Rorug06G0067100

Transmembrane protein 18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0015433
Physical Location & Seq
Forward (+)
8863867 .. 8864961
1095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0067100.1

Sequence Viewer

Length: 681 bp
ATGTCTCAAAACAATGTGTTTCTCCTCTCCCTTCTCATAACACTTGCAGGCCTTCATGCCGCCCATGCAGTCGACTACACTGTCACAAACAACGCCGGATCAAGCGCCGGGGCTGTCCGATTTAACAGAGACATCGGAGCAGACTACAGCAAGAGAACCCTTATCTCCACGGCCAATTTCATATGGAAGATCTCCCAGCAAAGCGCAGCAGCAGATAGAAAGAGCGTATCCAAAGTCAGCTTGTTTATTGACAACATAGACGGCGTGGCGTATACTAGCAACAACCAGATTCATGTTAGCGCCAGCTACATAGCAGGCTACTCCGGCGACGTGAAAAAGGAGATAACGGGTGTGCTCTACCACGAATCGACGCACATATGGCAGTGGAATGGCAATGGTAAAGCCCCAGGTGGGTTGATTGAAGGAATTGCAGACTTTGTGAGGTTGAAGGCTGGGTATGCACCGAGTCACTGGGTTAAGCCAGGTCAAGGGGATAGGTGGGATCAGGGTTATGATGTTACTGCTAGGTTTTTGGATTACTGTAATAGTTTGAAAGCTGGGTTTATTGCAGAGTTGAACAAGAAGTTGAAGACTGGTTATAGTGTTAACTACTTTGCTGATATTTTGGGAAAGAACGTTAATCAGCTCTGGGCTGATTACAAGGCTAAGTATGGCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM18

Protein Analysis

226

Amino Acids

24.78

Weight (kDa)

9.23

Isoelectric Point (pI)

20.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSP PF04450 23 - 220 7.5e-78 Peptidase of plants and bacteria
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015433)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34350 AT1G34350
fragaria_vesca FvH4_2g12231
malus_domestica MD05G1040400.v1.1 MD10G1046500.v1.1
prunus_persica Prupe.8G054100_v2.0.a1
pyrus_communis pycom05g03300 pycom10g03270
rosa_chinensis RchiOBHm_Chr6g0272201
rosa_laevigata RLG00000013692
rosa_multiflora Rmu_sc0005748.1_g000009
rosa_roxburghii Rroxscaffold_7G00196500
rosa_rugosa Rorug06G0067100
rosa_samantha Rh6AG183600
rosa_wichuraiana Rw0G003850 Rw6G015830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 80
AccI GTMKAC 2 cut(s) 72, 272
AciI CCGC 1 cut(s) 60
AclI AACGTT 1 cut(s) 636
AclWI GGATC 2 cut(s) 106, 510
AcoI YGGCCR 1 cut(s) 171
AfiI CCNNNNNNNGG 2 cut(s) 411, 488
AgsI TTSAA 5 cut(s) 422, 448, 553, 577, 589
AjiI CACGTC 1 cut(s) 331
AjnI CCWGG 2 cut(s) 406, 481
AluBI AGCT 4 cut(s) 240, 306, 557, 646
AluI AGCT 4 cut(s) 240, 306, 557, 646
Alw21I GWGCWC 1 cut(s) 357
Alw26I GTCTC 2 cut(s) 9, 123
AlwI GGATC 2 cut(s) 106, 510
AoxI GGCC 2 cut(s) 49, 171
ApeKI GCWGC 2 cut(s) 206, 209
AspLEI GCGC 3 cut(s) 107, 206, 302
AsuC2I CCSGG 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 596
Bbv12I GWGCWC 1 cut(s) 357
BbvI GCAGC 2 cut(s) 218, 221
BceAI ACGGC 2 cut(s) 186, 277
BciT130I CCWGG 2 cut(s) 408, 483
BciVI GTATCC 1 cut(s) 238
BcnI CCSGG 1 cut(s) 109
BcoDI GTCTC 2 cut(s) 9, 123
BfaI CTAG 2 cut(s) 276, 525
BfmI CTRYAG 1 cut(s) 145
BfoI RGCGCY 2 cut(s) 108, 303
BfuI GTATCC 1 cut(s) 238
BglII AGATCT 1 cut(s) 189
BisI GCNGC 3 cut(s) 60, 207, 210
BlsI GCNGC 3 cut(s) 61, 208, 211
Bme1390I CCNGG 3 cut(s) 109, 408, 483
BmgBI CACGTC 1 cut(s) 331
BmrFI CCNGG 3 cut(s) 109, 408, 483
BmrI ACTGGG 1 cut(s) 481
BmuI ACTGGG 1 cut(s) 481
BpiI GAAGAC 1 cut(s) 596
BpuMI CCSGG 1 cut(s) 109
BsaJI CCNNGG 3 cut(s) 108, 168, 406
Bsc4I CCNNNNNNNGG 2 cut(s) 411, 488
Bse1I ACTGG 2 cut(s) 476, 598
Bse3DI GCAATG 1 cut(s) 400
BseBI CCWGG 2 cut(s) 408, 483
BseDI CCNNGG 3 cut(s) 108, 168, 406
BseLI CCNNNNNNNGG 2 cut(s) 411, 488
BseMI GCAATG 1 cut(s) 400
BseNI ACTGG 2 cut(s) 476, 598
BseRI GAGGAG 1 cut(s) 14
BseXI GCAGC 2 cut(s) 218, 221
BseYI CCCAGC 3 cut(s) 195, 452, 557
BshFI GGCC 2 cut(s) 51, 173
BsiHKAI GWGCWC 1 cut(s) 357
BsiSI CCGG 3 cut(s) 96, 108, 324
BslI CCNNNNNNNGG 2 cut(s) 411, 488
BsmAI GTCTC 2 cut(s) 9, 123
BsnI GGCC 2 cut(s) 51, 173
Bsp1286I GDGCHC 1 cut(s) 357
Bsp143I GATC 3 cut(s) 98, 189, 502
BspACI CCGC 1 cut(s) 60
BspANI GGCC 2 cut(s) 51, 173
BspPI GGATC 2 cut(s) 106, 510
BsrDI GCAATG 1 cut(s) 400
BsrI ACTGG 2 cut(s) 476, 598
BssECI CCNNGG 3 cut(s) 108, 168, 406
BssMI GATC 3 cut(s) 98, 189, 502
BssNAI GTATAC 1 cut(s) 273
Bst1107I GTATAC 1 cut(s) 273
Bst2UI CCWGG 2 cut(s) 408, 483
Bst4CI ACNGT 2 cut(s) 82, 542
BstC8I GCNNGC 3 cut(s) 49, 304, 316
BstDEI CTNAG 1 cut(s) 666
BstDSI CCRYGG 1 cut(s) 168
BstH2I RGCGCY 2 cut(s) 108, 303
BstHHI GCGC 3 cut(s) 107, 206, 302
BstKTI GATC 3 cut(s) 101, 192, 505
BstMAI GTCTC 2 cut(s) 9, 123
BstMBI GATC 3 cut(s) 98, 189, 502
BstMWI GCNNNNNNNGC 4 cut(s) 65, 324, 379, 458
BstNI CCWGG 2 cut(s) 408, 483
BstSCI CCNGG 3 cut(s) 107, 406, 481
BstSFI CTRYAG 1 cut(s) 145
BstV1I GCAGC 2 cut(s) 218, 221
BstV2I GAAGAC 1 cut(s) 596
BstX2I RGATCY 1 cut(s) 189
BstYI RGATCY 1 cut(s) 189
BstZ17I GTATAC 1 cut(s) 273
BsuI GTATCC 1 cut(s) 238
BsuRI GGCC 2 cut(s) 51, 173
BtgI CCRYGG 1 cut(s) 168
BtrI CACGTC 1 cut(s) 331
BtsI GCAGTG 1 cut(s) 389
BtsIMutI CAGTG 3 cut(s) 78, 389, 469
Cac8I GCNNGC 3 cut(s) 49, 304, 316
CfoI GCGC 3 cut(s) 107, 206, 302
CseI GACGC 1 cut(s) 379
CviAII CATG 3 cut(s) 56, 65, 293
DdeI CTNAG 1 cut(s) 666
DpnI GATC 3 cut(s) 100, 191, 504
DpnII GATC 3 cut(s) 98, 189, 502
DrdI GACNNNNNNGTC 1 cut(s) 80
DseDI GACNNNNNNGTC 1 cut(s) 80
EaeI YGGCCR 1 cut(s) 171
Eco147I AGGCCT 1 cut(s) 51
EcoRII CCWGG 2 cut(s) 406, 481
FaeI CATG 3 cut(s) 59, 68, 296
FatI CATG 3 cut(s) 55, 64, 292
FauNDI CATATG 2 cut(s) 182, 377
FblI GTMKAC 2 cut(s) 72, 272
Fnu4HI GCNGC 3 cut(s) 60, 207, 210
Fsp4HI GCNGC 3 cut(s) 60, 207, 210
FspBI CTAG 2 cut(s) 276, 525
GlaI GCGC 3 cut(s) 106, 205, 301
GluI GCNGC 3 cut(s) 60, 207, 210
GsaI CCCAGC 3 cut(s) 199, 456, 561
HaeII RGCGCY 2 cut(s) 108, 303
HaeIII GGCC 2 cut(s) 51, 173
HapII CCGG 3 cut(s) 96, 108, 324
HgaI GACGC 1 cut(s) 379
HhaI GCGC 3 cut(s) 107, 206, 302
Hin1II CATG 3 cut(s) 59, 68, 296
Hin6I GCGC 3 cut(s) 105, 204, 300
HinP1I GCGC 3 cut(s) 105, 204, 300
HincII GTYRAC 2 cut(s) 73, 607
HindII GTYRAC 2 cut(s) 73, 607
HinfI GANTC 3 cut(s) 289, 365, 466
HpaI GTTAAC 1 cut(s) 607
HpaII CCGG 3 cut(s) 96, 108, 324
Hpy166II GTNNAC 3 cut(s) 73, 273, 607
Hpy188I TCNGA 2 cut(s) 119, 137
Hpy8I GTNNAC 3 cut(s) 73, 273, 607
Hpy99I CGWCG 2 cut(s) 332, 373
HpyAV CCTTC 4 cut(s) 41, 62, 416, 442
HpyCH4III ACNGT 2 cut(s) 82, 542
HpyCH4IV ACGT 2 cut(s) 330, 636
HpyCH4V TGCA 5 cut(s) 47, 68, 431, 461, 569
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 324, 379, 458
HpyF3I CTNAG 1 cut(s) 666
HpySE526I ACGT 2 cut(s) 330, 636
Hsp92II CATG 3 cut(s) 59, 68, 296
HspAI GCGC 3 cut(s) 105, 204, 300
KspAI GTTAAC 1 cut(s) 607
Kzo9I GATC 3 cut(s) 98, 189, 502
LmnI GCTCC 1 cut(s) 137
Lsp1109I GCAGC 2 cut(s) 218, 221
MaeI CTAG 2 cut(s) 276, 525
MaeII ACGT 2 cut(s) 330, 636
MaeIII GTNAC 3 cut(s) 82, 467, 517
MalI GATC 3 cut(s) 100, 191, 504
MboI GATC 3 cut(s) 98, 189, 502
MboII GAAGA 2 cut(s) 199, 601
MflI RGATCY 1 cut(s) 189
MhlI GDGCHC 1 cut(s) 357
MluCI AATT 2 cut(s) 175, 426
MlyI GAGTC 1 cut(s) 475
MnlI CCTC 2 cut(s) 35, 435
MseI TTAA 4 cut(s) 123, 477, 606, 639
MspI CCGG 3 cut(s) 96, 108, 324
MspR9I CCNGG 3 cut(s) 109, 408, 483
MvaI CCWGG 2 cut(s) 408, 483
MwoI GCNNNNNNNGC 4 cut(s) 65, 324, 379, 458
NciI CCSGG 1 cut(s) 109
NdeI CATATG 2 cut(s) 182, 377
NdeII GATC 3 cut(s) 98, 189, 502
NlaIII CATG 3 cut(s) 59, 68, 296
NmuCI GTSAC 2 cut(s) 82, 467
PceI AGGCCT 1 cut(s) 51
PfeI GAWTC 2 cut(s) 289, 365
PkrI GCNGC 3 cut(s) 61, 208, 211
PleI GAGTC 1 cut(s) 474
PpsI GAGTC 1 cut(s) 474
Psp1406I AACGTT 1 cut(s) 636
Psp6I CCWGG 2 cut(s) 406, 481
PspFI CCCAGC 3 cut(s) 195, 452, 557
PspGI CCWGG 2 cut(s) 406, 481
PsuI RGATCY 1 cut(s) 189
SalI GTCGAC 1 cut(s) 71
SaqAI TTAA 4 cut(s) 123, 477, 606, 639
SatI GCNGC 3 cut(s) 60, 207, 210
Sau3AI GATC 3 cut(s) 98, 189, 502
SchI GAGTC 1 cut(s) 475
ScrFI CCNGG 3 cut(s) 109, 408, 483
SduI GDGCHC 1 cut(s) 357
SfcI CTRYAG 1 cut(s) 145
Sse9I AATT 2 cut(s) 175, 426
SseBI AGGCCT 1 cut(s) 51
SsiI CCGC 1 cut(s) 60
SspMI CTAG 2 cut(s) 276, 525
StuI AGGCCT 1 cut(s) 51
StyD4I CCNGG 3 cut(s) 107, 406, 481
TaaI ACNGT 2 cut(s) 82, 542
TaiI ACGT 2 cut(s) 333, 639
TaqI TCGA 2 cut(s) 72, 368
TasI AATT 2 cut(s) 175, 426
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 2 cut(s) 289, 365
Tru1I TTAA 4 cut(s) 123, 477, 606, 639
Tru9I TTAA 4 cut(s) 123, 477, 606, 639
TscAI CASTG 3 cut(s) 85, 389, 476
TseFI GTSAC 2 cut(s) 82, 467
TseI GCWGC 2 cut(s) 206, 209
Tsp45I GTSAC 2 cut(s) 82, 467
TspDTI ATGAA 3 cut(s) 44, 169, 281
TspRI CASTG 3 cut(s) 85, 389, 476
XmiI GTMKAC 2 cut(s) 72, 272
XspI CTAG 2 cut(s) 276, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.