RchiOBHm_Chr6g0271951

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0014652
Physical Location & Seq
Forward (+)
31983659 .. 31984437
779 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24394

Sequence Viewer

Length: 564 bp
ATGACTCATTATCTAAATGGGCATTTGCAGCTCGTCAACCTCCGTGGCCACAGCCGAACTGATTCTCCAAGACGGGCAGCTCCAAGAATTCTCAGACCCAGTTACGTACTCCAGAAGATCAGCTCCCCGGGCTTTGTATGTGACGCCGACGACATGGATGTCGGAGGCTTTGTTACAGCTGTTAATGGGGAAGAAGAGCTTCAGCTGGGTCAGCTTTACTTTGTGTTGCCGGTGAGTTGGTTGAATAAGCCGCTTCAGGCAGATGAGATGGCGGCTTTGGCTGTTAGGGCAAGTTTGGCGCTTGGCAATATGAGACGGAGTAATAGGTGTTGTTGTAAAGGAGGGGATGATCAAATTGTATGTAGTACTATTAAGAAGGATGTCAAGCCTAAGATGGCAGCCGGCAGCGGCAGTTTTGGCGTTCATCGAGACACAGTGAGGAAAAGAAGAGGGAGAAGCGGACGCCATGGTGGTGGTGGAAGTTATACCTCTATGGCAAAGTTGAGTGTGATTTTGGAAGACGAGGATAGGGAAGAGGGTGGTGAGATTTGGGTTCTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

187

Amino Acids

20.47

Weight (kDa)

9.06

Isoelectric Point (pI)

60.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 44 - 115 9.7e-14 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014652)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g07750
malus_domestica MD10G1084300.v1.1
prunus_persica Prupe.8G107500_v2.0.a1
pyrus_communis pycom10g06630
rosa_chinensis RchiOBHm_Chr6g0262101 RchiOBHm_Chr6g0271951
rosa_laevigata RLG00000014453
rosa_multiflora Rmu_sc0002510.1_g000007 Rmu_ssc0000125.1_g000021
rosa_roxburghii Rroxscaffold_7G00207780 Rroxscaffold_7G00207820
rosa_rugosa Rorug05G0595200
rosa_samantha Rh6AG115000 Rh6BG109000 Rh6CG106300 Rh6DG096700
rosa_wichuraiana Rw6G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 158
AciI CCGC 4 cut(s) 251, 272, 408, 459
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 1 cut(s) 87
AcuI CTGAAG 2 cut(s) 185, 239
AcyI GRCGYC 2 cut(s) 144, 463
AfaI GTAC 2 cut(s) 108, 367
AgsI TTSAA 1 cut(s) 244
AloI GAACNNNNNNTCC 2 cut(s) 49, 81
AluBI AGCT 7 cut(s) 31, 80, 123, 179, 199, 205, 214
AluI AGCT 7 cut(s) 31, 80, 123, 179, 199, 205, 214
Alw26I GTCTC 2 cut(s) 307, 423
Ama87I CYCGRG 1 cut(s) 127
AoxI GGCC 1 cut(s) 46
ApeKI GCWGC 4 cut(s) 28, 77, 398, 405
ApoI RAATTY 1 cut(s) 87
Asp700I GAANNNNTTC 2 cut(s) 61, 198
AspLEI GCGC 1 cut(s) 301
AsuC2I CCSGG 2 cut(s) 128, 129
AsuHPI GGTGA 2 cut(s) 244, 554
AvaI CYCGRG 1 cut(s) 127
BalI TGGCCA 1 cut(s) 48
BbsI GAAGAC 1 cut(s) 525
BbvI GCAGC 4 cut(s) 40, 89, 410, 417
BccI CCATC 2 cut(s) 262, 388
BclI TGATCA 1 cut(s) 349
BcnI CCSGG 2 cut(s) 128, 129
BcoDI GTCTC 2 cut(s) 307, 423
BfoI RGCGCY 1 cut(s) 302
BisI GCNGC 7 cut(s) 29, 78, 251, 273, 399, 406, 409
BlsI GCNGC 7 cut(s) 30, 79, 252, 274, 400, 407, 410
BmcAI AGTACT 1 cut(s) 367
Bme1390I CCNGG 2 cut(s) 128, 129
BmeT110I CYCGRG 1 cut(s) 127
BmrFI CCNGG 2 cut(s) 128, 129
BmrI ACTGGG 1 cut(s) 93
BmuI ACTGGG 1 cut(s) 93
BpiI GAAGAC 1 cut(s) 525
BpmI CTGGAG 1 cut(s) 95
BpuMI CCSGG 2 cut(s) 128, 129
BsaAI YACGTR 1 cut(s) 106
BsaHI GRCGYC 2 cut(s) 144, 463
BsaJI CCNNGG 4 cut(s) 43, 126, 127, 466
BsaXI ACNNNNNCTCC 4 cut(s) 49, 79, 156, 186
Bse118I RCCGGY 2 cut(s) 229, 401
Bse1I ACTGG 1 cut(s) 99
BseDI CCNNGG 4 cut(s) 43, 126, 127, 466
BseGI GGATG 3 cut(s) 163, 352, 385
BseMII CTCAG 1 cut(s) 106
BseNI ACTGG 1 cut(s) 99
BseXI GCAGC 4 cut(s) 40, 89, 410, 417
BseYI CCCAGC 1 cut(s) 205
BshFI GGCC 1 cut(s) 48
BsiHKCI CYCGRG 1 cut(s) 127
BsiSI CCGG 3 cut(s) 128, 230, 402
BsmAI GTCTC 2 cut(s) 307, 423
BsmBI CGTCTC 1 cut(s) 307
BsnI GGCC 1 cut(s) 48
BsoBI CYCGRG 1 cut(s) 127
Bsp143I GATC 2 cut(s) 117, 349
Bsp19I CCATGG 1 cut(s) 466
BspACI CCGC 4 cut(s) 251, 272, 408, 459
BspANI GGCC 1 cut(s) 48
BspCNI CTCAG 1 cut(s) 105
BspQI GCTCTTC 1 cut(s) 189
BsrFI RCCGGY 2 cut(s) 229, 401
BsrI ACTGG 1 cut(s) 99
BssAI RCCGGY 2 cut(s) 229, 401
BssECI CCNNGG 4 cut(s) 43, 126, 127, 466
BssMI GATC 2 cut(s) 117, 349
BssNI GRCGYC 2 cut(s) 144, 463
BssT1I CCWWGG 1 cut(s) 466
Bst4CI ACNGT 1 cut(s) 436
Bst6I CTCTTC 3 cut(s) 189, 442, 528
BstACI GRCGYC 2 cut(s) 144, 463
BstBAI YACGTR 1 cut(s) 106
BstC8I GCNNGC 1 cut(s) 403
BstDEI CTNAG 2 cut(s) 92, 390
BstDSI CCRYGG 2 cut(s) 43, 466
BstF5I GGATG 3 cut(s) 163, 352, 385
BstH2I RGCGCY 1 cut(s) 302
BstHHI GCGC 1 cut(s) 301
BstKTI GATC 2 cut(s) 120, 352
BstMAI GTCTC 2 cut(s) 307, 423
BstMBI GATC 2 cut(s) 117, 349
BstMWI GCNNNNNNNGC 7 cut(s) 28, 129, 211, 278, 287, 296, 417
BstSCI CCNGG 2 cut(s) 126, 127
BstSNI TACGTA 1 cut(s) 106
BstV1I GCAGC 4 cut(s) 40, 89, 410, 417
BstV2I GAAGAC 1 cut(s) 525
BstXI CCANNNNNNTGG 1 cut(s) 473
BsuRI GGCC 1 cut(s) 48
BtgI CCRYGG 2 cut(s) 43, 466
BtsCI GGATG 3 cut(s) 163, 352, 385
BtsIMutI CAGTG 1 cut(s) 441
Cac8I GCNNGC 1 cut(s) 403
CfoI GCGC 1 cut(s) 301
Cfr10I RCCGGY 2 cut(s) 229, 401
Cfr9I CCCGGG 1 cut(s) 127
CseI GACGC 2 cut(s) 152, 471
Csp6I GTAC 2 cut(s) 107, 366
CspCI CAANNNNNGTGG 2 cut(s) 25, 60
CviAII CATG 2 cut(s) 154, 467
CviQI GTAC 2 cut(s) 107, 366
DdeI CTNAG 2 cut(s) 92, 390
DpnI GATC 2 cut(s) 119, 351
DpnII GATC 2 cut(s) 117, 349
DrdI GACNNNNNNGTC 1 cut(s) 158
DseDI GACNNNNNNGTC 1 cut(s) 158
EaeI YGGCCR 1 cut(s) 46
Eam1104I CTCTTC 3 cut(s) 189, 442, 528
EarI CTCTTC 3 cut(s) 189, 442, 528
Eco105I TACGTA 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 466
Eco57I CTGAAG 2 cut(s) 185, 239
Eco88I CYCGRG 1 cut(s) 127
EcoRI GAATTC 1 cut(s) 87
EcoT14I CCWWGG 1 cut(s) 466
ErhI CCWWGG 1 cut(s) 466
Esp3I CGTCTC 1 cut(s) 307
FaeI CATG 2 cut(s) 157, 470
FaiI YATR 7 cut(s) 139, 155, 311, 361, 468, 486, 494
FalI AAGNNNNNCTT 2 cut(s) 183, 215
FatI CATG 2 cut(s) 153, 466
FbaI TGATCA 1 cut(s) 349
Fnu4HI GCNGC 7 cut(s) 29, 78, 251, 273, 399, 406, 409
FokI GGATG 3 cut(s) 170, 359, 392
Fsp4HI GCNGC 7 cut(s) 29, 78, 251, 273, 399, 406, 409
GlaI GCGC 1 cut(s) 300
GluI GCNGC 7 cut(s) 29, 78, 251, 273, 399, 406, 409
GsaI CCCAGC 1 cut(s) 209
GsuI CTGGAG 1 cut(s) 95
HaeII RGCGCY 1 cut(s) 302
HaeIII GGCC 1 cut(s) 48
HapII CCGG 3 cut(s) 128, 230, 402
HgaI GACGC 2 cut(s) 152, 471
HhaI GCGC 1 cut(s) 301
Hin1I GRCGYC 2 cut(s) 144, 463
Hin1II CATG 2 cut(s) 157, 470
Hin6I GCGC 1 cut(s) 299
HinP1I GCGC 1 cut(s) 299
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HinfI GANTC 2 cut(s) 4, 62
HpaII CCGG 3 cut(s) 128, 230, 402
HphI GGTGA 2 cut(s) 244, 554
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 2 cut(s) 95, 164
Hpy188III TCNNGA 2 cut(s) 112, 428
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 1 cut(s) 370
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 2 cut(s) 28, 559
HpyF10VI GCNNNNNNNGC 7 cut(s) 28, 129, 211, 278, 287, 296, 417
HpyF3I CTNAG 2 cut(s) 92, 390
HpySE526I ACGT 1 cut(s) 105
Hsp92I GRCGYC 2 cut(s) 144, 463
Hsp92II CATG 2 cut(s) 157, 470
HspAI GCGC 1 cut(s) 299
KroI GCCGGC 1 cut(s) 401
KroNI GCCGGC 1 cut(s) 403
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 2 cut(s) 117, 349
LguI GCTCTTC 1 cut(s) 189
LmnI GCTCC 2 cut(s) 85, 128
LpnPI CCDG 7 cut(s) 112, 125, 141, 191, 242, 243, 415
Lsp1109I GCAGC 4 cut(s) 40, 89, 410, 417
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 3 cut(s) 101, 140, 172
MalI GATC 2 cut(s) 119, 351
MboI GATC 2 cut(s) 117, 349
MboII GAAGA 6 cut(s) 127, 203, 206, 459, 530, 545
MlsI TGGCCA 1 cut(s) 48
MluCI AATT 2 cut(s) 87, 354
MluNI TGGCCA 1 cut(s) 48
MmeI TCCRAC 1 cut(s) 142
MnlI CCTC 8 cut(s) 50, 158, 335, 432, 443, 499, 517, 529
Mox20I TGGCCA 1 cut(s) 48
MroNI GCCGGC 1 cut(s) 401
MroXI GAANNNNTTC 2 cut(s) 61, 198
MscI TGGCCA 1 cut(s) 48
MseI TTAA 2 cut(s) 183, 372
MslI CAYNNNNRTG 1 cut(s) 471
Msp20I TGGCCA 1 cut(s) 48
MspA1I CMGCKG 3 cut(s) 179, 205, 408
MspI CCGG 3 cut(s) 128, 230, 402
MspR9I CCNGG 2 cut(s) 128, 129
MwoI GCNNNNNNNGC 7 cut(s) 28, 129, 211, 278, 287, 296, 417
NaeI GCCGGC 1 cut(s) 403
NciI CCSGG 2 cut(s) 128, 129
NcoI CCATGG 1 cut(s) 466
NdeII GATC 2 cut(s) 117, 349
NgoMIV GCCGGC 1 cut(s) 401
NlaIII CATG 2 cut(s) 157, 470
NmuCI GTSAC 1 cut(s) 140
PciSI GCTCTTC 1 cut(s) 189
PdiI GCCGGC 1 cut(s) 403
PdmI GAANNNNTTC 2 cut(s) 61, 198
PfeI GAWTC 1 cut(s) 62
PkrI GCNGC 7 cut(s) 30, 79, 252, 274, 400, 407, 410
Ppu21I YACGTR 1 cut(s) 106
PspFI CCCAGC 1 cut(s) 205
PvuII CAGCTG 2 cut(s) 179, 205
RsaI GTAC 2 cut(s) 108, 367
RsaNI GTAC 2 cut(s) 107, 366
RseI CAYNNNNRTG 1 cut(s) 471
SapI GCTCTTC 1 cut(s) 189
SaqAI TTAA 2 cut(s) 183, 372
SatI GCNGC 7 cut(s) 29, 78, 251, 273, 399, 406, 409
Sau3AI GATC 2 cut(s) 117, 349
ScaI AGTACT 1 cut(s) 367
ScrFI CCNGG 2 cut(s) 128, 129
SmaI CCCGGG 1 cut(s) 129
SmiMI CAYNNNNRTG 1 cut(s) 471
SnaBI TACGTA 1 cut(s) 106
Sse9I AATT 2 cut(s) 87, 354
SsiI CCGC 4 cut(s) 251, 272, 408, 459
StyD4I CCNGG 2 cut(s) 126, 127
StyI CCWWGG 1 cut(s) 466
TaaI ACNGT 1 cut(s) 436
TaiI ACGT 1 cut(s) 108
TaqI TCGA 1 cut(s) 427
TasI AATT 2 cut(s) 87, 354
TatI WGTACW 1 cut(s) 365
TauI GCSGC 3 cut(s) 253, 275, 411
TfiI GAWTC 1 cut(s) 62
Tru1I TTAA 2 cut(s) 183, 372
Tru9I TTAA 2 cut(s) 183, 372
TscAI CASTG 1 cut(s) 441
TseFI GTSAC 1 cut(s) 140
TseI GCWGC 4 cut(s) 28, 77, 398, 405
Tsp45I GTSAC 1 cut(s) 140
TspDTI ATGAA 1 cut(s) 413
TspGWI ACGGA 2 cut(s) 32, 331
TspMI CCCGGG 1 cut(s) 127
TspRI CASTG 1 cut(s) 441
XapI RAATTY 1 cut(s) 87
XcmI CCANNNNNNNNNTGG 1 cut(s) 473
XmaI CCCGGG 1 cut(s) 127
XmnI GAANNNNTTC 2 cut(s) 61, 198
ZrmI AGTACT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.