Rh6AG159300

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0013608
Physical Location & Seq
Forward (+)
24931039 .. 24931647
609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG159300.1

Sequence Viewer

Length: 609 bp
ATGAATCCTGTGATTTCTGCACTGATTTTATTGGTTCCCACCGGCGTAGTCCTCTGGGTCACAGTTGGCGTACTCGAAGAGAAAATCAGAGTCGCCAGACCTGGATTCGCCCCCGCCATAGATATCGCCGTGAGTATACTCACGATACTAGTTATTTTTCTTTTCCGCACCATTGCGCTTGGTACCATTTCCATTGTTGAACCCTCCCTCGATTACAGGATCGTAACAGGCCTCGGCTATGGATTTTATATCCCAATGTTACCCCGGGTATATGACATAATAACCACCGTTGGCTCATGTTGTTCAAGAACAATGGTTAAACTGATGCACAATGCTACTTTTCTTATGGTGACAATCGATGCCAAGAGCCCTGAAGGCTATGCGATTTTCAATTATCAAGTGGACCGAGAAAAGGGGTTTTGCAGAGGCGAGTGTGCCATTTGCTTGATGGAGTACGAGACAAGAGACAAGTGTGCATTGTTAGATAAATGTGGACATACGTTTCACCACAAATGTCTATATGAGAATTTGGCTATGTCTACCAGATGTCCGCTTTGTCGTCAAAGTATCAAAGATAGTAAGGAACTCCATGCAGCTGCCAGTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

202

Amino Acids

22.46

Weight (kDa)

8.02

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 141 - 187 4e-06 RING-H2 zinc finger domain
zf-RING_2 PF13639 144 - 187 1.4e-12 Ring finger domain
zf-RING_11 PF17123 144 - 173 9.2e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 144 - 186 1.7e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 144 - 188 5.4e-06 zinc-RING finger domain
zf-RING_UBOX PF13445 145 - 184 1.4e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 182
AccB1I GGYRCC 1 cut(s) 182
AccI GTMKAC 2 cut(s) 136, 539
AciI CCGC 3 cut(s) 114, 166, 551
AclWI GGATC 1 cut(s) 227
AcsI RAATTY 1 cut(s) 526
AcuI CTGAAG 1 cut(s) 393
AfaI GTAC 3 cut(s) 72, 184, 455
AfiI CCNNNNNNNGG 1 cut(s) 412
AgsI TTSAA 3 cut(s) 200, 306, 391
AhlI ACTAGT 1 cut(s) 148
AjnI CCWGG 1 cut(s) 100
AluBI AGCT 1 cut(s) 596
AluI AGCT 1 cut(s) 596
Alw26I GTCTC 2 cut(s) 452, 459
AlwI GGATC 1 cut(s) 227
Ama87I CYCGRG 1 cut(s) 264
AoxI GGCC 1 cut(s) 229
ApeKI GCWGC 2 cut(s) 593, 596
ApoI RAATTY 1 cut(s) 526
Asp718I GGTACC 1 cut(s) 182
AspLEI GCGC 1 cut(s) 178
AspS9I GGNCC 1 cut(s) 403
AsuC2I CCSGG 2 cut(s) 265, 266
AsuHPI GGTGA 2 cut(s) 361, 497
AvaI CYCGRG 1 cut(s) 264
AvaII GGWCC 1 cut(s) 403
BanI GGYRCC 1 cut(s) 182
BanII GRGCYC 1 cut(s) 371
BbvI GCAGC 2 cut(s) 583, 605
BccI CCATC 1 cut(s) 442
BceAI ACGGC 1 cut(s) 113
BciT130I CCWGG 1 cut(s) 102
BcnI CCSGG 2 cut(s) 265, 266
BcoDI GTCTC 2 cut(s) 452, 459
BcuI ACTAGT 1 cut(s) 148
BfaI CTAG 1 cut(s) 149
BglI GCCNNNNNGGC 1 cut(s) 375
BisI GCNGC 2 cut(s) 594, 597
BlsI GCNGC 2 cut(s) 595, 598
Bme1390I CCNGG 3 cut(s) 102, 265, 266
Bme18I GGWCC 1 cut(s) 403
BmeT110I CYCGRG 1 cut(s) 264
BmgT120I GGNCC 1 cut(s) 403
BmiI GGNNCC 2 cut(s) 36, 184
BmrFI CCNGG 3 cut(s) 102, 265, 266
BmsI GCATC 2 cut(s) 315, 349
BpuMI CCSGG 2 cut(s) 265, 266
Bsa29I ATCGAT 1 cut(s) 357
BsaJI CCNNGG 3 cut(s) 232, 263, 264
Bsc4I CCNNNNNNNGG 1 cut(s) 412
Bse118I RCCGGY 1 cut(s) 41
Bse1I ACTGG 1 cut(s) 600
Bse3DI GCAATG 1 cut(s) 171
BseBI CCWGG 1 cut(s) 102
BseCI ATCGAT 1 cut(s) 357
BseDI CCNNGG 3 cut(s) 232, 263, 264
BseLI CCNNNNNNNGG 1 cut(s) 412
BseMI GCAATG 1 cut(s) 171
BseNI ACTGG 1 cut(s) 600
BseXI GCAGC 2 cut(s) 583, 605
BshFI GGCC 1 cut(s) 231
BshNI GGYRCC 1 cut(s) 182
BshVI ATCGAT 1 cut(s) 357
BsiHKCI CYCGRG 1 cut(s) 264
BsiSI CCGG 2 cut(s) 42, 265
BslI CCNNNNNNNGG 1 cut(s) 412
BsmAI GTCTC 2 cut(s) 452, 459
BsnI GGCC 1 cut(s) 231
BsoBI CYCGRG 1 cut(s) 264
Bsp1286I GDGCHC 1 cut(s) 371
Bsp143I GATC 1 cut(s) 219
BspACI CCGC 3 cut(s) 114, 166, 551
BspANI GGCC 1 cut(s) 231
BspDI ATCGAT 1 cut(s) 357
BspLI GGNNCC 2 cut(s) 36, 184
BspPI GGATC 1 cut(s) 227
BspT107I GGYRCC 1 cut(s) 182
BsrDI GCAATG 1 cut(s) 171
BsrFI RCCGGY 1 cut(s) 41
BsrI ACTGG 1 cut(s) 600
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 3 cut(s) 232, 263, 264
BssMI GATC 1 cut(s) 219
BssNAI GTATAC 1 cut(s) 137
Bst1107I GTATAC 1 cut(s) 137
Bst2UI CCWGG 1 cut(s) 102
Bst4CI ACNGT 2 cut(s) 64, 289
Bst6I CTCTTC 1 cut(s) 72
BstHHI GCGC 1 cut(s) 178
BstKTI GATC 1 cut(s) 222
BstMAI GTCTC 2 cut(s) 452, 459
BstMBI GATC 1 cut(s) 219
BstMWI GCNNNNNNNGC 1 cut(s) 375
BstNI CCWGG 1 cut(s) 102
BstSCI CCNGG 3 cut(s) 100, 263, 264
BstV1I GCAGC 2 cut(s) 583, 605
BstZ17I GTATAC 1 cut(s) 137
Bsu15I ATCGAT 1 cut(s) 357
BsuRI GGCC 1 cut(s) 231
BsuTUI ATCGAT 1 cut(s) 357
BtsIMutI CAGTG 1 cut(s) 20
CfoI GCGC 1 cut(s) 178
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 1 cut(s) 403
Cfr9I CCCGGG 1 cut(s) 264
ClaI ATCGAT 1 cut(s) 357
Csp6I GTAC 3 cut(s) 71, 183, 454
CviAII CATG 2 cut(s) 297, 590
CviJI RGCY 7 cut(s) 231, 237, 294, 369, 378, 533, 596
CviKI_1 RGCY 7 cut(s) 231, 237, 294, 369, 378, 533, 596
CviQI GTAC 3 cut(s) 71, 183, 454
DpnI GATC 1 cut(s) 221
DpnII GATC 1 cut(s) 219
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
Eco147I AGGCCT 1 cut(s) 231
Eco24I GRGCYC 1 cut(s) 371
Eco32I GATATC 1 cut(s) 124
Eco47I GGWCC 1 cut(s) 403
Eco57I CTGAAG 1 cut(s) 393
Eco88I CYCGRG 1 cut(s) 264
EcoRII CCWGG 1 cut(s) 100
EcoRV GATATC 1 cut(s) 124
EcoT38I GRGCYC 1 cut(s) 371
FaeI CATG 2 cut(s) 300, 593
FatI CATG 2 cut(s) 296, 589
FauI CCCGC 1 cut(s) 121
FblI GTMKAC 2 cut(s) 136, 539
Fnu4HI GCNGC 2 cut(s) 594, 597
FriOI GRGCYC 1 cut(s) 371
Fsp4HI GCNGC 2 cut(s) 594, 597
FspBI CTAG 1 cut(s) 149
GlaI GCGC 1 cut(s) 177
GluI GCNGC 2 cut(s) 594, 597
HaeIII GGCC 1 cut(s) 231
HapII CCGG 2 cut(s) 42, 265
HhaI GCGC 1 cut(s) 178
Hin1II CATG 2 cut(s) 300, 593
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HinfI GANTC 3 cut(s) 4, 90, 105
HpaII CCGG 2 cut(s) 42, 265
HphI GGTGA 2 cut(s) 361, 497
Hpy166II GTNNAC 4 cut(s) 137, 403, 494, 540
Hpy188I TCNGA 1 cut(s) 89
Hpy188III TCNNGA 2 cut(s) 142, 306
Hpy8I GTNNAC 4 cut(s) 137, 403, 494, 540
HpyAV CCTTC 1 cut(s) 368
HpyCH4III ACNGT 2 cut(s) 64, 289
HpyCH4IV ACGT 1 cut(s) 500
HpyCH4V TGCA 5 cut(s) 20, 328, 423, 476, 593
HpyF10VI GCNNNNNNNGC 1 cut(s) 375
HpySE526I ACGT 1 cut(s) 500
Hsp92II CATG 2 cut(s) 300, 593
HspAI GCGC 1 cut(s) 176
KpnI GGTACC 1 cut(s) 186
Kzo9I GATC 1 cut(s) 219
Lsp1109I GCAGC 2 cut(s) 583, 605
LweI GCATC 2 cut(s) 315, 349
MaeI CTAG 1 cut(s) 149
MaeII ACGT 1 cut(s) 500
MaeIII GTNAC 4 cut(s) 58, 223, 258, 349
MalI GATC 1 cut(s) 221
MboI GATC 1 cut(s) 219
MboII GAAGA 1 cut(s) 89
MhlI GDGCHC 1 cut(s) 371
MluCI AATT 2 cut(s) 391, 526
MlyI GAGTC 1 cut(s) 99
MnlI CCTC 5 cut(s) 62, 214, 218, 242, 419
MseI TTAA 1 cut(s) 318
MspA1I CMGCKG 1 cut(s) 596
MspI CCGG 2 cut(s) 42, 265
MspR9I CCNGG 3 cut(s) 102, 265, 266
MvaI CCWGG 1 cut(s) 102
MwoI GCNNNNNNNGC 1 cut(s) 375
NciI CCSGG 2 cut(s) 265, 266
NdeII GATC 1 cut(s) 219
NlaIII CATG 2 cut(s) 300, 593
NlaIV GGNNCC 2 cut(s) 36, 184
NmeAIII GCCGAG 1 cut(s) 213
NmuCI GTSAC 2 cut(s) 58, 349
PceI AGGCCT 1 cut(s) 231
PfeI GAWTC 2 cut(s) 4, 105
PkrI GCNGC 2 cut(s) 595, 598
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
Psp6I CCWGG 1 cut(s) 100
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 2 cut(s) 36, 184
PspPI GGNCC 1 cut(s) 403
PvuII CAGCTG 1 cut(s) 596
RsaI GTAC 3 cut(s) 72, 184, 455
RsaNI GTAC 3 cut(s) 71, 183, 454
SaqAI TTAA 1 cut(s) 318
SatI GCNGC 2 cut(s) 594, 597
Sau3AI GATC 1 cut(s) 219
Sau96I GGNCC 1 cut(s) 403
SchI GAGTC 1 cut(s) 99
ScrFI CCNGG 3 cut(s) 102, 265, 266
SduI GDGCHC 1 cut(s) 371
SetI ASST 3 cut(s) 103, 503, 598
SfaNI GCATC 2 cut(s) 315, 349
SgrAI CRCCGGYG 1 cut(s) 41
SinI GGWCC 1 cut(s) 403
SmaI CCCGGG 1 cut(s) 266
SpeI ACTAGT 1 cut(s) 148
Sse9I AATT 2 cut(s) 391, 526
SseBI AGGCCT 1 cut(s) 231
SsiI CCGC 3 cut(s) 114, 166, 551
SspMI CTAG 1 cut(s) 149
StuI AGGCCT 1 cut(s) 231
StyD4I CCNGG 3 cut(s) 100, 263, 264
TaaI ACNGT 2 cut(s) 64, 289
TaiI ACGT 1 cut(s) 503
TaqI TCGA 3 cut(s) 75, 210, 357
TaqII GACCGA 1 cut(s) 420
TasI AATT 2 cut(s) 391, 526
TfiI GAWTC 2 cut(s) 4, 105
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TscAI CASTG 1 cut(s) 27
TseFI GTSAC 2 cut(s) 58, 349
TseI GCWGC 2 cut(s) 593, 596
Tsp45I GTSAC 2 cut(s) 58, 349
TspDTI ATGAA 1 cut(s) 17
TspMI CCCGGG 1 cut(s) 264
TspRI CASTG 1 cut(s) 27
VpaK11BI GGWCC 1 cut(s) 403
XapI RAATTY 1 cut(s) 526
XcmI CCANNNNNNNNNTGG 1 cut(s) 445
XmaI CCCGGG 1 cut(s) 264
XmiI GTMKAC 2 cut(s) 136, 539
XspI CTAG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.