Rroxscaffold_7G00196470

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0016258
Physical Location & Seq
Forward (+)
39473339 .. 39474804
1466 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00196470.1

Sequence Viewer

Length: 1389 bp
ATGGAAGATATAGATGAGGAAGGGGATGAGCTTTTGAATCTCAGCCTAGCAATTGTTACAAACTCAGGCGGTGAAAGAATGAAAAAGAGAAAGAGAAGGGAGAGAAGGGAGAATACTGGCGATCTCAGTTCATCATCATATGAAGGTTGTGAGGGGAAGATATTCAGGCTTCTTCAAATGAGGGAACAAATGCTAAAACTAGACCACAAGAAGAAAGGAGGAGGAGGAGATCACAATTATTGTAATGATAATAATATTTATGGTAGTGTTGATGAAGATGGCAAAGGCCTCCAATTAGTCCACATGTTGCTCATAACAGCCACTGCTGTAAATGAAAACAATGTGAGCTCAGCCTTGGAGAATCTTACAGAGTTGTACAAAAGTGTTTCCTTGTGTGGCGACTCAGTGCAACGAGTTGTGGCTTACTTTGCAGATGGCTTGGCAGCGAGGCTTTTCACCCGGAAATCACCATTCTACGACGTGATTTCCAAGGAGCCAACGCATGAGGAAGAGTTTGTAGCCTTCACTGCTCTTTACCGGGTTTCCCCCTGCTACCAGTTTGCTCATTTTACAGCCAACCAGGCCATAATTGAGGCATTTGAGAAAGAAGAAGACAAGAACAACCGAAGCAGTAGCAGTCGGATATCCCTCAGGATTACGGGGTTTGGAAGACGCTTGGATGAACTGCGGGAGACGGAGAACAGATTGCTCAGTTTTTCAAAGGGATGTCGCAACCTCGTTTTCGAATTCCAAGGATTGCTGCAAGGCTCAAATCTCATAAACTTGAGGAAGAAGAGAAACGAAACGATTGCGGTGAATTTGGTTTTCCATTTGAACAGCTTGAACAATAATATGAAGATATCTGACACATTAAAATCTGTACATTTACTTAACCCTTCGATTGTAATGTTGGTTGAACAAGAAGGAAGCAGAAGCCCTCGAAGTTTCTTGCCGAGATTCATGGAGTCTTTGCATTATTTTGCAGCCATGTTTGATTCTTTAGATGATTGCTTACCACTAGAGAGCGTGGAGAGGCTGAGCATTGAGAAGAACCATCTGGGAAAGGAGATCAAAAGCATGCTCAACTACGACCATATTCAGGAGGATCGAAAAACCGAAAAGTCGACGGAGACATGGAAGGCGAGGATGGAGAGTCATGGTTTTGAAGGAATTAATCTAAGCTCCAAGTCCAAGATCCAAGCTAGACTTCTCTTGAAGATAAGGACCCATTATTGTCCGCTTCAATTCGAAGCAGAGAGTAGTAATGGTAGCAGTACTGCAGCTGGCTTCAGGGTTTTTGAAAGAGATGATGGAAGGACAATTTCTCTAGGATGGCAAGATAGGTTTCTGCTTACAGTTTCTGCATGGCACTGTCACTGTGTGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

462

Amino Acids

52.67

Weight (kDa)

6.56

Isoelectric Point (pI)

47.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 99 - 204 1.4e-22 GRAS domain family
GRAS PF03514 209 - 456 1.2e-45 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016258)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12280
malus_domestica MD05G1040300.v1.1 MD10G1046300.v1.1
prunus_persica Prupe.8G054000_v2.0.a1
pyrus_communis pycom05g03290
rosa_chinensis RchiOBHm_Chr6g0272211
rosa_laevigata RLG00000013690
rosa_multiflora Rmu_sc0005748.1_g000002
rosa_roxburghii Rroxscaffold_7G00196470
rosa_rugosa Rorug06G0067400
rosa_samantha Rh6BG187000 Rh6CG185100 Rh6DG176800
rosa_wichuraiana Rw0G003840 Rw6G015840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1124
AciI CCGC 4 cut(s) 69, 688, 812, 1238
AclWI GGATC 2 cut(s) 1113, 1189
AcsI RAATTY 2 cut(s) 746, 817
AcuI CTGAAG 1 cut(s) 1273
AdeI CACNNNGTG 1 cut(s) 1381
AfaI GTAC 3 cut(s) 377, 882, 1276
AfiI CCNNNNNNNGG 1 cut(s) 1099
AflIII ACRYGT 1 cut(s) 303
AjiI CACGTC 1 cut(s) 481
AjnI CCWGG 1 cut(s) 579
AjuI GAANNNNNNNTTGG 2 cut(s) 1191, 1223
AluBI AGCT 6 cut(s) 31, 348, 840, 1182, 1202, 1283
AluI AGCT 6 cut(s) 31, 348, 840, 1182, 1202, 1283
Alw21I GWGCWC 1 cut(s) 350
Alw26I GTCTC 2 cut(s) 686, 1124
AlwI GGATC 2 cut(s) 1113, 1189
AlwNI CAGNNNCTG 2 cut(s) 323, 1361
AoxI GGCC 2 cut(s) 286, 582
ApeKI GCWGC 4 cut(s) 443, 760, 983, 1280
ApoI RAATTY 2 cut(s) 746, 817
AseI ATTAAT 1 cut(s) 1173
Asp700I GAANNNNTTC 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 1224
AsuC2I CCSGG 2 cut(s) 460, 539
AsuHPI GGTGA 4 cut(s) 83, 448, 459, 826
AsuII TTCGAA 2 cut(s) 744, 1248
AvaII GGWCC 1 cut(s) 1224
AxyI CCTNAGG 1 cut(s) 650
BanII GRGCYC 1 cut(s) 350
BarI GAAGNNNNNNTAC 2 cut(s) 97, 129
BbsI GAAGAC 2 cut(s) 618, 676
Bbv12I GWGCWC 1 cut(s) 350
BbvI GCAGC 4 cut(s) 455, 747, 995, 1292
BccI CCATC 6 cut(s) 272, 428, 1062, 1141, 1304, 1326
BcgI CGANNNNNNTGC 2 cut(s) 791, 825
BciT130I CCWGG 1 cut(s) 581
BcnI CCSGG 2 cut(s) 460, 539
BcoDI GTCTC 2 cut(s) 686, 1124
BfaI CTAG 5 cut(s) 47, 200, 1019, 1203, 1328
BfmI CTRYAG 1 cut(s) 1278
BglI GCCNNNNNGGC 1 cut(s) 581
BisI GCNGC 4 cut(s) 444, 761, 984, 1281
BlpI GCTNAGC 2 cut(s) 349, 1037
BlsI GCNGC 4 cut(s) 445, 762, 985, 1282
BmcAI AGTACT 1 cut(s) 1276
Bme1390I CCNGG 3 cut(s) 460, 539, 581
Bme18I GGWCC 1 cut(s) 1224
BmgBI CACGTC 1 cut(s) 481
BmgT120I GGNCC 1 cut(s) 1224
BmiI GGNNCC 2 cut(s) 495, 1226
BmrFI CCNGG 3 cut(s) 460, 539, 581
BpiI GAAGAC 2 cut(s) 618, 676
Bpu1102I GCTNAGC 2 cut(s) 349, 1037
Bpu14I TTCGAA 2 cut(s) 744, 1248
BpuEI CTTGAG 1 cut(s) 805
BpuMI CCSGG 2 cut(s) 460, 539
BsaJI CCNNGG 3 cut(s) 354, 489, 751
Bsc4I CCNNNNNNNGG 1 cut(s) 1099
Bse1I ACTGG 2 cut(s) 121, 556
Bse21I CCTNAGG 1 cut(s) 650
BseBI CCWGG 1 cut(s) 581
BseDI CCNNGG 3 cut(s) 354, 489, 751
BseGI GGATG 5 cut(s) 31, 685, 731, 1152, 1337
BseLI CCNNNNNNNGG 1 cut(s) 1099
BseMII CTCAG 8 cut(s) 55, 78, 139, 363, 417, 664, 724, 1028
BseNI ACTGG 2 cut(s) 121, 556
BseRI GAGGAG 3 cut(s) 234, 237, 240
BseXI GCAGC 4 cut(s) 455, 747, 995, 1292
BshFI GGCC 2 cut(s) 288, 584
BsiHKAI GWGCWC 1 cut(s) 350
BsiSI CCGG 2 cut(s) 460, 538
BslI CCNNNNNNNGG 1 cut(s) 1099
BsmAI GTCTC 2 cut(s) 686, 1124
BsmBI CGTCTC 1 cut(s) 686
BsnI GGCC 2 cut(s) 288, 584
Bsp119I TTCGAA 2 cut(s) 744, 1248
Bsp1286I GDGCHC 1 cut(s) 350
Bsp1407I TGTACA 2 cut(s) 375, 880
Bsp143I GATC 5 cut(s) 121, 229, 1068, 1105, 1194
Bsp1720I GCTNAGC 2 cut(s) 349, 1037
BspACI CCGC 4 cut(s) 69, 688, 812, 1238
BspANI GGCC 2 cut(s) 288, 584
BspCNI CTCAG 8 cut(s) 54, 77, 138, 362, 416, 663, 723, 1029
BspLI GGNNCC 2 cut(s) 495, 1226
BspMAI CTGCAG 1 cut(s) 1282
BspPI GGATC 2 cut(s) 1113, 1189
BspT104I TTCGAA 2 cut(s) 744, 1248
BsrGI TGTACA 2 cut(s) 375, 880
BsrI ACTGG 2 cut(s) 121, 556
BssECI CCNNGG 3 cut(s) 354, 489, 751
BssMI GATC 5 cut(s) 121, 229, 1068, 1105, 1194
BssT1I CCWWGG 3 cut(s) 354, 489, 751
Bst2UI CCWGG 1 cut(s) 581
Bst4CI ACNGT 3 cut(s) 1357, 1373, 1379
Bst6I CTCTTC 2 cut(s) 504, 788
BstAUI TGTACA 2 cut(s) 375, 880
BstBI TTCGAA 2 cut(s) 744, 1248
BstC8I GCNNGC 2 cut(s) 1079, 1285
BstDEI CTNAG 9 cut(s) 41, 64, 125, 349, 403, 650, 710, 1037, 1178
BstF5I GGATG 5 cut(s) 31, 685, 731, 1152, 1337
BstKTI GATC 5 cut(s) 124, 232, 1071, 1108, 1197
BstMAI GTCTC 2 cut(s) 686, 1124
BstMBI GATC 5 cut(s) 121, 229, 1068, 1105, 1194
BstMWI GCNNNNNNNGC 3 cut(s) 428, 527, 581
BstNI CCWGG 1 cut(s) 581
BstNSI RCATGY 2 cut(s) 307, 1081
BstSCI CCNGG 3 cut(s) 458, 537, 579
BstSFI CTRYAG 1 cut(s) 1278
BstV1I GCAGC 4 cut(s) 455, 747, 995, 1292
BstV2I GAAGAC 2 cut(s) 618, 676
BstX2I RGATCY 1 cut(s) 1194
BstYI RGATCY 1 cut(s) 1194
Bsu36I CCTNAGG 1 cut(s) 650
BsuRI GGCC 2 cut(s) 288, 584
BtrI CACGTC 1 cut(s) 481
BtsCI GGATG 5 cut(s) 31, 685, 731, 1152, 1337
BtsI GCAGTG 2 cut(s) 321, 525
BtsIMutI CAGTG 5 cut(s) 321, 411, 525, 1369, 1375
Cac8I GCNNGC 2 cut(s) 1079, 1285
CaiI CAGNNNCTG 2 cut(s) 323, 1361
Cfr13I GGNCC 1 cut(s) 1224
CseI GACGC 1 cut(s) 681
Csp6I GTAC 3 cut(s) 376, 881, 1275
CviAII CATG 8 cut(s) 304, 503, 961, 988, 1078, 1134, 1157, 1365
CviQI GTAC 3 cut(s) 376, 881, 1275
DdeI CTNAG 9 cut(s) 41, 64, 125, 349, 403, 650, 710, 1037, 1178
DpnI GATC 5 cut(s) 123, 231, 1070, 1107, 1196
DpnII GATC 5 cut(s) 121, 229, 1068, 1105, 1194
DraIII CACNNNGTG 1 cut(s) 1381
Eam1104I CTCTTC 2 cut(s) 504, 788
EarI CTCTTC 2 cut(s) 504, 788
Ecl136II GAGCTC 1 cut(s) 348
Eco130I CCWWGG 3 cut(s) 354, 489, 751
Eco147I AGGCCT 1 cut(s) 288
Eco24I GRGCYC 1 cut(s) 350
Eco32I GATATC 2 cut(s) 645, 861
Eco47I GGWCC 1 cut(s) 1224
Eco53kI GAGCTC 1 cut(s) 348
Eco57I CTGAAG 1 cut(s) 1273
Eco81I CCTNAGG 1 cut(s) 650
EcoICRI GAGCTC 1 cut(s) 348
EcoO109I RGGNCCY 1 cut(s) 1224
EcoRI GAATTC 1 cut(s) 746
EcoRII CCWGG 1 cut(s) 579
EcoRV GATATC 2 cut(s) 645, 861
EcoT14I CCWWGG 3 cut(s) 354, 489, 751
EcoT38I GRGCYC 1 cut(s) 350
ErhI CCWWGG 3 cut(s) 354, 489, 751
Esp3I CGTCTC 1 cut(s) 686
FaeI CATG 8 cut(s) 307, 506, 964, 991, 1081, 1137, 1160, 1368
FalI AAGNNNNNCTT 2 cut(s) 1191, 1223
FatI CATG 8 cut(s) 303, 502, 960, 987, 1077, 1133, 1156, 1364
FauI CCCGC 1 cut(s) 681
FauNDI CATATG 1 cut(s) 139
FblI GTMKAC 1 cut(s) 1124
Fnu4HI GCNGC 4 cut(s) 444, 761, 984, 1281
FokI GGATG 5 cut(s) 38, 692, 738, 1159, 1344
FriOI GRGCYC 1 cut(s) 350
Fsp4HI GCNGC 4 cut(s) 444, 761, 984, 1281
FspBI CTAG 5 cut(s) 47, 200, 1019, 1203, 1328
GluI GCNGC 4 cut(s) 444, 761, 984, 1281
HaeIII GGCC 2 cut(s) 288, 584
HapII CCGG 2 cut(s) 460, 538
HgaI GACGC 1 cut(s) 681
Hin1II CATG 8 cut(s) 307, 506, 964, 991, 1081, 1137, 1160, 1368
HincII GTYRAC 1 cut(s) 1125
HindII GTYRAC 1 cut(s) 1125
HinfI GANTC 7 cut(s) 37, 361, 401, 957, 965, 995, 1153
HpaII CCGG 2 cut(s) 460, 538
HphI GGTGA 4 cut(s) 83, 448, 459, 826
Hpy166II GTNNAC 2 cut(s) 301, 1125
Hpy188I TCNGA 2 cut(s) 642, 865
Hpy188III TCNNGA 3 cut(s) 652, 1100, 1213
Hpy8I GTNNAC 2 cut(s) 301, 1125
Hpy99I CGWCG 2 cut(s) 482, 1129
HpyCH4III ACNGT 3 cut(s) 1357, 1373, 1379
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 7 cut(s) 409, 431, 763, 973, 983, 1280, 1364
HpyF10VI GCNNNNNNNGC 3 cut(s) 428, 527, 581
HpyF3I CTNAG 9 cut(s) 41, 64, 125, 349, 403, 650, 710, 1037, 1178
HpySE526I ACGT 1 cut(s) 480
Hsp92II CATG 8 cut(s) 307, 506, 964, 991, 1081, 1137, 1160, 1368
Kzo9I GATC 5 cut(s) 121, 229, 1068, 1105, 1194
LmnI GCTCC 2 cut(s) 493, 1187
Lsp1109I GCAGC 4 cut(s) 455, 747, 995, 1292
MaeI CTAG 5 cut(s) 47, 200, 1019, 1203, 1328
MaeII ACGT 1 cut(s) 480
MaeIII GTNAC 2 cut(s) 55, 1373
MalI GATC 5 cut(s) 123, 231, 1070, 1107, 1196
MboI GATC 5 cut(s) 121, 229, 1068, 1105, 1194
MfeI CAATTG 1 cut(s) 51
MflI RGATCY 1 cut(s) 1194
MhlI GDGCHC 1 cut(s) 350
MluCI AATT 9 cut(s) 51, 235, 293, 588, 746, 817, 1170, 1244, 1320
MlyI GAGTC 3 cut(s) 395, 974, 1162
MmeI TCCRAC 1 cut(s) 620
MroXI GAANNNNTTC 1 cut(s) 161
MseI TTAA 3 cut(s) 872, 891, 1173
MspA1I CMGCKG 1 cut(s) 1283
MspI CCGG 2 cut(s) 460, 538
MspR9I CCNGG 3 cut(s) 460, 539, 581
MunI CAATTG 1 cut(s) 51
MvaI CCWGG 1 cut(s) 581
MwoI GCNNNNNNNGC 3 cut(s) 428, 527, 581
NciI CCSGG 2 cut(s) 460, 539
NdeI CATATG 1 cut(s) 139
NdeII GATC 5 cut(s) 121, 229, 1068, 1105, 1194
NlaIII CATG 8 cut(s) 307, 506, 964, 991, 1081, 1137, 1160, 1368
NlaIV GGNNCC 2 cut(s) 495, 1226
NmeAIII GCCGAG 1 cut(s) 978
NmuCI GTSAC 1 cut(s) 1373
NspI RCATGY 2 cut(s) 307, 1081
NspV TTCGAA 2 cut(s) 744, 1248
PaeI GCATGC 1 cut(s) 1081
PceI AGGCCT 1 cut(s) 288
PciI ACATGT 1 cut(s) 303
PdmI GAANNNNTTC 1 cut(s) 161
PfeI GAWTC 4 cut(s) 37, 361, 957, 995
PkrI GCNGC 4 cut(s) 445, 762, 985, 1282
PleI GAGTC 3 cut(s) 395, 973, 1161
PpsI GAGTC 3 cut(s) 395, 973, 1161
PpuMI RGGWCCY 1 cut(s) 1224
PscI ACATGT 1 cut(s) 303
PshBI ATTAAT 1 cut(s) 1173
Psp124BI GAGCTC 1 cut(s) 350
Psp5II RGGWCCY 1 cut(s) 1224
Psp6I CCWGG 1 cut(s) 579
PspGI CCWGG 1 cut(s) 579
PspN4I GGNNCC 2 cut(s) 495, 1226
PspPI GGNCC 1 cut(s) 1224
PspPPI RGGWCCY 1 cut(s) 1224
PstI CTGCAG 1 cut(s) 1282
PstNI CAGNNNCTG 2 cut(s) 323, 1361
PsuI RGATCY 1 cut(s) 1194
PvuII CAGCTG 1 cut(s) 1283
RsaI GTAC 3 cut(s) 377, 882, 1276
RsaNI GTAC 3 cut(s) 376, 881, 1275
SacI GAGCTC 1 cut(s) 350
SalI GTCGAC 1 cut(s) 1123
SaqAI TTAA 3 cut(s) 872, 891, 1173
SatI GCNGC 4 cut(s) 444, 761, 984, 1281
Sau3AI GATC 5 cut(s) 121, 229, 1068, 1105, 1194
Sau96I GGNCC 1 cut(s) 1224
ScaI AGTACT 1 cut(s) 1276
SchI GAGTC 3 cut(s) 395, 974, 1162
ScrFI CCNGG 3 cut(s) 460, 539, 581
SduI GDGCHC 1 cut(s) 350
SfcI CTRYAG 1 cut(s) 1278
SfuI TTCGAA 2 cut(s) 744, 1248
SinI GGWCC 1 cut(s) 1224
SmlI CTYRAG 1 cut(s) 784
SmoI CTYRAG 1 cut(s) 784
SphI GCATGC 1 cut(s) 1081
Sse9I AATT 9 cut(s) 51, 235, 293, 588, 746, 817, 1170, 1244, 1320
SseBI AGGCCT 1 cut(s) 288
SsiI CCGC 4 cut(s) 69, 688, 812, 1238
SspI AATATT 1 cut(s) 256
SspMI CTAG 5 cut(s) 47, 200, 1019, 1203, 1328
SstI GAGCTC 1 cut(s) 350
StuI AGGCCT 1 cut(s) 288
StyD4I CCNGG 3 cut(s) 458, 537, 579
StyI CCWWGG 3 cut(s) 354, 489, 751
TaaI ACNGT 3 cut(s) 1357, 1373, 1379
TaiI ACGT 1 cut(s) 483
TaqI TCGA 6 cut(s) 744, 899, 940, 1108, 1124, 1248
TasI AATT 9 cut(s) 51, 235, 293, 588, 746, 817, 1170, 1244, 1320
TatI WGTACW 3 cut(s) 375, 880, 1274
TfiI GAWTC 4 cut(s) 37, 361, 957, 995
Tru1I TTAA 3 cut(s) 872, 891, 1173
Tru9I TTAA 3 cut(s) 872, 891, 1173
TscAI CASTG 5 cut(s) 328, 411, 532, 1376, 1382
TseFI GTSAC 1 cut(s) 1373
TseI GCWGC 4 cut(s) 443, 760, 983, 1280
Tsp45I GTSAC 1 cut(s) 1373
TspDTI ATGAA 8 cut(s) 95, 120, 156, 288, 348, 696, 869, 949
TspGWI ACGGA 2 cut(s) 710, 1142
TspRI CASTG 5 cut(s) 328, 411, 532, 1376, 1382
VpaK11BI GGWCC 1 cut(s) 1224
VspI ATTAAT 1 cut(s) 1173
XapI RAATTY 2 cut(s) 746, 817
XceI RCATGY 2 cut(s) 307, 1081
XmiI GTMKAC 1 cut(s) 1124
XmnI GAANNNNTTC 1 cut(s) 161
XspI CTAG 5 cut(s) 47, 200, 1019, 1203, 1328
ZrmI AGTACT 1 cut(s) 1276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.