Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0013282

Physical Location & Seq

Genomic Coordinates Forward (+)

10168873 .. 10175051

Gene Structure 6179 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1073000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2586 bp

ATGGGAAAGGTGAAGGGCAAGCACCGTTTGGACAAGTACTACAAACTAGCCAAAGAGCATGGCTACCGCTCCCGAGCTTCATGGAAGCTTGCCCAGCTCGACACCAAGCACCAATTCCTTCACTCTTCTCACGCCGTCCTCGACCTCTGCGCCGCGCCCGGCGGTTGGATGCAAGTCGCCGTGCAGCGCGTTCCCGTCGGCAGCCTCGTGGTCGGCGTCGACTTGGTCCCTATCGCCCCTGTCCGCGGCGCCTTCTCGATCCAACAGGACATCACCAAGCCAGAGTGCACGGCCAGGCTCAGAAAGCTGATGAAGGAGAATGGCTGTTCGGCTTTTGATTTGGTGCTTCATGATGGGTCGCCCAATGTCGGCGGCGCGTGGTCTTCGGAGGCCACTGCTCAGAATGCTTTGGTCGTTGATGCGGTGAAGTTGGCTACTCAGCTTTTGGCTCCCAAAGGAACATTTATTACTAAAATCTTCAGGTCGCAAGATTATAATGCTGTCTACTATTGTATGAAGGAGTTATTTGAGAAGGTTGAACAGGATAAACCAGCAGCTAGTCGTAGCTCATCTGCGGAGACATATCTTTTGGGTTTTAAGTACAAGGCTCCTGCAAAGATTGATCCGCGTCTACTTGATGTGAAACATCTATTTAAGTCTGTAGAACCTCAGAAGAAGGTGGTAGATGTACTAAGAGGGACAAAGCAAAAAAGACACCGTGATGGGTATGAAGATGGAGACACAATTTTGAGAAAAGTGTCTTCTGCTGCTGCTTTCATTTGGTCTGATGTTCCTCTCGATATCCTTGGTTCTACTACTTCCATAAGCTTTGACGATGAGGCATCTTTGCCCATCAAGGGTCATGCTTTAACAACAGAAGAGGTTAAAACTCTGTGTGATGATCTGCGTGTTTTGGGGAAGCAAGATTTCAAGTACCTTTTGAAGTGGCGGATGCAGATAAAAAAGGCCTTATCTCCGTCCCAGAAGGCTGATGCTTCTACTACAGATGTTGAAAAGGAGGAAAATGAGGAGGATGATGAAGACAAAATACTCAATGAAATGGAGGAGCTGACGTATGCTATGGAGCGCAAGAAGAAACGAACAAAGAAGCTTCTAGCAAAGAGACGAGCTCAAGACAAGGTACGGAAGGCAACAGGGATGCAAATAGATGCGCTACAAGATGGTTATACTGACAATGAGTTGTTTTCTCTCACTTCCATCAAGGGTAAGAATGATCTGGTAGTGGTTGATTCCACTGAATATGATGGTGAGAATGGTGACCTTGGAGATACTGAAAATGAGGAAACCCATGAGAATACCCAAGAAACATCATCCAGTGATATGGACTCTGATGATGAGCGCAGAAGATATGATGCACAAATGGAAGATCTTTTCGATAAGGCCTATGAACAGTACACCTCCAAAAAGGAGGGAAGTGCAAAGCAGCGGAAACGTTTAAAACAAGCACATTCTGAGGACGCTCAACTTTTGGAGGATGTTGATGGCAGTGATATAGTTCAGTCTGATTATGATTCAGACAAAGACCAGGGTATTCAGGAATCAAATCCTCTCTTGGAATCACTTGATGAGGGGGACGGACCTACCCAAGAGGAAATTACAAATAACTGGTTTAGTCAGGATATCTTTGCCGAAGCTGTAGAGCAGGGGGATTTGGATAACTCTGGTAGTGAAAATGAAATGCAGGTTGAAAAGCGGGAGAAACTTCCCGTGCTAGAAAAGACCAATGAAAAGACTGCAATTCAGAATGGTAAGGAAAAGACTGCAATTCAGAATGCTAAGAAAAATACTGGAGATGATGCTGCAGGGACCAAAGGCCATAAACTTCAAGCTTCAAAGGCTGACGATGATTTTGAGATTGTCCCTGCTCCGGGTACAGATTCAAGTGATGACTCATCATCGGATGAATCCGAGGATATGGATATAGATAAAAAAGCCGAGATACTGGCGTGTGCTAAGAAAATGCTGAGGAAAAAACCAAGGCAACAGATGCTTGATGATGCCTATAACAAGTACATGTTTGATGATGATGGCTTGCCCAGATGGTTTTTAGATGAGGAGAAGAGGCACCGTCAACCAATTAAACCTGTGACCAAAGAAGAGATTAATGCCATGAAAGCACAATTTAAAGAAATTGATGCCCGTCCTGCAAAGAAGGTGGCAGAGGCTAAAGCTCGAAAGAAGCGAGTTGCCTTTAGGAAGCTCGAGAAGGTTCGGAAGAAGGCAAACACAATATCTGACCAGCCAGACATCTCTGATCGTTCAAAGAGGAAGCAAATTGAACAAATGTATAAGAAGGCTGCGCCTAAAAGGCCTCAAAAGGAGTATGTGGTTGCAAAGAAGGGAGTTCAAGTCAGGGTTGGCAAGGGTAAAGTCCGTGTGGATAGACGGATGAAAAAGGATGCAAGGGGTAGTGGGCCGGGTAAAAGGCCTGGAAAAGGCAATTCGAAGAAAGGAAAAACTGCTGCCAAGGGTGAGAGAGGAGGCAAGGGTGCCGGAAAGGGTGACAGAGGCAAGGGCCCCGGAAAGGCTTCAGGAAAGCCAACAGGCAGGAAGGGTAGGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000460 GO:0000463 GO:0000466 GO:0000470 GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008173 GO:0008649 GO:0008650 GO:0008757 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016435 GO:0016436 GO:0016740 GO:0016741 GO:0022613 GO:0030684 GO:0030687 GO:0031167 GO:0031974 GO:0031981 GO:0032259 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042273 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:0140102 GO:1901360 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3381 FtsJ Spb1_C

Protein Analysis

862

Amino Acids

96.35

Weight (kDa)

8.93

Isoelectric Point (pI)

39.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FtsJ	PF01728	22 - 201	1.1e-47	FtsJ-like methyltransferase
DUF3381	PF11861	234 - 382	7.4e-44	Ribosomal RNA methyltransferase Spb1, DUF3381
Spb1_C	PF07780	618 - 811	5.9e-60	Spb1 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013282)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25730
fragaria_vesca	FvH4_2g09160
malus_domestica	MD05G1064300.v1.1 MD10G1073000.v1.1
prunus_persica	Prupe.8G094400_v2.0.a1
pyrus_communis	pycom05g05540 pycom10g05760
rosa_chinensis	RchiOBHm_Chr6g0265021
rosa_laevigata	RLG00000014215 RLG00000014220
rosa_multiflora	Rmu_sc0002934.1_g000022
rosa_roxburghii	Rroxscaffold_175G00432100
rosa_rugosa	Rorug06G0013800
rosa_samantha	Rh6AG136200 Rh6BG133000 Rh6CG131600 Rh6DG118800
rosa_wichuraiana	Rw6G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	495
Acc36I	ACCTGC	1 cut(s)	1693
AccB1I	GGYRCC	3 cut(s)	248, 2085, 2510
AccBSI	CCGCTC	1 cut(s)	69
AccI	GTMKAC	3 cut(s)	219, 504, 631
AccII	CGCG	5 cut(s)	155, 189, 246, 377, 628
AclI	AACGTT	1 cut(s)	1453
AclWI	GGATC	2 cut(s)	253, 617
AcoI	YGGCCR	1 cut(s)	291
AcuI	CTGAAG	2 cut(s)	463, 2535
AcyI	GRCGYC	2 cut(s)	216, 249
AfaI	GTAC	8 cut(s)	38, 602, 690, 935, 1143, 1415, 1894, 2033
AfiI	CCNNNNNNNGG	8 cut(s)	165, 245, 368, 857, 1888, 1889, 2456, 2545
AflIII	ACRYGT	1 cut(s)	2034
AjnI	CCWGG	3 cut(s)	293, 1545, 2449
AjuI	GAANNNNNNNTTGG	2 cut(s)	98, 130
Alw21I	GWGCWC	2 cut(s)	290, 1132
Alw26I	GTCTC	3 cut(s)	572, 732, 1117
Alw44I	GTGCAC	1 cut(s)	286
AlwI	GGATC	2 cut(s)	253, 617
Ama87I	CYCGRG	2 cut(s)	72, 2222
AoxI	GGCC	9 cut(s)	291, 390, 966, 1401, 1834, 2330, 2435, 2447, 2536
ApaI	GGGCCC	1 cut(s)	2540
ApaLI	GTGCAC	1 cut(s)	286
ApeKI	GCWGC	9 cut(s)	184, 201, 554, 767, 770, 1444, 1820, 2318, 2483
ArsI	GACNNNNNNTTYG	4 cut(s)	571, 603, 1853, 1885
AseI	ATTAAT	1 cut(s)	2124
Asp700I	GAANNNNTTC	1 cut(s)	2548
AspLEI	GCGC	9 cut(s)	152, 157, 189, 251, 377, 1089, 1174, 1362, 2323
AspS9I	GGNCC	6 cut(s)	226, 1598, 1827, 2435, 2536, 2537
AsuC2I	CCSGG	4 cut(s)	159, 1890, 2439, 2541
AsuHPI	GGTGA	7 cut(s)	22, 265, 436, 1280, 1289, 2504, 2534
AsuII	TTCGAA	1 cut(s)	2465
AvaI	CYCGRG	2 cut(s)	72, 2222
AvaII	GGWCC	3 cut(s)	226, 1598, 1827
BaeGI	GKGCMC	2 cut(s)	290, 2540
BaeI	ACNNNNGTAYC	2 cut(s)	1952, 1985
BanI	GGYRCC	3 cut(s)	248, 2085, 2510
BanII	GRGCYC	2 cut(s)	1132, 2540
BarI	GAAGNNNNNNTAC	2 cut(s)	1032, 1064
BauI	CACGAG	1 cut(s)	206
BbsI	GAAGAC	3 cut(s)	375, 753, 1047
Bbv12I	GWGCWC	2 cut(s)	290, 1132
BbvCI	CCTCAGC	1 cut(s)	1985
BbvI	GCAGC	9 cut(s)	196, 213, 566, 754, 757, 1456, 1807, 2305, 2470
BceAI	ACGGC	3 cut(s)	119, 164, 306
BciT130I	CCWGG	3 cut(s)	295, 1547, 2451
BcnI	CCSGG	4 cut(s)	159, 1890, 2439, 2541
BcoDI	GTCTC	3 cut(s)	572, 732, 1117
BfaI	CTAG	4 cut(s)	47, 558, 1115, 1733
BfmI	CTRYAG	4 cut(s)	660, 1002, 1656, 1821
BfoI	RGCGCY	1 cut(s)	252
BfuAI	ACCTGC	1 cut(s)	1693
BglI	GCCNNNNNGGC	1 cut(s)	2329
BglII	AGATCT	1 cut(s)	1387
BmcAI	AGTACT	1 cut(s)	38
Bme1390I	CCNGG	7 cut(s)	159, 295, 1547, 1890, 2439, 2451, 2541
Bme18I	GGWCC	3 cut(s)	226, 1598, 1827
BmeT110I	CYCGRG	2 cut(s)	72, 2222
BmgT120I	GGNCC	6 cut(s)	226, 1598, 1827, 2435, 2536, 2537
BmiI	GGNNCC	9 cut(s)	228, 250, 450, 609, 1828, 2087, 2512, 2538, 2539
BmrFI	CCNGG	7 cut(s)	159, 295, 1547, 1890, 2439, 2451, 2541
BpiI	GAAGAC	3 cut(s)	375, 753, 1047
BplI	GAGNNNNNCTC	2 cut(s)	1114, 1146
BpmI	CTGGAG	1 cut(s)	1830
Bpu10I	CCTNAGC	1 cut(s)	1985
Bpu14I	TTCGAA	1 cut(s)	2465
BpuEI	CTTGAG	1 cut(s)	1116
BpuMI	CCSGG	4 cut(s)	159, 1890, 2439, 2541
BsaHI	GRCGYC	2 cut(s)	216, 249
BsaJI	CCNNGG	8 cut(s)	244, 805, 1282, 1546, 1929, 1997, 2487, 2539
BsaXI	ACNNNNNCTCC	4 cut(s)	1058, 1088, 2355, 2385
Bsc4I	CCNNNNNNNGG	8 cut(s)	165, 245, 368, 857, 1888, 1889, 2456, 2545
Bse1I	ACTGG	4 cut(s)	1335, 1631, 1813, 1968
BseBI	CCWGG	3 cut(s)	295, 1547, 2451
BseDI	CCNNGG	8 cut(s)	244, 805, 1282, 1546, 1929, 1997, 2487, 2539
BseGI	GGATG	9 cut(s)	174, 957, 1039, 1164, 1331, 1501, 1927, 2415, 2425
BseLI	CCNNNNNNNGG	8 cut(s)	165, 245, 368, 857, 1888, 1889, 2456, 2545
BseMII	CTCAG	6 cut(s)	313, 413, 452, 683, 1464, 1976
BseNI	ACTGG	4 cut(s)	1335, 1631, 1813, 1968
BseRI	GAGGAG	4 cut(s)	1043, 1079, 2090, 2514
BseSI	GKGCMC	2 cut(s)	290, 2540
BseXI	GCAGC	9 cut(s)	196, 213, 566, 754, 757, 1456, 1807, 2305, 2470
BseYI	CCCAGC	1 cut(s)	93
BsgI	GTGCAG	1 cut(s)	203
Bsh1236I	CGCG	5 cut(s)	155, 189, 246, 377, 628
BshFI	GGCC	9 cut(s)	293, 392, 968, 1403, 1836, 2332, 2437, 2449, 2538
BshNI	GGYRCC	3 cut(s)	248, 2085, 2510
BsiHKAI	GWGCWC	2 cut(s)	290, 1132
BsiHKCI	CYCGRG	2 cut(s)	72, 2222
BsiSI	CCGG	5 cut(s)	159, 1889, 2438, 2514, 2541
BslFI	GGGAC	6 cut(s)	212, 712, 964, 1607, 1840, 1865
BslI	CCNNNNNNNGG	8 cut(s)	165, 245, 368, 857, 1888, 1889, 2456, 2545
BsmAI	GTCTC	3 cut(s)	572, 732, 1117
BsmBI	CGTCTC	1 cut(s)	1117
BsmFI	GGGAC	6 cut(s)	212, 712, 964, 1607, 1840, 1865
BsmI	GAATGC	2 cut(s)	409, 1798
BsnI	GGCC	9 cut(s)	293, 392, 968, 1403, 1836, 2332, 2437, 2449, 2538
BsoBI	CYCGRG	2 cut(s)	72, 2222
Bsp119I	TTCGAA	1 cut(s)	2465
Bsp120I	GGGCCC	1 cut(s)	2536
Bsp1286I	GDGCHC	3 cut(s)	290, 1132, 2540
Bsp143I	GATC	6 cut(s)	258, 622, 901, 1234, 1387, 2275
BspANI	GGCC	9 cut(s)	293, 392, 968, 1403, 1836, 2332, 2437, 2449, 2538
BspCNI	CTCAG	6 cut(s)	312, 412, 451, 682, 1465, 1977
BspFNI	CGCG	5 cut(s)	155, 189, 246, 377, 628
BspHI	TCATGA	1 cut(s)	349
BspLI	GGNNCC	9 cut(s)	228, 250, 450, 609, 1828, 2087, 2512, 2538, 2539
BspMAI	CTGCAG	1 cut(s)	1825
BspMI	ACCTGC	1 cut(s)	1693
BspPI	GGATC	2 cut(s)	253, 617
BspT104I	TTCGAA	1 cut(s)	2465
BspT107I	GGYRCC	3 cut(s)	248, 2085, 2510
BsrBI	CCGCTC	1 cut(s)	69
BsrI	ACTGG	4 cut(s)	1335, 1631, 1813, 1968
BssECI	CCNNGG	8 cut(s)	244, 805, 1282, 1546, 1929, 1997, 2487, 2539
BssMI	GATC	6 cut(s)	258, 622, 901, 1234, 1387, 2275
BssNI	GRCGYC	2 cut(s)	216, 249
BssSI	CACGAG	1 cut(s)	206
BssT1I	CCWWGG	4 cut(s)	805, 1282, 1997, 2487
Bst2BI	CACGAG	1 cut(s)	206
Bst2UI	CCWGG	3 cut(s)	295, 1547, 2451
Bst4CI	ACNGT	4 cut(s)	26, 719, 1413, 2090
Bst6I	CTCTTC	4 cut(s)	130, 873, 2075, 2112
BstACI	GRCGYC	2 cut(s)	216, 249
BstAPI	GCANNNNNTGC	1 cut(s)	2008
BstBI	TTCGAA	1 cut(s)	2465
BstC8I	GCNNGC	3 cut(s)	20, 90, 2054
BstDEI	CTNAG	9 cut(s)	299, 399, 438, 669, 692, 1473, 1797, 1974, 1985
BstDSI	CCRYGG	1 cut(s)	244
BstEII	GGTNACC	1 cut(s)	1277
BstENI	CCTNNNNNAGG	1 cut(s)	2454
BstF5I	GGATG	9 cut(s)	174, 957, 1039, 1164, 1331, 1501, 1927, 2415, 2425
BstFNI	CGCG	5 cut(s)	155, 189, 246, 377, 628
BstH2I	RGCGCY	1 cut(s)	252
BstHHI	GCGC	9 cut(s)	152, 157, 189, 251, 377, 1089, 1174, 1362, 2323
BstKTI	GATC	6 cut(s)	261, 625, 904, 1237, 1390, 2278
BstMAI	GTCTC	3 cut(s)	572, 732, 1117
BstMBI	GATC	6 cut(s)	258, 622, 901, 1234, 1387, 2275
BstMWI	GCNNNNNNNGC	8 cut(s)	94, 304, 404, 1856, 2008, 2135, 2165, 2329
BstNI	CCWGG	3 cut(s)	295, 1547, 2451
BstNSI	RCATGY	1 cut(s)	2038
BstPI	GGTNACC	1 cut(s)	1277
BstSCI	CCNGG	7 cut(s)	157, 293, 1545, 1888, 2437, 2449, 2539
BstSFI	CTRYAG	4 cut(s)	660, 1002, 1656, 1821
BstSLI	GKGCMC	2 cut(s)	290, 2540
BstUI	CGCG	5 cut(s)	155, 189, 246, 377, 628
BstV1I	GCAGC	9 cut(s)	196, 213, 566, 754, 757, 1456, 1807, 2305, 2470
BstV2I	GAAGAC	3 cut(s)	375, 753, 1047
BstX2I	RGATCY	1 cut(s)	1387
BstXI	CCANNNNNNTGG	1 cut(s)	1342
BstYI	RGATCY	1 cut(s)	1387
BsuRI	GGCC	9 cut(s)	293, 392, 968, 1403, 1836, 2332, 2437, 2449, 2538
BtgI	CCRYGG	1 cut(s)	244
BtsCI	GGATG	9 cut(s)	174, 957, 1039, 1164, 1331, 1501, 1927, 2415, 2425
BtsI	GCAGTG	2 cut(s)	393, 1513
BtsIMutI	CAGTG	4 cut(s)	393, 1254, 1342, 1513
BveI	ACCTGC	1 cut(s)	1693
Cac8I	GCNNGC	3 cut(s)	20, 90, 2054
CciI	TCATGA	1 cut(s)	349
CfoI	GCGC	9 cut(s)	152, 157, 189, 251, 377, 1089, 1174, 1362, 2323
Cfr13I	GGNCC	6 cut(s)	226, 1598, 1827, 2435, 2536, 2537
Cfr42I	CCGCGG	1 cut(s)	247
CseI	GACGC	3 cut(s)	205, 617, 1487
Csp6I	GTAC	8 cut(s)	37, 601, 689, 934, 1142, 1414, 1893, 2032
CspCI	CAANNNNNGTGG	2 cut(s)	2157, 2192
CviAII	CATG	7 cut(s)	59, 81, 350, 863, 1310, 2035, 2131
CviQI	GTAC	8 cut(s)	37, 601, 689, 934, 1142, 1414, 1893, 2032
DdeI	CTNAG	9 cut(s)	299, 399, 438, 669, 692, 1473, 1797, 1974, 1985
DinI	GGCGCC	1 cut(s)	250
DpnI	GATC	6 cut(s)	260, 624, 903, 1236, 1389, 2277
DpnII	GATC	6 cut(s)	258, 622, 901, 1234, 1387, 2275
DraI	TTTAAA	2 cut(s)	1458, 2146
EaeI	YGGCCR	1 cut(s)	291
Eam1104I	CTCTTC	4 cut(s)	130, 873, 2075, 2112
EarI	CTCTTC	4 cut(s)	130, 873, 2075, 2112
EciI	GGCGGA	1 cut(s)	964
Ecl136II	GAGCTC	1 cut(s)	1130
Eco130I	CCWWGG	4 cut(s)	805, 1282, 1997, 2487
Eco147I	AGGCCT	4 cut(s)	968, 1403, 2332, 2449
Eco24I	GRGCYC	2 cut(s)	1132, 2540
Eco32I	GATATC	2 cut(s)	802, 1642
Eco47I	GGWCC	3 cut(s)	226, 1598, 1827
Eco53kI	GAGCTC	1 cut(s)	1130
Eco57I	CTGAAG	2 cut(s)	463, 2535
Eco88I	CYCGRG	2 cut(s)	72, 2222
Eco91I	GGTNACC	1 cut(s)	1277
EcoICRI	GAGCTC	1 cut(s)	1130
EcoNI	CCTNNNNNAGG	1 cut(s)	2454
EcoO109I	RGGNCCY	2 cut(s)	2536, 2537
EcoO65I	GGTNACC	1 cut(s)	1277
EcoRII	CCWGG	3 cut(s)	293, 1545, 2449
EcoRV	GATATC	2 cut(s)	802, 1642
EcoT14I	CCWWGG	4 cut(s)	805, 1282, 1997, 2487
EcoT38I	GRGCYC	2 cut(s)	1132, 2540
EgeI	GGCGCC	1 cut(s)	250
EheI	GGCGCC	1 cut(s)	250
ErhI	CCWWGG	4 cut(s)	805, 1282, 1997, 2487
Esp3I	CGTCTC	1 cut(s)	1117
FaeI	CATG	7 cut(s)	62, 84, 353, 866, 1313, 2038, 2134
FaqI	GGGAC	6 cut(s)	212, 712, 964, 1607, 1840, 1865
FatI	CATG	7 cut(s)	58, 80, 349, 862, 1309, 2034, 2130
FauI	CCCGC	1 cut(s)	1707
FblI	GTMKAC	3 cut(s)	219, 504, 631
FokI	GGATG	9 cut(s)	181, 964, 1046, 1171, 1318, 1508, 1934, 2422, 2432
FriOI	GRGCYC	2 cut(s)	1132, 2540
FspBI	CTAG	4 cut(s)	47, 558, 1115, 1733
GlaI	GCGC	9 cut(s)	151, 156, 188, 250, 376, 1088, 1173, 1361, 2322
GsaI	CCCAGC	1 cut(s)	97
GsuI	CTGGAG	1 cut(s)	1830
HaeII	RGCGCY	1 cut(s)	252
HaeIII	GGCC	9 cut(s)	293, 392, 968, 1403, 1836, 2332, 2437, 2449, 2538
HapII	CCGG	5 cut(s)	159, 1889, 2438, 2514, 2541
HgaI	GACGC	3 cut(s)	205, 617, 1487
HhaI	GCGC	9 cut(s)	152, 157, 189, 251, 377, 1089, 1174, 1362, 2323
Hin1I	GRCGYC	2 cut(s)	216, 249
Hin1II	CATG	7 cut(s)	62, 84, 353, 866, 1313, 2038, 2134
Hin6I	GCGC	9 cut(s)	150, 155, 187, 249, 375, 1087, 1172, 1360, 2321
HinP1I	GCGC	9 cut(s)	150, 155, 187, 249, 375, 1087, 1172, 1360, 2321
HincII	GTYRAC	2 cut(s)	220, 2093
HindII	GTYRAC	2 cut(s)	220, 2093
HindIII	AAGCTT	4 cut(s)	86, 826, 1109, 1848
HinfI	GANTC	8 cut(s)	1250, 1346, 1532, 1559, 1577, 1898, 1910, 1925
HpaII	CCGG	5 cut(s)	159, 1889, 2438, 2514, 2541
HphI	GGTGA	7 cut(s)	22, 265, 436, 1280, 1289, 2504, 2534
Hpy166II	GTNNAC	6 cut(s)	220, 288, 505, 632, 1416, 2093
Hpy188III	TCNNGA	9 cut(s)	72, 256, 350, 797, 1133, 1556, 1637, 2224, 2553
Hpy8I	GTNNAC	6 cut(s)	220, 288, 505, 632, 1416, 2093
Hpy99I	CGWCG	2 cut(s)	200, 221
HpyCH4III	ACNGT	4 cut(s)	26, 719, 1413, 2090
HpyCH4IV	ACGT	2 cut(s)	1073, 1453
HpyF10VI	GCNNNNNNNGC	8 cut(s)	94, 304, 404, 1856, 2008, 2135, 2165, 2329
HpyF3I	CTNAG	9 cut(s)	299, 399, 438, 669, 692, 1473, 1797, 1974, 1985
HpySE526I	ACGT	2 cut(s)	1073, 1453
Hsp92I	GRCGYC	2 cut(s)	216, 249
Hsp92II	CATG	7 cut(s)	62, 84, 353, 866, 1313, 2038, 2134
HspAI	GCGC	9 cut(s)	150, 155, 187, 249, 375, 1087, 1172, 1360, 2321
KasI	GGCGCC	1 cut(s)	248
KspI	CCGCGG	1 cut(s)	247
Kzo9I	GATC	6 cut(s)	258, 622, 901, 1234, 1387, 2275
LmnI	GCTCC	6 cut(s)	74, 454, 613, 1066, 1084, 1891
Lsp1109I	GCAGC	9 cut(s)	196, 213, 566, 754, 757, 1456, 1807, 2305, 2470
MaeI	CTAG	4 cut(s)	47, 558, 1115, 1733
MaeII	ACGT	2 cut(s)	1073, 1453
MaeIII	GTNAC	3 cut(s)	1277, 2107, 2522
MalI	GATC	6 cut(s)	260, 624, 903, 1236, 1389, 2277
MbiI	CCGCTC	1 cut(s)	69
MboI	GATC	6 cut(s)	258, 622, 901, 1234, 1387, 2275
MflI	RGATCY	1 cut(s)	1387
MhlI	GDGCHC	3 cut(s)	290, 1132, 2540
Mly113I	GGCGCC	1 cut(s)	249
MlyI	GAGTC	2 cut(s)	1340, 1904
MmeI	TCCRAC	2 cut(s)	146, 286
MroXI	GAANNNNTTC	1 cut(s)	2548
MseI	TTAA	8 cut(s)	597, 654, 869, 885, 1457, 2100, 2124, 2145
MslI	CAYNNNNRTG	1 cut(s)	720
MspA1I	CMGCKG	2 cut(s)	246, 1447
MspI	CCGG	5 cut(s)	159, 1889, 2438, 2514, 2541
MspR9I	CCNGG	7 cut(s)	159, 295, 1547, 1890, 2439, 2451, 2541
MteI	GCGCNGCGC	1 cut(s)	153
Mva1269I	GAATGC	2 cut(s)	409, 1798
MvaI	CCWGG	3 cut(s)	295, 1547, 2451
MvnI	CGCG	5 cut(s)	155, 189, 246, 377, 628
MwoI	GCNNNNNNNGC	8 cut(s)	94, 304, 404, 1856, 2008, 2135, 2165, 2329
NarI	GGCGCC	1 cut(s)	249
NciI	CCSGG	4 cut(s)	159, 1890, 2439, 2541
NdeII	GATC	6 cut(s)	258, 622, 901, 1234, 1387, 2275
NlaIII	CATG	7 cut(s)	62, 84, 353, 866, 1313, 2038, 2134
NlaIV	GGNNCC	9 cut(s)	228, 250, 450, 609, 1828, 2087, 2512, 2538, 2539
NmeAIII	GCCGAG	1 cut(s)	1981
NmuCI	GTSAC	3 cut(s)	1277, 2107, 2522
NspI	RCATGY	1 cut(s)	2038
NspV	TTCGAA	1 cut(s)	2465
PaeR7I	CTCGAG	1 cut(s)	2222
PagI	TCATGA	1 cut(s)	349
PceI	AGGCCT	4 cut(s)	968, 1403, 2332, 2449
PciI	ACATGT	1 cut(s)	2034
PcsI	WCGNNNNNNNCGW	4 cut(s)	138, 204, 213, 2200
PctI	GAATGC	2 cut(s)	409, 1798
PdmI	GAANNNNTTC	1 cut(s)	2548
PfeI	GAWTC	6 cut(s)	1250, 1532, 1559, 1577, 1898, 1925
PflFI	GACNNNGTC	1 cut(s)	224
PleI	GAGTC	2 cut(s)	1340, 1904
PluTI	GGCGCC	1 cut(s)	252
PpsI	GAGTC	2 cut(s)	1340, 1904
PscI	ACATGT	1 cut(s)	2034
PshBI	ATTAAT	1 cut(s)	2124
PsiI	TTATAA	1 cut(s)	495
Psp124BI	GAGCTC	1 cut(s)	1132
Psp1406I	AACGTT	1 cut(s)	1453
Psp6I	CCWGG	3 cut(s)	293, 1545, 2449
PspEI	GGTNACC	1 cut(s)	1277
PspFI	CCCAGC	1 cut(s)	93
PspGI	CCWGG	3 cut(s)	293, 1545, 2449
PspN4I	GGNNCC	9 cut(s)	228, 250, 450, 609, 1828, 2087, 2512, 2538, 2539
PspOMI	GGGCCC	1 cut(s)	2536
PspPI	GGNCC	6 cut(s)	226, 1598, 1827, 2435, 2536, 2537
PsrI	GAACNNNNNNTAC	2 cut(s)	451, 483
PstI	CTGCAG	1 cut(s)	1825
PsuI	RGATCY	1 cut(s)	1387
PsyI	GACNNNGTC	1 cut(s)	224
RsaI	GTAC	8 cut(s)	38, 602, 690, 935, 1143, 1415, 1894, 2033
RsaNI	GTAC	8 cut(s)	37, 601, 689, 934, 1142, 1414, 1893, 2032
RseI	CAYNNNNRTG	1 cut(s)	720
SacI	GAGCTC	1 cut(s)	1132
SacII	CCGCGG	1 cut(s)	247
SalI	GTCGAC	1 cut(s)	218
SaqAI	TTAA	8 cut(s)	597, 654, 869, 885, 1457, 2100, 2124, 2145
Sau3AI	GATC	6 cut(s)	258, 622, 901, 1234, 1387, 2275
Sau96I	GGNCC	6 cut(s)	226, 1598, 1827, 2435, 2536, 2537
ScaI	AGTACT	1 cut(s)	38
SchI	GAGTC	2 cut(s)	1340, 1904
ScrFI	CCNGG	7 cut(s)	159, 295, 1547, 1890, 2439, 2451, 2541
SduI	GDGCHC	3 cut(s)	290, 1132, 2540
SfcI	CTRYAG	4 cut(s)	660, 1002, 1656, 1821
SfoI	GGCGCC	1 cut(s)	250
Sfr274I	CTCGAG	1 cut(s)	2222
Sfr303I	CCGCGG	1 cut(s)	247
SfuI	TTCGAA	1 cut(s)	2465
SgrBI	CCGCGG	1 cut(s)	247
SinI	GGWCC	3 cut(s)	226, 1598, 1827
SlaI	CTCGAG	1 cut(s)	2222
SmiMI	CAYNNNNRTG	1 cut(s)	720
SmlI	CTYRAG	2 cut(s)	1131, 2222
SmoI	CTYRAG	2 cut(s)	1131, 2222
SseBI	AGGCCT	4 cut(s)	968, 1403, 2332, 2449
SspDI	GGCGCC	1 cut(s)	248
SspMI	CTAG	4 cut(s)	47, 558, 1115, 1733
SstI	GAGCTC	1 cut(s)	1132
StuI	AGGCCT	4 cut(s)	968, 1403, 2332, 2449
StyD4I	CCNGG	7 cut(s)	157, 293, 1545, 1888, 2437, 2449, 2539
StyI	CCWWGG	4 cut(s)	805, 1282, 1997, 2487
TaaI	ACNGT	4 cut(s)	26, 719, 1413, 2090
TaiI	ACGT	2 cut(s)	1076, 1456
TaqI	TCGA	9 cut(s)	99, 141, 219, 257, 798, 1395, 2194, 2223, 2465
TatI	WGTACW	5 cut(s)	36, 600, 688, 1413, 2031
TauI	GCSGC	3 cut(s)	155, 249, 375
TfiI	GAWTC	6 cut(s)	1250, 1532, 1559, 1577, 1898, 1925
Tru1I	TTAA	8 cut(s)	597, 654, 869, 885, 1457, 2100, 2124, 2145
Tru9I	TTAA	8 cut(s)	597, 654, 869, 885, 1457, 2100, 2124, 2145
TscAI	CASTG	4 cut(s)	400, 1261, 1342, 1513
TseFI	GTSAC	3 cut(s)	1277, 2107, 2522
TseI	GCWGC	9 cut(s)	184, 201, 554, 767, 770, 1444, 1820, 2318, 2483
Tsp45I	GTSAC	3 cut(s)	1277, 2107, 2522
TspGWI	ACGGA	5 cut(s)	966, 1159, 1611, 2384, 2422
TspRI	CASTG	4 cut(s)	400, 1261, 1342, 1513
Tth111I	GACNNNGTC	1 cut(s)	224
VneI	GTGCAC	1 cut(s)	286
VpaK11BI	GGWCC	3 cut(s)	226, 1598, 1827
VspI	ATTAAT	1 cut(s)	2124
XagI	CCTNNNNNAGG	1 cut(s)	2454
XceI	RCATGY	1 cut(s)	2038
XhoI	CTCGAG	1 cut(s)	2222
XmiI	GTMKAC	3 cut(s)	219, 504, 631
XmnI	GAANNNNTTC	1 cut(s)	2548
XspI	CTAG	4 cut(s)	47, 558, 1115, 1733
ZrmI	AGTACT	1 cut(s)	38

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.