FvH4_2g07750

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014652
Physical Location & Seq
Forward (+)
6678450 .. 6678983
534 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g07750.t1

Sequence Viewer

Length: 534 bp
ATGGGCATTAGCAGTTCTTCATCAACCTCTGTGACCACAACCAAGCTGGTTCTCCCAGATGGGCAGCTCCAAGAATTCTCAAACCCAATTAGGGCATCCTACGTACTGCAGAAGATCAGTTCCCCGGGATTTGTATGCGATGCCGACGACATGGATTTCGGAGGCTTTATTACGGCTGTTAACGGCGACGAAGAGCTTCAGCTGGGTCAGCTTTACTTTGTGTTGCCTTTGAGTTGGTTGAATAAGCCGCTTCGGGCGGAGGAGATGGCGGCTTTGGCTGTTAGGGCAAGTTTGGCGCTTGGCAATATGAGACGGAGTAATAGGTGTTGTTGTAAAGGAGGGGATGATCAAGTTGTATTTAGTATTATTAAGAAGGATGTGATCAAGCCTAAGATGGAGGCGGGCGGCGGCAGTTTTGGTGTTCATCGAGAAACAGTGAGGAAAAGAAGAGGGAGGAGCGGACGCCACGGTGGTGGTGGAAGTTATAGTTCTGTGGCAAAGTTGAGTGTGATTTTGGAAGACGAGGATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

178

Amino Acids

19.06

Weight (kDa)

8.37

Isoelectric Point (pI)

51.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 108 1e-24 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014652)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g07750
malus_domestica MD10G1084300.v1.1
prunus_persica Prupe.8G107500_v2.0.a1
pyrus_communis pycom10g06630
rosa_chinensis RchiOBHm_Chr6g0262101 RchiOBHm_Chr6g0271951
rosa_laevigata RLG00000014453
rosa_multiflora Rmu_sc0002510.1_g000007 Rmu_ssc0000125.1_g000021
rosa_roxburghii Rroxscaffold_7G00207780 Rroxscaffold_7G00207820
rosa_rugosa Rorug05G0595200
rosa_samantha Rh6AG115000 Rh6BG109000 Rh6CG106300 Rh6DG096700
rosa_wichuraiana Rw6G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 459
AciI CCGC 7 cut(s) 248, 257, 269, 401, 405, 408, 459
AcsI RAATTY 1 cut(s) 74
AcuI CTGAAG 1 cut(s) 182
AcyI GRCGYC 1 cut(s) 463
AfaI GTAC 1 cut(s) 105
AfiI CCNNNNNNNGG 1 cut(s) 91
AgsI TTSAA 1 cut(s) 241
AleI CACNNNNGTG 1 cut(s) 471
AluBI AGCT 5 cut(s) 46, 67, 196, 202, 211
AluI AGCT 5 cut(s) 46, 67, 196, 202, 211
Alw26I GTCTC 1 cut(s) 304
Ama87I CYCGRG 1 cut(s) 124
ApeKI GCWGC 1 cut(s) 64
ApoI RAATTY 1 cut(s) 74
ArsI GACNNNNNNTTYG 2 cut(s) 140, 172
Asp700I GAANNNNTTC 1 cut(s) 195
AspLEI GCGC 1 cut(s) 298
AsuC2I CCSGG 2 cut(s) 125, 126
AvaI CYCGRG 1 cut(s) 124
BbsI GAAGAC 1 cut(s) 525
BbvI GCAGC 1 cut(s) 76
BccI CCATC 3 cut(s) 53, 259, 388
BceAI ACGGC 2 cut(s) 189, 199
BclI TGATCA 2 cut(s) 346, 381
BcnI CCSGG 2 cut(s) 125, 126
BcoDI GTCTC 1 cut(s) 304
BfmI CTRYAG 1 cut(s) 107
BfoI RGCGCY 1 cut(s) 299
BisI GCNGC 5 cut(s) 65, 248, 270, 406, 409
BlsI GCNGC 5 cut(s) 66, 249, 271, 407, 410
Bme1390I CCNGG 2 cut(s) 125, 126
BmeT110I CYCGRG 1 cut(s) 124
BmrFI CCNGG 2 cut(s) 125, 126
BmsI GCATC 2 cut(s) 104, 130
BpiI GAAGAC 1 cut(s) 525
BpuMI CCSGG 2 cut(s) 125, 126
BsaAI YACGTR 1 cut(s) 103
BsaHI GRCGYC 1 cut(s) 463
BsaJI CCNNGG 3 cut(s) 123, 124, 466
Bsc4I CCNNNNNNNGG 1 cut(s) 91
BseDI CCNNGG 3 cut(s) 123, 124, 466
BseGI GGATG 3 cut(s) 95, 349, 382
BseLI CCNNNNNNNGG 1 cut(s) 91
BseRI GAGGAG 2 cut(s) 275, 469
BseXI GCAGC 1 cut(s) 76
BseYI CCCAGC 1 cut(s) 202
BsiHKCI CYCGRG 1 cut(s) 124
BsiSI CCGG 1 cut(s) 125
BslI CCNNNNNNNGG 1 cut(s) 91
BsmAI GTCTC 1 cut(s) 304
BsmBI CGTCTC 1 cut(s) 304
BsoBI CYCGRG 1 cut(s) 124
Bsp143I GATC 3 cut(s) 114, 346, 381
BspACI CCGC 7 cut(s) 248, 257, 269, 401, 405, 408, 459
BspMAI CTGCAG 1 cut(s) 111
BspQI GCTCTTC 1 cut(s) 186
BsrBI CCGCTC 1 cut(s) 459
BssECI CCNNGG 3 cut(s) 123, 124, 466
BssMI GATC 3 cut(s) 114, 346, 381
BssNI GRCGYC 1 cut(s) 463
Bst4CI ACNGT 2 cut(s) 436, 470
Bst6I CTCTTC 2 cut(s) 186, 442
BstACI GRCGYC 1 cut(s) 463
BstBAI YACGTR 1 cut(s) 103
BstC8I GCNNGC 1 cut(s) 403
BstDEI CTNAG 1 cut(s) 390
BstDSI CCRYGG 1 cut(s) 466
BstF5I GGATG 3 cut(s) 95, 349, 382
BstH2I RGCGCY 1 cut(s) 299
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 3 cut(s) 117, 349, 384
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 3 cut(s) 114, 346, 381
BstMWI GCNNNNNNNGC 4 cut(s) 208, 275, 284, 293
BstSCI CCNGG 2 cut(s) 123, 124
BstSFI CTRYAG 1 cut(s) 107
BstSNI TACGTA 1 cut(s) 103
BstV1I GCAGC 1 cut(s) 76
BstV2I GAAGAC 1 cut(s) 525
BstXI CCANNNNNNTGG 1 cut(s) 473
BtgI CCRYGG 1 cut(s) 466
BtgZI GCGATG 1 cut(s) 153
BtsCI GGATG 3 cut(s) 95, 349, 382
BtsIMutI CAGTG 1 cut(s) 441
Cac8I GCNNGC 1 cut(s) 403
CfoI GCGC 1 cut(s) 298
Cfr9I CCCGGG 1 cut(s) 124
CseI GACGC 1 cut(s) 471
Csp6I GTAC 1 cut(s) 104
CviAII CATG 1 cut(s) 151
CviQI GTAC 1 cut(s) 104
DdeI CTNAG 1 cut(s) 390
DpnI GATC 3 cut(s) 116, 348, 383
DpnII GATC 3 cut(s) 114, 346, 381
Eam1104I CTCTTC 2 cut(s) 186, 442
EarI CTCTTC 2 cut(s) 186, 442
EciI GGCGGA 1 cut(s) 272
Eco105I TACGTA 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 182
Eco88I CYCGRG 1 cut(s) 124
EcoRI GAATTC 1 cut(s) 74
Esp3I CGTCTC 1 cut(s) 304
FaeI CATG 1 cut(s) 154
FaiI YATR 6 cut(s) 136, 152, 308, 486, 530, 532
FatI CATG 1 cut(s) 150
FauI CCCGC 1 cut(s) 394
FbaI TGATCA 2 cut(s) 346, 381
Fnu4HI GCNGC 5 cut(s) 65, 248, 270, 406, 409
FokI GGATG 3 cut(s) 82, 356, 389
Fsp4HI GCNGC 5 cut(s) 65, 248, 270, 406, 409
GlaI GCGC 1 cut(s) 297
GluI GCNGC 5 cut(s) 65, 248, 270, 406, 409
GsaI CCCAGC 1 cut(s) 206
HaeII RGCGCY 1 cut(s) 299
HapII CCGG 1 cut(s) 125
HgaI GACGC 1 cut(s) 471
HhaI GCGC 1 cut(s) 298
Hin1I GRCGYC 1 cut(s) 463
Hin1II CATG 1 cut(s) 154
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HpaI GTTAAC 1 cut(s) 181
HpaII CCGG 1 cut(s) 125
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 1 cut(s) 161
Hpy188III TCNNGA 1 cut(s) 428
Hpy8I GTNNAC 1 cut(s) 181
Hpy99I CGWCG 2 cut(s) 149, 191
HpyAV CCTTC 1 cut(s) 367
HpyCH4III ACNGT 2 cut(s) 436, 470
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 1 cut(s) 109
HpyF10VI GCNNNNNNNGC 4 cut(s) 208, 275, 284, 293
HpyF3I CTNAG 1 cut(s) 390
HpySE526I ACGT 1 cut(s) 102
Hsp92I GRCGYC 1 cut(s) 463
Hsp92II CATG 1 cut(s) 154
HspAI GCGC 1 cut(s) 296
Ksp22I TGATCA 2 cut(s) 346, 381
KspAI GTTAAC 1 cut(s) 181
Kzo9I GATC 3 cut(s) 114, 346, 381
LguI GCTCTTC 1 cut(s) 186
LmnI GCTCC 2 cut(s) 72, 456
LpnPI CCDG 4 cut(s) 32, 69, 138, 188
Lsp1109I GCAGC 1 cut(s) 76
LweI GCATC 2 cut(s) 104, 130
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 1 cut(s) 31
MalI GATC 3 cut(s) 116, 348, 383
MbiI CCGCTC 1 cut(s) 459
MboI GATC 3 cut(s) 114, 346, 381
MboII GAAGA 5 cut(s) 9, 124, 203, 459, 530
MluCI AATT 2 cut(s) 74, 87
MnlI CCTC 9 cut(s) 37, 155, 253, 332, 391, 432, 443, 447, 517
MroXI GAANNNNTTC 1 cut(s) 195
MseI TTAA 2 cut(s) 180, 369
MslI CAYNNNNRTG 1 cut(s) 471
MspA1I CMGCKG 1 cut(s) 202
MspI CCGG 1 cut(s) 125
MspR9I CCNGG 2 cut(s) 125, 126
MwoI GCNNNNNNNGC 4 cut(s) 208, 275, 284, 293
NciI CCSGG 2 cut(s) 125, 126
NdeII GATC 3 cut(s) 114, 346, 381
NlaIII CATG 1 cut(s) 154
NmuCI GTSAC 1 cut(s) 31
OliI CACNNNNGTG 1 cut(s) 471
PciSI GCTCTTC 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 195
PkrI GCNGC 5 cut(s) 66, 249, 271, 407, 410
Ppu21I YACGTR 1 cut(s) 103
PspFI CCCAGC 1 cut(s) 202
PstI CTGCAG 1 cut(s) 111
PvuII CAGCTG 1 cut(s) 202
RsaI GTAC 1 cut(s) 105
RsaNI GTAC 1 cut(s) 104
RseI CAYNNNNRTG 1 cut(s) 471
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 2 cut(s) 180, 369
SatI GCNGC 5 cut(s) 65, 248, 270, 406, 409
Sau3AI GATC 3 cut(s) 114, 346, 381
ScrFI CCNGG 2 cut(s) 125, 126
SetI ASST 8 cut(s) 29, 48, 69, 105, 198, 204, 213, 326
SfaNI GCATC 2 cut(s) 104, 130
SfcI CTRYAG 1 cut(s) 107
SmaI CCCGGG 1 cut(s) 126
SmiMI CAYNNNNRTG 1 cut(s) 471
SnaBI TACGTA 1 cut(s) 103
Sse9I AATT 2 cut(s) 74, 87
SsiI CCGC 7 cut(s) 248, 257, 269, 401, 405, 408, 459
StyD4I CCNGG 2 cut(s) 123, 124
TaaI ACNGT 2 cut(s) 436, 470
TaiI ACGT 1 cut(s) 105
TaqI TCGA 1 cut(s) 427
TasI AATT 2 cut(s) 74, 87
TauI GCSGC 4 cut(s) 250, 272, 408, 411
Tru1I TTAA 2 cut(s) 180, 369
Tru9I TTAA 2 cut(s) 180, 369
TscAI CASTG 1 cut(s) 441
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 1 cut(s) 64
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 2 cut(s) 9, 413
TspGWI ACGGA 1 cut(s) 328
TspMI CCCGGG 1 cut(s) 124
TspRI CASTG 1 cut(s) 441
XapI RAATTY 1 cut(s) 74
XcmI CCANNNNNNNNNTGG 2 cut(s) 43, 473
XmaI CCCGGG 1 cut(s) 124
XmnI GAANNNNTTC 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.