1 cut(s) 687
PspFI CCCAGC 1 cut(s) 530
PspGI CCWGG 1 cut(s) 687
PspN4I GGNNCC 3 cut(s) 67, 499, 681
PspPI GGNCC 4 cut(s) 431, 523, 685, 829
PstI CTGCAG 1 cut(s) 885
PsuI RGATCY 2 cut(s) 460, 679
RsaI GTAC 5 cut(s) 67, 80, 306, 598, 617
RsaNI GTAC 5 cut(s) 66, 79, 305, 597, 616
SaqAI TTAA 2 cut(s) 537, 626
SatI GCNGC 4 cut(s) 393, 646, 675, 881
Sau3AI GATC 5 cut(s) 260, 405, 460, 655, 679
Sau96I GGNCC 4 cut(s) 431, 523, 685, 829
ScaI AGTACT 1 cut(s) 306
SchI GAGTC 3 cut(s) 105, 138, 766
ScrFI CCNGG 2 cut(s) 671, 689
SduI GDGCHC 1 cut(s) 582
SexAI ACCWGGT 1 cut(s) 687
SfaNI GCATC 1 cut(s) 367
SfcI CTRYAG 2 cut(s) 340, 881
SinI GGWCC 3 cut(s) 523, 685, 829
SmlI CTYRAG 1 cut(s) 767
SmoI CTYRAG 1 cut(s) 767
Sse9I AATT 3 cut(s) 276, 369, 695
SsiI CCGC 9 cut(s) 76, 107, 204, 393, 483, 521, 677, 728, 803
SspI AATATT 1 cut(s) 424
SspMI CTAG 3 cut(s) 302, 347, 776
StyD4I CCNGG 2 cut(s) 669, 687
StyI CCWWGG 1 cut(s) 91
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 2 cut(s) 102, 147
TaqI TCGA 1 cut(s) 114
TasI AATT 3 cut(s) 276, 369, 695
TatI WGTACW 2 cut(s) 304, 615
TauI GCSGC 1 cut(s) 395
TfiI GAWTC 2 cut(s) 5, 383
Tru1I TTAA 2 cut(s) 537, 626
Tru9I TTAA 2 cut(s) 537, 626
TscAI CASTG 2 cut(s) 407, 552
TseI GCWGC 3 cut(s) 645, 674, 880
TspDTI ATGAA 2 cut(s) 175, 830
TspRI CASTG 2 cut(s) 407, 552
Van91I CCANNNNNTGG 1 cut(s) 92
VneI GTGCAC 1 cut(s) 578
VpaK11BI GGWCC 3 cut(s) 523, 685, 829
XbaI TCTAGA 1 cut(s) 775
XceI RCATGY 1 cut(s) 85
XcmI CCANNNNNNNNNTGG 2 cut(s) 145, 239
XspI CTAG 3 cut(s) 302, 347, 776
ZrmI AGTACT 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.