Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0013282

Physical Location & Seq

Genomic Coordinates Reverse (-)

8079713 .. 8086075

Gene Structure 6363 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g09160.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2541 bp

ATGGGCAAGGTGAAAGGAAAGCATCGTCTTGATAAGTACTACCACCTCGCCAAAGAGCACGGCTACCGCTCCCGTGCCTCATGGAAGCTCCTCCAGCTCGACGCCAAGCACTCCTTCCTCCACTCCTCCCACGCAGTCCTCGATCTCTGCGCCGCCCCCGGCGGTTGGATGCAAATCGCCGTCCAGCGCGTCCCCGTCGGCAGCTTCGTCGTCGGCGTCGACTTGGTCCCCATCGCTCCTGTTCGCGGCGCCTTCTCTATCCAGCAGGACATAACGAGGACCGAGTGCGTCGCCAAGCTGAGGAGGCTGATGAAGGAGAACGGCTGCTCCGCCTTTGATTTGGTGCTTCATGATGGCTCCCCCAACGTCGGTGGAGCCTGGTCGTCGGAGGCTACGTCGCAGAACGCTTTGGTCATTGACTCGGTGAAGCTGGCCACGCAGCTCCTGGCTCCCAAGGGCACATTTGTCACCAAGATCTTCAGGTCACAAGATTACAATGCTGTCTTGTTTTGCCTCAAGGAGTTATTTGAGAAGGTTGAACAGTATAAACCATCAGCTAGTCGTAGTGCATCTGCGGAGACATATCTTTTGGGATTTAAGTACAAGGCTCCTGCAAAGATTGATCCTCGTCTGCTTGATATGAAGCATCTGTTTAAAGCCGTCGAACCTCCGAGGAAGGTGGTGGATGTACTAAAAGGATCAAAGCAAAAAAGACACCGTGATGGGTATGAAGATGGAGACACAATTTTGAGGAAAGTATCTGCAGCCGCTGACTTCATTTGGTCTGAGAGTCCTCTTGATATTCTTGGGTCTGTTACTTCCATAACATTTGATGATGTGACATCTTTGCCTATCAAGGATCATGGTTTGACAACAGAGGAGGTCAAAATCCTATGTGATGATCTGCGTGTTCTGGGGAAGCAAGATTTCAAGCATCTTTTGAAGTGGCGGGTGCAGATAAGAAAGGCCTTGTCTCCTTCTGAGAAAGCTACTGTTACTACTGCCCCAGATGTTGAAAAGGAGAATAAGGAGGATGATGATGACAGAATACTGAATGAAATGGAAGAGCTTACAAATGCTATGGAGCGCAAGAAGAAACGAGAAAAGAAGCTACAAGCAAAGAAGCGTGCTCAGAACAAGGTCCGGAAGGCAACAGGGATGCAAATAGATGCAATGCAAGATGGTTATACAGATGATACTTTATTTTCCTTATCTGCAATCAAGGGTAAGAAAGATCTGGTAGCGATTGATTCCACTGAATATGATGATGAGAATGGGGAGTTGGCAGATAGCGAAAACGAGGATAGGCAAGAGAAACCTGAGGAAGCATCTTCCAGTGATGTAGACTCTGATGACGGACGTAGAAGATATGATGCAAAAATGGAAGAGCTTCTAGATCAAGCTTATGAACATTATGTGACCAAAAAGGAGGGAAGTGCAAAGCAGCGAAAGCGTTTGAAAGAAGAGGGTCAAAGTTTGGAGGATGTTGACGGTGAAGATACATTTCCATCTGATTACGATTCAGACAAAGATGAGGCTGATCAGGAAAAGAATCCTCTCTTAGATGCTCTTGATGATGGGGAAGGACCAACTCAGGAGGAGGTTACAAATAATTGGTTTAGTCAGTCAATCTTTGCAGAAGCTGTAGAGCAGGGGGATTTGGAGAAGTCTGATAGTGAAGACGAAATGGAGGTTGGAAGTCCAGAAGAGAAATTGCCCCTGCAAGAGAAGCCAAAGGAAAAGACTGTTGTTAAAAATGTTAGAGAAAAGCCTGAAAATCGTGTTATAGGGTCCGATTCCGCTCATGAAGTCAAAGCTAAGAAGGCAGATGACGATTTTGAGATTGTCCCTGCTCCTGATACAGATTCAAGTGATGATTCATCATCAGATGAGTCGGAGGATATGGATACCTATAGAAAAGCTGAGATACTGGCATGTGCCAAAAAAATGTTGTCAAAGAAACAAAGGGAACAGATGCTTGATGATGCGTATAATAAGTACATGCTTGATGATGAAGGCTTGCCCAAATGGTTTTTGGATGAGGAAAAGAAGCACCGACGACCAAATAAGCCAGTGACCAAAGAAGAGATCAATGCAATGAAAGCGCAGTTTAAGGAAATTGATGCCCGACCGGCAAAGAAGGTGGCAGAGGCTAAAGCTCGAAAGAAGCGGGTTGCCATGAGGAAGCTTGAGAAGATTCGCAAGAAGGCCAACACAATATCAGATCAGGCAGATATCTCTGATCGTTCTAAGAGCAAGCAAATAGACCAATTGTACAAGAAGGCTTTGCCCAAGAGGCCTAAAAAGGAGTATGTGGTTGCGAAGAAAGGAGTTCAAGTCAAGGTTGGCCCGGGCAAAGTCCGTGTTGATGCACGAATGAAAAAGGATGCAAGGGGTCGTGGAACAGGTAACAAATGGAAAGGAAAAGGCAGTGAAAAGAAAGGGAAAAATGCGAAGGACAAAAGAGGTAAAGGATCTGGAGGGAAGCCTTCAGGAAAGAAGGGCAGTACCAAAGGGAGAAAGATGGGCATGCATGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000460 GO:0000463 GO:0000466 GO:0000470 GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008173 GO:0008649 GO:0008650 GO:0008757 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016435 GO:0016436 GO:0016740 GO:0016741 GO:0022613 GO:0030684 GO:0030687 GO:0031167 GO:0031974 GO:0031981 GO:0032259 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042273 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:0140102 GO:1901360 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3381 FtsJ Spb1_C

Protein Analysis

847

Amino Acids

95.19

Weight (kDa)

8.62

Isoelectric Point (pI)

41.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FtsJ	PF01728	22 - 201	2.4e-46	FtsJ-like methyltransferase
DUF3381	PF11861	234 - 382	1.4e-44	Ribosomal RNA methyltransferase Spb1, DUF3381
Spb1_C	PF07780	607 - 801	5.7e-60	Spb1 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013282)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25730
fragaria_vesca	FvH4_2g09160
malus_domestica	MD05G1064300.v1.1 MD10G1073000.v1.1
prunus_persica	Prupe.8G094400_v2.0.a1
pyrus_communis	pycom05g05540 pycom10g05760
rosa_chinensis	RchiOBHm_Chr6g0265021
rosa_laevigata	RLG00000014215 RLG00000014220
rosa_multiflora	Rmu_sc0002934.1_g000022
rosa_roxburghii	Rroxscaffold_175G00432100
rosa_rugosa	Rorug06G0013800
rosa_samantha	Rh6AG136200 Rh6BG133000 Rh6CG131600 Rh6DG118800
rosa_wichuraiana	Rw6G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	248
AccBSI	CCGCTC	2 cut(s)	69, 1802
AccI	GTMKAC	2 cut(s)	219, 1344
AccII	CGCG	2 cut(s)	189, 246
AccIII	TCCGGA	1 cut(s)	1143
AclWI	GGATC	4 cut(s)	617, 706, 867, 2482
AcoI	YGGCCR	1 cut(s)	432
AcuI	CTGAAG	2 cut(s)	463, 2475
AcyI	GRCGYC	3 cut(s)	102, 216, 249
AfaI	GTAC	6 cut(s)	38, 602, 690, 2000, 2276, 2509
AfiI	CCNNNNNNNGG	4 cut(s)	165, 245, 300, 368
AgsI	TTSAA	7 cut(s)	539, 931, 943, 1016, 1459, 1869, 2336
AjnI	CCWGG	2 cut(s)	377, 444
AjuI	GAANNNNNNNTTGG	6 cut(s)	98, 130, 1265, 1297, 1677, 1709
AloI	GAACNNNNNNTCC	2 cut(s)	311, 343
Alw21I	GWGCWC	2 cut(s)	60, 1132
Alw26I	GTCTC	3 cut(s)	572, 732, 978
AlwI	GGATC	4 cut(s)	617, 706, 867, 2482
AlwNI	CAGNNNCTG	3 cut(s)	445, 770, 1643
Ama87I	CYCGRG	1 cut(s)	2350
Aor13HI	TCCGGA	1 cut(s)	1143
AoxI	GGCC	5 cut(s)	432, 966, 2208, 2297, 2347
ApeKI	GCWGC	5 cut(s)	201, 324, 439, 764, 1444
ArsI	GACNNNNNNTTYG	2 cut(s)	571, 603
Asp700I	GAANNNNTTC	1 cut(s)	1389
AspLEI	GCGC	5 cut(s)	152, 189, 251, 1089, 2107
AspS9I	GGNCC	6 cut(s)	226, 279, 1141, 1586, 1791, 2348
AsuC2I	CCSGG	3 cut(s)	159, 2351, 2352
AsuHPI	GGTGA	4 cut(s)	22, 436, 460, 1505
AvaI	CYCGRG	1 cut(s)	2350
AvaII	GGWCC	5 cut(s)	226, 279, 1141, 1586, 1791
AxyI	CCTNAGG	1 cut(s)	1320
BaeGI	GKGCMC	1 cut(s)	461
BalI	TGGCCA	1 cut(s)	434
BanI	GGYRCC	1 cut(s)	248
BbsI	GAAGAC	1 cut(s)	1686
Bbv12I	GWGCWC	2 cut(s)	60, 1132
BbvCI	CCTCAGC	1 cut(s)	299
BbvI	GCAGC	5 cut(s)	213, 311, 451, 776, 1456
BccI	CCATC	9 cut(s)	239, 347, 559, 716, 728, 1175, 1516, 1571, 2518
BceAI	ACGGC	4 cut(s)	76, 164, 337, 644
BcgI	CGANNNNNNTGC	2 cut(s)	190, 224
BciT130I	CCWGG	2 cut(s)	379, 446
BciVI	GTATCC	1 cut(s)	1900
BclI	TGATCA	1 cut(s)	1540
BcnI	CCSGG	3 cut(s)	159, 2351, 2352
BcoDI	GTCTC	3 cut(s)	572, 732, 978
BfaI	CTAG	2 cut(s)	558, 1394
BfmI	CTRYAG	3 cut(s)	762, 1644, 1912
BfoI	RGCGCY	1 cut(s)	252
BfuI	GTATCC	1 cut(s)	1900
BglI	GCCNNNNNGGC	2 cut(s)	2132, 2296
BglII	AGATCT	2 cut(s)	474, 1234
BisI	GCNGC	8 cut(s)	153, 202, 247, 325, 440, 765, 768, 1445
BlsI	GCNGC	8 cut(s)	154, 203, 248, 326, 441, 766, 769, 1446
BmcAI	AGTACT	1 cut(s)	38
Bme1390I	CCNGG	5 cut(s)	159, 379, 446, 2351, 2352
Bme18I	GGWCC	5 cut(s)	226, 279, 1141, 1586, 1791
BmeT110I	CYCGRG	1 cut(s)	2350
BmgT120I	GGNCC	6 cut(s)	226, 279, 1141, 1586, 1791, 2348
BmiI	GGNNCC	7 cut(s)	228, 250, 358, 376, 450, 609, 1792
BmrFI	CCNGG	5 cut(s)	159, 379, 446, 2351, 2352
BpiI	GAAGAC	1 cut(s)	1686
BpmI	CTGGAG	2 cut(s)	77, 2499
Bpu10I	CCTNAGC	1 cut(s)	299
BpuEI	CTTGAG	2 cut(s)	500, 2210
BpuMI	CCSGG	3 cut(s)	159, 2351, 2352
BsaBI	GATNNNNATC	1 cut(s)	173
BsaHI	GRCGYC	3 cut(s)	102, 216, 249
BsaJI	CCNNGG	4 cut(s)	157, 453, 671, 2350
BsaWI	WCCGGW	1 cut(s)	1143
BsaXI	ACNNNNNCTCC	6 cut(s)	311, 341, 380, 410, 2322, 2352
Bsc4I	CCNNNNNNNGG	4 cut(s)	165, 245, 300, 368
Bse118I	RCCGGY	1 cut(s)	2131
Bse1I	ACTGG	3 cut(s)	1335, 1935, 2072
Bse21I	CCTNAGG	1 cut(s)	1320
Bse3DI	GCAATG	2 cut(s)	1179, 2103
Bse8I	GATNNNNATC	1 cut(s)	173
BseAI	TCCGGA	1 cut(s)	1143
BseBI	CCWGG	2 cut(s)	379, 446
BseDI	CCNNGG	4 cut(s)	157, 453, 671, 2350
BseGI	GGATG	7 cut(s)	174, 691, 1039, 1164, 1489, 2044, 2392
BseJI	GATNNNNATC	1 cut(s)	173
BseLI	CCNNNNNNNGG	4 cut(s)	165, 245, 300, 368
BseMI	GCAATG	2 cut(s)	1179, 2103
BseMII	CTCAG	7 cut(s)	290, 777, 972, 1145, 1311, 1607, 1914
BseNI	ACTGG	3 cut(s)	1335, 1935, 2072
BseRI	GAGGAG	5 cut(s)	80, 115, 316, 893, 1613
BseSI	GKGCMC	1 cut(s)	461
BseXI	GCAGC	5 cut(s)	213, 311, 451, 776, 1456
BsgI	GTGCAG	1 cut(s)	974
Bsh1236I	CGCG	2 cut(s)	189, 246
Bsh1285I	CGRYCG	1 cut(s)	2132
BshFI	GGCC	5 cut(s)	434, 968, 2210, 2299, 2349
BshNI	GGYRCC	1 cut(s)	248
BsiEI	CGRYCG	1 cut(s)	2132
BsiHKAI	GWGCWC	2 cut(s)	60, 1132
BsiHKCI	CYCGRG	1 cut(s)	2350
BsiSI	CCGG	4 cut(s)	159, 1144, 2132, 2351
BslFI	GGGAC	3 cut(s)	176, 212, 1832
BslI	CCNNNNNNNGG	4 cut(s)	165, 245, 300, 368
BsmAI	GTCTC	3 cut(s)	572, 732, 978
BsmFI	GGGAC	3 cut(s)	176, 212, 1832
BsnI	GGCC	5 cut(s)	434, 968, 2210, 2299, 2349
BsoBI	CYCGRG	1 cut(s)	2350
Bsp1286I	GDGCHC	3 cut(s)	60, 461, 1132
Bsp13I	TCCGGA	1 cut(s)	1143
Bsp1407I	TGTACA	1 cut(s)	2274
BspANI	GGCC	5 cut(s)	434, 968, 2210, 2299, 2349
BspCNI	CTCAG	7 cut(s)	291, 778, 973, 1144, 1312, 1606, 1915
BspEI	TCCGGA	1 cut(s)	1143
BspFNI	CGCG	2 cut(s)	189, 246
BspHI	TCATGA	2 cut(s)	349, 1804
BspLI	GGNNCC	7 cut(s)	228, 250, 358, 376, 450, 609, 1792
BspMAI	CTGCAG	1 cut(s)	766
BspPI	GGATC	4 cut(s)	617, 706, 867, 2482
BspQI	GCTCTTC	2 cut(s)	1059, 1380
BspT107I	GGYRCC	1 cut(s)	248
BsrBI	CCGCTC	2 cut(s)	69, 1802
BsrDI	GCAATG	2 cut(s)	1179, 2103
BsrFI	RCCGGY	1 cut(s)	2131
BsrGI	TGTACA	1 cut(s)	2274
BsrI	ACTGG	3 cut(s)	1335, 1935, 2072
BssAI	RCCGGY	1 cut(s)	2131
BssECI	CCNNGG	4 cut(s)	157, 453, 671, 2350
BssNI	GRCGYC	3 cut(s)	102, 216, 249
BssT1I	CCWWGG	1 cut(s)	453
Bst2UI	CCWGG	2 cut(s)	379, 446
Bst4CI	ACNGT	5 cut(s)	543, 719, 994, 1493, 1747
Bst6I	CTCTTC	5 cut(s)	1059, 1380, 1458, 1701, 2079
BstACI	GRCGYC	3 cut(s)	102, 216, 249
BstAUI	TGTACA	1 cut(s)	2274
BstC8I	GCNNGC	5 cut(s)	432, 1128, 2021, 2258, 2531
BstF5I	GGATG	7 cut(s)	174, 691, 1039, 1164, 1489, 2044, 2392
BstFNI	CGCG	2 cut(s)	189, 246
BstH2I	RGCGCY	1 cut(s)	252
BstHHI	GCGC	5 cut(s)	152, 189, 251, 1089, 2107
BstMAI	GTCTC	3 cut(s)	572, 732, 978
BstMCI	CGRYCG	1 cut(s)	2132
BstMWI	GCNNNNNNNGC	9 cut(s)	94, 304, 436, 1450, 1729, 1823, 2102, 2132, 2296
BstNI	CCWGG	2 cut(s)	379, 446
BstNSI	RCATGY	3 cut(s)	1938, 2005, 2533
BstSCI	CCNGG	5 cut(s)	157, 377, 444, 2349, 2350
BstSFI	CTRYAG	3 cut(s)	762, 1644, 1912
BstSLI	GKGCMC	1 cut(s)	461
BstUI	CGCG	2 cut(s)	189, 246
BstV1I	GCAGC	5 cut(s)	213, 311, 451, 776, 1456
BstV2I	GAAGAC	1 cut(s)	1686
BstX2I	RGATCY	3 cut(s)	474, 1234, 2474
BstYI	RGATCY	3 cut(s)	474, 1234, 2474
Bsu36I	CCTNAGG	1 cut(s)	1320
BsuI	GTATCC	1 cut(s)	1900
BsuRI	GGCC	5 cut(s)	434, 968, 2210, 2299, 2349
BtgZI	GCGATG	1 cut(s)	217
BtsCI	GGATG	7 cut(s)	174, 691, 1039, 1164, 1489, 2044, 2392
BtsI	GCAGTG	1 cut(s)	2437
BtsIMutI	CAGTG	4 cut(s)	1254, 1342, 2079, 2437
Cac8I	GCNNGC	5 cut(s)	432, 1128, 2021, 2258, 2531
CaiI	CAGNNNCTG	3 cut(s)	445, 770, 1643
CciI	TCATGA	2 cut(s)	349, 1804
CfoI	GCGC	5 cut(s)	152, 189, 251, 1089, 2107
Cfr10I	RCCGGY	1 cut(s)	2131
Cfr13I	GGNCC	6 cut(s)	226, 279, 1141, 1586, 1791, 2348
Cfr9I	CCCGGG	1 cut(s)	2350
CseI	GACGC	4 cut(s)	110, 178, 205, 277
Csp6I	GTAC	6 cut(s)	37, 601, 689, 1999, 2275, 2508
CspCI	CAANNNNNGTGG	4 cut(s)	352, 387, 2124, 2159
CviAII	CATG	9 cut(s)	81, 350, 863, 1805, 1935, 2002, 2179, 2530, 2534
CviQI	GTAC	6 cut(s)	37, 601, 689, 1999, 2275, 2508
DinI	GGCGCC	1 cut(s)	250
DraI	TTTAAA	1 cut(s)	655
EaeI	YGGCCR	1 cut(s)	432
Eam1104I	CTCTTC	5 cut(s)	1059, 1380, 1458, 1701, 2079
EarI	CTCTTC	5 cut(s)	1059, 1380, 1458, 1701, 2079
EciI	GGCGGA	1 cut(s)	319
Eco130I	CCWWGG	1 cut(s)	453
Eco147I	AGGCCT	2 cut(s)	968, 2299
Eco32I	GATATC	1 cut(s)	2236
Eco47I	GGWCC	5 cut(s)	226, 279, 1141, 1586, 1791
Eco57I	CTGAAG	2 cut(s)	463, 2475
Eco81I	CCTNAGG	1 cut(s)	1320
Eco88I	CYCGRG	1 cut(s)	2350
EcoRII	CCWGG	2 cut(s)	377, 444
EcoRV	GATATC	1 cut(s)	2236
EcoT14I	CCWWGG	1 cut(s)	453
EcoT22I	ATGCAT	1 cut(s)	2535
EgeI	GGCGCC	1 cut(s)	250
EheI	GGCGCC	1 cut(s)	250
ErhI	CCWWGG	1 cut(s)	453
FaeI	CATG	9 cut(s)	84, 353, 866, 1808, 1938, 2005, 2182, 2533, 2537
FalI	AAGNNNNNCTT	2 cut(s)	98, 130
FaqI	GGGAC	3 cut(s)	176, 212, 1832
FatI	CATG	9 cut(s)	80, 349, 862, 1804, 1934, 2001, 2178, 2529, 2533
FauI	CCCGC	2 cut(s)	942, 2163
FbaI	TGATCA	1 cut(s)	1540
FblI	GTMKAC	2 cut(s)	219, 1344
Fnu4HI	GCNGC	8 cut(s)	153, 202, 247, 325, 440, 765, 768, 1445
FokI	GGATG	7 cut(s)	181, 698, 1046, 1171, 1496, 2051, 2399
Fsp4HI	GCNGC	8 cut(s)	153, 202, 247, 325, 440, 765, 768, 1445
FspBI	CTAG	2 cut(s)	558, 1394
GlaI	GCGC	5 cut(s)	151, 188, 250, 1088, 2106
GluI	GCNGC	8 cut(s)	153, 202, 247, 325, 440, 765, 768, 1445
GsuI	CTGGAG	2 cut(s)	77, 2499
HaeII	RGCGCY	1 cut(s)	252
HaeIII	GGCC	5 cut(s)	434, 968, 2210, 2299, 2349
HapII	CCGG	4 cut(s)	159, 1144, 2132, 2351
HgaI	GACGC	4 cut(s)	110, 178, 205, 277
HhaI	GCGC	5 cut(s)	152, 189, 251, 1089, 2107
Hin1I	GRCGYC	3 cut(s)	102, 216, 249
Hin1II	CATG	9 cut(s)	84, 353, 866, 1808, 1938, 2005, 2182, 2533, 2537
Hin6I	GCGC	5 cut(s)	150, 187, 249, 1087, 2105
HinP1I	GCGC	5 cut(s)	150, 187, 249, 1087, 2105
HincII	GTYRAC	2 cut(s)	220, 1489
HindII	GTYRAC	2 cut(s)	220, 1489
HindIII	AAGCTT	2 cut(s)	1401, 2186
HpaII	CCGG	4 cut(s)	159, 1144, 2132, 2351
HphI	GGTGA	4 cut(s)	22, 436, 460, 1505
Hpy166II	GTNNAC	3 cut(s)	220, 1345, 1489
Hpy8I	GTNNAC	3 cut(s)	220, 1345, 1489
HpyCH4III	ACNGT	5 cut(s)	543, 719, 994, 1493, 1747
HpyCH4IV	ACGT	3 cut(s)	366, 395, 1360
HpyF10VI	GCNNNNNNNGC	9 cut(s)	94, 304, 436, 1450, 1729, 1823, 2102, 2132, 2296
HpySE526I	ACGT	3 cut(s)	366, 395, 1360
Hsp92I	GRCGYC	3 cut(s)	102, 216, 249
Hsp92II	CATG	9 cut(s)	84, 353, 866, 1808, 1938, 2005, 2182, 2533, 2537
HspAI	GCGC	5 cut(s)	150, 187, 249, 1087, 2105
KasI	GGCGCC	1 cut(s)	248
Kpn2I	TCCGGA	1 cut(s)	1143
Ksp22I	TGATCA	1 cut(s)	1540
LguI	GCTCTTC	2 cut(s)	1059, 1380
Lsp1109I	GCAGC	5 cut(s)	213, 311, 451, 776, 1456
MaeI	CTAG	2 cut(s)	558, 1394
MaeII	ACGT	3 cut(s)	366, 395, 1360
MaeIII	GTNAC	9 cut(s)	466, 483, 814, 838, 994, 1417, 1603, 2074, 2408
MbiI	CCGCTC	2 cut(s)	69, 1802
MfeI	CAATTG	1 cut(s)	2270
MflI	RGATCY	3 cut(s)	474, 1234, 2474
MhlI	GDGCHC	3 cut(s)	60, 461, 1132
MlsI	TGGCCA	1 cut(s)	434
MluCI	AATT	5 cut(s)	744, 1612, 1712, 2118, 2270
MluNI	TGGCCA	1 cut(s)	434
Mly113I	GGCGCC	1 cut(s)	249
MlyI	GAGTC	4 cut(s)	413, 799, 1340, 1901
MmeI	TCCRAC	4 cut(s)	146, 366, 1675, 1875
Mox20I	TGGCCA	1 cut(s)	434
Mph1103I	ATGCAT	1 cut(s)	2535
MroI	TCCGGA	1 cut(s)	1143
MroXI	GAANNNNTTC	1 cut(s)	1389
MscI	TGGCCA	1 cut(s)	434
MseI	TTAA	4 cut(s)	597, 654, 1752, 2112
MslI	CAYNNNNRTG	1 cut(s)	720
Msp20I	TGGCCA	1 cut(s)	434
MspA1I	CMGCKG	1 cut(s)	770
MspI	CCGG	4 cut(s)	159, 1144, 2132, 2351
MspR9I	CCNGG	5 cut(s)	159, 379, 446, 2351, 2352
MunI	CAATTG	1 cut(s)	2270
MvaI	CCWGG	2 cut(s)	379, 446
MvnI	CGCG	2 cut(s)	189, 246
MwoI	GCNNNNNNNGC	9 cut(s)	94, 304, 436, 1450, 1729, 1823, 2102, 2132, 2296
NarI	GGCGCC	1 cut(s)	249
NciI	CCSGG	3 cut(s)	159, 2351, 2352
NlaIII	CATG	9 cut(s)	84, 353, 866, 1808, 1938, 2005, 2182, 2533, 2537
NlaIV	GGNNCC	7 cut(s)	228, 250, 358, 376, 450, 609, 1792
NmuCI	GTSAC	5 cut(s)	466, 483, 838, 1417, 2074
NsiI	ATGCAT	1 cut(s)	2535
NspI	RCATGY	3 cut(s)	1938, 2005, 2533
PaeI	GCATGC	1 cut(s)	2533
PagI	TCATGA	2 cut(s)	349, 1804
PceI	AGGCCT	2 cut(s)	968, 2299
PciSI	GCTCTTC	2 cut(s)	1059, 1380
PcsI	WCGNNNNNNNCGW	5 cut(s)	138, 204, 213, 216, 392
PdmI	GAANNNNTTC	1 cut(s)	1389
PfeI	GAWTC	7 cut(s)	1250, 1520, 1552, 1796, 1865, 1877, 2197
PflFI	GACNNNGTC	1 cut(s)	224
PkrI	GCNGC	8 cut(s)	154, 203, 248, 326, 441, 766, 769, 1446
PleI	GAGTC	4 cut(s)	413, 798, 1340, 1900
PluTI	GGCGCC	1 cut(s)	252
PpsI	GAGTC	4 cut(s)	413, 798, 1340, 1900
Psp6I	CCWGG	2 cut(s)	377, 444
PspGI	CCWGG	2 cut(s)	377, 444
PspN4I	GGNNCC	7 cut(s)	228, 250, 358, 376, 450, 609, 1792
PspPI	GGNCC	6 cut(s)	226, 279, 1141, 1586, 1791, 2348
PstI	CTGCAG	1 cut(s)	766
PstNI	CAGNNNCTG	3 cut(s)	445, 770, 1643
PsuI	RGATCY	3 cut(s)	474, 1234, 2474
PsyI	GACNNNGTC	1 cut(s)	224
RsaI	GTAC	6 cut(s)	38, 602, 690, 2000, 2276, 2509
RsaNI	GTAC	6 cut(s)	37, 601, 689, 1999, 2275, 2508
RseI	CAYNNNNRTG	1 cut(s)	720
SalI	GTCGAC	1 cut(s)	218
SapI	GCTCTTC	2 cut(s)	1059, 1380
SaqAI	TTAA	4 cut(s)	597, 654, 1752, 2112
SatI	GCNGC	8 cut(s)	153, 202, 247, 325, 440, 765, 768, 1445
Sau96I	GGNCC	6 cut(s)	226, 279, 1141, 1586, 1791, 2348
ScaI	AGTACT	1 cut(s)	38
SchI	GAGTC	4 cut(s)	413, 799, 1340, 1901
ScrFI	CCNGG	5 cut(s)	159, 379, 446, 2351, 2352
SduI	GDGCHC	3 cut(s)	60, 461, 1132
SfcI	CTRYAG	3 cut(s)	762, 1644, 1912
SfoI	GGCGCC	1 cut(s)	250
SinI	GGWCC	5 cut(s)	226, 279, 1141, 1586, 1791
SmaI	CCCGGG	1 cut(s)	2352
SmiMI	CAYNNNNRTG	1 cut(s)	720
SmlI	CTYRAG	2 cut(s)	515, 2189
SmoI	CTYRAG	2 cut(s)	515, 2189
SphI	GCATGC	1 cut(s)	2533
SrfI	GCCCGGGC	1 cut(s)	2352
Sse9I	AATT	5 cut(s)	744, 1612, 1712, 2118, 2270
SseBI	AGGCCT	2 cut(s)	968, 2299
SspDI	GGCGCC	1 cut(s)	248
SspMI	CTAG	2 cut(s)	558, 1394
StuI	AGGCCT	2 cut(s)	968, 2299
StyD4I	CCNGG	5 cut(s)	157, 377, 444, 2349, 2350
StyI	CCWWGG	1 cut(s)	453
TaaI	ACNGT	5 cut(s)	543, 719, 994, 1493, 1747
TaiI	ACGT	3 cut(s)	369, 398, 1363
TaqI	TCGA	5 cut(s)	99, 141, 219, 663, 2161
TaqII	GACCGA	1 cut(s)	296
TasI	AATT	5 cut(s)	744, 1612, 1712, 2118, 2270
TatI	WGTACW	5 cut(s)	36, 600, 688, 1998, 2274
TauI	GCSGC	3 cut(s)	155, 249, 770
TfiI	GAWTC	7 cut(s)	1250, 1520, 1552, 1796, 1865, 1877, 2197
Tru1I	TTAA	4 cut(s)	597, 654, 1752, 2112
Tru9I	TTAA	4 cut(s)	597, 654, 1752, 2112
TscAI	CASTG	4 cut(s)	1261, 1342, 2079, 2437
TseFI	GTSAC	5 cut(s)	466, 483, 838, 1417, 2074
TseI	GCWGC	5 cut(s)	201, 324, 439, 764, 1444
Tsp45I	GTSAC	5 cut(s)	466, 483, 838, 1417, 2074
TspGWI	ACGGA	2 cut(s)	1371, 2351
TspMI	CCCGGG	1 cut(s)	2350
TspRI	CASTG	4 cut(s)	1261, 1342, 2079, 2437
Tth111I	GACNNNGTC	1 cut(s)	224
VpaK11BI	GGWCC	5 cut(s)	226, 279, 1141, 1586, 1791
XbaI	TCTAGA	1 cut(s)	1393
XceI	RCATGY	3 cut(s)	1938, 2005, 2533
XcmI	CCANNNNNNNNNTGG	2 cut(s)	442, 2032
XmaI	CCCGGG	1 cut(s)	2350
XmiI	GTMKAC	2 cut(s)	219, 1344
XmnI	GAANNNNTTC	1 cut(s)	1389
XspI	CTAG	2 cut(s)	558, 1394
ZrmI	AGTACT	1 cut(s)	38
Zsp2I	ATGCAT	1 cut(s)	2535

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.