Rroxscaffold_7G00200110

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0013608
Physical Location & Seq
Reverse (-)
47404519 .. 47405560
1042 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00200110.1

Sequence Viewer

Length: 798 bp
ATGGAGTTCGAGAAAGAAGACGGGTGCACTTCGCTAGATAAATGTGGACACACATTTCACCATGTATGCCTCCAGAAGTGTTTAGTTATCTCAACATTGGTCCACTTTGTTGCCAAAGTATCAAAGATTGTACAAAAGAATAAGGAACTCGATATAGGCAGGCATCTATCTACTAAGCAACTCTGTATCATAAGTACCATGGTTTCTGCACTGATTTTATTGATTCCCACCGTCATAGTCCTCTGTGTCATAATTGGTGTACTTGAAGAGAAAATTAGAGTCGCCAGACCTGGATTCGCTCCCGCCATAGATATCACCGCGAGTATACTCATGATACTAGTTATTTTTCTTTTCCGCACCATTGCGCTTGGTACCATTTCCATTGTTGAACCCTCCCTCGATTACAGGATCGTAAAAGGCCTCGGATATGGATTTTATATCCCAATGTTACCCTGCGTATATGATATAATAAGCACCGTTGGCTCATGCTGTTCAAGAACAATGGTTAAACTGATGCACAATGCGACTTTTCTTATGGTGACAATAGGTGTCAGGAGCCCTGAAGGGTATGCGATTTTCAGTTATCAAATGGACCAAGAAAAGGGGTTTTGCAGAGGCGAGTGTGCTATTTGCTTGATGGAGTACGAGATAAAAGACAATTGTGCATTGTTAGATACATGTGGACATACGTTTCACCACAAATGCTTATATGAGAATTTGGCTATGTCTACCCGTTGTCCGCTTTGTCGTCAAAGTATCAAAGGTGGTAAGGAACTCCATGTAGCTGCTAGTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

265

Amino Acids

29.45

Weight (kDa)

8.09

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 207 - 250 5.9e-12 Ring finger domain
zf-RING_11 PF17123 207 - 236 2.1e-07 RING-like zinc finger
zf-RING_5 PF14634 207 - 251 4.8e-06 zinc-RING finger domain
zf-C3HC4_2 PF13923 207 - 249 7.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 208 - 247 7e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 371
AccB1I GGYRCC 1 cut(s) 371
AccI GTMKAC 2 cut(s) 325, 728
AccII CGCG 1 cut(s) 320
AciI CCGC 4 cut(s) 303, 318, 355, 740
AclWI GGATC 1 cut(s) 416
AcsI RAATTY 1 cut(s) 715
AcuI CTGAAG 1 cut(s) 582
AfaI GTAC 5 cut(s) 132, 196, 261, 373, 644
AfiI CCNNNNNNNGG 1 cut(s) 601
AflIII ACRYGT 1 cut(s) 677
AgsI TTSAA 3 cut(s) 266, 389, 495
AhlI ACTAGT 1 cut(s) 337
AjnI CCWGG 1 cut(s) 289
AluBI AGCT 1 cut(s) 785
AluI AGCT 1 cut(s) 785
Alw21I GWGCWC 1 cut(s) 29
Alw44I GTGCAC 1 cut(s) 25
AlwI GGATC 1 cut(s) 416
AoxI GGCC 1 cut(s) 418
ApaLI GTGCAC 1 cut(s) 25
ApeKI GCWGC 1 cut(s) 785
ApoI RAATTY 1 cut(s) 715
Asp718I GGTACC 1 cut(s) 371
AspLEI GCGC 1 cut(s) 367
AspS9I GGNCC 2 cut(s) 100, 592
AsuHPI GGTGA 4 cut(s) 50, 307, 550, 686
AvaII GGWCC 2 cut(s) 100, 592
BaeGI GKGCMC 1 cut(s) 29
BanI GGYRCC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 560
BbsI GAAGAC 1 cut(s) 24
Bbv12I GWGCWC 1 cut(s) 29
BbvI GCAGC 1 cut(s) 772
BccI CCATC 1 cut(s) 631
BciT130I CCWGG 1 cut(s) 291
BcuI ACTAGT 1 cut(s) 337
BfaI CTAG 3 cut(s) 35, 338, 789
BisI GCNGC 1 cut(s) 786
BlsI GCNGC 1 cut(s) 787
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 2 cut(s) 100, 592
BmgT120I GGNCC 2 cut(s) 100, 592
BmiI GGNNCC 2 cut(s) 373, 557
BmrFI CCNGG 1 cut(s) 291
BmsI GCATC 2 cut(s) 172, 504
BpiI GAAGAC 1 cut(s) 24
BpmI CTGGAG 1 cut(s) 56
BsaJI CCNNGG 2 cut(s) 198, 421
Bsc4I CCNNNNNNNGG 1 cut(s) 601
Bse3DI GCAATG 1 cut(s) 360
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 198, 421
BseLI CCNNNNNNNGG 1 cut(s) 601
BseMI GCAATG 1 cut(s) 360
BseSI GKGCMC 1 cut(s) 29
BseXI GCAGC 1 cut(s) 772
BsgI GTGCAG 1 cut(s) 192
Bsh1236I CGCG 1 cut(s) 320
BshFI GGCC 1 cut(s) 420
BshNI GGYRCC 1 cut(s) 371
BsiHKAI GWGCWC 1 cut(s) 29
BslI CCNNNNNNNGG 1 cut(s) 601
BsnI GGCC 1 cut(s) 420
Bsp1286I GDGCHC 2 cut(s) 29, 560
Bsp1407I TGTACA 1 cut(s) 130
Bsp143I GATC 1 cut(s) 408
Bsp19I CCATGG 1 cut(s) 198
BspACI CCGC 4 cut(s) 303, 318, 355, 740
BspANI GGCC 1 cut(s) 420
BspFNI CGCG 1 cut(s) 320
BspHI TCATGA 1 cut(s) 330
BspLI GGNNCC 2 cut(s) 373, 557
BspPI GGATC 1 cut(s) 416
BspT107I GGYRCC 1 cut(s) 371
BsrDI GCAATG 1 cut(s) 360
BsrGI TGTACA 1 cut(s) 130
BssECI CCNNGG 2 cut(s) 198, 421
BssMI GATC 1 cut(s) 408
BssNAI GTATAC 1 cut(s) 326
BssT1I CCWWGG 1 cut(s) 198
Bst1107I GTATAC 1 cut(s) 326
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 2 cut(s) 232, 478
Bst6I CTCTTC 1 cut(s) 261
BstAUI TGTACA 1 cut(s) 130
BstC8I GCNNGC 1 cut(s) 161
BstDEI CTNAG 1 cut(s) 174
BstDSI CCRYGG 1 cut(s) 198
BstFNI CGCG 1 cut(s) 320
BstHHI GCGC 1 cut(s) 367
BstKTI GATC 1 cut(s) 411
BstMBI GATC 1 cut(s) 408
BstMWI GCNNNNNNNGC 1 cut(s) 480
BstNI CCWGG 1 cut(s) 291
BstNSI RCATGY 1 cut(s) 681
BstSCI CCNGG 1 cut(s) 289
BstSLI GKGCMC 1 cut(s) 29
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 1 cut(s) 772
BstV2I GAAGAC 1 cut(s) 24
BstZ17I GTATAC 1 cut(s) 326
BsuRI GGCC 1 cut(s) 420
BtgI CCRYGG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 209
Cac8I GCNNGC 1 cut(s) 161
CciI TCATGA 1 cut(s) 330
CfoI GCGC 1 cut(s) 367
Cfr13I GGNCC 2 cut(s) 100, 592
Csp6I GTAC 5 cut(s) 131, 195, 260, 372, 643
CviAII CATG 6 cut(s) 62, 199, 331, 486, 678, 779
CviJI RGCY 5 cut(s) 420, 483, 558, 722, 785
CviKI_1 RGCY 5 cut(s) 420, 483, 558, 722, 785
CviQI GTAC 5 cut(s) 131, 195, 260, 372, 643
DdeI CTNAG 1 cut(s) 174
DpnI GATC 1 cut(s) 410
DpnII GATC 1 cut(s) 408
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Eco130I CCWWGG 1 cut(s) 198
Eco147I AGGCCT 1 cut(s) 420
Eco24I GRGCYC 1 cut(s) 560
Eco32I GATATC 1 cut(s) 313
Eco47I GGWCC 2 cut(s) 100, 592
Eco57I CTGAAG 1 cut(s) 582
EcoRII CCWGG 1 cut(s) 289
EcoRV GATATC 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 198
EcoT38I GRGCYC 1 cut(s) 560
ErhI CCWWGG 1 cut(s) 198
FaeI CATG 6 cut(s) 65, 202, 334, 489, 681, 782
FatI CATG 6 cut(s) 61, 198, 330, 485, 677, 778
FauI CCCGC 1 cut(s) 310
FblI GTMKAC 2 cut(s) 325, 728
Fnu4HI GCNGC 1 cut(s) 786
FriOI GRGCYC 1 cut(s) 560
Fsp4HI GCNGC 1 cut(s) 786
FspBI CTAG 3 cut(s) 35, 338, 789
GlaI GCGC 1 cut(s) 366
GluI GCNGC 1 cut(s) 786
GsuI CTGGAG 1 cut(s) 56
HaeIII GGCC 1 cut(s) 420
HhaI GCGC 1 cut(s) 367
Hin1II CATG 6 cut(s) 65, 202, 334, 489, 681, 782
Hin6I GCGC 1 cut(s) 365
HinP1I GCGC 1 cut(s) 365
HinfI GANTC 3 cut(s) 223, 279, 294
HphI GGTGA 4 cut(s) 50, 307, 550, 686
Hpy166II GTNNAC 7 cut(s) 27, 47, 103, 260, 326, 683, 729
Hpy188I TCNGA 1 cut(s) 425
Hpy188III TCNNGA 5 cut(s) 10, 73, 331, 495, 553
Hpy8I GTNNAC 7 cut(s) 27, 47, 103, 260, 326, 683, 729
HpyAV CCTTC 1 cut(s) 557
HpyCH4III ACNGT 2 cut(s) 232, 478
HpyCH4IV ACGT 1 cut(s) 689
HpyCH4V TGCA 5 cut(s) 27, 209, 517, 612, 665
HpyF10VI GCNNNNNNNGC 1 cut(s) 480
HpyF3I CTNAG 1 cut(s) 174
HpySE526I ACGT 1 cut(s) 689
Hsp92II CATG 6 cut(s) 65, 202, 334, 489, 681, 782
HspAI GCGC 1 cut(s) 365
KpnI GGTACC 1 cut(s) 375
Kzo9I GATC 1 cut(s) 408
LmnI GCTCC 2 cut(s) 304, 555
LpnPI CCDG 9 cut(s) 86, 145, 276, 298, 303, 391, 466, 538, 573
Lsp1109I GCAGC 1 cut(s) 772
LweI GCATC 2 cut(s) 172, 504
MaeI CTAG 3 cut(s) 35, 338, 789
MaeII ACGT 1 cut(s) 689
MaeIII GTNAC 2 cut(s) 447, 538
MalI GATC 1 cut(s) 410
MboI GATC 1 cut(s) 408
MboII GAAGA 2 cut(s) 29, 278
MfeI CAATTG 1 cut(s) 658
MhlI GDGCHC 2 cut(s) 29, 560
MluCI AATT 4 cut(s) 252, 273, 658, 715
MlyI GAGTC 1 cut(s) 288
MnlI CCTC 6 cut(s) 80, 251, 403, 407, 431, 608
MseI TTAA 1 cut(s) 507
MspR9I CCNGG 1 cut(s) 291
MunI CAATTG 1 cut(s) 658
MvaI CCWGG 1 cut(s) 291
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 1 cut(s) 480
NcoI CCATGG 1 cut(s) 198
NdeII GATC 1 cut(s) 408
NlaIII CATG 6 cut(s) 65, 202, 334, 489, 681, 782
NlaIV GGNNCC 2 cut(s) 373, 557
NmuCI GTSAC 1 cut(s) 538
NspI RCATGY 1 cut(s) 681
PagI TCATGA 1 cut(s) 330
PceI AGGCCT 1 cut(s) 420
PciI ACATGT 1 cut(s) 677
PfeI GAWTC 2 cut(s) 223, 294
PkrI GCNGC 1 cut(s) 787
PleI GAGTC 1 cut(s) 287
PpsI GAGTC 1 cut(s) 287
PscI ACATGT 1 cut(s) 677
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 2 cut(s) 373, 557
PspPI GGNCC 2 cut(s) 100, 592
RsaI GTAC 5 cut(s) 132, 196, 261, 373, 644
RsaNI GTAC 5 cut(s) 131, 195, 260, 372, 643
SaqAI TTAA 1 cut(s) 507
SatI GCNGC 1 cut(s) 786
Sau3AI GATC 1 cut(s) 408
Sau96I GGNCC 2 cut(s) 100, 592
SchI GAGTC 1 cut(s) 288
ScrFI CCNGG 1 cut(s) 291
SduI GDGCHC 2 cut(s) 29, 560
SetI ASST 5 cut(s) 292, 550, 692, 766, 787
SfaNI GCATC 2 cut(s) 172, 504
SinI GGWCC 2 cut(s) 100, 592
SpeI ACTAGT 1 cut(s) 337
Sse9I AATT 4 cut(s) 252, 273, 658, 715
SseBI AGGCCT 1 cut(s) 420
SsiI CCGC 4 cut(s) 303, 318, 355, 740
SspMI CTAG 3 cut(s) 35, 338, 789
StuI AGGCCT 1 cut(s) 420
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 1 cut(s) 198
TaaI ACNGT 2 cut(s) 232, 478
TaiI ACGT 1 cut(s) 692
TaqI TCGA 3 cut(s) 9, 150, 399
TasI AATT 4 cut(s) 252, 273, 658, 715
TatI WGTACW 2 cut(s) 130, 259
TfiI GAWTC 2 cut(s) 223, 294
Tru1I TTAA 1 cut(s) 507
Tru9I TTAA 1 cut(s) 507
TscAI CASTG 1 cut(s) 216
TseFI GTSAC 1 cut(s) 538
TseI GCWGC 1 cut(s) 785
Tsp45I GTSAC 1 cut(s) 538
TspRI CASTG 1 cut(s) 216
VneI GTGCAC 1 cut(s) 25
VpaK11BI GGWCC 2 cut(s) 100, 592
XapI RAATTY 1 cut(s) 715
XceI RCATGY 1 cut(s) 681
XmiI GTMKAC 2 cut(s) 325, 728
XspI CTAG 3 cut(s) 35, 338, 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.