FvH4_2g12231

Transmembrane protein 18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015433
Physical Location & Seq
Forward (+)
10742243 .. 10745097
2855 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g12231.t1

Sequence Viewer

Length: 492 bp
ATGGAGCAGATTCAGTCGGCTTTGGAAGCTCACCTGGACCAGATGGCGGATCTTGTCCACACAGTTTCCGCGGACTTCCGCTCTGGTCTTGGACCCGCTTTCGATAACTTGATCGGTTTCTTCCATGCTATCAACTGGAAGGAAACATGGTTGATGTGCTTATTGGCATTCCACTTTCTATATTTGGTAACCGCCATTATCTCCAGAAAGAATCTTAACTTCCAAATGCTTTTGTTCCTTACCGCCTTGGCTGGAGTATATCTTGCTGAGAGGTTCAATGGTTTACTGCGCCAGAACTGGAGAAGTTTCGCCAGCCAGAATTACTTTGATTCTAATGGAGTTTTTGTGTCAGTACTTTGGTCGGGGCCTCTTCTTGTGATTGCAATCATAATCTTGATAAACACGCTCTTTTCCCTATGTCATCTGATTGTCAAGTGGAAAAAAGCTGAGCTAAGACACCGTGCACGACTTTCCCAGAATAAGCAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM18

Protein Analysis

164

Amino Acids

18.74

Weight (kDa)

9.06

Isoelectric Point (pI)

32.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM18 PF14770 39 - 156 5.4e-45 Transmembrane protein 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015433)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34350 AT1G34350
fragaria_vesca FvH4_2g12231
malus_domestica MD05G1040400.v1.1 MD10G1046500.v1.1
prunus_persica Prupe.8G054100_v2.0.a1
pyrus_communis pycom05g03300 pycom10g03270
rosa_chinensis RchiOBHm_Chr6g0272201
rosa_laevigata RLG00000013692
rosa_multiflora Rmu_sc0005748.1_g000009
rosa_roxburghii Rroxscaffold_7G00196500
rosa_rugosa Rorug06G0067100
rosa_samantha Rh6AG183600
rosa_wichuraiana Rw0G003850 Rw6G015830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 81
AccII CGCG 1 cut(s) 71
AciI CCGC 7 cut(s) 47, 69, 71, 79, 96, 192, 243
AclWI GGATC 1 cut(s) 57
AfaI GTAC 1 cut(s) 354
AfiI CCNNNNNNNGG 1 cut(s) 46
AgsI TTSAA 1 cut(s) 277
AjnI CCWGG 1 cut(s) 33
AluBI AGCT 3 cut(s) 29, 446, 451
AluI AGCT 3 cut(s) 29, 446, 451
Alw21I GWGCWC 1 cut(s) 466
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 1 cut(s) 57
AoxI GGCC 1 cut(s) 365
ApaLI GTGCAC 1 cut(s) 462
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 3 cut(s) 37, 92, 365
AsuHPI GGTGA 1 cut(s) 23
AvaII GGWCC 2 cut(s) 37, 92
BaeGI GKGCMC 1 cut(s) 466
Bbv12I GWGCWC 1 cut(s) 466
BccI CCATC 1 cut(s) 37
BciT130I CCWGG 1 cut(s) 35
BlpI GCTNAGC 1 cut(s) 447
BmcAI AGTACT 1 cut(s) 354
Bme1390I CCNGG 1 cut(s) 35
Bme18I GGWCC 2 cut(s) 37, 92
BmgT120I GGNCC 3 cut(s) 37, 92, 365
BmiI GGNNCC 2 cut(s) 94, 366
BmrFI CCNGG 1 cut(s) 35
BpmI CTGGAG 3 cut(s) 187, 273, 319
Bpu1102I GCTNAGC 1 cut(s) 447
BsaBI GATNNNNATC 1 cut(s) 383
BsaJI CCNNGG 2 cut(s) 69, 246
BsaXI ACNNNNNCTCC 2 cut(s) 330, 360
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse1I ACTGG 2 cut(s) 140, 302
Bse8I GATNNNNATC 1 cut(s) 383
BseBI CCWGG 1 cut(s) 35
BseDI CCNNGG 2 cut(s) 69, 246
BseJI GATNNNNATC 1 cut(s) 383
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMII CTCAG 2 cut(s) 258, 438
BseNI ACTGG 2 cut(s) 140, 302
BseSI GKGCMC 1 cut(s) 466
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 1 cut(s) 367
BsiHKAI GWGCWC 1 cut(s) 466
BslI CCNNNNNNNGG 1 cut(s) 46
BsmI GAATGC 1 cut(s) 167
BsnI GGCC 1 cut(s) 367
Bsp1286I GDGCHC 1 cut(s) 466
Bsp143I GATC 2 cut(s) 49, 111
Bsp1720I GCTNAGC 1 cut(s) 447
BspACI CCGC 7 cut(s) 47, 69, 71, 79, 96, 192, 243
BspANI GGCC 1 cut(s) 367
BspCNI CTCAG 2 cut(s) 259, 439
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 2 cut(s) 94, 366
BspPI GGATC 1 cut(s) 57
BsrBI CCGCTC 1 cut(s) 81
BsrI ACTGG 2 cut(s) 140, 302
BssECI CCNNGG 2 cut(s) 69, 246
BssMI GATC 2 cut(s) 49, 111
BssT1I CCWWGG 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 35
Bst4CI ACNGT 2 cut(s) 64, 461
Bst6I CTCTTC 1 cut(s) 375
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 3 cut(s) 267, 447, 452
BstDSI CCRYGG 1 cut(s) 69
BstEII GGTNACC 1 cut(s) 187
BstFNI CGCG 1 cut(s) 71
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 2 cut(s) 52, 114
BstMBI GATC 2 cut(s) 49, 111
BstMWI GCNNNNNNNGC 1 cut(s) 26
BstNI CCWGG 1 cut(s) 35
BstPI GGTNACC 1 cut(s) 187
BstSCI CCNGG 1 cut(s) 33
BstSLI GKGCMC 1 cut(s) 466
BstUI CGCG 1 cut(s) 71
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BsuRI GGCC 1 cut(s) 367
BtgI CCRYGG 1 cut(s) 69
Cac8I GCNNGC 1 cut(s) 313
CfoI GCGC 1 cut(s) 291
Cfr13I GGNCC 3 cut(s) 37, 92, 365
Cfr42I CCGCGG 1 cut(s) 72
Csp6I GTAC 1 cut(s) 353
CviAII CATG 2 cut(s) 125, 147
CviJI RGCY 7 cut(s) 20, 29, 251, 315, 367, 446, 451
CviKI_1 RGCY 7 cut(s) 20, 29, 251, 315, 367, 446, 451
CviQI GTAC 1 cut(s) 353
DdeI CTNAG 3 cut(s) 267, 447, 452
DpnI GATC 2 cut(s) 51, 113
DpnII GATC 2 cut(s) 49, 111
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
EciI GGCGGA 1 cut(s) 62
Eco130I CCWWGG 1 cut(s) 246
Eco47I GGWCC 2 cut(s) 37, 92
Eco91I GGTNACC 1 cut(s) 187
EcoO109I RGGNCCY 1 cut(s) 365
EcoO65I GGTNACC 1 cut(s) 187
EcoRII CCWGG 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 2 cut(s) 128, 150
FaiI YATR 6 cut(s) 126, 148, 181, 259, 389, 418
FatI CATG 2 cut(s) 124, 146
FauI CCCGC 1 cut(s) 103
GlaI GCGC 1 cut(s) 290
GsuI CTGGAG 3 cut(s) 187, 273, 319
HaeIII GGCC 1 cut(s) 367
HhaI GCGC 1 cut(s) 291
Hin1II CATG 2 cut(s) 128, 150
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HinfI GANTC 3 cut(s) 10, 211, 329
HphI GGTGA 1 cut(s) 23
Hpy166II GTNNAC 3 cut(s) 58, 284, 464
Hpy188I TCNGA 1 cut(s) 426
Hpy188III TCNNGA 2 cut(s) 204, 394
Hpy8I GTNNAC 3 cut(s) 58, 284, 464
HpyAV CCTTC 1 cut(s) 133
HpyCH4III ACNGT 2 cut(s) 64, 461
HpyCH4V TGCA 2 cut(s) 383, 464
HpyF10VI GCNNNNNNNGC 1 cut(s) 26
HpyF3I CTNAG 3 cut(s) 267, 447, 452
Hsp92II CATG 2 cut(s) 128, 150
HspAI GCGC 1 cut(s) 289
KspI CCGCGG 1 cut(s) 72
Kzo9I GATC 2 cut(s) 49, 111
LmnI GCTCC 1 cut(s) 4
MaeIII GTNAC 1 cut(s) 187
MalI GATC 2 cut(s) 51, 113
MbiI CCGCTC 1 cut(s) 81
MboI GATC 2 cut(s) 49, 111
MboII GAAGA 2 cut(s) 112, 362
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 1 cut(s) 466
MluCI AATT 1 cut(s) 319
MnlI CCTC 2 cut(s) 264, 378
MseI TTAA 1 cut(s) 216
MspA1I CMGCKG 1 cut(s) 71
MspR9I CCNGG 1 cut(s) 35
Mva1269I GAATGC 1 cut(s) 167
MvaI CCWGG 1 cut(s) 35
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 1 cut(s) 26
NdeII GATC 2 cut(s) 49, 111
NlaIII CATG 2 cut(s) 128, 150
NlaIV GGNNCC 2 cut(s) 94, 366
PctI GAATGC 1 cut(s) 167
PfeI GAWTC 3 cut(s) 10, 211, 329
Psp6I CCWGG 1 cut(s) 33
PspEI GGTNACC 1 cut(s) 187
PspGI CCWGG 1 cut(s) 33
PspN4I GGNNCC 2 cut(s) 94, 366
PspPI GGNCC 3 cut(s) 37, 92, 365
PsuI RGATCY 1 cut(s) 49
RsaI GTAC 1 cut(s) 354
RsaNI GTAC 1 cut(s) 353
SacII CCGCGG 1 cut(s) 72
SaqAI TTAA 1 cut(s) 216
Sau3AI GATC 2 cut(s) 49, 111
Sau96I GGNCC 3 cut(s) 37, 92, 365
ScaI AGTACT 1 cut(s) 354
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 1 cut(s) 466
SetI ASST 5 cut(s) 31, 36, 275, 448, 453
Sfr303I CCGCGG 1 cut(s) 72
SgrBI CCGCGG 1 cut(s) 72
SinI GGWCC 2 cut(s) 37, 92
Sse9I AATT 1 cut(s) 319
SsiI CCGC 7 cut(s) 47, 69, 71, 79, 96, 192, 243
StyD4I CCNGG 1 cut(s) 33
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 2 cut(s) 64, 461
TaqI TCGA 1 cut(s) 102
TasI AATT 1 cut(s) 319
TatI WGTACW 1 cut(s) 352
TfiI GAWTC 3 cut(s) 10, 211, 329
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
VneI GTGCAC 1 cut(s) 462
VpaK11BI GGWCC 2 cut(s) 37, 92
ZrmI AGTACT 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.