FvH4_2g12280

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016258
Physical Location & Seq
Reverse (-)
10781793 .. 10783232
1440 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g12280.t1

Sequence Viewer

Length: 1440 bp
ATGGAAGATATAGACGAGGAGGGGGATGAGCTTTTGAATCTCAGCCTTGCAATTGTTACAAACTCAGGCGGTGAAAGAATGAAAAAGAGAAAGAGAAGGGATAATACTACTAGCAGCGATTTCAGTTCATCATCATATGAAGGTTGTGAGGGGAAGATATTCAGTCTTCTTCAAATGAGGGAACAAATGTTAAAACTAGAGCACAAGAAGAAACGAGGAGGAGCAGGTAATGATATTGTTGATGAATTAGATGGTAAAGGCCTCCAATTAATCCGCATGTTGCTCATAACAGCCACTGCCGTAAATGAAAACAATGTGAGCTCAGCTTTCGAGAATCTTACAGAGTTGTATAAAAGTGTTTCCTTGTGCGGCGACTCAGTGCAACGAGTTGTGGCTTACTTTGCAGATGGCTTGGCAGCGAGGCTTCTCACCCGAAAATCACCATTCTTCGACATGATTTCCAAGGAGCCAACGCATGAGGAAGAGTTTGTAGCCTTCACTGCTCTCTACCGGGTTTCCCCCTACTACCAGTTTGCTCATTTTACAGCCAACCAGGCCATAATCGAGGCATTTGAGAAAGAAGAAGACAAGAACAACCGTTCCTTGCATGTAATCGATTTCGACGTCTCCTATGGCTTTCAATGGCCTTCATTGATACAATCTCTTTCCGAAAAGGGAAGCAGTGGCAGTCGGATATCCCTTAGGATTACAGGGTTTGGAAGAAGCTTGGACGAACTGCGGGAGACGGAGAATAGATTGCTCAGCTTTTCCAAGGGATGTCGCAACCTCGTCTTTGAATTCCAAGGATTATTGCATGGCTCAAATCTCATAAACTTGAGGAAGAAGAGAAATGAAACAATTGCGGTGAATTTGGTTTTTCATTTGAACAGCTTGAACAATGATATCAAGATATCCGACTCGTTAAAATCTGTACATTTACTTAACCCTTCGATTGTAATGTTGGTTGAACAAGAAGGAAGCAGAAGTCCTCGAAGTTTCTTGTCGAGATTCATGGAGTCTTTGCATTATTTTGCAGCCATGTTTGATTCCTTAGATGATTGCTTACCGCTAGAGAGCACGGAGAGGCTGAGAATCGAGAAGAACCATCTGGGGAAGGAGATCAAAAGCATGCTCAACTACGACAATATTATGGAGGATCGTAAAACAGAAAAGTCGACGGAGACATGGAAAGCGAGGATGGAGAGTCATGGTTTTGAAGGAGTCAATCTAAGCTCCAAGTCCAAGATCCAAGCTAGACTTCTCTTGAAAATTAGGACCCATTATTGTCCCCTTCAATTTGAAGGAGACGGTAGTAATGGTAGCAGTACTGCAGCTGGCTTCAGGGTTTTTGAAAGAGATGATGGAAGGACACTTTCTCTAGGATGGCAAGATAGGTATCTTCTTTCAGTTTCTGCATGGCACTGTCACCGTGTATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

480

Amino Acids

54.47

Weight (kDa)

6.39

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 91 - 473 3.3e-88 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016258)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12280
malus_domestica MD05G1040300.v1.1 MD10G1046300.v1.1
prunus_persica Prupe.8G054000_v2.0.a1
pyrus_communis pycom05g03290
rosa_chinensis RchiOBHm_Chr6g0272211
rosa_laevigata RLG00000013690
rosa_multiflora Rmu_sc0005748.1_g000002
rosa_roxburghii Rroxscaffold_7G00196470
rosa_rugosa Rorug06G0067400
rosa_samantha Rh6BG187000 Rh6CG185100 Rh6DG176800
rosa_wichuraiana Rw0G003840 Rw6G015840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 627
Acc36I ACCTGC 1 cut(s) 215
AccI GTMKAC 1 cut(s) 1175
AciI CCGC 6 cut(s) 69, 274, 369, 739, 863, 1067
AclWI GGATC 2 cut(s) 1164, 1240
AcsI RAATTY 2 cut(s) 797, 868
AcuI CTGAAG 1 cut(s) 1324
AcyI GRCGYC 1 cut(s) 624
AfaI GTAC 2 cut(s) 933, 1327
AjnI CCWGG 1 cut(s) 552
AjuI GAANNNNNNNTTGG 2 cut(s) 1242, 1274
AloI GAACNNNNNNTCC 2 cut(s) 584, 616
AluBI AGCT 9 cut(s) 31, 321, 326, 726, 765, 891, 1233, 1253, 1334
AluI AGCT 9 cut(s) 31, 321, 326, 726, 765, 891, 1233, 1253, 1334
Alw21I GWGCWC 3 cut(s) 204, 323, 1079
Alw26I GTCTC 4 cut(s) 631, 737, 1175, 1299
AlwI GGATC 2 cut(s) 1164, 1240
AlwNI CAGNNNCTG 2 cut(s) 296, 1412
AoxI GGCC 3 cut(s) 259, 555, 644
ApeKI GCWGC 4 cut(s) 114, 416, 1034, 1331
ApoI RAATTY 2 cut(s) 797, 868
AseI ATTAAT 1 cut(s) 269
Asp700I GAANNNNTTC 1 cut(s) 158
AspS9I GGNCC 1 cut(s) 1275
AsuC2I CCSGG 1 cut(s) 512
AsuHPI GGTGA 5 cut(s) 83, 421, 432, 877, 1418
AvaII GGWCC 1 cut(s) 1275
AxyI CCTNAGG 1 cut(s) 701
BanII GRGCYC 1 cut(s) 323
BarI GAAGNNNNNNTAC 2 cut(s) 88, 120
BbsI GAAGAC 2 cut(s) 158, 591
Bbv12I GWGCWC 3 cut(s) 204, 323, 1079
BbvI GCAGC 4 cut(s) 126, 428, 1046, 1343
BccI CCATC 6 cut(s) 245, 401, 1113, 1192, 1355, 1377
BceAI ACGGC 1 cut(s) 284
BciT130I CCWGG 1 cut(s) 554
BcnI CCSGG 1 cut(s) 512
BcoDI GTCTC 4 cut(s) 631, 737, 1175, 1299
BfaI CTAG 5 cut(s) 111, 197, 1070, 1254, 1379
BfmI CTRYAG 1 cut(s) 1329
BfuAI ACCTGC 1 cut(s) 215
BglI GCCNNNNNGGC 1 cut(s) 554
BisI GCNGC 5 cut(s) 115, 370, 417, 1035, 1332
BlpI GCTNAGC 1 cut(s) 322
BlsI GCNGC 5 cut(s) 116, 371, 418, 1036, 1333
BmcAI AGTACT 1 cut(s) 1327
Bme1390I CCNGG 2 cut(s) 512, 554
Bme18I GGWCC 1 cut(s) 1275
BmgT120I GGNCC 1 cut(s) 1275
BmiI GGNNCC 2 cut(s) 468, 1277
BmrFI CCNGG 2 cut(s) 512, 554
BpiI GAAGAC 2 cut(s) 158, 591
Bpu1102I GCTNAGC 2 cut(s) 322, 761
BpuEI CTTGAG 1 cut(s) 856
BpuMI CCSGG 1 cut(s) 512
Bsa29I ATCGAT 1 cut(s) 615
BsaBI GATNNNNATC 1 cut(s) 1395
BsaHI GRCGYC 1 cut(s) 624
BsaJI CCNNGG 3 cut(s) 462, 771, 802
Bse1I ACTGG 1 cut(s) 529
Bse21I CCTNAGG 1 cut(s) 701
Bse8I GATNNNNATC 1 cut(s) 1395
BseBI CCWGG 1 cut(s) 554
BseCI ATCGAT 1 cut(s) 615
BseDI CCNNGG 3 cut(s) 462, 771, 802
BseGI GGATG 4 cut(s) 31, 782, 1203, 1388
BseJI GATNNNNATC 1 cut(s) 1395
BseMII CTCAG 6 cut(s) 55, 78, 336, 390, 775, 1079
BseNI ACTGG 1 cut(s) 529
BseRI GAGGAG 3 cut(s) 32, 231, 234
BseXI GCAGC 4 cut(s) 126, 428, 1046, 1343
BshFI GGCC 3 cut(s) 261, 557, 646
BshVI ATCGAT 1 cut(s) 615
BsiHKAI GWGCWC 3 cut(s) 204, 323, 1079
BsiSI CCGG 1 cut(s) 511
BslFI GGGAC 1 cut(s) 1272
BsmAI GTCTC 4 cut(s) 631, 737, 1175, 1299
BsmBI CGTCTC 3 cut(s) 631, 737, 1299
BsmFI GGGAC 1 cut(s) 1272
BsnI GGCC 3 cut(s) 261, 557, 646
Bsp1286I GDGCHC 3 cut(s) 204, 323, 1079
Bsp1407I TGTACA 1 cut(s) 931
Bsp143I GATC 3 cut(s) 1119, 1156, 1245
Bsp1720I GCTNAGC 2 cut(s) 322, 761
BspACI CCGC 6 cut(s) 69, 274, 369, 739, 863, 1067
BspANI GGCC 3 cut(s) 261, 557, 646
BspCNI CTCAG 6 cut(s) 54, 77, 335, 389, 774, 1080
BspDI ATCGAT 1 cut(s) 615
BspLI GGNNCC 2 cut(s) 468, 1277
BspMAI CTGCAG 1 cut(s) 1333
BspMI ACCTGC 1 cut(s) 215
BspPI GGATC 2 cut(s) 1164, 1240
BsrGI TGTACA 1 cut(s) 931
BsrI ACTGG 1 cut(s) 529
BssECI CCNNGG 3 cut(s) 462, 771, 802
BssMI GATC 3 cut(s) 1119, 1156, 1245
BssNI GRCGYC 1 cut(s) 624
BssT1I CCWWGG 3 cut(s) 462, 771, 802
Bst2UI CCWGG 1 cut(s) 554
Bst4CI ACNGT 4 cut(s) 599, 1310, 1424, 1430
Bst6I CTCTTC 2 cut(s) 477, 839
BstACI GRCGYC 1 cut(s) 624
BstAUI TGTACA 1 cut(s) 931
BstC8I GCNNGC 2 cut(s) 1130, 1336
BstDEI CTNAG 9 cut(s) 41, 64, 322, 376, 701, 761, 1051, 1088, 1229
BstF5I GGATG 4 cut(s) 31, 782, 1203, 1388
BstKTI GATC 3 cut(s) 1122, 1159, 1248
BstMAI GTCTC 4 cut(s) 631, 737, 1175, 1299
BstMBI GATC 3 cut(s) 1119, 1156, 1245
BstMWI GCNNNNNNNGC 3 cut(s) 401, 500, 554
BstNI CCWGG 1 cut(s) 554
BstNSI RCATGY 3 cut(s) 280, 611, 1132
BstSCI CCNGG 2 cut(s) 510, 552
BstSFI CTRYAG 1 cut(s) 1329
BstV1I GCAGC 4 cut(s) 126, 428, 1046, 1343
BstV2I GAAGAC 2 cut(s) 158, 591
BstX2I RGATCY 1 cut(s) 1245
BstYI RGATCY 1 cut(s) 1245
Bsu15I ATCGAT 1 cut(s) 615
Bsu36I CCTNAGG 1 cut(s) 701
BsuRI GGCC 3 cut(s) 261, 557, 646
BsuTUI ATCGAT 1 cut(s) 615
BtsCI GGATG 4 cut(s) 31, 782, 1203, 1388
BtsI GCAGTG 3 cut(s) 294, 498, 688
BtsIMutI CAGTG 5 cut(s) 294, 384, 498, 688, 1420
BveI ACCTGC 1 cut(s) 215
Cac8I GCNNGC 2 cut(s) 1130, 1336
CaiI CAGNNNCTG 2 cut(s) 296, 1412
Cfr13I GGNCC 1 cut(s) 1275
ClaI ATCGAT 1 cut(s) 615
Csp6I GTAC 2 cut(s) 932, 1326
CviQI GTAC 2 cut(s) 932, 1326
DdeI CTNAG 9 cut(s) 41, 64, 322, 376, 701, 761, 1051, 1088, 1229
DpnI GATC 3 cut(s) 1121, 1158, 1247
DpnII GATC 3 cut(s) 1119, 1156, 1245
Eam1104I CTCTTC 2 cut(s) 477, 839
EarI CTCTTC 2 cut(s) 477, 839
Ecl136II GAGCTC 1 cut(s) 321
Eco130I CCWWGG 3 cut(s) 462, 771, 802
Eco147I AGGCCT 1 cut(s) 261
Eco24I GRGCYC 1 cut(s) 323
Eco32I GATATC 3 cut(s) 696, 904, 912
Eco47I GGWCC 1 cut(s) 1275
Eco53kI GAGCTC 1 cut(s) 321
Eco57I CTGAAG 1 cut(s) 1324
Eco81I CCTNAGG 1 cut(s) 701
EcoICRI GAGCTC 1 cut(s) 321
EcoO109I RGGNCCY 1 cut(s) 1275
EcoRI GAATTC 1 cut(s) 797
EcoRII CCWGG 1 cut(s) 552
EcoRV GATATC 3 cut(s) 696, 904, 912
EcoT14I CCWWGG 3 cut(s) 462, 771, 802
EcoT38I GRGCYC 1 cut(s) 323
ErhI CCWWGG 3 cut(s) 462, 771, 802
Esp3I CGTCTC 3 cut(s) 631, 737, 1299
FalI AAGNNNNNCTT 2 cut(s) 1242, 1274
FaqI GGGAC 1 cut(s) 1272
FauI CCCGC 1 cut(s) 732
FauNDI CATATG 1 cut(s) 136
FblI GTMKAC 1 cut(s) 1175
Fnu4HI GCNGC 5 cut(s) 115, 370, 417, 1035, 1332
FokI GGATG 4 cut(s) 38, 789, 1210, 1395
FriOI GRGCYC 1 cut(s) 323
Fsp4HI GCNGC 5 cut(s) 115, 370, 417, 1035, 1332
FspBI CTAG 5 cut(s) 111, 197, 1070, 1254, 1379
GluI GCNGC 5 cut(s) 115, 370, 417, 1035, 1332
HaeIII GGCC 3 cut(s) 261, 557, 646
HapII CCGG 1 cut(s) 511
Hin1I GRCGYC 1 cut(s) 624
HincII GTYRAC 1 cut(s) 1176
HindII GTYRAC 1 cut(s) 1176
HindIII AAGCTT 1 cut(s) 724
HpaII CCGG 1 cut(s) 511
HphI GGTGA 5 cut(s) 83, 421, 432, 877, 1418
Hpy166II GTNNAC 1 cut(s) 1176
Hpy188I TCNGA 3 cut(s) 670, 693, 916
Hpy188III TCNNGA 5 cut(s) 331, 907, 1005, 1096, 1264
Hpy8I GTNNAC 1 cut(s) 1176
Hpy99I CGWCG 2 cut(s) 626, 1180
HpyCH4III ACNGT 4 cut(s) 599, 1310, 1424, 1430
HpyCH4IV ACGT 1 cut(s) 624
HpyCH4V TGCA 9 cut(s) 50, 382, 404, 607, 814, 1024, 1034, 1331, 1415
HpyF10VI GCNNNNNNNGC 3 cut(s) 401, 500, 554
HpyF3I CTNAG 9 cut(s) 41, 64, 322, 376, 701, 761, 1051, 1088, 1229
HpySE526I ACGT 1 cut(s) 624
Hsp92I GRCGYC 1 cut(s) 624
Kzo9I GATC 3 cut(s) 1119, 1156, 1245
LmnI GCTCC 3 cut(s) 221, 466, 1238
Lsp1109I GCAGC 4 cut(s) 126, 428, 1046, 1343
MaeI CTAG 5 cut(s) 111, 197, 1070, 1254, 1379
MaeII ACGT 1 cut(s) 624
MaeIII GTNAC 2 cut(s) 55, 1424
MalI GATC 3 cut(s) 1121, 1158, 1247
MboI GATC 3 cut(s) 1119, 1156, 1245
MfeI CAATTG 2 cut(s) 51, 858
MflI RGATCY 1 cut(s) 1245
MhlI GDGCHC 3 cut(s) 204, 323, 1079
MluCI AATT 8 cut(s) 51, 245, 266, 797, 858, 868, 1269, 1295
MlyI GAGTC 5 cut(s) 368, 911, 1025, 1213, 1230
MmeI TCCRAC 2 cut(s) 671, 939
MroXI GAANNNNTTC 1 cut(s) 158
MseI TTAA 5 cut(s) 191, 269, 923, 942, 1438
MspA1I CMGCKG 1 cut(s) 1334
MspI CCGG 1 cut(s) 511
MspR9I CCNGG 2 cut(s) 512, 554
MunI CAATTG 2 cut(s) 51, 858
MvaI CCWGG 1 cut(s) 554
MwoI GCNNNNNNNGC 3 cut(s) 401, 500, 554
NciI CCSGG 1 cut(s) 512
NdeI CATATG 1 cut(s) 136
NdeII GATC 3 cut(s) 1119, 1156, 1245
NlaIV GGNNCC 2 cut(s) 468, 1277
NmuCI GTSAC 1 cut(s) 1424
NspI RCATGY 3 cut(s) 280, 611, 1132
PaeI GCATGC 1 cut(s) 1132
PceI AGGCCT 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 621
PdmI GAANNNNTTC 1 cut(s) 158
PfeI GAWTC 5 cut(s) 37, 334, 1008, 1046, 1092
PkrI GCNGC 5 cut(s) 116, 371, 418, 1036, 1333
PleI GAGTC 5 cut(s) 368, 911, 1024, 1212, 1229
PpsI GAGTC 5 cut(s) 368, 911, 1024, 1212, 1229
PpuMI RGGWCCY 1 cut(s) 1275
PshBI ATTAAT 1 cut(s) 269
Psp124BI GAGCTC 1 cut(s) 323
Psp5II RGGWCCY 1 cut(s) 1275
Psp6I CCWGG 1 cut(s) 552
PspGI CCWGG 1 cut(s) 552
PspN4I GGNNCC 2 cut(s) 468, 1277
PspPI GGNCC 1 cut(s) 1275
PspPPI RGGWCCY 1 cut(s) 1275
PstI CTGCAG 1 cut(s) 1333
PstNI CAGNNNCTG 2 cut(s) 296, 1412
PsuI RGATCY 1 cut(s) 1245
PvuII CAGCTG 1 cut(s) 1334
RsaI GTAC 2 cut(s) 933, 1327
RsaNI GTAC 2 cut(s) 932, 1326
SacI GAGCTC 1 cut(s) 323
SalI GTCGAC 1 cut(s) 1174
SaqAI TTAA 5 cut(s) 191, 269, 923, 942, 1438
SatI GCNGC 5 cut(s) 115, 370, 417, 1035, 1332
Sau3AI GATC 3 cut(s) 1119, 1156, 1245
Sau96I GGNCC 1 cut(s) 1275
ScaI AGTACT 1 cut(s) 1327
SchI GAGTC 5 cut(s) 368, 911, 1025, 1213, 1230
ScrFI CCNGG 2 cut(s) 512, 554
SduI GDGCHC 3 cut(s) 204, 323, 1079
SfcI CTRYAG 1 cut(s) 1329
SinI GGWCC 1 cut(s) 1275
SmlI CTYRAG 1 cut(s) 835
SmoI CTYRAG 1 cut(s) 835
SphI GCATGC 1 cut(s) 1132
Sse9I AATT 8 cut(s) 51, 245, 266, 797, 858, 868, 1269, 1295
SseBI AGGCCT 1 cut(s) 261
SsiI CCGC 6 cut(s) 69, 274, 369, 739, 863, 1067
SspI AATATT 1 cut(s) 1147
SspMI CTAG 5 cut(s) 111, 197, 1070, 1254, 1379
SstI GAGCTC 1 cut(s) 323
StuI AGGCCT 1 cut(s) 261
StyD4I CCNGG 2 cut(s) 510, 552
StyI CCWWGG 3 cut(s) 462, 771, 802
TaaI ACNGT 4 cut(s) 599, 1310, 1424, 1430
TaiI ACGT 1 cut(s) 627
TasI AATT 8 cut(s) 51, 245, 266, 797, 858, 868, 1269, 1295
TatI WGTACW 2 cut(s) 931, 1325
TauI GCSGC 1 cut(s) 372
TfiI GAWTC 5 cut(s) 37, 334, 1008, 1046, 1092
Tru1I TTAA 5 cut(s) 191, 269, 923, 942, 1438
Tru9I TTAA 5 cut(s) 191, 269, 923, 942, 1438
TscAI CASTG 5 cut(s) 301, 384, 505, 688, 1427
TseFI GTSAC 1 cut(s) 1424
TseI GCWGC 4 cut(s) 114, 416, 1034, 1331
Tsp45I GTSAC 1 cut(s) 1424
TspDTI ATGAA 9 cut(s) 95, 117, 153, 258, 321, 639, 867, 869, 1000
TspGWI ACGGA 3 cut(s) 761, 1094, 1193
TspRI CASTG 5 cut(s) 301, 384, 505, 688, 1427
VpaK11BI GGWCC 1 cut(s) 1275
VspI ATTAAT 1 cut(s) 269
XapI RAATTY 2 cut(s) 797, 868
XceI RCATGY 3 cut(s) 280, 611, 1132
XmiI GTMKAC 1 cut(s) 1175
XmnI GAANNNNTTC 1 cut(s) 158
XspI CTAG 5 cut(s) 111, 197, 1070, 1254, 1379
ZraI GACGTC 1 cut(s) 625
ZrmI AGTACT 1 cut(s) 1327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.