Rw0G003840

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00144
OG0016258
Physical Location & Seq
Forward (+)
4948 .. 6354
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G003840.1

Sequence Viewer

Length: 1407 bp
ATGGAAGATATAGATGAGGAAGGGGATGAGCTTTTGAAACTCAGCCTAGCAATTGTTACAAACTCAGGCGGTGAAAGAATGAAAAAGAGAAAGAGAAGGGAGAATACTGGCGATCTCAGTTCATCATCATATGAAGGTTGTGAGGGGAAAATATTCAGGCTTCTTCAAATGAGGGAACAAATGCTAAAACTAGACCACAAGAAGAAAGGAGGAGGAGGAGATCTCAATTATTGTAATGATAATAATATTTATGGTAGTGTTGATGAAGATGGCAAAGGCCTCCAATTAGTCCACATGTTGCTCATAACAGCCACTGCTGTAAATGAAAACAATGTGAGCTCAGCCTTGGAGAATCTTACAGAGTTGTACAAAAGTGTTTCCTTGTGTGGCGACTCAGTGCAACGAGTTGTGGCTTACTTTGCAGATGGCTTGGCGGCGAGGCTTCTCACCCGGAAATCACCATTCTACGACGTGATTTCCAAGGAGCCAACACATGATGAAGAGTTTGTAGCCTTCACTGCTCTTTACCGAGTTTCCCCCTACTACCAGTTTGCTCATTTTACAGCCAACCAGGCCATAATTGAGTCATTTGAGAAAGAAGAAGACAAGAACAACCGTTCCTTGCATATAATTGATTTCGACGTCTCCTATGGCTTTCAATGGCCTTCACTACAATCTCTTTCCGAAAAGGGAAGCAGTAGCAGTCGGATATCCCTCAGGATTACGGGGTTTGGAAGAAGCTTGGATGAACTGCGGGAGACGGAGAACAGATTGCTCAGTTTTTCAAAGGGATGTCGTAACCTCGTTTTCGAATTCCAAGGATTGCTGCAAGGCTCAAATCTCATAAACTTGAGGAAGAAGAGAAACGAAACGATTGCGGTGAATTTGGTTTTCCATTTGAACAGCTTGAACAATAATATGAAGATATCTGACACATTAAAATCTGTACATTTACTTAACCCTTCGATTGTAATGTTGGTTGAACAAGAAGGAAGCAGAAGCCCTCGAAGTTTCTTGCCGAGATTCATGGAGTCTTTGCATTATTTTGCAGCCATGTTTGATTCTTTAGATGATTGCTTACCACTAGAGAGCTTGGAGAGGCTGAGCATTGAGAAGAACCATCTGGGAAAGGAGATCAAAAGCATGCTCAACTACGACCATATTCAGGAGGATCGAAAAACCGAAAAGTCGACGGAGACATGGAAGGCGAGGATGGAGAGTTATGGTTTTGAAGGAATTAATCTAAGCTCCAAGTCCAAGATCCAAGCTAGACTTCTCTTGAAGATAGGACCCATTATTGTCCGCTTCAATTCGAAGGAGAGAGTAGTAATGGTAGCAGTACTGCAGCTGGCTTCAGAGTTTTTTGAAAGAGATGATGGACGGACAATTTCTCTAGGAGGCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

468

Amino Acids

53.16

Weight (kDa)

6.27

Isoelectric Point (pI)

44.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 96 - 428 9.1e-82 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016258)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12280
malus_domestica MD05G1040300.v1.1 MD10G1046300.v1.1
prunus_persica Prupe.8G054000_v2.0.a1
pyrus_communis pycom05g03290
rosa_chinensis RchiOBHm_Chr6g0272211
rosa_laevigata RLG00000013690
rosa_multiflora Rmu_sc0005748.1_g000002
rosa_roxburghii Rroxscaffold_7G00196470
rosa_rugosa Rorug06G0067400
rosa_samantha Rh6BG187000 Rh6CG185100 Rh6DG176800
rosa_wichuraiana Rw0G003840 Rw6G015840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 645
AccI GTMKAC 1 cut(s) 1190
AciI CCGC 5 cut(s) 69, 434, 754, 878, 1303
AclWI GGATC 2 cut(s) 1179, 1255
AcsI RAATTY 2 cut(s) 812, 883
AcuI CTGAAG 1 cut(s) 1338
AcyI GRCGYC 1 cut(s) 642
AfaI GTAC 3 cut(s) 368, 948, 1341
AfiI CCNNNNNNNGG 1 cut(s) 1165
AflIII ACRYGT 1 cut(s) 294
AjiI CACGTC 1 cut(s) 472
AjnI CCWGG 1 cut(s) 570
AjuI GAANNNNNNNTTGG 2 cut(s) 1257, 1289
AloI GAACNNNNNNTCC 2 cut(s) 602, 634
AluBI AGCT 8 cut(s) 31, 339, 741, 906, 1092, 1248, 1268, 1348
AluI AGCT 8 cut(s) 31, 339, 741, 906, 1092, 1248, 1268, 1348
Alw21I GWGCWC 1 cut(s) 341
Alw26I GTCTC 3 cut(s) 649, 752, 1190
AlwI GGATC 2 cut(s) 1179, 1255
AlwNI CAGNNNCTG 1 cut(s) 314
AoxI GGCC 3 cut(s) 277, 573, 662
ApeKI GCWGC 3 cut(s) 826, 1049, 1345
ApoI RAATTY 2 cut(s) 812, 883
AseI ATTAAT 1 cut(s) 1239
Asp700I GAANNNNTTC 1 cut(s) 152
AspS9I GGNCC 1 cut(s) 1289
AsuC2I CCSGG 1 cut(s) 451
AsuHPI GGTGA 4 cut(s) 83, 439, 450, 892
AsuII TTCGAA 2 cut(s) 810, 1313
AvaII GGWCC 1 cut(s) 1289
AxyI CCTNAGG 1 cut(s) 716
BanII GRGCYC 1 cut(s) 341
BarI GAAGNNNNNNTAC 2 cut(s) 88, 120
BbsI GAAGAC 1 cut(s) 609
Bbv12I GWGCWC 1 cut(s) 341
BbvI GCAGC 3 cut(s) 813, 1061, 1357
BccI CCATC 5 cut(s) 263, 419, 1128, 1207, 1370
BcgI CGANNNNNNTGC 2 cut(s) 857, 891
BciT130I CCWGG 1 cut(s) 572
BcnI CCSGG 1 cut(s) 451
BcoDI GTCTC 3 cut(s) 649, 752, 1190
BfaI CTAG 5 cut(s) 47, 191, 1085, 1269, 1394
BfmI CTRYAG 1 cut(s) 1343
BglI GCCNNNNNGGC 1 cut(s) 572
BglII AGATCT 1 cut(s) 220
BisI GCNGC 4 cut(s) 435, 827, 1050, 1346
BlpI GCTNAGC 2 cut(s) 340, 1103
BlsI GCNGC 4 cut(s) 436, 828, 1051, 1347
BmcAI AGTACT 1 cut(s) 1341
Bme1390I CCNGG 2 cut(s) 451, 572
Bme18I GGWCC 1 cut(s) 1289
BmgBI CACGTC 1 cut(s) 472
BmgT120I GGNCC 1 cut(s) 1289
BmiI GGNNCC 2 cut(s) 486, 1291
BmrFI CCNGG 2 cut(s) 451, 572
BpiI GAAGAC 1 cut(s) 609
BplI GAGNNNNNCTC 2 cut(s) 207, 239
Bpu1102I GCTNAGC 2 cut(s) 340, 1103
Bpu14I TTCGAA 2 cut(s) 810, 1313
BpuEI CTTGAG 1 cut(s) 871
BpuMI CCSGG 1 cut(s) 451
BsaHI GRCGYC 1 cut(s) 642
BsaJI CCNNGG 3 cut(s) 345, 480, 817
Bsc4I CCNNNNNNNGG 1 cut(s) 1165
Bse1I ACTGG 2 cut(s) 112, 547
Bse21I CCTNAGG 1 cut(s) 716
BseBI CCWGG 1 cut(s) 572
BseDI CCNNGG 3 cut(s) 345, 480, 817
BseGI GGATG 4 cut(s) 31, 751, 797, 1218
BseLI CCNNNNNNNGG 1 cut(s) 1165
BseMII CTCAG 8 cut(s) 55, 78, 130, 354, 408, 730, 790, 1094
BseNI ACTGG 2 cut(s) 112, 547
BseRI GAGGAG 3 cut(s) 225, 228, 231
BseXI GCAGC 3 cut(s) 813, 1061, 1357
BshFI GGCC 3 cut(s) 279, 575, 664
BsiHKAI GWGCWC 1 cut(s) 341
BsiSI CCGG 1 cut(s) 451
BslI CCNNNNNNNGG 1 cut(s) 1165
BsmAI GTCTC 3 cut(s) 649, 752, 1190
BsmBI CGTCTC 2 cut(s) 649, 752
BsnI GGCC 3 cut(s) 279, 575, 664
Bsp119I TTCGAA 2 cut(s) 810, 1313
Bsp1286I GDGCHC 1 cut(s) 341
Bsp1407I TGTACA 2 cut(s) 366, 946
Bsp143I GATC 5 cut(s) 112, 220, 1134, 1171, 1260
Bsp1720I GCTNAGC 2 cut(s) 340, 1103
BspACI CCGC 5 cut(s) 69, 434, 754, 878, 1303
BspANI GGCC 3 cut(s) 279, 575, 664
BspCNI CTCAG 8 cut(s) 54, 77, 129, 353, 407, 729, 789, 1095
BspLI GGNNCC 2 cut(s) 486, 1291
BspMAI CTGCAG 1 cut(s) 1347
BspPI GGATC 2 cut(s) 1179, 1255
BspT104I TTCGAA 2 cut(s) 810, 1313
BsrGI TGTACA 2 cut(s) 366, 946
BsrI ACTGG 2 cut(s) 112, 547
BssECI CCNNGG 3 cut(s) 345, 480, 817
BssMI GATC 5 cut(s) 112, 220, 1134, 1171, 1260
BssNI GRCGYC 1 cut(s) 642
BssT1I CCWWGG 3 cut(s) 345, 480, 817
Bst2UI CCWGG 1 cut(s) 572
Bst4CI ACNGT 1 cut(s) 617
Bst6I CTCTTC 2 cut(s) 495, 854
BstACI GRCGYC 1 cut(s) 642
BstAUI TGTACA 2 cut(s) 366, 946
BstBI TTCGAA 2 cut(s) 810, 1313
BstC8I GCNNGC 2 cut(s) 1145, 1350
BstDEI CTNAG 9 cut(s) 41, 64, 116, 340, 394, 716, 776, 1103, 1244
BstF5I GGATG 4 cut(s) 31, 751, 797, 1218
BstKTI GATC 5 cut(s) 115, 223, 1137, 1174, 1263
BstMAI GTCTC 3 cut(s) 649, 752, 1190
BstMBI GATC 5 cut(s) 112, 220, 1134, 1171, 1260
BstMWI GCNNNNNNNGC 3 cut(s) 419, 518, 572
BstNI CCWGG 1 cut(s) 572
BstNSI RCATGY 2 cut(s) 298, 1147
BstSCI CCNGG 2 cut(s) 449, 570
BstSFI CTRYAG 1 cut(s) 1343
BstV1I GCAGC 3 cut(s) 813, 1061, 1357
BstV2I GAAGAC 1 cut(s) 609
BstX2I RGATCY 2 cut(s) 220, 1260
BstYI RGATCY 2 cut(s) 220, 1260
Bsu36I CCTNAGG 1 cut(s) 716
BsuRI GGCC 3 cut(s) 279, 575, 664
BtrI CACGTC 1 cut(s) 472
BtsCI GGATG 4 cut(s) 31, 751, 797, 1218
BtsI GCAGTG 2 cut(s) 312, 516
BtsIMutI CAGTG 3 cut(s) 312, 402, 516
Cac8I GCNNGC 2 cut(s) 1145, 1350
CaiI CAGNNNCTG 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 1289
Csp6I GTAC 3 cut(s) 367, 947, 1340
CviAII CATG 6 cut(s) 295, 494, 1027, 1054, 1144, 1200
CviQI GTAC 3 cut(s) 367, 947, 1340
DdeI CTNAG 9 cut(s) 41, 64, 116, 340, 394, 716, 776, 1103, 1244
DpnI GATC 5 cut(s) 114, 222, 1136, 1173, 1262
DpnII GATC 5 cut(s) 112, 220, 1134, 1171, 1260
Eam1104I CTCTTC 2 cut(s) 495, 854
EarI CTCTTC 2 cut(s) 495, 854
Ecl136II GAGCTC 1 cut(s) 339
Eco130I CCWWGG 3 cut(s) 345, 480, 817
Eco147I AGGCCT 1 cut(s) 279
Eco24I GRGCYC 1 cut(s) 341
Eco32I GATATC 2 cut(s) 711, 927
Eco47I GGWCC 1 cut(s) 1289
Eco53kI GAGCTC 1 cut(s) 339
Eco57I CTGAAG 1 cut(s) 1338
Eco81I CCTNAGG 1 cut(s) 716
EcoICRI GAGCTC 1 cut(s) 339
EcoO109I RGGNCCY 1 cut(s) 1289
EcoRI GAATTC 1 cut(s) 812
EcoRII CCWGG 1 cut(s) 570
EcoRV GATATC 2 cut(s) 711, 927
EcoT14I CCWWGG 3 cut(s) 345, 480, 817
EcoT38I GRGCYC 1 cut(s) 341
ErhI CCWWGG 3 cut(s) 345, 480, 817
Esp3I CGTCTC 2 cut(s) 649, 752
FaeI CATG 6 cut(s) 298, 497, 1030, 1057, 1147, 1203
FalI AAGNNNNNCTT 2 cut(s) 1257, 1289
FatI CATG 6 cut(s) 294, 493, 1026, 1053, 1143, 1199
FauI CCCGC 1 cut(s) 747
FauNDI CATATG 1 cut(s) 130
FblI GTMKAC 1 cut(s) 1190
Fnu4HI GCNGC 4 cut(s) 435, 827, 1050, 1346
FokI GGATG 4 cut(s) 38, 758, 804, 1225
FriOI GRGCYC 1 cut(s) 341
Fsp4HI GCNGC 4 cut(s) 435, 827, 1050, 1346
FspBI CTAG 5 cut(s) 47, 191, 1085, 1269, 1394
GluI GCNGC 4 cut(s) 435, 827, 1050, 1346
HaeIII GGCC 3 cut(s) 279, 575, 664
HapII CCGG 1 cut(s) 451
Hin1I GRCGYC 1 cut(s) 642
Hin1II CATG 6 cut(s) 298, 497, 1030, 1057, 1147, 1203
HincII GTYRAC 1 cut(s) 1191
HindII GTYRAC 1 cut(s) 1191
HindIII AAGCTT 1 cut(s) 739
HinfI GANTC 6 cut(s) 352, 392, 584, 1023, 1031, 1061
HpaII CCGG 1 cut(s) 451
HphI GGTGA 4 cut(s) 83, 439, 450, 892
Hpy166II GTNNAC 2 cut(s) 292, 1191
Hpy188I TCNGA 4 cut(s) 685, 708, 931, 1357
Hpy188III TCNNGA 3 cut(s) 718, 1166, 1279
Hpy8I GTNNAC 2 cut(s) 292, 1191
Hpy99I CGWCG 3 cut(s) 473, 644, 1195
HpyCH4III ACNGT 1 cut(s) 617
HpyCH4IV ACGT 2 cut(s) 471, 642
HpyCH4V TGCA 7 cut(s) 400, 422, 625, 829, 1039, 1049, 1345
HpyF10VI GCNNNNNNNGC 3 cut(s) 419, 518, 572
HpyF3I CTNAG 9 cut(s) 41, 64, 116, 340, 394, 716, 776, 1103, 1244
HpySE526I ACGT 2 cut(s) 471, 642
Hsp92I GRCGYC 1 cut(s) 642
Hsp92II CATG 6 cut(s) 298, 497, 1030, 1057, 1147, 1203
Kzo9I GATC 5 cut(s) 112, 220, 1134, 1171, 1260
LmnI GCTCC 2 cut(s) 484, 1253
Lsp1109I GCAGC 3 cut(s) 813, 1061, 1357
MaeI CTAG 5 cut(s) 47, 191, 1085, 1269, 1394
MaeII ACGT 2 cut(s) 471, 642
MaeIII GTNAC 2 cut(s) 55, 797
MalI GATC 5 cut(s) 114, 222, 1136, 1173, 1262
MboI GATC 5 cut(s) 112, 220, 1134, 1171, 1260
MfeI CAATTG 1 cut(s) 51
MflI RGATCY 2 cut(s) 220, 1260
MhlI GDGCHC 1 cut(s) 341
MlyI GAGTC 3 cut(s) 386, 593, 1040
MmeI TCCRAC 1 cut(s) 686
MroXI GAANNNNTTC 1 cut(s) 152
MseI TTAA 3 cut(s) 938, 957, 1239
MspA1I CMGCKG 1 cut(s) 1348
MspI CCGG 1 cut(s) 451
MspR9I CCNGG 2 cut(s) 451, 572
MunI CAATTG 1 cut(s) 51
MvaI CCWGG 1 cut(s) 572
MwoI GCNNNNNNNGC 3 cut(s) 419, 518, 572
NciI CCSGG 1 cut(s) 451
NdeI CATATG 1 cut(s) 130
NdeII GATC 5 cut(s) 112, 220, 1134, 1171, 1260
NlaIII CATG 6 cut(s) 298, 497, 1030, 1057, 1147, 1203
NlaIV GGNNCC 2 cut(s) 486, 1291
NmeAIII GCCGAG 1 cut(s) 1044
NspI RCATGY 2 cut(s) 298, 1147
NspV TTCGAA 2 cut(s) 810, 1313
PaeI GCATGC 1 cut(s) 1147
PceI AGGCCT 1 cut(s) 279
PciI ACATGT 1 cut(s) 294
PdmI GAANNNNTTC 1 cut(s) 152
PfeI GAWTC 3 cut(s) 352, 1023, 1061
PkrI GCNGC 4 cut(s) 436, 828, 1051, 1347
PleI GAGTC 3 cut(s) 386, 592, 1039
PpsI GAGTC 3 cut(s) 386, 592, 1039
PpuMI RGGWCCY 1 cut(s) 1289
PscI ACATGT 1 cut(s) 294
PshBI ATTAAT 1 cut(s) 1239
Psp124BI GAGCTC 1 cut(s) 341
Psp5II RGGWCCY 1 cut(s) 1289
Psp6I CCWGG 1 cut(s) 570
PspGI CCWGG 1 cut(s) 570
PspN4I GGNNCC 2 cut(s) 486, 1291
PspPI GGNCC 1 cut(s) 1289
PspPPI RGGWCCY 1 cut(s) 1289
PstI CTGCAG 1 cut(s) 1347
PstNI CAGNNNCTG 1 cut(s) 314
PsuI RGATCY 2 cut(s) 220, 1260
PvuII CAGCTG 1 cut(s) 1348
RsaI GTAC 3 cut(s) 368, 948, 1341
RsaNI GTAC 3 cut(s) 367, 947, 1340
SacI GAGCTC 1 cut(s) 341
SalI GTCGAC 1 cut(s) 1189
SaqAI TTAA 3 cut(s) 938, 957, 1239
SatI GCNGC 4 cut(s) 435, 827, 1050, 1346
Sau3AI GATC 5 cut(s) 112, 220, 1134, 1171, 1260
Sau96I GGNCC 1 cut(s) 1289
ScaI AGTACT 1 cut(s) 1341
SchI GAGTC 3 cut(s) 386, 593, 1040
ScrFI CCNGG 2 cut(s) 451, 572
SduI GDGCHC 1 cut(s) 341
SfcI CTRYAG 1 cut(s) 1343
SfuI TTCGAA 2 cut(s) 810, 1313
SinI GGWCC 1 cut(s) 1289
SmlI CTYRAG 1 cut(s) 850
SmoI CTYRAG 1 cut(s) 850
SphI GCATGC 1 cut(s) 1147
SseBI AGGCCT 1 cut(s) 279
SsiI CCGC 5 cut(s) 69, 434, 754, 878, 1303
SspI AATATT 2 cut(s) 153, 247
SspMI CTAG 5 cut(s) 47, 191, 1085, 1269, 1394
SstI GAGCTC 1 cut(s) 341
StuI AGGCCT 1 cut(s) 279
StyD4I CCNGG 2 cut(s) 449, 570
StyI CCWWGG 3 cut(s) 345, 480, 817
TaaI ACNGT 1 cut(s) 617
TaiI ACGT 2 cut(s) 474, 645
TaqI TCGA 7 cut(s) 639, 810, 965, 1006, 1174, 1190, 1313
TatI WGTACW 3 cut(s) 366, 946, 1339
TauI GCSGC 1 cut(s) 437
TfiI GAWTC 3 cut(s) 352, 1023, 1061
Tru1I TTAA 3 cut(s) 938, 957, 1239
Tru9I TTAA 3 cut(s) 938, 957, 1239
TscAI CASTG 3 cut(s) 319, 402, 523
TseI GCWGC 3 cut(s) 826, 1049, 1345
TspDTI ATGAA 9 cut(s) 95, 111, 147, 279, 339, 513, 762, 935, 1015
TspGWI ACGGA 3 cut(s) 776, 1208, 1396
TspRI CASTG 3 cut(s) 319, 402, 523
VpaK11BI GGWCC 1 cut(s) 1289
VspI ATTAAT 1 cut(s) 1239
XapI RAATTY 2 cut(s) 812, 883
XceI RCATGY 2 cut(s) 298, 1147
XmiI GTMKAC 1 cut(s) 1190
XmnI GAANNNNTTC 1 cut(s) 152
XspI CTAG 5 cut(s) 47, 191, 1085, 1269, 1394
ZraI GACGTC 1 cut(s) 643
ZrmI AGTACT 1 cut(s) 1341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.