Rroxscaffold_7G00196500

Transmembrane protein 18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0015433
Physical Location & Seq
Forward (+)
39510817 .. 39513730
2914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00196500.1

Sequence Viewer

Length: 450 bp
ATGGAGCAGATTTGGTCTGCTATGGAAGCTCACCTGGACCAAGTCGCAGATCTCGTCCAGAAACTCTCCGCAGACTTCCGCTCTGGTCTTGGACCCGCTTTAGATAACTTCATTGGTTTTTTCCACGCTATCGACTGGAAGGAACCATGGTTGATGGGTTTAGTGGGACTCCACTTTCTGTTGTTGGTAATAGCCATTCTCTCCAGGAAGAATCTTAACTTCCAAATGCTTTTGTTCCTTACGGCCTTGGCTGGAGTTTATCTTGCTGAGAGGTTCAATGGTTTCCTGGGTCAGAACTGGAAGAGTTTCGCCGGCCAGAATTACTTTGATTCAAATGGAGTTTTTTTGTCAGTACTTTGGTCTGGGCCTCTTTCTCGTAATTGCAATTATAATTTTGATAAACACGCTCTTTTCCTTGTGTCACCTGATTGTCAAGTGGAAAAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM18

Protein Analysis

149

Amino Acids

16.88

Weight (kDa)

5.71

Isoelectric Point (pI)

16.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM18 PF14770 39 - 127 4.2e-34 Transmembrane protein 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015433)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34350 AT1G34350
fragaria_vesca FvH4_2g12231
malus_domestica MD05G1040400.v1.1 MD10G1046500.v1.1
prunus_persica Prupe.8G054100_v2.0.a1
pyrus_communis pycom05g03300 pycom10g03270
rosa_chinensis RchiOBHm_Chr6g0272201
rosa_laevigata RLG00000013692
rosa_multiflora Rmu_sc0005748.1_g000009
rosa_roxburghii Rroxscaffold_7G00196500
rosa_rugosa Rorug06G0067100
rosa_samantha Rh6AG183600
rosa_wichuraiana Rw0G003850 Rw6G015830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 390
AccBSI CCGCTC 1 cut(s) 81
AciI CCGC 3 cut(s) 69, 79, 96
AcoI YGGCCR 1 cut(s) 313
AfaI GTAC 1 cut(s) 354
AgsI TTSAA 2 cut(s) 277, 333
AjnI CCWGG 3 cut(s) 33, 203, 285
AluBI AGCT 2 cut(s) 29, 447
AluI AGCT 2 cut(s) 29, 447
AoxI GGCC 3 cut(s) 243, 313, 365
Asp700I GAANNNNTTC 1 cut(s) 305
AspS9I GGNCC 3 cut(s) 37, 92, 365
AsuHPI GGTGA 2 cut(s) 23, 414
AvaII GGWCC 2 cut(s) 37, 92
BccI CCATC 1 cut(s) 148
BceAI ACGGC 1 cut(s) 258
BciT130I CCWGG 3 cut(s) 35, 205, 287
BglII AGATCT 1 cut(s) 49
BmcAI AGTACT 1 cut(s) 354
Bme1390I CCNGG 3 cut(s) 35, 205, 287
Bme18I GGWCC 2 cut(s) 37, 92
BmgT120I GGNCC 3 cut(s) 37, 92, 365
BmiI GGNNCC 2 cut(s) 94, 144
BmrFI CCNGG 3 cut(s) 35, 205, 287
BpmI CTGGAG 2 cut(s) 187, 273
BsaJI CCNNGG 3 cut(s) 146, 246, 286
Bse118I RCCGGY 1 cut(s) 311
Bse1I ACTGG 2 cut(s) 140, 302
BseBI CCWGG 3 cut(s) 35, 205, 287
BseDI CCNNGG 3 cut(s) 146, 246, 286
BseMII CTCAG 1 cut(s) 258
BseNI ACTGG 2 cut(s) 140, 302
BshFI GGCC 3 cut(s) 245, 315, 367
BsiSI CCGG 1 cut(s) 312
BslFI GGGAC 1 cut(s) 180
BsmFI GGGAC 1 cut(s) 180
BsnI GGCC 3 cut(s) 245, 315, 367
Bsp143I GATC 1 cut(s) 49
Bsp19I CCATGG 1 cut(s) 146
BspACI CCGC 3 cut(s) 69, 79, 96
BspANI GGCC 3 cut(s) 245, 315, 367
BspCNI CTCAG 1 cut(s) 259
BspLI GGNNCC 2 cut(s) 94, 144
BsrBI CCGCTC 1 cut(s) 81
BsrFI RCCGGY 1 cut(s) 311
BsrI ACTGG 2 cut(s) 140, 302
BssAI RCCGGY 1 cut(s) 311
BssECI CCNNGG 3 cut(s) 146, 246, 286
BssMI GATC 1 cut(s) 49
BssT1I CCWWGG 2 cut(s) 146, 246
Bst2UI CCWGG 3 cut(s) 35, 205, 287
Bst6I CTCTTC 1 cut(s) 296
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 1 cut(s) 267
BstDSI CCRYGG 1 cut(s) 146
BstKTI GATC 1 cut(s) 52
BstMBI GATC 1 cut(s) 49
BstMWI GCNNNNNNNGC 1 cut(s) 26
BstNI CCWGG 3 cut(s) 35, 205, 287
BstSCI CCNGG 3 cut(s) 33, 203, 285
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BsuRI GGCC 3 cut(s) 245, 315, 367
BtgI CCRYGG 1 cut(s) 146
Cac8I GCNNGC 1 cut(s) 313
Cfr10I RCCGGY 1 cut(s) 311
Cfr13I GGNCC 3 cut(s) 37, 92, 365
Csp6I GTAC 1 cut(s) 353
CviAII CATG 1 cut(s) 147
CviJI RGCY 7 cut(s) 29, 194, 245, 251, 315, 367, 447
CviKI_1 RGCY 7 cut(s) 29, 194, 245, 251, 315, 367, 447
CviQI GTAC 1 cut(s) 353
DdeI CTNAG 1 cut(s) 267
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
EaeI YGGCCR 1 cut(s) 313
Eam1104I CTCTTC 1 cut(s) 296
EarI CTCTTC 1 cut(s) 296
Eco130I CCWWGG 2 cut(s) 146, 246
Eco47I GGWCC 2 cut(s) 37, 92
EcoRII CCWGG 3 cut(s) 33, 203, 285
EcoT14I CCWWGG 2 cut(s) 146, 246
ErhI CCWWGG 2 cut(s) 146, 246
FaeI CATG 1 cut(s) 150
FaiI YATR 3 cut(s) 23, 148, 390
FaqI GGGAC 1 cut(s) 180
FatI CATG 1 cut(s) 146
FauI CCCGC 1 cut(s) 103
GsuI CTGGAG 2 cut(s) 187, 273
HaeIII GGCC 3 cut(s) 245, 315, 367
HapII CCGG 1 cut(s) 312
Hin1II CATG 1 cut(s) 150
HinfI GANTC 3 cut(s) 168, 211, 329
HpaII CCGG 1 cut(s) 312
HphI GGTGA 2 cut(s) 23, 414
Hpy188I TCNGA 1 cut(s) 294
Hpy188III TCNNGA 1 cut(s) 58
HpyAV CCTTC 1 cut(s) 133
HpyCH4V TGCA 1 cut(s) 384
HpyF10VI GCNNNNNNNGC 1 cut(s) 26
HpyF3I CTNAG 1 cut(s) 267
Hsp92II CATG 1 cut(s) 150
KroI GCCGGC 1 cut(s) 311
KroNI GCCGGC 1 cut(s) 313
Kzo9I GATC 1 cut(s) 49
LmnI GCTCC 1 cut(s) 4
MaeIII GTNAC 1 cut(s) 420
MalI GATC 1 cut(s) 51
MbiI CCGCTC 1 cut(s) 81
MboI GATC 1 cut(s) 49
MboII GAAGA 2 cut(s) 220, 313
MflI RGATCY 1 cut(s) 49
MluCI AATT 4 cut(s) 319, 379, 385, 391
MlyI GAGTC 1 cut(s) 162
MnlI CCTC 2 cut(s) 264, 378
MroNI GCCGGC 1 cut(s) 311
MroXI GAANNNNTTC 1 cut(s) 305
MseI TTAA 1 cut(s) 216
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 3 cut(s) 35, 205, 287
MvaI CCWGG 3 cut(s) 35, 205, 287
MwoI GCNNNNNNNGC 1 cut(s) 26
NaeI GCCGGC 1 cut(s) 313
NcoI CCATGG 1 cut(s) 146
NdeII GATC 1 cut(s) 49
NgoMIV GCCGGC 1 cut(s) 311
NlaIII CATG 1 cut(s) 150
NlaIV GGNNCC 2 cut(s) 94, 144
NmuCI GTSAC 1 cut(s) 420
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PdiI GCCGGC 1 cut(s) 313
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 2 cut(s) 211, 329
PflFI GACNNNGTC 1 cut(s) 41
PfoI TCCNGGA 1 cut(s) 203
PleI GAGTC 1 cut(s) 162
PpsI GAGTC 1 cut(s) 162
PsiI TTATAA 1 cut(s) 390
Psp6I CCWGG 3 cut(s) 33, 203, 285
PspGI CCWGG 3 cut(s) 33, 203, 285
PspN4I GGNNCC 2 cut(s) 94, 144
PspPI GGNCC 3 cut(s) 37, 92, 365
PsuI RGATCY 1 cut(s) 49
PsyI GACNNNGTC 1 cut(s) 41
RsaI GTAC 1 cut(s) 354
RsaNI GTAC 1 cut(s) 353
SaqAI TTAA 1 cut(s) 216
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 3 cut(s) 37, 92, 365
ScaI AGTACT 1 cut(s) 354
SchI GAGTC 1 cut(s) 162
ScrFI CCNGG 3 cut(s) 35, 205, 287
SetI ASST 5 cut(s) 31, 36, 275, 427, 449
SinI GGWCC 2 cut(s) 37, 92
Sse9I AATT 4 cut(s) 319, 379, 385, 391
SsiI CCGC 3 cut(s) 69, 79, 96
StyD4I CCNGG 3 cut(s) 33, 203, 285
StyI CCWWGG 2 cut(s) 146, 246
TaqI TCGA 1 cut(s) 132
TasI AATT 4 cut(s) 319, 379, 385, 391
TatI WGTACW 1 cut(s) 352
TfiI GAWTC 2 cut(s) 211, 329
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TseFI GTSAC 1 cut(s) 420
Tsp45I GTSAC 1 cut(s) 420
TspDTI ATGAA 1 cut(s) 100
Tth111I GACNNNGTC 1 cut(s) 41
VpaK11BI GGWCC 2 cut(s) 37, 92
XmnI GAANNNNTTC 1 cut(s) 305
ZrmI AGTACT 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.