RLG00000014128

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013608
Physical Location & Seq
Forward (+)
47782841 .. 47783434
594 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014128

Sequence Viewer

Length: 594 bp
ATGGTTTCTGCACTAGTTATATTGATTCCCACCGTCATAGTTCTCTGTGTCATAGTTGGTGTACTCGAAGAGAAAATCAGAGTCACCAGACCTGGATTTGCCCCCGCCATAGATATCACCGTGAGTATACTCATGATACTAGTTATTTTTCTTTTCTGCACCATTGTGCTTGGTACTATTTCCATTGTTGAACCCTCCCTCGATTACAGGATCGTAATAGGCCTCGGCTATGGATTTTATATCCTAATGTTACCCTATGTGTATGACATAATAATCACCATTGGCTCATGTTGTTCAAGAACGATGATTAAACTGATGCACAACGCGACTTTTCTTATGGTGACAATCAGTCCCAAGAGCCCTGAAGGCTATGCAATTTTCAGTTATCAAATGGACCAAGAAAAAGGGTTTTGCAGAGGCGAGTGTGCTATTTGCTTGATGGAGTACGAGACAAAAGACAAATGTGCATTGTTAGATAAATGTGGACATACGTTTCACCACAAATGCCTATATGAGAATTTGGCTATGTCTACCCGATGTCCTCTTTGTCATCAAATTATCAAAGATGGTAATGAACTCCATGTAGTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

198

Amino Acids

22.07

Weight (kDa)

6.06

Isoelectric Point (pI)

39.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 141 - 183 1.4e-11 Ring finger domain
zf-RING_11 PF17123 141 - 170 6.3e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 141 - 183 1.8e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 141 - 185 9.1e-06 zinc-RING finger domain
zf-RING_UBOX PF13445 142 - 181 5.1e-07 RING-type zinc-finger
zf-C3HC4 PF00097 142 - 183 8.6e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 127, 530
AccII CGCG 1 cut(s) 326
AciI CCGC 1 cut(s) 105
AclWI GGATC 1 cut(s) 218
AcsI RAATTY 1 cut(s) 517
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 3 cut(s) 63, 175, 446
AgsI TTSAA 3 cut(s) 191, 297, 590
AhdI GACNNNNNGTC 1 cut(s) 348
AhlI ACTAGT 2 cut(s) 13, 139
AjnI CCWGG 1 cut(s) 91
AleI CACNNNNGTG 1 cut(s) 164
Alw26I GTCTC 1 cut(s) 443
AlwI GGATC 1 cut(s) 218
AoxI GGCC 1 cut(s) 220
ApoI RAATTY 1 cut(s) 517
AspS9I GGNCC 1 cut(s) 394
AsuHPI GGTGA 5 cut(s) 76, 109, 268, 352, 488
AvaII GGWCC 1 cut(s) 394
BanII GRGCYC 1 cut(s) 362
BccI CCATC 2 cut(s) 433, 560
BciT130I CCWGG 1 cut(s) 93
BcoDI GTCTC 1 cut(s) 443
BcuI ACTAGT 2 cut(s) 13, 139
BfaI CTAG 2 cut(s) 14, 140
BglI GCCNNNNNGGC 1 cut(s) 366
Bme1390I CCNGG 1 cut(s) 93
Bme18I GGWCC 1 cut(s) 394
BmeRI GACNNNNNGTC 1 cut(s) 348
BmgT120I GGNCC 1 cut(s) 394
BmrFI CCNGG 1 cut(s) 93
BmsI GCATC 1 cut(s) 306
BsaJI CCNNGG 1 cut(s) 223
BseBI CCWGG 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 223
BsgI GTGCAG 1 cut(s) 142
Bsh1236I CGCG 1 cut(s) 326
BshFI GGCC 1 cut(s) 222
BslFI GGGAC 1 cut(s) 336
BsmAI GTCTC 1 cut(s) 443
BsmFI GGGAC 1 cut(s) 336
BsnI GGCC 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 362
Bsp143I GATC 1 cut(s) 210
BspACI CCGC 1 cut(s) 105
BspANI GGCC 1 cut(s) 222
BspFNI CGCG 1 cut(s) 326
BspHI TCATGA 1 cut(s) 132
BspPI GGATC 1 cut(s) 218
BssECI CCNNGG 1 cut(s) 223
BssMI GATC 1 cut(s) 210
BssNAI GTATAC 1 cut(s) 128
Bst1107I GTATAC 1 cut(s) 128
Bst2UI CCWGG 1 cut(s) 93
Bst4CI ACNGT 2 cut(s) 34, 121
Bst6I CTCTTC 1 cut(s) 63
BstFNI CGCG 1 cut(s) 326
BstKTI GATC 1 cut(s) 213
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 1 cut(s) 210
BstMWI GCNNNNNNNGC 1 cut(s) 366
BstNI CCWGG 1 cut(s) 93
BstSCI CCNGG 1 cut(s) 91
BstUI CGCG 1 cut(s) 326
BstZ17I GTATAC 1 cut(s) 128
BsuRI GGCC 1 cut(s) 222
CciI TCATGA 1 cut(s) 132
Cfr13I GGNCC 1 cut(s) 394
Csp6I GTAC 3 cut(s) 62, 174, 445
CviAII CATG 3 cut(s) 133, 288, 581
CviJI RGCY 6 cut(s) 222, 228, 285, 360, 369, 524
CviKI_1 RGCY 6 cut(s) 222, 228, 285, 360, 369, 524
CviQI GTAC 3 cut(s) 62, 174, 445
DpnI GATC 1 cut(s) 212
DpnII GATC 1 cut(s) 210
DriI GACNNNNNGTC 1 cut(s) 348
Eam1104I CTCTTC 1 cut(s) 63
Eam1105I GACNNNNNGTC 1 cut(s) 348
EarI CTCTTC 1 cut(s) 63
Eco147I AGGCCT 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 362
Eco32I GATATC 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 394
Eco57I CTGAAG 1 cut(s) 384
EcoRII CCWGG 1 cut(s) 91
EcoRV GATATC 1 cut(s) 115
EcoT38I GRGCYC 1 cut(s) 362
FaeI CATG 3 cut(s) 136, 291, 584
FaqI GGGAC 1 cut(s) 336
FatI CATG 3 cut(s) 132, 287, 580
FauI CCCGC 1 cut(s) 112
FblI GTMKAC 2 cut(s) 127, 530
FriOI GRGCYC 1 cut(s) 362
FspBI CTAG 2 cut(s) 14, 140
HaeIII GGCC 1 cut(s) 222
Hin1II CATG 3 cut(s) 136, 291, 584
HinfI GANTC 2 cut(s) 25, 81
HphI GGTGA 5 cut(s) 76, 109, 268, 352, 488
Hpy166II GTNNAC 4 cut(s) 62, 128, 485, 531
Hpy188I TCNGA 1 cut(s) 80
Hpy188III TCNNGA 2 cut(s) 133, 297
Hpy8I GTNNAC 4 cut(s) 62, 128, 485, 531
HpyAV CCTTC 1 cut(s) 359
HpyCH4III ACNGT 2 cut(s) 34, 121
HpyCH4IV ACGT 1 cut(s) 491
HpyCH4V TGCA 6 cut(s) 11, 159, 319, 374, 414, 467
HpyF10VI GCNNNNNNNGC 1 cut(s) 366
HpySE526I ACGT 1 cut(s) 491
Hsp92II CATG 3 cut(s) 136, 291, 584
Kzo9I GATC 1 cut(s) 210
LpnPI CCDG 5 cut(s) 78, 100, 105, 193, 375
LweI GCATC 1 cut(s) 306
MaeI CTAG 2 cut(s) 14, 140
MaeII ACGT 1 cut(s) 491
MaeIII GTNAC 3 cut(s) 82, 249, 340
MalI GATC 1 cut(s) 212
MboI GATC 1 cut(s) 210
MboII GAAGA 1 cut(s) 80
MhlI GDGCHC 1 cut(s) 362
MluCI AATT 3 cut(s) 375, 517, 555
MlyI GAGTC 1 cut(s) 90
MnlI CCTC 5 cut(s) 205, 209, 233, 410, 552
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 164
MspR9I CCNGG 1 cut(s) 93
MvaI CCWGG 1 cut(s) 93
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 1 cut(s) 366
NdeII GATC 1 cut(s) 210
NlaIII CATG 3 cut(s) 136, 291, 584
NmeAIII GCCGAG 1 cut(s) 204
NmuCI GTSAC 2 cut(s) 82, 340
OliI CACNNNNGTG 1 cut(s) 164
PagI TCATGA 1 cut(s) 132
PceI AGGCCT 1 cut(s) 222
PfeI GAWTC 1 cut(s) 25
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Psp6I CCWGG 1 cut(s) 91
PspGI CCWGG 1 cut(s) 91
PspPI GGNCC 1 cut(s) 394
RsaI GTAC 3 cut(s) 63, 175, 446
RsaNI GTAC 3 cut(s) 62, 174, 445
RseI CAYNNNNRTG 1 cut(s) 164
SaqAI TTAA 1 cut(s) 309
Sau3AI GATC 1 cut(s) 210
Sau96I GGNCC 1 cut(s) 394
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 1 cut(s) 93
SduI GDGCHC 1 cut(s) 362
SetI ASST 2 cut(s) 94, 494
SfaNI GCATC 1 cut(s) 306
SinI GGWCC 1 cut(s) 394
SmiMI CAYNNNNRTG 1 cut(s) 164
SpeI ACTAGT 2 cut(s) 13, 139
Sse9I AATT 3 cut(s) 375, 517, 555
SseBI AGGCCT 1 cut(s) 222
SsiI CCGC 1 cut(s) 105
SspMI CTAG 2 cut(s) 14, 140
StuI AGGCCT 1 cut(s) 222
StyD4I CCNGG 1 cut(s) 91
TaaI ACNGT 2 cut(s) 34, 121
TaiI ACGT 1 cut(s) 494
TaqI TCGA 2 cut(s) 66, 201
TasI AATT 3 cut(s) 375, 517, 555
TatI WGTACW 1 cut(s) 61
TfiI GAWTC 1 cut(s) 25
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TseFI GTSAC 2 cut(s) 82, 340
Tsp45I GTSAC 2 cut(s) 82, 340
TspDTI ATGAA 1 cut(s) 588
VpaK11BI GGWCC 1 cut(s) 394
XapI RAATTY 1 cut(s) 517
XmiI GTMKAC 2 cut(s) 127, 530
XspI CTAG 2 cut(s) 14, 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.