Prupe.8G054000_v2.0.a1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0016258
Physical Location & Seq
Forward (+)
6129612 .. 6131018
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G054000.1

Sequence Viewer

Length: 1407 bp
ATGGAAGACATCGATGAGGAGGAGCTTTTGAATCTTAGCCTGGCAATTGTTACAACTTCAGGGGGTGAAAGAGTGAAGAAGAGGAAGAGAAGAGATCACAAAAATGTTTTGGTTAGTCCTCTAAATTCATATGAAGGTTGTGAAGGGAAGATATTCAGGCTGCTTCAAATGAGGGAACAAATGCTGAAGCTAGACCACAAGAAGAAAGGAGCTCATGTGCTTGATGAAGATGGAAAAGGCCTCCATTTGATCCACATGCTGCTCATAACAGCCACTGCGGTTAATGAAAACAATGTGAGCTCAGCCTTGGAGAATCTGAGTGAGTTGTACAAGAGTGTTTCCTTATGCGGCGACTCCGTTCAACGAGTAGTTGCTTATTTTGCAGATGGCTTGGCTGCAAGGCTTCTCACCCGAAAATCACCCTTTTTTGACATGATCATGAAGGAACCAACAAGTGATGAAGAGTTCATGGCGTTTACATCTCTCTACCGGGTTTCTCCATACTACCAGTTTGCTCATTTTACAGCTAACCAAGCAATCATTGAGGCATTTGAGAAGGAAGAAGAGAAGAACAATCGTGCGTTACATGTTATTGATTTTGATGTCTCGTATGGATTTCAATGGCCTTCTCTTATACAATCTCTCTCCGAAAAGGCAACCAGTGGCAATCGAATATCACTCAGAATAACTGGGTTTGGACAAAGCATGGATGAACTACGCGAGACAGAGAACAGACTGATAAGCTTCTCAAAGGGTTTTCGCAATCTTATTTTCGAATTCCAAGGGTTGTTGAGAGGCTCAAAGCTCATAAACTTGAGGAAGAAGAAAAACGAAACGATTGCAGTGAATTTGGTTTTTCATTTGAACACTTTGAGCAATAGTTTGAAGATATCTGACTCATTAAAATCTGTACATTTACTTAACCCTTCCATTGTAATCTTGGTTGAACAAGAAGGAAGCAGAAGCCCTAGAAGTTTCTTGTCAAGATTCATGGAGTCTTTGCATTACTTTGCTGCCATGTTTGATTCTTTAGATGATTGCCTACCTCTAGAGAGTACTGAAAGGTTGAGCATTGAGAAGAACCATCTTGGAAAAGAGATCAAATCCATGCTCAATTACGACATCGACGATGTAAATTGTTCGAAAATCGAAAAGATGGAAACATGGAAGGCTAGGATGGAGAGCCATGGTTTTGAAGGAATTAATCTAAGCTCCAAGTCCAAGATACAAGCAAGACTTCTTTTGAAAATCAGGACACATTATTGTCCCCTTCAGTTTGATGGAGAGAGCAGTGGTGGGAGTACTGCTGGGTTCAAGGTTTTTGAAAGAGATGATGGAAGAACAATATCTCTAGGGTGGCAAGACAGGTATCTGCTTACAGTTTCTGCATGGAACTGTGTACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

469

Amino Acids

53.32

Weight (kDa)

6.69

Isoelectric Point (pI)

40.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016258)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12280
malus_domestica MD05G1040300.v1.1 MD10G1046300.v1.1
prunus_persica Prupe.8G054000_v2.0.a1
pyrus_communis pycom05g03290
rosa_chinensis RchiOBHm_Chr6g0272211
rosa_laevigata RLG00000013690
rosa_multiflora Rmu_sc0005748.1_g000002
rosa_roxburghii Rroxscaffold_7G00196470
rosa_rugosa Rorug06G0067400
rosa_samantha Rh6BG187000 Rh6CG185100 Rh6DG176800
rosa_wichuraiana Rw0G003840 Rw6G015840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 720
AciI CCGC 2 cut(s) 278, 348
AclWI GGATC 1 cut(s) 244
AcsI RAATTY 3 cut(s) 124, 776, 847
AcuI CTGAAG 3 cut(s) 42, 206, 1256
AfaI GTAC 5 cut(s) 329, 912, 1057, 1303, 1401
AflIII ACRYGT 1 cut(s) 586
AjnI CCWGG 1 cut(s) 39
AluBI AGCT 8 cut(s) 25, 190, 212, 300, 527, 744, 805, 1212
AluI AGCT 8 cut(s) 25, 190, 212, 300, 527, 744, 805, 1212
Alw21I GWGCWC 2 cut(s) 214, 302
Alw26I GTCTC 2 cut(s) 610, 716
AlwI GGATC 1 cut(s) 244
AlwNI CAGNNNCTG 2 cut(s) 275, 1385
AoxI GGCC 2 cut(s) 238, 623
ApeKI GCWGC 4 cut(s) 160, 259, 395, 1013
ApoI RAATTY 3 cut(s) 124, 776, 847
AseI ATTAAT 1 cut(s) 1203
Asp700I GAANNNNTTC 1 cut(s) 152
AsuC2I CCSGG 1 cut(s) 491
AsuHPI GGTGA 3 cut(s) 77, 400, 411
AsuII TTCGAA 2 cut(s) 774, 1142
BanII GRGCYC 2 cut(s) 214, 302
BbsI GAAGAC 1 cut(s) 12
Bbv12I GWGCWC 2 cut(s) 214, 302
BbvI GCAGC 4 cut(s) 147, 246, 382, 1000
BccI CCATC 7 cut(s) 224, 380, 1092, 1150, 1171, 1274, 1328
BcgI CGANNNNNNTGC 2 cut(s) 821, 855
BciT130I CCWGG 1 cut(s) 41
BclI TGATCA 1 cut(s) 435
BcnI CCSGG 1 cut(s) 491
BcoDI GTCTC 2 cut(s) 610, 716
BfaI CTAG 5 cut(s) 191, 969, 1049, 1173, 1352
BisI GCNGC 5 cut(s) 161, 260, 349, 396, 1014
BlpI GCTNAGC 1 cut(s) 301
BlsI GCNGC 5 cut(s) 162, 261, 350, 397, 1015
BmcAI AGTACT 2 cut(s) 1057, 1303
Bme1390I CCNGG 2 cut(s) 41, 491
BmiI GGNNCC 1 cut(s) 447
BmrFI CCNGG 2 cut(s) 41, 491
BmrI ACTGGG 1 cut(s) 699
BmuI ACTGGG 1 cut(s) 699
BpiI GAAGAC 1 cut(s) 12
Bpu1102I GCTNAGC 1 cut(s) 301
Bpu14I TTCGAA 2 cut(s) 774, 1142
BpuEI CTTGAG 1 cut(s) 835
BpuMI CCSGG 1 cut(s) 491
Bsa29I ATCGAT 1 cut(s) 12
BsaJI CCNNGG 3 cut(s) 306, 781, 1186
BsaXI ACNNNNNCTCC 2 cut(s) 201, 231
Bse1I ACTGG 3 cut(s) 508, 660, 694
BseBI CCWGG 1 cut(s) 41
BseCI ATCGAT 1 cut(s) 12
BseDI CCNNGG 3 cut(s) 306, 781, 1186
BseGI GGATG 2 cut(s) 715, 1182
BseMII CTCAG 3 cut(s) 308, 315, 694
BseNI ACTGG 3 cut(s) 508, 660, 694
BseRI GAGGAG 2 cut(s) 32, 35
BseXI GCAGC 4 cut(s) 147, 246, 382, 1000
BseYI CCCAGC 1 cut(s) 1307
Bsh1236I CGCG 1 cut(s) 720
BshFI GGCC 2 cut(s) 240, 625
BshVI ATCGAT 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 214, 302
BsiSI CCGG 1 cut(s) 490
BslFI GGGAC 1 cut(s) 1251
BsmAI GTCTC 2 cut(s) 610, 716
BsmFI GGGAC 1 cut(s) 1251
BsnI GGCC 2 cut(s) 240, 625
Bsp119I TTCGAA 2 cut(s) 774, 1142
Bsp1286I GDGCHC 2 cut(s) 214, 302
Bsp1407I TGTACA 2 cut(s) 327, 910
Bsp143I GATC 4 cut(s) 94, 249, 435, 1098
Bsp1720I GCTNAGC 1 cut(s) 301
Bsp19I CCATGG 1 cut(s) 1186
BspACI CCGC 2 cut(s) 278, 348
BspANI GGCC 2 cut(s) 240, 625
BspCNI CTCAG 3 cut(s) 309, 314, 693
BspDI ATCGAT 1 cut(s) 12
BspFNI CGCG 1 cut(s) 720
BspHI TCATGA 1 cut(s) 438
BspLI GGNNCC 1 cut(s) 447
BspPI GGATC 1 cut(s) 244
BspT104I TTCGAA 2 cut(s) 774, 1142
BsrGI TGTACA 2 cut(s) 327, 910
BsrI ACTGG 3 cut(s) 508, 660, 694
BssECI CCNNGG 3 cut(s) 306, 781, 1186
BssMI GATC 4 cut(s) 94, 249, 435, 1098
BssT1I CCWWGG 3 cut(s) 306, 781, 1186
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 2 cut(s) 1381, 1397
Bst6I CTCTTC 5 cut(s) 74, 80, 85, 456, 558
BstAUI TGTACA 2 cut(s) 327, 910
BstBI TTCGAA 2 cut(s) 774, 1142
BstDEI CTNAG 5 cut(s) 35, 301, 317, 680, 1208
BstDSI CCRYGG 1 cut(s) 1186
BstF5I GGATG 2 cut(s) 715, 1182
BstFNI CGCG 1 cut(s) 720
BstKTI GATC 4 cut(s) 97, 252, 438, 1101
BstMAI GTCTC 2 cut(s) 610, 716
BstMBI GATC 4 cut(s) 94, 249, 435, 1098
BstMWI GCNNNNNNNGC 2 cut(s) 380, 533
BstNI CCWGG 1 cut(s) 41
BstNSI RCATGY 2 cut(s) 259, 590
BstSCI CCNGG 2 cut(s) 39, 489
BstUI CGCG 1 cut(s) 720
BstV1I GCAGC 4 cut(s) 147, 246, 382, 1000
BstV2I GAAGAC 1 cut(s) 12
Bsu15I ATCGAT 1 cut(s) 12
BsuRI GGCC 2 cut(s) 240, 625
BsuTUI ATCGAT 1 cut(s) 12
BtgI CCRYGG 1 cut(s) 1186
BtsCI GGATG 2 cut(s) 715, 1182
BtsI GCAGTG 3 cut(s) 273, 849, 1297
BtsIMutI CAGTG 4 cut(s) 273, 667, 849, 1297
CaiI CAGNNNCTG 2 cut(s) 275, 1385
CciI TCATGA 1 cut(s) 438
ClaI ATCGAT 1 cut(s) 12
Csp6I GTAC 5 cut(s) 328, 911, 1056, 1302, 1400
CviQI GTAC 5 cut(s) 328, 911, 1056, 1302, 1400
DdeI CTNAG 5 cut(s) 35, 301, 317, 680, 1208
DpnI GATC 4 cut(s) 96, 251, 437, 1100
DpnII GATC 4 cut(s) 94, 249, 435, 1098
Eam1104I CTCTTC 5 cut(s) 74, 80, 85, 456, 558
EarI CTCTTC 5 cut(s) 74, 80, 85, 456, 558
Ecl136II GAGCTC 2 cut(s) 212, 300
Eco130I CCWWGG 3 cut(s) 306, 781, 1186
Eco147I AGGCCT 1 cut(s) 240
Eco24I GRGCYC 2 cut(s) 214, 302
Eco32I GATATC 1 cut(s) 891
Eco53kI GAGCTC 2 cut(s) 212, 300
Eco57I CTGAAG 3 cut(s) 42, 206, 1256
EcoICRI GAGCTC 2 cut(s) 212, 300
EcoRI GAATTC 1 cut(s) 776
EcoRII CCWGG 1 cut(s) 39
EcoRV GATATC 1 cut(s) 891
EcoT14I CCWWGG 3 cut(s) 306, 781, 1186
EcoT38I GRGCYC 2 cut(s) 214, 302
ErhI CCWWGG 3 cut(s) 306, 781, 1186
FalI AAGNNNNNCTT 2 cut(s) 1221, 1253
FaqI GGGAC 1 cut(s) 1251
FauNDI CATATG 1 cut(s) 130
FbaI TGATCA 1 cut(s) 435
Fnu4HI GCNGC 5 cut(s) 161, 260, 349, 396, 1014
FokI GGATG 2 cut(s) 722, 1189
FriOI GRGCYC 2 cut(s) 214, 302
Fsp4HI GCNGC 5 cut(s) 161, 260, 349, 396, 1014
FspBI CTAG 5 cut(s) 191, 969, 1049, 1173, 1352
GluI GCNGC 5 cut(s) 161, 260, 349, 396, 1014
GsaI CCCAGC 1 cut(s) 1311
HaeIII GGCC 2 cut(s) 240, 625
HapII CCGG 1 cut(s) 490
HindIII AAGCTT 1 cut(s) 742
HinfI GANTC 7 cut(s) 31, 313, 353, 896, 987, 995, 1025
HpaII CCGG 1 cut(s) 490
HphI GGTGA 3 cut(s) 77, 400, 411
Hpy166II GTNNAC 2 cut(s) 477, 1400
Hpy188I TCNGA 4 cut(s) 318, 649, 683, 895
Hpy188III TCNNGA 4 cut(s) 439, 984, 1049, 1252
Hpy8I GTNNAC 2 cut(s) 477, 1400
Hpy99I CGWCG 1 cut(s) 1130
HpyCH4III ACNGT 2 cut(s) 1381, 1397
HpyCH4V TGCA 5 cut(s) 383, 398, 842, 1003, 1388
HpyF10VI GCNNNNNNNGC 2 cut(s) 380, 533
HpyF3I CTNAG 5 cut(s) 35, 301, 317, 680, 1208
Ksp22I TGATCA 1 cut(s) 435
Kzo9I GATC 4 cut(s) 94, 249, 435, 1098
LmnI GCTCC 3 cut(s) 22, 209, 1217
Lsp1109I GCAGC 4 cut(s) 147, 246, 382, 1000
MaeI CTAG 5 cut(s) 191, 969, 1049, 1173, 1352
MaeIII GTNAC 2 cut(s) 49, 582
MalI GATC 4 cut(s) 96, 251, 437, 1100
MboI GATC 4 cut(s) 94, 249, 435, 1098
MfeI CAATTG 1 cut(s) 45
MhlI GDGCHC 2 cut(s) 214, 302
MluCI AATT 7 cut(s) 45, 124, 776, 847, 1114, 1135, 1200
MlyI GAGTC 3 cut(s) 347, 890, 1004
MroXI GAANNNNTTC 1 cut(s) 152
MseI TTAA 4 cut(s) 282, 902, 921, 1203
MslI CAYNNNNRTG 2 cut(s) 102, 437
MspI CCGG 1 cut(s) 490
MspR9I CCNGG 2 cut(s) 41, 491
MunI CAATTG 1 cut(s) 45
MvaI CCWGG 1 cut(s) 41
MvnI CGCG 1 cut(s) 720
MwoI GCNNNNNNNGC 2 cut(s) 380, 533
NciI CCSGG 1 cut(s) 491
NcoI CCATGG 1 cut(s) 1186
NdeI CATATG 1 cut(s) 130
NdeII GATC 4 cut(s) 94, 249, 435, 1098
NlaIV GGNNCC 1 cut(s) 447
NspI RCATGY 2 cut(s) 259, 590
NspV TTCGAA 2 cut(s) 774, 1142
PagI TCATGA 1 cut(s) 438
PceI AGGCCT 1 cut(s) 240
PciI ACATGT 1 cut(s) 586
PcsI WCGNNNNNNNCGW 1 cut(s) 1125
PdmI GAANNNNTTC 1 cut(s) 152
PfeI GAWTC 4 cut(s) 31, 313, 987, 1025
PkrI GCNGC 5 cut(s) 162, 261, 350, 397, 1015
PleI GAGTC 3 cut(s) 347, 890, 1003
PpsI GAGTC 3 cut(s) 347, 890, 1003
PscI ACATGT 1 cut(s) 586
PshBI ATTAAT 1 cut(s) 1203
Psp124BI GAGCTC 2 cut(s) 214, 302
Psp6I CCWGG 1 cut(s) 39
PspFI CCCAGC 1 cut(s) 1307
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 1 cut(s) 447
PstNI CAGNNNCTG 2 cut(s) 275, 1385
RsaI GTAC 5 cut(s) 329, 912, 1057, 1303, 1401
RsaNI GTAC 5 cut(s) 328, 911, 1056, 1302, 1400
RseI CAYNNNNRTG 2 cut(s) 102, 437
SacI GAGCTC 2 cut(s) 214, 302
SaqAI TTAA 4 cut(s) 282, 902, 921, 1203
SatI GCNGC 5 cut(s) 161, 260, 349, 396, 1014
Sau3AI GATC 4 cut(s) 94, 249, 435, 1098
ScaI AGTACT 2 cut(s) 1057, 1303
SchI GAGTC 3 cut(s) 347, 890, 1004
ScrFI CCNGG 2 cut(s) 41, 491
SduI GDGCHC 2 cut(s) 214, 302
SfuI TTCGAA 2 cut(s) 774, 1142
SmiMI CAYNNNNRTG 2 cut(s) 102, 437
SmlI CTYRAG 1 cut(s) 814
SmoI CTYRAG 1 cut(s) 814
Sse9I AATT 7 cut(s) 45, 124, 776, 847, 1114, 1135, 1200
SseBI AGGCCT 1 cut(s) 240
SsiI CCGC 2 cut(s) 278, 348
SspMI CTAG 5 cut(s) 191, 969, 1049, 1173, 1352
SstI GAGCTC 2 cut(s) 214, 302
StuI AGGCCT 1 cut(s) 240
StyD4I CCNGG 2 cut(s) 39, 489
StyI CCWWGG 3 cut(s) 306, 781, 1186
TaaI ACNGT 2 cut(s) 1381, 1397
TaqI TCGA 6 cut(s) 12, 670, 774, 1125, 1142, 1149
TasI AATT 7 cut(s) 45, 124, 776, 847, 1114, 1135, 1200
TatI WGTACW 4 cut(s) 327, 910, 1055, 1301
TauI GCSGC 1 cut(s) 351
TfiI GAWTC 4 cut(s) 31, 313, 987, 1025
Tru1I TTAA 4 cut(s) 282, 902, 921, 1203
Tru9I TTAA 4 cut(s) 282, 902, 921, 1203
TscAI CASTG 4 cut(s) 280, 667, 849, 1297
TseI GCWGC 4 cut(s) 160, 259, 395, 1013
TspGWI ACGGA 1 cut(s) 346
TspRI CASTG 4 cut(s) 280, 667, 849, 1297
VspI ATTAAT 1 cut(s) 1203
XapI RAATTY 3 cut(s) 124, 776, 847
XbaI TCTAGA 1 cut(s) 1048
XceI RCATGY 2 cut(s) 259, 590
XcmI CCANNNNNNNNNTGG 1 cut(s) 937
XmnI GAANNNNTTC 1 cut(s) 152
XspI CTAG 5 cut(s) 191, 969, 1049, 1173, 1352
ZrmI AGTACT 2 cut(s) 1057, 1303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.