Prupe.8G107500_v2.0.a1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0014652
Physical Location & Seq
Reverse (-)
13834252 .. 13834836
585 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G107500.1

Sequence Viewer

Length: 585 bp
ATGGGCATTTGCAGCTCAAGCGACTCAGCCTCTGTGTCTGTTGTCACAGCCAAGGTGATTCTCCAGGACGGGCAGCTTCAAGAATTCTCAAACCCAGTTAAGGTCTCGCACGTGCTTCAAAAGAGTCCCACGTGCTTCATATGCGACTCCGACGACATGGATTTTGGAGGCTTTGTTAGGGCCGTGAATGGGGACGAAGAGCTTCAGCTCGGTCAGCTTTATTTTGTGTTGCCGTTGAGCTGGCTGAGCAGGCCGCTTCGAGCCGAGCAGATGGCTGCTTTGGCTGTTAGGGCAAGCTTGGCACTTGGAAATACGAGGCGGAGGAAGCGATGTTTTGGGCGTGGACGTAAAAGAGTCAATCAAGTTGTGTTTACTATTAAGAAGAAGGATGAGCCTAGCCCGATGGTGGCCATGGGCAGTTATGGTGGCGGCCCGAGCGTCGCTAGAAGTGGAGAAGTTGTGAGGAAAAGGAGAGGGAGAGGCCGCGGGCGGCGCGGTCATGGCGGTGGTAATGGAGGAGGTGGTGGCTTTGTGACAAGGTTGAGTGTGATTTTAGAGGATGATGATATGATGGAGGGGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

195

Amino Acids

20.88

Weight (kDa)

9.96

Isoelectric Point (pI)

56.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014652)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g07750
malus_domestica MD10G1084300.v1.1
prunus_persica Prupe.8G107500_v2.0.a1
pyrus_communis pycom10g06630
rosa_chinensis RchiOBHm_Chr6g0262101 RchiOBHm_Chr6g0271951
rosa_laevigata RLG00000014453
rosa_multiflora Rmu_sc0002510.1_g000007 Rmu_ssc0000125.1_g000021
rosa_roxburghii Rroxscaffold_7G00207780 Rroxscaffold_7G00207820
rosa_rugosa Rorug05G0595200
rosa_samantha Rh6AG115000 Rh6BG109000 Rh6CG106300 Rh6DG096700
rosa_wichuraiana Rw6G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 486, 495
AciI CCGC 8 cut(s) 254, 319, 429, 484, 486, 490, 495, 504
AcoI YGGCCR 1 cut(s) 408
AcsI RAATTY 1 cut(s) 83
AcuI CTGAAG 1 cut(s) 188
AcvI CACGTG 2 cut(s) 112, 132
AfiI CCNNNNNNNGG 3 cut(s) 100, 189, 406
AgsI TTSAA 2 cut(s) 80, 119
AjnI CCWGG 1 cut(s) 63
AluBI AGCT 7 cut(s) 15, 76, 202, 208, 217, 240, 297
AluI AGCT 7 cut(s) 15, 76, 202, 208, 217, 240, 297
Alw26I GTCTC 1 cut(s) 109
AlwNI CAGNNNCTG 1 cut(s) 32
Ama87I CYCGRG 1 cut(s) 433
AoxI GGCC 5 cut(s) 180, 251, 408, 430, 481
ApeKI GCWGC 3 cut(s) 12, 73, 275
ApoI RAATTY 1 cut(s) 83
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
Asp700I GAANNNNTTC 1 cut(s) 201
AspLEI GCGC 1 cut(s) 495
AspS9I GGNCC 2 cut(s) 180, 431
AsuHPI GGTGA 1 cut(s) 67
AvaI CYCGRG 1 cut(s) 433
BalI TGGCCA 1 cut(s) 410
BbrPI CACGTG 2 cut(s) 112, 132
BbvI GCAGC 3 cut(s) 24, 85, 262
BccI CCATC 3 cut(s) 265, 397, 565
BceAI ACGGC 2 cut(s) 167, 217
BciT130I CCWGG 1 cut(s) 65
BcoDI GTCTC 1 cut(s) 109
BfaI CTAG 2 cut(s) 396, 444
BisI GCNGC 7 cut(s) 13, 74, 254, 276, 430, 484, 491
BlpI GCTNAGC 1 cut(s) 245
BlsI GCNGC 7 cut(s) 14, 75, 255, 277, 431, 485, 492
Bme1390I CCNGG 1 cut(s) 65
BmeT110I CYCGRG 1 cut(s) 433
BmgT120I GGNCC 2 cut(s) 180, 431
BmrFI CCNGG 1 cut(s) 65
BmrI ACTGGG 1 cut(s) 89
BmuI ACTGGG 1 cut(s) 89
BpmI CTGGAG 1 cut(s) 47
Bpu1102I GCTNAGC 1 cut(s) 245
BsaAI YACGTR 2 cut(s) 112, 132
BsaI GGTCTC 1 cut(s) 109
BsaJI CCNNGG 3 cut(s) 51, 411, 484
BsaXI ACNNNNNCTCC 4 cut(s) 159, 189, 444, 474
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 189, 406
Bse1I ACTGG 1 cut(s) 95
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 3 cut(s) 51, 411, 484
BseGI GGATG 2 cut(s) 394, 565
BseLI CCNNNNNNNGG 3 cut(s) 100, 189, 406
BseMII CTCAG 2 cut(s) 39, 236
BseNI ACTGG 1 cut(s) 95
BseRI GAGGAG 1 cut(s) 531
BseXI GCAGC 3 cut(s) 24, 85, 262
Bsh1236I CGCG 2 cut(s) 486, 495
BshFI GGCC 5 cut(s) 182, 253, 410, 432, 483
BsiHKCI CYCGRG 1 cut(s) 433
BslFI GGGAC 2 cut(s) 111, 206
BslI CCNNNNNNNGG 3 cut(s) 100, 189, 406
BsmAI GTCTC 1 cut(s) 109
BsmFI GGGAC 2 cut(s) 111, 206
BsnI GGCC 5 cut(s) 182, 253, 410, 432, 483
Bso31I GGTCTC 1 cut(s) 109
BsoBI CYCGRG 1 cut(s) 433
Bsp1720I GCTNAGC 1 cut(s) 245
Bsp19I CCATGG 1 cut(s) 411
BspACI CCGC 8 cut(s) 254, 319, 429, 484, 486, 490, 495, 504
BspANI GGCC 5 cut(s) 182, 253, 410, 432, 483
BspCNI CTCAG 2 cut(s) 38, 237
BspFNI CGCG 2 cut(s) 486, 495
BspQI GCTCTTC 1 cut(s) 192
BspTNI GGTCTC 1 cut(s) 109
BsrI ACTGG 1 cut(s) 95
BssECI CCNNGG 3 cut(s) 51, 411, 484
BssT1I CCWWGG 2 cut(s) 51, 411
Bst2UI CCWGG 1 cut(s) 65
Bst6I CTCTTC 1 cut(s) 192
BstBAI YACGTR 2 cut(s) 112, 132
BstC8I GCNNGC 4 cut(s) 242, 251, 295, 488
BstDEI CTNAG 2 cut(s) 25, 245
BstDSI CCRYGG 2 cut(s) 411, 484
BstF5I GGATG 2 cut(s) 394, 565
BstFNI CGCG 2 cut(s) 486, 495
BstHHI GCGC 1 cut(s) 495
BstMAI GTCTC 1 cut(s) 109
BstNI CCWGG 1 cut(s) 65
BstSCI CCNGG 1 cut(s) 63
BstUI CGCG 2 cut(s) 486, 495
BstV1I GCAGC 3 cut(s) 24, 85, 262
BsuRI GGCC 5 cut(s) 182, 253, 410, 432, 483
BtgI CCRYGG 2 cut(s) 411, 484
BtgZI GCGATG 1 cut(s) 343
BtsCI GGATG 2 cut(s) 394, 565
Cac8I GCNNGC 4 cut(s) 242, 251, 295, 488
CaiI CAGNNNCTG 1 cut(s) 32
CfoI GCGC 1 cut(s) 495
Cfr13I GGNCC 2 cut(s) 180, 431
Cfr42I CCGCGG 1 cut(s) 487
CseI GACGC 1 cut(s) 427
CviAII CATG 4 cut(s) 157, 412, 500, 582
DdeI CTNAG 2 cut(s) 25, 245
EaeI YGGCCR 1 cut(s) 408
Eam1104I CTCTTC 1 cut(s) 192
EarI CTCTTC 1 cut(s) 192
EciI GGCGGA 1 cut(s) 334
Eco130I CCWWGG 2 cut(s) 51, 411
Eco31I GGTCTC 1 cut(s) 109
Eco57I CTGAAG 1 cut(s) 188
Eco72I CACGTG 2 cut(s) 112, 132
Eco88I CYCGRG 1 cut(s) 433
EcoRI GAATTC 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 63
EcoT14I CCWWGG 2 cut(s) 51, 411
ErhI CCWWGG 2 cut(s) 51, 411
FaeI CATG 4 cut(s) 160, 415, 503, 585
FaiI YATR 8 cut(s) 140, 142, 158, 413, 423, 501, 569, 583
FaqI GGGAC 2 cut(s) 111, 206
FatI CATG 4 cut(s) 156, 411, 499, 581
FauI CCCGC 1 cut(s) 479
FauNDI CATATG 1 cut(s) 140
Fnu4HI GCNGC 7 cut(s) 13, 74, 254, 276, 430, 484, 491
FokI GGATG 2 cut(s) 401, 572
Fsp4HI GCNGC 7 cut(s) 13, 74, 254, 276, 430, 484, 491
FspBI CTAG 2 cut(s) 396, 444
GlaI GCGC 1 cut(s) 494
GluI GCNGC 7 cut(s) 13, 74, 254, 276, 430, 484, 491
GsuI CTGGAG 1 cut(s) 47
HaeIII GGCC 5 cut(s) 182, 253, 410, 432, 483
HgaI GACGC 1 cut(s) 427
HhaI GCGC 1 cut(s) 495
Hin1II CATG 4 cut(s) 160, 415, 503, 585
Hin6I GCGC 1 cut(s) 493
HinP1I GCGC 1 cut(s) 493
HindIII AAGCTT 1 cut(s) 295
HinfI GANTC 5 cut(s) 23, 58, 124, 146, 354
HphI GGTGA 1 cut(s) 67
Hpy166II GTNNAC 2 cut(s) 344, 372
Hpy188I TCNGA 1 cut(s) 151
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 2 cut(s) 344, 372
Hpy99I CGWCG 2 cut(s) 155, 443
HpyAV CCTTC 1 cut(s) 379
HpyCH4IV ACGT 3 cut(s) 111, 131, 346
HpyCH4V TGCA 1 cut(s) 12
HpyF3I CTNAG 2 cut(s) 25, 245
HpySE526I ACGT 3 cut(s) 111, 131, 346
Hsp92II CATG 4 cut(s) 160, 415, 503, 585
HspAI GCGC 1 cut(s) 493
KspI CCGCGG 1 cut(s) 487
LguI GCTCTTC 1 cut(s) 192
LpnPI CCDG 5 cut(s) 50, 77, 108, 226, 235
Lsp1109I GCAGC 3 cut(s) 24, 85, 262
MaeI CTAG 2 cut(s) 396, 444
MaeII ACGT 3 cut(s) 111, 131, 346
MaeIII GTNAC 2 cut(s) 43, 532
MboII GAAGA 2 cut(s) 209, 394
MlsI TGGCCA 1 cut(s) 410
MluCI AATT 1 cut(s) 83
MluNI TGGCCA 1 cut(s) 410
MlyI GAGTC 4 cut(s) 17, 133, 140, 363
MmeI TCCRAC 1 cut(s) 174
Mox20I TGGCCA 1 cut(s) 410
MroXI GAANNNNTTC 1 cut(s) 201
MscI TGGCCA 1 cut(s) 410
MseI TTAA 2 cut(s) 99, 378
MslI CAYNNNNRTG 1 cut(s) 504
Msp20I TGGCCA 1 cut(s) 410
MspA1I CMGCKG 1 cut(s) 486
MspR9I CCNGG 1 cut(s) 65
MvaI CCWGG 1 cut(s) 65
MvnI CGCG 2 cut(s) 486, 495
NcoI CCATGG 1 cut(s) 411
NdeI CATATG 1 cut(s) 140
NlaIII CATG 4 cut(s) 160, 415, 503, 585
NmeAIII GCCGAG 1 cut(s) 289
NmuCI GTSAC 2 cut(s) 43, 532
PciSI GCTCTTC 1 cut(s) 192
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 1 cut(s) 58
PfoI TCCNGGA 1 cut(s) 63
PkrI GCNGC 7 cut(s) 14, 75, 255, 277, 431, 485, 492
PleI GAGTC 4 cut(s) 17, 132, 140, 362
PmaCI CACGTG 2 cut(s) 112, 132
PmlI CACGTG 2 cut(s) 112, 132
PpsI GAGTC 4 cut(s) 17, 132, 140, 362
Ppu21I YACGTR 2 cut(s) 112, 132
Psp6I CCWGG 1 cut(s) 63
PspCI CACGTG 2 cut(s) 112, 132
PspGI CCWGG 1 cut(s) 63
PspPI GGNCC 2 cut(s) 180, 431
PstNI CAGNNNCTG 1 cut(s) 32
RseI CAYNNNNRTG 1 cut(s) 504
SacII CCGCGG 1 cut(s) 487
SapI GCTCTTC 1 cut(s) 192
SaqAI TTAA 2 cut(s) 99, 378
SatI GCNGC 7 cut(s) 13, 74, 254, 276, 430, 484, 491
Sau96I GGNCC 2 cut(s) 180, 431
SchI GAGTC 4 cut(s) 17, 133, 140, 363
ScrFI CCNGG 1 cut(s) 65
Sfr303I CCGCGG 1 cut(s) 487
SgrBI CCGCGG 1 cut(s) 487
SmiMI CAYNNNNRTG 1 cut(s) 504
SmlI CTYRAG 1 cut(s) 16
SmoI CTYRAG 1 cut(s) 16
Sse9I AATT 1 cut(s) 83
SsiI CCGC 8 cut(s) 254, 319, 429, 484, 486, 490, 495, 504
SspMI CTAG 2 cut(s) 396, 444
StyD4I CCNGG 1 cut(s) 63
StyI CCWWGG 2 cut(s) 51, 411
TaiI ACGT 3 cut(s) 114, 134, 349
TaqI TCGA 1 cut(s) 259
TaqII GACCGA 1 cut(s) 200
TasI AATT 1 cut(s) 83
TauI GCSGC 4 cut(s) 256, 432, 486, 493
TfiI GAWTC 1 cut(s) 58
Tru1I TTAA 2 cut(s) 99, 378
Tru9I TTAA 2 cut(s) 99, 378
TseFI GTSAC 2 cut(s) 43, 532
TseI GCWGC 3 cut(s) 12, 73, 275
Tsp45I GTSAC 2 cut(s) 43, 532
TspDTI ATGAA 1 cut(s) 127
XapI RAATTY 1 cut(s) 83
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 2 cut(s) 396, 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.