Rw0G003850

Transmembrane protein 18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00144
OG0015433
Physical Location & Seq
Forward (+)
40894 .. 43861
2968 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G003850.1

Sequence Viewer

Length: 492 bp
ATGGAGCAGATTCGGTCTGCCATGGAAGCTCACCTGGACCAAGTAGCAGATCTCGTCCAGAAACTGTCCGCGGACTTCCGCTCTGGTCTTGGACCCGCTTTAGATAACTTCATTGGCTTTTTCCACGCTATCGATTGGAAGGAACCATGGTTGATGGGTTTAGTGGGACTCCACTTTCTGTTGTTGATAATAGCCATTCTCTCCAGGAAGAATCTTAACTTCCAAATGCTTTTGTTCCTTACGGCCTTGGCTGGAGTTTATCTTGCTGAGAGGTTCAATGGTTTCCTGGGTCAGAACTGGAAGGGTTTCGCCGGCCAGAATTACTTTGATTCAAATGGAGTTTTTTTGTCAGTACTCTGGTCTGGGCCTCTTCTCGTAATTGCAATTATAATTTTGATAAACACGCTCTTTTCCTTATGTCACCTGATTGTCAAGTGGAAAAGAGCTGAGCTGAGACACCGTGCACGGCTTTCTCAGAATAAGCAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM18

Protein Analysis

163

Amino Acids

18.54

Weight (kDa)

9.25

Isoelectric Point (pI)

27.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM18 PF14770 39 - 156 1.4e-45 Transmembrane protein 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015433)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34350 AT1G34350
fragaria_vesca FvH4_2g12231
malus_domestica MD05G1040400.v1.1 MD10G1046500.v1.1
prunus_persica Prupe.8G054100_v2.0.a1
pyrus_communis pycom05g03300 pycom10g03270
rosa_chinensis RchiOBHm_Chr6g0272201
rosa_laevigata RLG00000013692
rosa_multiflora Rmu_sc0005748.1_g000009
rosa_roxburghii Rroxscaffold_7G00196500
rosa_rugosa Rorug06G0067100
rosa_samantha Rh6AG183600
rosa_wichuraiana Rw0G003850 Rw6G015830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 389
AccBSI CCGCTC 1 cut(s) 81
AccII CGCG 1 cut(s) 71
AciI CCGC 4 cut(s) 69, 71, 79, 96
AcoI YGGCCR 1 cut(s) 313
AfaI GTAC 1 cut(s) 354
AgsI TTSAA 2 cut(s) 277, 333
AjnI CCWGG 3 cut(s) 33, 203, 285
AluBI AGCT 3 cut(s) 29, 446, 451
AluI AGCT 3 cut(s) 29, 446, 451
Alw21I GWGCWC 1 cut(s) 466
Alw26I GTCTC 1 cut(s) 448
Alw44I GTGCAC 1 cut(s) 462
AlwNI CAGNNNCTG 1 cut(s) 64
AoxI GGCC 3 cut(s) 243, 313, 365
ApaLI GTGCAC 1 cut(s) 462
Asp700I GAANNNNTTC 1 cut(s) 305
AspS9I GGNCC 3 cut(s) 37, 92, 365
AsuHPI GGTGA 2 cut(s) 23, 413
AvaII GGWCC 2 cut(s) 37, 92
BaeGI GKGCMC 1 cut(s) 466
Bbv12I GWGCWC 1 cut(s) 466
BccI CCATC 1 cut(s) 148
BceAI ACGGC 2 cut(s) 258, 482
BciT130I CCWGG 3 cut(s) 35, 205, 287
BcoDI GTCTC 1 cut(s) 448
BglII AGATCT 1 cut(s) 49
BlpI GCTNAGC 1 cut(s) 447
BmcAI AGTACT 1 cut(s) 354
Bme1390I CCNGG 3 cut(s) 35, 205, 287
Bme18I GGWCC 2 cut(s) 37, 92
BmgT120I GGNCC 3 cut(s) 37, 92, 365
BmiI GGNNCC 2 cut(s) 94, 144
BmrFI CCNGG 3 cut(s) 35, 205, 287
BpmI CTGGAG 2 cut(s) 187, 273
Bpu1102I GCTNAGC 1 cut(s) 447
Bsa29I ATCGAT 1 cut(s) 132
BsaJI CCNNGG 5 cut(s) 21, 69, 146, 246, 286
Bse118I RCCGGY 1 cut(s) 311
Bse1I ACTGG 1 cut(s) 302
BseBI CCWGG 3 cut(s) 35, 205, 287
BseCI ATCGAT 1 cut(s) 132
BseDI CCNNGG 5 cut(s) 21, 69, 146, 246, 286
BseMII CTCAG 4 cut(s) 258, 438, 443, 488
BseNI ACTGG 1 cut(s) 302
BseSI GKGCMC 1 cut(s) 466
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 3 cut(s) 245, 315, 367
BshVI ATCGAT 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 466
BsiSI CCGG 1 cut(s) 312
BslFI GGGAC 1 cut(s) 180
BsmAI GTCTC 1 cut(s) 448
BsmFI GGGAC 1 cut(s) 180
BsnI GGCC 3 cut(s) 245, 315, 367
Bsp1286I GDGCHC 1 cut(s) 466
Bsp143I GATC 1 cut(s) 49
Bsp1720I GCTNAGC 1 cut(s) 447
Bsp19I CCATGG 2 cut(s) 21, 146
BspACI CCGC 4 cut(s) 69, 71, 79, 96
BspANI GGCC 3 cut(s) 245, 315, 367
BspCNI CTCAG 4 cut(s) 259, 439, 444, 487
BspDI ATCGAT 1 cut(s) 132
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 2 cut(s) 94, 144
BsrBI CCGCTC 1 cut(s) 81
BsrFI RCCGGY 1 cut(s) 311
BsrI ACTGG 1 cut(s) 302
BssAI RCCGGY 1 cut(s) 311
BssECI CCNNGG 5 cut(s) 21, 69, 146, 246, 286
BssMI GATC 1 cut(s) 49
BssT1I CCWWGG 3 cut(s) 21, 146, 246
Bst2UI CCWGG 3 cut(s) 35, 205, 287
Bst4CI ACNGT 2 cut(s) 66, 461
Bst6I CTCTTC 1 cut(s) 375
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 4 cut(s) 267, 447, 452, 474
BstDSI CCRYGG 3 cut(s) 21, 69, 146
BstFNI CGCG 1 cut(s) 71
BstKTI GATC 1 cut(s) 52
BstMAI GTCTC 1 cut(s) 448
BstMBI GATC 1 cut(s) 49
BstMWI GCNNNNNNNGC 1 cut(s) 26
BstNI CCWGG 3 cut(s) 35, 205, 287
BstSCI CCNGG 3 cut(s) 33, 203, 285
BstSLI GKGCMC 1 cut(s) 466
BstUI CGCG 1 cut(s) 71
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
Bsu15I ATCGAT 1 cut(s) 132
BsuRI GGCC 3 cut(s) 245, 315, 367
BsuTUI ATCGAT 1 cut(s) 132
BtgI CCRYGG 3 cut(s) 21, 69, 146
Cac8I GCNNGC 1 cut(s) 313
CaiI CAGNNNCTG 1 cut(s) 64
Cfr10I RCCGGY 1 cut(s) 311
Cfr13I GGNCC 3 cut(s) 37, 92, 365
Cfr42I CCGCGG 1 cut(s) 72
ClaI ATCGAT 1 cut(s) 132
Csp6I GTAC 1 cut(s) 353
CviAII CATG 2 cut(s) 22, 147
CviQI GTAC 1 cut(s) 353
DdeI CTNAG 4 cut(s) 267, 447, 452, 474
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
EaeI YGGCCR 1 cut(s) 313
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Eco130I CCWWGG 3 cut(s) 21, 146, 246
Eco47I GGWCC 2 cut(s) 37, 92
EcoRII CCWGG 3 cut(s) 33, 203, 285
EcoT14I CCWWGG 3 cut(s) 21, 146, 246
ErhI CCWWGG 3 cut(s) 21, 146, 246
FaeI CATG 2 cut(s) 25, 150
FaiI YATR 4 cut(s) 23, 148, 389, 418
FaqI GGGAC 1 cut(s) 180
FatI CATG 2 cut(s) 21, 146
FauI CCCGC 1 cut(s) 103
GsuI CTGGAG 2 cut(s) 187, 273
HaeIII GGCC 3 cut(s) 245, 315, 367
HapII CCGG 1 cut(s) 312
Hin1II CATG 2 cut(s) 25, 150
HinfI GANTC 4 cut(s) 10, 168, 211, 329
HpaII CCGG 1 cut(s) 312
HphI GGTGA 2 cut(s) 23, 413
Hpy166II GTNNAC 1 cut(s) 464
Hpy188I TCNGA 2 cut(s) 294, 477
Hpy188III TCNNGA 1 cut(s) 58
Hpy8I GTNNAC 1 cut(s) 464
HpyAV CCTTC 2 cut(s) 133, 295
HpyCH4III ACNGT 2 cut(s) 66, 461
HpyCH4V TGCA 2 cut(s) 383, 464
HpyF10VI GCNNNNNNNGC 1 cut(s) 26
HpyF3I CTNAG 4 cut(s) 267, 447, 452, 474
Hsp92II CATG 2 cut(s) 25, 150
KroI GCCGGC 1 cut(s) 311
KroNI GCCGGC 1 cut(s) 313
KspI CCGCGG 1 cut(s) 72
Kzo9I GATC 1 cut(s) 49
LmnI GCTCC 1 cut(s) 4
MaeIII GTNAC 1 cut(s) 419
MalI GATC 1 cut(s) 51
MbiI CCGCTC 1 cut(s) 81
MboI GATC 1 cut(s) 49
MboII GAAGA 2 cut(s) 220, 362
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 1 cut(s) 466
MluCI AATT 4 cut(s) 319, 378, 384, 390
MlyI GAGTC 1 cut(s) 162
MnlI CCTC 2 cut(s) 264, 378
MroNI GCCGGC 1 cut(s) 311
MroXI GAANNNNTTC 1 cut(s) 305
MseI TTAA 1 cut(s) 216
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 3 cut(s) 35, 205, 287
MvaI CCWGG 3 cut(s) 35, 205, 287
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 1 cut(s) 26
NaeI GCCGGC 1 cut(s) 313
NcoI CCATGG 2 cut(s) 21, 146
NdeII GATC 1 cut(s) 49
NgoMIV GCCGGC 1 cut(s) 311
NlaIII CATG 2 cut(s) 25, 150
NlaIV GGNNCC 2 cut(s) 94, 144
NmuCI GTSAC 1 cut(s) 419
PdiI GCCGGC 1 cut(s) 313
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 3 cut(s) 10, 211, 329
PfoI TCCNGGA 1 cut(s) 203
PleI GAGTC 1 cut(s) 162
PpsI GAGTC 1 cut(s) 162
PsiI TTATAA 1 cut(s) 389
Psp6I CCWGG 3 cut(s) 33, 203, 285
PspGI CCWGG 3 cut(s) 33, 203, 285
PspN4I GGNNCC 2 cut(s) 94, 144
PspPI GGNCC 3 cut(s) 37, 92, 365
PstNI CAGNNNCTG 1 cut(s) 64
PsuI RGATCY 1 cut(s) 49
RsaI GTAC 1 cut(s) 354
RsaNI GTAC 1 cut(s) 353
SacII CCGCGG 1 cut(s) 72
SaqAI TTAA 1 cut(s) 216
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 3 cut(s) 37, 92, 365
ScaI AGTACT 1 cut(s) 354
SchI GAGTC 1 cut(s) 162
ScrFI CCNGG 3 cut(s) 35, 205, 287
SduI GDGCHC 1 cut(s) 466
SetI ASST 6 cut(s) 31, 36, 275, 426, 448, 453
Sfr303I CCGCGG 1 cut(s) 72
SgrBI CCGCGG 1 cut(s) 72
SinI GGWCC 2 cut(s) 37, 92
Sse9I AATT 4 cut(s) 319, 378, 384, 390
SsiI CCGC 4 cut(s) 69, 71, 79, 96
StyD4I CCNGG 3 cut(s) 33, 203, 285
StyI CCWWGG 3 cut(s) 21, 146, 246
TaaI ACNGT 2 cut(s) 66, 461
TaqI TCGA 1 cut(s) 132
TasI AATT 4 cut(s) 319, 378, 384, 390
TatI WGTACW 1 cut(s) 352
TfiI GAWTC 3 cut(s) 10, 211, 329
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TseFI GTSAC 1 cut(s) 419
Tsp45I GTSAC 1 cut(s) 419
TspDTI ATGAA 1 cut(s) 100
VneI GTGCAC 1 cut(s) 462
VpaK11BI GGWCC 2 cut(s) 37, 92
XmnI GAANNNNTTC 1 cut(s) 305
ZrmI AGTACT 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.