Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0013282

Physical Location & Seq

Genomic Coordinates Forward (+)

11438528 .. 11445023

Gene Structure 6496 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1064300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2583 bp

ATGGGGAAGGTGAAGGGCAAGCACCGTTTGGACAAGTACTACAAACTAGCCAAAGAGCATGGCTACCGCTCCCGAGCTTCATGGAAGCTTGCCCAGCTCGACACCAAGCACCAATTCCTCCACTCTGCTCACGCCGTCCTCGACCTCTGCGCCGCCCCCGGCGGTTGGATGCAAGTCGCTGTGCAGCGCGTGCCCGTCGGCAGCCTCGTGGTCGGCGTCGACCTAGTCCCCATCGCCCCGGTCCGCGGCGCCTTCTCCATCCAGCAGGACATCACCAAGCCCGAGTGCACGGCCAGGCTCAGAAAGCTGATAAAGGAGAATGGGTGTTCGGCTTTCGATTTGGTGCTTCATGATGGGTCGCCTAATGTGGGTGGCGCGTGGTCTTCGGAGGCGACGGCTCAGAACGCTTTGGTCGTTGATGCGGTGAAGTTGGCTACTCAGCTTTTGGCTCCCAAAGGAACATTTATTACTAAGATCTTCAGGTCGCAGGATTATAATGCTGTCTACTATTGTATGAAGGAGCTATTTGAGAAGGTTGAACAGGATAAACCAGCCGCTAGTCGTAGCTCATCTGCTGAGACATATCTTTTGGGTTTTAAGTACAAGGCTCCTGCAAAGATTGATCCACGCCTACTTGACGTGAAGCATCTATTTAAGTCTGTAGAACCTCAGAAGAAGGTGGTAGATGTACTAAGAGGAACAAAGCAAAAAAGACACCGTGATGGGTATGAAGACGGAGACACAATTTTGAGAAAAGTGACTTCTGCTGCTGCTTTCATTTGGTCTGATGTTCCTCTCAATATCCTTGGTTCTACTACATCCCTAAGCTTTGACGATGAGGCATCTTTACTCCTCAAGGATCATGCTTTAACAACAGAAGAGGTTAAAACTCTCTGTGATGATCTGCGTGTTTTGGGGAAGCAAGATTTCAAGTACCTTTTGAAGTGGCGGATGCAGATAAAAAAGGCCTTATCTCCGTCCCATAAGATTGTCGCTTCTACTACAGATGTTGAAAAGGAGGAGAATAAGGAGGATAAGGATGATGATGAAGACAAAATACTCAATGAAATGGAGGAGCTGACGTATGCTATGGAGCGCAAGAAGAAACGAACAAAGAAGCTTCTAGCAAAAAGACGAGCTCAAGACAAGGTACGGAAAGCAACAGGGATGCAAATAGATGCACTGCAAGATGGTTATACTGACAATGAGTTGTTTTCCCTCGCTTCCATTAAGGGTAAGAATGATCTGGTAGCCGTTGATTCCACTGAATATGATGGTGAGAATGGTGACCTTGGAGACGGTGAAAATGAGGAAACCCATGAGAAAACCCAAGAAGCATCATCCAGTGATATGGACACTGAAGATGAACGCAGAAGATATGATGCACAAATGGAAGATCTTTTCGACAAGGCTTATGAACAGTACACGTCCAAAAAGGAGGGAAGTGCAAAGCAGCGGAAACGTTTAAAACAAGCACATTTTGAAGACACTCAACTTTTGAAGGAGGTGGATGGCAGTGATATTGTTCAGTCTGATTATGATTCAGACAAAGGCCAGGGTATCCAAGAATCGAATCCTCTATTGGAAGCACTTGACGAGGGGGATGGACCTACTCAAGAGGAAATTACGAATAACTGGTTTAGTCAGGATATCTTTGCTGAAGCTGTAGAGCAGGGGGATTTGGAGAACTCTGGTAGTGAAGATGAAATGCTGGTTGAAAGGCAGGAGAAATTTCCCGTGCTGGAAAAGGCCAATGAAAAGACTGCAATTCAGAATGGTAAGGAAAATAATGCAATTCACAATGCCAAGAATAAGACTGAAAATCACCATAAACTTCAAGCTTCAAAGGCAGATGATGATTTTCAGATTGTCCCTGCTCCGGGTACAGATTCAAGTGATGACTTGTCATCAGATGAATCCGAGGGTATGGATATAGATAAAAAAGCCGAGATACTGGCGTGTGCAAAGAAAATGCTGAGGAAAAAGCCAAGGGAACAGATGCTTGATGATGCCTGTAACAGGTACATGTTTGATGATGACGGCTTGCCCAGATGGTTTTTAGATGAGGAGAAGAGGCACCGTCAACCAATTAAACCAGTGACCAAAGAAGAGATTAATGCCATGAAAGCACAATTCAAAGAAATTGATGCCCGGCCTGCAAAGAAGGTGGCAGAGGCAAAAGCTCGAAAGAAGCGAGTTGCCTTTAGGAAGCTTGAGAAGGTTCGGAAGAAGGCAAACACAATATCTGACCAGCCAGACATCTCTGATCGTTCAAAGAGGAAGCAAATTGAACAAATGTACAAGAAGGCTGCGCCCAAAAGGCCTCAAAAGGAGTATGTGGTTGCAAAGAAAGGAGTTCAAGTCAGGGTTGGCAAGGGTAAAGTACGTGTGGATCGAAGGATGAAAAAGGATGCAAGGGGTAGTGGACCAGGTAAAAGGCCCGGAAAAGGCAATTCAAAGAAAGGAAAAACTGCTGCCAAGGGTGAGAGAGGAGGCAAGGGTGCCGGAAAGGGTGACAGAGGCAAGGGGCTGGGGAAAGGAAAGGCTTCAGGAAAGCCAACAGGCAGGAAGGGTAGGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000460 GO:0000463 GO:0000466 GO:0000470 GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008173 GO:0008649 GO:0008650 GO:0008757 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016435 GO:0016436 GO:0016740 GO:0016741 GO:0022613 GO:0030684 GO:0030687 GO:0031167 GO:0031974 GO:0031981 GO:0032259 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042273 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:0140102 GO:1901360 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3381 FtsJ Spb1_C

Protein Analysis

861

Amino Acids

96.5

Weight (kDa)

9.08

Isoelectric Point (pI)

38.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FtsJ	PF01728	22 - 201	1.5e-47	FtsJ-like methyltransferase
DUF3381	PF11861	234 - 385	7.1e-43	Ribosomal RNA methyltransferase Spb1, DUF3381
Spb1_C	PF07780	615 - 808	2e-58	Spb1 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013282)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25730
fragaria_vesca	FvH4_2g09160
malus_domestica	MD05G1064300.v1.1 MD10G1073000.v1.1
prunus_persica	Prupe.8G094400_v2.0.a1
pyrus_communis	pycom05g05540 pycom10g05760
rosa_chinensis	RchiOBHm_Chr6g0265021
rosa_laevigata	RLG00000014215 RLG00000014220
rosa_multiflora	Rmu_sc0002934.1_g000022
rosa_roxburghii	Rroxscaffold_175G00432100
rosa_rugosa	Rorug06G0013800
rosa_samantha	Rh6AG136200 Rh6BG133000 Rh6CG131600 Rh6DG118800
rosa_wichuraiana	Rw6G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	495
AccB1I	GGYRCC	3 cut(s)	248, 2076, 2501
AccBSI	CCGCTC	1 cut(s)	69
AccI	GTMKAC	2 cut(s)	219, 504
AccII	CGCG	3 cut(s)	189, 246, 377
AciI	CCGC	9 cut(s)	67, 153, 162, 244, 246, 422, 555, 949, 1456
AclI	AACGTT	1 cut(s)	1462
AclWI	GGATC	3 cut(s)	617, 867, 2400
AcoI	YGGCCR	1 cut(s)	291
AcsI	RAATTY	1 cut(s)	1730
AcuI	CTGAAG	4 cut(s)	463, 1380, 1680, 2532
AcyI	GRCGYC	2 cut(s)	216, 249
AfiI	CCNNNNNNNGG	7 cut(s)	165, 245, 368, 1879, 1880, 2019, 2447
AflIII	ACRYGT	3 cut(s)	1425, 2025, 2386
AjiI	CACGTC	2 cut(s)	640, 1428
AjnI	CCWGG	3 cut(s)	293, 1554, 2428
AjuI	GAANNNNNNNTTGG	4 cut(s)	98, 130, 1565, 1597
Alw21I	GWGCWC	2 cut(s)	290, 1141
Alw26I	GTCTC	3 cut(s)	572, 732, 1290
Alw44I	GTGCAC	1 cut(s)	286
AlwI	GGATC	3 cut(s)	617, 867, 2400
Ama87I	CYCGRG	2 cut(s)	72, 281
AoxI	GGCC	7 cut(s)	291, 966, 1552, 1749, 2153, 2321, 2438
ApaLI	GTGCAC	1 cut(s)	286
ApeKI	GCWGC	7 cut(s)	184, 201, 767, 770, 1453, 2309, 2474
ApoI	RAATTY	1 cut(s)	1730
ArsI	GACNNNNNNTTYG	2 cut(s)	571, 603
AseI	ATTAAT	1 cut(s)	2115
Asp700I	GAANNNNTTC	1 cut(s)	2545
AspLEI	GCGC	6 cut(s)	152, 189, 251, 377, 1098, 2314
AspS9I	GGNCC	4 cut(s)	241, 1607, 2426, 2439
AsuC2I	CCSGG	5 cut(s)	159, 239, 1881, 2152, 2442
AsuHPI	GGTGA	9 cut(s)	22, 265, 436, 1289, 1298, 1313, 1817, 2495, 2525
AvaI	CYCGRG	2 cut(s)	72, 281
AvaII	GGWCC	3 cut(s)	241, 1607, 2426
BaeGI	GKGCMC	2 cut(s)	195, 290
BaeI	ACNNNNGTAYC	2 cut(s)	1943, 1976
BanI	GGYRCC	3 cut(s)	248, 2076, 2501
BanII	GRGCYC	1 cut(s)	1141
BarI	GAAGNNNNNNTAC	2 cut(s)	1041, 1073
BauI	CACGAG	1 cut(s)	206
BbsI	GAAGAC	4 cut(s)	375, 738, 1056, 1491
Bbv12I	GWGCWC	2 cut(s)	290, 1141
BbvCI	CCTCAGC	1 cut(s)	1976
BbvI	GCAGC	7 cut(s)	196, 213, 754, 757, 1465, 2296, 2461
BccI	CCATC	9 cut(s)	239, 266, 347, 716, 1184, 1268, 1505, 1598, 2046
BceAI	ACGGC	5 cut(s)	119, 306, 411, 1238, 2056
BciT130I	CCWGG	3 cut(s)	295, 1556, 2430
BciVI	GTATCC	1 cut(s)	1571
BcnI	CCSGG	5 cut(s)	159, 239, 1881, 2152, 2442
BcoDI	GTCTC	3 cut(s)	572, 732, 1290
BfaI	CTAG	4 cut(s)	47, 224, 558, 1124
BfmI	CTRYAG	3 cut(s)	660, 1002, 1665
BfoI	RGCGCY	1 cut(s)	252
BfuI	GTATCC	1 cut(s)	1571
BglI	GCCNNNNNGGC	1 cut(s)	2320
BglII	AGATCT	2 cut(s)	474, 1396
BmcAI	AGTACT	1 cut(s)	38
Bme1390I	CCNGG	8 cut(s)	159, 239, 295, 1556, 1881, 2152, 2430, 2442
Bme18I	GGWCC	3 cut(s)	241, 1607, 2426
BmeT110I	CYCGRG	2 cut(s)	72, 281
BmgBI	CACGTC	2 cut(s)	640, 1428
BmgT120I	GGNCC	4 cut(s)	241, 1607, 2426, 2439
BmiI	GGNNCC	5 cut(s)	250, 450, 609, 2078, 2503
BmrFI	CCNGG	8 cut(s)	159, 239, 295, 1556, 1881, 2152, 2430, 2442
BpiI	GAAGAC	4 cut(s)	375, 738, 1056, 1491
Bpu10I	CCTNAGC	2 cut(s)	824, 1976
BpuEI	CTTGAG	4 cut(s)	839, 1125, 1599, 2234
BpuMI	CCSGG	5 cut(s)	159, 239, 1881, 2152, 2442
BsaAI	YACGTR	1 cut(s)	2387
BsaHI	GRCGYC	2 cut(s)	216, 249
BsaJI	CCNNGG	9 cut(s)	157, 237, 244, 805, 1291, 1555, 1920, 1988, 2478
BsaXI	ACNNNNNCTCC	6 cut(s)	308, 338, 1067, 1097, 2346, 2376
Bsc4I	CCNNNNNNNGG	7 cut(s)	165, 245, 368, 1879, 1880, 2019, 2447
Bse1I	ACTGG	4 cut(s)	1344, 1640, 1959, 2096
BseBI	CCWGG	3 cut(s)	295, 1556, 2430
BseDI	CCNNGG	9 cut(s)	157, 237, 244, 805, 1291, 1555, 1920, 1988, 2478
BseLI	CCNNNNNNNGG	7 cut(s)	165, 245, 368, 1879, 1880, 2019, 2447
BseMII	CTCAG	6 cut(s)	313, 413, 452, 567, 683, 1967
BseNI	ACTGG	4 cut(s)	1344, 1640, 1959, 2096
BseRI	GAGGAG	5 cut(s)	842, 1034, 1088, 2081, 2505
BseSI	GKGCMC	2 cut(s)	195, 290
BseXI	GCAGC	7 cut(s)	196, 213, 754, 757, 1465, 2296, 2461
BseYI	CCCAGC	2 cut(s)	93, 2530
BsgI	GTGCAG	1 cut(s)	203
Bsh1236I	CGCG	3 cut(s)	189, 246, 377
BshFI	GGCC	7 cut(s)	293, 968, 1554, 1751, 2155, 2323, 2440
BshNI	GGYRCC	3 cut(s)	248, 2076, 2501
BsiHKAI	GWGCWC	2 cut(s)	290, 1141
BsiHKCI	CYCGRG	2 cut(s)	72, 281
BsiSI	CCGG	6 cut(s)	159, 239, 1880, 2152, 2442, 2505
BslFI	GGGAC	3 cut(s)	212, 964, 1856
BslI	CCNNNNNNNGG	7 cut(s)	165, 245, 368, 1879, 1880, 2019, 2447
BsmAI	GTCTC	3 cut(s)	572, 732, 1290
BsmBI	CGTCTC	1 cut(s)	1290
BsmFI	GGGAC	3 cut(s)	212, 964, 1856
BsnI	GGCC	7 cut(s)	293, 968, 1554, 1751, 2155, 2323, 2440
BsoBI	CYCGRG	2 cut(s)	72, 281
Bsp1286I	GDGCHC	3 cut(s)	195, 290, 1141
Bsp1407I	TGTACA	1 cut(s)	2298
Bsp143I	GATC	8 cut(s)	474, 622, 859, 901, 1243, 1396, 2266, 2392
BspACI	CCGC	9 cut(s)	67, 153, 162, 244, 246, 422, 555, 949, 1456
BspANI	GGCC	7 cut(s)	293, 968, 1554, 1751, 2155, 2323, 2440
BspCNI	CTCAG	6 cut(s)	312, 412, 451, 568, 682, 1968
BspFNI	CGCG	3 cut(s)	189, 246, 377
BspHI	TCATGA	1 cut(s)	349
BspLI	GGNNCC	5 cut(s)	250, 450, 609, 2078, 2503
BspPI	GGATC	3 cut(s)	617, 867, 2400
BspT107I	GGYRCC	3 cut(s)	248, 2076, 2501
BsrBI	CCGCTC	1 cut(s)	69
BsrGI	TGTACA	1 cut(s)	2298
BsrI	ACTGG	4 cut(s)	1344, 1640, 1959, 2096
BssECI	CCNNGG	9 cut(s)	157, 237, 244, 805, 1291, 1555, 1920, 1988, 2478
BssMI	GATC	8 cut(s)	474, 622, 859, 901, 1243, 1396, 2266, 2392
BssNI	GRCGYC	2 cut(s)	216, 249
BssSI	CACGAG	1 cut(s)	206
BssT1I	CCWWGG	4 cut(s)	805, 1291, 1988, 2478
Bst2BI	CACGAG	1 cut(s)	206
Bst2UI	CCWGG	3 cut(s)	295, 1556, 2430
Bst4CI	ACNGT	5 cut(s)	26, 719, 1301, 1422, 2081
Bst6I	CTCTTC	3 cut(s)	873, 2066, 2103
BstACI	GRCGYC	2 cut(s)	216, 249
BstAPI	GCANNNNNTGC	1 cut(s)	190
BstAUI	TGTACA	1 cut(s)	2298
BstBAI	YACGTR	1 cut(s)	2387
BstC8I	GCNNGC	5 cut(s)	20, 90, 191, 2045, 2157
BstDEI	CTNAG	9 cut(s)	299, 399, 438, 471, 576, 669, 692, 824, 1976
BstDSI	CCRYGG	1 cut(s)	244
BstEII	GGTNACC	1 cut(s)	1286
BstENI	CCTNNNNNAGG	1 cut(s)	2017
BstFNI	CGCG	3 cut(s)	189, 246, 377
BstH2I	RGCGCY	1 cut(s)	252
BstHHI	GCGC	6 cut(s)	152, 189, 251, 377, 1098, 2314
BstKTI	GATC	8 cut(s)	477, 625, 862, 904, 1246, 1399, 2269, 2395
BstMAI	GTCTC	3 cut(s)	572, 732, 1290
BstMBI	GATC	8 cut(s)	474, 622, 859, 901, 1243, 1396, 2266, 2392
BstMWI	GCNNNNNNNGC	8 cut(s)	94, 190, 304, 404, 1847, 2126, 2156, 2320
BstNI	CCWGG	3 cut(s)	295, 1556, 2430
BstNSI	RCATGY	1 cut(s)	2029
BstPI	GGTNACC	1 cut(s)	1286
BstSCI	CCNGG	8 cut(s)	157, 237, 293, 1554, 1879, 2150, 2428, 2440
BstSFI	CTRYAG	3 cut(s)	660, 1002, 1665
BstSLI	GKGCMC	2 cut(s)	195, 290
BstUI	CGCG	3 cut(s)	189, 246, 377
BstV1I	GCAGC	7 cut(s)	196, 213, 754, 757, 1465, 2296, 2461
BstV2I	GAAGAC	4 cut(s)	375, 738, 1056, 1491
BstX2I	RGATCY	2 cut(s)	474, 1396
BstXI	CCANNNNNNTGG	1 cut(s)	1351
BstYI	RGATCY	2 cut(s)	474, 1396
BsuI	GTATCC	1 cut(s)	1571
BsuRI	GGCC	7 cut(s)	293, 968, 1554, 1751, 2155, 2323, 2440
BtgI	CCRYGG	1 cut(s)	244
BtgZI	GCGATG	1 cut(s)	217
BtrI	CACGTC	2 cut(s)	640, 1428
BtsI	GCAGTG	2 cut(s)	1181, 1522
BtsIMutI	CAGTG	6 cut(s)	1181, 1263, 1351, 1356, 1522, 2103
Cac8I	GCNNGC	5 cut(s)	20, 90, 191, 2045, 2157
CciI	TCATGA	1 cut(s)	349
CfoI	GCGC	6 cut(s)	152, 189, 251, 377, 1098, 2314
Cfr13I	GGNCC	4 cut(s)	241, 1607, 2426, 2439
Cfr42I	CCGCGG	1 cut(s)	247
CpoI	CGGWCCG	1 cut(s)	241
CseI	GACGC	1 cut(s)	205
CsiI	ACCWGGT	1 cut(s)	2428
CspCI	CAANNNNNGTGG	2 cut(s)	2148, 2183
CspI	CGGWCCG	1 cut(s)	241
CviAII	CATG	7 cut(s)	59, 81, 350, 863, 1319, 2026, 2122
DdeI	CTNAG	9 cut(s)	299, 399, 438, 471, 576, 669, 692, 824, 1976
DinI	GGCGCC	1 cut(s)	250
DpnI	GATC	8 cut(s)	476, 624, 861, 903, 1245, 1398, 2268, 2394
DpnII	GATC	8 cut(s)	474, 622, 859, 901, 1243, 1396, 2266, 2392
DraI	TTTAAA	1 cut(s)	1467
EaeI	YGGCCR	1 cut(s)	291
Eam1104I	CTCTTC	3 cut(s)	873, 2066, 2103
EarI	CTCTTC	3 cut(s)	873, 2066, 2103
EciI	GGCGGA	1 cut(s)	964
Ecl136II	GAGCTC	1 cut(s)	1139
Eco130I	CCWWGG	4 cut(s)	805, 1291, 1988, 2478
Eco147I	AGGCCT	2 cut(s)	968, 2323
Eco24I	GRGCYC	1 cut(s)	1141
Eco32I	GATATC	1 cut(s)	1651
Eco47I	GGWCC	3 cut(s)	241, 1607, 2426
Eco53kI	GAGCTC	1 cut(s)	1139
Eco57I	CTGAAG	4 cut(s)	463, 1380, 1680, 2532
Eco88I	CYCGRG	2 cut(s)	72, 281
Eco91I	GGTNACC	1 cut(s)	1286
EcoICRI	GAGCTC	1 cut(s)	1139
EcoNI	CCTNNNNNAGG	1 cut(s)	2017
EcoO65I	GGTNACC	1 cut(s)	1286
EcoRII	CCWGG	3 cut(s)	293, 1554, 2428
EcoRV	GATATC	1 cut(s)	1651
EcoT14I	CCWWGG	4 cut(s)	805, 1291, 1988, 2478
EcoT38I	GRGCYC	1 cut(s)	1141
EgeI	GGCGCC	1 cut(s)	250
EheI	GGCGCC	1 cut(s)	250
ErhI	CCWWGG	4 cut(s)	805, 1291, 1988, 2478
Esp3I	CGTCTC	1 cut(s)	1290
FaeI	CATG	7 cut(s)	62, 84, 353, 866, 1322, 2029, 2125
FaqI	GGGAC	3 cut(s)	212, 964, 1856
FatI	CATG	7 cut(s)	58, 80, 349, 862, 1318, 2025, 2121
FblI	GTMKAC	2 cut(s)	219, 504
FriOI	GRGCYC	1 cut(s)	1141
FspBI	CTAG	4 cut(s)	47, 224, 558, 1124
GlaI	GCGC	6 cut(s)	151, 188, 250, 376, 1097, 2313
GsaI	CCCAGC	2 cut(s)	97, 2534
HaeII	RGCGCY	1 cut(s)	252
HaeIII	GGCC	7 cut(s)	293, 968, 1554, 1751, 2155, 2323, 2440
HapII	CCGG	6 cut(s)	159, 239, 1880, 2152, 2442, 2505
HgaI	GACGC	1 cut(s)	205
HhaI	GCGC	6 cut(s)	152, 189, 251, 377, 1098, 2314
Hin1I	GRCGYC	2 cut(s)	216, 249
Hin1II	CATG	7 cut(s)	62, 84, 353, 866, 1322, 2029, 2125
Hin6I	GCGC	6 cut(s)	150, 187, 249, 375, 1096, 2312
HinP1I	GCGC	6 cut(s)	150, 187, 249, 375, 1096, 2312
HincII	GTYRAC	2 cut(s)	220, 2084
HindII	GTYRAC	2 cut(s)	220, 2084
HindIII	AAGCTT	5 cut(s)	86, 826, 1118, 1839, 2210
HinfI	GANTC	6 cut(s)	1259, 1541, 1568, 1573, 1889, 1916
HpaII	CCGG	6 cut(s)	159, 239, 1880, 2152, 2442, 2505
HphI	GGTGA	9 cut(s)	22, 265, 436, 1289, 1298, 1313, 1817, 2495, 2525
Hpy166II	GTNNAC	6 cut(s)	220, 288, 505, 1425, 2084, 2426
Hpy188III	TCNNGA	6 cut(s)	72, 350, 1142, 1616, 1646, 2550
Hpy8I	GTNNAC	6 cut(s)	220, 288, 505, 1425, 2084, 2426
Hpy99I	CGWCG	3 cut(s)	200, 221, 397
HpyCH4III	ACNGT	5 cut(s)	26, 719, 1301, 1422, 2081
HpyCH4IV	ACGT	5 cut(s)	639, 1082, 1427, 1462, 2386
HpyF10VI	GCNNNNNNNGC	8 cut(s)	94, 190, 304, 404, 1847, 2126, 2156, 2320
HpyF3I	CTNAG	9 cut(s)	299, 399, 438, 471, 576, 669, 692, 824, 1976
HpySE526I	ACGT	5 cut(s)	639, 1082, 1427, 1462, 2386
Hsp92I	GRCGYC	2 cut(s)	216, 249
Hsp92II	CATG	7 cut(s)	62, 84, 353, 866, 1322, 2029, 2125
HspAI	GCGC	6 cut(s)	150, 187, 249, 375, 1096, 2312
KasI	GGCGCC	1 cut(s)	248
KspI	CCGCGG	1 cut(s)	247
Kzo9I	GATC	8 cut(s)	474, 622, 859, 901, 1243, 1396, 2266, 2392
LmnI	GCTCC	7 cut(s)	74, 454, 520, 613, 1075, 1093, 1882
Lsp1109I	GCAGC	7 cut(s)	196, 213, 754, 757, 1465, 2296, 2461
MabI	ACCWGGT	1 cut(s)	2428
MaeI	CTAG	4 cut(s)	47, 224, 558, 1124
MaeII	ACGT	5 cut(s)	639, 1082, 1427, 1462, 2386
MaeIII	GTNAC	5 cut(s)	757, 1286, 2015, 2098, 2513
MalI	GATC	8 cut(s)	476, 624, 861, 903, 1245, 1398, 2268, 2394
MbiI	CCGCTC	1 cut(s)	69
MboI	GATC	8 cut(s)	474, 622, 859, 901, 1243, 1396, 2266, 2392
MflI	RGATCY	2 cut(s)	474, 1396
MhlI	GDGCHC	3 cut(s)	195, 290, 1141
Mly113I	GGCGCC	1 cut(s)	249
MmeI	TCCRAC	1 cut(s)	146
MroXI	GAANNNNTTC	1 cut(s)	2545
MseI	TTAA	8 cut(s)	597, 654, 869, 885, 1230, 1466, 2091, 2115
MslI	CAYNNNNRTG	1 cut(s)	720
MspA1I	CMGCKG	2 cut(s)	246, 1456
MspI	CCGG	6 cut(s)	159, 239, 1880, 2152, 2442, 2505
MspR9I	CCNGG	8 cut(s)	159, 239, 295, 1556, 1881, 2152, 2430, 2442
MvaI	CCWGG	3 cut(s)	295, 1556, 2430
MvnI	CGCG	3 cut(s)	189, 246, 377
MwoI	GCNNNNNNNGC	8 cut(s)	94, 190, 304, 404, 1847, 2126, 2156, 2320
NarI	GGCGCC	1 cut(s)	249
NciI	CCSGG	5 cut(s)	159, 239, 1881, 2152, 2442
NdeII	GATC	8 cut(s)	474, 622, 859, 901, 1243, 1396, 2266, 2392
NlaIII	CATG	7 cut(s)	62, 84, 353, 866, 1322, 2029, 2125
NlaIV	GGNNCC	5 cut(s)	250, 450, 609, 2078, 2503
NmeAIII	GCCGAG	1 cut(s)	1972
NmuCI	GTSAC	4 cut(s)	757, 1286, 2098, 2513
NspI	RCATGY	1 cut(s)	2029
PagI	TCATGA	1 cut(s)	349
PceI	AGGCCT	2 cut(s)	968, 2323
PciI	ACATGT	1 cut(s)	2025
PcsI	WCGNNNNNNNCGW	6 cut(s)	138, 204, 213, 411, 2191, 2392
PdmI	GAANNNNTTC	1 cut(s)	2545
PfeI	GAWTC	6 cut(s)	1259, 1541, 1568, 1573, 1889, 1916
PflFI	GACNNNGTC	1 cut(s)	224
PluTI	GGCGCC	1 cut(s)	252
Ppu21I	YACGTR	1 cut(s)	2387
PscI	ACATGT	1 cut(s)	2025
PshBI	ATTAAT	1 cut(s)	2115
PsiI	TTATAA	1 cut(s)	495
Psp124BI	GAGCTC	1 cut(s)	1141
Psp1406I	AACGTT	1 cut(s)	1462
Psp6I	CCWGG	3 cut(s)	293, 1554, 2428
PspEI	GGTNACC	1 cut(s)	1286
PspFI	CCCAGC	2 cut(s)	93, 2530
PspGI	CCWGG	3 cut(s)	293, 1554, 2428
PspN4I	GGNNCC	5 cut(s)	250, 450, 609, 2078, 2503
PspPI	GGNCC	4 cut(s)	241, 1607, 2426, 2439
PsrI	GAACNNNNNNTAC	2 cut(s)	451, 483
PsuI	RGATCY	2 cut(s)	474, 1396
PsyI	GACNNNGTC	1 cut(s)	224
RseI	CAYNNNNRTG	1 cut(s)	720
Rsr2I	CGGWCCG	1 cut(s)	241
RsrII	CGGWCCG	1 cut(s)	241
SacI	GAGCTC	1 cut(s)	1141
SacII	CCGCGG	1 cut(s)	247
SalI	GTCGAC	1 cut(s)	218
SaqAI	TTAA	8 cut(s)	597, 654, 869, 885, 1230, 1466, 2091, 2115
Sau3AI	GATC	8 cut(s)	474, 622, 859, 901, 1243, 1396, 2266, 2392
Sau96I	GGNCC	4 cut(s)	241, 1607, 2426, 2439
ScaI	AGTACT	1 cut(s)	38
ScrFI	CCNGG	8 cut(s)	159, 239, 295, 1556, 1881, 2152, 2430, 2442
SduI	GDGCHC	3 cut(s)	195, 290, 1141
SexAI	ACCWGGT	1 cut(s)	2428
SfcI	CTRYAG	3 cut(s)	660, 1002, 1665
SfoI	GGCGCC	1 cut(s)	250
Sfr303I	CCGCGG	1 cut(s)	247
SgrBI	CCGCGG	1 cut(s)	247
SinI	GGWCC	3 cut(s)	241, 1607, 2426
SmiMI	CAYNNNNRTG	1 cut(s)	720
SmlI	CTYRAG	4 cut(s)	854, 1140, 1614, 2213
SmoI	CTYRAG	4 cut(s)	854, 1140, 1614, 2213
SseBI	AGGCCT	2 cut(s)	968, 2323
SsiI	CCGC	9 cut(s)	67, 153, 162, 244, 246, 422, 555, 949, 1456
SspDI	GGCGCC	1 cut(s)	248
SspMI	CTAG	4 cut(s)	47, 224, 558, 1124
SstI	GAGCTC	1 cut(s)	1141
StuI	AGGCCT	2 cut(s)	968, 2323
StyD4I	CCNGG	8 cut(s)	157, 237, 293, 1554, 1879, 2150, 2428, 2440
StyI	CCWWGG	4 cut(s)	805, 1291, 1988, 2478
TaaI	ACNGT	5 cut(s)	26, 719, 1301, 1422, 2081
TaiI	ACGT	5 cut(s)	642, 1085, 1430, 1465, 2389
TaqI	TCGA	8 cut(s)	99, 141, 219, 336, 1404, 1571, 2185, 2395
TatI	WGTACW	5 cut(s)	36, 600, 688, 1422, 2298
TauI	GCSGC	3 cut(s)	155, 249, 557
TfiI	GAWTC	6 cut(s)	1259, 1541, 1568, 1573, 1889, 1916
Tru1I	TTAA	8 cut(s)	597, 654, 869, 885, 1230, 1466, 2091, 2115
Tru9I	TTAA	8 cut(s)	597, 654, 869, 885, 1230, 1466, 2091, 2115
TscAI	CASTG	6 cut(s)	1188, 1270, 1351, 1363, 1522, 2103
TseFI	GTSAC	4 cut(s)	757, 1286, 2098, 2513
TseI	GCWGC	7 cut(s)	184, 201, 767, 770, 1453, 2309, 2474
Tsp45I	GTSAC	4 cut(s)	757, 1286, 2098, 2513
TspGWI	ACGGA	3 cut(s)	750, 966, 1168
TspRI	CASTG	6 cut(s)	1188, 1270, 1351, 1363, 1522, 2103
Tth111I	GACNNNGTC	1 cut(s)	224
VneI	GTGCAC	1 cut(s)	286
VpaK11BI	GGWCC	3 cut(s)	241, 1607, 2426
VspI	ATTAAT	1 cut(s)	2115
XagI	CCTNNNNNAGG	1 cut(s)	2017
XapI	RAATTY	1 cut(s)	1730
XceI	RCATGY	1 cut(s)	2029
XmiI	GTMKAC	2 cut(s)	219, 504
XmnI	GAANNNNTTC	1 cut(s)	2545
XspI	CTAG	4 cut(s)	47, 224, 558, 1124
ZrmI	AGTACT	1 cut(s)	38

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.