pycom05g03300

Transmembrane protein 18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0015433
Physical Location & Seq
Forward (+)
3825494 .. 3827668
2175 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g03300.2

Sequence Viewer

Length: 492 bp
ATGGAGCAGGTTCGGTCGGCAGTGGAGGAGCACCTGGATCAAATGGCAGATCTCGTCCAGAAAATATCCGCAGAGTTACGTTCTGGTCTTGCACCTGCTGTAAACACTTTCTTGGGCTTTTTCCACGCCATCGACTGGAAGGAACCTTGGTTGATGGGTTTAATGGGGATGCATCTTGTGTTTCTGATAGTAGCCATTACCTCCAGGAGGAATCTCAACTTCCAAATGTTTCTGTTCCTTTCGGCATTGGCTGGAGTTTATCTTGCAGAGAGGCTGAATAGTTTCCTGCGTGCGAACTGGAAGAGCTTTGCCAGTCAGGATTATTTTGATGAGGGTGGAGTTTTTCTCTCAACACTCTGGTCTGGGCCGCTTCTCGTCATTGCAATCATAATTTTGGTAAACACACTCTTTTCCTTATGTCGCCTGATTGTTAAGTGGAAAAGAGCTGAGCTAAGACATCGTGCTAGGCTTTCTCAAAGCAAGCAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM18

Protein Analysis

164

Amino Acids

18.67

Weight (kDa)

9.3

Isoelectric Point (pI)

46.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015433)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34350 AT1G34350
fragaria_vesca FvH4_2g12231
malus_domestica MD05G1040400.v1.1 MD10G1046500.v1.1
prunus_persica Prupe.8G054100_v2.0.a1
pyrus_communis pycom05g03300 pycom10g03270
rosa_chinensis RchiOBHm_Chr6g0272201
rosa_laevigata RLG00000013692
rosa_multiflora Rmu_sc0005748.1_g000009
rosa_roxburghii Rroxscaffold_7G00196500
rosa_rugosa Rorug06G0067100
rosa_samantha Rh6AG183600
rosa_wichuraiana Rw0G003850 Rw6G015830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 103
AccB7I CCANNNNNTGG 1 cut(s) 135
AciI CCGC 2 cut(s) 69, 368
AclWI GGATC 1 cut(s) 45
AfiI CCNNNNNNNGG 2 cut(s) 135, 207
AjnI CCWGG 2 cut(s) 33, 203
AluBI AGCT 3 cut(s) 306, 446, 451
AluI AGCT 3 cut(s) 306, 446, 451
Alw21I GWGCWC 1 cut(s) 33
AlwI GGATC 1 cut(s) 45
AoxI GGCC 1 cut(s) 365
Asp700I GAANNNNTTC 1 cut(s) 281
AspS9I GGNCC 1 cut(s) 365
Bbv12I GWGCWC 1 cut(s) 33
BccI CCATC 2 cut(s) 137, 148
BciT130I CCWGG 2 cut(s) 35, 205
BfaI CTAG 1 cut(s) 465
BfuAI ACCTGC 1 cut(s) 103
BglII AGATCT 1 cut(s) 49
BisI GCNGC 1 cut(s) 368
BlpI GCTNAGC 1 cut(s) 447
BlsI GCNGC 1 cut(s) 369
Bme1390I CCNGG 2 cut(s) 35, 205
BmgT120I GGNCC 1 cut(s) 365
BmiI GGNNCC 1 cut(s) 144
BmrFI CCNGG 2 cut(s) 35, 205
BmsI GCATC 2 cut(s) 159, 181
BplI GAGNNNNNCTC 2 cut(s) 330, 362
BpmI CTGGAG 2 cut(s) 187, 273
Bpu1102I GCTNAGC 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 146
Bsc4I CCNNNNNNNGG 2 cut(s) 135, 207
Bse1I ACTGG 3 cut(s) 140, 302, 312
Bse3DI GCAATG 1 cut(s) 378
BseBI CCWGG 2 cut(s) 35, 205
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 1 cut(s) 174
BseLI CCNNNNNNNGG 2 cut(s) 135, 207
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 1 cut(s) 438
BseNI ACTGG 3 cut(s) 140, 302, 312
BseRI GAGGAG 1 cut(s) 41
Bsh1285I CGRYCG 1 cut(s) 17
BshFI GGCC 1 cut(s) 367
BsiEI CGRYCG 1 cut(s) 17
BsiHKAI GWGCWC 1 cut(s) 33
BslI CCNNNNNNNGG 2 cut(s) 135, 207
BsnI GGCC 1 cut(s) 367
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 2 cut(s) 37, 49
Bsp1720I GCTNAGC 1 cut(s) 447
BspACI CCGC 2 cut(s) 69, 368
BspANI GGCC 1 cut(s) 367
BspCNI CTCAG 1 cut(s) 439
BspLI GGNNCC 1 cut(s) 144
BspMI ACCTGC 1 cut(s) 103
BspPI GGATC 1 cut(s) 45
BspQI GCTCTTC 1 cut(s) 296
BsrDI GCAATG 1 cut(s) 378
BsrI ACTGG 3 cut(s) 140, 302, 312
BssECI CCNNGG 1 cut(s) 146
BssMI GATC 2 cut(s) 37, 49
BssT1I CCWWGG 1 cut(s) 146
Bst2UI CCWGG 2 cut(s) 35, 205
Bst6I CTCTTC 1 cut(s) 296
BstC8I GCNNGC 2 cut(s) 291, 482
BstDEI CTNAG 2 cut(s) 447, 452
BstENI CCTNNNNNAGG 1 cut(s) 205
BstF5I GGATG 1 cut(s) 174
BstKTI GATC 2 cut(s) 40, 52
BstMBI GATC 2 cut(s) 37, 49
BstMCI CGRYCG 1 cut(s) 17
BstNI CCWGG 2 cut(s) 35, 205
BstSCI CCNGG 2 cut(s) 33, 203
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BsuRI GGCC 1 cut(s) 367
BtsCI GGATG 1 cut(s) 174
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 1 cut(s) 27
BveI ACCTGC 1 cut(s) 103
Cac8I GCNNGC 2 cut(s) 291, 482
Cfr13I GGNCC 1 cut(s) 365
CviJI RGCY 9 cut(s) 117, 194, 251, 274, 306, 367, 446, 451, 469
CviKI_1 RGCY 9 cut(s) 117, 194, 251, 274, 306, 367, 446, 451, 469
DdeI CTNAG 2 cut(s) 447, 452
DpnI GATC 2 cut(s) 39, 51
DpnII GATC 2 cut(s) 37, 49
Eam1104I CTCTTC 1 cut(s) 296
EarI CTCTTC 1 cut(s) 296
Eco130I CCWWGG 1 cut(s) 146
EcoNI CCTNNNNNAGG 1 cut(s) 205
EcoRII CCWGG 2 cut(s) 33, 203
EcoT14I CCWWGG 1 cut(s) 146
EcoT22I ATGCAT 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 146
FaiI YATR 2 cut(s) 389, 418
Fnu4HI GCNGC 1 cut(s) 368
FokI GGATG 1 cut(s) 181
Fsp4HI GCNGC 1 cut(s) 368
FspBI CTAG 1 cut(s) 465
GluI GCNGC 1 cut(s) 368
GsuI CTGGAG 2 cut(s) 187, 273
HaeIII GGCC 1 cut(s) 367
HinfI GANTC 1 cut(s) 211
Hpy166II GTNNAC 2 cut(s) 103, 400
Hpy188I TCNGA 1 cut(s) 186
Hpy188III TCNNGA 2 cut(s) 58, 317
Hpy8I GTNNAC 2 cut(s) 103, 400
HpyAV CCTTC 1 cut(s) 133
HpyCH4IV ACGT 1 cut(s) 79
HpyCH4V TGCA 4 cut(s) 92, 172, 266, 383
HpyF3I CTNAG 2 cut(s) 447, 452
HpySE526I ACGT 1 cut(s) 79
Kzo9I GATC 2 cut(s) 37, 49
LguI GCTCTTC 1 cut(s) 296
LmnI GCTCC 2 cut(s) 4, 28
LweI GCATC 2 cut(s) 159, 181
MaeI CTAG 1 cut(s) 465
MaeII ACGT 1 cut(s) 79
MaeIII GTNAC 1 cut(s) 75
MalI GATC 2 cut(s) 39, 51
MboI GATC 2 cut(s) 37, 49
MboII GAAGA 1 cut(s) 313
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 1 cut(s) 390
MnlI CCTC 5 cut(s) 19, 201, 211, 264, 325
Mph1103I ATGCAT 1 cut(s) 174
MroXI GAANNNNTTC 1 cut(s) 281
MseI TTAA 2 cut(s) 161, 432
MspR9I CCNGG 2 cut(s) 35, 205
MvaI CCWGG 2 cut(s) 35, 205
NdeII GATC 2 cut(s) 37, 49
NlaIV GGNNCC 1 cut(s) 144
NsiI ATGCAT 1 cut(s) 174
PaqCI CACCTGC 1 cut(s) 103
PciSI GCTCTTC 1 cut(s) 296
PdmI GAANNNNTTC 1 cut(s) 281
PfeI GAWTC 1 cut(s) 211
PflMI CCANNNNNTGG 1 cut(s) 135
PfoI TCCNGGA 1 cut(s) 203
PkrI GCNGC 1 cut(s) 369
Psp6I CCWGG 2 cut(s) 33, 203
PspGI CCWGG 2 cut(s) 33, 203
PspN4I GGNNCC 1 cut(s) 144
PspPI GGNCC 1 cut(s) 365
PsuI RGATCY 1 cut(s) 49
SapI GCTCTTC 1 cut(s) 296
SaqAI TTAA 2 cut(s) 161, 432
SatI GCNGC 1 cut(s) 368
Sau3AI GATC 2 cut(s) 37, 49
Sau96I GGNCC 1 cut(s) 365
ScrFI CCNGG 2 cut(s) 35, 205
SduI GDGCHC 1 cut(s) 33
SetI ASST 9 cut(s) 12, 36, 82, 97, 148, 203, 308, 448, 453
SfaNI GCATC 2 cut(s) 159, 181
Sse9I AATT 1 cut(s) 390
SsiI CCGC 2 cut(s) 69, 368
SspMI CTAG 1 cut(s) 465
StyD4I CCNGG 2 cut(s) 33, 203
StyI CCWWGG 1 cut(s) 146
TaiI ACGT 1 cut(s) 82
TaqI TCGA 1 cut(s) 132
TasI AATT 1 cut(s) 390
TauI GCSGC 1 cut(s) 370
TfiI GAWTC 1 cut(s) 211
Tru1I TTAA 2 cut(s) 161, 432
Tru9I TTAA 2 cut(s) 161, 432
TscAI CASTG 1 cut(s) 27
TspRI CASTG 1 cut(s) 27
Van91I CCANNNNNTGG 1 cut(s) 135
XagI CCTNNNNNAGG 1 cut(s) 205
XmnI GAANNNNTTC 1 cut(s) 281
XspI CTAG 1 cut(s) 465
Zsp2I ATGCAT 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.