MD05G1040300.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016258
Physical Location & Seq
Forward (+)
6871948 .. 6873466
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1040300.v1.1.491

Sequence Viewer

Length: 1443 bp
ATGCTCATTCATGATCTCTTTCATCAATCAGAGGAGGAGTTTCTGAATCTTAGCCTTGCAATTGTTACAAGTTCAGGGAAGGAGAGATTTAAGAAGAGAAAGAGATCAAGAGACGATGGTATTGCTAATAGTATTTTGGTGAGCCCTCTAAACTCGTATGAAGGTTGTGAAGGGAAGATATTTAGGCTTCTTCAAATGAGGGAACAAATGCTAAAAAAACTAGACCTTCACAAGAAGAAAGGTGTTGCTCTTGATGGTGAAGATGGAAGGGGCCTCCACCTGATCCACATGTTGCTCATAACAGCCACCGCAGTTAACGAAAACAATGTCGGCTCAGCCTTGGAGCATCTCACTGAGTTATACAAGATTGTTTCCTTATGCGGTGACTCTGTCCAACGAGTAGTGGCTTATTTTGCCGACGGCTTAGCTGCAAGGCTTCTCACTCGAAAATCGCCCTTTTATGACATGATCATGAAGGAACCGACGAGTGAAGAAGAGTTTGTAGCGTTCACCGCTCTCTACCGGGTCTCTCCGTACTACCAGTTTGCTCATTTCACGGCAAACCAAGCAATCATTGAGGCATTTGAGAAGGAACAAGAGAAGAACAATCGTGCATTACATGTTATTGATTTTGATGTCTCCTATGGATTTCAGTGGCCTTCTCTTATACAGTCTCTGTCCGAGAACGCAAGCAGTAGCAATCGTATATCGCTCAGAATAACGGGGTTCGGAAGAAGCGTGGATGAGCTACGTGAAACGGAGAACAGACTGACAAGCTTCTCAAAGGGGTTTCGGAATCTTGTTTTTGAATTTCAAGGGTTGTTGAGAGGCTCAAAGCTCATAAACTTGAGAAAAAAGAGAAACGAAACAATTGCTGTGAATTTGGTTTTTCAGTTGAATACATTGAGCAATAATTTGAAGATATCTGACTTGTTAAAATCTGTACATTTACTCAACCCTTCCATTGTAATCTTGGTTGAACAAGAAGGGAGCAAAAGTCCTCGAAGTTTCTTATCGAGATTCATGGAGTCTTTGCATTATTTTGCAGCCATGTTTGATTCTTTAGATGATTGTCTGCCATTAGAGAGTGCTGAAAGGTTGAGCATTGAGAAGAACCACCTTGGAAAAGAGATCAAAAGCATGCTCAATTACGACAATAACAACGATGAGAATTGTCCGAAAACTGAAAAGATGGAAACATGGAAGACGATGATGGAGAGTCAGGGTTTTGAAGGAATAAATCTAAGCTCTAAGTCCAAGATACAAGCAAGACTTCTTTTGAAAATTAGGACTCACTATTCTCCACTTGAGTTTGATGGAGAGAGTAGTGGAGCTGCAGCTGGGTTCAAGGTTTTCGAAAGAGACGATGGAAGGACAATATCTCTTGGGTGGCAAGACAGGTATCTGCTTACAGTTTCTGCATGGCACTGCACTGTATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

481

Amino Acids

54.68

Weight (kDa)

6.91

Isoelectric Point (pI)

41.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 95 - 475 2.8e-90 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016258)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12280
malus_domestica MD05G1040300.v1.1 MD10G1046300.v1.1
prunus_persica Prupe.8G054000_v2.0.a1
pyrus_communis pycom05g03290
rosa_chinensis RchiOBHm_Chr6g0272211
rosa_laevigata RLG00000013690
rosa_multiflora Rmu_sc0005748.1_g000002
rosa_roxburghii Rroxscaffold_7G00196470
rosa_rugosa Rorug06G0067400
rosa_samantha Rh6BG187000 Rh6CG185100 Rh6DG176800
rosa_wichuraiana Rw0G003840 Rw6G015840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 515
AciI CCGC 3 cut(s) 309, 381, 513
AclWI GGATC 1 cut(s) 277
AcsI RAATTY 2 cut(s) 809, 880
AfaI GTAC 2 cut(s) 536, 945
AflIII ACRYGT 2 cut(s) 288, 619
AgsI TTSAA 9 cut(s) 194, 809, 815, 898, 919, 980, 1232, 1282, 1348
AluBI AGCT 7 cut(s) 428, 748, 777, 838, 1248, 1334, 1340
AluI AGCT 7 cut(s) 428, 748, 777, 838, 1248, 1334, 1340
Alw26I GTCTC 5 cut(s) 105, 532, 643, 678, 1356
AlwI GGATC 1 cut(s) 277
AlwNI CAGNNNCTG 2 cut(s) 676, 1418
AoxI GGCC 2 cut(s) 271, 656
ApeKI GCWGC 4 cut(s) 428, 1046, 1334, 1337
ApoI RAATTY 2 cut(s) 809, 880
ArsI GACNNNNNNTTYG 2 cut(s) 312, 344
AspS9I GGNCC 1 cut(s) 271
AsuC2I CCSGG 1 cut(s) 524
AsuHPI GGTGA 4 cut(s) 151, 269, 395, 502
AsuII TTCGAA 1 cut(s) 1356
BanII GRGCYC 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 1211
BbvI GCAGC 4 cut(s) 415, 1058, 1321, 1349
BccI CCATC 7 cut(s) 110, 248, 257, 1186, 1207, 1310, 1361
BceAI ACGGC 2 cut(s) 436, 573
BcgI CGANNNNNNTGC 4 cut(s) 104, 138, 854, 888
BclI TGATCA 1 cut(s) 468
BcnI CCSGG 1 cut(s) 524
BcoDI GTCTC 5 cut(s) 105, 532, 643, 678, 1356
BfaI CTAG 1 cut(s) 221
BfmI CTRYAG 1 cut(s) 1335
BisI GCNGC 4 cut(s) 429, 1047, 1335, 1338
BlpI GCTNAGC 2 cut(s) 334, 424
BlsI GCNGC 4 cut(s) 430, 1048, 1336, 1339
Bme1390I CCNGG 1 cut(s) 524
BmgT120I GGNCC 1 cut(s) 271
BmiI GGNNCC 2 cut(s) 272, 480
BmrFI CCNGG 1 cut(s) 524
BmsI GCATC 1 cut(s) 355
BpiI GAAGAC 1 cut(s) 1211
Bpu1102I GCTNAGC 2 cut(s) 334, 424
Bpu14I TTCGAA 1 cut(s) 1356
BpuEI CTTGAG 2 cut(s) 868, 1328
BpuMI CCSGG 1 cut(s) 524
BsaAI YACGTR 1 cut(s) 752
BsaI GGTCTC 1 cut(s) 532
BsaJI CCNNGG 2 cut(s) 339, 1120
BsaXI ACNNNNNCTCC 2 cut(s) 982, 1012
Bse1I ACTGG 1 cut(s) 541
BseDI CCNNGG 2 cut(s) 339, 1120
BseGI GGATG 1 cut(s) 748
BseMII CTCAG 3 cut(s) 345, 348, 727
BseNI ACTGG 1 cut(s) 541
BseRI GAGGAG 2 cut(s) 47, 50
BseXI GCAGC 4 cut(s) 415, 1058, 1321, 1349
BseYI CCCAGC 1 cut(s) 1340
BsgI GTGCAG 1 cut(s) 1414
BshFI GGCC 2 cut(s) 273, 658
BsiSI CCGG 1 cut(s) 523
BsmAI GTCTC 5 cut(s) 105, 532, 643, 678, 1356
BsmBI CGTCTC 2 cut(s) 105, 1356
BsnI GGCC 2 cut(s) 273, 658
Bso31I GGTCTC 1 cut(s) 532
Bsp119I TTCGAA 1 cut(s) 1356
Bsp1286I GDGCHC 1 cut(s) 146
Bsp1407I TGTACA 1 cut(s) 943
Bsp143I GATC 5 cut(s) 13, 104, 282, 468, 1131
Bsp1720I GCTNAGC 2 cut(s) 334, 424
BspACI CCGC 3 cut(s) 309, 381, 513
BspANI GGCC 2 cut(s) 273, 658
BspCNI CTCAG 3 cut(s) 346, 347, 726
BspHI TCATGA 2 cut(s) 10, 471
BspLI GGNNCC 2 cut(s) 272, 480
BspMAI CTGCAG 1 cut(s) 1339
BspPI GGATC 1 cut(s) 277
BspT104I TTCGAA 1 cut(s) 1356
BspTNI GGTCTC 1 cut(s) 532
BsrBI CCGCTC 1 cut(s) 515
BsrGI TGTACA 1 cut(s) 943
BsrI ACTGG 1 cut(s) 541
BssECI CCNNGG 2 cut(s) 339, 1120
BssMI GATC 5 cut(s) 13, 104, 282, 468, 1131
BssT1I CCWWGG 2 cut(s) 339, 1120
Bst4CI ACNGT 3 cut(s) 672, 1414, 1435
Bst6I CTCTTC 2 cut(s) 89, 489
BstAUI TGTACA 1 cut(s) 943
BstBAI YACGTR 1 cut(s) 752
BstBI TTCGAA 1 cut(s) 1356
BstC8I GCNNGC 2 cut(s) 691, 1142
BstDEI CTNAG 7 cut(s) 50, 334, 354, 424, 713, 1244, 1251
BstF5I GGATG 1 cut(s) 748
BstKTI GATC 5 cut(s) 16, 107, 285, 471, 1134
BstMAI GTCTC 5 cut(s) 105, 532, 643, 678, 1356
BstMBI GATC 5 cut(s) 13, 104, 282, 468, 1131
BstMWI GCNNNNNNNGC 3 cut(s) 413, 512, 566
BstNSI RCATGY 3 cut(s) 292, 623, 1144
BstSCI CCNGG 1 cut(s) 522
BstSFI CTRYAG 1 cut(s) 1335
BstV1I GCAGC 4 cut(s) 415, 1058, 1321, 1349
BstV2I GAAGAC 1 cut(s) 1211
BsuRI GGCC 2 cut(s) 273, 658
BtsCI GGATG 1 cut(s) 748
BtsI GCAGTG 1 cut(s) 1426
BtsIMutI CAGTG 4 cut(s) 351, 659, 1426, 1431
Cac8I GCNNGC 2 cut(s) 691, 1142
CaiI CAGNNNCTG 2 cut(s) 676, 1418
CciI TCATGA 2 cut(s) 10, 471
Cfr13I GGNCC 1 cut(s) 271
Csp6I GTAC 2 cut(s) 535, 944
CviQI GTAC 2 cut(s) 535, 944
DdeI CTNAG 7 cut(s) 50, 334, 354, 424, 713, 1244, 1251
DpnI GATC 5 cut(s) 15, 106, 284, 470, 1133
DpnII GATC 5 cut(s) 13, 104, 282, 468, 1131
Eam1104I CTCTTC 2 cut(s) 89, 489
EarI CTCTTC 2 cut(s) 89, 489
Eco130I CCWWGG 2 cut(s) 339, 1120
Eco24I GRGCYC 1 cut(s) 146
Eco31I GGTCTC 1 cut(s) 532
Eco32I GATATC 1 cut(s) 924
EcoO109I RGGNCCY 1 cut(s) 271
EcoRV GATATC 1 cut(s) 924
EcoT14I CCWWGG 2 cut(s) 339, 1120
EcoT38I GRGCYC 1 cut(s) 146
ErhI CCWWGG 2 cut(s) 339, 1120
Esp3I CGTCTC 2 cut(s) 105, 1356
FalI AAGNNNNNCTT 2 cut(s) 1257, 1289
FbaI TGATCA 1 cut(s) 468
Fnu4HI GCNGC 4 cut(s) 429, 1047, 1335, 1338
FokI GGATG 1 cut(s) 755
FriOI GRGCYC 1 cut(s) 146
Fsp4HI GCNGC 4 cut(s) 429, 1047, 1335, 1338
FspBI CTAG 1 cut(s) 221
GluI GCNGC 4 cut(s) 429, 1047, 1335, 1338
GsaI CCCAGC 1 cut(s) 1344
HaeIII GGCC 2 cut(s) 273, 658
HapII CCGG 1 cut(s) 523
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HindIII AAGCTT 1 cut(s) 775
HinfI GANTC 8 cut(s) 46, 386, 796, 1020, 1028, 1058, 1219, 1291
HpaI GTTAAC 1 cut(s) 316
HpaII CCGG 1 cut(s) 523
HphI GGTGA 4 cut(s) 151, 269, 395, 502
Hpy166II GTNNAC 2 cut(s) 316, 510
Hpy188I TCNGA 8 cut(s) 31, 45, 682, 716, 731, 795, 928, 1179
Hpy188III TCNNGA 5 cut(s) 11, 108, 251, 472, 1017
Hpy8I GTNNAC 2 cut(s) 316, 510
Hpy99I CGWCG 2 cut(s) 422, 487
HpyCH4III ACNGT 3 cut(s) 672, 1414, 1435
HpyCH4IV ACGT 1 cut(s) 751
HpyCH4V TGCA 8 cut(s) 59, 431, 614, 1036, 1046, 1337, 1421, 1431
HpyF10VI GCNNNNNNNGC 3 cut(s) 413, 512, 566
HpyF3I CTNAG 7 cut(s) 50, 334, 354, 424, 713, 1244, 1251
HpySE526I ACGT 1 cut(s) 751
Ksp22I TGATCA 1 cut(s) 468
KspAI GTTAAC 1 cut(s) 316
Kzo9I GATC 5 cut(s) 13, 104, 282, 468, 1131
LmnI GCTCC 3 cut(s) 343, 990, 1331
LpnPI CCDG 7 cut(s) 60, 293, 536, 554, 1208, 1326, 1384
Lsp1109I GCAGC 4 cut(s) 415, 1058, 1321, 1349
LweI GCATC 1 cut(s) 355
MaeI CTAG 1 cut(s) 221
MaeII ACGT 1 cut(s) 751
MaeIII GTNAC 2 cut(s) 64, 383
MalI GATC 5 cut(s) 15, 106, 284, 470, 1133
MbiI CCGCTC 1 cut(s) 515
MboI GATC 5 cut(s) 13, 104, 282, 468, 1131
MfeI CAATTG 2 cut(s) 60, 870
MhlI GDGCHC 1 cut(s) 146
MluCI AATT 8 cut(s) 60, 809, 870, 880, 913, 1147, 1171, 1284
MlyI GAGTC 4 cut(s) 380, 1037, 1228, 1285
MmeI TCCRAC 1 cut(s) 418
MnlI CCTC 8 cut(s) 25, 28, 156, 192, 284, 571, 821, 1011
MseI TTAA 3 cut(s) 90, 315, 935
MslI CAYNNNNRTG 1 cut(s) 470
MspA1I CMGCKG 1 cut(s) 1340
MspI CCGG 1 cut(s) 523
MspR9I CCNGG 1 cut(s) 524
MunI CAATTG 2 cut(s) 60, 870
MwoI GCNNNNNNNGC 3 cut(s) 413, 512, 566
NciI CCSGG 1 cut(s) 524
NdeII GATC 5 cut(s) 13, 104, 282, 468, 1131
NlaIV GGNNCC 2 cut(s) 272, 480
NmuCI GTSAC 1 cut(s) 383
NspI RCATGY 3 cut(s) 292, 623, 1144
NspV TTCGAA 1 cut(s) 1356
PaeI GCATGC 1 cut(s) 1144
PagI TCATGA 2 cut(s) 10, 471
PciI ACATGT 2 cut(s) 288, 619
PcsI WCGNNNNNNNCGW 2 cut(s) 735, 1362
PfeI GAWTC 4 cut(s) 46, 796, 1020, 1058
PflFI GACNNNGTC 1 cut(s) 389
PkrI GCNGC 4 cut(s) 430, 1048, 1336, 1339
PleI GAGTC 4 cut(s) 380, 1036, 1227, 1285
PpsI GAGTC 4 cut(s) 380, 1036, 1227, 1285
Ppu21I YACGTR 1 cut(s) 752
PscI ACATGT 2 cut(s) 288, 619
PspFI CCCAGC 1 cut(s) 1340
PspN4I GGNNCC 2 cut(s) 272, 480
PspPI GGNCC 1 cut(s) 271
PstI CTGCAG 1 cut(s) 1339
PstNI CAGNNNCTG 2 cut(s) 676, 1418
PsyI GACNNNGTC 1 cut(s) 389
PvuII CAGCTG 1 cut(s) 1340
RsaI GTAC 2 cut(s) 536, 945
RsaNI GTAC 2 cut(s) 535, 944
RseI CAYNNNNRTG 1 cut(s) 470
SaqAI TTAA 3 cut(s) 90, 315, 935
SatI GCNGC 4 cut(s) 429, 1047, 1335, 1338
Sau3AI GATC 5 cut(s) 13, 104, 282, 468, 1131
Sau96I GGNCC 1 cut(s) 271
SchI GAGTC 4 cut(s) 380, 1037, 1228, 1285
ScrFI CCNGG 1 cut(s) 524
SduI GDGCHC 1 cut(s) 146
SfaNI GCATC 1 cut(s) 355
SfcI CTRYAG 1 cut(s) 1335
SfuI TTCGAA 1 cut(s) 1356
SmiMI CAYNNNNRTG 1 cut(s) 470
SmlI CTYRAG 2 cut(s) 847, 1307
SmoI CTYRAG 2 cut(s) 847, 1307
SphI GCATGC 1 cut(s) 1144
Sse9I AATT 8 cut(s) 60, 809, 870, 880, 913, 1147, 1171, 1284
SsiI CCGC 3 cut(s) 309, 381, 513
SspMI CTAG 1 cut(s) 221
StyD4I CCNGG 1 cut(s) 522
StyI CCWWGG 2 cut(s) 339, 1120
TaaI ACNGT 3 cut(s) 672, 1414, 1435
TaiI ACGT 1 cut(s) 754
TaqI TCGA 4 cut(s) 445, 1003, 1016, 1356
TasI AATT 8 cut(s) 60, 809, 870, 880, 913, 1147, 1171, 1284
TatI WGTACW 1 cut(s) 943
TfiI GAWTC 4 cut(s) 46, 796, 1020, 1058
Tru1I TTAA 3 cut(s) 90, 315, 935
Tru9I TTAA 3 cut(s) 90, 315, 935
TscAI CASTG 4 cut(s) 358, 659, 1433, 1438
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 4 cut(s) 428, 1046, 1334, 1337
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 4 cut(s) 11, 174, 488, 1012
TspGWI ACGGA 2 cut(s) 522, 773
TspRI CASTG 4 cut(s) 358, 659, 1433, 1438
Tth111I GACNNNGTC 1 cut(s) 389
XapI RAATTY 2 cut(s) 809, 880
XceI RCATGY 3 cut(s) 292, 623, 1144
XcmI CCANNNNNNNNNTGG 1 cut(s) 970
XspI CTAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.