Rroxscaffold_7G00207780

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014652
Physical Location & Seq
Forward (+)
57734636 .. 57735938
1303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00207780.1

Sequence Viewer

Length: 603 bp
ATGGGCATTTGCAGCTCGTCAACCTCCGTGGCCACAGCCAAATTGATTCTCCAAGATGGGCAGCTCCAAGAATTCTCAGACCCAGTTAGGGAATCCTACGTACTGCAGAAGATCAGCTCCCCGGGCTTTGTATGTGACGCCGACGATATGGATTTCGGAGGCTTTGTTACAGCTGTTAATGGGGAAGAAGAGCTTCTGCTGGGTCAGCTTTACTTTGTGTTGCCAGTGAGTTGGTTGAATAAGCCGCTTCGGGCAGAGGAGATGGCGGCTTTGGCTGTTAGGGCAAGTTTGGCGCTTGGCAATATGAGACGGAGTAATAGGTGTTGTTGTAAAGGAGGGGATGATCAAATTGTATTTAGTACTATTAAGAAGGATGTCAAGCCTAAGAAGGCAGCCGGCAGCGGCAGTTTTGGCGTTCATCGAGACACAGTGAGGAAAAGAAGAGGGAGAAGCGGGCGCCATGGTGGTGGTGGAAGTTATACCTCTATGGCAAAGTTGAGTGTGATTTTGGAAGACGAGGATAGGGAAGAGGGTGTCCCATCTTGGACTCCCGATATGGGAGAGTCGGAAACTATCGATGCGGCTCACATAACTATTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

200

Amino Acids

21.59

Weight (kDa)

5.68

Isoelectric Point (pI)

51.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 153 8.8e-22 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014652)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g07750
malus_domestica MD10G1084300.v1.1
prunus_persica Prupe.8G107500_v2.0.a1
pyrus_communis pycom10g06630
rosa_chinensis RchiOBHm_Chr6g0262101 RchiOBHm_Chr6g0271951
rosa_laevigata RLG00000014453
rosa_multiflora Rmu_sc0002510.1_g000007 Rmu_ssc0000125.1_g000021
rosa_roxburghii Rroxscaffold_7G00207780 Rroxscaffold_7G00207820
rosa_rugosa Rorug05G0595200
rosa_samantha Rh6AG115000 Rh6BG109000 Rh6CG106300 Rh6DG096700
rosa_wichuraiana Rw6G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 456
AciI CCGC 5 cut(s) 245, 266, 402, 453, 581
AcoI YGGCCR 1 cut(s) 30
AcsI RAATTY 1 cut(s) 71
AcyI GRCGYC 2 cut(s) 138, 457
AfaI GTAC 2 cut(s) 102, 361
AfiI CCNNNNNNNGG 2 cut(s) 88, 557
AgsI TTSAA 1 cut(s) 238
AluBI AGCT 6 cut(s) 15, 64, 117, 173, 193, 208
AluI AGCT 6 cut(s) 15, 64, 117, 173, 193, 208
Alw26I GTCTC 2 cut(s) 301, 417
Ama87I CYCGRG 1 cut(s) 121
AoxI GGCC 1 cut(s) 30
ApeKI GCWGC 4 cut(s) 12, 61, 392, 399
ApoI RAATTY 1 cut(s) 71
Asp700I GAANNNNTTC 1 cut(s) 192
AspLEI GCGC 2 cut(s) 295, 459
AsuC2I CCSGG 2 cut(s) 122, 123
AvaI CYCGRG 1 cut(s) 121
BalI TGGCCA 1 cut(s) 32
BanI GGYRCC 1 cut(s) 456
BbsI GAAGAC 1 cut(s) 519
BbvI GCAGC 4 cut(s) 24, 73, 404, 411
BccI CCATC 3 cut(s) 50, 256, 547
BclI TGATCA 1 cut(s) 343
BcnI CCSGG 2 cut(s) 122, 123
BcoDI GTCTC 2 cut(s) 301, 417
BfmI CTRYAG 1 cut(s) 104
BfoI RGCGCY 2 cut(s) 296, 460
BisI GCNGC 8 cut(s) 13, 62, 245, 267, 393, 400, 403, 582
BlsI GCNGC 8 cut(s) 14, 63, 246, 268, 394, 401, 404, 583
BmcAI AGTACT 1 cut(s) 361
Bme1390I CCNGG 2 cut(s) 122, 123
BmeT110I CYCGRG 1 cut(s) 121
BmiI GGNNCC 1 cut(s) 458
BmrFI CCNGG 2 cut(s) 122, 123
BmrI ACTGGG 1 cut(s) 77
BmsI GCATC 1 cut(s) 568
BmuI ACTGGG 1 cut(s) 77
BpiI GAAGAC 1 cut(s) 519
BpuMI CCSGG 2 cut(s) 122, 123
Bsa29I ATCGAT 1 cut(s) 576
BsaAI YACGTR 1 cut(s) 100
BsaHI GRCGYC 2 cut(s) 138, 457
BsaJI CCNNGG 4 cut(s) 27, 120, 121, 460
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 557
Bse118I RCCGGY 1 cut(s) 395
Bse1I ACTGG 2 cut(s) 83, 224
BseCI ATCGAT 1 cut(s) 576
BseDI CCNNGG 4 cut(s) 27, 120, 121, 460
BseGI GGATG 2 cut(s) 346, 379
BseLI CCNNNNNNNGG 2 cut(s) 88, 557
BseMII CTCAG 1 cut(s) 90
BseNI ACTGG 2 cut(s) 83, 224
BseRI GAGGAG 1 cut(s) 272
BseXI GCAGC 4 cut(s) 24, 73, 404, 411
BseYI CCCAGC 1 cut(s) 199
BshFI GGCC 1 cut(s) 32
BshNI GGYRCC 1 cut(s) 456
BshVI ATCGAT 1 cut(s) 576
BsiHKCI CYCGRG 1 cut(s) 121
BsiSI CCGG 2 cut(s) 122, 396
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 2 cut(s) 88, 557
BsmAI GTCTC 2 cut(s) 301, 417
BsmBI CGTCTC 1 cut(s) 301
BsmFI GGGAC 1 cut(s) 521
BsnI GGCC 1 cut(s) 32
BsoBI CYCGRG 1 cut(s) 121
Bsp143I GATC 2 cut(s) 111, 343
Bsp19I CCATGG 1 cut(s) 460
BspACI CCGC 5 cut(s) 245, 266, 402, 453, 581
BspANI GGCC 1 cut(s) 32
BspCNI CTCAG 1 cut(s) 89
BspDI ATCGAT 1 cut(s) 576
BspLI GGNNCC 1 cut(s) 458
BspMAI CTGCAG 1 cut(s) 108
BspQI GCTCTTC 1 cut(s) 183
BspT107I GGYRCC 1 cut(s) 456
BsrFI RCCGGY 1 cut(s) 395
BsrI ACTGG 2 cut(s) 83, 224
BssAI RCCGGY 1 cut(s) 395
BssECI CCNNGG 4 cut(s) 27, 120, 121, 460
BssMI GATC 2 cut(s) 111, 343
BssNI GRCGYC 2 cut(s) 138, 457
BssT1I CCWWGG 1 cut(s) 460
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 3 cut(s) 183, 436, 522
BstACI GRCGYC 2 cut(s) 138, 457
BstBAI YACGTR 1 cut(s) 100
BstC8I GCNNGC 2 cut(s) 397, 455
BstDEI CTNAG 2 cut(s) 76, 384
BstDSI CCRYGG 2 cut(s) 27, 460
BstF5I GGATG 2 cut(s) 346, 379
BstH2I RGCGCY 2 cut(s) 296, 460
BstHHI GCGC 2 cut(s) 295, 459
BstKTI GATC 2 cut(s) 114, 346
BstMAI GTCTC 2 cut(s) 301, 417
BstMBI GATC 2 cut(s) 111, 343
BstMWI GCNNNNNNNGC 7 cut(s) 12, 123, 205, 272, 281, 290, 411
BstSCI CCNGG 2 cut(s) 120, 121
BstSFI CTRYAG 1 cut(s) 104
BstSNI TACGTA 1 cut(s) 100
BstV1I GCAGC 4 cut(s) 24, 73, 404, 411
BstV2I GAAGAC 1 cut(s) 519
BstXI CCANNNNNNTGG 2 cut(s) 231, 467
Bsu15I ATCGAT 1 cut(s) 576
BsuRI GGCC 1 cut(s) 32
BsuTUI ATCGAT 1 cut(s) 576
BtgI CCRYGG 2 cut(s) 27, 460
BtsCI GGATG 2 cut(s) 346, 379
BtsIMutI CAGTG 2 cut(s) 231, 435
Cac8I GCNNGC 2 cut(s) 397, 455
CfoI GCGC 2 cut(s) 295, 459
Cfr10I RCCGGY 1 cut(s) 395
Cfr9I CCCGGG 1 cut(s) 121
ClaI ATCGAT 1 cut(s) 576
CseI GACGC 1 cut(s) 146
Csp6I GTAC 2 cut(s) 101, 360
CspCI CAANNNNNGTGG 2 cut(s) 9, 44
CviAII CATG 1 cut(s) 461
CviQI GTAC 2 cut(s) 101, 360
DdeI CTNAG 2 cut(s) 76, 384
DinI GGCGCC 1 cut(s) 458
DpnI GATC 2 cut(s) 113, 345
DpnII GATC 2 cut(s) 111, 343
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 3 cut(s) 183, 436, 522
EarI CTCTTC 3 cut(s) 183, 436, 522
Eco105I TACGTA 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 460
Eco88I CYCGRG 1 cut(s) 121
EcoRI GAATTC 1 cut(s) 71
EcoT14I CCWWGG 1 cut(s) 460
EgeI GGCGCC 1 cut(s) 458
EheI GGCGCC 1 cut(s) 458
ErhI CCWWGG 1 cut(s) 460
Esp3I CGTCTC 1 cut(s) 301
FaeI CATG 1 cut(s) 464
FaiI YATR 8 cut(s) 133, 149, 305, 462, 480, 488, 557, 590
FalI AAGNNNNNCTT 2 cut(s) 177, 209
FaqI GGGAC 1 cut(s) 521
FatI CATG 1 cut(s) 460
FauI CCCGC 1 cut(s) 446
FbaI TGATCA 1 cut(s) 343
Fnu4HI GCNGC 8 cut(s) 13, 62, 245, 267, 393, 400, 403, 582
FokI GGATG 2 cut(s) 353, 386
Fsp4HI GCNGC 8 cut(s) 13, 62, 245, 267, 393, 400, 403, 582
GlaI GCGC 2 cut(s) 294, 458
GluI GCNGC 8 cut(s) 13, 62, 245, 267, 393, 400, 403, 582
GsaI CCCAGC 1 cut(s) 203
HaeII RGCGCY 2 cut(s) 296, 460
HaeIII GGCC 1 cut(s) 32
HapII CCGG 2 cut(s) 122, 396
HgaI GACGC 1 cut(s) 146
HhaI GCGC 2 cut(s) 295, 459
Hin1I GRCGYC 2 cut(s) 138, 457
Hin1II CATG 1 cut(s) 464
Hin6I GCGC 2 cut(s) 293, 457
HinP1I GCGC 2 cut(s) 293, 457
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HinfI GANTC 4 cut(s) 46, 92, 547, 563
HpaII CCGG 2 cut(s) 122, 396
Hpy166II GTNNAC 1 cut(s) 21
Hpy188I TCNGA 3 cut(s) 79, 158, 568
Hpy188III TCNNGA 2 cut(s) 422, 551
Hpy8I GTNNAC 1 cut(s) 21
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 2 cut(s) 364, 382
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 2 cut(s) 12, 106
HpyF10VI GCNNNNNNNGC 7 cut(s) 12, 123, 205, 272, 281, 290, 411
HpyF3I CTNAG 2 cut(s) 76, 384
HpySE526I ACGT 1 cut(s) 99
Hsp92I GRCGYC 2 cut(s) 138, 457
Hsp92II CATG 1 cut(s) 464
HspAI GCGC 2 cut(s) 293, 457
KasI GGCGCC 1 cut(s) 456
KroI GCCGGC 1 cut(s) 395
KroNI GCCGGC 1 cut(s) 397
Ksp22I TGATCA 1 cut(s) 343
Kzo9I GATC 2 cut(s) 111, 343
LguI GCTCTTC 1 cut(s) 183
LmnI GCTCC 2 cut(s) 69, 122
LpnPI CCDG 5 cut(s) 96, 135, 185, 237, 409
Lsp1109I GCAGC 4 cut(s) 24, 73, 404, 411
LweI GCATC 1 cut(s) 568
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 2 cut(s) 134, 166
MalI GATC 2 cut(s) 113, 345
MboI GATC 2 cut(s) 111, 343
MboII GAAGA 6 cut(s) 121, 197, 200, 453, 524, 539
MlsI TGGCCA 1 cut(s) 32
MluCI AATT 3 cut(s) 41, 71, 348
MluNI TGGCCA 1 cut(s) 32
Mly113I GGCGCC 1 cut(s) 457
MlyI GAGTC 2 cut(s) 541, 572
MmeI TCCRAC 1 cut(s) 546
MnlI CCTC 9 cut(s) 34, 152, 250, 329, 426, 437, 493, 511, 523
Mox20I TGGCCA 1 cut(s) 32
MroNI GCCGGC 1 cut(s) 395
MroXI GAANNNNTTC 1 cut(s) 192
MscI TGGCCA 1 cut(s) 32
MseI TTAA 2 cut(s) 177, 366
MslI CAYNNNNRTG 1 cut(s) 465
Msp20I TGGCCA 1 cut(s) 32
MspA1I CMGCKG 2 cut(s) 173, 402
MspI CCGG 2 cut(s) 122, 396
MspR9I CCNGG 2 cut(s) 122, 123
MwoI GCNNNNNNNGC 7 cut(s) 12, 123, 205, 272, 281, 290, 411
NaeI GCCGGC 1 cut(s) 397
NarI GGCGCC 1 cut(s) 457
NciI CCSGG 2 cut(s) 122, 123
NcoI CCATGG 1 cut(s) 460
NdeII GATC 2 cut(s) 111, 343
NgoMIV GCCGGC 1 cut(s) 395
NlaIII CATG 1 cut(s) 464
NlaIV GGNNCC 1 cut(s) 458
NmuCI GTSAC 1 cut(s) 134
PciSI GCTCTTC 1 cut(s) 183
PdiI GCCGGC 1 cut(s) 397
PdmI GAANNNNTTC 1 cut(s) 192
PfeI GAWTC 2 cut(s) 46, 92
PkrI GCNGC 8 cut(s) 14, 63, 246, 268, 394, 401, 404, 583
PleI GAGTC 2 cut(s) 541, 571
PluTI GGCGCC 1 cut(s) 460
PpsI GAGTC 2 cut(s) 541, 571
Ppu21I YACGTR 1 cut(s) 100
PspFI CCCAGC 1 cut(s) 199
PspN4I GGNNCC 1 cut(s) 458
PstI CTGCAG 1 cut(s) 108
PvuII CAGCTG 1 cut(s) 173
RsaI GTAC 2 cut(s) 102, 361
RsaNI GTAC 2 cut(s) 101, 360
RseI CAYNNNNRTG 1 cut(s) 465
SapI GCTCTTC 1 cut(s) 183
SaqAI TTAA 2 cut(s) 177, 366
SatI GCNGC 8 cut(s) 13, 62, 245, 267, 393, 400, 403, 582
Sau3AI GATC 2 cut(s) 111, 343
ScaI AGTACT 1 cut(s) 361
SchI GAGTC 2 cut(s) 541, 572
ScrFI CCNGG 2 cut(s) 122, 123
SfaNI GCATC 1 cut(s) 568
SfcI CTRYAG 1 cut(s) 104
SfoI GGCGCC 1 cut(s) 458
SmaI CCCGGG 1 cut(s) 123
SmiMI CAYNNNNRTG 1 cut(s) 465
SnaBI TACGTA 1 cut(s) 100
Sse9I AATT 3 cut(s) 41, 71, 348
SsiI CCGC 5 cut(s) 245, 266, 402, 453, 581
SspDI GGCGCC 1 cut(s) 456
StyD4I CCNGG 2 cut(s) 120, 121
StyI CCWWGG 1 cut(s) 460
TaaI ACNGT 1 cut(s) 430
TaiI ACGT 1 cut(s) 102
TaqI TCGA 2 cut(s) 421, 576
TasI AATT 3 cut(s) 41, 71, 348
TatI WGTACW 1 cut(s) 359
TauI GCSGC 4 cut(s) 247, 269, 405, 584
TfiI GAWTC 2 cut(s) 46, 92
Tru1I TTAA 2 cut(s) 177, 366
Tru9I TTAA 2 cut(s) 177, 366
TscAI CASTG 2 cut(s) 231, 435
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 4 cut(s) 12, 61, 392, 399
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 1 cut(s) 407
TspGWI ACGGA 2 cut(s) 16, 325
TspMI CCCGGG 1 cut(s) 121
TspRI CASTG 2 cut(s) 231, 435
XapI RAATTY 1 cut(s) 71
XcmI CCANNNNNNNNNTGG 1 cut(s) 467
XmaI CCCGGG 1 cut(s) 121
XmnI GAANNNNTTC 1 cut(s) 192
ZrmI AGTACT 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.