Rh6AG115000

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0014652
Physical Location & Seq
Forward (+)
17343682 .. 17344672
991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG115000.1

Sequence Viewer

Length: 558 bp
ATGGGCATTTGCAGCTCGTCAACCTCCGTGGCCACAGCCAAATTGATTCTCCAAGATGGGCAGCTCCAAGAATTCTCAGACCCAGTTAGGGCATCCTACGTACTGCAGAAGATCAGCTCCCCGGGCTTTGTATGTGACGCCGACGACATGGATTTCGGAGGCTTTGTTACAGCTGTTAATGGGGAAGAAGAGCTTCAGCTGGGTCAGCTTTACTTTGTGTTGCCGGTGAGTTGGTTGAATAAGCCGCTTCGGGCAGAGGAGATGGCGGGTTTGGCTGTTAGGGCAAGTTTGGCGCTTGGCAATGTGAGACGGAGTAATAGGTGTTGTTGTAAAGGAGGGGATGATCAAATTGTATTTAGTACTATTAAGAAGGATGTCAAGCCTAAGATGGCAGCCGGCAGCGGCAGTTTTGGCGTTCATCGAGACACAGTGAGGAAAAGAAGAGGGAGAAGCGGACGCCATGGTGGTGGTCGAAGTTATACCTCTATGGCAAAGTTGAGTGTGATTTTGGAAGACGAGGATAGGGAAGAGGGTGGTGAGATTTGGGTTCTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

185

Amino Acids

20.08

Weight (kDa)

7.61

Isoelectric Point (pI)

58.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 118 3.2e-23 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014652)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g07750
malus_domestica MD10G1084300.v1.1
prunus_persica Prupe.8G107500_v2.0.a1
pyrus_communis pycom10g06630
rosa_chinensis RchiOBHm_Chr6g0262101 RchiOBHm_Chr6g0271951
rosa_laevigata RLG00000014453
rosa_multiflora Rmu_sc0002510.1_g000007 Rmu_ssc0000125.1_g000021
rosa_roxburghii Rroxscaffold_7G00207780 Rroxscaffold_7G00207820
rosa_rugosa Rorug05G0595200
rosa_samantha Rh6AG115000 Rh6BG109000 Rh6CG106300 Rh6DG096700
rosa_wichuraiana Rw6G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 245, 266, 402, 453
AcoI YGGCCR 1 cut(s) 30
AcsI RAATTY 1 cut(s) 71
AcuI CTGAAG 1 cut(s) 179
AcyI GRCGYC 2 cut(s) 138, 457
AfaI GTAC 2 cut(s) 102, 361
AfiI CCNNNNNNNGG 1 cut(s) 88
AgsI TTSAA 1 cut(s) 238
AluBI AGCT 7 cut(s) 15, 64, 117, 173, 193, 199, 208
AluI AGCT 7 cut(s) 15, 64, 117, 173, 193, 199, 208
Alw26I GTCTC 2 cut(s) 301, 417
Ama87I CYCGRG 1 cut(s) 121
AoxI GGCC 1 cut(s) 30
ApeKI GCWGC 4 cut(s) 12, 61, 392, 399
ApoI RAATTY 1 cut(s) 71
ArsI GACNNNNNNTTYG 2 cut(s) 137, 169
Asp700I GAANNNNTTC 1 cut(s) 192
AspLEI GCGC 1 cut(s) 295
AsuC2I CCSGG 2 cut(s) 122, 123
AsuHPI GGTGA 2 cut(s) 238, 548
AvaI CYCGRG 1 cut(s) 121
BalI TGGCCA 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 519
BbvI GCAGC 4 cut(s) 24, 73, 404, 411
BccI CCATC 3 cut(s) 50, 256, 382
BclI TGATCA 1 cut(s) 343
BcnI CCSGG 2 cut(s) 122, 123
BcoDI GTCTC 2 cut(s) 301, 417
BfmI CTRYAG 1 cut(s) 104
BfoI RGCGCY 1 cut(s) 296
BisI GCNGC 6 cut(s) 13, 62, 245, 393, 400, 403
BlsI GCNGC 6 cut(s) 14, 63, 246, 394, 401, 404
BmcAI AGTACT 1 cut(s) 361
Bme1390I CCNGG 2 cut(s) 122, 123
BmeT110I CYCGRG 1 cut(s) 121
BmrFI CCNGG 2 cut(s) 122, 123
BmrI ACTGGG 1 cut(s) 77
BmsI GCATC 1 cut(s) 101
BmuI ACTGGG 1 cut(s) 77
BpiI GAAGAC 1 cut(s) 519
BpuMI CCSGG 2 cut(s) 122, 123
BsaAI YACGTR 1 cut(s) 100
BsaHI GRCGYC 2 cut(s) 138, 457
BsaJI CCNNGG 4 cut(s) 27, 120, 121, 460
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse118I RCCGGY 2 cut(s) 223, 395
Bse1I ACTGG 1 cut(s) 83
Bse3DI GCAATG 1 cut(s) 307
BseDI CCNNGG 4 cut(s) 27, 120, 121, 460
BseGI GGATG 3 cut(s) 92, 346, 379
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMI GCAATG 1 cut(s) 307
BseMII CTCAG 1 cut(s) 90
BseNI ACTGG 1 cut(s) 83
BseRI GAGGAG 1 cut(s) 272
BseXI GCAGC 4 cut(s) 24, 73, 404, 411
BseYI CCCAGC 1 cut(s) 199
BshFI GGCC 1 cut(s) 32
BsiHKCI CYCGRG 1 cut(s) 121
BsiSI CCGG 3 cut(s) 122, 224, 396
BslI CCNNNNNNNGG 1 cut(s) 88
BsmAI GTCTC 2 cut(s) 301, 417
BsmBI CGTCTC 1 cut(s) 301
BsnI GGCC 1 cut(s) 32
BsoBI CYCGRG 1 cut(s) 121
Bsp143I GATC 2 cut(s) 111, 343
Bsp19I CCATGG 1 cut(s) 460
BspACI CCGC 4 cut(s) 245, 266, 402, 453
BspANI GGCC 1 cut(s) 32
BspCNI CTCAG 1 cut(s) 89
BspMAI CTGCAG 1 cut(s) 108
BspQI GCTCTTC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 307
BsrFI RCCGGY 2 cut(s) 223, 395
BsrI ACTGG 1 cut(s) 83
BssAI RCCGGY 2 cut(s) 223, 395
BssECI CCNNGG 4 cut(s) 27, 120, 121, 460
BssMI GATC 2 cut(s) 111, 343
BssNI GRCGYC 2 cut(s) 138, 457
BssT1I CCWWGG 1 cut(s) 460
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 3 cut(s) 183, 436, 522
BstACI GRCGYC 2 cut(s) 138, 457
BstBAI YACGTR 1 cut(s) 100
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 2 cut(s) 76, 384
BstDSI CCRYGG 2 cut(s) 27, 460
BstF5I GGATG 3 cut(s) 92, 346, 379
BstH2I RGCGCY 1 cut(s) 296
BstHHI GCGC 1 cut(s) 295
BstKTI GATC 2 cut(s) 114, 346
BstMAI GTCTC 2 cut(s) 301, 417
BstMBI GATC 2 cut(s) 111, 343
BstMWI GCNNNNNNNGC 7 cut(s) 12, 123, 205, 272, 281, 290, 411
BstSCI CCNGG 2 cut(s) 120, 121
BstSFI CTRYAG 1 cut(s) 104
BstSNI TACGTA 1 cut(s) 100
BstV1I GCAGC 4 cut(s) 24, 73, 404, 411
BstV2I GAAGAC 1 cut(s) 519
BstXI CCANNNNNNTGG 1 cut(s) 467
BsuRI GGCC 1 cut(s) 32
BtgI CCRYGG 2 cut(s) 27, 460
BtsCI GGATG 3 cut(s) 92, 346, 379
BtsIMutI CAGTG 1 cut(s) 435
Cac8I GCNNGC 1 cut(s) 397
CfoI GCGC 1 cut(s) 295
Cfr10I RCCGGY 2 cut(s) 223, 395
Cfr9I CCCGGG 1 cut(s) 121
CseI GACGC 2 cut(s) 146, 465
Csp6I GTAC 2 cut(s) 101, 360
CspCI CAANNNNNGTGG 2 cut(s) 9, 44
CviAII CATG 2 cut(s) 148, 461
CviQI GTAC 2 cut(s) 101, 360
DdeI CTNAG 2 cut(s) 76, 384
DpnI GATC 2 cut(s) 113, 345
DpnII GATC 2 cut(s) 111, 343
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 3 cut(s) 183, 436, 522
EarI CTCTTC 3 cut(s) 183, 436, 522
Eco105I TACGTA 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 460
Eco57I CTGAAG 1 cut(s) 179
Eco88I CYCGRG 1 cut(s) 121
EcoRI GAATTC 1 cut(s) 71
EcoT14I CCWWGG 1 cut(s) 460
ErhI CCWWGG 1 cut(s) 460
Esp3I CGTCTC 1 cut(s) 301
FaeI CATG 2 cut(s) 151, 464
FaiI YATR 5 cut(s) 133, 149, 462, 480, 488
FalI AAGNNNNNCTT 2 cut(s) 177, 209
FatI CATG 2 cut(s) 147, 460
FauI CCCGC 1 cut(s) 259
FbaI TGATCA 1 cut(s) 343
Fnu4HI GCNGC 6 cut(s) 13, 62, 245, 393, 400, 403
FokI GGATG 3 cut(s) 79, 353, 386
Fsp4HI GCNGC 6 cut(s) 13, 62, 245, 393, 400, 403
GlaI GCGC 1 cut(s) 294
GluI GCNGC 6 cut(s) 13, 62, 245, 393, 400, 403
GsaI CCCAGC 1 cut(s) 203
HaeII RGCGCY 1 cut(s) 296
HaeIII GGCC 1 cut(s) 32
HapII CCGG 3 cut(s) 122, 224, 396
HgaI GACGC 2 cut(s) 146, 465
HhaI GCGC 1 cut(s) 295
Hin1I GRCGYC 2 cut(s) 138, 457
Hin1II CATG 2 cut(s) 151, 464
Hin6I GCGC 1 cut(s) 293
HinP1I GCGC 1 cut(s) 293
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HinfI GANTC 1 cut(s) 46
HpaII CCGG 3 cut(s) 122, 224, 396
HphI GGTGA 2 cut(s) 238, 548
Hpy166II GTNNAC 1 cut(s) 21
Hpy188I TCNGA 2 cut(s) 79, 158
Hpy188III TCNNGA 1 cut(s) 422
Hpy8I GTNNAC 1 cut(s) 21
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 1 cut(s) 364
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 3 cut(s) 12, 106, 553
HpyF10VI GCNNNNNNNGC 7 cut(s) 12, 123, 205, 272, 281, 290, 411
HpyF3I CTNAG 2 cut(s) 76, 384
HpySE526I ACGT 1 cut(s) 99
Hsp92I GRCGYC 2 cut(s) 138, 457
Hsp92II CATG 2 cut(s) 151, 464
HspAI GCGC 1 cut(s) 293
KroI GCCGGC 1 cut(s) 395
KroNI GCCGGC 1 cut(s) 397
Ksp22I TGATCA 1 cut(s) 343
Kzo9I GATC 2 cut(s) 111, 343
LguI GCTCTTC 1 cut(s) 183
LmnI GCTCC 2 cut(s) 69, 122
LpnPI CCDG 5 cut(s) 96, 135, 185, 237, 409
Lsp1109I GCAGC 4 cut(s) 24, 73, 404, 411
LweI GCATC 1 cut(s) 101
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 2 cut(s) 134, 166
MalI GATC 2 cut(s) 113, 345
MboI GATC 2 cut(s) 111, 343
MboII GAAGA 6 cut(s) 121, 197, 200, 453, 524, 539
MlsI TGGCCA 1 cut(s) 32
MluCI AATT 3 cut(s) 41, 71, 348
MluNI TGGCCA 1 cut(s) 32
MnlI CCTC 9 cut(s) 34, 152, 250, 329, 426, 437, 493, 511, 523
Mox20I TGGCCA 1 cut(s) 32
MroNI GCCGGC 1 cut(s) 395
MroXI GAANNNNTTC 1 cut(s) 192
MscI TGGCCA 1 cut(s) 32
MseI TTAA 2 cut(s) 177, 366
MslI CAYNNNNRTG 1 cut(s) 465
Msp20I TGGCCA 1 cut(s) 32
MspA1I CMGCKG 3 cut(s) 173, 199, 402
MspI CCGG 3 cut(s) 122, 224, 396
MspR9I CCNGG 2 cut(s) 122, 123
MwoI GCNNNNNNNGC 7 cut(s) 12, 123, 205, 272, 281, 290, 411
NaeI GCCGGC 1 cut(s) 397
NciI CCSGG 2 cut(s) 122, 123
NcoI CCATGG 1 cut(s) 460
NdeII GATC 2 cut(s) 111, 343
NgoMIV GCCGGC 1 cut(s) 395
NlaIII CATG 2 cut(s) 151, 464
NmuCI GTSAC 1 cut(s) 134
PciSI GCTCTTC 1 cut(s) 183
PdiI GCCGGC 1 cut(s) 397
PdmI GAANNNNTTC 1 cut(s) 192
PfeI GAWTC 1 cut(s) 46
PkrI GCNGC 6 cut(s) 14, 63, 246, 394, 401, 404
Ppu21I YACGTR 1 cut(s) 100
PspFI CCCAGC 1 cut(s) 199
PstI CTGCAG 1 cut(s) 108
PvuII CAGCTG 2 cut(s) 173, 199
RsaI GTAC 2 cut(s) 102, 361
RsaNI GTAC 2 cut(s) 101, 360
RseI CAYNNNNRTG 1 cut(s) 465
SapI GCTCTTC 1 cut(s) 183
SaqAI TTAA 2 cut(s) 177, 366
SatI GCNGC 6 cut(s) 13, 62, 245, 393, 400, 403
Sau3AI GATC 2 cut(s) 111, 343
ScaI AGTACT 1 cut(s) 361
ScrFI CCNGG 2 cut(s) 122, 123
SfaNI GCATC 1 cut(s) 101
SfcI CTRYAG 1 cut(s) 104
SmaI CCCGGG 1 cut(s) 123
SmiMI CAYNNNNRTG 1 cut(s) 465
SnaBI TACGTA 1 cut(s) 100
Sse9I AATT 3 cut(s) 41, 71, 348
SsiI CCGC 4 cut(s) 245, 266, 402, 453
StyD4I CCNGG 2 cut(s) 120, 121
StyI CCWWGG 1 cut(s) 460
TaaI ACNGT 1 cut(s) 430
TaiI ACGT 1 cut(s) 102
TaqI TCGA 2 cut(s) 421, 472
TasI AATT 3 cut(s) 41, 71, 348
TatI WGTACW 1 cut(s) 359
TauI GCSGC 2 cut(s) 247, 405
TfiI GAWTC 1 cut(s) 46
Tru1I TTAA 2 cut(s) 177, 366
Tru9I TTAA 2 cut(s) 177, 366
TscAI CASTG 1 cut(s) 435
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 4 cut(s) 12, 61, 392, 399
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 1 cut(s) 407
TspGWI ACGGA 2 cut(s) 16, 325
TspMI CCCGGG 1 cut(s) 121
TspRI CASTG 1 cut(s) 435
XapI RAATTY 1 cut(s) 71
XmaI CCCGGG 1 cut(s) 121
XmnI GAANNNNTTC 1 cut(s) 192
ZrmI AGTACT 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.