Rh6DG139200

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0013608
Physical Location & Seq
Reverse (-)
20011238 .. 20011837
600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG139200.1

Sequence Viewer

Length: 600 bp
ATGGTTTCTGCACTGATTTTATTGATTCCCACCGTCATAGTCCTCTGTGTCATAATTGGTGTACTTGAAGAGAAAATCAGAGTCGCCAGACCTGGATTCGCCCCCGCCATAGATATCACCGTGAGTATACTCATGATACTAGTTATTTTTCTTGTCCGCACCATTGCGCTTGGTACCATTTCCATTGTTGAACCCTCGCTCGATTACAGGATCGTAAAAGGCATCGGATATGGATTTTATATCCCAATGTTACCCTGCGTATATGACATAATAAGCAACGTTGGCTCATGCTGTTCAAGAACAATGGTTAAACTGATGCACAATGCGAATTTTCTTATGGTGACAATAGGTGTCAAGAGCCCTGAAGGGTATGCGATTTTCAGTTATCAAATGGACCAAGAAAAGGGGTTCTGCAGAGGCGAGTGTGCTATTTGCTTGATGGAGTACGAGATAAAAGACAATTGTGCATTGTTAGATAAATGTGGACATACGTTTCACCACAAATGCTTATATGAGAATTTGGCTATGTCTACCCGTTGTCCGCTTTGTCGTCAAAGTATCAAAGATGGTAAGGAACTCCATGTAGCTGCTAGTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

199

Amino Acids

22.06

Weight (kDa)

7.48

Isoelectric Point (pI)

35.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 138 - 184 8.2e-06 RING-H2 zinc finger domain
zf-RING_2 PF13639 141 - 184 2.7e-12 Ring finger domain
zf-RING_11 PF17123 141 - 170 1.5e-07 RING-like zinc finger
zf-C3HC4_2 PF13923 141 - 183 2.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 141 - 185 5.2e-06 zinc-RING finger domain
zf-RING_UBOX PF13445 142 - 181 7.4e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 173
AccB1I GGYRCC 1 cut(s) 173
AccI GTMKAC 2 cut(s) 127, 530
AciI CCGC 3 cut(s) 105, 157, 542
AclI AACGTT 1 cut(s) 279
AclWI GGATC 1 cut(s) 218
AcsI RAATTY 2 cut(s) 328, 517
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 3 cut(s) 63, 175, 446
AfiI CCNNNNNNNGG 1 cut(s) 403
AgsI TTSAA 3 cut(s) 68, 191, 297
AhlI ACTAGT 1 cut(s) 139
AjnI CCWGG 1 cut(s) 91
AluBI AGCT 1 cut(s) 587
AluI AGCT 1 cut(s) 587
AlwI GGATC 1 cut(s) 218
ApeKI GCWGC 1 cut(s) 587
ApoI RAATTY 2 cut(s) 328, 517
Asp718I GGTACC 1 cut(s) 173
AspLEI GCGC 1 cut(s) 169
AspS9I GGNCC 1 cut(s) 394
AsuHPI GGTGA 3 cut(s) 109, 352, 488
AvaII GGWCC 1 cut(s) 394
BanI GGYRCC 1 cut(s) 173
BanII GRGCYC 1 cut(s) 362
BbvI GCAGC 1 cut(s) 574
BccI CCATC 2 cut(s) 433, 560
BciT130I CCWGG 1 cut(s) 93
BcuI ACTAGT 1 cut(s) 139
BfaI CTAG 2 cut(s) 140, 591
BfmI CTRYAG 1 cut(s) 412
BisI GCNGC 1 cut(s) 588
BlsI GCNGC 1 cut(s) 589
Bme1390I CCNGG 1 cut(s) 93
Bme18I GGWCC 1 cut(s) 394
BmgT120I GGNCC 1 cut(s) 394
BmiI GGNNCC 1 cut(s) 175
BmrFI CCNGG 1 cut(s) 93
BmsI GCATC 2 cut(s) 231, 306
Bsc4I CCNNNNNNNGG 1 cut(s) 403
Bse3DI GCAATG 1 cut(s) 162
BseBI CCWGG 1 cut(s) 93
BseLI CCNNNNNNNGG 1 cut(s) 403
BseMI GCAATG 1 cut(s) 162
BseXI GCAGC 1 cut(s) 574
BshNI GGYRCC 1 cut(s) 173
BslI CCNNNNNNNGG 1 cut(s) 403
Bsp1286I GDGCHC 1 cut(s) 362
Bsp143I GATC 1 cut(s) 210
BspACI CCGC 3 cut(s) 105, 157, 542
BspHI TCATGA 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 175
BspMAI CTGCAG 1 cut(s) 416
BspPI GGATC 1 cut(s) 218
BspT107I GGYRCC 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 162
BssMI GATC 1 cut(s) 210
BssNAI GTATAC 1 cut(s) 128
Bst1107I GTATAC 1 cut(s) 128
Bst2UI CCWGG 1 cut(s) 93
Bst4CI ACNGT 2 cut(s) 34, 121
Bst6I CTCTTC 1 cut(s) 63
BstHHI GCGC 1 cut(s) 169
BstKTI GATC 1 cut(s) 213
BstMBI GATC 1 cut(s) 210
BstMWI GCNNNNNNNGC 1 cut(s) 282
BstNI CCWGG 1 cut(s) 93
BstSCI CCNGG 1 cut(s) 91
BstSFI CTRYAG 1 cut(s) 412
BstV1I GCAGC 1 cut(s) 574
BstZ17I GTATAC 1 cut(s) 128
BtsIMutI CAGTG 1 cut(s) 11
CciI TCATGA 1 cut(s) 132
CfoI GCGC 1 cut(s) 169
Cfr13I GGNCC 1 cut(s) 394
Csp6I GTAC 3 cut(s) 62, 174, 445
CviAII CATG 3 cut(s) 133, 288, 581
CviJI RGCY 4 cut(s) 285, 360, 524, 587
CviKI_1 RGCY 4 cut(s) 285, 360, 524, 587
CviQI GTAC 3 cut(s) 62, 174, 445
DpnI GATC 1 cut(s) 212
DpnII GATC 1 cut(s) 210
Eam1104I CTCTTC 1 cut(s) 63
EarI CTCTTC 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 362
Eco32I GATATC 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 394
Eco57I CTGAAG 1 cut(s) 384
EcoRII CCWGG 1 cut(s) 91
EcoRV GATATC 1 cut(s) 115
EcoT38I GRGCYC 1 cut(s) 362
FaeI CATG 3 cut(s) 136, 291, 584
FatI CATG 3 cut(s) 132, 287, 580
FauI CCCGC 1 cut(s) 112
FblI GTMKAC 2 cut(s) 127, 530
Fnu4HI GCNGC 1 cut(s) 588
FriOI GRGCYC 1 cut(s) 362
Fsp4HI GCNGC 1 cut(s) 588
FspBI CTAG 2 cut(s) 140, 591
GlaI GCGC 1 cut(s) 168
GluI GCNGC 1 cut(s) 588
HhaI GCGC 1 cut(s) 169
Hin1II CATG 3 cut(s) 136, 291, 584
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HinfI GANTC 3 cut(s) 25, 81, 96
HphI GGTGA 3 cut(s) 109, 352, 488
Hpy166II GTNNAC 4 cut(s) 62, 128, 485, 531
Hpy188I TCNGA 2 cut(s) 80, 227
Hpy188III TCNNGA 3 cut(s) 133, 297, 355
Hpy8I GTNNAC 4 cut(s) 62, 128, 485, 531
HpyAV CCTTC 1 cut(s) 359
HpyCH4III ACNGT 2 cut(s) 34, 121
HpyCH4IV ACGT 2 cut(s) 279, 491
HpyCH4V TGCA 4 cut(s) 11, 319, 414, 467
HpyF10VI GCNNNNNNNGC 1 cut(s) 282
HpySE526I ACGT 2 cut(s) 279, 491
Hsp92II CATG 3 cut(s) 136, 291, 584
HspAI GCGC 1 cut(s) 167
KpnI GGTACC 1 cut(s) 177
Kzo9I GATC 1 cut(s) 210
LpnPI CCDG 6 cut(s) 78, 100, 105, 193, 268, 375
Lsp1109I GCAGC 1 cut(s) 574
LweI GCATC 2 cut(s) 231, 306
MaeI CTAG 2 cut(s) 140, 591
MaeII ACGT 2 cut(s) 279, 491
MaeIII GTNAC 2 cut(s) 249, 340
MalI GATC 1 cut(s) 212
MboI GATC 1 cut(s) 210
MboII GAAGA 1 cut(s) 80
MfeI CAATTG 1 cut(s) 460
MhlI GDGCHC 1 cut(s) 362
MluCI AATT 4 cut(s) 54, 328, 460, 517
MlyI GAGTC 1 cut(s) 90
MnlI CCTC 3 cut(s) 53, 205, 410
MseI TTAA 1 cut(s) 309
MspR9I CCNGG 1 cut(s) 93
MunI CAATTG 1 cut(s) 460
MvaI CCWGG 1 cut(s) 93
MwoI GCNNNNNNNGC 1 cut(s) 282
NdeII GATC 1 cut(s) 210
NlaIII CATG 3 cut(s) 136, 291, 584
NlaIV GGNNCC 1 cut(s) 175
NmuCI GTSAC 1 cut(s) 340
PagI TCATGA 1 cut(s) 132
PfeI GAWTC 2 cut(s) 25, 96
PkrI GCNGC 1 cut(s) 589
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Psp1406I AACGTT 1 cut(s) 279
Psp6I CCWGG 1 cut(s) 91
PspGI CCWGG 1 cut(s) 91
PspN4I GGNNCC 1 cut(s) 175
PspPI GGNCC 1 cut(s) 394
PstI CTGCAG 1 cut(s) 416
RsaI GTAC 3 cut(s) 63, 175, 446
RsaNI GTAC 3 cut(s) 62, 174, 445
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 1 cut(s) 588
Sau3AI GATC 1 cut(s) 210
Sau96I GGNCC 1 cut(s) 394
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 1 cut(s) 93
SduI GDGCHC 1 cut(s) 362
SetI ASST 5 cut(s) 94, 282, 352, 494, 589
SfaNI GCATC 2 cut(s) 231, 306
SfcI CTRYAG 1 cut(s) 412
SinI GGWCC 1 cut(s) 394
SpeI ACTAGT 1 cut(s) 139
Sse9I AATT 4 cut(s) 54, 328, 460, 517
SsiI CCGC 3 cut(s) 105, 157, 542
SspMI CTAG 2 cut(s) 140, 591
StyD4I CCNGG 1 cut(s) 91
TaaI ACNGT 2 cut(s) 34, 121
TaiI ACGT 2 cut(s) 282, 494
TaqI TCGA 1 cut(s) 201
TasI AATT 4 cut(s) 54, 328, 460, 517
TatI WGTACW 1 cut(s) 61
TfiI GAWTC 2 cut(s) 25, 96
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 1 cut(s) 587
Tsp45I GTSAC 1 cut(s) 340
TspRI CASTG 1 cut(s) 18
VpaK11BI GGWCC 1 cut(s) 394
XapI RAATTY 2 cut(s) 328, 517
XmiI GTMKAC 2 cut(s) 127, 530
XspI CTAG 2 cut(s) 140, 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.