FvH4_2g12420

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014655
Physical Location & Seq
Reverse (-)
10896514 .. 10900471
3958 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g12420.t1

Sequence Viewer

Length: 1179 bp
ATGGCTGGAAACTCATCATGTCTGAGGCTTTTCATCTTAGGTGTCTCCCTCCTCTCTTGCTCGTTCCAATGCTTCTGTCTTTGTAATGTAAACAGAAAAGGTGAAACTAGAAGCACTACTGCTGGGATCAAAGGGATGTTTGTGTTCGGAAGCTCTCTGGTTGACAATGGCAACAACAACTTCCTACAAAACTCACTGGCCAGAGCTGATTACTGGCCATATGGCGTTGATTTTCCTCTTGGGCCTTCTGGGAGATTCACAAATGGAAAGAATGTCATCGATCTTCTTGGAGAGCAGCTTAAGATACCTTCCTTGATTCCAGTATTCGCTGATCCCCAAACTAAAGGAAGCAAAATTGTTCATGGTGTCAACTTCGCTTCTGGTGCTTCTGGTATACTCGATGATACTGGCTCCTTAGCGGGACATGTAATGAGTCTGAACGAGCAAATAAGAAATTTCGAGGAGGTAACTTTGCCAGAGTTGAAAACCCAGTTAGGGTTTGGGATGGGGTGCAGTAAAAGCTTGGGATCATCAGTTGGTAACTACTTGTTTGTGGTGGGAACTGGTGGTAACGATTACTCTTTCAACTACTTCCTGGCCAACCCCAATAATGTCAGTCTTCAAGTCTTCACCGCCAATCTCACTTCATCTCTCTCCACACAACTAAAGAAGTTGCACAGTTTGGGAGCAAGAAAATTTGTGGTGATGTCAATAAACCCACTCGGGTGCAGTCCGATGGCTATCAACATGAAGAAGCTGCTGCCAACCGTGATGGGGCGTAATCGTTGCGTACAAGATATGAACCGGGCAGCTCACCTTTTCAATTCTCAGTTCAAGTCTATGGTTGATGCCATACGAGCTCAAATGCCCGGTTCAGCACTTGTATTCGTCAACTCCTACAAGATTATCAGGGACATAATCAAGAATCCCATCTCCAAAGGATTCAGAGATTTGAGAGGGACGTGTTGTGAAGTGGCAACAATAAGTAACGGAGGAAATGGAATCCTATGCAAAAGGGGAGGGCAAGTGTGCCTGAATAGAAGCAGTTATGTCTTCTTTGATGGCTTGCATCCAACTGAAGCAGTGAACGTTCAAATAGCAACCAAGGCATTCGTCTCCACTCTCAAAACTGAAGTTTATCCAACCAATATCCAACAGTTGATCCAAATCCAAATATAG

Protein Analysis

393

Amino Acids

42.42

Weight (kDa)

9.32

Isoelectric Point (pI)

22.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 47 - 367 3.3e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014655)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12420
malus_domestica MD10G1044500.v1.1
prunus_persica Prupe.7G048300_v2.0.a1 Prupe.8G051400_v2.0.a1 Prupe.8G051400_v2.0.a1
pyrus_communis pycom10g03120
rosa_chinensis RchiOBHm_Chr6g0272561
rosa_laevigata RLG00000013666
rosa_multiflora Rmu_co8481641.1_g000001 Rmu_sc0003080.1_g000014
rosa_roxburghii Rroxscaffold_7G00196120
rosa_rugosa Rorug06G0070100
rosa_samantha Rh6AG186400 Rh6BG189500 Rh6CG187300 Rh6DG179000
rosa_wichuraiana Rw6G016060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 394
AciI CCGC 2 cut(s) 419, 633
AclI AACGTT 1 cut(s) 1089
AclWI GGATC 4 cut(s) 134, 326, 535, 1156
AcoI YGGCCR 3 cut(s) 198, 215, 597
AcsI RAATTY 2 cut(s) 454, 695
AcuI CTGAAG 2 cut(s) 1098, 1152
AfaI GTAC 1 cut(s) 792
AfiI CCNNNNNNNGG 2 cut(s) 495, 774
AflII CTTAAG 1 cut(s) 299
AflIII ACRYGT 2 cut(s) 424, 962
AgsI TTSAA 6 cut(s) 484, 586, 623, 823, 835, 1094
AjiI CACGTC 1 cut(s) 963
AjnI CCWGG 1 cut(s) 594
AleI CACNNNNGTG 1 cut(s) 724
AluBI AGCT 7 cut(s) 153, 206, 298, 522, 757, 812, 860
AluI AGCT 7 cut(s) 153, 206, 298, 522, 757, 812, 860
Alw21I GWGCWC 1 cut(s) 862
Alw26I GTCTC 2 cut(s) 49, 1120
AlwI GGATC 4 cut(s) 134, 326, 535, 1156
Ama87I CYCGRG 1 cut(s) 722
AoxI GGCC 4 cut(s) 198, 215, 242, 597
ApeKI GCWGC 4 cut(s) 295, 757, 760, 809
ApoI RAATTY 2 cut(s) 454, 695
AspS9I GGNCC 1 cut(s) 242
AsuC2I CCSGG 2 cut(s) 806, 870
AsuHPI GGTGA 4 cut(s) 113, 622, 715, 806
AvaI CYCGRG 1 cut(s) 722
BalI TGGCCA 3 cut(s) 200, 217, 599
BanII GRGCYC 1 cut(s) 862
BbsI GAAGAC 3 cut(s) 611, 619, 1045
Bbv12I GWGCWC 1 cut(s) 862
BbvI GCAGC 4 cut(s) 307, 744, 747, 821
BccI CCATC 5 cut(s) 499, 730, 766, 938, 1055
BciT130I CCWGG 1 cut(s) 596
BcnI CCSGG 2 cut(s) 806, 870
BcoDI GTCTC 2 cut(s) 49, 1120
BfaI CTAG 1 cut(s) 108
BfrI CTTAAG 1 cut(s) 299
BisI GCNGC 4 cut(s) 296, 758, 761, 810
BlsI GCNGC 4 cut(s) 297, 759, 762, 811
Bme1390I CCNGG 3 cut(s) 596, 806, 870
BmeT110I CYCGRG 1 cut(s) 722
BmgBI CACGTC 1 cut(s) 963
BmgT120I GGNCC 1 cut(s) 242
BmiI GGNNCC 1 cut(s) 412
BmrFI CCNGG 3 cut(s) 596, 806, 870
BmrI ACTGGG 1 cut(s) 484
BmsI GCATC 2 cut(s) 838, 1078
BmuI ACTGGG 1 cut(s) 484
BpiI GAAGAC 3 cut(s) 611, 619, 1045
Bpu10I CCTNAGC 1 cut(s) 415
BpuMI CCSGG 2 cut(s) 806, 870
Bsa29I ATCGAT 1 cut(s) 279
BsaBI GATNNNNATC 2 cut(s) 740, 1166
BsaJI CCNNGG 1 cut(s) 1104
BsaXI ACNNNNNCTCC 2 cut(s) 1011, 1041
Bsc4I CCNNNNNNNGG 2 cut(s) 495, 774
Bse1I ACTGG 6 cut(s) 201, 218, 320, 412, 490, 568
Bse8I GATNNNNATC 2 cut(s) 740, 1166
BseBI CCWGG 1 cut(s) 596
BseCI ATCGAT 1 cut(s) 279
BseDI CCNNGG 1 cut(s) 1104
BseGI GGATG 3 cut(s) 141, 510, 1069
BseJI GATNNNNATC 2 cut(s) 740, 1166
BseLI CCNNNNNNNGG 2 cut(s) 495, 774
BseMII CTCAG 2 cut(s) 14, 842
BseNI ACTGG 6 cut(s) 201, 218, 320, 412, 490, 568
BseRI GAGGAG 2 cut(s) 41, 476
BseXI GCAGC 4 cut(s) 307, 744, 747, 821
BseYI CCCAGC 1 cut(s) 122
BsgI GTGCAG 2 cut(s) 532, 748
BshFI GGCC 4 cut(s) 200, 217, 244, 599
BshVI ATCGAT 1 cut(s) 279
BsiHKAI GWGCWC 1 cut(s) 862
BsiHKCI CYCGRG 1 cut(s) 722
BsiSI CCGG 2 cut(s) 805, 870
BslFI GGGAC 3 cut(s) 435, 926, 973
BslI CCNNNNNNNGG 2 cut(s) 495, 774
BsmAI GTCTC 2 cut(s) 49, 1120
BsmBI CGTCTC 1 cut(s) 1120
BsmFI GGGAC 3 cut(s) 435, 926, 973
BsmI GAATGC 1 cut(s) 1109
BsnI GGCC 4 cut(s) 200, 217, 244, 599
BsoBI CYCGRG 1 cut(s) 722
Bsp1286I GDGCHC 1 cut(s) 862
Bsp143I GATC 5 cut(s) 126, 280, 331, 527, 1161
BspACI CCGC 2 cut(s) 419, 633
BspANI GGCC 4 cut(s) 200, 217, 244, 599
BspCNI CTCAG 2 cut(s) 15, 841
BspDI ATCGAT 1 cut(s) 279
BspLI GGNNCC 1 cut(s) 412
BspPI GGATC 4 cut(s) 134, 326, 535, 1156
BspTI CTTAAG 1 cut(s) 299
BsrI ACTGG 6 cut(s) 201, 218, 320, 412, 490, 568
BssECI CCNNGG 1 cut(s) 1104
BssMI GATC 5 cut(s) 126, 280, 331, 527, 1161
BssNAI GTATAC 1 cut(s) 395
BssT1I CCWWGG 1 cut(s) 1104
Bst1107I GTATAC 1 cut(s) 395
Bst2UI CCWGG 1 cut(s) 596
Bst4CI ACNGT 3 cut(s) 680, 769, 1158
BstAFI CTTAAG 1 cut(s) 299
BstC8I GCNNGC 1 cut(s) 1067
BstDEI CTNAG 4 cut(s) 23, 37, 415, 828
BstF5I GGATG 3 cut(s) 141, 510, 1069
BstKTI GATC 5 cut(s) 129, 283, 334, 530, 1164
BstMAI GTCTC 2 cut(s) 49, 1120
BstMBI GATC 5 cut(s) 126, 280, 331, 527, 1161
BstMWI GCNNNNNNNGC 4 cut(s) 383, 519, 857, 1106
BstNI CCWGG 1 cut(s) 596
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 3 cut(s) 594, 804, 868
BstV1I GCAGC 4 cut(s) 307, 744, 747, 821
BstV2I GAAGAC 3 cut(s) 611, 619, 1045
BstZ17I GTATAC 1 cut(s) 395
Bsu15I ATCGAT 1 cut(s) 279
BsuRI GGCC 4 cut(s) 200, 217, 244, 599
BsuTUI ATCGAT 1 cut(s) 279
BtrI CACGTC 1 cut(s) 963
BtsCI GGATG 3 cut(s) 141, 510, 1069
BtsI GCAGTG 1 cut(s) 1089
BtsIMutI CAGTG 2 cut(s) 194, 1089
Cac8I GCNNGC 1 cut(s) 1067
Cfr13I GGNCC 1 cut(s) 242
ClaI ATCGAT 1 cut(s) 279
Csp6I GTAC 1 cut(s) 791
CviAII CATG 4 cut(s) 18, 362, 425, 748
CviQI GTAC 1 cut(s) 791
DdeI CTNAG 4 cut(s) 23, 37, 415, 828
DpnI GATC 5 cut(s) 128, 282, 333, 529, 1163
DpnII GATC 5 cut(s) 126, 280, 331, 527, 1161
EaeI YGGCCR 3 cut(s) 198, 215, 597
Ecl136II GAGCTC 1 cut(s) 860
Eco130I CCWWGG 1 cut(s) 1104
Eco24I GRGCYC 1 cut(s) 862
Eco53kI GAGCTC 1 cut(s) 860
Eco57I CTGAAG 2 cut(s) 1098, 1152
Eco88I CYCGRG 1 cut(s) 722
EcoICRI GAGCTC 1 cut(s) 860
EcoRII CCWGG 1 cut(s) 594
EcoT14I CCWWGG 1 cut(s) 1104
EcoT38I GRGCYC 1 cut(s) 862
ErhI CCWWGG 1 cut(s) 1104
Esp3I CGTCTC 1 cut(s) 1120
FaeI CATG 4 cut(s) 21, 365, 428, 751
FaqI GGGAC 3 cut(s) 435, 926, 973
FatI CATG 4 cut(s) 17, 361, 424, 747
FauI CCCGC 1 cut(s) 412
FauNDI CATATG 1 cut(s) 220
FblI GTMKAC 1 cut(s) 394
Fnu4HI GCNGC 4 cut(s) 296, 758, 761, 810
FokI GGATG 3 cut(s) 148, 517, 1056
FriOI GRGCYC 1 cut(s) 862
Fsp4HI GCNGC 4 cut(s) 296, 758, 761, 810
FspBI CTAG 1 cut(s) 108
GluI GCNGC 4 cut(s) 296, 758, 761, 810
GsaI CCCAGC 1 cut(s) 126
HaeIII GGCC 4 cut(s) 200, 217, 244, 599
HapII CCGG 2 cut(s) 805, 870
Hin1II CATG 4 cut(s) 21, 365, 428, 751
HincII GTYRAC 3 cut(s) 163, 370, 892
HindII GTYRAC 3 cut(s) 163, 370, 892
HindIII AAGCTT 1 cut(s) 520
HinfI GANTC 6 cut(s) 255, 316, 433, 925, 942, 1002
HpaII CCGG 2 cut(s) 805, 870
HphI GGTGA 4 cut(s) 113, 622, 715, 806
Hpy166II GTNNAC 6 cut(s) 91, 163, 370, 395, 892, 1087
Hpy188I TCNGA 5 cut(s) 24, 149, 438, 735, 947
Hpy188III TCNNGA 1 cut(s) 922
Hpy8I GTNNAC 6 cut(s) 91, 163, 370, 395, 892, 1087
HpyAV CCTTC 2 cut(s) 255, 318
HpyCH4III ACNGT 3 cut(s) 680, 769, 1158
HpyCH4IV ACGT 2 cut(s) 962, 1089
HpyCH4V TGCA 5 cut(s) 513, 676, 729, 1011, 1069
HpyF10VI GCNNNNNNNGC 4 cut(s) 383, 519, 857, 1106
HpyF3I CTNAG 4 cut(s) 23, 37, 415, 828
HpySE526I ACGT 2 cut(s) 962, 1089
Hsp92II CATG 4 cut(s) 21, 365, 428, 751
Kzo9I GATC 5 cut(s) 126, 280, 331, 527, 1161
LmnI GCTCC 2 cut(s) 416, 686
Lsp1109I GCAGC 4 cut(s) 307, 744, 747, 821
LweI GCATC 2 cut(s) 838, 1078
MaeI CTAG 1 cut(s) 108
MaeII ACGT 2 cut(s) 962, 1089
MaeIII GTNAC 4 cut(s) 466, 539, 569, 986
MalI GATC 5 cut(s) 128, 282, 333, 529, 1163
MboI GATC 5 cut(s) 126, 280, 331, 527, 1161
MboII GAAGA 5 cut(s) 275, 611, 619, 763, 1045
MhlI GDGCHC 1 cut(s) 862
MlsI TGGCCA 3 cut(s) 200, 217, 599
MluCI AATT 4 cut(s) 354, 454, 695, 823
MluNI TGGCCA 3 cut(s) 200, 217, 599
MlyI GAGTC 1 cut(s) 442
MmeI TCCRAC 3 cut(s) 1097, 1166, 1177
MnlI CCTC 9 cut(s) 18, 59, 62, 246, 454, 457, 950, 986, 1013
Mox20I TGGCCA 3 cut(s) 200, 217, 599
MscI TGGCCA 3 cut(s) 200, 217, 599
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 1 cut(s) 724
Msp20I TGGCCA 3 cut(s) 200, 217, 599
MspCI CTTAAG 1 cut(s) 299
MspI CCGG 2 cut(s) 805, 870
MspR9I CCNGG 3 cut(s) 596, 806, 870
Mva1269I GAATGC 1 cut(s) 1109
MvaI CCWGG 1 cut(s) 596
MwoI GCNNNNNNNGC 4 cut(s) 383, 519, 857, 1106
NciI CCSGG 2 cut(s) 806, 870
NdeI CATATG 1 cut(s) 220
NdeII GATC 5 cut(s) 126, 280, 331, 527, 1161
NlaIII CATG 4 cut(s) 21, 365, 428, 751
NlaIV GGNNCC 1 cut(s) 412
NspI RCATGY 1 cut(s) 428
OliI CACNNNNGTG 1 cut(s) 724
PciI ACATGT 1 cut(s) 424
PctI GAATGC 1 cut(s) 1109
PfeI GAWTC 5 cut(s) 255, 316, 925, 942, 1002
PkrI GCNGC 4 cut(s) 297, 759, 762, 811
PleI GAGTC 1 cut(s) 441
PpsI GAGTC 1 cut(s) 441
PscI ACATGT 1 cut(s) 424
Psp124BI GAGCTC 1 cut(s) 862
Psp1406I AACGTT 1 cut(s) 1089
Psp6I CCWGG 1 cut(s) 594
PspFI CCCAGC 1 cut(s) 122
PspGI CCWGG 1 cut(s) 594
PspN4I GGNNCC 1 cut(s) 412
PspPI GGNCC 1 cut(s) 242
RsaI GTAC 1 cut(s) 792
RsaNI GTAC 1 cut(s) 791
RseI CAYNNNNRTG 1 cut(s) 724
SacI GAGCTC 1 cut(s) 862
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 4 cut(s) 296, 758, 761, 810
Sau3AI GATC 5 cut(s) 126, 280, 331, 527, 1161
Sau96I GGNCC 1 cut(s) 242
SchI GAGTC 1 cut(s) 442
ScrFI CCNGG 3 cut(s) 596, 806, 870
SduI GDGCHC 1 cut(s) 862
SfaNI GCATC 2 cut(s) 838, 1078
SmiMI CAYNNNNRTG 1 cut(s) 724
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
Sse9I AATT 4 cut(s) 354, 454, 695, 823
SsiI CCGC 2 cut(s) 419, 633
SspMI CTAG 1 cut(s) 108
SstI GAGCTC 1 cut(s) 862
StyD4I CCNGG 3 cut(s) 594, 804, 868
StyI CCWWGG 1 cut(s) 1104
TaaI ACNGT 3 cut(s) 680, 769, 1158
TaiI ACGT 2 cut(s) 965, 1092
TaqI TCGA 3 cut(s) 279, 399, 459
TasI AATT 4 cut(s) 354, 454, 695, 823
TfiI GAWTC 5 cut(s) 255, 316, 925, 942, 1002
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 2 cut(s) 201, 1089
TseI GCWGC 4 cut(s) 295, 757, 760, 809
TspDTI ATGAA 5 cut(s) 22, 350, 636, 764, 815
TspGWI ACGGA 1 cut(s) 1005
TspRI CASTG 2 cut(s) 201, 1089
Vha464I CTTAAG 1 cut(s) 299
XapI RAATTY 2 cut(s) 454, 695
XceI RCATGY 1 cut(s) 428
XcmI CCANNNNNNNNNTGG 1 cut(s) 497
XmiI GTMKAC 1 cut(s) 394
XspI CTAG 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.