RLG00000014453

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0014652
Physical Location & Seq
Reverse (-)
53329631 .. 53330364
734 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014453

Sequence Viewer

Length: 588 bp
ATGGCGATCAATCCCAAGTTGCAAGAAAAGTTAACACAAATAAACTCGTCAACCTCCGTGGCCACAGCCAAATTGATTCTCCAAGATGGGCAGCTCCAAGAATTCTCAGACCCAGTTAGGGCATCCTACGCACTGCAGAAGATCAGCTCCCCGGGCTTTGTATGTGACGCCGACGACATGGATTTAGGAGGCTTTGTTACAGCTGTTAATGGGGAAGAAGATCTTCAGCTGGGTCAGCTTTACTTTGTGTTGCCGGTGAGTTGGTTGAATAAGCCGCTTCGGGCAGAGGAGATGGCGGCTTTGGCTGTTAGGGCAAGTTTGGCTCTTGGCAATATGAGACGGAGTAATAGGTGTTGTTGTAAACGAGGGGATGATCAAATTGTATGTAGTACTATTAAGAAGGATGTCAAACCTAAGATGGCAGCCGGCAGCGGCAGTTTTGGCGTTCATCGAGACACAGTGAGGAAAAGAAGAGGGAGAAGCGGACGCCATGGTGGTGGTGGAAGTTATACCTCTATGGCAAAGTTGAGTGTGGTTTTGGAAGACGAGGATAGGGAAGAGGGTGGTGAGATTTGGGTTCTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

196

Amino Acids

21.22

Weight (kDa)

8.27

Isoelectric Point (pI)

56.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 18 - 124 1e-20 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014652)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g07750
malus_domestica MD10G1084300.v1.1
prunus_persica Prupe.8G107500_v2.0.a1
pyrus_communis pycom10g06630
rosa_chinensis RchiOBHm_Chr6g0262101 RchiOBHm_Chr6g0271951
rosa_laevigata RLG00000014453
rosa_multiflora Rmu_sc0002510.1_g000007 Rmu_ssc0000125.1_g000021
rosa_roxburghii Rroxscaffold_7G00207780 Rroxscaffold_7G00207820
rosa_rugosa Rorug05G0595200
rosa_samantha Rh6AG115000 Rh6BG109000 Rh6CG106300 Rh6DG096700
rosa_wichuraiana Rw6G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 275, 296, 432, 483
AcoI YGGCCR 1 cut(s) 60
AcsI RAATTY 1 cut(s) 101
AcuI CTGAAG 1 cut(s) 209
AcyI GRCGYC 2 cut(s) 168, 487
AfaI GTAC 1 cut(s) 391
AfiI CCNNNNNNNGG 1 cut(s) 118
AgsI TTSAA 1 cut(s) 268
AluBI AGCT 5 cut(s) 94, 147, 203, 229, 238
AluI AGCT 5 cut(s) 94, 147, 203, 229, 238
Alw26I GTCTC 2 cut(s) 331, 447
Ama87I CYCGRG 1 cut(s) 151
AoxI GGCC 1 cut(s) 60
ApeKI GCWGC 3 cut(s) 91, 422, 429
ApoI RAATTY 1 cut(s) 101
Asp700I GAANNNNTTC 1 cut(s) 222
AsuC2I CCSGG 2 cut(s) 152, 153
AsuHPI GGTGA 2 cut(s) 268, 578
AvaI CYCGRG 1 cut(s) 151
BalI TGGCCA 1 cut(s) 62
BbsI GAAGAC 1 cut(s) 549
BbvI GCAGC 3 cut(s) 103, 434, 441
BccI CCATC 3 cut(s) 80, 286, 412
BclI TGATCA 1 cut(s) 373
BcnI CCSGG 2 cut(s) 152, 153
BcoDI GTCTC 2 cut(s) 331, 447
BfmI CTRYAG 1 cut(s) 134
BglII AGATCT 1 cut(s) 220
BisI GCNGC 6 cut(s) 92, 275, 297, 423, 430, 433
BlsI GCNGC 6 cut(s) 93, 276, 298, 424, 431, 434
BmcAI AGTACT 1 cut(s) 391
Bme1390I CCNGG 2 cut(s) 152, 153
BmeT110I CYCGRG 1 cut(s) 151
BmrFI CCNGG 2 cut(s) 152, 153
BmrI ACTGGG 1 cut(s) 107
BmsI GCATC 1 cut(s) 131
BmuI ACTGGG 1 cut(s) 107
BpiI GAAGAC 1 cut(s) 549
BpuMI CCSGG 2 cut(s) 152, 153
BsaHI GRCGYC 2 cut(s) 168, 487
BsaJI CCNNGG 4 cut(s) 57, 150, 151, 490
BsaXI ACNNNNNCTCC 2 cut(s) 180, 210
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse118I RCCGGY 2 cut(s) 253, 425
Bse1I ACTGG 1 cut(s) 113
BseDI CCNNGG 4 cut(s) 57, 150, 151, 490
BseGI GGATG 3 cut(s) 122, 376, 409
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMII CTCAG 1 cut(s) 120
BseNI ACTGG 1 cut(s) 113
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 3 cut(s) 103, 434, 441
BseYI CCCAGC 1 cut(s) 229
BshFI GGCC 1 cut(s) 62
BsiHKCI CYCGRG 1 cut(s) 151
BsiSI CCGG 3 cut(s) 152, 254, 426
BslI CCNNNNNNNGG 1 cut(s) 118
BsmAI GTCTC 2 cut(s) 331, 447
BsmBI CGTCTC 1 cut(s) 331
BsnI GGCC 1 cut(s) 62
BsoBI CYCGRG 1 cut(s) 151
Bsp143I GATC 4 cut(s) 6, 141, 220, 373
Bsp19I CCATGG 1 cut(s) 490
BspACI CCGC 4 cut(s) 275, 296, 432, 483
BspANI GGCC 1 cut(s) 62
BspCNI CTCAG 1 cut(s) 119
BspMAI CTGCAG 1 cut(s) 138
BsrFI RCCGGY 2 cut(s) 253, 425
BsrI ACTGG 1 cut(s) 113
BssAI RCCGGY 2 cut(s) 253, 425
BssECI CCNNGG 4 cut(s) 57, 150, 151, 490
BssMI GATC 4 cut(s) 6, 141, 220, 373
BssNI GRCGYC 2 cut(s) 168, 487
BssT1I CCWWGG 1 cut(s) 490
Bst4CI ACNGT 1 cut(s) 460
Bst6I CTCTTC 2 cut(s) 466, 552
BstACI GRCGYC 2 cut(s) 168, 487
BstC8I GCNNGC 1 cut(s) 427
BstDEI CTNAG 2 cut(s) 106, 414
BstDSI CCRYGG 2 cut(s) 57, 490
BstF5I GGATG 3 cut(s) 122, 376, 409
BstKTI GATC 4 cut(s) 9, 144, 223, 376
BstMAI GTCTC 2 cut(s) 331, 447
BstMBI GATC 4 cut(s) 6, 141, 220, 373
BstMWI GCNNNNNNNGC 7 cut(s) 128, 153, 235, 302, 311, 320, 441
BstSCI CCNGG 2 cut(s) 150, 151
BstSFI CTRYAG 1 cut(s) 134
BstV1I GCAGC 3 cut(s) 103, 434, 441
BstV2I GAAGAC 1 cut(s) 549
BstX2I RGATCY 1 cut(s) 220
BstXI CCANNNNNNTGG 1 cut(s) 497
BstYI RGATCY 1 cut(s) 220
BsuRI GGCC 1 cut(s) 62
BtgI CCRYGG 2 cut(s) 57, 490
BtsCI GGATG 3 cut(s) 122, 376, 409
BtsI GCAGTG 1 cut(s) 131
BtsIMutI CAGTG 2 cut(s) 131, 465
Cac8I GCNNGC 1 cut(s) 427
Cfr10I RCCGGY 2 cut(s) 253, 425
Cfr9I CCCGGG 1 cut(s) 151
CseI GACGC 2 cut(s) 176, 495
Csp6I GTAC 1 cut(s) 390
CspCI CAANNNNNGTGG 2 cut(s) 39, 74
CviAII CATG 2 cut(s) 178, 491
CviQI GTAC 1 cut(s) 390
DdeI CTNAG 2 cut(s) 106, 414
DpnI GATC 4 cut(s) 8, 143, 222, 375
DpnII GATC 4 cut(s) 6, 141, 220, 373
EaeI YGGCCR 1 cut(s) 60
Eam1104I CTCTTC 2 cut(s) 466, 552
EarI CTCTTC 2 cut(s) 466, 552
Eco130I CCWWGG 1 cut(s) 490
Eco57I CTGAAG 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 151
EcoRI GAATTC 1 cut(s) 101
EcoT14I CCWWGG 1 cut(s) 490
ErhI CCWWGG 1 cut(s) 490
Esp3I CGTCTC 1 cut(s) 331
FaeI CATG 2 cut(s) 181, 494
FaiI YATR 7 cut(s) 163, 179, 335, 385, 492, 510, 518
FalI AAGNNNNNCTT 2 cut(s) 207, 239
FatI CATG 2 cut(s) 177, 490
FbaI TGATCA 1 cut(s) 373
Fnu4HI GCNGC 6 cut(s) 92, 275, 297, 423, 430, 433
FokI GGATG 3 cut(s) 109, 383, 416
Fsp4HI GCNGC 6 cut(s) 92, 275, 297, 423, 430, 433
GluI GCNGC 6 cut(s) 92, 275, 297, 423, 430, 433
GsaI CCCAGC 1 cut(s) 233
HaeIII GGCC 1 cut(s) 62
HapII CCGG 3 cut(s) 152, 254, 426
HgaI GACGC 2 cut(s) 176, 495
Hin1I GRCGYC 2 cut(s) 168, 487
Hin1II CATG 2 cut(s) 181, 494
HincII GTYRAC 2 cut(s) 33, 51
HindII GTYRAC 2 cut(s) 33, 51
HinfI GANTC 1 cut(s) 76
HpaI GTTAAC 1 cut(s) 33
HpaII CCGG 3 cut(s) 152, 254, 426
HphI GGTGA 2 cut(s) 268, 578
Hpy166II GTNNAC 3 cut(s) 33, 51, 362
Hpy188I TCNGA 1 cut(s) 109
Hpy188III TCNNGA 1 cut(s) 452
Hpy8I GTNNAC 3 cut(s) 33, 51, 362
Hpy99I CGWCG 1 cut(s) 176
HpyAV CCTTC 1 cut(s) 394
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4V TGCA 3 cut(s) 22, 136, 583
HpyF10VI GCNNNNNNNGC 7 cut(s) 128, 153, 235, 302, 311, 320, 441
HpyF3I CTNAG 2 cut(s) 106, 414
Hsp92I GRCGYC 2 cut(s) 168, 487
Hsp92II CATG 2 cut(s) 181, 494
KroI GCCGGC 1 cut(s) 425
KroNI GCCGGC 1 cut(s) 427
Ksp22I TGATCA 1 cut(s) 373
KspAI GTTAAC 1 cut(s) 33
Kzo9I GATC 4 cut(s) 6, 141, 220, 373
LmnI GCTCC 2 cut(s) 99, 152
LpnPI CCDG 5 cut(s) 126, 165, 215, 267, 439
Lsp1109I GCAGC 3 cut(s) 103, 434, 441
LweI GCATC 1 cut(s) 131
MaeIII GTNAC 2 cut(s) 164, 196
MalI GATC 4 cut(s) 8, 143, 222, 375
MboI GATC 4 cut(s) 6, 141, 220, 373
MboII GAAGA 7 cut(s) 151, 215, 227, 230, 483, 554, 569
MflI RGATCY 1 cut(s) 220
MlsI TGGCCA 1 cut(s) 62
MluCI AATT 3 cut(s) 71, 101, 378
MluNI TGGCCA 1 cut(s) 62
MnlI CCTC 9 cut(s) 64, 182, 280, 359, 456, 467, 523, 541, 553
Mox20I TGGCCA 1 cut(s) 62
MroNI GCCGGC 1 cut(s) 425
MroXI GAANNNNTTC 1 cut(s) 222
MscI TGGCCA 1 cut(s) 62
MseI TTAA 3 cut(s) 32, 207, 396
MslI CAYNNNNRTG 1 cut(s) 495
Msp20I TGGCCA 1 cut(s) 62
MspA1I CMGCKG 3 cut(s) 203, 229, 432
MspI CCGG 3 cut(s) 152, 254, 426
MspR9I CCNGG 2 cut(s) 152, 153
MwoI GCNNNNNNNGC 7 cut(s) 128, 153, 235, 302, 311, 320, 441
NaeI GCCGGC 1 cut(s) 427
NciI CCSGG 2 cut(s) 152, 153
NcoI CCATGG 1 cut(s) 490
NdeII GATC 4 cut(s) 6, 141, 220, 373
NgoMIV GCCGGC 1 cut(s) 425
NlaIII CATG 2 cut(s) 181, 494
NmuCI GTSAC 1 cut(s) 164
PdiI GCCGGC 1 cut(s) 427
PdmI GAANNNNTTC 1 cut(s) 222
PfeI GAWTC 1 cut(s) 76
PkrI GCNGC 6 cut(s) 93, 276, 298, 424, 431, 434
PspFI CCCAGC 1 cut(s) 229
PstI CTGCAG 1 cut(s) 138
PsuI RGATCY 1 cut(s) 220
PvuII CAGCTG 2 cut(s) 203, 229
RsaI GTAC 1 cut(s) 391
RsaNI GTAC 1 cut(s) 390
RseI CAYNNNNRTG 1 cut(s) 495
SaqAI TTAA 3 cut(s) 32, 207, 396
SatI GCNGC 6 cut(s) 92, 275, 297, 423, 430, 433
Sau3AI GATC 4 cut(s) 6, 141, 220, 373
ScaI AGTACT 1 cut(s) 391
ScrFI CCNGG 2 cut(s) 152, 153
SetI ASST 9 cut(s) 56, 96, 149, 205, 231, 240, 353, 415, 515
SfaNI GCATC 1 cut(s) 131
SfcI CTRYAG 1 cut(s) 134
SmaI CCCGGG 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 495
Sse9I AATT 3 cut(s) 71, 101, 378
SsiI CCGC 4 cut(s) 275, 296, 432, 483
StyD4I CCNGG 2 cut(s) 150, 151
StyI CCWWGG 1 cut(s) 490
TaaI ACNGT 1 cut(s) 460
TaqI TCGA 1 cut(s) 451
TasI AATT 3 cut(s) 71, 101, 378
TatI WGTACW 1 cut(s) 389
TauI GCSGC 3 cut(s) 277, 299, 435
TfiI GAWTC 1 cut(s) 76
Tru1I TTAA 3 cut(s) 32, 207, 396
Tru9I TTAA 3 cut(s) 32, 207, 396
TscAI CASTG 2 cut(s) 138, 465
TseFI GTSAC 1 cut(s) 164
TseI GCWGC 3 cut(s) 91, 422, 429
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 1 cut(s) 437
TspGWI ACGGA 2 cut(s) 46, 355
TspMI CCCGGG 1 cut(s) 151
TspRI CASTG 2 cut(s) 138, 465
XapI RAATTY 1 cut(s) 101
XcmI CCANNNNNNNNNTGG 1 cut(s) 497
XmaI CCCGGG 1 cut(s) 151
XmnI GAANNNNTTC 1 cut(s) 222
ZrmI AGTACT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.