MD10G1084300.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014652
Physical Location & Seq
Reverse (-)
12381469 .. 12382023
555 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1084300.v1.1.491

Sequence Viewer

Length: 555 bp
ATGGGGATTTGCAACTCATGCGAATCAATCTCTGTAGCTACAGCCAAGTTGATTCTTCAAAACGGGCAGCTTCAAGAATTCTCGAAACCGATTAAGGTCTCACATGCATTGCAGATGATGAACGTCAGTCCCATGCATTTCATATGCGACTCAGATTACATGGAGTTTGGAGGCTTTTTTAGTGCTGTTGATGGGGATGAAGTGCTTCAGTTGGGTCAGCTTTACTTTGTGTTACCGTTGGGTTGGCTGAAAAAACCACTTCGACCGGAGCAGATGGCTGCTTTGGCCGTTAGGGCAAGTTTGGCGCTTACAAATATGAGACGGAGATATGGGGGCGGGTGTAAAAGTGCCAGTCTAGTTGTGTTTACTATTAGTCGGATGGCGGACAGGGTAGGTGGTGACAGCGGCATAAGTGATGGAGTCGGACATCGTGAAGTTGTGAGGAGAAGAAGAAGAGGAGGGCGCCGCTATAACCGTCGTGGTGGAAGTGGTAGCTATAGGACTAGGTTGAGTGTAGTTTTAGAGGAGGATGAGAAGATAGAGGAGGGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

185

Amino Acids

20.33

Weight (kDa)

9.5

Isoelectric Point (pI)

64.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 173 1.2e-22 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014652)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g07750
malus_domestica MD10G1084300.v1.1
prunus_persica Prupe.8G107500_v2.0.a1
pyrus_communis pycom10g06630
rosa_chinensis RchiOBHm_Chr6g0262101 RchiOBHm_Chr6g0271951
rosa_laevigata RLG00000014453
rosa_multiflora Rmu_sc0002510.1_g000007 Rmu_ssc0000125.1_g000021
rosa_roxburghii Rroxscaffold_7G00207780 Rroxscaffold_7G00207820
rosa_rugosa Rorug05G0595200
rosa_samantha Rh6AG115000 Rh6BG109000 Rh6CG106300 Rh6DG096700
rosa_wichuraiana Rw6G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 462
AciI CCGC 4 cut(s) 336, 383, 405, 466
AcoI YGGCCR 1 cut(s) 285
AcsI RAATTY 1 cut(s) 77
AcuI CTGAAG 1 cut(s) 191
AcyI GRCGYC 1 cut(s) 463
AgsI TTSAA 2 cut(s) 59, 74
AluBI AGCT 4 cut(s) 38, 70, 220, 495
AluI AGCT 4 cut(s) 38, 70, 220, 495
Alw26I GTCTC 2 cut(s) 103, 313
AoxI GGCC 1 cut(s) 285
ApeKI GCWGC 2 cut(s) 67, 278
ApoI RAATTY 1 cut(s) 77
Asp700I GAANNNNTTC 1 cut(s) 204
AspLEI GCGC 2 cut(s) 307, 465
AsuHPI GGTGA 1 cut(s) 410
BanI GGYRCC 1 cut(s) 462
BbvI GCAGC 2 cut(s) 79, 265
BccI CCATC 4 cut(s) 185, 268, 373, 410
BceAI ACGGC 1 cut(s) 272
BcoDI GTCTC 2 cut(s) 103, 313
BfaI CTAG 2 cut(s) 356, 504
BfmI CTRYAG 3 cut(s) 33, 39, 496
BfoI RGCGCY 2 cut(s) 308, 466
BglI GCCNNNNNGGC 1 cut(s) 293
BisI GCNGC 4 cut(s) 68, 279, 406, 466
BlsI GCNGC 4 cut(s) 69, 280, 407, 467
BmiI GGNNCC 1 cut(s) 464
BsaHI GRCGYC 1 cut(s) 463
BsaI GGTCTC 1 cut(s) 103
BsaWI WCCGGW 1 cut(s) 265
Bse1I ACTGG 1 cut(s) 351
Bse3DI GCAATG 1 cut(s) 107
BseGI GGATG 3 cut(s) 202, 384, 535
BseMI GCAATG 1 cut(s) 107
BseMII CTCAG 1 cut(s) 165
BseNI ACTGG 1 cut(s) 351
BseRI GAGGAG 3 cut(s) 457, 471, 539
BseXI GCAGC 2 cut(s) 79, 265
Bsh1285I CGRYCG 1 cut(s) 266
BshFI GGCC 1 cut(s) 287
BshNI GGYRCC 1 cut(s) 462
BsiEI CGRYCG 1 cut(s) 266
BsiSI CCGG 1 cut(s) 266
BslFI GGGAC 1 cut(s) 114
BsmAI GTCTC 2 cut(s) 103, 313
BsmBI CGTCTC 1 cut(s) 313
BsmFI GGGAC 1 cut(s) 114
BsnI GGCC 1 cut(s) 287
Bso31I GGTCTC 1 cut(s) 103
BspACI CCGC 4 cut(s) 336, 383, 405, 466
BspANI GGCC 1 cut(s) 287
BspCNI CTCAG 1 cut(s) 164
BspLI GGNNCC 1 cut(s) 464
BspT107I GGYRCC 1 cut(s) 462
BspTNI GGTCTC 1 cut(s) 103
BsrDI GCAATG 1 cut(s) 107
BsrI ACTGG 1 cut(s) 351
BssNI GRCGYC 1 cut(s) 463
Bst4CI ACNGT 2 cut(s) 237, 476
Bst6I CTCTTC 1 cut(s) 448
BstACI GRCGYC 1 cut(s) 463
BstAPI GCANNNNNTGC 1 cut(s) 18
BstDEI CTNAG 1 cut(s) 151
BstF5I GGATG 3 cut(s) 202, 384, 535
BstH2I RGCGCY 2 cut(s) 308, 466
BstHHI GCGC 2 cut(s) 307, 465
BstMAI GTCTC 2 cut(s) 103, 313
BstMCI CGRYCG 1 cut(s) 266
BstMWI GCNNNNNNNGC 4 cut(s) 18, 284, 293, 302
BstNSI RCATGY 1 cut(s) 107
BstSFI CTRYAG 3 cut(s) 33, 39, 496
BstV1I GCAGC 2 cut(s) 79, 265
BsuRI GGCC 1 cut(s) 287
BtsCI GGATG 3 cut(s) 202, 384, 535
CfoI GCGC 2 cut(s) 307, 465
CviAII CATG 4 cut(s) 18, 104, 133, 160
CviJI RGCY 9 cut(s) 38, 44, 70, 174, 220, 247, 278, 287, 495
CviKI_1 RGCY 9 cut(s) 38, 44, 70, 174, 220, 247, 278, 287, 495
DdeI CTNAG 1 cut(s) 151
DinI GGCGCC 1 cut(s) 464
EaeI YGGCCR 1 cut(s) 285
Eam1104I CTCTTC 1 cut(s) 448
EarI CTCTTC 1 cut(s) 448
EciI GGCGGA 1 cut(s) 398
Eco31I GGTCTC 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 191
EcoRI GAATTC 1 cut(s) 77
EcoT22I ATGCAT 2 cut(s) 109, 138
EgeI GGCGCC 1 cut(s) 464
EheI GGCGCC 1 cut(s) 464
Esp3I CGTCTC 1 cut(s) 313
FaeI CATG 4 cut(s) 21, 107, 136, 163
FaqI GGGAC 1 cut(s) 114
FatI CATG 4 cut(s) 17, 103, 132, 159
FauI CCCGC 1 cut(s) 329
FauNDI CATATG 1 cut(s) 143
Fnu4HI GCNGC 4 cut(s) 68, 279, 406, 466
FokI GGATG 3 cut(s) 209, 391, 542
Fsp4HI GCNGC 4 cut(s) 68, 279, 406, 466
FspBI CTAG 2 cut(s) 356, 504
GlaI GCGC 2 cut(s) 306, 464
GluI GCNGC 4 cut(s) 68, 279, 406, 466
HaeII RGCGCY 2 cut(s) 308, 466
HaeIII GGCC 1 cut(s) 287
HapII CCGG 1 cut(s) 266
HhaI GCGC 2 cut(s) 307, 465
Hin1I GRCGYC 1 cut(s) 463
Hin1II CATG 4 cut(s) 21, 107, 136, 163
Hin6I GCGC 2 cut(s) 305, 463
HinP1I GCGC 2 cut(s) 305, 463
HinfI GANTC 4 cut(s) 23, 52, 149, 420
HpaII CCGG 1 cut(s) 266
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 1 cut(s) 366
Hpy188I TCNGA 3 cut(s) 154, 378, 425
Hpy188III TCNNGA 3 cut(s) 74, 82, 431
Hpy8I GTNNAC 1 cut(s) 366
Hpy99I CGWCG 1 cut(s) 480
HpyCH4III ACNGT 2 cut(s) 237, 476
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 5 cut(s) 12, 107, 112, 136, 551
HpyF10VI GCNNNNNNNGC 4 cut(s) 18, 284, 293, 302
HpyF3I CTNAG 1 cut(s) 151
HpySE526I ACGT 1 cut(s) 123
Hsp92I GRCGYC 1 cut(s) 463
Hsp92II CATG 4 cut(s) 21, 107, 136, 163
HspAI GCGC 2 cut(s) 305, 463
KasI GGCGCC 1 cut(s) 462
LmnI GCTCC 1 cut(s) 268
LpnPI CCDG 3 cut(s) 279, 364, 373
Lsp1109I GCAGC 2 cut(s) 79, 265
MaeI CTAG 2 cut(s) 356, 504
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 2 cut(s) 231, 398
MboII GAAGA 5 cut(s) 47, 459, 462, 465, 547
MluCI AATT 1 cut(s) 77
Mly113I GGCGCC 1 cut(s) 463
MlyI GAGTC 2 cut(s) 143, 429
MmeI TCCRAC 2 cut(s) 356, 403
MnlI CCTC 8 cut(s) 164, 435, 449, 452, 517, 520, 535, 538
Mph1103I ATGCAT 2 cut(s) 109, 138
MroXI GAANNNNTTC 1 cut(s) 204
MseI TTAA 1 cut(s) 93
MspA1I CMGCKG 1 cut(s) 405
MspI CCGG 1 cut(s) 266
MwoI GCNNNNNNNGC 4 cut(s) 18, 284, 293, 302
NarI GGCGCC 1 cut(s) 463
NdeI CATATG 1 cut(s) 143
NlaIII CATG 4 cut(s) 21, 107, 136, 163
NlaIV GGNNCC 1 cut(s) 464
NmuCI GTSAC 1 cut(s) 398
NsiI ATGCAT 2 cut(s) 109, 138
NspI RCATGY 1 cut(s) 107
PdmI GAANNNNTTC 1 cut(s) 204
PfeI GAWTC 2 cut(s) 23, 52
PkrI GCNGC 4 cut(s) 69, 280, 407, 467
PleI GAGTC 2 cut(s) 143, 428
PluTI GGCGCC 1 cut(s) 466
PpsI GAGTC 2 cut(s) 143, 428
PspN4I GGNNCC 1 cut(s) 464
SaqAI TTAA 1 cut(s) 93
SatI GCNGC 4 cut(s) 68, 279, 406, 466
SchI GAGTC 2 cut(s) 143, 429
SetI ASST 8 cut(s) 40, 72, 99, 126, 222, 397, 497, 509
SfcI CTRYAG 3 cut(s) 33, 39, 496
SfoI GGCGCC 1 cut(s) 464
Sse9I AATT 1 cut(s) 77
SsiI CCGC 4 cut(s) 336, 383, 405, 466
SspDI GGCGCC 1 cut(s) 462
SspMI CTAG 2 cut(s) 356, 504
TaaI ACNGT 2 cut(s) 237, 476
TaiI ACGT 1 cut(s) 126
TaqI TCGA 2 cut(s) 83, 262
TasI AATT 1 cut(s) 77
TauI GCSGC 2 cut(s) 408, 468
TfiI GAWTC 2 cut(s) 23, 52
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TseFI GTSAC 1 cut(s) 398
TseI GCWGC 2 cut(s) 67, 278
Tsp45I GTSAC 1 cut(s) 398
TspDTI ATGAA 3 cut(s) 130, 134, 213
TspGWI ACGGA 1 cut(s) 337
XapI RAATTY 1 cut(s) 77
XceI RCATGY 1 cut(s) 107
XmnI GAANNNNTTC 1 cut(s) 204
XspI CTAG 2 cut(s) 356, 504
Zsp2I ATGCAT 2 cut(s) 109, 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.