MD10G1046500.v1.1

Transmembrane protein 18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015433
Physical Location & Seq
Forward (+)
6252396 .. 6255568
3173 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1046500.v1.1.491

Sequence Viewer

Length: 492 bp
ATGGAGCAGGTTCGGTCGGCAATGGAGGAGCACATGGATCAAATGGCAGATCTCGTCCAGAAGTTAACCGCAGAGTTACGTTCGGGTCTTGCACCTGCTGTAAACAATTTCTTGGGCTTTTTCCACGCTATCGACTGGACGGAACCTTGGTTGATGGGTTTAATGGGCATGCATCTTGTGCTTCTGATAGTAGCCATCACCTCCAGGAGGAATCTCAACTTCCAAATGTTTCTGTTCCTTTTGGCATTGGCAGGAGTATATCTTGCAGAGAGGCTCAATAGTTTCCTGCGTGCAAACTGGAAGAGCTTTGCCGGTCAGGATTATTTTGATGAGGGTGGAGTTTTTCTCTCAACGCTCTGGTCTGGGCCTCTTCTCGTCATTGCAATCATAATTTTGGTAAACACACTCTTTTCCTTGTGTCGCCTGATTGTTAAGTGGAAAAGAGCCGAGCTAAGACATCGTGCAAGGCTTTCTCAAAGTAAGCAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM18

Protein Analysis

164

Amino Acids

18.69

Weight (kDa)

8.93

Isoelectric Point (pI)

44.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM18 PF14770 39 - 156 1.2e-46 Transmembrane protein 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015433)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34350 AT1G34350
fragaria_vesca FvH4_2g12231
malus_domestica MD05G1040400.v1.1 MD10G1046500.v1.1
prunus_persica Prupe.8G054100_v2.0.a1
pyrus_communis pycom05g03300 pycom10g03270
rosa_chinensis RchiOBHm_Chr6g0272201
rosa_laevigata RLG00000013692
rosa_multiflora Rmu_sc0005748.1_g000009
rosa_roxburghii Rroxscaffold_7G00196500
rosa_rugosa Rorug06G0067100
rosa_samantha Rh6AG183600
rosa_wichuraiana Rw0G003850 Rw6G015830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 103
AciI CCGC 1 cut(s) 69
AclWI GGATC 1 cut(s) 45
AfiI CCNNNNNNNGG 1 cut(s) 207
AjnI CCWGG 1 cut(s) 203
AluBI AGCT 2 cut(s) 306, 451
AluI AGCT 2 cut(s) 306, 451
Alw21I GWGCWC 1 cut(s) 33
AlwI GGATC 1 cut(s) 45
AoxI GGCC 1 cut(s) 365
AspS9I GGNCC 1 cut(s) 365
AsuHPI GGTGA 1 cut(s) 190
Bbv12I GWGCWC 1 cut(s) 33
BccI CCATC 2 cut(s) 148, 203
BciT130I CCWGG 1 cut(s) 205
BfuAI ACCTGC 1 cut(s) 103
BglII AGATCT 1 cut(s) 49
Bme1390I CCNGG 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 365
BmiI GGNNCC 1 cut(s) 144
BmrFI CCNGG 1 cut(s) 205
BmsI GCATC 1 cut(s) 181
BplI GAGNNNNNCTC 2 cut(s) 330, 362
BpmI CTGGAG 1 cut(s) 187
BsaJI CCNNGG 1 cut(s) 146
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse118I RCCGGY 1 cut(s) 311
Bse1I ACTGG 2 cut(s) 140, 302
Bse3DI GCAATG 2 cut(s) 27, 378
BseBI CCWGG 1 cut(s) 205
BseDI CCNNGG 1 cut(s) 146
BseLI CCNNNNNNNGG 1 cut(s) 207
BseMI GCAATG 2 cut(s) 27, 378
BseNI ACTGG 2 cut(s) 140, 302
BseRI GAGGAG 1 cut(s) 41
Bsh1285I CGRYCG 1 cut(s) 17
BshFI GGCC 1 cut(s) 367
BsiEI CGRYCG 1 cut(s) 17
BsiHKAI GWGCWC 1 cut(s) 33
BsiSI CCGG 1 cut(s) 312
BslI CCNNNNNNNGG 1 cut(s) 207
BsnI GGCC 1 cut(s) 367
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 2 cut(s) 37, 49
BspACI CCGC 1 cut(s) 69
BspANI GGCC 1 cut(s) 367
BspLI GGNNCC 1 cut(s) 144
BspMI ACCTGC 1 cut(s) 103
BspPI GGATC 1 cut(s) 45
BspQI GCTCTTC 1 cut(s) 296
BsrDI GCAATG 2 cut(s) 27, 378
BsrFI RCCGGY 1 cut(s) 311
BsrI ACTGG 2 cut(s) 140, 302
BssAI RCCGGY 1 cut(s) 311
BssECI CCNNGG 1 cut(s) 146
BssMI GATC 2 cut(s) 37, 49
BssT1I CCWWGG 1 cut(s) 146
Bst2UI CCWGG 1 cut(s) 205
Bst6I CTCTTC 2 cut(s) 296, 375
BstAPI GCANNNNNTGC 1 cut(s) 178
BstC8I GCNNGC 2 cut(s) 170, 291
BstDEI CTNAG 1 cut(s) 452
BstENI CCTNNNNNAGG 1 cut(s) 205
BstKTI GATC 2 cut(s) 40, 52
BstMBI GATC 2 cut(s) 37, 49
BstMCI CGRYCG 1 cut(s) 17
BstMWI GCNNNNNNNGC 1 cut(s) 178
BstNI CCWGG 1 cut(s) 205
BstNSI RCATGY 1 cut(s) 172
BstSCI CCNGG 1 cut(s) 203
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BsuRI GGCC 1 cut(s) 367
BveI ACCTGC 1 cut(s) 103
Cac8I GCNNGC 2 cut(s) 170, 291
Cfr10I RCCGGY 1 cut(s) 311
Cfr13I GGNCC 1 cut(s) 365
CviAII CATG 2 cut(s) 34, 169
CviJI RGCY 8 cut(s) 117, 194, 274, 306, 367, 446, 451, 469
CviKI_1 RGCY 8 cut(s) 117, 194, 274, 306, 367, 446, 451, 469
DdeI CTNAG 1 cut(s) 452
DpnI GATC 2 cut(s) 39, 51
DpnII GATC 2 cut(s) 37, 49
Eam1104I CTCTTC 2 cut(s) 296, 375
EarI CTCTTC 2 cut(s) 296, 375
Eco130I CCWWGG 1 cut(s) 146
EcoNI CCTNNNNNAGG 1 cut(s) 205
EcoRII CCWGG 1 cut(s) 203
EcoT14I CCWWGG 1 cut(s) 146
EcoT22I ATGCAT 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 2 cut(s) 37, 172
FaiI YATR 4 cut(s) 35, 170, 259, 389
FatI CATG 2 cut(s) 33, 168
GsuI CTGGAG 1 cut(s) 187
HaeIII GGCC 1 cut(s) 367
HapII CCGG 1 cut(s) 312
Hin1II CATG 2 cut(s) 37, 172
HincII GTYRAC 1 cut(s) 66
HindII GTYRAC 1 cut(s) 66
HinfI GANTC 1 cut(s) 211
HpaI GTTAAC 1 cut(s) 66
HpaII CCGG 1 cut(s) 312
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 3 cut(s) 66, 103, 400
Hpy188I TCNGA 1 cut(s) 186
Hpy188III TCNNGA 2 cut(s) 58, 317
Hpy8I GTNNAC 3 cut(s) 66, 103, 400
HpyCH4IV ACGT 1 cut(s) 79
HpyCH4V TGCA 6 cut(s) 92, 172, 266, 293, 383, 464
HpyF10VI GCNNNNNNNGC 1 cut(s) 178
HpyF3I CTNAG 1 cut(s) 452
HpySE526I ACGT 1 cut(s) 79
Hsp92II CATG 2 cut(s) 37, 172
KspAI GTTAAC 1 cut(s) 66
Kzo9I GATC 2 cut(s) 37, 49
LguI GCTCTTC 1 cut(s) 296
LmnI GCTCC 2 cut(s) 4, 28
LweI GCATC 1 cut(s) 181
MaeII ACGT 1 cut(s) 79
MaeIII GTNAC 1 cut(s) 75
MalI GATC 2 cut(s) 39, 51
MboI GATC 2 cut(s) 37, 49
MboII GAAGA 2 cut(s) 313, 362
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 2 cut(s) 106, 390
MnlI CCTC 6 cut(s) 19, 201, 211, 264, 325, 378
Mph1103I ATGCAT 1 cut(s) 174
MseI TTAA 3 cut(s) 65, 161, 432
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 1 cut(s) 205
MvaI CCWGG 1 cut(s) 205
MwoI GCNNNNNNNGC 1 cut(s) 178
NdeII GATC 2 cut(s) 37, 49
NlaIII CATG 2 cut(s) 37, 172
NlaIV GGNNCC 1 cut(s) 144
NmeAIII GCCGAG 1 cut(s) 472
NsiI ATGCAT 1 cut(s) 174
NspI RCATGY 1 cut(s) 172
PaeI GCATGC 1 cut(s) 172
PaqCI CACCTGC 1 cut(s) 103
PciSI GCTCTTC 1 cut(s) 296
PfeI GAWTC 1 cut(s) 211
PfoI TCCNGGA 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 203
PspGI CCWGG 1 cut(s) 203
PspN4I GGNNCC 1 cut(s) 144
PspPI GGNCC 1 cut(s) 365
PsuI RGATCY 1 cut(s) 49
SapI GCTCTTC 1 cut(s) 296
SaqAI TTAA 3 cut(s) 65, 161, 432
Sau3AI GATC 2 cut(s) 37, 49
Sau96I GGNCC 1 cut(s) 365
ScrFI CCNGG 1 cut(s) 205
SduI GDGCHC 1 cut(s) 33
SetI ASST 7 cut(s) 12, 82, 97, 148, 203, 308, 453
SfaNI GCATC 1 cut(s) 181
SphI GCATGC 1 cut(s) 172
Sse9I AATT 2 cut(s) 106, 390
SsiI CCGC 1 cut(s) 69
StyD4I CCNGG 1 cut(s) 203
StyI CCWWGG 1 cut(s) 146
TaiI ACGT 1 cut(s) 82
TaqI TCGA 1 cut(s) 132
TasI AATT 2 cut(s) 106, 390
TfiI GAWTC 1 cut(s) 211
Tru1I TTAA 3 cut(s) 65, 161, 432
Tru9I TTAA 3 cut(s) 65, 161, 432
TspGWI ACGGA 1 cut(s) 155
XagI CCTNNNNNAGG 1 cut(s) 205
XceI RCATGY 1 cut(s) 172
Zsp2I ATGCAT 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.