MD10G1046300.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016258
Physical Location & Seq
Forward (+)
6180700 .. 6182115
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1046300.v1.1.491

Sequence Viewer

Length: 1416 bp
ATGGAAGACATAGATGAGGAGGAGGAGCTTTTGAATCTTAGCCTTGCGATTGTTACAAGTACAGGGAGGGAAAGATTTAGAAAAAGGAAGAGATCAAGAGATGATGGTGTTGCTAATGGTTCGGTGAGCCCTCTAAACTCTTATGAAGGTTGTGAAGGGAAGATATTCAGGCTTCTTCAAATGAGGGAACAAATGCTAAAAAACCTAGACCACCACAAGAAGAAAGGGGTTGATGTCGATGATGAAGACGGAAAAGGACTGCACCTGATCCACATGTTGCTCATAACAGCCACTGCAGTCAACGAAAACAATGTAGGCTCAGCCTTGGAGCATCTCACTGAGTTGTACAAGAGTGTTTCATTATCCGGTGACTCCATCCAACGAGTAGTGGCTTATTTCGCTGACAGCTTGGCCGCAAGGCTTCTCACACGAAGATCCCCCTTTTATGACATCATCATGAAGGAACCGACGAGCGAAGAAGAGTTTGTAGCGTTCACCGCTCTCTATCGGGTGTCTCCGTACTACCAATTTGCTCATTTCACGGCAATCCAAGCAATCATTGAGGCCTTTGAGAAGGAAGAAGAGAAGAACAATCGTGCATTACATGTTATCGATTTCGATGTCTCCTATGGATTTCAGTGGGCATCTCTTATACAGTCTCTCTCCGAGAAGGCAAGCAGTAGGAAGCGAATATCGCTCAGAATAACTGGGTTTGGAAGAAGCGTGGATGAGCTACGGGAAACGGAGAACAGACTGACAACCTTCTCAAATGGGTTTCAGAATCTTGCTTTTGAATTTCAAGGGTTGTTGAGAGGCTCAAAGCTCGTAAACTTGAGGAAAAAGAGGAACGAAACACTGGCAGTGAATTTGGTTTTTCATTTGAATACTTTGAGCAATAATTTGAAGATATCTGACTTGTTAAAATCTGTACATTTACTTAACCCTTCCATTGTAATCTTGGTTGAACAAGAAGGGACCAGGAGTCCTCGAAGATTCTTATCGAGATTCATGGAGTCTTTGCATTATTTTGCAGCCATGTTTGATTCTTTAGATGAATGTCTGCCATTAGAGAGTGCTGAAAGGTTGAGCATTGAGAAGAACCACCTTGGAAAAGAGATCAAAAGCATGCTCAATTACGACAATAACAACGATGATAATTGTCTGAAAATTGAAAAGATGGAAACATGGAAGGCGAGGATGGAGAGTCATGGTTTTGAAGGAATAAATCTAAGCTCCAGGTCCAAGATACAAGCAAGACTTCTTTTGAAAATAAGGACTCACTATTGTTCACTTGATTTTGATGGAGAGAGTAATGGAGCTGCAGCTGGCTTCAAGGTTTTCGACAGAGATGATGGAAGGACGATATCTCTTGGGTGGCAAGACAGGTATCTGCTTACAGTTTCCGCATGGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

472

Amino Acids

53.93

Weight (kDa)

6.31

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 90 - 470 8e-88 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016258)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12280
malus_domestica MD05G1040300.v1.1 MD10G1046300.v1.1
prunus_persica Prupe.8G054000_v2.0.a1
pyrus_communis pycom05g03290
rosa_chinensis RchiOBHm_Chr6g0272211
rosa_laevigata RLG00000013690
rosa_multiflora Rmu_sc0005748.1_g000002
rosa_roxburghii Rroxscaffold_7G00196470
rosa_rugosa Rorug06G0067400
rosa_samantha Rh6BG187000 Rh6CG185100 Rh6DG176800
rosa_wichuraiana Rw0G003840 Rw6G015840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 500
AciI CCGC 3 cut(s) 414, 498, 1404
AclWI GGATC 2 cut(s) 262, 429
AcoI YGGCCR 1 cut(s) 411
AcsI RAATTY 2 cut(s) 794, 865
AfaI GTAC 4 cut(s) 61, 347, 521, 930
AflIII ACRYGT 2 cut(s) 273, 604
AhdI GACNNNNNGTC 1 cut(s) 981
AjnI CCWGG 2 cut(s) 977, 1235
AluBI AGCT 7 cut(s) 28, 408, 733, 823, 1233, 1319, 1325
AluI AGCT 7 cut(s) 28, 408, 733, 823, 1233, 1319, 1325
Alw26I GTCTC 3 cut(s) 519, 628, 663
AlwI GGATC 2 cut(s) 262, 429
AlwNI CAGNNNCTG 1 cut(s) 293
AoxI GGCC 2 cut(s) 411, 564
ApeKI GCWGC 3 cut(s) 1031, 1319, 1322
ApoI RAATTY 2 cut(s) 794, 865
Asp700I GAANNNNTTC 1 cut(s) 164
AspS9I GGNCC 2 cut(s) 975, 1239
AsuHPI GGTGA 3 cut(s) 136, 380, 487
AvaII GGWCC 2 cut(s) 975, 1239
BanII GRGCYC 1 cut(s) 131
BbsI GAAGAC 2 cut(s) 12, 252
BbvI GCAGC 3 cut(s) 1043, 1306, 1334
BccI CCATC 6 cut(s) 98, 383, 1171, 1192, 1295, 1346
BceAI ACGGC 1 cut(s) 558
BciT130I CCWGG 2 cut(s) 979, 1237
BcoDI GTCTC 3 cut(s) 519, 628, 663
BfaI CTAG 1 cut(s) 206
BfmI CTRYAG 2 cut(s) 294, 1320
BisI GCNGC 4 cut(s) 414, 1032, 1320, 1323
BlpI GCTNAGC 1 cut(s) 319
BlsI GCNGC 4 cut(s) 415, 1033, 1321, 1324
Bme1390I CCNGG 2 cut(s) 979, 1237
Bme18I GGWCC 2 cut(s) 975, 1239
BmeRI GACNNNNNGTC 1 cut(s) 981
BmgT120I GGNCC 2 cut(s) 975, 1239
BmiI GGNNCC 2 cut(s) 465, 976
BmrFI CCNGG 2 cut(s) 979, 1237
BmrI ACTGGG 1 cut(s) 717
BmsI GCATC 2 cut(s) 340, 653
BmuI ACTGGG 1 cut(s) 717
BpiI GAAGAC 2 cut(s) 12, 252
BpmI CTGGAG 1 cut(s) 1219
Bpu1102I GCTNAGC 1 cut(s) 319
BpuEI CTTGAG 1 cut(s) 853
Bsa29I ATCGAT 1 cut(s) 612
BsaBI GATNNNNATC 1 cut(s) 997
BsaJI CCNNGG 2 cut(s) 324, 1105
BsaWI WCCGGW 1 cut(s) 365
Bse1I ACTGG 2 cut(s) 712, 861
Bse8I GATNNNNATC 1 cut(s) 997
BseBI CCWGG 2 cut(s) 979, 1237
BseCI ATCGAT 1 cut(s) 612
BseDI CCNNGG 2 cut(s) 324, 1105
BseGI GGATG 3 cut(s) 375, 733, 1203
BseJI GATNNNNATC 1 cut(s) 997
BseMII CTCAG 3 cut(s) 330, 333, 712
BseNI ACTGG 2 cut(s) 712, 861
BseRI GAGGAG 3 cut(s) 32, 35, 38
BseXI GCAGC 3 cut(s) 1043, 1306, 1334
BsgI GTGCAG 1 cut(s) 245
BshFI GGCC 2 cut(s) 413, 566
BshVI ATCGAT 1 cut(s) 612
BsiSI CCGG 1 cut(s) 366
BslFI GGGAC 1 cut(s) 988
BsmAI GTCTC 3 cut(s) 519, 628, 663
BsmFI GGGAC 1 cut(s) 988
BsnI GGCC 2 cut(s) 413, 566
Bsp1286I GDGCHC 1 cut(s) 131
Bsp1407I TGTACA 2 cut(s) 345, 928
Bsp143I GATC 4 cut(s) 92, 267, 434, 1116
Bsp1720I GCTNAGC 1 cut(s) 319
BspACI CCGC 3 cut(s) 414, 498, 1404
BspANI GGCC 2 cut(s) 413, 566
BspCNI CTCAG 3 cut(s) 331, 332, 711
BspDI ATCGAT 1 cut(s) 612
BspHI TCATGA 1 cut(s) 456
BspLI GGNNCC 2 cut(s) 465, 976
BspMAI CTGCAG 2 cut(s) 298, 1324
BspPI GGATC 2 cut(s) 262, 429
BsrBI CCGCTC 1 cut(s) 500
BsrGI TGTACA 2 cut(s) 345, 928
BsrI ACTGG 2 cut(s) 712, 861
BssECI CCNNGG 2 cut(s) 324, 1105
BssMI GATC 4 cut(s) 92, 267, 434, 1116
BssT1I CCWWGG 2 cut(s) 324, 1105
Bst2UI CCWGG 2 cut(s) 979, 1237
Bst4CI ACNGT 2 cut(s) 657, 1399
Bst6I CTCTTC 3 cut(s) 83, 474, 576
BstAUI TGTACA 2 cut(s) 345, 928
BstC8I GCNNGC 3 cut(s) 676, 1127, 1327
BstDEI CTNAG 5 cut(s) 38, 319, 339, 698, 1229
BstF5I GGATG 3 cut(s) 375, 733, 1203
BstKTI GATC 4 cut(s) 95, 270, 437, 1119
BstMAI GTCTC 3 cut(s) 519, 628, 663
BstMBI GATC 4 cut(s) 92, 267, 434, 1116
BstMWI GCNNNNNNNGC 4 cut(s) 398, 497, 551, 694
BstNI CCWGG 2 cut(s) 979, 1237
BstNSI RCATGY 3 cut(s) 277, 608, 1129
BstSCI CCNGG 2 cut(s) 977, 1235
BstSFI CTRYAG 2 cut(s) 294, 1320
BstV1I GCAGC 3 cut(s) 1043, 1306, 1334
BstV2I GAAGAC 2 cut(s) 12, 252
BstX2I RGATCY 1 cut(s) 434
BstYI RGATCY 1 cut(s) 434
Bsu15I ATCGAT 1 cut(s) 612
BsuRI GGCC 2 cut(s) 413, 566
BsuTUI ATCGAT 1 cut(s) 612
BtsCI GGATG 3 cut(s) 375, 733, 1203
BtsI GCAGTG 2 cut(s) 291, 867
BtsIMutI CAGTG 6 cut(s) 291, 336, 644, 854, 867, 1411
Cac8I GCNNGC 3 cut(s) 676, 1127, 1327
CaiI CAGNNNCTG 1 cut(s) 293
CciI TCATGA 1 cut(s) 456
Cfr13I GGNCC 2 cut(s) 975, 1239
ClaI ATCGAT 1 cut(s) 612
Csp6I GTAC 4 cut(s) 60, 346, 520, 929
CviAII CATG 9 cut(s) 274, 457, 605, 1009, 1036, 1126, 1185, 1208, 1407
CviQI GTAC 4 cut(s) 60, 346, 520, 929
DdeI CTNAG 5 cut(s) 38, 319, 339, 698, 1229
DpnI GATC 4 cut(s) 94, 269, 436, 1118
DpnII GATC 4 cut(s) 92, 267, 434, 1116
DriI GACNNNNNGTC 1 cut(s) 981
EaeI YGGCCR 1 cut(s) 411
Eam1104I CTCTTC 3 cut(s) 83, 474, 576
Eam1105I GACNNNNNGTC 1 cut(s) 981
EarI CTCTTC 3 cut(s) 83, 474, 576
Eco130I CCWWGG 2 cut(s) 324, 1105
Eco147I AGGCCT 1 cut(s) 566
Eco24I GRGCYC 1 cut(s) 131
Eco32I GATATC 2 cut(s) 909, 1365
Eco47I GGWCC 2 cut(s) 975, 1239
EcoRII CCWGG 2 cut(s) 977, 1235
EcoRV GATATC 2 cut(s) 909, 1365
EcoT14I CCWWGG 2 cut(s) 324, 1105
EcoT38I GRGCYC 1 cut(s) 131
ErhI CCWWGG 2 cut(s) 324, 1105
FaeI CATG 9 cut(s) 277, 460, 608, 1012, 1039, 1129, 1188, 1211, 1410
FalI AAGNNNNNCTT 2 cut(s) 1242, 1274
FaqI GGGAC 1 cut(s) 988
FatI CATG 9 cut(s) 273, 456, 604, 1008, 1035, 1125, 1184, 1207, 1406
Fnu4HI GCNGC 4 cut(s) 414, 1032, 1320, 1323
FokI GGATG 3 cut(s) 362, 740, 1210
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 4 cut(s) 414, 1032, 1320, 1323
FspBI CTAG 1 cut(s) 206
GluI GCNGC 4 cut(s) 414, 1032, 1320, 1323
GsuI CTGGAG 1 cut(s) 1219
HaeIII GGCC 2 cut(s) 413, 566
HapII CCGG 1 cut(s) 366
Hin1II CATG 9 cut(s) 277, 460, 608, 1012, 1039, 1129, 1188, 1211, 1410
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HpaII CCGG 1 cut(s) 366
HphI GGTGA 3 cut(s) 136, 380, 487
Hpy166II GTNNAC 4 cut(s) 301, 495, 829, 1289
Hpy188I TCNGA 5 cut(s) 667, 701, 780, 913, 1164
Hpy188III TCNNGA 3 cut(s) 96, 457, 1002
Hpy8I GTNNAC 4 cut(s) 301, 495, 829, 1289
Hpy99I CGWCG 1 cut(s) 472
HpyCH4III ACNGT 2 cut(s) 657, 1399
HpyCH4V TGCA 6 cut(s) 262, 296, 599, 1021, 1031, 1322
HpyF10VI GCNNNNNNNGC 4 cut(s) 398, 497, 551, 694
HpyF3I CTNAG 5 cut(s) 38, 319, 339, 698, 1229
Hsp92II CATG 9 cut(s) 277, 460, 608, 1012, 1039, 1129, 1188, 1211, 1410
Kzo9I GATC 4 cut(s) 92, 267, 434, 1116
LmnI GCTCC 4 cut(s) 25, 328, 1238, 1316
Lsp1109I GCAGC 3 cut(s) 1043, 1306, 1334
LweI GCATC 2 cut(s) 340, 653
MaeI CTAG 1 cut(s) 206
MaeIII GTNAC 2 cut(s) 52, 368
MalI GATC 4 cut(s) 94, 269, 436, 1118
MbiI CCGCTC 1 cut(s) 500
MboI GATC 4 cut(s) 92, 267, 434, 1116
MflI RGATCY 1 cut(s) 434
MhlI GDGCHC 1 cut(s) 131
MluCI AATT 7 cut(s) 527, 794, 865, 898, 1132, 1156, 1167
MlyI GAGTC 5 cut(s) 365, 991, 1022, 1213, 1270
MmeI TCCRAC 1 cut(s) 403
MroXI GAANNNNTTC 1 cut(s) 164
MseI TTAA 2 cut(s) 920, 939
MslI CAYNNNNRTG 1 cut(s) 455
MspA1I CMGCKG 1 cut(s) 1325
MspI CCGG 1 cut(s) 366
MspR9I CCNGG 2 cut(s) 979, 1237
MvaI CCWGG 2 cut(s) 979, 1237
MwoI GCNNNNNNNGC 4 cut(s) 398, 497, 551, 694
NdeII GATC 4 cut(s) 92, 267, 434, 1116
NlaIII CATG 9 cut(s) 277, 460, 608, 1012, 1039, 1129, 1188, 1211, 1410
NlaIV GGNNCC 2 cut(s) 465, 976
NmuCI GTSAC 1 cut(s) 368
NspI RCATGY 3 cut(s) 277, 608, 1129
PaeI GCATGC 1 cut(s) 1129
PagI TCATGA 1 cut(s) 456
PceI AGGCCT 1 cut(s) 566
PciI ACATGT 2 cut(s) 273, 604
PdmI GAANNNNTTC 1 cut(s) 164
PfeI GAWTC 5 cut(s) 34, 781, 993, 1005, 1043
PkrI GCNGC 4 cut(s) 415, 1033, 1321, 1324
PleI GAGTC 5 cut(s) 365, 990, 1021, 1212, 1270
PpsI GAGTC 5 cut(s) 365, 990, 1021, 1212, 1270
PscI ACATGT 2 cut(s) 273, 604
Psp6I CCWGG 2 cut(s) 977, 1235
PspGI CCWGG 2 cut(s) 977, 1235
PspN4I GGNNCC 2 cut(s) 465, 976
PspPI GGNCC 2 cut(s) 975, 1239
PstI CTGCAG 2 cut(s) 298, 1324
PstNI CAGNNNCTG 1 cut(s) 293
PsuI RGATCY 1 cut(s) 434
PvuII CAGCTG 1 cut(s) 1325
RsaI GTAC 4 cut(s) 61, 347, 521, 930
RsaNI GTAC 4 cut(s) 60, 346, 520, 929
RseI CAYNNNNRTG 1 cut(s) 455
SaqAI TTAA 2 cut(s) 920, 939
SatI GCNGC 4 cut(s) 414, 1032, 1320, 1323
Sau3AI GATC 4 cut(s) 92, 267, 434, 1116
Sau96I GGNCC 2 cut(s) 975, 1239
SchI GAGTC 5 cut(s) 365, 991, 1022, 1213, 1270
ScrFI CCNGG 2 cut(s) 979, 1237
SduI GDGCHC 1 cut(s) 131
SfaNI GCATC 2 cut(s) 340, 653
SfcI CTRYAG 2 cut(s) 294, 1320
SinI GGWCC 2 cut(s) 975, 1239
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 1 cut(s) 832
SmoI CTYRAG 1 cut(s) 832
SphI GCATGC 1 cut(s) 1129
Sse9I AATT 7 cut(s) 527, 794, 865, 898, 1132, 1156, 1167
SseBI AGGCCT 1 cut(s) 566
SsiI CCGC 3 cut(s) 414, 498, 1404
SspMI CTAG 1 cut(s) 206
StuI AGGCCT 1 cut(s) 566
StyD4I CCNGG 2 cut(s) 977, 1235
StyI CCWWGG 2 cut(s) 324, 1105
TaaI ACNGT 2 cut(s) 657, 1399
TaqI TCGA 6 cut(s) 237, 612, 618, 988, 1001, 1341
TasI AATT 7 cut(s) 527, 794, 865, 898, 1132, 1156, 1167
TatI WGTACW 3 cut(s) 59, 345, 928
TauI GCSGC 1 cut(s) 416
TfiI GAWTC 5 cut(s) 34, 781, 993, 1005, 1043
Tru1I TTAA 2 cut(s) 920, 939
Tru9I TTAA 2 cut(s) 920, 939
TscAI CASTG 5 cut(s) 298, 343, 644, 861, 867
TseFI GTSAC 1 cut(s) 368
TseI GCWGC 3 cut(s) 1031, 1319, 1322
Tsp45I GTSAC 1 cut(s) 368
TspDTI ATGAA 7 cut(s) 159, 258, 348, 473, 866, 997, 1068
TspGWI ACGGA 3 cut(s) 264, 507, 758
TspRI CASTG 5 cut(s) 298, 343, 644, 861, 867
VpaK11BI GGWCC 2 cut(s) 975, 1239
XapI RAATTY 2 cut(s) 794, 865
XceI RCATGY 3 cut(s) 277, 608, 1129
XcmI CCANNNNNNNNNTGG 1 cut(s) 955
XmnI GAANNNNTTC 1 cut(s) 164
XspI CTAG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.