pycom10g06630

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0014652
Physical Location & Seq
Reverse (-)
7746051 .. 7746623
573 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g06630.1

Sequence Viewer

Length: 573 bp
ATGGGGATTTGCAACTCATGCGAATCAATCTCTGTAGCTACAGCCAAGCTGATTCTTCAAAACGGGCAGCTTCAAGAATTCTCGAGACCGATTAAGGTCTCACATGCATTGCAGATGATGAACGTCAGTCCCATGCATTTCATATGCGACTCAGATGACATGGAGTTTGGAGGCTTTTTTGGTGCTGTTGATGGGGATCAAGTGCTTCAGTTGGGTCAGCTTTACTTTGTGTTACCGTTGAGTTGGCTGAAAAAACCACTTCGACCGGAGCAGATGGCTGCTTTGGCCGTTAGGGCAAGTTTGGCGCTTACAAATATGAGACGGAGATATGGAGGCGGGTGTAAAAGTGCCAGTCCAGTTGTGTTTACTATTAAGAAGAATGTGAAGACCAGTCGGATGGCGGCCATGGGAGGTGGTGACAGCGGCATAAGCGATGGAGTCAGACGTCCTGAAGTTGTGAGGAGAAGAAGGAGAGGAGGGCGCCGCTATAACCGTGGTGGTGGAAGTGGTAGCTATACGACTAGGTTGAGTGTAATTTTAGAGGAGGATGAGAAGATAGAGGAGGGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

191

Amino Acids

20.8

Weight (kDa)

9.73

Isoelectric Point (pI)

70.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 181 4e-23 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014652)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g07750
malus_domestica MD10G1084300.v1.1
prunus_persica Prupe.8G107500_v2.0.a1
pyrus_communis pycom10g06630
rosa_chinensis RchiOBHm_Chr6g0262101 RchiOBHm_Chr6g0271951
rosa_laevigata RLG00000014453
rosa_multiflora Rmu_sc0002510.1_g000007 Rmu_ssc0000125.1_g000021
rosa_roxburghii Rroxscaffold_7G00207780 Rroxscaffold_7G00207820
rosa_rugosa Rorug05G0595200
rosa_samantha Rh6AG115000 Rh6BG109000 Rh6CG106300 Rh6DG096700
rosa_wichuraiana Rw6G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 448
AccB1I GGYRCC 1 cut(s) 480
AciI CCGC 4 cut(s) 336, 401, 423, 484
AclWI GGATC 1 cut(s) 204
AcoI YGGCCR 2 cut(s) 285, 402
AcsI RAATTY 1 cut(s) 77
AcuI CTGAAG 2 cut(s) 191, 471
AcyI GRCGYC 2 cut(s) 445, 481
AgsI TTSAA 2 cut(s) 59, 74
AluBI AGCT 5 cut(s) 38, 49, 70, 220, 513
AluI AGCT 5 cut(s) 38, 49, 70, 220, 513
Alw26I GTCTC 3 cut(s) 79, 103, 313
AlwI GGATC 1 cut(s) 204
Ama87I CYCGRG 1 cut(s) 82
AoxI GGCC 2 cut(s) 285, 402
ApeKI GCWGC 2 cut(s) 67, 278
ApoI RAATTY 1 cut(s) 77
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AspLEI GCGC 2 cut(s) 307, 483
AsuHPI GGTGA 1 cut(s) 428
AvaI CYCGRG 1 cut(s) 82
BanI GGYRCC 1 cut(s) 480
BbsI GAAGAC 1 cut(s) 392
BbvI GCAGC 2 cut(s) 79, 265
BccI CCATC 4 cut(s) 185, 268, 391, 428
BceAI ACGGC 1 cut(s) 272
BcoDI GTCTC 3 cut(s) 79, 103, 313
BfaI CTAG 1 cut(s) 522
BfmI CTRYAG 2 cut(s) 33, 39
BfoI RGCGCY 2 cut(s) 308, 484
BglI GCCNNNNNGGC 1 cut(s) 293
BisI GCNGC 5 cut(s) 68, 279, 402, 424, 484
BlsI GCNGC 5 cut(s) 69, 280, 403, 425, 485
BmeT110I CYCGRG 1 cut(s) 82
BmiI GGNNCC 1 cut(s) 482
BpiI GAAGAC 1 cut(s) 392
BsaBI GATNNNNATC 1 cut(s) 195
BsaHI GRCGYC 2 cut(s) 445, 481
BsaI GGTCTC 2 cut(s) 79, 103
BsaJI CCNNGG 2 cut(s) 405, 493
BsaWI WCCGGW 1 cut(s) 265
Bse1I ACTGG 3 cut(s) 351, 356, 390
Bse3DI GCAATG 1 cut(s) 107
Bse8I GATNNNNATC 1 cut(s) 195
BseDI CCNNGG 2 cut(s) 405, 493
BseGI GGATG 2 cut(s) 402, 553
BseJI GATNNNNATC 1 cut(s) 195
BseMI GCAATG 1 cut(s) 107
BseMII CTCAG 1 cut(s) 165
BseNI ACTGG 3 cut(s) 351, 356, 390
BseRI GAGGAG 3 cut(s) 475, 489, 557
BseXI GCAGC 2 cut(s) 79, 265
Bsh1285I CGRYCG 1 cut(s) 266
BshFI GGCC 2 cut(s) 287, 404
BshNI GGYRCC 1 cut(s) 480
BsiEI CGRYCG 1 cut(s) 266
BsiHKCI CYCGRG 1 cut(s) 82
BsiSI CCGG 1 cut(s) 266
BslFI GGGAC 1 cut(s) 114
BsmAI GTCTC 3 cut(s) 79, 103, 313
BsmBI CGTCTC 1 cut(s) 313
BsmFI GGGAC 1 cut(s) 114
BsnI GGCC 2 cut(s) 287, 404
Bso31I GGTCTC 2 cut(s) 79, 103
BsoBI CYCGRG 1 cut(s) 82
Bsp143I GATC 1 cut(s) 196
Bsp19I CCATGG 1 cut(s) 405
BspACI CCGC 4 cut(s) 336, 401, 423, 484
BspANI GGCC 2 cut(s) 287, 404
BspCNI CTCAG 1 cut(s) 164
BspLI GGNNCC 1 cut(s) 482
BspPI GGATC 1 cut(s) 204
BspT107I GGYRCC 1 cut(s) 480
BspTNI GGTCTC 2 cut(s) 79, 103
BsrDI GCAATG 1 cut(s) 107
BsrI ACTGG 3 cut(s) 351, 356, 390
BssECI CCNNGG 2 cut(s) 405, 493
BssMI GATC 1 cut(s) 196
BssNI GRCGYC 2 cut(s) 445, 481
BssT1I CCWWGG 1 cut(s) 405
Bst4CI ACNGT 2 cut(s) 237, 494
BstACI GRCGYC 2 cut(s) 445, 481
BstAPI GCANNNNNTGC 1 cut(s) 18
BstDEI CTNAG 1 cut(s) 151
BstDSI CCRYGG 2 cut(s) 405, 493
BstF5I GGATG 2 cut(s) 402, 553
BstH2I RGCGCY 2 cut(s) 308, 484
BstHHI GCGC 2 cut(s) 307, 483
BstKTI GATC 1 cut(s) 199
BstMAI GTCTC 3 cut(s) 79, 103, 313
BstMBI GATC 1 cut(s) 196
BstMCI CGRYCG 1 cut(s) 266
BstMWI GCNNNNNNNGC 5 cut(s) 18, 284, 293, 302, 429
BstNSI RCATGY 1 cut(s) 107
BstSFI CTRYAG 2 cut(s) 33, 39
BstV1I GCAGC 2 cut(s) 79, 265
BstV2I GAAGAC 1 cut(s) 392
BstXI CCANNNNNNTGG 1 cut(s) 397
BsuRI GGCC 2 cut(s) 287, 404
BtgI CCRYGG 2 cut(s) 405, 493
BtgZI GCGATG 1 cut(s) 447
BtsCI GGATG 2 cut(s) 402, 553
CfoI GCGC 2 cut(s) 307, 483
CviAII CATG 5 cut(s) 18, 104, 133, 160, 406
DdeI CTNAG 1 cut(s) 151
DinI GGCGCC 1 cut(s) 482
DpnI GATC 1 cut(s) 198
DpnII GATC 1 cut(s) 196
EaeI YGGCCR 2 cut(s) 285, 402
Eco130I CCWWGG 1 cut(s) 405
Eco31I GGTCTC 2 cut(s) 79, 103
Eco57I CTGAAG 2 cut(s) 191, 471
Eco88I CYCGRG 1 cut(s) 82
EcoRI GAATTC 1 cut(s) 77
EcoT14I CCWWGG 1 cut(s) 405
EcoT22I ATGCAT 2 cut(s) 109, 138
EgeI GGCGCC 1 cut(s) 482
EheI GGCGCC 1 cut(s) 482
ErhI CCWWGG 1 cut(s) 405
Esp3I CGTCTC 1 cut(s) 313
FaeI CATG 5 cut(s) 21, 107, 136, 163, 409
FaqI GGGAC 1 cut(s) 114
FatI CATG 5 cut(s) 17, 103, 132, 159, 405
FauI CCCGC 1 cut(s) 329
FauNDI CATATG 1 cut(s) 143
Fnu4HI GCNGC 5 cut(s) 68, 279, 402, 424, 484
FokI GGATG 2 cut(s) 409, 560
Fsp4HI GCNGC 5 cut(s) 68, 279, 402, 424, 484
FspBI CTAG 1 cut(s) 522
GlaI GCGC 2 cut(s) 306, 482
GluI GCNGC 5 cut(s) 68, 279, 402, 424, 484
HaeII RGCGCY 2 cut(s) 308, 484
HaeIII GGCC 2 cut(s) 287, 404
HapII CCGG 1 cut(s) 266
HhaI GCGC 2 cut(s) 307, 483
Hin1I GRCGYC 2 cut(s) 445, 481
Hin1II CATG 5 cut(s) 21, 107, 136, 163, 409
Hin6I GCGC 2 cut(s) 305, 481
HinP1I GCGC 2 cut(s) 305, 481
HinfI GANTC 4 cut(s) 23, 52, 149, 438
HpaII CCGG 1 cut(s) 266
HphI GGTGA 1 cut(s) 428
Hpy166II GTNNAC 1 cut(s) 366
Hpy188I TCNGA 3 cut(s) 154, 396, 443
Hpy188III TCNNGA 4 cut(s) 74, 82, 84, 449
Hpy8I GTNNAC 1 cut(s) 366
HpyAV CCTTC 1 cut(s) 462
HpyCH4III ACNGT 2 cut(s) 237, 494
HpyCH4IV ACGT 2 cut(s) 123, 445
HpyCH4V TGCA 5 cut(s) 12, 107, 112, 136, 569
HpyF10VI GCNNNNNNNGC 5 cut(s) 18, 284, 293, 302, 429
HpyF3I CTNAG 1 cut(s) 151
HpySE526I ACGT 2 cut(s) 123, 445
Hsp92I GRCGYC 2 cut(s) 445, 481
Hsp92II CATG 5 cut(s) 21, 107, 136, 163, 409
HspAI GCGC 2 cut(s) 305, 481
KasI GGCGCC 1 cut(s) 480
Kzo9I GATC 1 cut(s) 196
LmnI GCTCC 1 cut(s) 268
LpnPI CCDG 5 cut(s) 279, 364, 369, 403, 462
Lsp1109I GCAGC 2 cut(s) 79, 265
MaeI CTAG 1 cut(s) 522
MaeII ACGT 2 cut(s) 123, 445
MaeIII GTNAC 2 cut(s) 231, 416
MalI GATC 1 cut(s) 198
MboI GATC 1 cut(s) 196
MboII GAAGA 5 cut(s) 47, 388, 397, 477, 565
MluCI AATT 2 cut(s) 77, 534
Mly113I GGCGCC 1 cut(s) 481
MlyI GAGTC 2 cut(s) 143, 447
MmeI TCCRAC 1 cut(s) 374
Mph1103I ATGCAT 2 cut(s) 109, 138
MseI TTAA 2 cut(s) 93, 372
MspA1I CMGCKG 1 cut(s) 423
MspI CCGG 1 cut(s) 266
MwoI GCNNNNNNNGC 5 cut(s) 18, 284, 293, 302, 429
NarI GGCGCC 1 cut(s) 481
NcoI CCATGG 1 cut(s) 405
NdeI CATATG 1 cut(s) 143
NdeII GATC 1 cut(s) 196
NlaIII CATG 5 cut(s) 21, 107, 136, 163, 409
NlaIV GGNNCC 1 cut(s) 482
NmuCI GTSAC 1 cut(s) 416
NsiI ATGCAT 2 cut(s) 109, 138
NspI RCATGY 1 cut(s) 107
PaeR7I CTCGAG 1 cut(s) 82
PfeI GAWTC 2 cut(s) 23, 52
PkrI GCNGC 5 cut(s) 69, 280, 403, 425, 485
PleI GAGTC 2 cut(s) 143, 446
PluTI GGCGCC 1 cut(s) 484
PpsI GAGTC 2 cut(s) 143, 446
PspN4I GGNNCC 1 cut(s) 482
SaqAI TTAA 2 cut(s) 93, 372
SatI GCNGC 5 cut(s) 68, 279, 402, 424, 484
Sau3AI GATC 1 cut(s) 196
SchI GAGTC 2 cut(s) 143, 447
SfcI CTRYAG 2 cut(s) 33, 39
SfoI GGCGCC 1 cut(s) 482
Sfr274I CTCGAG 1 cut(s) 82
SlaI CTCGAG 1 cut(s) 82
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
Sse9I AATT 2 cut(s) 77, 534
SsiI CCGC 4 cut(s) 336, 401, 423, 484
SspDI GGCGCC 1 cut(s) 480
SspMI CTAG 1 cut(s) 522
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 2 cut(s) 237, 494
TaiI ACGT 2 cut(s) 126, 448
TaqI TCGA 2 cut(s) 83, 262
TaqII GACCGA 1 cut(s) 103
TasI AATT 2 cut(s) 77, 534
TauI GCSGC 3 cut(s) 404, 426, 486
TfiI GAWTC 2 cut(s) 23, 52
Tru1I TTAA 2 cut(s) 93, 372
Tru9I TTAA 2 cut(s) 93, 372
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 2 cut(s) 67, 278
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 2 cut(s) 130, 134
TspGWI ACGGA 1 cut(s) 337
XapI RAATTY 1 cut(s) 77
XceI RCATGY 1 cut(s) 107
XhoI CTCGAG 1 cut(s) 82
XspI CTAG 1 cut(s) 522
ZraI GACGTC 1 cut(s) 446
Zsp2I ATGCAT 2 cut(s) 109, 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.