FvH4_2g10530

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013608
Physical Location & Seq
Forward (+)
9332787 .. 9333415
629 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g10530.t1

Sequence Viewer

Length: 597 bp
ATGAATCCAGTGCTTTCTGACTTCATTTCTTTGATCACCTTCATGGTGCTCTGTGTCATAGTTGGCATACTTGAAGAGAAAATCAAAGTCGCCAGACCTGGATTGGCAAAACCCATAGATGTCCTCGTGAATACACTCATGATAGTAATAATCTATCTTGTCCGCAACATTGTGCTTGGTACCCTTTCCATTGTTGATCCCACTGTCGATTATTGGATCGTAACAGGTCTCAGCTATGGATTTTATATCCCAATGTTACCCTGCGTATATGACATACTAAGCACCCTTGGCTCAACAAGGAGGATGGTTAAACTGATGCATGATGCTACTTTTCTTCTGGTTGCAATCGGTGCCAAGAGCCCTCAAGGCTATGCGATTTTCAGTTATCAAATGGACCATGAAAAGGGGTTTTGTAAAGGCGAGTGTGCTATTTGCTTGATGGAGTACGAGACCAAAGACAAGTGTGCGTTGTTAGATAAATGTGGACACACGTTTCACCACAAATGCCTGTATGAGAATTTGGCTGTGTCTACCAGTTGTCCGCTTTGCCGTGAATGTATCAAAGATAGTGCACCGGAAAACAAAGAACTCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

199

Amino Acids

22.11

Weight (kDa)

6.19

Isoelectric Point (pI)

32.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 140 - 184 4e-06 RING-H2 zinc finger domain
zf-RING_2 PF13639 141 - 184 2.9e-12 Ring finger domain
zf-RING_11 PF17123 141 - 170 6.3e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 141 - 183 3e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 141 - 184 2.9e-06 zinc-RING finger domain
zf-C3HC4 PF00097 142 - 183 1.5e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 142 - 181 2.9e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 179
AccB1I GGYRCC 2 cut(s) 179, 350
AccI GTMKAC 1 cut(s) 530
AciI CCGC 2 cut(s) 163, 542
AclWI GGATC 2 cut(s) 191, 224
AcsI RAATTY 1 cut(s) 517
AfaI GTAC 2 cut(s) 181, 446
AfiI CCNNNNNNNGG 1 cut(s) 403
AflIII ACRYGT 1 cut(s) 489
AgsI TTSAA 1 cut(s) 74
AjnI CCWGG 1 cut(s) 97
AluBI AGCT 1 cut(s) 234
AluI AGCT 1 cut(s) 234
Alw21I GWGCWC 2 cut(s) 51, 574
Alw26I GTCTC 2 cut(s) 233, 443
Alw44I GTGCAC 1 cut(s) 570
AlwI GGATC 2 cut(s) 191, 224
ApaLI GTGCAC 1 cut(s) 570
ApoI RAATTY 1 cut(s) 517
Asp718I GGTACC 1 cut(s) 179
AspS9I GGNCC 1 cut(s) 394
AsuHPI GGTGA 2 cut(s) 28, 488
AvaII GGWCC 1 cut(s) 394
BaeGI GKGCMC 1 cut(s) 574
BanI GGYRCC 2 cut(s) 179, 350
BanII GRGCYC 1 cut(s) 362
BauI CACGAG 1 cut(s) 125
Bbv12I GWGCWC 2 cut(s) 51, 574
BccI CCATC 2 cut(s) 298, 433
BceAI ACGGC 1 cut(s) 534
BciT130I CCWGG 1 cut(s) 99
BclI TGATCA 1 cut(s) 33
BcoDI GTCTC 2 cut(s) 233, 443
BglI GCCNNNNNGGC 1 cut(s) 366
Bme1390I CCNGG 1 cut(s) 99
Bme18I GGWCC 1 cut(s) 394
BmgT120I GGNCC 1 cut(s) 394
BmiI GGNNCC 2 cut(s) 181, 352
BmrFI CCNGG 1 cut(s) 99
BmsI GCATC 2 cut(s) 306, 313
BpuEI CTTGAG 1 cut(s) 348
BsaI GGTCTC 2 cut(s) 233, 443
BsaJI CCNNGG 1 cut(s) 286
BsaWI WCCGGW 1 cut(s) 574
Bsc4I CCNNNNNNNGG 1 cut(s) 403
Bse1I ACTGG 2 cut(s) 8, 534
BseBI CCWGG 1 cut(s) 99
BseDI CCNNGG 1 cut(s) 286
BseGI GGATG 1 cut(s) 309
BseLI CCNNNNNNNGG 1 cut(s) 403
BseMII CTCAG 1 cut(s) 244
BseNI ACTGG 2 cut(s) 8, 534
BseSI GKGCMC 1 cut(s) 574
BshNI GGYRCC 2 cut(s) 179, 350
BsiHKAI GWGCWC 2 cut(s) 51, 574
BsiSI CCGG 1 cut(s) 575
BslI CCNNNNNNNGG 1 cut(s) 403
BsmAI GTCTC 2 cut(s) 233, 443
Bso31I GGTCTC 2 cut(s) 233, 443
Bsp1286I GDGCHC 3 cut(s) 51, 362, 574
Bsp143I GATC 3 cut(s) 33, 196, 216
BspACI CCGC 2 cut(s) 163, 542
BspCNI CTCAG 1 cut(s) 243
BspHI TCATGA 1 cut(s) 138
BspLI GGNNCC 2 cut(s) 181, 352
BspPI GGATC 2 cut(s) 191, 224
BspT107I GGYRCC 2 cut(s) 179, 350
BspTNI GGTCTC 2 cut(s) 233, 443
BsrI ACTGG 2 cut(s) 8, 534
BssECI CCNNGG 1 cut(s) 286
BssMI GATC 3 cut(s) 33, 196, 216
BssSI CACGAG 1 cut(s) 125
BssT1I CCWWGG 1 cut(s) 286
Bst2BI CACGAG 1 cut(s) 125
Bst2UI CCWGG 1 cut(s) 99
Bst4CI ACNGT 1 cut(s) 205
Bst6I CTCTTC 1 cut(s) 69
BstAPI GCANNNNNTGC 1 cut(s) 350
BstDEI CTNAG 3 cut(s) 230, 278, 594
BstF5I GGATG 1 cut(s) 309
BstKTI GATC 3 cut(s) 36, 199, 219
BstMAI GTCTC 2 cut(s) 233, 443
BstMBI GATC 3 cut(s) 33, 196, 216
BstMWI GCNNNNNNNGC 3 cut(s) 288, 350, 366
BstNI CCWGG 1 cut(s) 99
BstSCI CCNGG 1 cut(s) 97
BstSLI GKGCMC 1 cut(s) 574
BtsCI GGATG 1 cut(s) 309
BtsIMutI CAGTG 2 cut(s) 15, 201
CciI TCATGA 1 cut(s) 138
Cfr13I GGNCC 1 cut(s) 394
Csp6I GTAC 2 cut(s) 180, 445
CviAII CATG 4 cut(s) 43, 139, 320, 398
CviJI RGCY 5 cut(s) 234, 291, 360, 369, 524
CviKI_1 RGCY 5 cut(s) 234, 291, 360, 369, 524
CviQI GTAC 2 cut(s) 180, 445
DdeI CTNAG 3 cut(s) 230, 278, 594
DpnI GATC 3 cut(s) 35, 198, 218
DpnII GATC 3 cut(s) 33, 196, 216
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 286
Eco24I GRGCYC 1 cut(s) 362
Eco31I GGTCTC 2 cut(s) 233, 443
Eco47I GGWCC 1 cut(s) 394
EcoRII CCWGG 1 cut(s) 97
EcoT14I CCWWGG 1 cut(s) 286
EcoT22I ATGCAT 1 cut(s) 321
EcoT38I GRGCYC 1 cut(s) 362
ErhI CCWWGG 1 cut(s) 286
FaeI CATG 4 cut(s) 46, 142, 323, 401
FatI CATG 4 cut(s) 42, 138, 319, 397
FbaI TGATCA 1 cut(s) 33
FblI GTMKAC 1 cut(s) 530
FokI GGATG 1 cut(s) 316
FriOI GRGCYC 1 cut(s) 362
HapII CCGG 1 cut(s) 575
Hin1II CATG 4 cut(s) 46, 142, 323, 401
HinfI GANTC 1 cut(s) 4
HpaII CCGG 1 cut(s) 575
HphI GGTGA 2 cut(s) 28, 488
Hpy166II GTNNAC 3 cut(s) 485, 531, 572
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 2 cut(s) 127, 139
Hpy8I GTNNAC 3 cut(s) 485, 531, 572
HpyAV CCTTC 1 cut(s) 49
HpyCH4III ACNGT 1 cut(s) 205
HpyCH4IV ACGT 1 cut(s) 491
HpyCH4V TGCA 3 cut(s) 319, 344, 572
HpyF10VI GCNNNNNNNGC 3 cut(s) 288, 350, 366
HpyF3I CTNAG 3 cut(s) 230, 278, 594
HpySE526I ACGT 1 cut(s) 491
Hsp92II CATG 4 cut(s) 46, 142, 323, 401
KpnI GGTACC 1 cut(s) 183
Ksp22I TGATCA 1 cut(s) 33
Kzo9I GATC 3 cut(s) 33, 196, 216
LweI GCATC 2 cut(s) 306, 313
MaeII ACGT 1 cut(s) 491
MaeIII GTNAC 2 cut(s) 220, 255
MalI GATC 3 cut(s) 35, 198, 218
MboI GATC 3 cut(s) 33, 196, 216
MboII GAAGA 2 cut(s) 86, 326
MhlI GDGCHC 3 cut(s) 51, 362, 574
MluCI AATT 1 cut(s) 517
MnlI CCTC 3 cut(s) 134, 294, 372
Mph1103I ATGCAT 1 cut(s) 321
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 41
MspI CCGG 1 cut(s) 575
MspR9I CCNGG 1 cut(s) 99
MvaI CCWGG 1 cut(s) 99
MwoI GCNNNNNNNGC 3 cut(s) 288, 350, 366
NdeII GATC 3 cut(s) 33, 196, 216
NlaIII CATG 4 cut(s) 46, 142, 323, 401
NlaIV GGNNCC 2 cut(s) 181, 352
NsiI ATGCAT 1 cut(s) 321
PagI TCATGA 1 cut(s) 138
PfeI GAWTC 1 cut(s) 4
Psp6I CCWGG 1 cut(s) 97
PspGI CCWGG 1 cut(s) 97
PspN4I GGNNCC 2 cut(s) 181, 352
PspPI GGNCC 1 cut(s) 394
RsaI GTAC 2 cut(s) 181, 446
RsaNI GTAC 2 cut(s) 180, 445
RseI CAYNNNNRTG 1 cut(s) 41
SaqAI TTAA 1 cut(s) 309
Sau3AI GATC 3 cut(s) 33, 196, 216
Sau96I GGNCC 1 cut(s) 394
ScrFI CCNGG 1 cut(s) 99
SduI GDGCHC 3 cut(s) 51, 362, 574
SetI ASST 5 cut(s) 41, 100, 229, 236, 494
SfaNI GCATC 2 cut(s) 306, 313
SinI GGWCC 1 cut(s) 394
SmiMI CAYNNNNRTG 1 cut(s) 41
SmlI CTYRAG 1 cut(s) 363
SmoI CTYRAG 1 cut(s) 363
Sse9I AATT 1 cut(s) 517
SsiI CCGC 2 cut(s) 163, 542
StyD4I CCNGG 1 cut(s) 97
StyI CCWWGG 1 cut(s) 286
TaaI ACNGT 1 cut(s) 205
TaiI ACGT 1 cut(s) 494
TaqI TCGA 1 cut(s) 207
TasI AATT 1 cut(s) 517
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 2 cut(s) 15, 208
TspDTI ATGAA 4 cut(s) 13, 17, 31, 414
TspRI CASTG 2 cut(s) 15, 208
VneI GTGCAC 1 cut(s) 570
VpaK11BI GGWCC 1 cut(s) 394
XapI RAATTY 1 cut(s) 517
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 530
Zsp2I ATGCAT 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.